scholarly journals Gene Editing in Rabbits: Unique Opportunities for Translational Biomedical Research

2021 ◽  
Vol 12 ◽  
Author(s):  
Jie Xu ◽  
Jifeng Zhang ◽  
Dongshan Yang ◽  
Jun Song ◽  
Brooke Pallas ◽  
...  
Keyword(s):  
Animal Model ◽  
Biomedical Research ◽  
Gene Editing ◽  
Gene Knockout ◽  
Ocular Diseases ◽  
The Past ◽  
Research Areas ◽  
Large Size ◽  
Transgenic Rabbits ◽  
Better Than

The rabbit is a classic animal model for biomedical research, but the production of gene targeted transgenic rabbits had been extremely challenging until the recent advent of gene editing tools. More than fifty gene knockout or knock-in rabbit models have been reported in the past decade. Gene edited (GE) rabbit models, compared to their counterpart mouse models, may offer unique opportunities in translational biomedical research attributed primarily to their relatively large size and long lifespan. More importantly, GE rabbit models have been found to mimic several disease pathologies better than their mouse counterparts particularly in fields focused on genetically inherited diseases, cardiovascular diseases, ocular diseases, and others. In this review we present selected examples of research areas where GE rabbit models are expected to make immediate contributions to the understanding of the pathophysiology of human disease, and support the development of novel therapeutics.

scholarly journals Use of Customizable Nucleases for Gene Editing and Other Novel Applications

Genes ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 976
Author(s):  
Pradeep Reddy ◽  
Felipe Vilella ◽  
Juan Carlos Izpisua Belmonte ◽  
Carlos Simón
Keyword(s):  
Animal Models ◽  
Genome Editing ◽  
Biomedical Research ◽  
Laboratory Animal ◽  
Gene Editing ◽  
Genetic Disorders ◽  
Basic Research ◽  
The Past ◽  
Novel Applications ◽  
In Cells

The development of novel genome editing tools has unlocked new opportunities that were not previously possible in basic and biomedical research. During the last two decades, several new genome editing methods have been developed that can be customized to modify specific regions of the genome. However, in the past couple of years, many newer and more exciting genome editing techniques have been developed that are more efficient, precise, and easier to use. These genome editing tools have helped to improve our understanding of genetic disorders by modeling them in cells and animal models, in addition to correcting the disease-causing mutations. Among the genome editing tools, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system has proven to be the most popular one due to its versatility and has been successfully used in a wide variety of laboratory animal models and plants. In this review, we summarize the customizable nucleases currently used for genome editing and their uses beyond the modification of genome. We also discuss the potential future applications of gene editing tools for both basic research and clinical purposes.

Ultrastructure and analytical microprobe analysis of simian lung mite pigments

1986 ◽  
Vol 44 ◽  
pp. 324-325
Author(s):  
R. W. Cole ◽  
J. C. Kim
Keyword(s):  
Biomedical Research ◽  
Tissue Damage ◽  
Microprobe Analysis ◽  
Old World Monkeys ◽  
Old World ◽  
Experimental Animals ◽  
The Past ◽  
Lung Mite ◽  
Mite Infestations ◽  
Cardiopulmonary System

In recent years, non-human primates have become indispensable as experimental animals in many fields of biomedical research. Pharmaceutical and related industries alone use about 2000,000 primates a year. Respiratory mite infestations in lungs of old world monkeys are of particular concern because the resulting tissue damage can directly effect experimental results, especially in those studies involving the cardiopulmonary system. There has been increasing documentation of primate parasitology in the past twenty years.

Implementasi Algoritma Hamming Distance dan Brute Force dalam Mendeteksi Kemiripan Source Code Bahasa Pemrograman C

2017 ◽  
Vol 8 (2) ◽  
Author(s):  
Andreas Budiman ◽  
Dennis Gunawan ◽  
Seng Hansun
Keyword(s):  
College Presidents ◽  
Hamming Distance ◽  
Source Code ◽  
Structural Type ◽  
Brute Force ◽  
Important Thing ◽  
The Past ◽  
Index Terms ◽  
Better Than

Plagiarism is a behavior that causes violence of copyrights. Survey shows 55% of college presidents say that plagiarism in students’ papers has increased over the past 10 years. Therefore, an application for detecting plagiarism is needed, especially for teachers. This plagiarism checker application is made by using Visual C# 2010. The plagiarism checker uses hamming distance algorithm for matching line code of the source code. This algorithm works by matching the same length string of the code programs. Thus, it needs brute will be matched with hamming distance. Another important thing for detecting plagiarism is the preprocessing, which is used to help the algorithm for detecting plagiarized source code. This paper shows that the application works good in detecting plagiarism, the hamming distance algorithm and brute force algorithm works better than levenstein distance algorithm for detecting structural type of plagiarism and this thesis also shows that the preprocessing could help the application to increase its percentage and its accuracy. Index Terms—Brute Force, Hamming Distance, Plagiarisme, Preprocessing.

Ways of Being and Time

2017 ◽  
Author(s):  
Kris McDaniel
Keyword(s):  
Being And Time ◽  
Present Moment ◽  
Ontological Pluralism ◽  
The Past ◽  
Mode Of Being ◽  
Ways Of Being ◽  
Better Than

This chapter develops a version of ontological pluralism that respects two common intuitions about time: that the present moment is metaphysically distinguished but not in such a way that the past is unreal. The version of ontological pluralism developed—presentist existential pluralism (PEP)—embraces two modes of being, the mode of being that present objects enjoy and the mode of being that past objects enjoy. The author argues that this view fares at least as well, and probably better, than other views in which the present is metaphysically distinguished. The chapter also introduces another form of ontological superiority called “levels of being.”

Temporal Focus

2020 ◽  
Author(s):  
Abbie J. Shipp
Keyword(s):  
Well Being ◽  
Psychology Research ◽  
The Past ◽  
Research Areas ◽  
The Future ◽  
Behavior Based ◽  
The Individual ◽  
And Behavior ◽  
Temporal Focus

Temporal focus is the individual tendency to characteristically think more or less about the past, present, and future. Although originally rooted in early work from psychology, research on temporal focus has been steadily growing in a number of research areas, particularly since Zimbardo and Boyd’s (1999) influential article on the topic. This chapter will review temporal focus research from the past to the present, including how temporal focus has been conceptualized and measured, and which correlates and outcomes have been tested in terms of well-being and behavior. Based on this review, an agenda for research is created to direct temporal focus research in the future.

Taking Peritoneal Dialysis beyond the Year 2000

1999 ◽  
Vol 19 (3_suppl) ◽  
pp. 35-42 ◽  
Author(s):  
Ram Gokal
Keyword(s):  
Peritoneal Dialysis ◽  
Peritoneal Membrane ◽  
Membrane Function ◽  
Fluid Removal ◽  
The Past ◽  
First Choice ◽  
Year 2000 ◽  
Economic Considerations ◽  
Appropriate Prescription ◽  
Better Than

Over the past 25 years, peritoneal dialysis (PD) has steadily improved so that now its outcomes, in the form of patient survival, are equivalent to, and at times better than, those for hemodialysis. We now have a better understanding of the pathophysiology of peritoneal membrane function and damage and the importance of appropriate prescription to meet agreed-upon targets of solute and fluid removal. In the next millennium, greater emphasis will be put on prescription setting and subsequent monitoring. This will entail an increase in automated PD, especially for lifestyle reasons as well as for patients with a hyperpermeable peritoneal membrane. To improve outcomes, dialysis should be started earlier than is currently the case. It is easy to do this with PD, where an incremental approach is made easier by the introduction of icodextrin for long-dwell PD. In the future, solutions will be tailored to be more biocompatible and to provide improved nutrition and better cardiovascular outcomes. Finally, economic considerations favor PD, which is cheaper than in-centre hemodialysis. Thus, for many, PD has become a first-choice therapy, and with further improvements this trend will continue.

Frozen Negotiations: The Peace Process in Chiapas

2006 ◽  
Vol 22 (1) ◽  
pp. 131-152 ◽  
Author(s):  
Raúúl Beníítez Manaut ◽  
Andrew Selee ◽  
Cynthia J. Arnson
Keyword(s):  
Armed Conflict ◽  
Democratic Transition ◽  
Political Process ◽  
Peace Process ◽  
Democratic Society ◽  
The Political ◽  
Peace Agreement ◽  
The Past ◽  
The Government ◽  
Better Than

Mexico's democratic transition has helped reduce, if not eliminate, the threat of renewed armed conflict in Chiapas. However, absent more active measures from the government and the Ejéército Zapatista de Liberacióón Nacional (EZLN) to seek a permanent peace agreement and come to terms with the legacies of the past, the conflict will linger on in an unstable déétente, which we term ““armed peace.”” While this situation is far better than the open hostilities of the past, it also belies the promise of a fully democratic society in which all citizens are equally included in the political process. La transicióón democráática en Mééxico ha contribuido a reducir, si no eliminar, la posibilidad de que el conflicto armado en Chiapas se reanude. Sin embargo, sin esfuerzos mas activos por parte del gobierno y del Ejéército Zapatista de Liberacióón Nacional (EZLN) para buscar un acuerdo de paz permanente y saldar cuentas con el pasado, el conflicto permaneceráá en un estado inestable que llamamos ““paz armada””. Aunque esta situacióón es mucho mejor que las tensiones y agresiones del pasado, no cumple los requisitos de una sociedad plenamente democráática en que todos los ciudadanos participan en condiciones de igualdad en el proceso políítico.

scholarly journals Validation of SCIAMACHY limb NO<sub>2</sub> profiles using solar occultation measurements

2011 ◽  
Vol 4 (4) ◽  
pp. 4753-4800
Author(s):  
R. Bauer ◽  
A. Rozanov ◽  
C. A. McLinden ◽  
L. L. Gordley ◽  
W. Lotz ◽  
...  
Keyword(s):  
The Past ◽  
Middle Latitudes ◽  
Solar Occultation ◽  
Time Period ◽  
Vertical Distributions ◽  
Global Coverage ◽  
Scanning Imaging ◽  
Diurnal Effect ◽  
Better Than ◽  
Balloon Measurements

Abstract. The increasing amounts of reactive nitrogen in the stratosphere necessitates accurate global measurements of stratospheric nitrogen dioxide (NO2). Over the past decade, the SCIAMACHY (SCanning Imaging Absorption spectroMeter for Atmospheric CHartographY) instrument on ENVISAT (European Environmental Satellite) has been providing global coverage of stratospheric NO2 every 6 days, which is otherwise difficult to achieve with other systems (e.g. balloon measurements, solar occultation). In this study, the vertical distributions of NO2 retrieved from limb measurements of the scattered solar light from the SCIAMACHY instrument are validated using NO2 products from three different satellite instruments (SAGE II, HALOE and ACE-FTS). The retrieval approach, as well as the sensitivity of the SCIAMACHY NO2 limb data product are discussed, and the photochemical corrections needed to make this validation feasible, as well as the chosen collocation criteria are described. For each instrument, a time period of two years is analyzed with several hundreds of collocation pairs for each year and instrument. The agreement between SCIAMACHY and each instrument is found to be better than 10 % between 22–24 km and 40 km. Additionally, NO2 amounts in three different latitude regions are validated individually, with considerably better agreements in high and middle latitudes compared to tropics. Differences with SAGE II and ACE-FTS below 20 km are consistent with those expected from the diurnal effect.

An Improved Method for CT Image Coding Using PSNR Prediction Model Based on ELM

2015 ◽  
Vol 738-739 ◽  
pp. 598-601
Author(s):  
Han Yang Zhu ◽  
Xin Yu Jin ◽  
Jian Feng Shen
Keyword(s):  
Computational Complexity ◽  
Prediction Model ◽  
Image Coding ◽  
Region Of Interest ◽  
Image Encoding ◽  
Large Size ◽  
Learning Machine ◽  
Intractable Problem ◽  
Encoding Efficiency ◽  
Better Than

In telemedicine, medical images are always considered very important telemedicine diagnostic evidences. High transmission delay in a bandwidth limited network becomes an intractable problem because of its large size. It’s important to achieve a quality balance between Region of Interest (ROI) and Background Region (BR) when ROI-based image encoding is being used. In this paper, a research made on balancing method of LS-SVM based ROI/BR PSNR prediction model to optimize the ROI encoding shows it’s much better than conventional methods but with very high computational complexity. We propose a new method using extreme learning machine (ELM) with lower computational complexity to improve encoding efficiency compared to LS-SVM based model. Besides, it also achieves the same effect of balancing ROI and BR.

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