scholarly journals Weaned Sows with Small Ovarian Follicles Respond Poorly to the GnRH Agonist Buserelin

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1979
Author(s):  
Tania P. Lopes ◽  
Lorena Padilla ◽  
Alfonso Bolarin ◽  
Heriberto Rodriguez-Martinez ◽  
Jordi Roca
Keyword(s):  
Gnrh Agonist ◽  
Treatment Time ◽  
Time Window ◽  
Ovarian Follicle ◽  
Ovarian Follicles ◽  
Expected Time ◽  
Buserelin Acetate ◽  
Follicular Size ◽  
Follicle Size ◽  
And Control

The GnRH agonist buserelin (GnRH), used to synchronize ovulation in weaned sows, attains only 70–80% effectivity, owing to several reasons of ovarian origin. This study evaluated in particular whether mean ovarian follicle size at treatment and the season of weaning are among those influencing GnRH responsiveness. The experiment was carried out in a temperate-region farm with 352 sows of 1–6 parities weaned either in winter–spring (WS, 174 sows) or in summer–autumn (SA, 178 sows). The sows were randomized into two groups: GnRH (10 µg of buserelin acetate at 86 h after weaning, 172 sows) and control (180 sows). The ovaries were transrectally scanned from weaning to ovulation and the sows clustered according to their mean follicular size at treatment time: small (<0.5 cm in diameter), medium (0.5 to 0.64 cm) and large (0.65 to 1.09 cm). In total, 88.33% of the GnRH-treated sows ovulated, with 82% of them within the expected time window (120–132 h after weaning). In contrast, 95.45% of the unresponsive sows had small follicles at the time of treatment and were mostly weaned in SA (20.45%) than in WS (4.76%). In conclusion, the conspicuous presence of sows having small ovarian follicles at treatment time compromises the efficiency of the GnRH agonist buserelin to synchronize ovulation in weaned sows, which occurs more frequently in summer–autumn weaning.

scholarly journals Ovarian Follicle Growth during Lactation Determines the Reproductive Performance of Weaned Sows

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1012 ◽  
Author(s):  
Tania P. Lopes ◽  
Lorena Padilla ◽  
Alfonso Bolarin ◽  
Heriberto Rodriguez-Martinez ◽  
Jordi Roca
Keyword(s):  
Reproductive Performance ◽  
Post Partum ◽  
Ovarian Follicle ◽  
Transrectal Ultrasound ◽  
Great Variability ◽  
Early Lactation ◽  
Follicle Growth ◽  
Follicle Diameter ◽  
Follicular Size ◽  
Follicle Size

Factors causing variability in ovarian follicle size among weaned sows are not well known. This field study aimed to disclose influencing factors and evaluate if the differences at weaning were established during lactation. Ovaries were scanned using transrectal ultrasound. The first experiment was conducted over a year with 191 randomly chosen sows that were hierarchically grouped (p < 0.001) according to ovarian follicle diameter reached at weaning: Small (0.20–0.30 cm; n = 37), medium (0.31–0.39 cm; n = 75), and large (0.40-1.00 cm; n = 69). Sows with small follicles showed a higher incidence of post-weaning anestrus (p < 0.01), longer wean-to-estrus/ovulation intervals (p < 0.01) and farrowing smaller litters (p < 0.05). Ovaries with small follicles were more common among sows weaned in summer–autumn than in winter–spring (p < 0.01) and among sows of lower parity (1–3) (p < 0.05). In the second experiment, with 40 sows randomly chosen at farrowing, the ovaries were scanned at 7, 14, and 21 d post-partum. Sows showed great variability in ovarian follicular size during lactation with a consistent relationship between the three measurement times (r = 0.84, p < 0.01). Follicle size was smaller in sows nursing in summer–autumn than in winter–spring (p < 0.05). In conclusion, early lactation dictates the great variability in ovarian follicular diameter at weaning shown by sows. Sows with smaller follicles at weaning had longer intervals for estrus and ovulation and smaller litters at farrowing and they were in greater numbers among sows weaned during the summer and fall and among those with fewer previous farrowing.

Oestrus synchronisation and fixed time artificial insemination in beef heifers

2010 ◽  
Vol 50 (7) ◽  
pp. 670
Author(s):  
Daniel Cavestany ◽  
Daniela Crespi ◽  
Alvaro Fernandez
Keyword(s):  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Conception Rate ◽  
Control Group ◽  
Beef Heifers ◽  
Follicular Size ◽  
Follicle Size ◽  
And Control

To compare two oestrus synchronisation protocols in beef heifers presynchronised with two prostaglandin F2α (PG) treatments, 36 h after the second PG treatment one group (hCG-OB group; n = 112) received 250 IU of hCG and 0.5 mg of oestradiol benzoate (OB) followed by fixed time artificial insemination (FTAI) 30 h later. Additional oestrus detection (OD) and artificial insemination (AI) was done in this group for 3 days after FTAI; heifers rebred during this period were considered open to the FTAI. The Control group (n = 112) received only OD and AI for 6 days after the second PG treatment. Follicular size was measured at the time of AI in a subsample of heifers (59 in the hCG-OB group and 34 in the Control group). Results were evaluated separately either considering heifers pregnant at FTAI or those pregnant at FTAI + 3-day OD + AI. The FTAI conception rate was 45.5 vs 76.5% for the Control group (P < 0.0001) and pregnancy rates were 45.5 and 67.0% (P = 0.0014); FTAI + OD + AI conception rates were 63.4 and 76.2% (P = 0.0404) and pregnancy rates 63.4 and 62.8% (P = 0.5719) (hCG-OB and Control groups, respectively). There was a significant correlation (r = 0.713, P < 0.01) between follicle size at FTAI and pregnancy in the hCG-OB group, but not in the Control group (r = 0.3465, P > 0.1). In conclusion, acceptable synchronised conception rate and pregnancy rate were obtained by using this protocol, and the results were improved by complementing it with OD + AI for 3 days after FTAI.

scholarly journals Relaxin protein and gene expression in ovarian follicles of immature pigs

1998 ◽  
Vol 21 (2) ◽  
pp. 179-187 ◽  
Author(s):  
KM Ohleth ◽  
Q Zhang ◽  
CA Bagnell
Keyword(s):  
Gene Expression ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Ovarian Follicle ◽  
Follicular Development ◽  
Ovarian Follicles ◽  
In Vitro Growth ◽  
Follicle Size ◽  
Theca Interna

Relaxin production by the ovarian follicle of gonadotropin-primed, prepubertal gilts is well documented. As far as we are aware, a source of relaxin in pig follicles, independent of gonadotropins, has not yet been reported. Therefore, the objective of this study was to determine whether relaxin is produced in porcine follicles in the absence of exogenous or cyclic gonadotropins. In immature pigs, immunoreactive relaxin was detected in fluids from small (1-3 mm), medium (4-5 mm) and large (>6 mm) follicles and localized to the theca interna of large follicles. Relaxin levels in follicular fluid significantly increased with follicle size (P<0.05). Relaxin mRNA was detected in whole small- and medium-sized follicles. In large follicles, the relaxin gene was expressed in thecal layers, but not granulosa cells. The abundance of relaxin transcript did not change with follicle size. In summary, relaxin protein and mRNA were detected in porcine follicles from immature animals, indicating that relaxin is produced in the porcine follicle in the absence of exogenous or cyclic gonadotropins. Relaxin's in vitro growth effects on porcine granulosa and theca cells support this follicular relaxin as a growth modulator during porcine follicular development.

An evaluation model of 2019-nCoV prevention effectiveness based on immigration data in China (Preprint)

2020 ◽  
Author(s):  
Qiangsheng Huang
Keyword(s):  
Prevention And Control ◽  
Time Window ◽  
Evaluation Model ◽  
Control Measures ◽  
Related Data ◽  
The Face ◽  
Prevention And Control Measures ◽  
And Control ◽  
Best Fit

BACKGROUND As of the end of February 2020, 2019-nCoV is currently well controlled in China. However, the virus is now spreading globally. OBJECTIVE This study aimed to evaluate the effectiveness of outbreak prevention and control measures in a region. METHODS A model is built for find the best fit for two sets of data (the number of daily new diagnosed, and the risk value of incoming immigration population). The parameters (offset and time window) in the model can be used as the evaluation of effectiveness of outbreak prevention and control. RESULTS Through study, it is found that the parameter offset and time window in the model can accurately reflect the prevention effectiveness. Some related data and public news confirm this result. And this method has advantages over the method using R0 in two aspects. CONCLUSIONS If the epidemic situation is well controlled, the virus is not terrible. Now the daily new diagnosed patients in most regions of China is quickly reduced to zero or close to zero. Chinese can do a good job in the face of huge epidemic pressure. Therefore, if other countries can do well in prevention and control, the epidemic in those places can also pass quickly.

scholarly journals Feasibility of Secondary Follicle Isolation, Culture and Achievement of In-Vitro Oocyte Maturation from Superovulated Ovaries: An Experimental Proof-of-Concept Study Using Mice

2021 ◽  
Vol 10 (13) ◽  
pp. 2757
Author(s):  
Xia Hao ◽  
Amandine Anastácio ◽  
Kenny A. Rodriguez-Wallberg
Keyword(s):  
Oocyte Maturation ◽  
In Vitro Maturation ◽  
Follicular Development ◽  
Clinical Situation ◽  
Experimental Proof ◽  
Secondary Follicle ◽  
17Β Estradiol ◽  
Follicle Size ◽  
And Control

Fertility preservation through ovarian stimulation, aiming at cryopreserving mature oocytes or embryos, is sometimes unsuccessful. This clinical situation deserves novel approaches to overcome infertility following cancer treatment in patients facing highly gonadotoxic treatment. In this controlled experimental study, we investigated the feasibility of in-vitro culturing secondary follicles isolated from superovulated ovaries of mice recently treated with gonadotropins. The follicle yields of superovulated ovaries were 45.9% less than in unstimulated controls. Follicles from superovulated ovaries showed faster growth pace during the initial 7 days of culture and secreted more 17β-estradiol by the end of culture vs controls. Parameters reflecting the outcome of follicular development and oocyte maturation competence in vitro were similar between superovulated and control groups, with a similar follicle size at the end of culture and around 70% survival. Nearly half of cultured follicles met the criteria for in-vitro maturation in both groups and approximately 60% of those achieved a mature MII oocyte, similarly in both groups. Over 60% of obtained MII oocytes displayed normal-looking spindle and chromosome configurations, without significant differences between the groups. Using a validated follicle culture system, we demonstrated the feasibility of secondary follicle isolation, in-vitro culture and oocyte maturation with normal spindle and chromosome configurations obtained from superovulated mice ovaries.

scholarly journals Signalling pathways and mechanistic cues highlighted by transcriptomic analysis of primordial, primary, and secondary ovarian follicles in domestic cat

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shauna Kehoe ◽  
Katarina Jewgenow ◽  
Paul R. Johnston ◽  
Susan Mbedi ◽  
Beate C. Braun
Keyword(s):  
Gene Expression ◽  
Transforming Growth Factor ◽  
Ovarian Follicle ◽  
Mammalian Species ◽  
Expression Patterns ◽  
Ovarian Follicles ◽  
Domestic Cat ◽  
Transcriptional Analysis ◽  
Ovarian Folliculogenesis

AbstractIn vitro growth (IVG) of dormant primordial ovarian follicles aims to produce mature competent oocytes for assisted reproduction. Success is dependent on optimal in vitro conditions complemented with an understanding of oocyte and ovarian follicle development in vivo. Complete IVG has not been achieved in any other mammalian species besides mice. Furthermore, ovarian folliculogenesis remains sparsely understood overall. Here, gene expression patterns were characterised by RNA-sequencing in primordial (PrF), primary (PF), and secondary (SF) ovarian follicles from Felis catus (domestic cat) ovaries. Two major transitions were investigated: PrF-PF and PF-SF. Transcriptional analysis revealed a higher proportion in gene expression changes during the PrF-PF transition. Key influencing factors during this transition included the interaction between the extracellular matrix (ECM) and matrix metalloproteinase (MMPs) along with nuclear components such as, histone HIST1H1T (H1.6). Conserved signalling factors and expression patterns previously described during mammalian ovarian folliculogenesis were observed. Species-specific features during domestic cat ovarian folliculogenesis were also found. The signalling pathway terms “PI3K-Akt”, “transforming growth factor-β receptor”, “ErbB”, and “HIF-1” from the functional annotation analysis were studied. Some results highlighted mechanistic cues potentially involved in PrF development in the domestic cat. Overall, this study provides an insight into regulatory factors and pathways during preantral ovarian folliculogenesis in domestic cat.

Development of the first urinary reproductive hormone ranges referenced to independently determined ovulation day

2015 ◽  
Vol 53 (7) ◽  
Author(s):  
Sarah Johnson ◽  
Sarah Weddell ◽  
Sonya Godbert ◽  
Guenter Freundl ◽  
Judith Roos ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Hormone Level ◽  
Ovarian Follicle ◽  
Reproductive Hormone ◽  
Fertility Status ◽  
Daily Urine ◽  
Follicle Size ◽  
Status Information ◽  
Progesterone Metabolite ◽  
Level Analysis

AbstractUrinary hormone level analysis provides valuable fertility status information; however, previous studies have not referenced levels to the ovulation day, or have used outdated methods. This study aimed to produce reproductive hormone ranges referenced to ovulation day determined by ultrasound.Women aged 18–40 years (no reported infertility) collected daily urine samples for one complete menstrual cycle. Urinary luteinising hormone (LH), estrone-3-glucuronide (E3G, an estradiol metabolite), follicle stimulating hormone (FSH) and pregnanediol-3-glucuronide (P3G, a progesterone metabolite) were measured using previously validated assays. Volunteers underwent trans-vaginal ultrasound every 2 days until the dominant ovarian follicle size reached 16 mm, when daily scans were performed until ovulation was observed. Data were analysed to create hormone ranges referenced to the day of objective ovulation as determined by ultrasound.In 40 volunteers, mean age 28.9 years, urinary LH surge always preceded ovulation with a mean of 0.81 days; thus LH is an excellent assay-independent predictor of ovulation. The timing of peak LH was assay-dependent and could be post-ovulatory; therefore should no longer be used to predict/determine ovulation. Urinary P3G rose from baseline after ovulation in all volunteers, peaking a median of 7.5 days following ovulation. Median urinary peak E3G and FSH levels occurred 0.5 days prior to ovulation. A persistent rise in urinary E3G was observed from approximately 3 days pre- until 5 days post-ovulation.This study provides reproductive hormone ranges referenced to the actual day of ovulation as determined by ultrasound, to facilitate examination of menstrual cycle endocrinology.

Review of clinical trials of neuroprotection in acute spinal cord injury

1999 ◽  
Vol 6 (1) ◽  
pp. E10 ◽  
Author(s):  
Charles H. Tator ◽  
Michael G. Fehlings
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Clinical Trials ◽  
Treatment Time ◽  
Time Window ◽  
Current Knowledge ◽  
Acute Spinal Cord Injury ◽  
Randomized Controlled Studies ◽  
Cord Injury ◽  
Therapeutic Time Window

In this paper the authors review the clinical trials of neuroprotection that have been performed for the treatment of acute spinal cord injury (SCI). The biological rationale for the selection of each treatment modality is discussed with reference to current knowledge of the principles in the management of acute SCI as well as the primary and secondary injury mechanisms identified by experimental and clinical studies of the pathophysiology of acute SCI. The trials are evaluated with regard to the availability and use of accurate clinical outcome measures, and the methodologies of the trials are critically evaluated with an emphasis on prospective randomized controlled studies. A detailed description and critical analysis are provided of the results of the 10 clinical trials conducted to date in which a randomized prospective controlled design has been used. The issue of the therapeutic time window in acute SCI is discussed. To date, methylprednisolone is the only effective neuroprotective agent that has been established for use in human SCI, and the only therapeutic time window established in human SCI is a maximum trauma-to-treatment time of 8 hours.

scholarly journals Superconductor qubits hamiltonian approximations effect on quantum state evolution and control

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Javad Sharifi
Keyword(s):  
Quantum State ◽  
Charge Qubit ◽  
Gate Operation ◽  
Goal State ◽  
Expected Time ◽  
State Evolution ◽  
Angle Of Deviation ◽  
Hadamard Gate ◽  
Phase Qubit ◽  
And Control

AbstractMicrowave IQ-mixer controllers are designed for the three approximated Hamiltonians of charge, phase and flux qubits and the controllers are exerted both on approximate and precise quantum system models. The controlled qubits are for the implementation of the two quantum-gates with these three fundamental types of qubits, Quantum NOT-gate and Hadamard-gate. In the charge-qubit, for implementation of both gates, in the approximated and precise model, we observed different controlled trajectories. But fortunately, applying the controller designed for the approximated system over the precise system leads to the passing of the quantum state from the desired state sooner that the expected time. Phase-qubit and flux qubit have similar behaviour under the control system action. In both of them, the implementation of NOT-gate operation led to same trajectories which arrive at final goal state at different times. But in both of those two qubits for implementation of Hadamard-gate, desired trajectory and precise trajectory have some angle of deviation, then by exerting the approximated design controller to precise system, it caused the quantum state to approach the goal state for Hadamard gate implementation, and since the quantum state does not completely reach the goal state, we can not obtain very high gate fidelity.

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