scholarly journals Combined Use of Vinasse and Nitrogen as Fertilizers Affects Nitrification, Ammonification, and Denitrification by Prokaryotes

2021 ◽  
Vol 1 ◽  
Author(s):  
Miriam Gonçalves de Chaves ◽  
Andressa Monteiro Venturini ◽  
Luis Fernando Merloti ◽  
Dayane Juliate Barros ◽  
Raffaella Rossetto ◽  
...  

A common agricultural practice of combining organic fertilizer vinasse (a liquid residue from sugarcane ethanol production) with mineral nitrogen (N) fertilizer promotes N losses such as greenhouse gas emissions due to the effects of physicochemical changes in soil on the microbiota inhabiting this environment. In this study, we applied microarray GeoChip v.5.0M technology to obtain a better insight into the prokaryotic communities and identify and quantify the N functional gene families associated with the N processes in sugarcane soils without N fertilizer (N0), with urea at 60 kg ha−1 (N60), and with vinasse combined with urea (NV). Soil samples were collected at 7 (T7) and 150 (T150) days after N application, corresponding to maximum and minimum nitrous oxide (N2O) emissions, respectively, for molecular and physicochemical analysis. Additionally, the metagenomes of these DNA samples, previously deposited in the MG-RAST server, were accessed to investigate the functions and taxonomic groups associated with selected gene families. The results revealed that 87% of the select gene families were significantly responsive to the fertilizer combined treatment (NV) in the 7 days after the application. The most responsive genes and processes were nitrification [with the amoA gene from ammonia-oxidizing Bacteria (AOB) and Archaea (AOA) and hao from Bacteria], ammonification (with gdh and ureC genes from Bacteria and Archaea), and denitrification (with p450nor from Eukarya). The AOA, Nitrosopumilus, and AOB, Nitrosomonas, were the groups with the greatest functions associated with nitrification, as well as a pathogenic Mycobacterium, with denitrification. The results also revealed that under N fertilizers and decreased O2 in soil, the increases in K and P nutrients can promote the growth of the halophile Archaea Natronomonas and the Bacteria Anaeromyxobacter, which can reduce N2O. In conclusion, this typical agricultural fertilization management may favor functional genes and archaeal and bacterial groups associated with N processes that have the potential to reduce environmental damage in tropical sugarcane soils.

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaoxiao Shu ◽  
Yanqun Wang ◽  
Yaling Wang ◽  
Yang Ma ◽  
Mingxin Men ◽  
...  

AbstractThe appropriate nitrogen (N) fertilizer regulator could increase N utilization of crops and reduce N losses in the North China Plain. We investigated the effects of reduced inorganic-N rate combined with an organic fertilizer on nitrous oxide (N2O) emissions in winter wheat and summer maize rotation system. Simultaneously studied the effect of different treatments on N use efficiency (NUE), N balance and net income. After reducing the amount of nitrogen fertilizer in the wheat-corn rotation system, the results showed that the cumulative emission of soil N2O from the RN40% + HOM [40% of RN (recommended inorganic-N rate) with homemade organic matter] treatment was 41.0% lower than that of the RN treatment. In addition, the N production efficiency, agronomic efficiency, and apparent utilization were significantly increased by 50.2%, 72.4% and 19.5% than RN, respectively. The use of RN40% + HOM resulted in 22.0 and 30.1% lower soil N residual and N losses as compared with RN. After adding organic substances, soil N2O cumulative emission of RN40% + HOM treatment decreased by 20.9% than that of the HAN (zinc and humic acid urea at the same inorganic-N rate of RN) treatment. The N production efficiency, N agronomic efficiency and NUE of RN40% + HOM treatment were 36.6%, 40.9% and 15.3% higher than HAN’s. Moreover, soil residual and apparent loss N were 23.3% and 18.0% less than HAN’s. The RN40% + HOM treatment appears to be the most effective as a fertilizer control method where it reduced N fertilizer input and its loss to the environment and provided the highest grain yield.


2011 ◽  
Vol 8 (11) ◽  
pp. 3159-3168 ◽  
Author(s):  
Y. Xia ◽  
X. Yan

Abstract. Nitrogen (N) fertilizer plays an important role in agricultural systems in terms of food yield. However, N application rates (NARs) are often overestimated over the rice (Oryza sativa L.) growing season in the Taihu Lake region of China. This is largely because negative externalities are not entirely included when evaluating economically-optimal nitrogen rate (EONR), such as only individual N losses are taken into account, or the inventory flows of reactive N have been limited solely to the farming process when evaluating environmental and economic effects of N fertilizer. This study integrates important material and energy flows resulting from N use into a rice agricultural inventory that constitutes the hub of the life-cycle assessment (LCA) method. An economic evaluation is used to determine an environmental and economic NAR for the Taihu Lake region. The analysis reveals that production and exploitation processes consume the largest proportion of resources, accounting for 77.2 % and 22.3 % of total resources, respectively. Regarding environmental impact, global warming creates the highest cost with contributions stemming mostly from fertilizer production and farming processes. Farming process incurs the biggest environmental impact of the three environmental impact categories considered, whereas transportation has a much smaller effect. When taking account of resource consumption and environmental cost, the marginal benefit of 1 kg rice would decrease from 2.4 to only 1.05 yuan. Accordingly, our current EONR has been evaluated at 187 kg N ha−1 for a single rice-growing season. This could enhance profitability, as well as reduce the N losses associated with rice growing.


2021 ◽  
Vol 34 (4) ◽  
pp. 824-829
Author(s):  
CAMILA SENO NASCIMENTO ◽  
CAROLINA SENO NASCIMENTO ◽  
ARTHUR BERNARDES CECÍLIO FILHO

ABSTRACT Splitting nitrogen (N) fertilizer application can be an efficient nutrient management technique to improve productivity and plant quality, as well as to reduce the negative environmental impact caused by N losses. In this context, the present study investigated how the management of N affects the agronomic characteristics of field-grown arugula plants. Nine treatments were assessed in a randomized complete block design, in a 4 x 2 + 1 factorial scheme, with three replicates. The evaluated factors were doses of N (60, 120, 180 and 240 kg N ha-1), split N fertilizer applications at side-dress (two and three times) and an additional treatment without a N supply. Maximum height was obtained with the application of 198 kg N ha-1. Nitrate content, fresh mass and productivity increased with increasing N doses. There was no effect of split N fertilizer applications on the characteristics evaluated. Therefore, the supply of 240 kg N ha-1 divided into two portions was considered as the best management strategy.


2019 ◽  
Vol 5 (2) ◽  
pp. 131
Author(s):  
Muh Yusuf Indris ◽  
Irwan Halid ◽  
Sukriming Sapareng

This study aims to determine the effectiveness of N2-fixing microbes contained in organic organic fertilizers at four levels of nitrogen fertilizer application in increasing the performance of oil palm seedlings. The experiment was carried out in the form of an experiment using a Randomized Block Design with 2 factors: organic organic fertilizer and nitrogen fertilizer. The first factor with three levels of treatment, namely without organic biological fertilizer, Organic fertilizer from cow dung (Agro Flower) 1 g / kg of Soil and Organic organic fertilizer (Biost) 1 g / kg of Soil. While the second factor consists of four levels, namely without N fertilizer, N fertilizer 50% of the standard dose, N fertilizer 100% of the standard dose, and N fertilizer of 150% of the standard dose. Each treatment was tested on 4 oil palm seedlings and repeated 3 times, so that 144 experimental plants were obtained. The observations made were the variable plant performance includes plant height, number of leaves, and stem diameter. Variants of plant nutrient levels included total N nutrient levels, P nutrient levels, and K nutrient levels. The results showed that N-inhibiting microbes contained in organic organic fertilizer increased plant height and stem diameter significantly in oil palm nurseries. The use of a 100% dose of N fertilizer together with organic biofertilizers shows the best crop performance results.


1994 ◽  
Vol 119 (3) ◽  
pp. 427-433 ◽  
Author(s):  
C.A. Sanchez ◽  
R.L. Roth ◽  
B.R. Gardner

Six field studies were conducted from 1980-88 to evaluate the response of cabbage (Brassica oleracea L., Capitata group) to sprinkler irrigation and sprinkler-applied N fertilizer on a coarse-textured soil. The plots were irrigated using a modified self-moving lateral sprinkler irrigation system that applied five levels of water and five levels of N (liquid NH4NO3) in specified combinations of central composite rotatable design. Cabbage yields were significantly increased by water and N applications in all experiments. The N rates predicted for maximum yield exceeded typical cabbage N fertilizer recommendations. However, the above-average plant populations used in these studies resulted in above-average yields and plant N accumulation. Deficit and excess irrigation produced negative results. Generally, cabbage production was optimized and N losses to the environment were minimized when crops were irrigated for evapotranspiration (ET) replacement. However, even when irrigated for ET replacement, these data demonstrate the potential for N leaching at high N rates, presumably as a result of rainfall.


Author(s):  
Sergii Digtiar ◽  
Alyona Pasenko ◽  
Olha Novokhatko ◽  
Oksana Maznytska ◽  
Olena Nykyforova

Due to the istabiity of market prices for traditional hydrocarbon energy resources and negative impact of their combustion products on the environment, recently quite rapidly develop technology that provide for, first of all, renewable energy sources. One of the promising areas of alternative energy is bioenergy is a branch of biotechnology that considers organic matter of biomass of various origins (mainly agricultural waste) as a fuel source. Among the main advantages of energy biotechnology, it should be noted the speed and relative ease of recovery of the energy substrate, as well as the fact that it is not alien to the environment, so, even if released in quantities exceeding the permissible ones, this will not lead to irreversible changes in the ecosystem. Excess biomass will quickly be incorporated into biogeochemical cycles, which is the key to minimizing environmental damage. The process of bioconversion of methane from organic substrate compounds (carbohydrates, proteins, nucleic acids, lipids, organic acids, alcohols), which in three stages, is called methanogenesis. This process involves a specific complex bacterial enzyme system, consisting of the following coenzymes: methanofuran, tetrahydro-methanopterin, coenzymes F420 and F430, coenzyme M (CoM), coenzyme B. Using of ways of processing and waste disposal, that based on alternative biological recycling methods of organic pollutants does not always allow to achieve the expected effect, in particular on artificially created ecosystems of animal farms working in a closed mode. Concentration on a small area of a significant amount of biowaste (manure, dung, process runoffs etc.) of the cattle kept in the facilities of live-stock industry, contaminates soils, water bodies and atmospheric air with microorganisms, dust, harmful gases and other decomposition products. Often, it is one of the main factors constrainting on the growth of livestock enterprises and agro-industrial complex (AIC) capacities in general, restraining the economic development of regions specializing in agricultural production. For the first time in the region, study was carried out in order to determine the most efficient way to process miskantus phytomass for the production as target products of methane-containing biogas mixture and organo-mineral fertilizer suitable for use in agriculture and forestry. An economically and ecologically attractive way of utilization of wastes of phyto- and zoogenic origin, which makes it possible significantly reduce anthropogenic pressure on the environment, has been proposed.


2016 ◽  
Vol 6 (1) ◽  
pp. 17
Author(s):  
INDAH PERMANASARI ◽  
AULIA RANI ANNISAVA

Utilization of organic material in crop farming is one of the methods to exploit the local wisdom in agricultural practice. The research was conducted from June to September 2012 in the research farm, State Islamic University of Sultan Syarif Kasim Riau. The objective of the research was to investigate the effects of organic materials and crop density ? in growth and yield on cucumber. Randomized block design with 2 factors and 4 replications was employed in this study. The first factor consists of three levels organic fertilizer treatments i.e. no fertilizer, poultry manure, and compost of oil palm empty bunch. Second factor comprises of three levels of crop density i.e. 1, 2, and 3 plants per pot).. The results showed that there was significant increase on growth and yield on cucumber with poultry manure treatment compare to that of with compost of oil palm empty bunch. Increasing crop density ? improves the number and weight of cucumber yield per pot, even though, some parameters, i.e. number of leaves, stem diameter, crop dry weight, productive number of branches, cucumber size and cucumber weight per plant were significantly decreased.


Author(s):  
Bernou Zoë van der Wiel ◽  
Jan Weijma ◽  
Corina Everarda van Middelaar ◽  
Matthias Kleinke ◽  
Cees Jan Nico Buisman ◽  
...  

AbstractRegions with intensive agriculture often encounter environmental problems caused by nutrient excess of agro-food-waste systems that have become increasingly linear over previous decades. In this study, nitrogen (N), phosphorus (P), potassium (K) and carbon (C) flows in the whole agro-food-waste system of district Cleves in Germany were quantified simultaneously using substance flow analysis. Moreover, nutrient use inefficiency hotspots were identified to establish options to improve nutrient self-sufficiency as a first step towards nutrient circularity. Data on mass flows and nutrient contents was acquired for the year 2016 from stakeholders, statistical databases, literature and modelling. Organic C was included for flows with potential as organic fertilizer. Results show that animal production drives the nutrient flows in the export-oriented district, with feed import, manure application and losses from housing and manure storage accounting for 40, 45 and 60% of all N, P and K flows, respectively. In particular agriculture is responsible for N losses, with 150 kg N lost ha−1 agricultural land. Crop production surplus and with that soil accumulation of P and K are 515 t and 4100 t respectively. Stoichiometry of N:P:K:C in the different organic materials does not allow direct application and meeting crop requirements without exceeding demand of especially P. Processing of biomass is therefore required. Based on mass, especially manure holds potential for processing into bio-based fertilizers. To improve nutrient cycling and soil C conservation, being an important element for a sustainable agricultural sector, local balances between crop and animal production need to be considered.


2010 ◽  
Vol 20 (2) ◽  
pp. 304-307 ◽  
Author(s):  
George E. Boyhan ◽  
Ray J. Hicks ◽  
Reid L. Torrance ◽  
Cliff M. Riner ◽  
C. Randell Hill

In a 3-year study of poultry litter applications on short-day onion (Allium cepa) production, where rates ranged from 0 to 10 tons/acre, there was an increasing linear effect on total onion yield. Jumbo (≥3 inches diameter) onion yield did not differ with increasing poultry application rates, while medium (≥2 and <3 inches diameter) yields decreased with increasing applications of poultry litter. In addition, organic-compliant fertilizers, 4N–0.9P–2.5K at 150 to 250 lb/acre nitrogen (N), as well as 13N–0P–0K at 150 lb/acre N and in combination with 9N–0P–7.5K totaling 150 lb/acre N were evaluated. Comparison of these commercial organic-compliant fertilizers indicated that there were no differences in total or jumbo yields, while medium yields generally decreased with increased N fertilizer rate.


2019 ◽  
Vol 99 (2) ◽  
pp. 117-125 ◽  
Author(s):  
Isabelle Perron ◽  
Athyna N. Cambouris ◽  
Bernie J. Zebarth ◽  
Philippe Rochette ◽  
Noura Ziadi

Large inputs of nitrogen (N) are required to optimize yield and quality of potato (Solanum tuberosum L.), and it may result in a high potential for N losses including denitrification. This 5 yr study compared the effect of three N fertilizer sources [ammonium nitrate (AN), ammonium sulfate (AS), and polymer-coated urea (PCU)] at 200 kg N ha−1 (N200) and an unfertilized control (N0) on denitrification rate (DR) from irrigated potato production on a coarse-textured soil in eastern Canada. Fertilizer was banded all at-planting (PCU) or split 40% at-planting and 60% at-hilling (AN and AS). The DR was measured biweekly from planting to harvest at two locations (ridge and furrow) using the acetylene blockage technique. The mean annual DR, averaged across N treatments, ranged from 0.8 to 8.0 μg N2O-N kg dry soil−1 d−1, and it was most closely related to the water inputs in the 72 h before DR measurements. Mean DR averaged across year was greater for N200 than for N0 (4.2 vs. 3.4 μg N2O-N kg dry soil−1 d−1) but did not differ among N sources. Our results suggest that choice of N fertilizer source in sandy soils is more important in controlling losses of N by leaching than by denitrification.


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