Pre-Existing Cross-Reactive Antibody Responses Do Not Significantly Impact Inactivated COVID-19 Vaccine-Induced Neutralization

Recent exposure to seasonal coronaviruses (sCoVs) may stimulate cross-reactive antibody responses against severe acute respiratory syndrome CoV 2 (SARS-CoV-2). However, previous studies have produced divergent results regarding protective or damaging immunity induced by prior sCoV exposure. It remains unknown whether pre-existing humoral immunity plays a role in vaccine-induced neutralization and antibody responses. In this study, we collected 36 paired sera samples from 36 healthy volunteers before and after immunization with inactivated whole-virion SARS-CoV-2 vaccines for COVID-19, and analyzed the distribution and intensity of pre-existing antibody responses at the epitope level pre-vaccination as well as the relationship between pre-existing sCoV immunity and vaccine-induced neutralization. We observed large amounts of pre-existing cross-reactive antibodies in the conserved regions among sCoVs, especially the S2 subunit. Excep t for a few peptides, the IgG and IgM fluorescence intensities against S, M and N peptides did not differ significantly between pre-vaccination and post-vaccination sera of vaccinees who developed a neutralization inhibition rate (%inhibition) <40 and %inhibition ≥40 after two doses of the COVID-19 vaccine. Participants with strong and weak pre-existing cross-reactive antibodies (strong pre-CRA; weak pre-CRA) had similar %inhibition pre-vaccination (10.9% ± 2.9% vs. 12.0% ± 2.2%, P=0.990) and post-vaccination (43.8% ± 25.1% vs. 44.6% ± 21.5%, P=0.997). Overall, the strong pre-CRA group did not show a significantly greater increase in antibody responses to the S protein linear peptides post-vaccination compared with the weak pre-CRA group. Therefore, we found no evidence for a significant impact of pre-existing antibody responses on inactivated vaccine-induced neutralization and antibody responses. Our research provides an important basis for inactivated SARS-CoV-2 vaccine use in the context of high sCoV seroprevalence.

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Systemic and local antibodies induced by an experimental inactivated vaccine against bovine herpesvirus type 1

Ciência Rural ◽

10.1590/s0103-84782011000200021 ◽

2011 ◽

Vol 41 (2) ◽

pp. 307-313

Author(s):

Maria do Carmo Cilento ◽

Edviges Maristela Pituco ◽

Ricardo Spacagna Jordão ◽

Cláudia Pestana Ribeiro ◽

Moacir Marchiori Filho ◽

...

Keyword(s):

Bovine Herpesvirus ◽

Neutralization Assay ◽

Elisa Test ◽

Antibody Responses ◽

Inactivated Vaccine ◽

Serum Samples ◽

Post Vaccination ◽

Herpesvirus Type ◽

Virus Neutralization Assay ◽

Igg2 Antibody

An experimental inactivated vaccine against bovine herpesvirus-1 (BoHV-1) was produced aiming to evaluate the systemic and local antibody responses in 12 seronegative heifers, after vaccination and revaccination. Serum samples were submitted to virus neutralization assay and to ELISA test for detection of IgG1 and IgG2 isotypes. Nasal secretion samples were submitted to the same ELISA test for detection of IgG1 and IgG2 isotypes. The results showed that moderate to high neutralizing titres and IgG1 and IgG2 antibody responses were induced after the second vaccination in the serum and in nasal secretions up to 114 days post vaccination. IgG2 antibodies were the prevalent isotype for most of the post-vaccination period. The results indicate that BoHV-1 experimental inactivated vaccine elicited potentially protective IgG1 and IgG2 antibody levels, both in the systemic and mucosal compartments.

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Robust Antibody Responses to the BNT162b2 mRNA Vaccine Occur Within a Week After the First Dose in Previously Infected Individuals and After the Second Dose in Uninfected Individuals

Frontiers in Immunology ◽

10.3389/fimmu.2021.722766 ◽

2021 ◽

Vol 12 ◽

Cited By ~ 4

Author(s):

Yosuke Hirotsu ◽

Kenji Amemiya ◽

Hiroki Sugiura ◽

Miyuki Shinohara ◽

Mika Takatori ◽

...

Keyword(s):

Health Care Workers ◽

Protein A ◽

Antibody Responses ◽

Post Vaccination ◽

Severity Of Disease ◽

Care Workers ◽

Before And After ◽

Antibody Titres ◽

Kinetics Of ◽

Hourly Basis

BackgroundVaccines against severe acute respiratory syndrome coronavirus 2 can trigger acquired immunity in infection-naïve individuals and offer a path toward ending the coronavirus disease pandemic that began in 2019. However, the kinetics of early antibody responses in vaccinated individuals remain poorly understood.MethodWe followed BNT162b2 mRNA-vaccinated health care workers (HCWs, N=108) including 103 infection-naïve and five previously infected individuals. A total of 763 blood samples were collected weekly or hourly basis before and after vaccination. Serological analysis of anti-spike and anti-nucleocapsid antibodies was performed.ResultsSeroconversion occurred in all infection-naïve HCWs 3 weeks after the first dose (just before the second vaccination) and a marked boosting effect was observed at 4 weeks (1 week after the second dose). Among previously infected HCWs with pre-existing antibodies against the spike protein, a remarkable boosting effect was observed during the first week after vaccination, and a further increase in antibody titres was observed after the second dose. In one previously infected patient, daily blood sampling was conducted. Antibody titres began to increase 96 hours (4 days) after the first dose.ConclusionThe BNT162b2 mRNA vaccine remarkably enhanced antibody responses after the second dose in infection-naïve individuals and after the first dose in previously infected HCWs of all ages and genders. Antibody titres decreased slightly after the 5th week post-vaccination. The robust boosting effect of immunisation suggests that increased antibody titres following exposure to the virus may restrict viral replication, prolong the incubation period, or lessen the severity of disease.

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Enhancing Spatial Ability Through Sport Practice

Journal of Individual Differences ◽

10.1027/1614-0001/a000075 ◽

2012 ◽

Vol 33 (2) ◽

pp. 83-88 ◽

Cited By ~ 58

Author(s):

David Moreau ◽

Jérome Clerc ◽

Annie Mansy-Dannay ◽

Alain Guerrien

Keyword(s):

Mental Rotation ◽

Spatial Representations ◽

Mental Rotation Test ◽

Sport Training ◽

Sport Practice ◽

Undergraduate University Students ◽

Before And After ◽

Cognitive Plasticity ◽

Reported Data ◽

The Relationship

This experiment investigated the relationship between mental rotation and sport training. Undergraduate university students (n = 62) completed the Mental Rotation Test ( Vandenberg & Kuse, 1978 ), before and after a 10-month training in two different sports, which either involved extensive mental rotation ability (wrestling group) or did not (running group). Both groups showed comparable results in the pretest, but the wrestling group outperformed the running group in the posttest. As expected from previous studies, males outperformed women in the pretest and the posttest. Besides, self-reported data gathered after both sessions indicated an increase in adaptive strategies following training in wrestling, but not subsequent to training in running. These findings demonstrate the significant effect of training in particular sports on mental rotation performance, thus showing consistency with the notion of cognitive plasticity induced from motor training involving manipulation of spatial representations. They are discussed within an embodied cognition framework.

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Klein with Lacan: A Study on the Reception of Lacanian Ideas in Uruguay and Its Effects on Clinical Practices (1955–82)

Psychoanalysis and History ◽

10.3366/pah.2020.0353 ◽

2020 ◽

Vol 22 (3) ◽

pp. 341-361

Author(s):

Gonzalo Grau-Pérez ◽

J. Guillermo Milán

Keyword(s):

Clinical Practice ◽

Clinical Material ◽

Clinical Practices ◽

Before And After ◽

The Difference ◽

The 1960S ◽

The Relationship ◽

Discursive Approach ◽

The Way ◽

Clinical Cases

In Uruguay, Lacanian ideas arrived in the 1960s, into a context of Kleinian hegemony. Adopting a discursive approach, this study researched the initial reception of these ideas and its effects on clinical practices. We gathered a corpus of discursive data from clinical cases and theoretical-doctrinal articles (from the 1960s, 1970s and 1980s). In order to examine the effects of Lacanian ideas, we analysed the difference in the way of interpreting the clinical material before and after Lacan's reception. The results of this research illuminate some epistemological problems of psychoanalysis, especially the relationship between theory and clinical practice.

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STUDY ON THE RELATIONSHIP BETWEEN PARASITIC INFECTION AND KNOWLEDGE, HYGIENIC PRACTICAL MEASURES OF PARASITIC INFECTIOUS PREVENTION IN VINH THAI COMMUNITY

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2017.4.18 ◽

2017 ◽

pp. 119-124

Author(s):

Keyword(s):

Health Education ◽

Risk Behaviors ◽

Ascaris Lumbricoides ◽

Intestinal Parasite ◽

Parasitic Infection ◽

Parasite Infection ◽

Intestinal Parasite Infection ◽

Before And After ◽

Parasite Infections ◽

The Relationship

Introduction: Intestinal parasite infections werecommonintropical country such as Vietnam. Having good knowledge of parasitic infectious prevention and changing risk behaviors can decrease the infection rate. Objective: To evaluate the parasitic infectious rate in Vinh Thai community before and after being health education and the changing of knowledge of parasitic infectious prevention and risk behaviors. Materials and methods: 60 households in Vinh Thai commune were interviewed their knowledge of parasitic infectious prevention and examined intestinal parasite infection by Kato technique and then trained the knowledge of parasitic infectious prevention. The interview and examination parasite infectiousrate were carried out after 6 months to evaluating their knowledge. Result: Before health education, the rate of intestinal parasite infection was 17.4% with the prevalence of Ascaris lumbricoides, hookworm, whipworm, pinworm, small fluke worm and co-infection with A. lumbricoides - whipworm, hookworm-whipworm were 0.1%; 8.0%; 5.8%; 0.6%; 0.3%; 1.2% and 3.0% respectively. Six months later the rate of intestinal parasite infection was decreased in 12.6% even though not statistical significantly. However, there were no case of small fluke worm and co-infection with hookworm-whipworm. Receiving health education, their knowledge of parasitic infectious prevention was higher significantly but their risk behaviors were not changed so much. Conclusion: Health education can change the rate of parasite infection with higher knowledge of parasitic infectious prevention but it was necessary continuous study to change the risk behaviors. Key words: intestinal parasite, health education

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Sunlight exposure: the effects on the performance of paragliding fabric

Industria Textila ◽

10.35530/it.069.05.1406 ◽

2018 ◽

Vol 69 (05) ◽

pp. 381-389

Author(s):

MENGÜÇ GAMZE SÜPÜREN ◽

TEMEL EMRAH ◽

BOZDOĞAN FARUK

Keyword(s):

Mechanical Properties ◽

Aging Process ◽

Air Permeability ◽

Sunlight Exposure ◽

Test Results ◽

Coating Materials ◽

Strength Tests ◽

Before And After ◽

The Relationship ◽

Dark Blue

This study was designed to explore the relationship between sunlight exposure and the mechanical properties of paragliding fabrics which have different colors, densities, yarn counts, and coating materials. This study exposed 5 different colors of paragliding fabrics (red, turquoise, dark blue, orange, and white) to intense sunlight for 150 hours during the summer from 9:00 a.m. to 3:00 p.m. for 5 days a week for 5 weeks. Before and after the UV radiation aging process, the air permeability, tensile strength, tear strength, and bursting strength tests were performed. Test results were also evaluated using statistical methods. According to the results, the fading of the turquoise fabric was found to be the highest among the studied fabrics. It was determined that there is a significant decrease in the mechanical properties of the fabrics after sunlight exposure. After aging, the fabrics become considerably weaker in the case of mechanical properties due to the degradation in both the dyestuff and macromolecular structure of the fiber

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APPLICATION OF THE ATP-BIOLUMINESCENCE METHOD TO ASSESS THE QUALITY OF SANITATION OF MILKING EQUIPMENT

Problems of Veterinary Sanitation, Hygiene and Ecology ◽

10.36871/vet.san.hyg.ecol.202004010 ◽

2020 ◽

Vol 1 (4) ◽

pp. 468-472

Author(s):

P.A. Popov ◽

◽

D.V. Gruznov ◽

S.V. Tokarev ◽

◽

...

Keyword(s):

Dairy Farms ◽

Laboratory Studies ◽

Before And After ◽

Atp Bioluminescence ◽

Microbial Number ◽

Proportional Relationship ◽

The Relationship ◽

Number Of Bacteria ◽

Bioluminescence Method

The article presents the results of determining the relationship between the total microbial number and microbial ATP on the surface of milking equipment before and after treatment with neutral anolyte ANK-SUPER. The possibility of using the ATP-bioluminescence method to control the quality of sanitation of milking equipment on dairy farms is shown. Laboratory studies revealed a proportional relationship between the level of bacterial ATP and the number of bacteria on the surface of milking equipment before and after sanitation.

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Post-Drive Standing Balance of Vehicle Passengers Using Wearable Sensors: The Effect of On-Road Driving and Task Performance

Sensors ◽

10.3390/s21154997 ◽

2021 ◽

Vol 21 (15) ◽

pp. 4997

Author(s):

Victor C. Le ◽

Monica L. H. Jones ◽

Kathleen H. Sienko

Keyword(s):

Task Performance ◽

Postural Sway ◽

Wearable Sensors ◽

Standing Balance ◽

Balance Performance ◽

Real Time Traffic ◽

Balance Exercises ◽

Before And After ◽

The Relationship ◽

And Task

Postural sway has been demonstrated to increase following exposure to different types of motion. However, limited prior studies have investigated the relationship between exposure to normative on-road driving conditions and standing balance following the exposure. The purpose of this on-road study was to quantify the effect of vehicle motion and task performance on passengers’ post-drive standing balance performance. In this study, trunk-based kinematic data were captured while participants performed a series of balance exercises before and after an on-road driving session in real-time traffic. Postural sway for all balance exercises increased following the driving session. Performing a series of ecologically relevant visual-based tasks led to increases in most post-drive balance metrics such as sway position and velocity. However, the post-drive changes following the driving session with a task were not significantly different compared to changes observed following the driving session without a task. The post-drive standing balance performance changes observed in this study may increase vulnerable users’ risk of falling. Wearable sensors offer an opportunity to monitor postural sway following in-vehicle exposures.

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Reduction in mobility and COVID-19 transmission

Nature Communications ◽

10.1038/s41467-021-21358-2 ◽

2021 ◽

Vol 12 (1) ◽

Cited By ~ 7

Author(s):

Pierre Nouvellet ◽

Sangeeta Bhatia ◽

Anne Cori ◽

Kylie E. C. Ainslie ◽

Marc Baguelin ◽

...

Keyword(s):

Predictive Ability ◽

Control Measures ◽

Social Distancing ◽

Beneficial Effects ◽

Mobility Data ◽

Strict Control ◽

Before And After ◽

Proxy Measure ◽

Lower Transmission ◽

The Relationship

AbstractIn response to the COVID-19 pandemic, countries have sought to control SARS-CoV-2 transmission by restricting population movement through social distancing interventions, thus reducing the number of contacts. Mobility data represent an important proxy measure of social distancing, and here, we characterise the relationship between transmission and mobility for 52 countries around the world. Transmission significantly decreased with the initial reduction in mobility in 73% of the countries analysed, but we found evidence of decoupling of transmission and mobility following the relaxation of strict control measures for 80% of countries. For the majority of countries, mobility explained a substantial proportion of the variation in transmissibility (median adjusted R-squared: 48%, interquartile range - IQR - across countries [27–77%]). Where a change in the relationship occurred, predictive ability decreased after the relaxation; from a median adjusted R-squared of 74% (IQR across countries [49–91%]) pre-relaxation, to a median adjusted R-squared of 30% (IQR across countries [12–48%]) post-relaxation. In countries with a clear relationship between mobility and transmission both before and after strict control measures were relaxed, mobility was associated with lower transmission rates after control measures were relaxed indicating that the beneficial effects of ongoing social distancing behaviours were substantial.

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ACE Inhibitory and Antihypertensive Activities of Wine Lees and Relationship among Bioactivity and Phenolic Profile

Nutrients ◽

10.3390/nu13020679 ◽

2021 ◽

Vol 13 (2) ◽

pp. 679

Author(s):

Raúl López-Fernández-Sobrino ◽

Jorge R. Soliz-Rueda ◽

Maria Margalef ◽

Anna Arola-Arnal ◽

Manuel Suárez ◽

...

Keyword(s):

Hypotensive Effect ◽

Wine Industry ◽

Antihypertensive Activity ◽

Spontaneously Hypertensive ◽

Lowering Effect ◽

Before And After ◽

Phenolic Profiles ◽

Wistar Kyoto ◽

Grape Varieties ◽

The Relationship

Wine lees (WL) are by-products generated in the winemaking process. The aim of this study was to investigate the angiotensin-converting enzyme inhibitory (ACEi) activity, and the blood pressure (BP) lowering effect of WL from individual grape varieties. The relationship among their activities and phenolic profiles was also studied. Three WL, from Cabernet, Mazuela, and Garnacha grape varieties, were firstly selected based on their ACEi properties. Their phenolic profiles were fully characterized by UHPLC-ESI-Q-TOF-MS. Then, their potential antihypertensive effects were evaluated in spontaneously hypertensive rats (SHR). BP was recorded before and after their oral administrations (2, 4, 6, 8, 24, and 48 h) at a dose of 5 mL/kg bw. Cabernet WL (CWL) exhibited a potent antihypertensive activity, similar to that obtained with the drug Captopril. This BP-lowering effect was related to the high amount of anthocyanins and flavanols present in these lees. In addition, a potential hypotensive effect of CWL was discarded in normotensive Wistar–Kyoto rats. Finally, the ACEi and antihypertensive activities of CWL coming from a different harvest were confirmed. Our results suggest the potential of CWL for controlling arterial BP, opening the door to commercial use within the wine industry.

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