scholarly journals Metabolomics Analysis Reveals Potential Mechanisms in Bupleurum L. (Apiaceae) Induced by Three Levels of Nitrogen Fertilization

Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2291
Author(s):  
Jialin Sun ◽  
Weinan Li ◽  
Ye Zhang ◽  
Yun Guo ◽  
Zejia Duan ◽  
...  
Keyword(s):  
Nitrogen Fertilization ◽  
Total Content ◽  
Nitrogen Level ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
High Nitrogen ◽  
Yield And Quality ◽  
High Nitrogen Level ◽  
The Impact ◽  
The Relationship

Bupleurum (Apiaceae) is widely used in traditional Chinese medicine to treat inflammatory and infectious diseases. Although roots are the only used parts in China, other countries use the whole plant. The yield and quality of Bupleurum depend mainly on fertilizers, especially nitrogen. The current study aimed to assess the relationship between the nitrogen fertilization level and the quality and metabolomic response of different parts (flowers, main shoots, lateral shoots and roots) of Bupleurum to three nitrogen fertilization levels (control group: 0 kg·ha−1; low-nitrogen group: 55 kg·ha−1; high-nitrogen group: 110 kg·ha−1). The results showed that a high nitrogen level increases Bupleurum yield and quality parameters only in aerial parts, especially flowers, but has no significant effect on roots. The HPLC method was exploited for simultaneous quantification of three saikosaponins (A, C and D), which are the main bioactive components in the plant. It was found that the total content of saikosaponins decreased with high nitrogen fertilization in roots but significantly increased in flowers. Moreover, nitrogen fertilizer promoted the content of saikosaponin A but inhibited saikosaponins C and saikosaponins D in most parts of the plant. To study the response of primary metabolites, we adopted gas chromatography–mass spectrometry (GC−MS) analysis; 84 metabolites were identified that were mostly up-regulated with a high nitrogen level in flowers but down-regulated in roots. Four differential metabolites—D-fructose, lactose, ether and glycerol—were recognized as key metabolites in Bupleurum under nitrogen fertilization. Meanwhile, The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment results explained that the impact of nitrogen fertilization on Bupleurum was attributed to the C-metabolism, N-metabolism, and lipids metabolism. This research put forward new insights into potential mechanisms and the relationship between the quality and yield of Bupleurum and nitrogen fertilization.

Quantitative analysis of mitral annulus morphology in aortic stenosis using real time 3-dimentional transesophageal echocardiography

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
W Suzuki ◽  
Y Nakano ◽  
H Ohashi ◽  
H Ando ◽  
K Waseda ◽  
...  
Keyword(s):  
Mitral Valve ◽  
Aortic Stenosis ◽  
Transesophageal Echocardiography ◽  
Real Time ◽  
Mitral Annulus ◽  
Left Ventricular Ejection ◽  
Control Group ◽  
The Impact ◽  
The Relationship ◽  
Saddle Shape

Abstract Background Normal mitral annulus morphology is known to be saddle shape. There are a few reports regarding the relationship between flattening of the mitral annular saddle shape and mitral regurgitation. However, the relationship between aortic stenosis (AS) and mitral annulus morphology is unknown. Purpose To assess the impact of AS on mitral annular saddle shape using 3-dimentional transesophageal echocardiography. Methods A total of consecutive 83 subjects including 44 patients with severe AS (AS group) and 39 patients without AS (control group), who underwent real-time 3-dimentional transesophageal echocardiography of the mitral valve, were enrolled. The 3-dimentional geometry of the mitral annulus apparatus was evaluated by the parameters analyzed using dedicated quantification software such as anteroposterior diameter (APD), commissural width (CW), annular height (AH), mitral annulus (MA) area and annular height to commissural width ratio (AHCWR) as shown in Figure. We assessed the impact of severe AS on AHCWR, which is the key parameter showing flattening of the mitral annular saddle shape. These parameters were adjusted by body surface area (BSA). Exclusion criteria included left ventricular ejection fraction <50%, the presence of aortic regurgitation, mitral valve disease, pericardial or congenital diseases, endocarditis, cardiomyopathy, prior myocardial infarction, and paroxysmal or persistent atrial fibrillation. Results Comparisons of mitral valve geometry between AS group and control group are summarized in Table. AH/BSA and AHCWR were significantly lower in AS group compared with control group. Multiple linear regression analysis revealed severe AS to be a significant and independent predictor of lowering AHCWR (β=−0.39, t=−4.04, p<0.001) (adjusted with MA area, selected by stepwise analysis). Conclusions Severe AS might contribute to flattening of the mitral annular saddle shape, lead to the mitral annular structural remodeling. Assessment of the mitral annulus morphology might help evaluating severe AS. Mitral annulus 3-dimensional geometry Funding Acknowledgement Type of funding source: None

scholarly journals From Dependence to Independence

2021 ◽  
Author(s):  
Intan Azura Mokhtar ◽  
Shaheen Majid ◽  
Schubert Foo
Keyword(s):  
Control Group ◽  
Mediated Learning ◽  
Control Group Design ◽  
Student Responses ◽  
Quasi Experimental ◽  
Information Skills ◽  
Group Interviews ◽  
Library Skills ◽  
The Impact ◽  
The Relationship

Although it is widely believed that information literacy (IL) competencies are useful in helping students perform better in their schoolwork and beyond, limited empirical evidence is available showing the relationship between IL competencies and IL education. While a lot of research has been done worldwide and most of the findings have proven that IL is a much-needed skill by students, little research has been conducted on IL teaching approaches or what is termed IL pedagogy. To date, studies on IL have mainly focused only on students’ information skills per se, on library skills or on ICT education. None of these studies has assessed the different approaches to IL education. This paper provides an overview of a research study that investigates the impact of an IL teaching approach in the form of personalised coaching, which is grounded in the pedagogy known as mediated learning, on students’ level and applicability of IL competencies. Through the application of a quasi-experimental pretest-posttest control-group design, as well as student responses in the post-experiment semi-structured group interviews, it was found that personalised coaching (or mediated learning) helps students perform better in the learning and application of IL competencies.

scholarly journals Initial CT blend sign is not associated with poor outcome in patients following stereotactic minimally invasive surgery

2021 ◽  
Author(s):  
Xu Yang ◽  
Yan Zhu ◽  
Linshan Zhang ◽  
Likun Wang ◽  
Yuanhong Mao ◽  
...  
Keyword(s):  
Minimally Invasive Surgery ◽  
Minimally Invasive ◽  
Invasive Surgery ◽  
Cardiac Complications ◽  
Control Group ◽  
Haematoma Expansion ◽  
Poor Outcomes ◽  
The Impact ◽  
The Relationship

Abstract Background: The initial computed tomography (CT) blend sign has been used as an imaging marker to predict haematoma expansion and poor outcomes in patients with a small volume intracerebral haemorrhage (ICH). However, the relationship between the blend sign and outcomes remains elusive. The present study aimed to retrospectively measure the impact of initial CT blend signs on short-term outcomes in patients with hypertensive ICH who underwent stereotactic minimally invasive surgery (sMIS). Methods: We enrolled 242 patients with spontaneous ICH. Based on the initial CT features, the patients were assigned to a blend sign group (91 patients) or a nonblend sign (control) group (151 patients). The NIHSS, GCS and mRS were used to measure the effects of sMIS. The rates of severe pulmonary infection and cardiac complications were also compared between the two groups. Results: No significant differences in NIHSS and GCS scores were observed between the two groups. The proportion of patients with good outcomes during follow-up was not different between the two groups. The rate of rehaemorrhaging increased in the blend sign group. No significant differences in severe pulmonary infections and cardiac complications were noted between the two groups. Conclusions: The initial CT blend sign was not associated with poor outcomes in patients with hypertensive ICH who underwent sMIS. ICH patients with CT blend signs should undergo sMIS if they are suitable candidates for surgery.

Prevalence of ADHD in children born by mothers consuming Frankincense during pregnancy

2021 ◽  
Author(s):  
Alimohammad Ranjbar ◽  
Elahe Kamali Ardakani ◽  
Rahele Zareshahi
Keyword(s):  
Data Gathering ◽  
Control Group ◽  
Case Group ◽  
Dsm V ◽  
History Of ◽  
The Difference ◽  
The Impact ◽  
The Relationship ◽  
The Brain ◽  
Positive Effect

Aims: In Iranian culture, due to some narratives from the prophet Mohammad about the use of frankincense during pregnancy for increasing IQ in children, some women consume frankincense during expectancy. This study's goal is to evaluate the relationship between frankincense used during pregnancy and the incidence of ADHD. Methods: In this study, the case group comprised children 4-17 years old referring to Shahid Chamran Pharmacy in Yazd from summer to winter 2018 for receiving Methylphenidate, those with whom a psychologist had identified ADHD based on DSM-V factors.  The control group included children of the same age group but without ADHD. For data gathering, a checklist was used with some questions on smoking, family history of ADHD, presence/absence of a specific disease during pregnancy, frankincense used during pregnancy, and a chemical medication consumed during pregnancy. Results: The main result demonstrated that the children whose mothers used frankincense during pregnancy were 0.67 times less likely to be affected by ADHD than those whose mothers did not use this substance. However, the difference failed to be statistically significant (P>0.05). Conclusion: Some studies report that frankincense can bear a positive effect on the development of the brain and possibly adequate formation of dendrites trees, axons and induce proper communication between them, so the impact of frankincense on the brain may be justified by its protective effect against the hyperactive child.

scholarly journals Metabonomics Study of Ginseng Glycoproteins on Improving Sleep Quality in Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Ying Wang ◽  
Difu Zhu ◽  
Yinghong Chen ◽  
Ruizhi Jiang ◽  
Hong Xu ◽  
...  
Keyword(s):  
Sleep Quality ◽  
Brain Metabolism ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
Glutamate Metabolism ◽  
Multivariate Statistical ◽  
Hormone Biosynthesis ◽  
The Relationship ◽  
Further Development

The changes of brain metabolism in mice after injection of ginseng glycoproteins (GPr) were analyzed by gas chromatography mass spectrometry- (GC/MS-) based metabolomics platform. The relationship between sedative and hypnotic effects of ginseng glycoproteins and brain metabolism was discussed. Referring to pentobarbital sodium subthreshold test, we randomly divided 20 mice into two groups: control and ginseng glycoproteins group. The mice from the control group were treated with normal saline by i.p and GPr group were treated with 60 mg/kg of GPr by i.p. The results indicated that GPr could significantly improve the sleep quality of mice. Through multivariate statistical analysis, we found that there were 23 differential metabolites in whole brain tissues between the control group and the GPr group. The pathway analysis exhibited that GPr may be involved in the regulation of the pathway including purine metabolism, nicotinate and nicotinamide metabolism, glycine, serine and threonine metabolism, arginine and proline metabolism, alanine, aspartate and glutamate metabolism, and steroid hormone biosynthesis. This work is helpful to understand the biochemical mechanism of GPr on promoting sleep and lay a foundation for further development of drugs for insomnia.

scholarly journals Тhe Impact Of Positive Acceleration (+Gz) on Antioxidant Capacity and Histopathological Alterations in Different Organs and Tissues in Rats

2017 ◽  
Vol 18 (3) ◽  
pp. 203-212
Author(s):  
Jelena Ristic ◽  
Tamara Nikolic ◽  
Jovana Jeremic ◽  
Isidora Stojic ◽  
Snezana Janicijevic-Hudomal ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Antioxidant Capacity ◽  
Total Content ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Alterations ◽  
Antioxidant Enzyme System ◽  
Biochemical Systems ◽  
Wistar Albino Rats ◽  
The Impact

Abstract Since the early 1940s, a significant amount of research has been conducted to describe the impact of the high-G acceleration on the cardiovascular system. The objective of the present study was to examine the role of the antioxidant enzyme system under biodynamic stress in the liver, heart and gastric mucosa in response to high-magnitude +Gz exposure in a rat model. Twenty adult male Wistar albino rats (10 rats per group; 9-11 weeks old, 200-250 g b.w.) were divided into the following two groups: control and G (exposed to a biodynamic stress model under positive (+7 Gz) acceleration for 40 s). The influence of acute biodynamic stress on pro-oxidative parameters in the rat liver (xanthine oxidase (XOD), catalase (CAT), peroxidase (Px), glutathione peroxidase (GSH-Px), total content of glutathione (GSH), lipid peroxidation (LPx)) and on histopathological alterations in the liver, cardiac muscle and gastric mucosa was examined. Biodynamic stress resulting from positive (+7 Gz) acceleration resulted in a highly statistically signifi cant increase of CAT GSH-Px activity compared to the control group. The LPx levels were significantly decreased, but the GSH contents and the activities of other enzymes were not significantly changed. Significant microscopic changes in the liver, heart and gastric mucosa were observed in the G group. These results clearly indicate that +Gz acceleration alters biochemical systems. These alterations in cellular processes may be mediated by influences of hypoxia or ischaemia via changes in the antioxidant capacity.

scholarly journals Relationship between Superoxide Dismutase Manganese Gene Polymorphism and Eye Tumors

2021 ◽  
Vol 9 (A) ◽  
pp. 229-232
Author(s):  
Rodiah Rahmawaty Lubis ◽  
Cut Adeya Adella ◽  
Lokot Donna Lubis
Keyword(s):  
Superoxide Dismutase ◽  
Gene Polymorphism ◽  
Gene Polymorphisms ◽  
Cancer Biology ◽  
Orbital Tumor ◽  
Control Group ◽  
Orbital Tumors ◽  
The Impact ◽  
The Relationship ◽  
Mnsod Gene

ABSTRACT   Background: Orbital tumor in Indonesia is one of the eye health problems that can cause blindness. The impact caused by orbital tumors on patients is quite large because it can result in blindness and even death due to its metastatic nature. The role that SOD plays in cancer biology is not well understood, most studies showing a more oxidative state, characterized by increased intracellular ROS, particularly superoxide. Objective: To determine the relationship between Manganese Superoxide Dismutase (SOD2) gene polymorphisms and the incidence of orbital tumors in Medan. Methods: This study is an analytic observational study with a cross-sectional data collection method using controls. Comparisons were made between the control group and the observed group to see the relationship between SOD2 polymorphisms and the risk of orbital tumor incidence in Medan. The ophthalmic examination, anterior and posterior segments, and assessment of CT orbit if deemed necessary for the orbital tumor patients. Histopathological examination was done by the Pathologist. Blood samples was taken for polymorphism examination on extracted DNA using the Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) methods. Results: About 30 patients that met the inclusion criterias. Laterality, the left eye is more likely to suffer from tumors when compared to the right eye. This study found as many as 16 patients, while malignant tumors was 14 patients. There was a relationship between the MnSOD gene polymorphism and the incidence of orbital tumors (p <0.001), there was a relationship between the MnSOD gene polymorphism and the incidence of orbital tumors in the female sex (p <0.001) Conclusion: There was a relationship between MnSOD gene polymorphisms and the incidence of orbital tumors (p <0.001)

scholarly journals THE ROLE OF INFLAMMATION AND COAGULATION CASCADE IN THE PATHOGENESIS OF ATRIAL FIBRILLATION

ASJ. ◽  
2020 ◽  
Vol 3 (41) ◽  
pp. 8-10
Author(s):  
L. Hazarapetyan ◽  
S. Grigoryan ◽  
A. Sarksyan
Keyword(s):  
Atrial Fibrillation ◽  
Coagulation Cascade ◽  
Control Group ◽  
Atrial Remodeling ◽  
Inflammation Markers ◽  
Reactive Protein ◽  
Gender And Age ◽  
The Impact ◽  
The Relationship

Introduction: Atrial fibrillation (AF) is associated with prothrombotic or hypercoagulable states, various inflammation markers such as interleukin-6 (IL-6) and hsC-reactive protein (hsCRP) have also been associated with AF. The aim of this study is to investigate the relationship between inflammation markers and the prothrombotic state in the setting of AF and the impact on outcome in patients with AF. Methods: We observed 141 patients with non-valvular AF. As a control group patients similar in gender and age without AF were examined. Clinical, instrumental and laboratory tests were performed on the observed patients. The markers of the coagulation cascade (TF and F) and of inflammatory markers (hsCRP and IL-6) were studied additionally by ELISA on the analyzer "Stat Fax 303 Plus". Studies were conducted using SPSS 13.0 and EXCEL-2013. Results: The obtained results showed that compared to the control group, AF patients had significantly higher levels of IL-6 (p = 0.043), hsCRP (p = 0.002), TF (p = 0.026), and F (p = 0.025). Moreover, levels of hsCRP were higher among AF patients at "high" risk of stroke by CHA2DS2-VASc Score (p = 0.003). Besides, the levels of hsCRP and IL-6 were markedly elevated in patients with dilated left atrium (p = 0.001), poorly functioning left atrial appendage (p = 0.023) and longer duration of AF (p = 0.002). Conclusion: We have demonstrated that the increased plasma levels of IL-6 and hsCRP are related to indices of the coagulation cascade and contribute to structural atrial remodeling in patients with AF.

scholarly journals Impact of Irrigation and Fertigation on the Yield and Quality of Sugar Beet (Beta vulgaris L.) in a Moderate Climate

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 166 ◽  
Author(s):  
Jacek Żarski ◽  
Renata Kuśmierek-Tomaszewska ◽  
Stanisław Dudek
Keyword(s):  
Sugar Beet ◽  
Nitrogen Fertilization ◽  
Sugar Content ◽  
Sprinkler Irrigation ◽  
Irrigation Amount ◽  
Beet Root ◽  
Yield And Quality ◽  
The Impact ◽  
Moderate Climate

In Poland, under conditions of the moderate climate and transition between maritime and continental climates, the average rainfall totals of the growing season are in the range of 350–400 mm; however, they are distinguished by great temporal and spatial variability. Climatological studies demonstrate that the drought frequency is approximately 30%. Therefore, under such conditions, irrigation has a supplementary and intervention nature and is applied only when dry periods occur. The aim of this study was to determine the impact of sprinkler irrigation and increased nitrogen fertilization on the yield and quality of sugar beet roots and yield of sugar. The average increase of the yield under irrigation was 18.1 t·ha−1 which constituted a 22.8% increase in the yield. Furthermore, there was a marked tendency of a higher sugar content in the roots of irrigated plants. The absolute, relative, and unit average sugar beet root yield increases obtained under the influence of sprinkler irrigation and the lack of a significant diversity in the sugar content in roots confirm that irrigation contributed to an appropriate pace of plant growth and development. The increased rate of nitrogen fertilization (N2) of 160 kg N·ha−1 plus an additional 40 kg N·ha−1 resulted in the significantly greater root yields compared to the control (N1) (160 kg N·ha−1), i.e., an average of 7.6 t·ha−1 (9%). Based on the crop-water production function, the maximum root yields were obtained for the N1 rate at a total precipitation and irrigation amount of 382 mm, compared with 367 mm for the N2 rate.

Growth, yield, and fruit quality in a factorial field experiment with citrus in relation to changes in phosphorus nutrition

1959 ◽  
Vol 10 (1) ◽  
pp. 41 ◽  
Author(s):  
D Bouma
Keyword(s):  
Field Experiment ◽  
Fruit Quality ◽  
Nitrogen Fertilization ◽  
Growth Yield ◽  
Nitrogen Level ◽  
Depressing Effect ◽  
Nitrogen And Phosphorus ◽  
Nitrogen Levels ◽  
Yield And Quality

Changes in growth, yield, and fruit quality of citrus in a factorial field experiment are presented. Nitrogen fertilization in the sod treatment had only limited effects on yield. After the application of superphosphate, yields at the higher nitrogen levels increased markedly. The initially superior yields from the bare surface treatment decreased gradually, and became at the highest nitrogen level even lower than in the tilled treatments. After application of superphosphate, yields improved in the plots receiving ample nitrogen and became even higher than in the tilled treatments. Yields in the tilled treatments remained good, and were not influenced by nitrogen fertilization. Fruit quality in the no-tillage treatments improved considerably after the application of superphosphate. Increasing nitrogen supply, however, had a depressing effect on fruit quality in all cultural treatment's, but within each nitrogen level the fruit was of similar quality. Nitrogen and phosphorus contents of leaves sampled in 1954 and 1957 are compared, and the changes in yield and quality that occurred during that period are discussed in relation to the modified phosphorus supply in the experiment.

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