scholarly journals Multi-Omic Analysis of Symbiotic Bacteria Associated With Aedes aegypti Breeding Sites

2021 ◽  
Vol 12 ◽  
Author(s):  
Katherine D. Mosquera ◽  
Luis E. Martinez Villegas ◽  
Sacha J. Pidot ◽  
Chinhda Sharif ◽  
Sven Klimpel ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Water Samples ◽  
Gravid Female ◽  
Breeding Site ◽  
Gene Clusters ◽  
Symbiotic Bacteria ◽  
Potential Contribution ◽  
Breeding Sites ◽  
Mosquito Breeding

Mosquito breeding sites are complex aquatic environments with wide microbial diversity and physicochemical parameters that can change over time during the development of immature insect stages. Changes in biotic and abiotic conditions in water can alter life-history traits of adult mosquitos but this area remains understudied. Here, using microbial genomic and metabolomics analyses, we explored the metabolites associated with Aedes aegypti breeding sites as well as the potential contribution of Klebsiella sp., symbiotic bacteria highly associated with mosquitoes. We sought to address whether breeding sites have a signature metabolic profile and understand the metabolite contribution of the bacteria in the aquatic niches where Ae. aegypti larvae develop. An analysis of 32 mosquito-associated bacterial genomes, including Klebsiella, allowed us to identify gene clusters involved in primary metabolic pathways. From them, we inferred metabolites that could impact larval development (e.g., spermidine), as well as influence the quality assessment of a breeding site by a gravid female (e.g., putrescine), if produced by bacteria in the water. We also detected significant variance in metabolite presence profiles between water samples representing a decoupled oviposition event (oviposition by single females and manually deposited eggs) versus a control where no mosquito interactions occurred (PERMANOVA: p < 0.05; R2 = 24.64% and R2 = 30.07%). Five Klebsiella metabolites were exclusively linked to water samples where oviposition and development occurred. These data suggest metabolomics can be applied to identify compounds potentially used by female Ae. aegypti to evaluate the quality of a breeding site. Elucidating the physiological mechanisms by which the females could integrate these sensory cues while ovipositing constitutes a growing field of interest, which could benefit from a more depurated list of candidate molecules.

THE MATING PATTERNS OF FEMALES WHEN TERRITORIAL DIFFERENCES AMONG MALES ARE REDUCED: A TEST IN THE POLYGYNOUS BEAUGREGORY DAMSELFISH

Behaviour ◽  
2001 ◽  
Vol 138 (6) ◽  
pp. 691-708 ◽  
Author(s):  
◽  
◽  
◽  
Keyword(s):  
Random Mating ◽  
Breeding Site ◽  
Male Reproduction ◽  
Male Reproductive Success ◽  
Mating Pattern ◽  
Breeding Sites ◽  
Site Structure ◽  
Artificial Breeding ◽  
Stegastes Leucostictus

AbstractUsing the Caribbean beaugregory damselfish (Stegastes leucostictus) we tested whether patterns of male reproduction could be modified by reducing differences among males, in this case by giving males identical artificial breeding sites. Previous studies have shown that very few males using the natural small rubble habitat reproduced and variations in male reproductive success were based mainly on the differences in the quality of their natural breeding sites. By providing males with identical artificial breeding sites, we tested whether females would be less likely to confine their reproduction to only a few of the available males. We examined male reproductive patterns by first simulating females choosing males (1) 'randomly,' (i.e. the number of males that receive eggs is based on each female randomly choosing a mate from a group of males), or (2) 'exclusively' (i.e. only one female mates with one male on a given day), or (3) 'highly selectively' (i.e. this is the typical polygynous pattern in which many females select the same few males). We tested these simulations against the actual daily amounts of eggs deposited and the number of different males that received eggs in each of 4 summers. Although the regression trend line from the daily patterns did resemble the Random Mating Pattern, egg clutches were more widely dispersed than random but less dispersed than the Exclusive Mating Pattern. That is, with more females mating on a particular day, more males received eggs. We speculated that this might have resulted from females aggressively excluding other females from mating with the same male on a given day. In contrast, males using the variable quality natural sites rarely mated and those that did receive eggs, received them in larger amounts than males using the artificial sites. Thus, for natural sites, more females seemed to be mating with the same few males. Perhaps for these natural sites any intra-female aggression may have been ineffective when so few superior spawning sites were available. For males using the artificial sites, the total amount of eggs received over a two-month period was nonrandom and resembled the 'highly selected pattern.' This resulted from some males receiving egg clutches on more days. Other traits, besides breeding site structure, may have been important in causing different females to select the same males on subsequent days.

Building multi‐layered resilience in a malaria control programme in Dar es Salaam, Tanzania

2010 ◽  
Vol 10 (4) ◽  
pp. 309-324 ◽  
Author(s):  
Stefan Dongus ◽  
Constanze Pfeiffer ◽  
Emmy Metta ◽  
Selemani Mbuyita ◽  
Brigit Obrist
Keyword(s):  
Malaria Control ◽  
Breeding Site ◽  
Control Programme ◽  
Malaria Vectors ◽  
Malaria Control Programme ◽  
Social Resilience ◽  
Breeding Sites ◽  
Mosquito Breeding ◽  
Field Workers ◽  
Local Leaders

This study applied the multi‐layered social resilience framework in the context of an urban malaria control programme by using a qualitative approach. It was found that exchange between and within administrative levels supported resilience‐building processes in terms of mosquito breeding site elimination. ‘Reactive’ and ‘proactive’ capacities were successfully built among programme staff. However, more potential could be tapped among local leaders and household members, by increasing their competence in eliminating breeding sites of malaria vectors. Improving the communication skills of the programme’s field workers might support such processes. Together with local leaders, they could act as multipliers of sensitisation messages.

scholarly journals MAYA INDEX DAN KEPADATAN LARVA AEDES AEGYPTI ANTARA DUSUN TEGALREJO DAN DUSUN KRAJAN KIDUL NANGGUNGAN PACITAN

2019 ◽  
Vol 14 (2) ◽  
pp. 199
Author(s):  
Yelly Atiefsa Narmala ◽  
R. Azizah
Keyword(s):  
Aedes Aegypti ◽  
Dengue Fever ◽  
Public Health Problem ◽  
Simple Random Sampling ◽  
Risk Category ◽  
Cross Sectional ◽  
Breeding Sites ◽  
Mosquito Breeding ◽  
Descriptive Approach ◽  
Cross Sectional Design

Dengue fever remains a public health problem. Environmental factors influence the mosquito Aedes aegypti’s growth, especially if there are many containers in the neighborhood. The community of Nanggungan Village have a habit of storing water in containers, therefore, they risk to become breeding sites for mosquitoes. This study aims to identify the Maya Index status of Aedes aegypti between Tegalrejo and Krajan Kidul Village. The research was observational with a cross-sectional design. Total samples were 200 homes, which 100 homes from Tegalrejo and 100 homes from Krajan Kidul Village, taken by simple random sampling. The measurement of variables employed observation sheet and analyzed in a descriptive approach. The number of containers observed in the Tegalrejo Village was 394 units, and Karajan Kidul Village was 391 units. Maya Index statuses in Tegalrejo (92%) and Krajan Kidul Village (88%) were low. Maya Index status in Krajan Kidul (13%) was higher than Tegalrejo Village (8%). House Index (HI) in the Tegalrejo (18.0%) was lower than Krajan Kidul Village (25.0%), Container Index in Tegalrejo (5.30%) was lower than in Krajan Kidul Village (8.95%), Breteau Index in Tegalrejo (21.0%) was lower than in Krajan Kidul Village (35.0%), Density Figure in Tegalrejo and Krajan Kidul Village indicated a scale of 3 and 4. Based on the MI’s status, Tegalrejo and Krajan Kidul Village were included as a low-risk category of mosquito breeding sites. Based on the density number of larvae, two villages have a moderate risk of Dengue Fever transmission. The community should implement the Mosquitoes Breeding Sites Eradication Program (PSN 3M Plus) and minimize the presence of the containers.

scholarly journals Surveilans Aedes aegypti di Daerah Endemis Demam Berdarah Dengue

2014 ◽  
Vol 8 (8) ◽  
pp. 423
Author(s):  
Sunaryo Sunaryo ◽  
Nova Pramestuti
Keyword(s):  
High Risk ◽  
Aedes Aegypti ◽  
Health Problem ◽  
Haemorrhagic Fever ◽  
Cross Sectional ◽  
Breeding Sites ◽  
Central Java ◽  
Mosquito Breeding ◽  
Survey Results ◽  
Cross Sectional Design

Demam Berdarah Dengue (DBD) perlu mendapat perhatian serius karena masih menjadi masalah kesehatan di Indonesia dan di beberapa daerah masih sering terjadi kejadian luar biasa. Di Jawa Tengah, kasus DBD cenderung meningkat setiap tahunnya terutama pada tahun 2012. Penelitian ini bertujuan menganalisis parameter entomologi dan menggambarkan jenis tempat penampungan air. Penelitian dilakukan di Kabupaten Grobogan, Purbalingga, Kendal dan Kota Semarang pada bulan Juni - Oktober 2013 desain potong lintang. Survei jentik dilakukan untuk melihat keberadaan tempat penampungan air pada 100 rumah. Masing-masing kabupaten dipilih tiga lokasi desa endemis DBD. Hasil survei digunakan untuk menghitung nilai parameter entomologi. Hasil penelitian menunjukkan bahwa meningkatnya kasus DBD di empat kabupaten/kota terkait dengan keberadaan vektor A. aegypti. Hal ini dibuktikan dengan masih tingginya persentase jumlah rumah yang ditemukan jentik A. aegypti (House Index > 10%) serta tingginya jumlah kontainer ditemukan jentik A. aegypti pada rumah yang dilakukan survei (Breteau Index). Nilai ovitrap index paling tinggi di Desa Kalikabong Kabupaten Purbalingga sebesar 40%. Proporsi controllable site lebih banyak daripada disposable site, berarti rumah tersebut berisiko tinggi sebagai tempat perkembangbiakan nyamuk.Dengue Haemorrhagic Fever (DHF) needs serious attention because it is still a health problem in Indonesia and in recent area DHF caused outbreak. In Central Java, incidence of DHF high every years, especially in 2012. This study aimed to analyze the parameters of entomology and describe types of containers. The study was conducted in Grobogan, Purbalingga, Kendal District and Semarang City in June _ October 2013 with cross-sectional design. Larvae survey had been done in 100 houses in three villages that endemic DHF at every district/city. The survey results are used to calculate parameter entomology. The results showed that existance of DHF cases in four district/city connected with the population of A. aegypti. This matter proved with high percentage of houses that found A. aegypti (House Index > 10%) and the high of container that containing A. aegypti in every houses (Breteau Index). The high of ovitrap index (OI) was 40% in Kalikabong village, Purbalingga district. The proportion of controllable sites more than disposable sites, meaning the house as the high risk of mosquito breeding sites. 

The biting flies of the Kano Plains, Kenya: Part II. Larval habitats of common mosquito species (Dipt., Culicidae)

1971 ◽  
Vol 61 (2) ◽  
pp. 299-307 ◽  
Author(s):  
Canute P. M. Khamala
Keyword(s):  
Culex Pipiens ◽  
Mosquito Species ◽  
Breeding Site ◽  
Breeding Sites ◽  
Larval Habitats ◽  
Emergent Plants ◽  
Culex Pipiens Fatigans ◽  
Breeding Habitats ◽  
Mosquito Breeding ◽  
Selection Of

Six natural terrestrial mosquito breeding habitats were recognised in the Kano Plains of Kenya by their condition, i.e. temporary or permanent, presence or absence of emergent plants, and by the chemical and physical characteristics of their water. Between September 1968 and March 1970, 13 mosquito species were found breeding in these habitats. Most species were restricted to a few habitats; only four showed a wide occurrence, being found in at least four. Anopheles gambiae Giles and Culex annulioris Theo. exploited all six habitats, suggesting that the characteristics studied were probably not critical to their selection of a breeding site. Mansonia and Coquillettidia spp. were restricted to habitats with some plants, indicating their dependence on such vegetation for respiration and protection. The indiscriminate selection of breeding sites by disease vectors, e.g. A. gambiae, A. pharoensis Theo. and Culex pipiens fatigans Wied., is worth noting in irrigation schemes.

scholarly journals Older children are at increased risk of Plasmodium vivax in south-central Ethiopia: a cohort study

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Taye Gari ◽  
Tarekegn Solomon ◽  
Bernt Lindtjørn
Keyword(s):  
Risk Factors ◽  
Plasmodium Vivax ◽  
Incidence Rate ◽  
Breeding Site ◽  
Age Group ◽  
Breeding Sites ◽  
Central Ethiopia ◽  
South Central ◽  
Increased Risk ◽  
Mosquito Breeding

Abstract Background Better understanding of the distribution of Plasmodium vivax and its risk factors could be used to prevent and control malaria infection. Therefore, the aim of this study was to characterize the distribution and risk factors of P. vivax, and to compare them with Plasmodium falciparum occurrence in south-central Ethiopia. Methods A cohort of 34,548 individuals were followed for 121 weeks between 2014 and 2016 as part of larger cluster randomized controlled trial to evaluate the effect of long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) on malaria prevention in Ethiopia. Weekly home visit (active search) and patient self- report to health post (passive search) between the weekly home visits were used to register malaria cases. A blood sample was collected by finger prick and malaria was diagnosed using rapid diagnostic test (RDT). Generalized estimating equation (GEE) Poisson model that accounts for repeated measure of malaria episodes was applied to assess the risk factors of P. vivax episode. Results The overall incidence rate of P. vivax was 7.4 episodes per 1000 person-years of observation. The study showed households closer to the lake Zeway and Bulbula river (potential mosquito breeding sites) were more at risk of P. vivax infection (incidence rate ratio (IRR): 1.33; 95% CI = 1.23–1.45). Furthermore, the age group under 5 years (IRR: 1.40, 95% CI = 1.10–1.79), the age group 5–14 years (IRR: 1.27, 95% CI = 1.03–1.57), households with less educated household head (IRR: 1.63, 95% CI = 1.10–2.44) and house roof made of thatch/leaf (IRR: 1.35, 95% CI = 1.11–1.65) were at higher risk for P. vivax. Similar explanatory variables such as distance from the breeding sites, age group (under 5 years but not 5–14 years old), educational status and type of housing were also found to be the predictors of P. falciparum incidence. Conclusion Households living closer to a mosquito breeding site, age group under 15 years, less educated household heads and thatch/leaf roof housing were the risk factor for P. vivax. The result of this study can be used for tailored interventions for malaria control and prevention by prioritizing those living close to potential mosquito breeding site, enhancing bed net use of children less than 15 years of age, and improving housing.

scholarly journals The community of Diptera (Insecta) colonizing axils of Alocasia macrorrhizos (L.) G.Don (Araceae), with records of Aedes aegypti (L.) and Aedes albopictus (Skuse) in urban areas of Manaus, Amazonas

2017 ◽  
Vol 17 (3) ◽  
Author(s):  
Ruth Leila Ferreira-Keppler ◽  
Ulisses Gaspar Neiss ◽  
Sharlene Roberta da Silva Torreias ◽  
Claudimir Menezes Campos
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Aquatic Insects ◽  
Urban Areas ◽  
Breeding Site ◽  
Adult Plant ◽  
Forest Fragments ◽  
Positive Relation ◽  
Important Species ◽  
Breeding Sites

Abstract Alocasia macrorrhizos (L.) G.Don grows and proliferates in shaded areas in several forest fragments in urban zones. The adult plant has axils that accumulate rain water (phytotelmata) and serve as breeding sites for several families of aquatic insects. The objective of this study was to determine the composition of the entomofauna associated with water accumulations in axils of A. macrorrhizos in urban areas of Manaus, Brazil. Individuals of A. macrorrhizos were sampled in five forest fragments, between April/2005 and March/2007 in urban areas of Manaus, totaling 184 sampling units. A manual suction hose was used for the removal of entomofauna. A total of 1,941 immatures of Diptera were collected representing the following families: Culicidae (96.5%), Chironomidae (2%), Psychodidae (0.7%), Ephydridae (0.7%) and Corethrellidae (0.1%). Five Culicidae species were identified: Wyeomyia melanocephala Dyar & Knab, 1906 (91.7%), Wy. ypsipola Dyar, 1922 (4.3%), Aedes albopictus (Skuse, 1894) (2.3%), Ae. aegypti (Linnaeus, 1762) (1.5%) and Johnbelkinia longipes (Fabricius, 1805) (0.1%). The members of Culicidae were the most abundant, confirming the importance of this group in communities associated with phytotelmata, with a positive relation to the volume of water (p < 0.05). The occurrence of Ae. aegypti and Ae. albopictus in axils of A. macrorrhizos demonstrates the potential of this Araceae as a breeding site of these medically important species. However, the real contribution of this Araceae in the maintenance and dispersion of populations of these two species of mosquitoes throughout the year should be further studied.

scholarly journals Continued Susceptibility of the wMel Wolbachia Infection in Aedes aegypti to Heat Stress Following Field Deployment and Selection

Insects ◽  
2018 ◽  
Vol 9 (3) ◽  
pp. 78 ◽  
Author(s):  
Perran Ross ◽  
Ary Hoffmann
Keyword(s):  
Heat Stress ◽  
Aedes Aegypti ◽  
High Temperatures ◽  
Wolbachia Infection ◽  
Field Exposure ◽  
Breeding Sites ◽  
Laboratory Selection ◽  
Mosquito Breeding ◽  
Field Deployment ◽  
Cyclical Heat

Aedes aegypti mosquitoes infected with the wMel strain of Wolbachia are being deployed to control the spread of arboviruses around the world through blockage of viral transmission. Blockage by Wolbachia in some scenarios may be affected by the susceptibility of wMel to cyclical heat stress during mosquito larval development. We therefore evaluated the potential to generate a heat-resistant strain of wMel in Ae. aegypti through artificial laboratory selection and through exposure to field temperatures across multiple generations. To generate an artificially selected strain, wMel-infected females reared under cyclical heat stress were crossed to wMel-infected males reared at 26 °C. The low proportion of larvae that hatched founded the next generation, and this process was repeated for eight generations. The wMel heat-selected strain (wMel-HS) was similar to wMel (unselected) in its ability to induce cytoplasmic incompatibility and restore compatibility when larvae were reared under cyclical heat stress, but wMel-HS adults exhibited reduced Wolbachia densities at 26 °C. To investigate the effects of field exposure, we compared the response of wMel-infected Ae. aegypti collected from Cairns, Australia where the infection has been established for seven years, to a wMel-infected population maintained in the laboratory for approximately 60 generations. Field and laboratory strains of wMel did not differ in their response to cyclical heat stress or in their phenotypic effects at 26 °C. The capacity for the wMel infection in Ae. aegypti to adapt to high temperatures therefore appears limited, and alternative strains may need to be considered for deployment in environments where high temperatures are regularly experienced in mosquito breeding sites.

scholarly journals Indeks Entomologi dan Sebaran Vektor Demam Berdarah Dengue di Provinsi Maluku Utara Tahun 2015

2018 ◽  
Vol 28 (4) ◽  
pp. 279-288
Author(s):  
Dian Perwitasari ◽  
Roy Nusa RES ◽  
Jusniar Ariati
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Urban Areas ◽  
Hemorrhagic Fever ◽  
Risk Category ◽  
Cross Sectional ◽  
Breeding Sites ◽  
The North ◽  
Medium Risk ◽  
Mosquito Breeding

One effort in controlling the Aedes aegypti mosquito is to find out the breeding place. Until now it is known that the breeding sites of Aedes aegypti are found in urban areas, therefore the purpose of this study is to determine the  potential for breeding of Aedes aegypti mosquito and entomology index in urban areas. The study was conducted in 2015 using a cross-sectional method. The results showed that the container with positively Aedes aegypti larvae was found in all three districts, Ternate at 29.6%, Tidore Kepulauan at 28.5% and East Halmahera at 29.0%. The entomology index of Container Index (CI) was (29.5%), House Index (HI) was 35.3% and Breteau Index (BI) was 69.2%, while larva free number was 64.7%. From the results of this study, it was concluded that Aedes aegypti larvae mostly found in bathtub inside the house. Tubs and plastic buckets were the type of container that were found with most positively larvae. The average ldengue vector arvae density based on CI, HI and BI (Aedes aegypti and Aedes albopictus) in the three districts / cities in North Maluku Province scale was 5-8 and included in the medium risk category. This showed that the entomology index in the North Maluku region was still low. Monitoring of the spread of mosquito breeding sites in all parts of Indonesia is still needed to reduce cases of Dengue Hemorrhagic Fever (DHF). Abstrak Salah satu upaya dalam pengendalian nyamuk Aedes aegypti adalah dengan mengetahui tempat perkembangbiakannya. Hingga saat ini diketahui bahwa tempat perkembangbiakan Aedes aegypti banyak ditemukan di daerah perkotaan, oleh sebab itu tujuan dari penelitian ini adalah untuk mengetahui potensi perkembangbiakan nyamuk Aedes aegypti dan indeks entomologi di daerah perkotaan. Penelitian dilakukan pada tahun 2015 dengan menggunakan metode potong lintang. Hasil menunjukkan bahwa kontainer dengan positif jentik Aedes aegypti ditemukan di ketiga kabupaten yaitu Ternate sebesar 29,6%, Tidore Kepulauan sebesar 28,5%, dan Halmahera Timur sebesar 29,0%. Indeks entomologi Container Index (CI) sebesar (29,5%), House Index (HI) sebesar 35,3% dan Breteau Index (BI) sebesar 69,2%, sedangkan Angka Bebas Jentik sebesar 64,7%. Dari hasil penelitian ini didapatkan kesimpulan bahwa bak mandi yang berada di dalam rumah paling banyak ditemukan jentik Aedes aegypti. Jenis kontainer yang paling banyak ditemukan positif jentik yaitu pada bak mandi dan ember plastik. Rata-rata kepadatan jentik vektor dengue (Aedes aegypti dan Aedes albopictus) berbasis CI, HI, dan BI di ketiga kabupaten/kota di Provinsi Maluku Utara skala 5-8 dan termasuk dalam kategori risiko sedang. Hal ini menunjukkan bahwa indeks entomologi di wilayah Maluku Utara masih rendah, sehingga masih diperlukan pemantauan sebaran tempat perkembangbiakan nyamuk di seluruh wilayah Indonesia untuk mengurangi kasus Demam Berdarah Dengue (DBD).

Sign in / Sign up

Export Citation Format

Share Document