scholarly journals Functional Analysis of the Gibberellin 2-oxidase Gene Family in Peach

2021 ◽  
Vol 12 ◽  
Author(s):  
Jun Cheng ◽  
Jingjing Ma ◽  
Xianbo Zheng ◽  
Honglin Lv ◽  
Mengmeng Zhang ◽  
...  
Keyword(s):  
Gene Family ◽  
Prunus Persica ◽  
Response Times ◽  
Profile Analysis ◽  
Fine Tuning ◽  
Oxidase Gene ◽  
Peach Genome ◽  
Vigorous Growth ◽  
Different Response ◽  
Peach Trees

Peach (Prunus persica L. Batsch) trees grow vigorously and are subject to intense pruning during orchard cultivation. Reducing the levels of endogenous gibberellins (GAs) represents an effective method for controlling branch growth. Gibberellin 2-oxidases (GA2oxs) deactivate bioactive GAs, but little is known about the GA2ox gene family in peach. In this study, we identified seven PpGA2ox genes in the peach genome, which were clustered into three subgroups: C19-GA2ox-I, C19-GA2ox-II, and C20-GA2ox-I. Overexpressing representative genes from the three subgroups, PpGA2ox-1, PpGA2ox-5, and PpGA2ox-2, in tobacco resulted in dwarf plants with shorter stems and smaller leaves than the wild type. An analysis of the GA metabolic profiles of the transgenic plants showed that PpGA2ox-5 (a member of subgroup C19-GA2ox-II) is simultaneously active against both C19-GAs and C20-GAs,which implied that C19-GA2ox-II enzymes represent intermediates of C19-GA2oxs and C20-GA2oxs. Exogenous GA3 treatment of shoot tips activated the expression of all seven PpGA2ox genes, with different response times: the C19-GA2ox genes were transcriptionally activated more rapidly than the C20-GA2ox genes. GA metabolic profile analysis suggested that C20-GA2ox depletes GA levels more broadly than C19-GA2ox. These results suggest that the PpGA2ox gene family is responsible for fine-tuning endogenous GA levels in peach. Our findings provide a theoretical basis for appropriately controlling the vigorous growth of peach trees.

Genome-Wide Identification and Analysis of the Type-B Authentic Response Regulator Gene Family in Peach (Prunus persica)

2017 ◽  
Vol 151 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Jingjue Zeng ◽  
Xudong Zhu ◽  
Muhammad S. Haider ◽  
Xicheng Wang ◽  
Cheng Zhang ◽  
...  
Keyword(s):  
Gene Family ◽  
Stress Responses ◽  
Prunus Persica ◽  
Response Regulator ◽  
Expression Profiles ◽  
Protein Domain ◽  
Future Research ◽  
Type B ◽  
Cytokinin Signaling ◽  
Peach Genome

The type-B authentic response regulator (ARR-B) family members serve as DNA-binding transcriptional regulators, whose activities are probably regulated by phosphorylation/dephosphorylation, resulting in the rapid induction of type-A ARR genes. Type-B ARRs are believed to be involved in many biological processes, including cytokinin signaling, plant growth, and stress responses through a chaperone or by isomerization of proline residues during protein folding. The public availability of complete peach genome sequences allows the identification of 23 ARR-B genes by HMMER and blast analysis. Scaffold locations of these genes in the peach genome were determined, and the protein domain and motif organization of peach type-B ARRs were analyzed. The phylogenetic relationships between peach type-B ARRs were also assessed. The expression profiles of peach ARR-B genes revealed that most of the type-B ARRs showed high expression levels in tissues undergoing rapid cell division and may engage more cytokinins, like half-opened flowers, fruits at expansion stages, and young leaves. These findings not only contribute to a better understanding of the complex regulation of the peach ARR-B gene family, but also provide valuable information for future research in peach functional genomics.

scholarly journals Genome wide analysis of kinesin gene family in Citrullus lanatus reveals an essential role in early fruit development

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shujuan Tian ◽  
Jiao Jiang ◽  
Guo-qi Xu ◽  
Tan Wang ◽  
Qiyan Liu ◽  
...  
Keyword(s):  
Gene Family ◽  
Plant Hormones ◽  
Fruit Development ◽  
Developmental Stages ◽  
Profile Analysis ◽  
Citrullus Lanatus ◽  
Binding Motif ◽  
Distribution Analysis ◽  
Motif Analysis ◽  
Expression Profile Analysis

Abstract Background Kinesin (KIN) as a motor protein is a versatile nano-machine and involved in diverse essential processes in plant growth and development. However, the kinesin gene family has not been identified in watermelon, a valued and nutritious fruit, and yet their functions have not been characterized. Especially, their involvement in early fruit development, which directly determines the size, shape, yield and quality of the watermelon fruit, remains unclear. Results In this study, we performed a whole-genome investigation and comprehensive analysis of kinesin genes in C. lanatus. In total, 48 kinesins were identified and categorized into 10 kinesin subfamilies groups based on phylogenetic analysis. Their uneven distribution on 11 chromosomes was revealed by distribution analysis. Conserved motif analysis showed that the ATP-binding motif of kinesins was conserved within all subfamilies, but not the microtubule-binding motif. 10 segmental duplication pairs genes were detected by the syntenic and phylogenetic approaches, which showed the expansion of the kinesin gene family in C. lanatus genome during evolution. Moreover, 5 ClKINs genes are specifically and abundantly expressed in early fruit developmental stages according to comprehensive expression profile analysis, implying their critical regulatory roles during early fruit development. Our data also demonstrated that the majority of kinesin genes were responsive to plant hormones, revealing their potential involvement in the signaling pathways of plant hormones. Conclusions Kinesin gene family in watermelon was comprehensively analyzed in this study, which establishes a foundation for further functional investigation of C. lanatus kinesin genes and provides novel insights into their biological functions. In addition, these results also provide useful information for understanding the relationship between plant hormone and kinesin genes in C. lanatus.

scholarly journals Fine-tuning the performance of ddRAD-seq in the peach genome

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Maximiliano Martín Aballay ◽  
Natalia Cristina Aguirre ◽  
Carla Valeria Filippi ◽  
Gabriel Hugo Valentini ◽  
Gerardo Sánchez
Keyword(s):  
Germplasm Collection ◽  
In Silico Analysis ◽  
Genotyping By Sequencing ◽  
Fine Tuning ◽  
The Novel ◽  
Peach Genome ◽  
Genome Complexity ◽  
Next Generation Sequencing Ngs ◽  
Sample Set ◽  
Silico Analysis

AbstractThe advance of Next Generation Sequencing (NGS) technologies allows high-throughput genotyping at a reasonable cost, although, in the case of peach, this technology has been scarcely developed. To date, only a standard Genotyping by Sequencing approach (GBS), based on a single restriction with ApeKI to reduce genome complexity, has been applied in peach. In this work, we assessed the performance of the double-digest RADseq approach (ddRADseq), by testing 6 double restrictions with the restriction profile generated with ApeKI. The enzyme pair PstI/MboI retained the highest number of loci in concordance with the in silico analysis. Under this condition, the analysis of a diverse germplasm collection (191 peach genotypes) yielded 200,759,000 paired-end (2 × 250 bp) reads that allowed the identification of 113,411 SNP, 13,661 InDel and 2133 SSR. We take advantage of a wide sample set to describe technical scope of the platform. The novel platform presented here represents a useful tool for genomic-based breeding for peach.

Herbicide Effects on Weed Control and Shoot Growth of Young Apple (Malus sylvestris) and Peach (Prunus persica) Trees

1994 ◽  
Vol 8 (4) ◽  
pp. 840-848 ◽  
Author(s):  
Chester L. Foy ◽  
Susan B. Harrison ◽  
Harold L. Witt
Keyword(s):  
Shoot Growth ◽  
Field Experiments ◽  
Prunus Persica ◽  
Weed Species ◽  
Apple Trees ◽  
Herbicide Treatments ◽  
One Year ◽  
Broadleaf Species ◽  
Old Trees ◽  
Peach Trees

Field experiments were conducted at two locations in Virginia to evaluate the following herbicides: alachlor, diphenamid, diuron, metolachlor, napropamide, norflurazon, oryzalin, oxyfluorfen, paraquat, pendimethalin, and simazine. One experiment involved newly-transplanted apple trees; the others, three in apple and one in peach trees, involved one-year-old trees. Treatments were applied in the spring (mid-April to early-May). Control of annual weed species was excellent with several treatments. A broader spectrum of weeds was controlled in several instances when the preemergence herbicides were used in combinations. Perennial species, particularly broadleaf species and johnsongrass, were released when annual species were suppressed by the herbicides. A rye cover crop in nontreated plots suppressed the growth of weeds. New shoot growth of newly-transplanted apple trees was increased with 3 of 20 herbicide treatments and scion circumference was increased with 11 of 20 herbicide treatments compared to the nontreated control. Growth of one-year-old apple trees was not affected. Scion circumference of one-year-old peach trees was increased with 25 of 33 herbicide treatments.

Early performance of micropropagated trees of several Malus and Prunus cultivars on their own roots

1993 ◽  
Vol 73 (3) ◽  
pp. 847-855 ◽  
Author(s):  
H. A. Quamme ◽  
R. T. Brownlee
Keyword(s):  
Sweet Cherry ◽  
Prunus Persica ◽  
Fruit Size ◽  
Sour Cherry ◽  
Titratable Acidity ◽  
Soluble Solids ◽  
Flesh Firmness ◽  
Golden Delicious ◽  
Early Performance ◽  
Peach Trees

Early performance (6–8 yr) of Macspur McIntosh, Golden Delicious, and Spartan apple (Malus domestica Borkh.); Fairhaven peach [Prunus persica (L.) Batsch.]; Montmorency sour cherry (P. cerasus L.); and Lambert sweet cherry (P. avium L.) trees, tissue cultured (TC) on their own roots, was compared with that of the same cultivars budded on commercially used rootstocks. TC trees of all apple cultivars were similar in size to trees budded on Antonovka seedling or M.4 and exceeded the size of trees budded on M.26. They were delayed in flowering and in cropping compared with trees budded on M.26 and M.4. No difference in titratable acidity, soluble solids, flesh firmness, weight, flavor, and color between fruit from TC trees and from trees on M.4 and Antonovka seedlings was detected in 1 yr of measurement. However, fruit from TC Golden Delicious was more russeted and fruit from TC Spartan had more soluble solids. The difference in fruit appearance between TC and budded trees may result from a root-stock effect or a difference in budwood source, because Spartan fruit from trees on M.4 was more russeted than Spartan fruit from TC trees, but was not different from Spartan fruit from trees on Antonovka seedling. Trees of Macspur McIntosh on TC M.26 and on stool-layered M.26 were similar in size and yield efficiency. TC Fairhaven was larger in size than Fairhaven on Siberian C seedling, but was less yield efficient. No difference in fruit size, flesh firmness, or color was detected between fruit harvested from peach trees on the different roots. Montmorency and Lambert TC and on F12/1 were similar in tree size, respectively, but Montmorency and Lambert TC were more yield efficient than on F12/1. Fruit of TC Lambert was lighter in color and had higher titratable acidity than that of Lambert on F12/1, perhaps a result of earlier fruit maturity. Key words: Apple, peach, sweet cherry, sour cherry, self-rooted, rootstocks

scholarly journals Nematode Populations and Peach Tree Survival, Growth, and Nutrition at an Old Orchard Site

1992 ◽  
Vol 117 (1) ◽  
pp. 6-13 ◽  
Author(s):  
Dean R. Evert ◽  
Paul F. Bertrand ◽  
`Benjamin G. Mullinix
Keyword(s):  
Tree Growth ◽  
Prunus Persica ◽  
Paspalum Notatum ◽  
Parasitic Nematodes ◽  
Severe Stunting ◽  
Growth And Nutrition ◽  
Tree Survival ◽  
Peach Trees ◽  
Positive Effect ◽  
Survival Tree

Bahiagrass (Paspalum notatum Flugge cv. Paraguayan-22) growing under newly planted peach [Prunus persica (L.) Batsch.] trees severely stunted the trees. Neither supplemental fertilizer nor irrigating with two 3.8-liters·hour-1 emitters per tree eliminated tree stunting emitters were controlled by an automatic tensiometer set to maintain 3 kpa at a depth of 0.5 m under a tree in bahiagrass. Preplant fumigation with ethylene dibromide at 100 liters·ha-1 increased tree growth, but not tree survival. Fenamiphos, a nematicide, applied under the trees each spring and fall at a rate of 11 kg-ha -1 had no positive effect on tree survival, tree growth, or nematode populations. Bahiagrass tended to suppress populations of Meloidogyne spp. under the trees., Meloidogyne spp. were the only nematodes present that had mean populations > 65 per 150 cm3 of soil. Leaf concentrations of several elements differed between trees growing in bahiagrass sod and in. bare ground treated with herbicides. Leaf Ca was low for all treatments in spite of a soil pH near 6.5 and adequate soil Ca. The severe stunting of trees grown in bahiagrass, irrespective of the other treatments, demonstrated that bahiagrass should not be grown under newly planted trees. The low populations of parasitic nematodes in bahiagrass showed that bahiagrass has potential as a preplant biological control of nematodes harmful to peach trees. Chemical name used: ethyl 3-methy1-4-(methylthio) phenyl (1-methylethyl) phosphoramidate (fenamiphos).

Formation of the primary protective layer and phellogen after leaf abscission in peach

1985 ◽  
Vol 63 (9) ◽  
pp. 1547-1550 ◽  
Author(s):  
A. R. Biggs ◽  
J. Northover
Keyword(s):  
Prunus Persica ◽  
Separation Zone ◽  
Protective Layer ◽  
Leaf Abscission ◽  
Subsequent Generation ◽  
Layer Formation ◽  
Influence Of Temperature ◽  
Influence Of Temperature On ◽  
Peach Trees

Potted peach trees (Prunus persica (L.) Batsch cv. Loring) were mechanically defoliated and the influence of temperature on formation of the primary protective layer and phellogen generation in the leaf abscission region was examined histologically. Plants maintained at 7.5, 12.5, and 17.5 °C showed first indications of primary protective layer formation at 18, 9, and 6 days, respectively. Subsequent generation of phellogen and the appearance of the first phellem cell were observed at 30, 18, and 12 days, respectively. The primary protective layer formed approximately 700 μm proximal to the separation zone and was composed of cells with lignified walls and thin suberin linings.

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