scholarly journals Changes in the Relationship between Ionized and Total Calcium in Clinically Healthy Dairy Cows in the Period around Calving

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1036
Author(s):  
Dorothee Ott ◽  
Katharina Schrapers ◽  
Jörg Aschenbach
Keyword(s):  
Dairy Cows ◽  
Blood Gases ◽  
Total Calcium ◽  
Multivariable Model ◽  
Hydroxybutyric Acid ◽  
Blood Constituents ◽  
Esterified Fatty Acids ◽  
Routine Measurement ◽  
Serum Ionized Calcium ◽  
The Relationship

We aimed to establish a model for prediction of iCa from tCa, using multivariable regressions with diverse blood constituents. Blood was taken from 14 cows at days −2, 0, 2, 4, 7, and 14 relative to parturition. Cows were clinically healthy, and no hypocalcaemia prophylaxis and treatment were applied. Total calcium and further parameters were determined from frozen serum. Ionized calcium, blood gases, and electrolytes were determined from heparin-stabilized blood samples. Linear regression between iCa and tCa was estimated. Precision improved only slightly using a multivariable model. Best precision was achieved when estimating the iCa:tCa ratio from other blood constituents. To identify the reason behind the poorly predictive value of tCa for iCa, the relative changes of iCa and tCa around calving were calibrated to the respective values of day −2 (=100%) for each cow. An increase in the iCa:tCa ratio was observed from 0.43 at day −2 to 0.48 at day 0, followed by a gradual decrease towards 0.43 at day 7. We conclude that routine measurement of iCa should be implemented in the diagnosis of hypocalcaemia. An optimized estimate of iCa from tCa with non-esterified fatty acids (NEFA), beta-hydroxybutyric acid, cholesterol, and phosphorous as co-predictors is still poorly satisfying.

Relationship between serum lactate and ionized calcium in open-heart surgery.

1986 ◽  
Vol 32 (10) ◽  
pp. 1849-1853 ◽  
Author(s):  
J Toffaletti ◽  
R H Christenson ◽  
S Mullins ◽  
R E Harris
Keyword(s):  
Linear Regression ◽  
Heart Surgery ◽  
Open Heart Surgery ◽  
Blood Gases ◽  
Serum Lactate ◽  
Ionized Calcium ◽  
Open Heart ◽  
Total Calcium ◽  
Before And After ◽  
The Relationship

Abstract We studied 16 patients undergoing open-heart surgery and heart-lung bypass, to examine the relationship between ionized calcium and lactate. Blood was sampled at successive stages of the operation for measurement of ionized and total calcium, lactate, blood gases, pH, hematocrit, and other constituents. We found that correlations between ionized calcium and lactate were positive and statistically significant (p less than 0.05), both among and within patients. The linear regression of ionized calcium on lactate remained highly significant (p less than 0.0001) after adjustment for variability among patients and across operative stages as well as after correction for pH and hemodilution. The significant regressions between calcium and lactate, both before and after administration of calcium, indicate a relationship for calcium and lactate in patients undergoing open-heart surgery.

scholarly journals Ketone bodies in blood of dairy cows: Prevalence and monitoring of subclinical ketosis

2014 ◽  
Vol 83 (4) ◽  
pp. 411-416 ◽  
Author(s):  
Marek Krempaský ◽  
Iveta Maskaľová ◽  
Lukáš Bujňák ◽  
Vladimír Vajda
Keyword(s):  
Fatty Acid ◽  
Dairy Cows ◽  
Ketone Bodies ◽  
Photometric Method ◽  
Hydroxybutyric Acid ◽  
Subclinical Ketosis ◽  
Cattle Herds ◽  
Lactation Phase ◽  
The Relationship ◽  
Photometrical Method

The aim of this study was to investigate the relationship between concentration of non-esterified fatty acid and ketone bodies in blood of dairy cows, and to evaluate these concentrations for the detection of prevalence of subclinical ketosis. The second aim was to compare the concentration of β-hydroxybutyric acid determined by an electronic handheld meter Precision Xtra® with serum concentration of β-hydroxybutyric acid analysed in laboratory with izotachometric and photometric method, respectively. Blood samples were collected from jugular vein 4–6 h after morning feeding in three groups of Holstein cows (n = 909) according to the lactation phase from 51 different herds with similar husbandry characteristics. High lipomobilization (non-esterified fatty acid ≥ 0.35 mmol·l-1, mean concentration 0.34 ± 0.15 mmol·l-1) was detected in 30.3% of antepartum cows, while increased concentrations of β-hydroxybutyric acid (≥ 1.0 mmol·l-1, prevalence of subclinical ketosis) were detected in 18.5% and 14.1% of the early lactation and mid lactation cows, respectively. The correlation coefficient (r = 0.84, P < 0.001; r = 0.93, P < 0.001) was found between measurements of whole blood β-hydroxybutyric acid of 60 and 38 dairy cows determined with the Precision Xtra® test and plasma or serum β-hydroxybutyric acid concentration determined by isotachophoresis and photometrical method, respectively. Our results show that the monitoring of changes in the blood concentration of β-hydroxybutyric acid in high-yielding cows in the early postpartum period by the electronic handheld meter Precision Xtra® may be effective in reducing the incidence of ketosis and health problems associated with ketosis in dairy cattle herds.

The relationship between post-parturient fatty liver and blood composition in dairy cows

1983 ◽  
Vol 101 (2) ◽  
pp. 473-480 ◽  
Author(s):  
I. M. Reid ◽  
G. J. Rowlands ◽  
A. M. Dew ◽  
R. A. Collins ◽  
C. J. Roberts ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Liver ◽  
Dairy Cows ◽  
Aspartate Aminotransferase ◽  
Blood Composition ◽  
Blood Concentrations ◽  
Blood Samples ◽  
Esterified Fatty Acids ◽  
Aspartate Amino Transferase ◽  
The Relationship

SUMMARYThe blood composition of 45 cows with more than 20% fat in the liver (moderate fatty liver) was compared with that of 83 cows with less than 20% fat in the liver (mild fatty liver). The cows were sampled at 1 week after calving and were from three Friesian and one Guernsey herds. Blood concentrations of non-esterified fatty acids, bilirubin and aspartate amino-transferase were significantly elevated in cows with more than 20% in the liver whereas concentrations of glucose, cholesterol, albumin and magnesium were all significantly reduced. Blood samples from cows in two of the herds were analysed for ammonia, insulin and D(-)3-hydroxybutyrate. Cows with moderate fatty liver in these two herds had significantly lower insulin concentrations and higher D(-)3-hydroxybutyrate concentrations than those with mild fatty liver. Using an equation based on blood concentrations of non-esterified fatty acids, glucose and aspartate aminotransferase it was possible to assign correctly three out of four cows to either the mild or moderate fatty liver groups.

Relation between intravascular electrolyte level and course of parturition in dairy cows

2013 ◽  
Vol 41 (05) ◽  
pp. 289-296 ◽  
Author(s):  
K. Seyrek-Intas ◽  
K. Failing ◽  
G. Yilmazbas Mecitoglu ◽  
H. Bostedt ◽  
D. Seyrek-Intas
Keyword(s):  
Dairy Cows ◽  
Inorganic Phosphate ◽  
Post Partum ◽  
Total Calcium ◽  
Normal Delivery ◽  
P Values ◽  
Normal Birth ◽  
Factors Influencing ◽  
Electrolyte Homeostasis ◽  
Convalescence Phase

Summary Objective: To determine the intravascular electrolyte status in dairy cows with respect to age and different courses of parturition to clarify etiological factors influencing peri- or intrapartal imbalances of electrolyte homeostasis. Material and methods: A total of 64 cows at birth were evaluated (primiparous n = 34, pluriparous n = 30). Thirty-three cows showed normal delivery, while 31 cows had a complicated birth. Blood samples were collected intra partum (i. p.) and 2, 4, 6, 8, 12, 16, 24 h post partum (p. p.) as well as [2, 3, 4, 5, 7, 10 days p. p. and levels of total calcium (Catotal), ionized calcium (Caion), inorganic phosphate (Pa), Mg, Na, K, Cl were determined. Results: The results revealed that electrolytes show great fluctuation during and immediately p. p. in relation to age. Already during parturition pluriparous cows had a lower Catotal and Pa concentration compared to primiparous animals. Cows with dystocia exhibited a more intensive and longer lasting decrease of Ca compared to cows with normal birth. In relation to age and severity of birth Pa concentration showed a differing but basically typical course for this electrolyte. Mg, Na, K and Cl concentrations were higher during and immediately after birth compared to p. p. values. Until day 10 p. p. these electrolyte concentrations declined more in older cows with dystocia compared to younger animals. However, the influence of dystocia on concentration of these electrolytes was milder in contrast to Ca and Pa. Conclusions: In summary, primarily older cows are predisposed to imbalances of electrolyte homeostasis intra partum and at the beginning of the lactation. These changes are potentiated in case of complications during parturition. Intravascular Catotal, Caion as well as Pa are most severely affected. Clinical relevance: These results may constitute the basis for a comprehensive metaphylaxis during the peripartal period, especially in cows after dystocia, to positively influence the early convalescence phase.

The Relationship of Certain Blood Constituents to Rate and Economy of Gain in Beef Cattle

1959 ◽  
Vol 18 (1) ◽  
pp. 314-322 ◽  
Author(s):  
R. L. Arthaud ◽  
A. B. Schultze ◽  
R. M. Koch ◽  
V. H. Arthaud
Keyword(s):  
Beef Cattle ◽  
Blood Constituents ◽  
Relationship Of ◽  
The Relationship

scholarly journals PSII-17 Program Chair Poster Pick: Effect of ruminal protozoa on methane emissions in ruminants – a meta-analysis

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 397-398
Author(s):  
Xiaoxia Dai ◽  
Kenneth Kalscheur ◽  
Pekka Huhtanen ◽  
Antonio Faciola
Keyword(s):  
Dairy Cows ◽  
Meta Analysis ◽  
Random Effect ◽  
Negative Relationship ◽  
Small Ruminants ◽  
Methane Emissions ◽  
Mitigation Strategies ◽  
Positive Relationships ◽  
Ruminal Fermentation ◽  
The Relationship

Abstract The effects of ruminal protozoa (RP) concentration on methane emissions from ruminants were evaluated in a meta-analysis using 67 publications reporting data from 85 in vivo experiments. Experiments included in the database reported methane emissions (g/kg DMI) and RP (log10 cells/mL) from the same group of animals. Quantitative data including diet chemical composition, ruminal fermentation, total tract digestibility, and milk production; and qualitative information including methane mitigation strategies, animal type, and methane measurement methods were also collected. The studies were conducted in dairy cows (51%), beef steers (32%) and small ruminants (32%). 70% of the studies reported a reduction in methane emissions. Supplemental lipids reduced methane emissions 95% of the time. The relationship between methane emissions and RP concentration was evaluated as a random coefficient model with the experiment as a random effect and weighted by the inverse pooled SEM squared, including the possibility of covariance between the slope and the intercept. A quadratic effect of RP concentration on methane emissions was detected: CH4= -28.8 + 12.2 × RP-0.64 × RP2. To detect potential interfering factors in the relationship, the influence of several qualitative and quantitative factors were separately tested. Acetate, butyrate, and isobutyrate molar proportions had positive relationships with methane emissions and influenced the relationship between RP concentration and methane emissions, where the presence of ruminal fermentation variables reduced the effects of RP concentration in methane emissions. Total tract digestibility of DM, OM, and CP had negative relationships while NDF digestibility had a positive relationship with methane emissions; however, they only changed the magnitude of intercept and slope of RP and RP2 for the relationship. For dairy cows, milk fat and protein concentrations had positive relationships and milk yield had a negative relationship with methane emissions and changed the magnitude of intercept and slope of RP and RP2 for the relationship.

scholarly journals Prediction of Reproductive Success in Multiparous First Service Dairy Cows by Parameters from In-Line Sensors

Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 334
Author(s):  
Ramūnas Antanaitis ◽  
Vida Juozaitienė ◽  
Dovilė Malašauskienė ◽  
Mindaugas Televičius ◽  
Mingaudas Urbutis ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Blood Parameters ◽  
Negative Energy ◽  
Hydroxybutyric Acid ◽  
Pregnancy Status ◽  
Group A ◽  
Biochemical Indices ◽  
Relationship Of ◽  
The Relationship

The aim of the current study was to evaluate the relationship of different parameters from an automatic milking system (AMS) with the pregnancy status of multiparous cows at first service and to assess the accuracy of such a follow-up with regard to blood parameters. Before the insemination of cows, blood samples for measuring biochemical indices were taken from the coccygeal vessels and the concentrations of blood serum albumin (ALB), cortisol, non-esterified fatty acids (NEFA) and the activities of aspartate aminotransferase (AST) and gamma glutamyltransferase (GGT) were determined. From oestrus day to seven days after oestrus, the following parameters were registered: milk yield (MY), electric milk conductivity, lactate dehydrogenase (LDH) and β-hydroxybutyric acid (BHB). The pregnancy status was evaluated using ultrasound “Easy scan” 30–35 days after insemination. Cows were grouped by reproductive status: PG− (non-pregnant; n = 48) and PG+ (pregnant; n = 44). The BHB level in PG− cows was 1.2 times higher (p < 0.005). The electrical conductivity of milk was statistically significantly higher in all quarters of PG− cows (1.07 times) than of PG+ cows (p < 0.05). The arithmetic mean of blood GGT was 1.61 times higher in PG− cows and the NEFA value 1.23 times higher (p < 0.05) compared with the PG+ group. The liver function was affected, the average ALB of PG− cows was 1.19 times lower (p < 0.05) and the AST activity was 1.16 times lower (p < 0.05) compared with PG+ cows. The non-pregnant group had a negative energy balance demonstrated by high in-line milk BHB and high blood NEFA concentrations. We found a greater number of cows with cortisol >0.0.75 mg/dL in the non-pregnant group. A higher milk electrical conductivity in the non-pregnant cows pointed towards a greater risk of mastitis while higher GGT activities together with lower albumin concentrations indicated that the cows were more affected by oxidative stress.

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