scholarly journals Morphological changes in the rat’s liver of different age after administration of magnesium chloride

2019 ◽  
pp. 40-48
Author(s):  
Roman V. Yanko ◽  
Elena G. Chaka ◽  
Irina G. Litovka ◽  
Mikhail I. Levashov
Keyword(s):  
Magnesium Chloride ◽  
Morphological Changes ◽  
Serum Magnesium ◽  
Liver Parenchyma ◽  
Magnesium Content ◽  
Male Rats ◽  
Standard Diet ◽  
Erythrocyte Suspension ◽  
Physiological Regeneration ◽  
Different Ages

The aim of the work was to study the morphological changes in the rat’s liver of different ages after prolonged administration of magnesium chloride. The experiments were performed on 48 Wistar male rats at 3 and 15 months of age. Experi mental animals, in addition to the standard diet, received magnesium chloride daily for 21 days at a dose of 50 mg / kg of body weight. Histological preparations were made from the liver tissue according to the standard method. The liver morphometry was performed on digital images using the computer program Image J. The content of magnesium cations was determined in the serum and suspension of red blood cells. Based on the results of our studies, it can be assumed that the administration of magnesium chloride activates the processes of physiological regeneration and functional activity of the liver parenchyma in rats of different ages. This is evidenced by an increase in the number of binuclear cells and nucleolus in the nucleus of hepatocytes, an increase in the nuclear-cytoplasmic and nucleolar-nuclear ratio. The relative area of the sinusoid network, the number and density of connective tissue cells were increased in the liver of 3-month-old experimental rats. This may indicate an increase of trophic and protective activity of the stroma in this organ. Experimental rats (15-month-old animals) revealed a moderate increasing in the erythrocyte suspension and blood serum magnesium content. The administration of magnesium chloride has the morphological features indicating an increasing of the physiological regeneration and activity of the liver parenchyma in young (to a greater extent) and adult animals.

scholarly journals Morphofunctional changes in the rat's liver of different ages after L-methionine administration

2022 ◽  
Vol 12 (1) ◽  
pp. 125-137
Author(s):  
Roman Yanko ◽  
Elena Chaka ◽  
Mikhail Levashov
Keyword(s):  
Succinate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Protein Concentration ◽  
Morphological Changes ◽  
Liver Parenchyma ◽  
Synthetic Activity ◽  
Standard Diet ◽  
Energy Potential ◽  
Succinate Dehydrogenase Activity ◽  
Different Ages

Background: Literature data on the effect of methionine on functional activity and, especially, on morphological changes in the liver parenchyma in animals of different ages are sporadic, and research results are often ambiguous. Aim: The purpose of this work was to study and compare the morphofunctional changes in the liver of rats of different ages on prolonged administration of L-methionine. Material and Methods: The experiment was performed on 48 male Wistar rats of 3 and 15 months of age. Animals of the experimental group received L-methionine at a dose of 250 mg/kg body weight in addition to the standard diet, daily for 21 days. Histological preparations were prepared from liver tissue by a standard technique. Morphometry was performed on digital images using the computer program «Image J». Succinate dehydrogenase activity and protein concentration were determined in the suspension of hepatocyte mitochondria. Results: It was revealed that 21-day administration of L-methionine to rats led to hypertrophy of the hepatocyte nucleus, an increase in the nuclear-cytoplasmic ratio, the number of binuclear hepatocytes, and the nucleolus in the cell nucleus. The relative area of ​​the sinusoids network increased by 50% in 3-month-old animals. This indicated a better blood filling of the liver parenchyma. The increase in succinate dehydrogenase activity and protein concentration was revealed in the suspension of hepatocyte mitochondria of the experimental rats. This indicated an increase in the mitochondria energy potential and protein-synthetic activity. Conclusions: The administration of prophylactic doses of methionine to healthy rats leads to the appearance of pronounced morphological and functional signs of increased activity of hepatocytes. The severity of this effect has a distinct age-dependent character. In young rats, it is more pronounced than in mature rats. The results of the study are important for practical medicine when using methionine for therapeutic and prophylactic purposes.

MECHANISM OF THE HYPOCALCAEMIC ACTION OF PARENTERALLY ADMINISTERED MAGNESIUM

1972 ◽  
Vol 70 (3) ◽  
pp. 476-486 ◽  
Author(s):  
S. Pors Nielsen ◽  
F. Schønau Jørgensen
Keyword(s):  
Serum Concentration ◽  
Magnesium Chloride ◽  
Subcutaneous Injection ◽  
Calcium Concentration ◽  
Renal Excretion ◽  
Serum Magnesium ◽  
Bone Matrix ◽  
Male Rats ◽  
Serum Calcium Concentration

ABSTRACT Subcutaneous injection of 300 μmol magnesium raised the serum magnesium to 4.00 mmol/l after 30 min in 150 g male rats and reduced the serum calcium concentration by 0.17 mmol/l after 60 min. Magnesium also acutely diminished the serum concentration of 45Ca given three weeks previously. The administration of magnesium did not reduce the serum concentration of 45Ca and did not increase the rate of disappearance of 45Ca from the serum, when subcutaneous or intravenous labelling took place 180–210 min before the magnesium injection. The magnesium-induced hypocalcaemia therefore cannot be explained by an increased removal of calcium from the circulation. The results are compatible with the hypothesis that the magnesium-induced hypocalcaemia is caused by the release of calcitonin. Injections of 300 μmol magnesium chloride at 12 h intervals for 72 h trebled the 24 h renal excretion of both 40Ca and 45Ca given three weeks previously. The 24 h renal excretion of hydroxyproline was unaltered. Thus there was no indication that the intermittent magnesium injections produced any long-term inhibition of the turnover of bone matrix and bone mineral.

EFFECT OF INTERVAL FASTING ON MORPHOLOGICAL CHANGES IN THE RAT THYROID GLAND OF DIFFERENT AGE

2021 ◽  
pp. 8-18
Author(s):  
R.V. Yanko ◽  
◽  
M.I. Levashov ◽  
Keyword(s):  
Thyroid Gland ◽  
Morphological Changes ◽  
Thyroid Tissue ◽  
The Body ◽  
Follicular Epithelium ◽  
Synthetic Activity ◽  
Standard Diet ◽  
Different Ages ◽  
Old Rats ◽  
Rat Thyroid

Despite of the well-studied effect of interval fasting on the body, literature data on its infl uence on functional activity and, especially, on morphological changes in the thyroid gland are sporadic. The research results are often contradictory, which may be due to differences in experimental conditions. The aim of this study was to investigate the morphological changes in the rat’s thyroid gland of different ages, which were on interval fasting. The experiments were performed on 48 male Wistar rats aged 4 and 16 months. The experimental rats underwent interval fasting (1 day complete fasting / 2 days standard diet). Duration of experiment was 28 days. Thyroid tissue preparations were made according to standard histological methods. The morphometry was performed on digital images using a computer program Image J. It was found that the colloid area, the interior diameter of the follicles and colloid accumulation index were decreased in 4 and 16 month-old rats after interval fasting. The relative connective tissue area was also decreased in 16-month- old rats. But the follicular epithelium height, the follicular-colloidal index and the number of interfollicular islets were increased. The morphological changes of the thyroid gland tissue in 16-month-old experimental rats were manifested to a greater extent than in young animals. Thus, after exposure to interval fasting, morphological signs of activation of the thyroid gland synthetic activity in rats of different ages were observed. Key words: interval fasting, thyroid gland, morphometric indicators.

PROTECTIVE EFFECT OF SOME SALTS 2-AMINOETHANESULFONIC ACID IN AN EXPERIMENTAL MODEL OF DEGENERATIVE SPINAL CORD INJURY

2020 ◽  
pp. 41-47
Author(s):  
Semeleva E.V. ◽  
Blinova E.V. ◽  
Zaborovsky A.V. ◽  
Vasilkina O.V. ◽  
Shukurov A.S.
Keyword(s):  
Spinal Cord ◽  
Morphological Changes ◽  
Male Rats ◽  
Zinc Salt ◽  
Control Group ◽  
Morphological Studies ◽  
Cord Injury ◽  
The Central Nervous System ◽  
Acid Compound ◽  
Lateral Sclerosis

In this work, we studied the pharmacological activity of zinc and magnesium salts of 2-aminoethanesulfonic acid in white non-linear male rats with amyotrophic lateral sclerosis, which was modeled by neurotoxicantsimplication into the pelvic part of spinal cord. After the reproduction of the pathology in animals, the indices of motor activity were recorded in the Rotarod test, and morphological studies of spinal cord sections stained according to Nisl in the Belshovsky modification were carried out. It was shown that the magnesium salt of 2-aminoethanesulfonic acid (compound LHT-317) to a greater extent reduces the development of motor disorders in experimental animals compared with the control group on the 4th day of observation. The course of intravenous administration of the studied compounds of 2-aminoethanesulfonic acid did not inhibit morphological changes in the spinal cord that develop in degenerative-dystrophic pathology of the central nervous system: connections. Moreover, if, against the background of treatment with zinc salt, the total area of motor zones in animals of the experimental group exceeded that of control rats, then the number of motoneurons did not differ from the control.

scholarly journals MAGNESIUM CONTENT OF NORMAL RATS AT DIFFERENT AGES

1927 ◽  
Vol 74 (1) ◽  
pp. 149-151 ◽  
Author(s):  
Grace Medes ◽  
Gertrude J. Humphrey
Keyword(s):  
Magnesium Content ◽  
Different Ages

REACTIVE CHANGES OF LIVER AND KIDNEY HISTOLOGICAL ELEMENTS IN MURINE MODELS OF SEPSIS CAUSED BY ADMINISTRATION OF DIFFERENT STRAINS TO MICE

2020 ◽  
pp. 66-71
Author(s):  
Татьяна Геннадьевна Боровая ◽  
Владимир Григорьевич Жуховицкий ◽  
Мария Николаевна Черкасова
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Morphological Changes ◽  
Blood Stasis ◽  
Intraperitoneal Administration ◽  
Liver Parenchyma ◽  
Experimental Models ◽  
Control Group ◽  
Reactive Changes ◽  
Liver And Kidney ◽  
Different Strains

Цель - выявление реактивных изменений гистологических элементов печени и почек у мышей в экспериментальных моделях сепсиса, вызванного штаммами 1840 и 1623 Pseudomonas aeruginosa (PsA1840, 1623). Материал и методы. Сепсис моделировали на двух группах половозрелых самцов мышей линии C57Bl/6 интраперитонеальным введением Pseudomonas aeruginosa. Животным 1-й группы (8 особей) вводили штамм 1840, животным 2-й группы (12 особей) - штамм 1623. Контрольная группа состояла из 3 животных. Перед началом опыта штаммы тестировали на присутствие генов экзотоксинов U, S, T, Y (ExoU, ExoS, ExoT, ExoY) с помощью полимеразной цепной реакции (ПЦР). Для визуализации продуктов ПЦР применяли электрофорез в горизонтальном 1,5 % агарозном геле. Животных вскрывали на терминальной стадии сепсиса. Серийные парафиновые срезы печени и почек толщиной 4 мкм окрашивали гематоксилином - эозином, анализировали особенности гистоструктуры органов и фотографировали в световом микроскопе «AxioPlus» (фирма Zeiss, Германия). Результаты. Штамм PsA 1840, имеющий ген exoU, вызывал выраженные деструктивные изменения пластинок гепатоцитов и замещение участков паренхимы печени гомогенным эозинофильным веществом. Присутствовали признаки стаза крови в синусоидных капиллярах, расширение и тромбоз центральных вен, немногочисленные скопления лейкоцитов. Морфологические изменения нефронов состояли в локальных деструктивных изменениях проксимальных канальцев на периферии коркового вещества почек. При введении PsA1623, имеющего ген exoS, возникали массовая гибель почечных телец и дегенерация канальцев нефронов. В печени дольковая гистоархитектура в основном сохранялась. Выводы. Предполагается связь выявленных различий в реактивных изменениях гистологических элементов печени и почек в подопытных группах с особенностями геномов штаммов PsA, использованных для моделирования сепсиса. Objective - to identify reactive changes of liver and kidney histological elements in experimental models of sepsis in mice caused by 1840 and 1623 Pseudomonas aeruginosa strains (PsA1840, 1623). Material and methods. Sepsis was modeled in two groups of mature male C57Bl/6 mice by intraperitoneal administration of Pseudomonas aeruginosa. Strain 1840 was administered to animals of the first group (n=8), animals of the second group (n=12) were administered strain 1623; the control group consisted of 3 animals. Before the experiment, the strains were tested for the presence of genes of exotoxins U, S, T, Y (ExoU, ExoS, ExoT, ExoY) using polymerase chain reaction (PCR). Electrophoresis in horizontal 1,5 % agarose gel was used to visualize PCR products. The animals were euthanized at the terminal stage of sepsis. The extracted liver and kidneys were fixed according to the generally accepted histological method, and embedded into paraffin blocks. Serial 4 μm thick sections of organs were stained with hematoxylin and eosin, analyzed and photographed using «AxioPlus» light microscope (Zeiss, Germany). Results. Strain PsA 1840, carrying the gene of exotoxin U (ExoU), caused severe destructive changes of hepatocytes plates and the replacement of the liver parenchyma with homogeneous eosinophilic substance. There were signs of blood stasis in sinusoidal capillaries, expansion and thrombosis of central veins, a few accumulations of leukocytes. Morphological changes of nephrons consisted of local destructive changes in the proximal tubules at the periphery of kidney cortical substance. After the introduction of PsA1623, carrying the gene of exotoxin S (ExoS), the massive death of renal corpuscles and degeneration of nephron tubules were registered. However, the lobular histoarchitecture in the liver remained mostly unaltered. Conclusions. It is supposed that there is a possible connection of the observed differences in reactive changes of liver and kidney histological elements in two experimental groups with genome features of PsA strains used for the sepsis modeling.

Hair Magnesium content and its relation to Serum Magnesium level in children with Idiopathic Epilepsy

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Hamed Mahmoud Shatla ◽  
Mariam Fathy Abdel Maksoud ◽  
Raghda Mohamed Hesham Zaitoun ◽  
Alaa Rabie Abdel Baset Mahmoud
Keyword(s):  
Serum Magnesium ◽  
Serum Levels ◽  
Magnesium Content ◽  
Magnesium Level ◽  
Idiopathic Epilepsy ◽  
Age And Gender ◽  
Control Subjects ◽  
Significant Difference ◽  
And Gender ◽  
Patients With Epilepsy

Abstract Objective To measure the level of hair Mg, as well as its level in serum, in patients with epilepsy and compare them to the levels found in non-epileptic age and gender matched children, and to explore any potential correlation between either serum or hair level of magnesium and seizure characteristics in children with idiopathic epilepsy. Methods An observational cross-sectional study including 50 children with idiopathic epilepsy and 100 non-epileptic age and gender matched control subjects. Cases were subjected to full history taking, examination and measurements of serum and hair levels of magnesium, control subjects only had their serum and hair level of magnesium measured as for the cases. Results The mean serum magnesium was 29.11 ± 13.42 ug/ml for cases and 27.67 ± 7.24 ug/ml for controls and the median hair level of magnesium was 42.22 ug/g with IQR of 25.9 - 56.82 for cases and 38.6 ug/g with IQR of 25.21 - 61.25 for controls. No statistically significant difference was observed between both groups as regards either serum or hair magnesium levels. No statistically significant correlation was observed between either hair or serum levels of magnesium and seizure characteristics though the correlations were nearing statistical significance for the hair magnesium content. Conclusion Hair magnesium level may be better correlated to seizure characteristics and control than serum levels in patients with epilepsy.

Mechanism of increased erythrocyte membrane fluidity during magnesium deficiency in weanling rats

1989 ◽  
Vol 257 (2) ◽  
pp. C270-C276 ◽  
Author(s):  
S. Tongyai ◽  
Y. Rayssiguier ◽  
C. Motta ◽  
E. Gueux ◽  
P. Maurois ◽  
...  
Keyword(s):  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Magnesium Deficiency ◽  
Osmotic Fragility ◽  
Magnesium Content ◽  
Male Rats ◽  
Surface Irregularities ◽  
Erythrocyte Membrane Fluidity ◽  
And Control

The erythrocyte membrane was investigated in weanling male rats pair fed with magnesium-deficient and control diets for 8 days. Fluorescence polarization studies revealed a 15% increase in the fluidity of membranes from deficient rats. A similar increase in the fluidity of liposomes indicated that protein was not involved. The change was associated with decreased osmotic fragility of intact erythrocytes; the cells lost their biconcavity and had a flattened appearance with surface irregularities. Analysis of the membranes showed decreased amounts of magnesium, cholesterol, and sphingomyelin in the deficient group. The reduced ratios of cholesterol to phospholipid and sphingomyelin to phosphatidylcholine were consistent with the increased fluidity. Addition of physiological amounts of magnesium to the medium rigidified membranes incubated in tris(hydroxymethyl)-aminomethane buffer, and this was prevented by the presence of EDTA. Cross-incubation experiments with erythrocyte ghosts and plasma from the two groups of rats showed that magnesium-deficient plasma increased the fluidity of control ghosts and control plasma rigidified ghosts from magnesium-deficient rats. Addition of sufficient magnesium chloride to raise the magnesium content of deficient plasma to normal had no significant effect. These results show that the increased fluidity of the erythrocyte membrane in magnesium deficiency is due to physicochemical exchange with the plasma. Although magnesium can directly influence membrane fluidity, the change during its deficiency in vivo is mainly mediated indirectly via disturbances in lipid metabolism.

scholarly journals The effects of supraphysiological supplementation of b-carotene in spontaneously hypertensive rats (SHR and SHR-sp)

2014 ◽  
Vol 41 (5) ◽  
pp. 351-355 ◽  
Author(s):  
Stênio Karlos Alvim Fiorelli ◽  
Lúcia Marques Vianna ◽  
Carlos Alberto Basílio de Oliveira ◽  
Rossano Kepler Alvim Fiorelli ◽  
Bernardo Cunha Senra Barros ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Spontaneously Hypertensive Rats ◽  
Carotid Arteries ◽  
Morphological Changes ◽  
Serum Levels ◽  
Muscle Layer ◽  
Male Rats ◽  
Elastic Fibers ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive

Objective: to investigate the effect of administration of supraphysiological âcaroteno on biological parameters (ectoscopy and blood pressure), laboratory (malondialdehyde) and histological (liver and carotid arteries) of spontaneously hypertensive rats prone to stroke (SHR-sp).Methods: we used 36 male rats were divided into three groups, each containing 12 rats Wistar, SHR and SHR-sp, subdivided into six control animals and six animals treated with supraphysiological doses of âcaroteno for two periods of ten weeks interspersed with one week interruption. In the experiment were assessed daily physical examination and blood pressure (plethysmography). At sacrifice, blood was collected for measurement of serum malondialdehyde, liver and carotid arteries for histological examination.Results: temporary change in color of the fur, decreased significantly (p<0.0001) blood pressure (20mg supplementation âcaroteno) and serum levels of malondialdehyde (p<0.05) and increased amount of elastic fibers in the carotid wall of SHR and SHR-sp.Conclusion: supplementation of supraphysiological âcaroteno caused no toxic effects, showed positive response in the modulation of blood pressure and lower serum malondialdehyde. No significant morphological changes were found in both groups, except an increase in the number of elastic fibers in the muscle layer carotid suggesting elastosis in SHR and SHR-sp.

Time window-dependent effect of perinatal maternal protein restriction on insulin sensitivity and energy substrate oxidation in adult male offspring

2014 ◽  
Vol 307 (2) ◽  
pp. R184-R197 ◽  
Author(s):  
Aurore Martin Agnoux ◽  
Jean-Philippe Antignac ◽  
Gilles Simard ◽  
Guillaume Poupeau ◽  
Dominique Darmaun ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Insulin Sensitivity ◽  
Adult Male ◽  
Time Window ◽  
Substrate Oxidation ◽  
Male Rats ◽  
Testable Hypothesis ◽  
Integrative Approach ◽  
Composition Analysis ◽  
Standard Diet

Epidemiological and experimental evidence suggests that a suboptimal environment during perinatal life programs offspring susceptibility to the development of metabolic syndrome and Type 2 diabetes. We hypothesized that the lasting impact of perinatal protein deprivation on mitochondrial fuel oxidation and insulin sensitivity would depend on the time window of exposure. To improve our understanding of underlying mechanisms, an integrative approach was used, combining the assessment of insulin sensitivity and untargeted mass spectrometry-based metabolomics in the offspring. A hyperinsulinemic-euglycemic clamp was performed in adult male rats born from dams fed a low-protein diet during gestation and/or lactation, and subsequently exposed to a Western diet (WD) for 10 wk. Metabolomics was combined with targeted acylcarnitine profiling and analysis of liver gene expression to identify markers of adaptation to WD that influence the phenotype outcome evaluated by body composition analysis. At adulthood, offspring of protein-restricted dams had impaired insulin secretion when fed a standard diet. Moreover, rats who demonstrated catch-up growth at weaning displayed higher gluconeogenesis and branched-chain amino acid catabolism, and lower fatty acid β-oxidation compared with control rats. Postweaning exposure of intrauterine growth restriction-born rats to a WD exacerbated incomplete fatty acid β-oxidation and excess fat deposition. Control offspring nursed by protein-restricted mothers showed peculiar low-fat accretion through adulthood and preserved insulin sensitivity even after WD-exposure. Altogether, our findings suggest a testable hypothesis about how maternal diet might influence metabolic outcomes (insulin sensitivity) in the next generation such as mitochondrial overload and/or substrate oxidation inflexibility dependent on the time window of perinatal dietary manipulation.

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