scholarly journals Extracts of Emmer Wheatgrass Grown with Distilled Water, Salinity or Selenium Differently Affect Germination and Cytosolic Ca2+ of Maize Pollen

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 633
Author(s):  
Alberto Marco Del Pino ◽  
Beatrice Falcinelli ◽  
Roberto D’Amato ◽  
Daniela Businelli ◽  
Paolo Benincasa ◽  
...  

In this work, the biological activity of emmer (Triticum turgidum L. spp. dicoccum (Schrank ex Shubler) Thell.) wheatgrass extracts obtained from grains sprouted with distilled water, or salinity (50 mM) or selenium (45 mg L−1 of Na2SeO3), was tested through an experimental biological model based on the germination and cytosolic Ca2+ homeostasis of maize pollen grains. The effects of thapsigargin (TG) and of four phenolic acids (PAs: ferulic, coumaric, salicylic and 3-HO benzoic) on maize pollen were also tested as controls. Wheatgrass extracts influenced both pollen cytosolic Ca2+ and germination. The Ca2+ agonist activity of emmer wheatgrass was transient, different from that of TG, which caused a depletion of the stored Ca2+ and a permanent alteration of Ca2+ homeostasis. The results obtained with extracts compared to those obtained with pure PAs suggest that PAs in unconjugated forms, which are known to be well represent in emmer wheatgrass, contribute to the biological activity of extracts. The extent of the biological response of emmer wheatgrass extracts was influenced by emmer sprouting conditions (i.e., distilled water, or salinity or selenium). Maize pollen treated with Se-enriched wheatgrass extracts showed a less perturbed cytosolic Ca2+ and a higher germination rate.

2014 ◽  
Vol 955-959 ◽  
pp. 2123-2127
Author(s):  
Rong Yang ◽  
Jia Ping Li ◽  
Yan Zhang

Use different concentrations of biological activity water on corn seeds (Zhen Dan 958) by soaking to deal with, dilution multiple is primary liquid、10 times、50 times、100 times、200 times、400 times and 800 times respectively, as a comparison with distilled water, exploring the influence of biological activity water on corn seeds germination and seedling growth. The result shows: the highest germination rate、germination potential、germination index and germination energy of corn seeds are dealt with by dilute 800 times biological activity water, the aboveground part and root of maze seedlings’ dry weight is largest; dealing with by dilute 800 times biological activity water of maze seedlings’ chlorophyll are at its highest. The experiment tells that biological activity water has effect on corn seeds germination and seedling growth, its effect and dilute strength are related.


Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 32
Author(s):  
Hosakatte Niranjana Murthy ◽  
Guggalada Govardhana Yadav ◽  
Yaser Hassan Dewir ◽  
Abdullah Ibrahim

Many underutilized tree species are good sources of food, fodder and possible therapeutic agents. Balanites aegyptiaca (L.) Delile belongs to the Zygophyllaceae family and is popularly known as “desert date”, reflecting its edible fruits. This tree grows naturally in Africa, the Middle East and the Indian subcontinent. Local inhabitants use fruits, leaves, roots, stem and root bark of the species for the treatment of various ailments. Several research studies demonstrate that extracts and phytochemicals isolated from desert date display antioxidant, anticancer, antidiabetic, anti-inflammatory, antimicrobial, hepatoprotective and molluscicidal activities. Mesocarp of fruits, seeds, leaves, stem and root bark are rich sources of saponins. These tissues are also rich in phenolic acids, flavonoids, coumarins, alkaloids and polysterols. Some constituents show antioxidant, anticancer and antidiabetic properties. The objective of this review is to summarize studies on diverse bioactive compounds and the beneficial properties of B. aegyptiaca.


2016 ◽  
Vol 11 (12) ◽  
pp. 1040-1047 ◽  
Author(s):  
Albino Corazza Kaefer Kaian ◽  
Chiapetti Ricardo ◽  
Foga ccedil a Luciana ◽  
Luis Muller Alexandre ◽  
Borghetti Calixto Guilherme ◽  
...  
Keyword(s):  

2015 ◽  
Vol 43 (1) ◽  
pp. 153-158 ◽  
Author(s):  
Faruk TOKLU

An experiment was conducted under laboratory and field conditions in order to evaluate the effects of different priming treatments, specifically KNO3 (1%), KCl (2%), KH2PO4 (1%), ZnSO4 (0.05%), PEG-6000 (20%), IBA (100 ppm), Mannitol (4%), GA3 (100 ppm) and distilled water, on seed germination properties and several agro-morphological plant characteristics of red lentil. Seeds not primed were used as a control. GA3 treatment increased shoot length. The control (non-primed seeds) treatment resulted in increased seedling root number and length. Distilled water, ZnSO4 and control treatments increased germination rate and percentage. In the pot experiments, GA3 treatment increased plant height and seedling emergence rate, whereas KCl treatment improved the number of nodules, as well as root and shoot dry weight when compared to the control. ZnSO4 treatment increased yield components and grain yield in field conditions. The results of this study showed that ZnSO4, GA3 and PEG-6000 seed priming treatments may be useful tools due to their positive effects on germination rate, germination percentage, yield component and grain yield of lentil.


F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 2040
Author(s):  
Ricardo Vivas-Reyes ◽  
Alejando Morales-Bayuelo ◽  
Carlos Gueto ◽  
Juan C. Drosos ◽  
Johana Márquez Lázaro ◽  
...  

Background: Heat shock protein (Hsp90KDa) is a molecular chaperone involved in the process of cellular oncogenesis, hence its importance as a therapeutic target in clinical trials. Geldanamycin is an inhibitor of Hsp90 chaperone activity, which binds to the ATP binding site in the N-terminal domain of Hsp90. However, geldanamycin has shown hepatotoxic damage in clinical trials; for this reason, its use is not recommended. Taking advantage that geldanamycin binds successfully to Hsp90, many efforts have focused on the search for similar analogues, which have the same or better biological response and reduce the side effects of its predecessor; 17-AAG and 17-DMAG are examples of these analogues. Methods: In order to know the chemical factors influencing the growth or decay of the biological activity of geldanamycin analogues, different computational techniques such as docking, 3DQSAR and quantum similarity were used.  Moreover, the study quantified the interaction energy between amino acids residues of active side and geldanamycin analogues, through hybrid methodologies and density functional theory (DFT) indexes. Results: The evaluation of interaction energies showed that the interaction with Lys58 residue is essential for the union of the analogues to the active site of Hsp90, and improves its biological activity. This union is formed through a substituent on C-11 of the geldanamycin macrocycle. A small and attractor group was found as the main steric and electrostatic characteristic that substituents on C11 need in order to interact with Lys 58; behavior was observed with hydroxy and methoxy series of geldanamycin analogues, under study. Conclusion: These outcomes were supported with quantum similarity and reactivity indices calculations using DFT in order to understand the non-covalent stabilization in the active site of these compounds.


2021 ◽  
Vol 7 (3) ◽  
pp. 64-69
Author(s):  
L. Bayramov

Abstract. The zones of distribution of varieties and forms of quince on the territory of the Nakhchivan Autonomous Republic have been established, phenological observations have been carried out, their flowering and fruiting have been studied. On the territory of the Autonomous Republic, flowering of varieties and forms of quince begins in the second decade of April, depending on the distribution zone, with an average daily temperature of 12–13 °C and lasts 12–13 days, depending on weather conditions. Each flower has 10–12 stamens arranged in one row. The article also studied the viability of pollen in a number of quince varieties. Pollen viability was studied in the varieties Sary, Tursh, Ordubad, Gara and wild forms. Pollen fertility was determined by staining with acetocarmine. Pollen germinates in 2–5–10–15 and 20% glucose solution. Counting of germinated pollen grains was carried out under a microscope. The study showed that of all the experimental varieties, the pollen fertility of the Sary quince and Tursh quince varieties is high (up to 96.6–97.1%). The best medium for the germination of quince pollen is a 10–15% glucose solution. Pollen germination in this solution reaches 47.4–88.0%. In distilled water (control), the germination of quince pollen reached from 9.7% to 35.6% for varieties. Quince pollen remains viable for 31–43 days.


2021 ◽  
Author(s):  
Mohammed Alghazal ◽  
◽  
Dimitrios Krinis ◽  

Fluid saturation data obtained from core analysis are used as control points for log calibration, saturation modeling and sweep evaluation. These lab-derived data are often viewed as ground-truth values without fundamentally understanding the key limitations of experimental procedures or scrutinizing the accuracy of measured lab data. This paper presents a unique assessment of sponge core data through parameterization, uncertainty analysis and Monte-Carlo modeling of critical variables influencing lab-derived saturation results. This work examines typical lab data and reservoir information that could impact final saturation results in sponge coring. We dissected and analyzed ranges of standard raw data from Dean-Stark and spectrometric analysis (including, gravimetric weights, distilled water volumes, pore volumes and sponge’s absorbance), input variables of fluid and rock properties (such as, water salinity, formation volume factors, plug’s dimension and stress corrections), governing equations (including, salt correction factors, water density correlations and lab mass balance equations) and other factors (for instance, sources of water salinity, filtrate invasion, bleeding by gas liberation and water evaporation). Based on our investigation, we have identified and statistically parameterized 11 key variables to quantify the uncertainty in lab-derived fluid saturation data in sponge cores. The variables’ uncertainties were mapped into continuous distributions and randomly sampled by Monte-Carlo simulation to generate probabilistic saturation models for sponge cores. Simulation results indicate the significance of the water salinity parameter in mixed salinity environments, ranging between 20,000 to 150,000 ppm. This varied range of water salinity produces a wide uncertainty spectrum of core oil saturation in the range of +/- 3 to 10% saturation unit. Consequently, we developed two unique salinity variance models to capture the water salinity effect and minimize the uncertainty in the calculation of core saturation. The first model uses a material balance to solve for the salinity given the distilled water volume and gravimetric weight difference of the sample before and after leaching. The second model iteratively estimates the salinity required to achieve 100% of total fluids saturation at reservoir condition after correcting for the bleeding, stress and water evaporation effects. Our work shows that these derived models of water salinity are consistent with water salinity data from surface and bottom-hole samples. Despite the prominence of applications of core saturation data in many aspects of the industry, thorough investigation into its quality and accuracy is usually overlooked. To the best of our knowledge, this is the first paper to present a novel analysis of the uncertainty coupled with Monte-Carlo simulation of lab-derived saturation’s data from sponge cores. The modeling approach and results highlighted in this work provide the fundamental framework for modern uncertainty assessment of core data.


2011 ◽  
Vol 57 (No. 2) ◽  
pp. 52-56 ◽  
Author(s):  
E. Jafariehyazdi ◽  
F. Javidfar

Members of Brassicaceae have been frequently cited as allelopathic crop. The toxic effect of Brassica spp. may be caused by hydrolysis products of glucosinolates that occur in substantial amounts in the vegetative parts of Brassica spp. This study investigated the allelopathic potential of Brassica napus, B. rapa and B. juncea on the sunflower seed germination and seedling growth. Aqueous extracts of three species from two stages (full flowering and straw) of sampling were separately made with 0 (distilled water), 10, 20, 30 and 40% concentrations. This experiment was conducted in 2 × 3 × 5 factorial arrangement based on completely randomized design with five replications. There was a highly significant difference among different concentrations of extracts and also between two stages of extraction. All aqueous extracts significantly affected sunflower germination, germination rate, seedling root and hypocotyl length, fresh and dry matter weight when compared with distilled water control. The greatest concentration showed a stronger inhibitory effect. Root length was more sensitive to extracts than hypocotyl length.


2012 ◽  
Vol 60 (4) ◽  
pp. 345-355 ◽  
Author(s):  
M. Jamil ◽  
M. Ashraf ◽  
E. Rha

Salinity reduces plant growth and yield by affecting morphological and physiological processes. To alleviate the harmful effects of salt stress various approaches involving plant hormones are used. In this study several parameters involving the measurement of cell membrane injury were used to observe whether stress tolerance could be enhanced in Chinese cabbage (B. oleracea capitata L. Chinensis group) by soaking the seeds for 10 h in distilled water (control), or in 100, 150 or 200 mg l−1 gibberellic acid (GA3). The NaCl concentrations were 0 (control), 50, 100 and 150 mM. Seed treated with GA3 showed increased water uptake and decreased electrolyte leakage as compared to that of distilled water-primed seeds even 24 h after soaking under control conditions. Seed priming with GA3 increased the final germination and the germination rate (1/t50, where t50 is the time to 50% germination) under salt stress conditions. Seed priming also alleviated the harmful effect of salt stress on cabbage in terms of fresh and dry weights. Leaf area was higher in plants raised from seeds primed with the higher GA3 concentrations as compared with those raised from seeds treated with distilled water under control conditions (without NaCl) or at 50 mM NaCl stress. The chlorophyll content increased with the NaCl concentration, especially in plants grown from seeds primed with GA3. Plants grown from GA3-primed seeds also suffered lower cellular injury both under control conditions and under NaCl stress.


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