scholarly journals Impact of Cryopreservation on Spermatozoa Freeze-Thawed Traits and Relevance OMICS to Assess Sperm Cryo-Tolerance in Farm Animals

2021 ◽  
Vol 8 ◽  
Author(s):  
Ibrar Muhammad Khan ◽  
Zubing Cao ◽  
Hongyu Liu ◽  
Adnan Khan ◽  
Sajid Ur Rahman ◽  
...  
Keyword(s):  
Animal Species ◽  
Damage Mechanism ◽  
Farm Animals ◽  
Sperm Cryopreservation ◽  
Omics Technologies ◽  
Molecular Alterations ◽  
Livestock Breeding ◽  
Specific Proteins ◽  
Mammalian Spermatozoa

Sperm cryopreservation is a powerful tool for the livestock breeding program. Several technical attempts have been made to enhance the efficiency of spermatozoa cryopreservation in different farm animal species. However, it is well-recognized that mammalian spermatozoa are susceptible to cryo-injury caused by cryopreservation processes. Moreover, the factors leading to cryo-injuries are complicated, and the cryo-damage mechanism has not been methodically explained until now, which directly influences the quality of frozen–thawed spermatozoa. Currently, the various OMICS technologies in sperm cryo-biology have been conducted, particularly proteomics and transcriptomics studies. It has contributed while exploring the molecular alterations caused by cryopreservation, identification of various freezability markers and specific proteins that could be added to semen diluents before cryopreservation to improve sperm cryo-survival. Therefore, understanding the cryo-injury mechanism of spermatozoa is essential for the optimization of current cryopreservation processes. Recently, the application of newly-emerged proteomics and transcriptomics technologies to study the effects of cryopreservation on sperm is becoming a hotspot. This review detailed an updated overview of OMICS elements involved in sperm cryo-tolerance and freeze-thawed quality. While also detailed a mechanism of sperm cryo-injury and utilizing OMICS technology that assesses the sperm freezability potential biomarkers as well as the accurate classification between the excellent and poor freezer breeding candidate.

scholarly journals Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

2020 ◽  
Vol 21 (8) ◽  
pp. 2781 ◽  
Author(s):  
Patricia Peris-Frau ◽  
Ana Josefa Soler ◽  
María Iniesta-Cuerda ◽  
Alicia Martín-Maestro ◽  
Irene Sánchez-Ajofrín ◽  
...  
Keyword(s):  
Molecular Basis ◽  
Molecular Mechanisms ◽  
Sperm Quality ◽  
Sperm Cryopreservation ◽  
Molecular Changes ◽  
Omics Technologies ◽  
Ruminant Species ◽  
Livestock Breeding ◽  
Molecular Aspects ◽  
Sperm Freezing

Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm cryopreservation in different ruminant species. However, a significant amount of sperm still suffers considerable cryodamage, which may affect sperm quality and fertility. Recently, the use of different “omics” technologies in sperm cryobiology, especially proteomics studies, has led to a better understanding of the molecular modifications induced by sperm cryopreservation, facilitating the identification of different freezability biomarkers and certain proteins that can be added before cryopreservation to enhance sperm cryosurvival. This review provides an updated overview of the molecular mechanisms involved in sperm cryodamage, which are in part responsible for the structural, functional and fertility changes observed in frozen–thawed ruminant sperm. Moreover, the molecular basis of those factors that can affect the sperm freezing resilience of different ruminant species is also discussed as well as the molecular aspects of those novel strategies that have been developed to reduce sperm cryodamage, including new cryoprotectants, antioxidants, proteins, nanoparticles and vitrification.

scholarly journals Current perspectives on ruminant sperm freezability: Harnessing molecular changes related to semen quality through omics technologies

2021 ◽  
Vol 19 (3) ◽  
pp. 487-511
Author(s):  
Marvin Bryan Salinas ◽  
◽  
Phongsakorn Chuammitri ◽  
Korawan Sringarm ◽  
Sukolrat Boonyayatra ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Assisted Reproductive Technologies ◽  
Semen Quality ◽  
Reproductive Technologies ◽  
Reproductive Efficiency ◽  
Molecular Tools ◽  
Sperm Cryopreservation ◽  
Omics Technologies ◽  
Molecular Bases

The recent advances in sperm cryopreservation transcend cryobanking and other assisted reproductive technologies. Since its discovery, cryopreservation has contributed positive impacts on animal breeding as well as in genetic exchange, improvement, and conservation efforts. However, cryoinjury and variabilities in cryopreservation outcomes remain as key challenges to sperm cryobiology. The present work explored the molecular bases for such freezability differences and freezing-thawing injuries in the ruminant sperm. Relevant biomarkers identified in the seminal plasma and the spermatozoa were highlighted, including lipids, proteins, metabolites, transcripts, and genes. Specific molecular mechanisms concerning sperm structures and functions were also examined relative to their association to cryotolerance, and spermiogram or seminogram modifications following cryopreservation procedures. Current conflicts and gaps in the knowledge base on ruminant spermatozoa were also emphasized. Further investigation of these areas using the available breakthrough molecular tools such as omics technologies is therefore proposed to improve, optimize, or even predict the overall quality of frozen-thawed ruminant semen towards reproductive efficiency.

Registration of animal rabies in Krasnodar region

2020 ◽  
pp. 3-4
Author(s):  
Oleg Yu. Chernykh ◽  
◽  
Vadim A. Bobrov ◽  
Sergey N. Zabashta ◽  
Roman A. Krivonos ◽  
...  
Keyword(s):  
Urban Form ◽  
Animal Species ◽  
Farm Animals ◽  
Natural Focus ◽  
Dead End ◽  
Wide Range ◽  
Epizootic Process ◽  
Krasnodar Region ◽  
Spread Of Infection ◽  
Reporting Data

Rabies remains a constant threat to humanity in many parts of the world. At the same time, scientifically grounded antiepizootic measures should be based on the peculiarities of the regional epizootology of this zooanthroponosis. The authors studied the epizootological and statistical reporting data of the Kropotkin Regional Veterinary Laboratory, presented an analysis of the registration of rabies in animals in Krasnodar region. From the obtained data, it should be noted that despite the wide range of animals involved in the epizootic process of rabies infection in Krasnodar region, dogs, cats and foxes play a major role in the reservation and spread of infection, which account for 78.6. Of the total number of registered cases, 15.5% falls on foxes, that indicates the natural focus of the disease, along with the manifestation of the disease in an urban form. At the same time, stray and neglected dogs and cats, which occupy a significant place among the total number of sick animals, are also sources and spread of the infection. Thus farm animals (8.3% of the total number of infected animals) are a biological dead end for the infection. Isolated cases of the disease were noted in muskrat, donkey, raccoon, raccoon dog, marten, ferret and jackal. The authors also established the specific morbidity of various animal species with rabies infection, that is an important aspect in the development and implementation of antiepizootic measures complex

STRUCTURAL AND LOGISTIC MODEL OF MATERIAL FLOWS ON DIGITALLIVESTOCK FARM

2020 ◽  
pp. 26-32
Author(s):  
VLADIMIR V KIRSANOV VLADIMIR V ◽  
Keyword(s):  
Functional Model ◽  
Base Stations ◽  
Farm Animals ◽  
Biological Objects ◽  
Livestock Breeding ◽  
Video Images ◽  
Genetic Potential ◽  
Common Control ◽  
Biotechnical System ◽  
Multi Agent

The problems of the digitalization of livestock enterprises are closely related to the construction of models and algorithms describing the functioning of individual technological processes and subsystems united by a common control system. Based on the cluster approach, three groups of tasks for the intellectualization and digitalization of objects in livestock breeding are formulated: 1) recognition of images of biological objects and models of their group and individual behavior, 2) genomic assessment of farm animals, prediction of their genetic potential, with the possibility of better adaptation to technologies and specifi c economic conditions, 3) multi-agent management of automated and robotic technical means. The authors initialized the video images of biological objects, developed a structural and functional model of a complex biotechnical system “Man-Machine-Animal”, including automated workstations of key specialists, signal receiving-and-transmitting base stations, technological modules for animal service (feeding, watering, milking, microclimate, etc.), representing local biotechnical systems. The paper presents a structural-and-logistic “funnel” model of a livestock farm functioning. The model includes vectors of incoming material fl ows, outgoing production fl ows and outgoing byproducts (production waste) described using appropriate formalizations. The authors provide the structural typifi cation of technological modules and subsystems for their mathematical analysis and subsequent digital transformation of livestock farms.

scholarly journals The Quality and Health-Promoting Value of Meat from Pigs of the Native Breed as the Effect of Extensive Feeding with Acorns

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 789
Author(s):  
Magdalena Szyndler-Nędza ◽  
Małgorzata Świątkiewicz ◽  
Łukasz Migdał ◽  
Władysław Migdał
Keyword(s):  
Fatty Acid ◽  
Beneficial Effect ◽  
Meat Quality ◽  
Farm Animals ◽  
Feeding Method ◽  
Native Breed ◽  
The World ◽  
Health Promoting ◽  
Conventional Farm

The popularity of meat from animals of native breeds is growing all over the world, due to consumer belief regarding its higher quality compared to meat from industrial farm animals. In addition, the living conditions (welfare) are of great importance for consumers. We observed the effect of different ways of keeping and feeding pigs of the same conservative breed on the quality of meat and its health benefits. The aim of the study was to compare the meat quality from pigs of the native Złotnicka Spotted breed, fattened intensively or extensively (with conventional farm-produced compound feed and acorns). The meat from free-range pigs extensively fed on silage and small amounts of acorns was characterized by a higher content of fat, which acts as a carrier for flavor and juiciness, as well as higher monounsaturated fatty acid (MUFA) content (p ≤ 0.05) and lower atherogenic, thrombogenic, and peroxidability indices (p ≤ 0.05). It may be stated that the meat quality of the native pig breed is significantly dependent on the housing and feeding method. A more beneficial effect on the quality of meat and its dietetic value, as well as its susceptibility to rancidity, can be obtained throughextensive pig feeding with roughage and the addition of acorns.

Chromosomal instability in different species of agricultural animals (review)

2021 ◽  
pp. 19-26
Author(s):  
V. A. Andreeva
Keyword(s):  
Chromosomal Instability ◽  
Animal Species ◽  
Western Siberia ◽  
Reproductive Function ◽  
Farm Animals ◽  
Chromosomal Disorders ◽  
Important Indicator ◽  
Karyotypic Analysis ◽  
Different Types ◽  
Almost All

The purpose of the work was to analyze the frequency of chromosomal instability in different species of agricultural animals in Western Siberia on the basis of literature data. The analysis of the literature on the topic of somatic chromosomal instability in agricultural animals has been carried out. Despite the stability of the chromosome set, deviations from it are quite common. It is noteworthy that chromosomal instability is characteristic of almost all individuals in the population and serves as an important indicator for assessing the natural mutability of chromosomes. It has been found if an aberration appeared in one tissue, it is very likely to occur in others. It has been noted that somatic chromosomal instability occurs in animals with reduced reproductive function, as well as those suffering from any pathology. For example, in calves with parakeratosis the increase in the frequency of chromatid and isochromatid breaks has been found. Analysis of the frequency of aberrations in sires, which differ in the level of perinatal mortality of offspring revealed the increased percentage of offspring mortality in fathers with the large number of chromosomal disorders. Therefore, low fertilization, spontaneous abortions and stillbirths may be indications for karyotypic analysis. It has been revealed that the lability of the karyotype is inherent in all animal species, regardless of the species, sex and age, as well as the morphofunctional state. The data on some types of somatic chromosomal instability in different types of farm animals in Western Siberia has been presented. Such indicators as the frequency of polyploidy, the number of cells with fragments of chromosomes, as well as single and paired fragments of chromosomes has been given. Different types of chromosomal instability varied depending on the animal species and climate zone. The presented data can be accepted as a physiological norm and used in veterinary medicine and animal science.

scholarly journals Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

2015 ◽  
Vol 75 (3) ◽  
pp. 662-669 ◽  
Author(s):  
EG Sanches ◽  
IR Oliveira ◽  
PCS Serralheiro ◽  
VR Cerqueira
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Equilibration Time ◽  
Dilution Ratio ◽  
Sperm Cryopreservation ◽  
Ph Values ◽  
Cryopreserved Sperm ◽  
Time Density ◽  
Motility Rate

AbstractThis study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C –min), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C –min, 1 minute of equilibration time and 1:3 (v/v) dilution ratio. The use of cryopreserved sperm presented fertilization rates >60% validating the present protocol for lane snapper. The cryoconserved sperm of lane snapper is a viable alternative, being possible to maintain appropriate sperm viability.

scholarly journals Application of automated bioacoustic identification in environmental education and assessment

2004 ◽  
Vol 76 (2) ◽  
pp. 446-451 ◽  
Author(s):  
Teruyo Oba
Keyword(s):  
Environmental Education ◽  
Environmental Monitoring ◽  
Computer Science ◽  
Animal Species ◽  
Biological Surveys ◽  
The Future ◽  
Technology Aids

Developments in electronics and computer science have led to the introduction of an automated bioacoustic identification device used to resolve commonly encountered problems in the identification of animal species in the field. This technology aids our auditory observations, and also improves the quality of biological surveys and environmental monitoring. In this paper the future roles and possibilities of bioacoustics are discussed, providing some examples from the realm of environmental education and monitoring that focus on the use of nature sounds.

scholarly journals Features of parasitocenosis in eimeriosis in young ruminants

2021 ◽  
Vol 0 (14) ◽  
pp. 91-100
Author(s):  
Vera Usevich ◽  
N. Kurochkina ◽  
M. Drozd ◽  
Ol'ga Petrova
Keyword(s):  
Comparative Evaluation ◽  
Animal Species ◽  
Treatment Time ◽  
Global Scale ◽  
Laboratory Diagnostics ◽  
Therapeutic Effectiveness ◽  
Treatment And Prevention ◽  
Traditional Remedies ◽  
Eimeria Arloingi

Abstract. The prevalence of coccidiosis diseases of various animal species has a global scale, so this problem has attracted the attention of veterinary specialists around the world. The purpose of this study was to study the specific features of the species composition and laboratory diagnostics of eimeriosis in young ruminants and to evaluate the effectiveness of treatment and prevention when using drugs from different groups. The material for the study was goats and calves. The research was carried out on the basis of two farms of the same agricultural holding in the Sverdlovsk region. Diagnostics was performed based on clinical and laboratory studies. Results of the study. The main pathogens in the goat breeding complex in goats of different ages were identified: the main causative agent of coccidiosis in 120-day-old goats was Eimeria arloingi, which accounted for 54 % of all detected oocysts in the samples. Eimeriosis occurred as a mixed invasion of Eimeria arloingi with Eimeria ninaekohlyakimovae (33 %) and Eimeria intracata (13 %). Eimeria arloingi (68 %), in 21-days-old goats – Eimeria ninaekohlyakimovae (32 %). Eimeriosis caused by Eimeria bovis was detected in 30-days-old calves (100 %). The article describes the results of using various anticoccidial drugs in the treatment and prevention of eimeriosis. A comparative evaluation of the therapeutic effectiveness of known coccidiostatics is given. The economic efficiency of different groups of drugs was evaluated. The scientific novelty lies in the fact that the morphological and quantitative assessment of parasitocenoses in eimeriosis in young ruminants was carried out. A comparative evaluation of the effectiveness of various traditional remedies and a new drug for the treatment of eimeriosis in calves and goats was carried out. Conclusion. Not all of the drugs used have the same therapeutic effectiveness both in terms of treatment time and the quality of elimination of the etiological factor.

scholarly journals R-Score: A New Parameter to Assess the Quality of Variants’ Calls Assessed by NGS Using Liquid Biopsies

Biology ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 954
Author(s):  
Roberto Serna-Blasco ◽  
Estela Sánchez-Herrero ◽  
María Berrocal Renedo ◽  
Silvia Calabuig-Fariñas ◽  
Miguel Ángel Molina-Vila ◽  
...  
Keyword(s):  
Egfr Mutation ◽  
False Negative ◽  
Digital Pcr ◽  
Liquid Biopsies ◽  
Molecular Alterations ◽  
Significant Linear Correlation ◽  
Molecular Landscape ◽  
Nsclc Patients ◽  
Next Generation Sequencing Ngs

Next-generation sequencing (NGS) has enabled a deeper knowledge of the molecular landscape in non-small cell lung cancer (NSCLC), identifying a growing number of targetable molecular alterations in key genes. However, NGS profiling of liquid biopsies risk for false positive and false negative calls and parameters assessing the quality of NGS calls remains lacking. In this study, we have evaluated the positive percent agreement (PPA) between NGS and digital PCR calls when assessing EGFR mutation status using 85 plasma samples from 82 EGFR-positive NSCLC patients. According to our data, variant allele fraction (VAF) was significantly lower in discordant calls and the median of the absolute values of all pairwise differences (MAPD) was significantly higher in discordant calls (p < 0.001 in both cases). Based on these results, we propose a new parameter that integrates both variables, named R-score. Next, we sought to evaluate the PPA for EGFR mutation calls between two independent NGS platforms using a subset of 40 samples from the same cohort. Remarkably, there was a significant linear correlation between the PPA and the R-score (r = 0.97; p < 0.001). Specifically, the PPA of samples with an R-score ≤ −1.25 was 95.83%, whereas PPA falls to 81.63% in samples with R-score ≤ 0.25. In conclusion, R-score significantly correlates with PPA and can assist laboratory medicine specialists and data scientists to select reliable variants detected by NGS.

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