scholarly journals Metabolome and Transcriptome Reveal Novel Formation Mechanism of Early Mature Trait in Kiwifruit (Actinidia eriantha)

2021 ◽  
Vol 12 ◽  
Author(s):  
Guanglian Liao ◽  
Qing Liu ◽  
Xiaobiao Xu ◽  
Yanqun He ◽  
Yiqi Li ◽  
...  

Kiwifruit (Actinidia eriantha) is a peculiar berry resource in China, and the maturation period is generally late. Fortunately, we found an early mature A. eriantha germplasm. In order to explore the formation mechanism of its early mature trait, we determined the main carbohydrate and endogenous hormone content of the fruit, and used off-target metabolomics and transcriptomics to identify key regulatory metabolites and genes. We found that early mature germplasm had faster starch conversion rate and higher sucrose, glucose, and fructose content when harvested, while with lower auxin (IAA), abscisic acid (ABA), and zeatin (ZR) content. Through the non-targeted metabolome, 19 and 20 metabolites closely related to fruit maturity and early maturity were identified, respectively. At the same time, weighted correlation network analysis (WGCNA) showed that these metabolites were regulated by 73 and 99 genes, respectively, especially genes related to sugar metabolism were mostly. Based on above, the formation of early mature trait of A. eriantha was mainly due to the sucrose decomposition rate was reduced and the soluble solid content (SSC) accumulated at low levels of endogenous hormones, so as to reach the harvest standard earlier than the late mature germplasm. Finally, ten single nucleotide polymorphism (SNP) loci were developed which can be used for the identification of early mature trait of A. eriantha.

2014 ◽  
Vol 700 ◽  
pp. 364-367
Author(s):  
Hai Peng Lan ◽  
Yu Rong Tang ◽  
Jin Zhu Zhang ◽  
Yong Mei He

In order to investigate the mature law of Korla fragrant pear with mathematics method, Korla pears in Aksu area be taken as the study object, and the fruit hardness and soluble solid content (SSC) be selected as the research index. The data of the two indices in the mature process are analyzed with Excel, SigmaPlot and Matlab. To draw the change rule of indices and establish the mathematical mode. The research shows that the effective accumulated temperature reaching up 3000°C to 3843°C is main mature stage of the Korla fragrant pear, and there is an accelerating stage in which. The change rule can be precise description by the established mathematical model that can provide reference for fruit maturity modeling.


HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 505A-505
Author(s):  
Zhiguo Ju ◽  
Yousheng Duan ◽  
Zhiqiang Ju

`Feicheng' peach is a favorite cultivar in China due to is large size and high eating quality. However, its storage quality is poor and its market life is relatively short. Different combinations of AVG and GA3 applied at various stages of fruit development were evaluated to prolong market life of this fruit. A combination of 80-100 mg/L AVG and 80-100 mg/L GA3 at the end of pit hardening gave the best results. This treatment retarded the change in ground color, loss of firmness, and reduction in acidity by2 to 3 weeks. Since harvest was delayed, soluble solid content increased compared with the control that was harvested earlier. Fruit size increased significantly on treated trees. During 6 weeks of storage at 0 °C, ethylene evolution increased and fruit firmness decreased slowly in control fruit, but in AVG+GA3-treated fruit, they did not change from the low initial levels. At the end of storage, control fruit developed a high percentage (83%) of tissue browning and mealiness after warming at 20 °C for 4 days, but the AVG+GA3-treated fruit ripened normally and developed much less (16%) tissue browning and mealiness. Our results showed that the market life of `Feicheng' peaches can be prolonged by at least 4 weeks by using the AVG+GA3 treatment to delay harvest and improve storage quality.


2019 ◽  
Vol 14 (7) ◽  
pp. 649-657 ◽  
Author(s):  
Li Li ◽  
Jiemin Li ◽  
Jian Sun ◽  
Ping Yi ◽  
Changbao Li ◽  
...  

Background: Phospholipase D (PLD)is closely related to browning and senescence of postharvest longan fruit. Objective: This study investigated the effects of 2-butanol (a PLD inhibitor) on the expression and regulation of PLD during storage of longan fruit at a low temperature. Methods: Senescence-related quality indices showed that the 2-butanol-treated fruit presented lower pericarp browning index, pulp breakdown index and total soluble solid value than the untreated fruit. Results: The fruit treated by 60 µL/L 2-butanol exhibited the strongest inhibition on senescence, which significantly delayed changes in weight, titratable acidity content, total soluble solid content and ascorbic acid content. This treatment maintained a high level of total phenolic content and caused significant inhibition on pericarp browning and pulp breakdown. Through ELISA method, 60 µL/L 2-butanol treatment also reduced PLD activity. Real-time RT-PCR (RT-qPCR) results showed that PLD mRNA expression level was inhibited by 60 µL/L 2-butanol within 15 days. Western-blotting results further confirmed the differential expression of PLD during storage, and a relatively higher expression for PLD protein was found in control compared to the 2-butanoltreated fruit during 15-d storage. Conclusion: These results provided a scientific basis and reference to further investigating postharvest longan quality maintenance by regulating the PLD gene expression.


Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 449
Author(s):  
Camilo Gutiérrez-Jara ◽  
Cristina Bilbao-Sainz ◽  
Tara McHugh ◽  
Bor-Sen Chiou ◽  
Tina Williams ◽  
...  

The cracking of sweet cherries causes significant crop losses. Sweet cherries (cv. Bing) were coated by electro-spraying with an edible nanoemulsion (NE) of alginate and soybean oil with or without a CaCl2 cross-linker to reduce cracking. Coated sweet cherries were stored at 4 °C for 28 d. The barrier and fruit quality properties and nutritional values of the coated cherries were evaluated and compared with those of uncoated sweet cherries. Sweet cherries coated with NE + CaCl2 increased cracking tolerance by 53% and increased firmness. However, coated sweet cherries exhibited a 10% increase in water loss after 28 d due to decreased resistance to water vapor transfer. Coated sweet cherries showed a higher soluble solid content, titratable acidity, antioxidant capacity, and total soluble phenolic content compared with uncoated sweet cherries. Therefore, the use of the NE + CaCl2 coating on sweet cherries can help reduce cracking and maintain their postharvest quality.


2016 ◽  
Vol 11 (3) ◽  
pp. 203 ◽  
Author(s):  
Luigi Morra ◽  
Maurizio Bilotto ◽  
Domenico Cerrato ◽  
Raffaella Coppola ◽  
Vincenzo Leone ◽  
...  

Two trials in different agricultural farms were carried out from October 2014 to June 2015 with the aim to assess the advantages linked to the substitution of the low density polyethylene (LDPE) films for soil mulching with the Mater-Bi<sup>®</sup> biodegradable films in the strawberry cultivation under tunnel in Campania. Lifetime of biodegradable mulch and influence of type of mulch on the yield and the quality of cvs Sabrina and Fortuna were evaluated. Plants were cultivated on mulched, raised beds, high 40 cm from bottom soil. Mater-Bi<sup>®</sup> film was 20 <span>µ</span>m thick while LDPE film was 50 mm thick. The physical-chemical parameters (firmness, pH, total soluble solid content, titratable acidity and skin colour) and some bioactive compounds (total polyphenols, flavonoids, anthocyanins, antioxidant activity) of fruits were determined by three samplings effected in consecutive months (from March to May 2015) of the harvest cycle. Biodegradable film guaranteed an effective mulch along the whole strawberry cycle (9-10 months including the time of drawing up of film). Yields of cv Sabrina on LDPE was 18% higher than those on Mater-Bi<sup>®</sup> while the opposite was detected in cv Fortuna (+10%). The physical-chemical parameters of fruits were not modified by the mulches. The content of the bioactive compounds, instead, resulted, in each time of sampling, significantly higher in fruits picked on Mater-Bi<sup>®</sup> based film.


2020 ◽  
Vol 115 (2) ◽  
pp. 297
Author(s):  
Seda TUNÇAY ÇAĞATAY ◽  
Gülşah ÇALIK KOÇ ◽  
Fereshteh REZAEİ ◽  
Özlem DARCANSOY İŞERI ◽  
Feride İffet ŞAHIN ◽  
...  

<p>This study aimed to investigate the effects of grafting tomato on different tobacco rootstocks on quality factors and nicotine content. The commercial variety (Solanum lycopersicum ‘H2274’) (BIOTECH) of the tomato was used as the scion plant, and six different tobacco (Nicotiana tabacum L.) rootstocks were used: Taşova, Tekel, Muş, Samsun, Dişbudak, Hasankeyf cultivars. Cleft grafting method was used in all plants. Yield of non-grafted and grafted plants grown in open-field conditions was calculated, and there was a significant increase in yield in grafted tomatoes compared to non-grafted tomatoes. There was significantly increased lycopene and β-carotene levels (mg kg-1), especially in ‘Tekel’, ‘Taşova’, ‘Samsun’, and ‘Hasankeyf’ tobacco grafts. There was a statistically significant difference between grafted and non-grafted plants according to 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical retention capacities and total phenol (TP) values. Evaluation of quality determinants including pH values, titratable acidity values (citric acid %), soluble solid content (SSC)(oBrix) , fruit size ratios, showed that tomatoes grafted with ‘Muş’ tobacco rootstock were of higher quality. There was no significant difference between grafted and non-grafted plants according to nicotine analysis of the tobacco-grafted tomatoes, and due to acceptable ranges of nicotin level on tobacco grafted tomato plants were considered to be suitable for consumption. It could be concluded that grafting practices have significantly positive effects on tomato yield and quality.<br /><br /></p>


Author(s):  
Kamile Ulukapı ◽  
Zehra Kurt ◽  
Sevinc Sener

Arbuscular mycorrhizal fungi (AMF), which are beneficial soil organisms, have an important role in the uptake of plant nutrients by roots and thus help to healthy plant growth. The aim of this study was to determine the effects of AMF inoculation on the development of water-deficiency applied pepper plants. In this study, Tesla F1 pepper cultivars, Glomus etunicatum inoculated and without Glomus etunicatum, were exposed to four different irrigation regimes (25I, 50I, 75I, 100I). At the end of the experiment these plants were compared in terms of some vegetative and fruit properties. For this purpose, at the end of the trial; shoot length (cm), root length (cm), root spread (cm), number of leaves, leaf width and length (mm), stem diameter (mm), fruit width (mm), fruit length (mm), root and shoot weights (g), fruit pH, total soluble solid content and chlorophyll index were measured. P (phosphorus) and K (potassium) contents of leaves samples taken from plants were determined. As a result, it was determined that 75I irrigation regime gave the best results in terms of both plant growth and fruit properties in all mycorrhizal and non-mycorrhizal plants. It was also concluded that 75% irrigation level is sufficient for plant growth.


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