scholarly journals ADDITION OF MULBERRY LEAF (MORUS ALBA L.) TO A DIET FORMULA IMPEDED ITS HYPOGLYCEMIC EFFECT AND EXACERBATED DYSLIPIDEMIA IN HIGH-FRUCTOSE- AND HIGH-FAT-INDUCED CD-1 MICE

2017 ◽  
pp. 532
Author(s):  
Xiaoxuan Guo ◽  
Jia Liu ◽  
Hongjuan Zhang ◽  
Baoping Ji ◽  
Feng Zhou
Keyword(s):  
Total Cholesterol Level ◽  
Fatty Acid Content ◽  
Morus Alba ◽  
Hypoglycemic Effect ◽  
Standard Diet ◽  
High Fat ◽  
Serum Free Fatty Acid ◽  
Mulberry Leaves ◽  
Mulberry Leaf ◽  
High Fructose

Backgrounds: Morus alba L. (mulberry leaf) is frequently used for therapeutic purposes in China. This study investigated the hypoglycemic and hypolipidemic effects of a reported antidiabetic herbal formula (PLCP) supplemented with mulberry leaves (MPLCP) in high-fructose- and high-fat- (HFF) fed mice. Methods: Six-week-old CD-1 male mice were fed on normal standard diet for 10 weeks. Fat emulsion with or without aqueous/ethanol extracts of PLCP/MPLCP was administrated by gavage daily for HFF groups. The normal group only received vehicle. 15% Fructose drinking water was supplied for HFF groups. Results: MPLCP was less effective than PLCP in hypoglycemic effect. The addition of mulberry leaves impeded not only the decrease in insulin resistance and serum free fatty acid content but also the increase in spleen index. The ethanol extract of MPLCP caused remarkable increase in low-density lipoprotein levels (p < 0.01) and significant increase in total cholesterol level (p < 0.05) compared with model group. Thus the effect of MPLCP was detrimental to abnormal serum lipid levels. Conclusion: The addition of mulberry leaves to PLCP weakened the hypoglycemic effects of the original formula and exacerbated dyslipidemia.

scholarly journals Gut Microbiota Induced by Pterostilbene and Resveratrol in High-Fat-High-Fructose Fed Rats: Putative Role in Steatohepatitis Onset

Nutrients ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 1738
Author(s):  
Iñaki Milton-Laskibar ◽  
Laura Judith Marcos-Zambrano ◽  
Saioa Gómez-Zorita ◽  
Alfredo Fernández-Quintela ◽  
Enrique Carrillo de Santa de Santa Pau ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Gut Microbiota ◽  
Metabolic Effects ◽  
Standard Diet ◽  
Protective Effects ◽  
Microbiota Composition ◽  
High Fat ◽  
Methoxy Derivative ◽  
High Fructose ◽  
Gut Microbiota Composition

Resveratrol and its 2-methoxy derivative pterostilbene are two phenolic compounds that occur in foodstuffs and feature hepato-protective effects. This study is devoted to analysing and comparing the metabolic effects of pterostilbene and resveratrol on gut microbiota composition in rats displaying NAFLD induced by a diet rich in saturated fat and fructose. The associations among changes induced by both phenolic compounds in liver status and those induced in gut microbiota composition were also analysed. For this purpose, fifty Wistar rats were distributed in five experimental groups: a group of animals fed a standard diet (CC group) and four additional groups fed a high-fat high-fructose diet alone (HFHF group) or supplemented with 15 or 30 mg/kg bw/d of pterostilbene (PT15 and PT30 groups, respectively) or 30 mg/kg bw/d of resveratrol (RSV30 group). The dramatic changes induced by high-fat high-fructose feeding in the gut microbiota were poorly ameliorated by pterostilbene or resveratrol. These results suggest that the specific changes in microbiota composition induced by pterostilbene (increased abundances of Akkermansia and Erysipelatoclostridium, and lowered abundance of Clostridum sensu stricto 1) may not entirely explain the putative preventive effects on steatohepatitis.

scholarly journals Effect of Morus alba leaf extract dose on lipid oxidation, microbiological stability, and sensory evaluation of functional liver pâtés during refrigerated storage

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260030
Author(s):  
Agnieszka Bilska
Keyword(s):  
Leaf Extract ◽  
Meat Products ◽  
Water Extract ◽  
Antioxidant Effect ◽  
Morus Alba ◽  
Oxidation Products ◽  
Mulberry Leaves ◽  
Mulberry Leaf ◽  
Natural Medicine ◽  
Mulberry Leaf Extract

Mulberry (Morus alba L.), and above all the extract from the leaves of this plant, is a natural medicine that has been used in traditional medicine for hundreds of years. Mulberry leaves contains polyphenol compounds: flavonoids, coumarins, numerous phenolic acids, as well as terpenes and steroids. The antioxidant effect of these compounds may be beneficial to the fat fraction of meat products, thereby increasing their functional qualities. The aim of the study was to evaluate the effectiveness of the use of mulberry water leaf extract, as an additive limiting adverse fat changes and affecting the functionality in model liver pâtés. Pork pâtés were prepared by replacing 20% of animal fat with rapeseed oil (RO), and water extract of mulberry leaves was added in the proportion of 0.2%, 0.6% and 1.0%. It has been shown that the addition of mulberry leaf extract delayed the appearance of primary and secondary fat oxidation products. The most effective antioxidant effect during 15-day storage was observed in the sample with the addition of 0.6% and 1.0% water mulberry leaf extract. These samples also showed inhibiting activity against angiotensin-converting enzymes and cholinesterase’s. During storage, the tested pâtés had a high sensory quality with unchanged microbiological quality. Mulberry leaf extract can be an interesting addition to the production of fat meat products, delaying adverse changes in the lipid fraction and increasing the functionality of products.

scholarly journals Nephroprotective effect of aqueous acetonic extract of Morus alba and its underlying mechanisms against glyphosate-induced toxicity - in vivo model

2021 ◽  
Vol 36 (1) ◽  
pp. 45-59
Author(s):  
Olfa Rebai ◽  
Sami Fattouch ◽  
Mohamed Amri
Keyword(s):  
Chlorogenic Acid ◽  
Protein Carbonyl ◽  
Morus Alba ◽  
In Vivo Model ◽  
Control Group ◽  
Standard Diet ◽  
Sod Activity ◽  
Mulberry Leaves ◽  
Polyphenolic Extract

Glyphosate, the active substance in RoundupR, is the most widely used pesticide in the world and may be present as a residue in derived foods and drinking water. Previous reports have confirmed that extracts from leaves of Morus alba exert many pharmacological activities. However, renoprotective effects of M. alba extract and its underling molecular mechanism is still unknown. Wistar rats (180-200 g) were used in this study (n=5-6). A control group received 0.2 ml normal saline intraperitoneally (i.p) once daily for two weeks. Control animals received standard diet. Treated groups received either polyphenolic extract (100 mg/kg,i.p) or glyphosate (100 mg/kg, i.p), or co-administration (extract ?g ml?1 kg b.w. and glyphosate 100 mg kg?1 b.w, i.p), daily until the 15thday of treatment. Lactate deshydrogenase LDH, serum concentrations of blood urea, creatinine and nitric oxide were measured using standard coloromertic methods. Renal oxidative stress, evidenced by increased malondialdehyde (MDA) and protein carbonyl levels and decline in superoxide dismutase (SOD) activity, was significantly alleviated by mulberry leaves extract (MLE) administration. MLE also appears to be able to modulate altered biochemical parametres by maintaining free iron and Ca2 + homeostasis, and regulate the endogenous antioxidant enzymes system. It seems that concurrent use of the aqueous acetonic fraction of M. alba, rich in chlorogenic acid and its isomeres, can protect kidneys from glyphosate-induced nephrotoxicity. Overall, MLE may possess protective activity against glyphosate-induced toxicity, which may be attributed to chlorogenic acid and its isomers, the most abundant phenolic acids present in its extracts. Mulberry leaves are a source of phenolic compounds and can be a good start towards discovering a new chemical compound which may lead to a new drug. A mulberry extract supplement could serve as a candidate for developing a safe, and promising nutraceutical product for the management of nephrotoxicity.

scholarly journals Comparative Effects of Pterostilbene and Its Parent Compound Resveratrol on Oxidative Stress and Inflammation in Steatohepatitis Induced by High-Fat High-Fructose Feeding

Antioxidants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1042
Author(s):  
Saioa Gómez-Zorita ◽  
Maitane González-Arceo ◽  
Jenifer Trepiana ◽  
Leixuri Aguirre ◽  
Ana B Crujeiras ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Parent Compound ◽  
Liver Steatosis ◽  
Standard Diet ◽  
High Fat ◽  
Alcoholic Fatty Liver ◽  
Expression Of Genes ◽  
High Fructose ◽  
Anti Oxidant ◽  
First Time

Different studies have revealed that oxidative stress and inflammation are crucial in NAFLD (Non-alcoholic fatty liver disease). The aim of this study is to analyze whether pterostilbene and resveratrol are able to either avoid or delay the progression of non-alcoholic liver steatosis towards steatohepatitis. This has been performed by examining their effects on oxidative stress, inflammation, fibrosis and pre-carcinogenic stages. Rats were distributed into five experimental groups and were fed with either a standard diet or a high-fat high-fructose diet, supplemented or not with pterostilbene (15 or 30 mg/kg/d) or resveratrol (30 mg/kg/d), for 8 weeks. Liver histological analysis was carried out by haematoxylin–eosin staining. Serum and hepatic oxidative stress-related parameters were assessed using spectrophotometry, and the expression of genes related to inflammation, fibrosis and cancer by qRT-PCR. The dietary model used in this study led to the development of steatohepatitis, where rats displayed oxidative stress, inflammation and ballooning, although not fibrosis. It also modified the expression of hepatocarcinoma-related genes. The results show, for the first time, that pterostilbene was able to partially prevent these alterations, with the exception of changes in hepatocarcinoma-related genes, mainly at 30 mg/kg/d. Pterostilbene was more effective than its parent compound resveratrol, probably due to its high bioavailability and higher anti-oxidant and anti-inflammatory activities, attributable to its different chemical structure.

scholarly journals Dietary Fresh and Boiled Mangkokan Leaves (Nothopanax Scutellarius) Normalized Body Weight, Serum Lipid Profile and Malondialdehyde in Metabolic Syndrome Rats

2020 ◽  
Vol 8 (3) ◽  
pp. 889-902
Author(s):  
Devi Elvina Rachma ◽  
Retno Murwani ◽  
Achmad Zulfa Juniarto
Keyword(s):  
Normal Control ◽  
Serum Lipid ◽  
Ldl Cholesterol ◽  
Hdl Cholesterol ◽  
Normal Control Group ◽  
Control Group ◽  
Standard Diet ◽  
High Fat ◽  
Cholesterol Levels ◽  
High Fructose

The antioxidant activity of Nothopanax scutellarius (Burm. f.) Merr, an edible plant, can prevent oxidative stress in metabolic syndrome (MetS). Thus, our research aimed to study the effect of dietary inclusion of fresh or boiled N. scutellarius on body weight and biochemical markers of Wistar rats with MetS. Twenty-four male Wistar rats were divided randomly into four groups, i.e., normal control group, high-fat-high-fructose diet (HFFD) group, fresh N. scutellarius (FNs) group, and boiled N. scutellarius (BNs) group. The normal control group was fed only a standard diet during the entire experiment. High-fat and high-fructose (HFHFr) diet accompanied with 20% fructose in drinking water to induce MetS was given to the HFFD, FNs, and BNs groups for 29 days. This was followed by a 29-day intervention diet in which standard normal diet, fresh N. scutellarius-containing standard diet, and boiled N. scutellarius-containing standard diet were given to the HFFD, FNs, and BNs groups, respectively. HFHFr diet significantly (p<0.05) raised fasting blood glucose (FBG), serum triglyceride, total cholesterol, LDL-cholesterol, and malondialdehyde (MDA), and significantly (p<0.05) reduced HDL-cholesterol. After 29 days on the intervention diet, serum triglycerides, total cholesterol, and LDL-cholesterol levels were found to decrease, and HDL-cholesterol levels were found to increase significantly (p<0.05). Thus, it can be concluded that dietary intake of N. scutellarius for 29 days can improve MetS components, i.e., FBG, serum lipid profile, and MDA, similar to those seen in rats on a normal control diet.

scholarly journals Exposure to maternal overnutrition and a high-fat diet during early postnatal development increases susceptibility to renal and metabolic injury later in life

2012 ◽  
Vol 302 (6) ◽  
pp. F774-F783 ◽  
Author(s):  
Colette M. Jackson ◽  
Barbara T. Alexander ◽  
Lauren Roach ◽  
Deani Haggerty ◽  
David C. Marbury ◽  
...  
Keyword(s):  
Postnatal Development ◽  
Normal Diet ◽  
Male Offspring ◽  
Standard Diet ◽  
High Fat ◽  
Metabolic Disturbances ◽  
Early Postnatal Development ◽  
High Fructose Diet ◽  
Urine Albumin ◽  
High Fructose

Overnutrition during pre- and postnatal development both confer increased susceptibility to renal and metabolic risks later in life; however, whether they have an additive effect on the severity of renal and metabolic injury remains unknown. The present study tested the hypothesis that a combination of a pre- and postnatal diet high in fat/fructose would exacerbate renal and metabolic injury in male offspring later in life. Male offspring born to high fat/high-fructose-fed mothers and fed a high-fat/high-fructose diet postnatally (HF-HF) had increased urine albumin excretion (450%), glomerulosclerosis (190%), and tubulointerstitial fibrosis (101%) compared with offspring born to mothers fed a standard diet and fed a standard diet postnatally (NF-NF). No changes in blood pressure or glomerular filtration were observed between any of the treatment groups. The HF-HF offspring weighed ∼23% more than offspring born to mothers fed a high-fat/high-fructose diet and fed a normal diet postnatally (HF-NF), as well as offspring born to mothers fed a standard diet regardless of their postnatal diet. The HF-HF rats also had increased (and more variable) blood glucose levels over 12 wk of being fed a high-fat/high-fructose diet. A combination of exposure to a high-fat/high-fructose diet in utero and postnatally increased plasma insulin levels by 140% compared with NF-NF offspring. Our data suggest that the combined exposure to overnutrition during fetal development and early postnatal development potentiate the susceptibility to renal and metabolic disturbances later in life.

scholarly journals Foliar Spraying of 6-Benzylaminopurine Promotes Growth and Flavonoid Accumulation in Mulberry (Morus alba L.)

2021 ◽  
Author(s):  
Zhen Zhang ◽  
Yu Zhang ◽  
Shanying Zhang ◽  
Lifeng Wang ◽  
Xiaoyu Liang ◽  
...  
Keyword(s):  
Chalcone Synthase ◽  
Dietary Exposure ◽  
Morus Alba ◽  
Health Concern ◽  
Astragaloside Iv ◽  
Mulberry Leaves ◽  
Mulberry Leaf ◽  
Flavonoid Synthesis ◽  
Coa Ligase ◽  
Dietary Risk Assessment

AbstractMulberry (Morus alba L.) leaf, a “source of both medicine and food”, contains antioxidant ingredients such as flavonoids, alkaloids and polyphenols. The effects of 6-benzylaminopurine (6-BA) treatment on plant growth and flavonoid contents in mulberry leaves were investigated in this study. The expression of rutin (Rut), chlorogenic acid (ChA), isoquercitrin (IQ) and astragaloside IV (Ast) related genes in the flavonoid synthesis pathways was investigated in mulberry leaves. The results showed that 6-BA treatment significantly promoted mulberry differentiation and growth as well as, increased the numbers of new shoots and buds compared to the control. In addition, 30 mg/L 6-BA significantly increased the contents of Rut, IQ and Ast, and it strongly induced the expression of flavonoid biosynthesis-related genes, including flavonoid 3-O-glucosyltransferase (F3GT), 4-xoumarate-CoA ligase (4CL), phenylalanine (PAL) and chalcone synthase (CHS). The dietary risk assessment of mulberry leaves was based on hormone residues 5 days after treatment with 30 mg /L 6-BA, and the results showed that the dietary exposure risk of 6-BA was extremely low without causing any health concern. Thus, treatment with 30 mg/L 6-BA is a new method to improve the medicinal quality and development of high-value mulberry leaf foods without any potential risk.

scholarly journals Effect of a high-fat–high-fructose diet, stress and cinnamon on central expression of genes related to immune system, hypothalamic–pituitary–adrenocortical axis function and cerebral plasticity in rats

2013 ◽  
Vol 111 (7) ◽  
pp. 1190-1201 ◽  
Author(s):  
Nathalie Marissal-Arvy ◽  
Cécile Batandier ◽  
Julien Dallennes ◽  
Frédéric Canini ◽  
Laurent Poulet ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Control Diet ◽  
Plasma Concentrations ◽  
Standard Diet ◽  
Immune Challenge ◽  
High Fat ◽  
Negative Effects ◽  
Cerebral Plasticity ◽  
High Fructose ◽  
Unbalanced Diet

The intake of a high-fat/high-fructose (HF/HFr) diet is described to be deleterious to cognitive performances, possibly via the induction of inflammatory factors. An excess of glucocorticoids is also known to exert negative effects on cerebral plasticity. In the present study, we assessed the effects of an unbalanced diet on circulating and central markers of inflammation and glucocorticoid activity, as well as their reversal by dietary cinnamon (CN) supplementation. A group of male Wistar rats were subjected to an immune challenge with acute lipopolysaccharide under a HF/HFr or a standard diet. Another group of Wistar rats were fed either a HF/HFr or a control diet for 12 weeks, with or without CN supplementation, and with or without restraint stress (Str) application before being killed. We evaluated the effects of such regimens on inflammation parameters in the periphery and brain and on the expression of actors of brain plasticity. To assess hypothalamic–pituitary–adrenocortical axis activity, we measured the plasma concentrations of corticosterone and the expression of central corticotrophin-releasing hormone, mineralocorticoid receptor, glucocorticoid receptor and 11β-hydroxysteroid dehydrogenase. We found that the HF/HFr diet induced the expression of cytokines in the brain, but only after an immune challenge. Furthermore, we observed the negative effects of Str on the plasma concentrations of corticosterone and neuroplasticity markers in rats fed the control diet but not in those fed the HF/HFr diet. Additionally, we found that CN supplementation exerted beneficial effects under the control diet, but that its effects were blunted or even reversed under the HF/HFr diet. CN supplementation could be beneficial under a standard diet, but deleterious under the unbalanced diet encountered in Western societies.

scholarly journals Etlingera elatior (Jack) R.M, Sm Containing Diet Normalizes some Metabolic Syndrome Markers due to High-Fat High-Fructose Diet in Wistar Rats

2020 ◽  
Vol 16 ◽  
Author(s):  
Nur Islami Dini Hanifah ◽  
Retno Murwani ◽  
Achmad Zulfa Juniarto
Keyword(s):  
Metabolic Syndrome ◽  
Body Weight ◽  
Total Cholesterol ◽  
Wistar Rats ◽  
Density Lipoprotein ◽  
Standard Diet ◽  
High Fat ◽  
High Fructose Diet ◽  
High Fructose ◽  
Etlingera Elatior

Background: Etlingera elatior (Ee) contains phytochemical compounds that are rich in antioxidants, which may reduce several biochemical markers of metabolic syndrome (MetS). Objective: We aimed to study the effect of fresh Etlingera elatior (FEe) and steamed Etlingera elatior (SEe) as a part of rat diet on the body weight, serum lipid, and malondialdehyde (MDA) level in Wistar rats with MetS induced by a highfat, high-fructose diet. Method: Our research was a true experimental randomized control group design with pre- and post-test. A total of 24 male Wistar rats were divided randomly into the following four groups: 1) Control, fed standard rat diet during the whole duration of the study, 2) HFFr-Sd, fed high-fat high-fructose (HFFr) diet for 29 days, followed by 29 days of the standard diet, 3) HFFr-FEe, fed HFFr diet for 29 days, followed by 29 days of a standard diet containing 33.3% FEe, and 4) HFFrSEe, fed HFFr diet for 29 days, followed by 29 days of a standard diet containing 33.3% SEe. The HFFr diet was given at 15 g/day along with fructose drink (20% pure fructose) at 100 ml/day. The diets in each group after the MetS induction period is referred to as intervention diets. Data at the end of HFFr (pre) and intervention diets (post) were analyzed by paired t-test. The data among groups were analyzed by one-way analysis of variance followed by post hoc test. Results: HFFr diet for 29 days induced MetS in Wistar rats fulfilling the criteria of obesity (Lee Index), hypertriglyceridemia, and decreased high-density lipoprotein cholesterol (HDL-C). Also, there was a significant increase in serum total cholesterol, low-density lipoprotein cholesterol (LDL-C), and MDA level (p < 0.05). Feeding a diet contaning FEe or SEe can significantly reduce body weight, serum triglyceride, total cholesterol, LDL-C, and MDA, and increase HDL-C levels (p < 0.05). The effect of FEe was more pronounced in ameliorating body weight and lipid profile than SEe. Conclusion: Fresh Ee and Steamed Ee can ameliorate obesity, dyslipidemia, and oxidative stress in MetS Wistar rats induced by a high-fat high-fructose diet. It suggests that dietary Ee accounting for one-third of daily standard diet can assist in normalizing some MetS markers in rats.

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