scholarly journals Morus alba L. Leaves – Integration of Their Transcriptome and Metabolomics Dataset: Investigating Potential Genes Involved in Flavonoid Biosynthesis at Different Harvest Times

2021 ◽  
Vol 12 ◽  
Author(s):  
Ding-Qiao Xu ◽  
Shu-Yan Cheng ◽  
Jun-Qing Zhang ◽  
Han-Feng Lin ◽  
Yan-Yan Chen ◽  
...  
Keyword(s):  
Animal Feed ◽  
Underlying Mechanism ◽  
Flavonoid Biosynthesis ◽  
Morus Alba ◽  
Mulberry Leaves ◽  
Mulberry Leaf ◽  
New Genes ◽  
Optimal Harvest ◽  
Significant Enrichment ◽  
Medicinal Properties

The mulberry leaf is a classic herb commonly used in traditional Chinese medicine. It has also been used as animal feed for livestock and its fruits have been made into a variety of food products. Traditionally, mulberry (Morus alba L.) leaf harvesting after frost is thought to have better medicinal properties, but the underlying mechanism remains largely unsolved. To elucidate the biological basis of mulberry leaves after frost, we first explored the content changes of various compounds in mulberry leaves at different harvest times. Significant enrichment of flavonoids was observed with a total of 224 differential metabolites after frost. Subsequently, we analyzed the transcriptomic data of mulberry leaves collected at different harvest times and successfully annotated 22,939 unigenes containing 1,695 new genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed 26, 20, and 59 unigenes related to flavonoids synthesis in three different groups harvested at different times. We found that the expression levels of flavonoid biosynthesis-related unigenes also increased when harvested at a delayed time, which was consistent with the flavonoid accumulation discovered by the metabolomic analysis. The results indicated that low temperature may be a key trigger in flavonoid biosynthesis of mulberry leaves by increasing the expression of flavonoid biosynthesis-related genes. This study also provided a theoretical basis for the optimal harvest time of mulberry leaves.

scholarly journals Effect of Morus alba leaf extract dose on lipid oxidation, microbiological stability, and sensory evaluation of functional liver pâtés during refrigerated storage

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260030
Author(s):  
Agnieszka Bilska
Keyword(s):  
Leaf Extract ◽  
Meat Products ◽  
Water Extract ◽  
Antioxidant Effect ◽  
Morus Alba ◽  
Oxidation Products ◽  
Mulberry Leaves ◽  
Mulberry Leaf ◽  
Natural Medicine ◽  
Mulberry Leaf Extract

Mulberry (Morus alba L.), and above all the extract from the leaves of this plant, is a natural medicine that has been used in traditional medicine for hundreds of years. Mulberry leaves contains polyphenol compounds: flavonoids, coumarins, numerous phenolic acids, as well as terpenes and steroids. The antioxidant effect of these compounds may be beneficial to the fat fraction of meat products, thereby increasing their functional qualities. The aim of the study was to evaluate the effectiveness of the use of mulberry water leaf extract, as an additive limiting adverse fat changes and affecting the functionality in model liver pâtés. Pork pâtés were prepared by replacing 20% of animal fat with rapeseed oil (RO), and water extract of mulberry leaves was added in the proportion of 0.2%, 0.6% and 1.0%. It has been shown that the addition of mulberry leaf extract delayed the appearance of primary and secondary fat oxidation products. The most effective antioxidant effect during 15-day storage was observed in the sample with the addition of 0.6% and 1.0% water mulberry leaf extract. These samples also showed inhibiting activity against angiotensin-converting enzymes and cholinesterase’s. During storage, the tested pâtés had a high sensory quality with unchanged microbiological quality. Mulberry leaf extract can be an interesting addition to the production of fat meat products, delaying adverse changes in the lipid fraction and increasing the functionality of products.

scholarly journals Foliar Spraying of 6-Benzylaminopurine Promotes Growth and Flavonoid Accumulation in Mulberry (Morus alba L.)

2021 ◽  
Author(s):  
Zhen Zhang ◽  
Yu Zhang ◽  
Shanying Zhang ◽  
Lifeng Wang ◽  
Xiaoyu Liang ◽  
...  
Keyword(s):  
Chalcone Synthase ◽  
Dietary Exposure ◽  
Morus Alba ◽  
Health Concern ◽  
Astragaloside Iv ◽  
Mulberry Leaves ◽  
Mulberry Leaf ◽  
Flavonoid Synthesis ◽  
Coa Ligase ◽  
Dietary Risk Assessment

AbstractMulberry (Morus alba L.) leaf, a “source of both medicine and food”, contains antioxidant ingredients such as flavonoids, alkaloids and polyphenols. The effects of 6-benzylaminopurine (6-BA) treatment on plant growth and flavonoid contents in mulberry leaves were investigated in this study. The expression of rutin (Rut), chlorogenic acid (ChA), isoquercitrin (IQ) and astragaloside IV (Ast) related genes in the flavonoid synthesis pathways was investigated in mulberry leaves. The results showed that 6-BA treatment significantly promoted mulberry differentiation and growth as well as, increased the numbers of new shoots and buds compared to the control. In addition, 30 mg/L 6-BA significantly increased the contents of Rut, IQ and Ast, and it strongly induced the expression of flavonoid biosynthesis-related genes, including flavonoid 3-O-glucosyltransferase (F3GT), 4-xoumarate-CoA ligase (4CL), phenylalanine (PAL) and chalcone synthase (CHS). The dietary risk assessment of mulberry leaves was based on hormone residues 5 days after treatment with 30 mg /L 6-BA, and the results showed that the dietary exposure risk of 6-BA was extremely low without causing any health concern. Thus, treatment with 30 mg/L 6-BA is a new method to improve the medicinal quality and development of high-value mulberry leaf foods without any potential risk.

scholarly journals ADDITION OF MULBERRY LEAF (MORUS ALBA L.) TO A DIET FORMULA IMPEDED ITS HYPOGLYCEMIC EFFECT AND EXACERBATED DYSLIPIDEMIA IN HIGH-FRUCTOSE- AND HIGH-FAT-INDUCED CD-1 MICE

2017 ◽  
pp. 532
Author(s):  
Xiaoxuan Guo ◽  
Jia Liu ◽  
Hongjuan Zhang ◽  
Baoping Ji ◽  
Feng Zhou
Keyword(s):  
Total Cholesterol Level ◽  
Fatty Acid Content ◽  
Morus Alba ◽  
Hypoglycemic Effect ◽  
Standard Diet ◽  
High Fat ◽  
Serum Free Fatty Acid ◽  
Mulberry Leaves ◽  
Mulberry Leaf ◽  
High Fructose

Backgrounds: Morus alba L. (mulberry leaf) is frequently used for therapeutic purposes in China. This study investigated the hypoglycemic and hypolipidemic effects of a reported antidiabetic herbal formula (PLCP) supplemented with mulberry leaves (MPLCP) in high-fructose- and high-fat- (HFF) fed mice. Methods: Six-week-old CD-1 male mice were fed on normal standard diet for 10 weeks. Fat emulsion with or without aqueous/ethanol extracts of PLCP/MPLCP was administrated by gavage daily for HFF groups. The normal group only received vehicle. 15% Fructose drinking water was supplied for HFF groups. Results: MPLCP was less effective than PLCP in hypoglycemic effect. The addition of mulberry leaves impeded not only the decrease in insulin resistance and serum free fatty acid content but also the increase in spleen index. The ethanol extract of MPLCP caused remarkable increase in low-density lipoprotein levels (p < 0.01) and significant increase in total cholesterol level (p < 0.05) compared with model group. Thus the effect of MPLCP was detrimental to abnormal serum lipid levels. Conclusion: The addition of mulberry leaves to PLCP weakened the hypoglycemic effects of the original formula and exacerbated dyslipidemia.

Flavonoids in seeds and grains: physiological function, agronomic importance and the genetics of biosynthesis

1998 ◽  
Vol 8 (4) ◽  
pp. 415-422 ◽  
Author(s):  
Brenda Winkel Shirley
Keyword(s):  
Secondary Metabolites ◽  
Condensed Tannins ◽  
Physiological Function ◽  
Animal Feed ◽  
Molecular Genetic ◽  
Flavonoid Biosynthesis ◽  
Human Consumption ◽  
Genetic Studies ◽  
Plant Seeds ◽  
Reproductive Structures

AbstractFlavonoids are secondary metabolites that are present at high levels in most plant seeds and grains. These compounds appear to play vital roles in defence against pathogens and predators and contribute to physiological functions such as seed maturation and dormancy. At the same time, particular subclasses of flavonoids, such as the proanthocyanidins (condensed tannins), negatively impact the use of seeds and grains in animal feed and can add undesirable qualities to food products for human consumption. This article presents an overview of investigations into the physiological and agronomic aspects of seed and grain flavonoids as well as a review of molecular genetic studies, particularly in maize,Arabidopsisand soybean, where mutants deficient in flavonoid biosynthesis provide useful tools for stydying the metabolic machinery underlying the accumulation of these compounds in reproductive structures.

scholarly journals URBAN ENVIRONMENTAL QUALITY ASSESSMENT BY SHAPE AND SPECTRAL INDICES OF MULBERRY LEAVES

2019 ◽  
Vol 7 (3) ◽  
pp. 184-205
Author(s):  
Snejana Dineva ◽  
Zlatin Zlatev
Keyword(s):  
Quality Assessment ◽  
Environmental Quality ◽  
Principal Components ◽  
Latent Variables ◽  
Support Vector ◽  
Mulberry Leaves ◽  
Environmental Quality Assessment ◽  
Mulberry Leaf ◽  
Vector Machines ◽  
Potential Use

In this paper, an analysis of the potential use of the surface and geometric characteristics of mulberry leaves as parameters for environmental quality assessment is made. Methods have been used to reduce the amount of data of latent variables, linear and kernel variants of principal components. It has been found that a kernel variant of the principal components, combined with nonlinear separating functions of discriminant analysis and a method of support vector machines, are an appropriate methods for distinguishing the degree of air pollution from the mulberry leaf data. The results obtained could be used as preliminary baseline data for future evaluations and studies related to remote monitoring of urban air quality.

scholarly journals Air-drying temperature changes the content of the phenolic acids and flavonols in white mulberry (Morus alba L.) leaves

2019 ◽  
Vol 49 (11) ◽  
Author(s):  
Monika Przeor ◽  
Ewa Flaczyk ◽  
Monika Beszterda ◽  
Krystyna Eleonora Szymandera-Buszka ◽  
Justyna Piechocka ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Phenolic Acid ◽  
Total Content ◽  
Morus Alba ◽  
Air Drying ◽  
Drying Temperature ◽  
Temperature Changes ◽  
Mulberry Leaves ◽  
White Mulberry ◽  
New Compounds

ABSTRACT: The white mulberry leaves are typically available on the market in dried or encapsulated form. It was assumed in the study that appropriate drying of leaves of the white mulberry is significant for obtaining intermediate products with high content of compounds having anti-oxidative activity. The purpose of the study was to determine the influence of the temperature of mulberry leaves air drying on the content of phenolic acids and flavonols. It has been determined that the content of these compounds in the leaves depended on the drying temperature. Drying at 60 °C favored release of phenolic acids and flavonols from complexes and/or formation of new compounds. Their total content was 22% higher than in leaves dried at 30 °C. Drying at 90 °C reduced the phenolic acid and flavonol content by 24%. The most favorable drying temperature was 60 °C.

PENGARUH ISOPROPIL MYRISTAT SEBAGAI BAHAN PENINGKAT PENETRASI TERHADAP LAJU DIFUSI KRIM PEMUTIH EKSTRAK ETANOL DAUN MURBEI (Morus alba L)

2017 ◽  
Vol 3 (1) ◽  
pp. 43
Author(s):  
Nurul Arfiyanti Yusuf ◽  
Aisyah Fatmawaty
Keyword(s):  
Leaf Extract ◽  
Diffusion Rate ◽  
Morus Alba ◽  
Penetration Enhancers ◽  
Mulberry Leaf ◽  
Mulberry Leaf Extract ◽  
Diffusion Studies ◽  
Before And After ◽  
Franz Diffusion Cells

The research has conducted research on the effectiveness of isopropyl myristat as a penetration enhancer on the diffusion rate of whitening cream mulberry leaf extract (Morus alba L) in vitro. This study aims to determine the effect of the use of isopropyl myristat. Mulberry leaf extract cream made with varying concentrations respectively 3%, 4%, 5% Isopropyl myristat as penetration enhancers made into 3 formulas (F1-F4) with the F1 without penetration enhancers. Evaluation of stability before and after accelerated storage includes observation of the organoleptic, emulsion type determination, measurement of pH, and viscosity. The evaluation results indicate four physically stable formula. In vitro diffusion studies conducted by Franz diffusion cells and footage is measured at a wavelength of 367.4 nm. The results of diffusion studies show that formula with the highest diffusion rate of 0.024 µg/minute on F4 (5% isopropyl myristat).

Spices

2022 ◽  
pp. 760-777
Author(s):  
Nisha ◽  
Deepika
Keyword(s):  
Blood Glucose ◽  
Experimental Evidence ◽  
Bioactive Compounds ◽  
Underlying Mechanism ◽  
Pharmacological Activities ◽  
Glucose Lowering ◽  
Scientific Methods ◽  
Human Trials ◽  
History Of ◽  
Medicinal Properties

The term “spices” has been derived from the word “species,” which was connected to the group of exotic foods in medieval times. Spices and herbs have a long history of culinary use, medicinal properties, and as additives and thus have a distinct place in Ayurveda. Exhibiting the merits of spices by scientific methods still remains a challenge. This review investigates the anti-diabetic properties in preventing and managing diabetics and associated complications with commonly used spices. The bioactive compounds in these spices are additionally discussed. The major aim and object of the present work is to investigate the customary therapeutic usage of basic Indian spices and to corelate their observed pharmacological activities with the presence of explicit bioactive compounds present for the treatment or counteractive action of diabetes. This includes the basic underlying mechanism of their blood glucose lowering property including exploratory experimental evidence from proposed animal and human trials.

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