scholarly journals Pollen Streptomyces Produce Antibiotic That Inhibits the Honey Bee Pathogen Paenibacillus larvae

2021 ◽  
Vol 12 ◽  
Author(s):  
Kirk J. Grubbs ◽  
Daniel S. May ◽  
Joseph A. Sardina ◽  
Renee K. Dermenjian ◽  
Thomas P. Wyche ◽  
...  
Keyword(s):  
Natural Products ◽  
Honey Bees ◽  
Inhibitory Activity ◽  
Critical Role ◽  
Paenibacillus Larvae ◽  
Modern Agriculture ◽  
Antibiotic Compounds ◽  
Pathogenic Microbes ◽  
Hplc Fractionation ◽  
Potent Inhibitory Activity

Humans use natural products to treat disease; similarly, some insects use natural products produced by Actinobacteria to combat infectious pathogens. Honey bees, Apis mellifera, are ecologically and economically important for their critical role as plant pollinators and are host to diverse and potentially virulent pathogens that threaten hive health. Here, we provide evidence that Actinobacteria that can suppress pathogenic microbes are associated with A. mellifera. We show through culture-dependent approaches that Actinobacteria in the genus Streptomyces are commonly isolated from foraging bees, and especially common in pollen stores. One strain, isolated from pollen stores, exhibited pronounced inhibitory activity against Paenibacillus larvae, the causative agent of American foulbrood. Bioassay-guided HPLC fractionation, followed by NMR and mass spectrometry, identified the known macrocyclic polyene lactam, piceamycin that was responsible for this activity. Further, we show that in its purified form, piceamycin has potent inhibitory activity toward P. larvae. Our results suggest that honey bees may use pollen-derived Actinobacteria and their associated small molecules to mediate colony health. Given the importance of honey bees to modern agriculture and their heightened susceptibility to disease, the discovery and development of antibiotic compounds from hives could serve as an important strategy in supporting disease management within apiaries.

scholarly journals Testing of inhibition activity of essential oils against Paenibacillus larvae – the causative agent of American foulbrood

2014 ◽  
Vol 83 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Katarína Kuzyšinová ◽  
Dagmar Mudroňová ◽  
Juraj Toporčák ◽  
Radomíra Nemcová ◽  
Ladislav Molnár ◽  
...  
Keyword(s):  
Essential Oils ◽  
Honey Bees ◽  
Inhibitory Activity ◽  
Paenibacillus Larvae ◽  
Syzygium Aromaticum ◽  
American Foulbrood ◽  
Inhibition Activity ◽  
Foeniculum Vulgare ◽  
Plant Essential Oils ◽  
Carum Carvi

American foulbrood is a dangerous world-wide spread disease of honey bees caused by the Paenibacillus larvae bacterium. Antibiotic treatments are less effective and leave residues in bee products. It is therefore necessary to find an alternative, especially using natural ingredients such as plant essential oils, probiotics, fatty or organic acids. Two strains of P. larvae were used for this study: CCM 4488, a strain from the Czech collection of micro-organisms and a Slovak field strain which was isolated from infected bee combs and characterized on the basis of biochemical properties. Plant essential oils of sage (Salvia officinalis), anise (Pimpinella anisum), oregano (Origanum vulgare), caraway (Carum carvi), thyme (Thymus vulgaris), rosemary (Rosmarinum officinalis), clove (Syzygium aromaticum), camomile (Chamomilla recutita) and fennel (Foeniculum vulgare) were used for the testing of the inhibitory activity against P. larvae. Essential oils at amounts of 5 µl and 10 µl were applied to sterile discs on MYPGP agar; inhibition zone diameters were measured after 24-h incubation at 37 °C. The strongest inhibitory activity against both P. larvae strains was noted in case of the essential oils from oregano, thyme and clove; essential oils from camomile, rosemary and fennel showed no or weak antibacterial activity. Medium strong inhibition activity was recorded in case of previously untested essential oil from Carum carvi. There was a difference in sensitivity of both tested strains to essential oils. Our study confirmed that some essential oils can be used in the prevention of American foulbrood but further experiments aimed at their influence on physiological intestinal microflora of honey bees must be performed.

scholarly journals Massiliamide, a cyclic tetrapeptide with potent tyrosinase inhibitory properties from the Gram-negative bacterium Massilia albidiflava DSM 17472T

2020 ◽  
Author(s):  
Andri Frediansyah ◽  
Jan Straetener ◽  
Heike Brötz-Oesterhelt ◽  
Harald Gross
Keyword(s):  
Absolute Configuration ◽  
Inhibitory Activity ◽  
Liquid Culture ◽  
Spectroscopic Analysis ◽  
2D Nmr ◽  
Negative Bacterium ◽  
Gram Negative ◽  
The Absolute ◽  
Potent Inhibitory Activity ◽  
Cyclic Tetrapeptide

AbstractA cyclic tetrapeptide, designated massiliamide, was isolated from the liquid culture of the Gram-negative bacterium Massilia albidiflava DSM 17472T. The structure was elucidated through extensive spectroscopic analysis, including HR-MS and 1D and 2D NMR experiments. The absolute configuration was determined using the Marfey´s method. Massiliamide showed potent inhibitory activity towards tyrosinase with an IC50 value of 1.15 µM and no cytotoxicity.

scholarly journals Antiglycation potential of Indigoferin a, Indigoferin B and Indigoferin C natural products from Indigofera heterantha Brandis

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Ayesha Khan ◽  
Ajmal Khan ◽  
Manzoor Ahmad ◽  
Mumtaz Ali ◽  
Umar Farooq ◽  
...  
Keyword(s):  
Natural Products ◽  
Diabetic Complications ◽  
Inhibitory Activity ◽  
Chemical Constituents ◽  
Intramolecular Hydrogen Bonding ◽  
Glycation End Products ◽  
End Products ◽  
Serious Disease ◽  
Intramolecular Hydrogen

Abstract Background Diabetes is a long-lasting and serious disease that effect in worldwide individual lives, families, and societies. Hyperglycemia of diabetes mellitus produced Advance Glycation End Products that are associated with diabetic complications like neuropathy, nephropathy, retinopathy, and cardiovascular diseases. Methods In this study, the natural products isolated from of Indigofera heterantha Brandis, Indigoferin A (S1), Indigoferin B (S2) and Indigoferin C (S3) were evaluated for their in vitro antiglycation activity. Results The compounds exhibited a significant inhibitory activity against the formation of Advanced Glycation End-Products with IC50 values of 674.25 ± 3.2 μM, 407.03 ± 4.7 μM and 726.41 ± 2.1 μM, respectively. Here, important structure-activity relationship was observed, when the intramolecular hydrogen bonding interactions suppressed the antiglycation activity of compound S3. Thus, the study clearly demonstrates that the number and the position of substituents act as an assisting factor and directly influence the inhibitory activity of the natural product by altering the sugar or protein binding affinity. Conclusions This study explain first time the antiglycation inhibitory ability of chemical constituents isolated from I. heterantha and can be used for above late diabetic complications.

scholarly journals New Mammalian Target of Rapamycin (mTOR) Modulators Derived from Natural Product Databases and Marine Extracts by Using Molecular Docking Techniques

Marine Drugs ◽  
2018 ◽  
Vol 16 (10) ◽  
pp. 385 ◽  
Author(s):  
Verónica Ruiz-Torres ◽  
Maria Losada-Echeberría ◽  
Maria Herranz-López ◽  
Enrique Barrajón-Catalán ◽  
Vicente Galiano ◽  
...  
Keyword(s):  
Natural Products ◽  
Molecular Docking ◽  
Side Effects ◽  
Inhibitory Activity ◽  
Mammalian Target Of Rapamycin ◽  
Therapeutic Use ◽  
Cellular Growth ◽  
Target Of Rapamycin ◽  
Computational Techniques ◽  
Chemical Derivatives

Mammalian target of rapamycin (mTOR) is a PI3K-related serine/threonine protein kinase that functions as a master regulator of cellular growth and metabolism, in response to nutrient and hormonal stimuli. mTOR functions in two distinct complexes—mTORC1 is sensitive to rapamycin, while, mTORC2 is insensitive to this drug. Deregulation of mTOR’s enzymatic activity has roles in cancer, obesity, and aging. Rapamycin and its chemical derivatives are the only drugs that inhibit the hyperactivity of mTOR, but numerous side effects have been described due to its therapeutic use. The purpose of this study was to identify new compounds of natural origin that can lead to drugs with fewer side effects. We have used computational techniques (molecular docking and calculated ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) parameters) that have enabled the selection of candidate compounds, derived from marine natural products, SuperNatural II, and ZINC natural products, for inhibitors targeting, both, the ATP and the rapamycin binding sites of mTOR. We have shown experimental evidence of the inhibitory activity of eleven selected compounds against mTOR. We have also discovered the inhibitory activity of a new marine extract against this enzyme. The results have been discussed concerning the necessity to identify new molecules for therapeutic use, especially against aging, and with fewer side effects.

Synthesis and antibacterial evaluation of novel Schiff's base derivatives of nitroimidazole nuclei as potent E. coli FabH inhibitors

RSC Advances ◽  
2014 ◽  
Vol 4 (97) ◽  
pp. 54217-54225 ◽  
Author(s):  
Xin Zhang ◽  
Chetan B. Sangani ◽  
Li-Xin Jia ◽  
Pi-Xian Gong ◽  
Fang Wang ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Schiff’S Base ◽  
E Coli ◽  
Derivatives Of ◽  
Antibacterial Evaluation ◽  
Potent Inhibitory Activity

Series of novel Schiff's base derivatives have been synthesized. Compound 10q showed the most potent inhibitory activity (IC50 = 2.6883 μM).

scholarly journals Antimicrobial activity of fifteen Italian honeys against Paenibacillus larvae ATCC 9545

2018 ◽  
Vol 68 (4) ◽  
pp. 547 ◽  
Author(s):  
S. SAGONA ◽  
B. TURCHI ◽  
F. FRATINI ◽  
M. GIUSTI ◽  
B. TORRACCA ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Glucose Oxidase ◽  
Inhibitory Activity ◽  
Oxidase Activity ◽  
Gluconic Acid ◽  
Total Flavonoid ◽  
Paenibacillus Larvae ◽  
Economic Losses ◽  
Total Phenolic ◽  
Honey Sample

Currently, American Foulbrood (AFB) represents one of the most important problems for beekeepers, due to economic losses and to the absence of an effective therapeutic treatment. The aim of this work was to characterize fifteen Italian honeys in order to assess their inhibitory activity against Paenibacillus larvae ATCC 9545. Each honey was analyzed for the activity of the following enzymes: glucose oxidase and catalase. Moreover, melissopalynological analysis and other biochemical parameters, namely gluconic acid, total phenolic and total flavonoid contents were determined. For each honey, the Minimum Inhibitory Concentration (M.I.C.) and the Minimum Bactericidal Concentration (M.B.C.) against P. larvae were determined. All tested honey samples had an inhibitory activity on P. larvae. In particular, the lowest M.I.C. and M.B.C. values (53.8 mg/mL and 107.5 mg/mL, respectively) were recorded for an Arbutus honey sample. Arbutus honeys also had the highest gluconic acid and total phenolic contents (12.6 ± 1.7 g/kg and 243.2 ± 25.1 mg/kg, respectively) and the highest glucose oxidase activity (13.0 ± 1.9 nM H2O2/min). Dark honeys, including Arbutus, seem to have a higher gluconic acid content and a higher antimicrobial activity. Thus, honey characterization, including colour and physico-chemical characteristics (e.g. gluconic acid concentration, total phenolic and total flavonoid contents, glucose oxidase activity), could be crucial for the assessment of its employment against P. larvae.

scholarly journals Natural Products can Efficiently Control the Greater Wax Moth (Lepidoptera: Pyralidae), but are Harmless to Honey Bees

Sociobiology ◽  
2020 ◽  
Vol 67 (1) ◽  
pp. 89
Author(s):  
Daniele Maria Telles ◽  
Gabriel Moreno Martineli ◽  
Maurice Fabian Scaloppi ◽  
Marina Pagliai Ferreira Luz ◽  
Samir Moura Kadri ◽  
...  
Keyword(s):  
Natural Products ◽  
Population Growth ◽  
Honey Bees ◽  
Galleria Mellonella ◽  
Greater Wax Moth ◽  
Lepidoptera Pyralidae ◽  
Tobacco Extract ◽  
Bee Colonies ◽  
Wax Moth

Honey bees (Apis mellifera L.) have great global socioeconomic and environmental importance. However, the greater wax moth (Galleria mellonella L.) is a pest that causes serious worldwide damage to honey bee colonies. Good beekeeping practices and physical, chemical, or natural methods can be used to control wax moths. The use of natural products is a more sustainable option because of their lower toxicity to the environment and the colony. Therefore, we evaluated the efficiency of four natural products for greater wax moth control: neem oil (Azadirachta indica), eucalyptus oil (Eucalyptus spp.), tobacco extract (Nicotiana tabacum), and malagueta pepper extract (Capsicum frutescens). We also evaluated their effects on adult bees and on the population growth of colonies. The 4th instar wax moths and adult bees were subjected to in vitro bioassays of different concentrations of the products. The results allowed usto establish a concentration for each product that was safe for the bees and effectively controlled the moth. Then, we sprayed them on bee colonies to evaluate their effects on population growth. The neem and eucalyptus oils caused wax moth mortality at low concentrations, but did not affect colony population growth. However, they did have a toxic effect on adult bees. The tobacco and pepper extracts efficiently controlled the moth, but did not cause adult bee mortality or interfered with the population growth of the colonies. Therefore, the tobacco and pepper extracts could efficiently control the greater wax moth, without damaging honey bees.

The Role of Aromatase Enzyme in Hormone Related Diseases and Plant-Based Aromatase Inhibitors as Therapeutic Regimens

2021 ◽  
Vol 21 ◽  
Author(s):  
Kevser Taban Akça ◽  
Murside Ayşe Demirel ◽  
Ipek Süntar
Keyword(s):  
Natural Products ◽  
Aromatase Inhibitors ◽  
Inhibitory Activity ◽  
Chemical Compounds ◽  
Synthesis Methods ◽  
Herbal Drugs ◽  
Drug Discovery And Development ◽  
History Of ◽  
History Of Use

: Medicinal plants have a long history of use as food and remedy in traditional and modern societies, as well as have been used as herbal drugs and sources of novel bioactive compounds. They provide a wide array of chemical compounds, many of which can not be synthesized via current synthesis methods. Natural products may provide aromatase inhibitory activity through various pathways and may act clinically effective for treating pathologies associated with excessive aromatase secretion including breast, ovarian and endometrial cancers, endometriosis, uterine fibroid, benign prostatic hyperplasia (BPH), prostate cancer, infertility, and gynecomastia. Recent studies have shown that natural products with aromatase inhibitory activity, could also be good options against secondary recurrence of breast cancer by exhibiting chemopreventive effects. Therefore, screening for new plant-based aromatase inhibitors may provide novel leads for drug discovery and development, particularly with increased clinical efficacy and decreased side effects.

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