scholarly journals Appropriate NH4+/NO3– Ratio Triggers Plant Growth and Nutrient Uptake of Flowering Chinese Cabbage by Optimizing the pH Value of Nutrient Solution

2021 ◽  
Vol 12 ◽  
Author(s):  
Yunna Zhu ◽  
Baifu Qi ◽  
Yanwei Hao ◽  
Houcheng Liu ◽  
Guangwen Sun ◽  
...  

Compared with sole nitrogen (N), the nutrition mixture of ammonium (NH4+) and nitrate (NO3–) is known to better improve crop yield and quality. However, the mechanism underlying this improvement remains unclear. In the present study, we analyzed the changes in nutrient solution composition, content of different N forms in plant tissues and exudates, and expression of plasma membrane (PM) H+-ATPase genes (HAs) under different NH4+/NO3– ratios (0/100, 10/90, 25/75, 50/50 as control, T1, T2, and T3) in flowering Chinese cabbage. We observed that compared with the control, T1 and T2 increased the economical yield of flowering Chinese cabbage by 1.26- and 1.54-fold, respectively, whereas T3 significantly reduced plant yield. Compared with the control, T1–T3 significantly reduced the NO3– content and increased the NH4+, amino acid, and soluble protein contents of flowering Chinese cabbage to varying extents. T2 significantly increased the N use efficiency (NUE), whereas T3 significantly decreased it to only being 70.25% of that of the control. Owing to the difference in N absorption and utilization among seedlings, the pH value of the nutrient solution differed under different NH4+/NO3– ratios. At harvest, the pH value of T2 was 5.8; in the control and T1, it was approximately 8.0, and in T3 it was only 3.6. We speculated that appropriate NH4+/NO3– ratios may improve N absorption and assimilation and thus promote the growth of flowering Chinese cabbage, owing to the suitable pH value. On the contrary, addition of excessive NH4+ may induce rhizosphere acidification and ammonia toxicity, causing plant growth inhibition. We further analyzed the transcription of PM H+-ATPase genes (HAs). HA1 and HA7 transcription in roots was significantly down-regulated by the addition of the mixture of NH4+ and NO3–, whereas the transcription of HA2, HA9 in roots and HA7, HA8, and HA10 in leaves was sharply up-regulated by the addition of the mixture; the transcription of HA3 was mainly enhanced by the highest ratio of NH4+/NO3–. Our results provide valuable information about the effects of treatments with different NH4+/NO3– ratios on plant growth and N uptake and utilization.

2012 ◽  
Vol 461 ◽  
pp. 3-6
Author(s):  
Shi Wei Song ◽  
Qiu Yan Yan ◽  
Fei Dong ◽  
Hou Cheng Liu ◽  
Guang Wen Sun ◽  
...  

The effect of color shading-nets (red net, blue net and silver net) and ordinary black net on plant growth and nutrient uptake of flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) were studied, with no net shading as the control. The results indicated that, compared with the control, red net covering increased plant height, leaf area and stem diameter of flowering Chinese cabbage. Red and blue nets covering enhanced the above ground fresh weight, while the silver and black nets covering decreased the above ground fresh weight. Shading net covering significantly improved the nitrogen (N) and potassium (K) content of flowering Chinese cabbage, while it had no significant effect on the phosphorus (P) content. Red and blue nets covering enhanced mineral nutrients uptake, while it was decreased under silver and black nets covering. Red and blue nets covering promoted plant growth and nutrients uptake of flowering Chinese cabbage and could be widely applied in leaf vegetable production.


Author(s):  
Abdelmajid Krouma

Calcareous soils are known problematic lands for agricultural systems because of the low availability of nutrients, particularly iron (Fe). The so-called strategy I plant (e. g. Pea, Pisum sativum L.) which groups dicotyledons and monocots other than grasses, developed root membrane activities that contribute to the improvement of Fe availability. Among the functions considered to be a critical phase in iron absorption is rhizosphere acidification by H-ATPase and Fe(III) reduced by Fe(III) chelate reducctase (FeCR). In order to experimentally investigate the importance of root FeCR in Fe nutrition, its relationship with rhizosphere acidification and the genotypic differences in response to iron deficiency in pea (Pisum sativum L.), a glasshouse experiment was conducted hydroponically on four genotypes Merveille de Kelvedon (MK); Lincoln (Lin); Douce de Provence (DP) and Alexandra (Alex). Plants of each genotype were distributed into two plots, the first one received full nutrient solution (+ Fe), the second one received nutrient solution devoid of iron (- Fe). Plant growth, Fe distribution, SPAD index and root acidification and ferric chelate reductase activities were evaluated. Fe deficiency decreased plant growth and SPAD index along with the significant increase of H-ATPase and FeCR activities. Some genotypic differences were observed as follows; Alex showed high tolerance to Fe deprivation as compared to other genotypes. Important H-ATPase and FeCR activities, high Fe use efficiency and adequate membrane efficiency are the main reasons for this tolerance. These physiological parameters could be used as tools of tolerance for further breeding programs


Author(s):  
Golgen Bahar Oztekin ◽  
Tuğba Uludağ ◽  
Yüksel Tüzel ◽  
Mahmut Tepecik

This study was conducted in styrofoam trays placed on an aerated nutrient solution in vats in order to determine the effect of different nutrient solutions on rocket plant growth, yield and quality. Seeds of Bengi F1 rocket cultivar were sown into peat as inserting each seed to each hole (17 ccs) of trays with 210 cells (957 plant m-2). Following germination in the germination chamber, seedling trays were moved to a climate controlled greenhouse for adaptation. After emergence, the seedlings were transferred to water culture. The nutrient solution was applied as “full dose” (mg/L: N 150, P 50, K 150, Ca 150, Mg 50, Fe 5.0, Mn 0.50, Zn 0.05, B 0.50, Cu 0.03, Mo 0.02), “half dose” (macro elements reduced by 50%) and “without nutrients” (water). Cultivation was performed in 2 consecutive periods and in each period 3 harvests were done and quality analysis was done on the leaves from the first harvest. Results were given as the mean of two periods. Results showed that plant growth, yield and quality parameters vary depending on the concentration of the nutrient solution. It was observed that as the concentration of nutrient solution decreased, plant growth, biomass, yield values decreased, leaf color and total chlorophyll concentration did not change and while nitrate content decreased, vitamin C content increased. Leaf N, P, K and Fe element contents decreased with half dose, whereas Ca and Mg content did not change. However, there was no statistical difference between full dose and half dose treatments in many measured parameters. When all the data obtained from the study are evaluated together; it is suggested that rocket can be grown in a floating water culture and the dose of macro elements as reduced 50% can be preferred because it reduces the nitrate content, increases the vitamin C content and allows the use of less fertilizers without causing any significant reduction in yield compared with full dose.


2011 ◽  
Vol 142 ◽  
pp. 188-192 ◽  
Author(s):  
Shi Wei Song ◽  
Ling Yan Yi ◽  
Hou Cheng Liu ◽  
Guang Wen Sun ◽  
Ri Yuan Chen

Effect of ammonium and nitrate ratios (0:100, 25:75, 50:50 and 75:25) on nutritional quality of flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) with 3 cultivars were studied in hydroponics. The results indicated that, with the increasing of ammonium proportion in nutrient solution, the soluble sugar content in product organ of flowering Chinese cabbage increased gradually, but Vc content decreased gradually. Enhancement of ammonium by 25% or 50% in nutrient solution increased the soluble protein content in leaf significantly, while it increased gradually in stalk with the increasing of ammonium proportion. Free amino acids in product organ of flowering Chinese cabbage were improved significantly by enhancement of ammonium in nutrient solution. Integrated nutritional quality of flowering Chinese cabbage in 25% ammonium enhancement treatment was better than others under hydroponics condition.


2021 ◽  
Author(s):  
Xian Yang

Abstract Iron (Fe) plays an important role in the growth and development of the human body and plants. The effects of different Fe concentrations, 1-aminocyclopropane-1-carboxylic acid (ACC), and cobalt chloride (Co2+) treatments on plant growth, quality and the adaptive response to Fe deficiency stress were investigated in flowering Chinese cabbage. The results revealed that Fe deficiency stress inhibited plant growth. The content of vitamin C (Vc), soluble protein, and soluble sugar in leaves and stalks were significantly reduced under Fe deficiency stress, while the content of cellulose and nitrate was increased. Meanwhile, Fe deficiency stress obviously reduced the net photosynthetic rate and nitrate reductase (NR) activity of leaves. The balance system of active oxygen metabolism was destroyed due to Fe deficiency, resulting in the decrease of catalase (CAT) activity, superoxide dismutase (SOD) activity of roots and leaves, and peroxidase (POD) activity of leaves, while POD activity in roots and malonaldehyde (MDA) content were significantly increased. The treatments of Fe deficiency and ACC significantly reduced pH value of the root medium, promoted release of ethylene, and increased Fe3+ reductase activity, while Co2+ treatment showed the results opposite to those of Fe deficiency and ACC treatments. Thus, Fe deficiency stress could induce nitrogen metabolism, photosynthesis, reactive oxygen metabolism, pH of root medium, and Fe3+ reductase activity that was related to physiological adaptive response and tolerance mechanisms. We also found that ethylene could involve in regulating the adaptive response to Fe deficiency stress and improve the availability of Fe in flowering Chinese cabbage.Main ConclusionFe deficiency stress could induce nitrogen metabolism, photosynthesis, reactive oxygen metabolism, pH of root medium, and Fe3+ reductase activity that was related to physiological adaptive response and tolerance mechanisms.


2011 ◽  
Vol 29 (4) ◽  
pp. 516-519 ◽  
Author(s):  
Jerônimo L Andriolo ◽  
Lígia Erpen ◽  
Francieli L Cardoso ◽  
Carine Cocco ◽  
Gustavo S Casagrande ◽  
...  

In soilless grown strawberry crops, the nitrogen concentration of the nutrient solution affects plant growth and fruit yield and quality. The present research was conducted to determine the effect of nitrogen concentration in the nutrient solution on plant growth and development and fruit yield and quality of this crop. Treatments consisted of five nutrient solutions at nitrogen concentrations of 6.5 (T1), 8.0 (T2), 9.5 (T3), 11.0 (T4) and 12.5 (T5) mmol L-1, in an entirely randomised experimental design with four replications. Ripe fruit yield was determined during the harvest period from June 6th to November 27th, 2009. Number of leaves, shoot and root dry mass and crown diameter were determined at the later date. Number of leaves, shoot and root dry mass and crown diameter decreased by effect of increasing N concentrations in the nutrient solution. Fruit yield and fruit size fitted a polynomial model, with maximum values at 8.9 mmol N L-1. The N concentration used for the strawberry crop in soilless growing systems can be reduced to 8.9 mmol L-1 without any reduction in fruit yield.


Horticulturae ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 8
Author(s):  
Yudan Wang ◽  
Xiaoyun Zhang ◽  
Houcheng Liu ◽  
Guangwen Sun ◽  
Shiwei Song ◽  
...  

The aim of this study was to determine the effects of various NH4+/NO3− ratios in a nutrient solution on the growth and nitrogen uptake of Chinese kale under hydroponic conditions. The four NH4+/NO3− ratios in the nutrient solution were CK (0/100), T1 (10/90), T2 (25/75), and T3 (50/50). An appropriate NH4+/NO3− ratio (10/90, 25/75) promoted the growth of Chinese kale. T2 produced the highest fresh and dry weight among treatments, and all indices of seedling root growth were the highest under T2. A high NH4+/NO3− ratio (50/50) promoted the growth of Chinese kale seedlings at the early stage but inhibited growth at the late growth stage. At harvest, the nutrient solution showed acidity. The pH value was the lowest in T3, whereas NH4+ and NH4+/NO3− ratios were the highest, which caused ammonium toxicity. Total N accumulation and N use efficiency were the highest in T2, and total N accumulation was the lowest in T3. Principal component analysis showed that T2 considerably promoted growth and N absorption of Chinese kale, whereas T3 had a remarkable effect on the pH value. These findings suggest that an appropriate increase in NH4+ promotes the growth and nutrient uptake of Chinese kale by maintaining the pH value and NH4+/NO3− ratios of the nutrient solution, whereas excessive addition of NH4+ may induce rhizosphere acidification and ammonia toxicity, inhibiting plant growth.


2007 ◽  
Vol 17 (2) ◽  
pp. 227-233 ◽  
Author(s):  
Alejandro R. Puerta ◽  
Suguru Sato ◽  
Yutaka Shinohara ◽  
Toru Maruo

In nutrient film technique (NFT) vegetable production, the use of low-concentration nutrient solutions might lead to a nutrient concentration gradient along the bed, which can translate into nonuniform plant growth. The authors modified a conventional NFT system (cNFT) and propose a modified NFT (mNFT) that enables the production of lettuce (Lactuca sativa) plants of uniform fresh weight along elongated cultural beds. Two experiments were carried out to compare the systems when long cultural beds are used (18 m) in terms of uniformity of plant and nutrient solution characteristics. The results indicated that fresh weight of plants in cNFT decreased as the distance from the nutrient inlet increased, whereas no such trend was observed in mNFT. Leaf nitrate concentration in mNFT was uniform, whereas it was higher near the outlet of cNFT. Ascorbic acid concentration was also uniform in mNFT, but it was found to be lower near the outlet of cNFT. During Expt. 2, the oxygen content along the bed decreased from inlet to outlet in cNFT; however, in mNFT, it remained relatively constant at all sampling positions. Regarding the concentration of the nutrient solution along beds, no significant differences were found between inlet and outlet in both systems. It is suggested that the lower concentration of oxygen found at the outlet of cNFT might have reduced nutrient uptake, thus attenuating the difference in concentration between inlet and outlet. The temperatures of the nutrient solution along mNFT during Expt. 2 tended to be slightly lower than those of cNFT. However, temperatures were still too high and plant growth was negatively affected. The results of this study demonstrate that plants of uniform size and quality can be achieved in long cultural beds (up to 18 m long) supplied with a low-concentration fertilizer solution by using the proposed mNFT.


Author(s):  
R.H. Li ◽  
Y.S. Xia ◽  
Y. Li ◽  
L.G. Xu ◽  
H. Zhang ◽  
...  

Author(s):  
V. Dumych ◽  

The purpose of research: to improve the technology of growing flax in the Western region of Ukraine on the basis of the introduction of systems for minimizing tillage, which will increase the yield of trusts and seeds. Research methods: field, laboratory, visual and comparative calculation method. Research results: Field experiments included the study of three tillage systems (traditional, canning and mulching) and determining their impact on growth and development and yields of trusts and flax seeds. The traditional tillage system included the following operations: plowing with a reversible plow to a depth of 27 cm, cultivation with simultaneous harrowing and pre-sowing tillage. The conservation system is based on deep shelfless loosening of the soil and provided for chiseling to a depth of 40 cm, disking to a depth of 15 cm, cultivation with simultaneous harrowing, pre-sowing tillage. During the implementation of the mulching system, disking to a depth of 15 cm, cultivation with simultaneous harrowing and pre-sowing tillage with a combined unit was carried out. Tillage implements and machines were used to perform tillage operations: disc harrow BDVP-3,6, reversible plow PON-5/4, chisel PCh-3, cultivator KPSP-4, pre-sowing tillage unit LK-4. The SZ-3,6 ASTPA grain seeder was used for sowing long flax of the Kamenyar variety. Simultaneously with the sowing of flax seeds, local application of mineral fertilizers (nitroammophoska 2 c/ha) was carried out. The application of conservation tillage allows to obtain the yield of flax trust at the level of 3,5 t/ha, which is 0,4 t/ha (12.9 %) more than from the area of traditional tillage and 0,7 t/ha (25 %) in comparison with mulching. In the area with canning treatment, the seed yield was the highest and amounted to 0,64 t/ha. The difference between this option and traditional and mulching tillage reaches 0,06 t/ha (10,3 %) and 0.10 t/ha (18.5 %), respectively. Conclusions. Preservation tillage, which is based on shelf-free tillage to a depth of 40 cm and disking to a depth of 15 cm has a positive effect on plant growth and development, yield and quality of flax.


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