scholarly journals Comparative Proteomics at the Critical Node of Vigor Loss in Wheat Seeds Differing in Storability

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiuling Chen ◽  
Andreas Börner ◽  
Xia Xin ◽  
Manuela Nagel ◽  
Juanjuan He ◽  
...  
Keyword(s):  
Seed Viability ◽  
Storage Period ◽  
Protein Translation ◽  
Artificial Ageing ◽  
Plateau Phase ◽  
Cell Defense ◽  
Critical Node ◽  
Cytotoxic Compounds ◽  
Wheat Seeds

The critical node (CN, 85% germination) of seed viability is an important threshold for seed regeneration decisions after long-term conservation. Dependent on the germplasm, the storage period until CN is reached varies and information on the divergence of the proteomic profiles is limited. Therefore, the study aims to identify key proteins and mechanisms relevant for a long plateau phase and a late CN during artificial seed aging of wheat. Seeds of the storage-tolerant genotype (ST) TRI 23248, and the storage-sensitive genotype (SS) TRI 10230 were exposed to artificial ageing (AA) and extracted embryos of imbibed seeds were analyzed using an iTRAQ-based proteomic technique. ST and SS required AA for 24 and 18 days to reach the CN, respectively. Fifty-seven and 165 differentially abundant proteins (DAPs) were observed in the control and aged groups, respectively. Interestingly, a higher activity in metabolic processes, protein synthesis, transcription, cell growth/division, and signal transduction were already found in imbibed embryos of control ST seeds. After AA, 132 and 64 DAPs were accumulated in imbibed embryos of both aged ST and SS seeds, respectively, which were mainly associated with cell defense, rescue, and metabolism. Moreover, 78 DAPs of ST appeared before CN and were mainly enriched in biological pathways related to the maintenance of redox and carbon homeostasis and they presented a stronger protein translation ability. In contrast, in SS, only 3 DAPs appeared before CN and were enriched only in the structural constituents of the cytoskeleton. In conclusion, a longer span of plateau phase might be obtained in seeds when proteins indicate an intense stress response before CN and include the effective maintenance of cellular homeostasis, and avoidance of excess accumulation of cytotoxic compounds. Although key proteins, inherent factors and the precise regulatory mechanisms need to be further investigated, the found proteins may also have functional potential roles during long-term seed conservation.

scholarly journals Barley Seeds miRNome Stability during Long-Term Storage and Aging

2021 ◽  
Vol 22 (9) ◽  
pp. 4315
Author(s):  
Marta Puchta ◽  
Jolanta Groszyk ◽  
Magdalena Małecka ◽  
Marek D. Koter ◽  
Maciej Niedzielski ◽  
...  
Keyword(s):  
Seed Viability ◽  
Small Rna Sequencing ◽  
The Novel ◽  
Seed Aging ◽  
Long Term Storage ◽  
Novel Mirna ◽  
Complex Biological Process ◽  
Almost All ◽  
Term Storage

Seed aging is a complex biological process that has been attracting scientists’ attention for many years. High-throughput small RNA sequencing was applied to examine microRNAs contribution in barley seeds senescence. Unique samples of seeds that, despite having the same genetic makeup, differed in viability after over 45 years of storage in a dry state were investigated. In total, 61 known and 81 novel miRNA were identified in dry seeds. The highest level of expression was found in four conserved miRNA families, i.e., miR159, miR156, miR166, and miR168. However, the most astonishing result was the lack of significant differences in the level of almost all miRNAs in seed samples with significantly different viability. This result reveals that miRNAs in dry seeds are extremely stable. This is also the first identified RNA fraction that is not deteriorating along with the loss of seed viability. Moreover, the novel miRNA hvu-new41, with higher expression in seeds with the lowest viability as detected by RT-qPCR, has the potential to become an indicator of the decreasing viability of seeds during storage in a dry state.

scholarly journals Seed longevity of maize conserved under germplasm bank conditions for up to 60 years

2021 ◽  
Author(s):  
Filippo Guzzon ◽  
Maraeva Gianella ◽  
Jose Alejandro Velazquez Juarez ◽  
Cesar Sanchez Cano ◽  
Denise E Costich
Keyword(s):  
Seed Germination ◽  
Plant Genetic Resources ◽  
Seed Viability ◽  
Storage Conditions ◽  
Seed Longevity ◽  
Germplasm Bank ◽  
Significant Difference ◽  
Different Temperatures ◽  
Germination Experiments

Abstract Background and Aims The long-term conservation of seeds of plant genetic resources is of key importance for food security and preservation of agrobiodiversity. Nevertheless, there is scarce information available about seed longevity of many crops under germplasm bank conditions. Methods Through germination experiments as well as the analysis of historical monitoring data, we studied the decline in viability manifested by 1000 maize (Zea mays subsp. mays) seed accessions conserved for an average of 48 years at the CIMMYT germplasm bank, the largest maize seedbank in the world, under two cold storage conditions: an active (–3 °C; intended for seed distribution) and a base conservation chamber (–15 °C; for long-term conservation). Key Results Seed lots stored in the active chamber had a significantly lower and more variable seed germination, averaging 81.4 %, as compared with the seed lots conserved in the base chamber, averaging 92.1 %. The average seed viability detected in this study was higher in comparison with that found in other seed longevity studies on maize conserved under similar conditions. A significant difference was detected in seed germination and longevity estimates (e.g. p85 and p50) among accessions. Correlating seed longevity with seed traits and passport data, grain type showed the strongest correlation, with flint varieties being longer lived than floury and dent types. Conclusions The more rapid loss of seed viability detected in the active chamber suggests that the seed conservation approach, based on the storage of the same seed accessions in two chambers with different temperatures, might be counterproductive for overall long-term conservation and that base conditions should be applied in both. The significant differences detected in seed longevity among accessions underscores that different viability monitoring and regeneration intervals should be applied to groups of accessions showing different longevity profiles.

Ageing in embryos from wheat grains stored at different temperatures: oxidative stress and antioxidant response

2011 ◽  
Vol 38 (7) ◽  
pp. 624 ◽  
Author(s):  
Carmelina Spanò ◽  
Stefania Bottega ◽  
Roberto Lorenzi ◽  
Isa Grilli
Keyword(s):  
Oxidative Stress ◽  
Low Temperature ◽  
Seed Viability ◽  
Storage Period ◽  
Antioxidant Response ◽  
Protective Role ◽  
Native Page ◽  
Wheat Grains ◽  
Different Temperatures ◽  
Enzymatic Machinery

In the present work we studied oxidative stress as an important cause of seed deterioration during ageing in embryos from durum wheat grains stored at room temperature and at low temperature (10°C). The protective role of low temperature on seed viability was confirmed. The increase of hydrogen peroxide content during dry storage was strongly correlated with the decrease of germinability. Ascorbate and glutathione showed a good correlation with grain germinability and significantly increased upon imbibition, in particular in embryos from viable grains. Ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione peroxidase (GPX) and catalase (CAT) were studied quantitatively (enzymatic assays). APX, GR, and GPX were also studied qualitatively by native PAGE. The enzymes were active in dry, still viable, embryos whereas no activity was detected in non-viable embryos. With the exception of APX, all enzymatic activities decreased upon imbibition. The study of grains stored in different conditions indicated a negative correlation between the efficiency of the antioxidant enzymatic machinery and the age of the grain. The differences detected in differently stored materials confirmed that both germination parameters and the length of storage period are important in determining grain condition.

Effects of a long-term fire retardant chemical (Fire-Trol 934) on seed viability and germination of plants growing in a burned Mediterranean area

2007 ◽  
Vol 16 (3) ◽  
pp. 349 ◽  
Author(s):  
Belén Luna ◽  
José M. Moreno ◽  
Alberto Cruz ◽  
Federico Fernández-González
Keyword(s):  
Life Form ◽  
Seed Viability ◽  
Fire Retardant ◽  
Mediterranean Area ◽  
General Tendency ◽  
Progressive Decrease ◽  
Plant Groups ◽  
Distribution Ranges ◽  
High Concentrations

This work documents the effect of a common, long-term fire retardant chemical, Fire-Trol 934, on seed viability and germination of 36 plant species growing in a burned Mediterranean area, covering different life-form types, regenerative strategies and distribution ranges. Seeds were subjected to four treatments: control, and application of Fire-Trol 934 at concentrations of 0.02, 0.2 and 2%. Fire-Trol 934 significantly decreased both seed viability and germination in the group of species studied, which suggests that Fire-Trol 934 may be toxic for seeds, at least when applied at high concentrations. Whereas seed viability generally showed a progressive decrease with increased Fire-Trol 934 concentration, germination percentages generally increased when intermediate Fire-Trol 934 concentrations were used, but tended to be drastically reduced when seeds were exposed to the highest (2%) concentration. The reduction observed in germination at the highest Fire-Trol 934 concentration was greater than that observed in viability, which suggests that the effect of Fire-Trol 934 on seeds may not be lasting. Little differences in the response to Fire-Trol 934 emerged among plant groups, all of which followed the general tendency described above.

scholarly journals Biological features of seeds of a wild-growing species of Poterium polygamum

2022 ◽  
Vol 354 (11-12) ◽  
pp. 97-99
Author(s):  
M. A. Starostina ◽  
N. G. Lapenko
Keyword(s):  
Natural Population ◽  
Seed Viability ◽  
Storage Period ◽  
Seed Storage ◽  
Scientific Basis ◽  
The Third ◽  
Biological Features ◽  
High Germination ◽  
Grass Stand ◽  
The Subject

Relevance. Biological features (morphometric indicators, viability and germinating power) of Poterium polygamum seeds found in natural communites of the Stavropol Territory have been studied. The conservation of wild-growing plant species in the natural population and the rational use of their potential are relevant.Methods. Years of study — 2013–2021. The subject of the study is Poterium polygamum seeds. Seeds were collected in 2013. Studies were carried out according to the methods of studying seed material. Freshly harvested seeds and seeds of different shelf life were germinated in petri dishes at a temperature of 18–20 °C.Results. The collected seeds of Poterium polygamum are quite large. The length of the seeds is 4–5 mm, the width is about 2.5 mm. The mass of 1000 pieces of seeds on average was 10.59 grams. Complete absence of viability of freshly harvested Poterium polygamum seeds was revealed. After three months, seed viability was 97%. This is a shallow physiological rest associated with the post-harvest maturation period. During the storage of seeds, their high germination was established — from 70 to 100%. The duration of seed storage is 8 years. The largest number of germinated seeds and their germinating power were noted in the third year of their storage (100%). Seeds germinated on the third or fourth day. The differences between the viability of seeds and their germinating power are not great. In the eighth year of storage of Poterium polygamum seeds, viability and germinating power were slightly reduced. The storage period of seeds, in which they retain the ability to germinate and the germinating power, is long. It has been found that the wild species Poterium polygamum belongs to plants with a high (70 to 100%) viability. This species is regularly resumed in the natural population. The revealed biological features of Poterium polygamum seeds can become the scientific basis during the reconstruction of degraded natural grass stand and in selection work.

scholarly journals Mid storage seed hardening: a mechanical method to maintain seed viability during long term jute seed preservation

2021 ◽  
Vol 8 (1) ◽  
pp. 79-88
Author(s):  
Md. Nasir Uddin ◽  
S. M. Mahbub Ali ◽  
Md. Abu Sadat ◽  
Md Amazed Hossain Chowdhury ◽  
Israt Jahan Mumu ◽  
...  
Keyword(s):  
Crop Production ◽  
Agricultural Sector ◽  
Seed Viability ◽  
Early Period ◽  
Chemical Properties ◽  
Seed Storage ◽  
Germination Percentage ◽  
Oil Degradation ◽  
Hardening Treatment

Seed plays an important role in agricultural sector for both production and consumption purpose. Availability of vigour seed is one of the major constraints for maximizing crop production. However, healthy seed can also lose its viability during seed storage by changing different physio-chemical properties. Influence of environmental factors and seed containers during storage leading to seed deterioration. In this research, mid storage seed hardening treatment was applied in different aged seeds of jute species (C. Capsularis & C. olitorius) with two types of storage bags. Seed hardening treatment showed the less moisture content with better germination percentage compared to the untreated species of jute seeds. Seed packing in polythene bags during both short and long term seed storages had higher viable seeds compared to the cloth packing seeds. The effect of seed hardening treatment on seed oil content and pattern of oil degradation is distinct in early period of storage. The faster rate of oil degradation, soluble protein and free amino acids was found in seeds of un-treated stored seeds in cloth bag. Contrary, very slow rate of oil degradation was observed in harden seed and stored in polythene bag which indicated better storability of harden seeds.

Interaction of Epididymal Epithelia and their Secretions with Spermatozoa Supports Functional and Morphological Changes During Long-Term Storage in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

2020 ◽  
Vol 26 (3) ◽  
pp. 542-550
Author(s):  
Imran Tarique ◽  
Mansoor Tariq ◽  
Xuebing Bai ◽  
Qu Wenjia ◽  
Ping Yang ◽  
...  
Keyword(s):  
Electron Density ◽  
Morphological Changes ◽  
Storage Period ◽  
Sperm Storage ◽  
Synthetic Activity ◽  
High Electron ◽  
Pelodiscus Sinensis ◽  
Clear Cells ◽  
Long Term Storage

AbstractPost-testicular maturation of spermatozoa is crucial for attaining the morphological and functional capabilities needed for successful fertilization. Epididymal epithelia offer a favorable environment for spermatozoa that are stored long term in the turtle epididymis; however, sperm–epithelial interactions during storage, which are enormously important for sperm functional and morphological maturation, are still largely unknown in turtles. The present study examined the epididymis during the sperm-storage period (November–April) in the Chinese soft-shelled turtle (Pelodiscus sinensis). Light and transmission electron microscopy were used to determine the cellular features of each epididymal segment (caput, corpus, and cauda) and their epithelial interactions with the spermatozoa. Spermatozoa were mainly located in the lumena of caput, corpus, and cauda epididymides. Numerous spermatozoa were bound to apical surfaces of the epithelia, and several were even embedded in the epithelial cytoplasm of the caput and corpus epididymides. No embedded spermatozoa were found in the cauda epididymis. In all epididymal segments, principal and clear cells showed the synthetic activity, evidenced by a well-developed endoplasmic reticulum network and high and low electron-dense secretory materials, respectively. Principal and clear cells in the caput and corpus segments showed embedded spermatozoa in electron-dense secretions and in the lipid droplets within the cytoplasm. No lysosomes were observed around the embedded spermatozoa. The lumena of the caput and corpus segments showed few apocrine and low electron density secretions. In the lumen of the cauda epididymidis, different secretions, such as holocrine with low and high electron density and their fragmentation, apocrine, and dictyosome, were found and are summarized. Altogether, sperm physical interactions with secretions either in the cytoplasm of epithelium or in the lumen may support the viability, morphological maintenance, and transfer of various proteins involved in long-term sperm storage in the turtle. This interaction could help us to understand the mechanisms of long-term sperm storage and provide more insights into the reproductive strategies of turtle sperm preservation.

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