scholarly journals Small RNA-Seq Analysis Reveals miRNA Expression of Short Distance Transportation Stress in Beef Cattle Blood

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2850
Author(s):  
Mingli Wu ◽  
Xiaoqin Tang ◽  
Sayed Haidar Abbas Raza ◽  
Haidong Zhao ◽  
Qi Li ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Short Distance ◽  
Economic Losses ◽  
Molecular Characteristics ◽  
Hub Genes ◽  
Long Distance ◽  
Potential Health ◽  
Before And After ◽  
Cattle Blood ◽  
Transportation Stress

Transportation is a crucial phase in the beef cattle industry, and the annual losses caused by beef cattle transport stress are substantial. Because of its huge economic losses, such as lower growth rate and even death, long-distance transportation stress has attracted more attention from beef production practitioners because of its huge economic losses. Compared with the long-distance transportation stress, the short-distance transportation stress was ignored for the reason of no obvious symptoms in cattle. Our previous study showed that the disorder of B cell function could be a potential health risk after short-distance transportation. However, the transcriptome details of the changes in the cattle blood after short-distance transportation and the molecular mechanisms for the regulation of the developmental process are not clearly known. In this study, a total of 10 Qinchuan cattle were used to compare the molecular characteristics of blood before and after short-distance transportation. The miRNA-seq showed that 114 differentially expressed miRNAs (DEMs) were found (40 upregulated and 74 downregulated) between two groups before and after transportation. Furthermore, more than 90% of the miRNAs with counts of more than 10 were used to construct a co-expression network by weighted correlation network analysis (WGCNA), and four independent modules were identified. According to their relationship with 30 hub genes, the turquoise module was the key module in this study. The regulator network of hub genes and miRNAs in the turquoise module was constructed by miRNAs targeting genes predicting, and the miRNAs had targeting sites within hub genes that could be identified as hub-miRNAs. Further, it showed that CD40 and ITPKB had the same targeting miRNAs (miR-339a/b), and the newly discovered hub miRNAs filled the gaps in our previous study about the relationship between hub genes in short-distance transportation stress and provided the potential utility for predicting and treatment of short-distance transportation stress in beef cattle.

scholarly journals Transcriptome Characterization of Short Distance Transport Stress in Beef Cattle Blood

2021 ◽  
Vol 12 ◽  
Author(s):  
Haidong Zhao ◽  
Xiaoqin Tang ◽  
Mingli Wu ◽  
Qi Li ◽  
Xiaohua Yi ◽  
...  
Keyword(s):  
Beef Cattle ◽  
B Cell ◽  
Short Distance ◽  
Cell Function ◽  
Density Lipoprotein ◽  
Rna Seq ◽  
Long Distance ◽  
B Cell Function ◽  
Transport Stress ◽  
Transportation Stress

The transportation is a crucial phase in beef cattle industry, and the annual losses caused by beef cattle transport stress are substantial. Several studies have described the effect of long distance transportation stress on animal health, such as disorder in nervous, endocrine, immune, and metabolic system. However, molecular mechanisms underlying short distance transportation stress is still poorly understood. Present study aims to investigate the effect of short distance transportation by measuring the hematological indices and transcriptomic analysis. In this study, a total 10 Qinchuan cattle were used to compare the molecular characteristics of blood before and after transportation. We have found that a stress-related marker “white blood cell count (WBC)” increased significantly after transportation. The decrease in triglyceride (TG), cholestenone (CHO), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) showed that energy expenditure was increased after transportation, but not enough to activate fatty decomposition. Intriguingly, the decrease of malondialdehyde (MDA) showed that cattle were more resilience to oxidative stress. The RNA-seq showed that 1,092 differentially expressed genes (DEGs) were found (329 up-regulated and 763 down-regulated) between group before and group after. The GO and KEGG enrichment showed that the metabolic pathway and B cell function related pathways were enriched. Furthermore, median absolute deviation (MAD) top 5,000 genes were used to construct a co-expression network by weighted correlation network analysis (WGCNA), and 11 independent modules were identified. Combing with protein-protein interaction (PPI) analysis, the verification of quantitative real-time PCR (qPCR) and the correlation of B cell function, structural maintenance of chromosomes 3 (SMC3), jun proto-oncogene (JUN), and C-X-C motif chemokine ligand 10 (CXCL10) were suggested as potential molecular markers in identification of short distance transportation. Collectively, the blood RNA-seq analysis and WGCNA indicated that the disorder of B cell differentiation, proliferation, survival, and apoptosis were the potential molecular mechanism in short distance transportation stress. In conclusion, our results provide the novel insight about potential biomarkers for short distance transportation stress, which may serve as for diagnosing and preventing this condition in beef industry.

scholarly journals Rapid change in host use of the common cuckoo Cuculus canorus linked to climate change

2010 ◽  
Vol 278 (1706) ◽  
pp. 733-738 ◽  
Author(s):  
A. P. Møller ◽  
N. Saino ◽  
P. Adamík ◽  
R. Ambrosini ◽  
A. Antonov ◽  
...  
Keyword(s):  
Climate Change ◽  
Short Distance ◽  
Rapid Change ◽  
Host Use ◽  
Cuculus Canorus ◽  
Common Cuckoo ◽  
Spring Temperature ◽  
Long Distance ◽  
Before And After ◽  
The Common

Parasites require synchrony with their hosts so if host timing changes with climate change, some parasites may decline and eventually go extinct. Residents and short-distance migrant hosts of the brood parasitic common cuckoo, Cuculus canorus , have advanced their phenology in response to climate change more than long-distance migrants, including the cuckoo itself. Because different parts of Europe show different degrees of climate change, we predicted that use of residents or short-distance migrants as hosts should have declined in areas with greater increase in spring temperature. Comparing relative frequency of parasitism of the two host categories in 23 European countries before and after 1990, when spring temperatures in many areas had started to increase, we found that relative parasitism of residents and short-distance migrants decreased. This change in host use was positively related to increase in spring temperature, consistent with the prediction that relative change in phenology for different migrant classes drives host-use patterns. These findings are consistent with the hypothesis that climate change affects the relative abundance of different host races of the common cuckoo.

Erwinia amylovora (fireblight).

2021 ◽  
Author(s):  
Joel Vanneste
Keyword(s):  
New Zealand ◽  
Short Distance ◽  
Western Europe ◽  
Fire Blight ◽  
Erwinia Amylovora ◽  
Rare Event ◽  
Economic Losses ◽  
Plant Tissues ◽  
Long Distance ◽  
Being Moved

Abstract The long distance spread of fire blight is a rare event which in most cases seems to be the result of plants or plant tissues being moved across the oceans. Short distance spread is the result of the characteristics of the pathogen, especially its ability to produce an exudate (bacteria embedded in exopolysaccharides) which is easily transported by wind, rain, insects or birds. This is very efficient; once the pathogen has moved into a new territory it almost always colonizes and becomes established. This is accompanied by economic losses in regions where apple, pear or loquat are grown commercially; it might prevent the survival of local cultivars and could disrupt international trade. To date fire blight has colonized most of North America, Western Europe and most of the countries around the Mediterranean Sea as well as New Zealand. Outbreaks of fire blight are irregular and difficult to control.

scholarly journals K → μ+μ− as a clean probe of short-distance physics

2021 ◽  
Vol 2021 (7) ◽  
Author(s):  
Avital Dery ◽  
Mitrajyoti Ghosh ◽  
Yuval Grossman ◽  
Stefan Schacht
Keyword(s):  
Matrix Element ◽  
Standard Model ◽  
Short Distance ◽  
Time Dependent ◽  
Final States ◽  
Interference Effects ◽  
Fundamental Theory ◽  
Long Distance ◽  
Ckm Matrix ◽  
The Standard Model

Abstract The K → μ+μ− decay is often considered to be uninformative of fundamental theory parameters since the decay is polluted by long-distance hadronic effects. We demonstrate that, using very mild assumptions and utilizing time-dependent interference effects, ℬ(KS → μ+μ−)ℓ=0 can be experimentally determined without the need to separate the ℓ = 0 and ℓ = 1 final states. This quantity is very clean theoretically and can be used to test the Standard Model. In particular, it can be used to extract the CKM matrix element combination $$ \mid {V}_{ts}{V}_{td}\sin \left(\beta +{\beta}_s\right)\mid \approx \mid {A}^2{\lambda}^5\overline{\eta}\mid $$ ∣ V ts V td sin β + β s ∣ ≈ ∣ A 2 λ 5 η ¯ ∣ with hadronic uncertainties below 1%.

scholarly journals Innovative passive reinforcements for the gradual stabilization of a landslide according with the observational method

Landslides ◽  
2021 ◽  
Author(s):  
Lorenzo Brezzi ◽  
Alberto Bisson ◽  
Davide Pasa ◽  
Simonetta Cola
Keyword(s):  
Monitoring System ◽  
Low Cost ◽  
Economic Losses ◽  
Fe Model ◽  
Site Conditions ◽  
Observational Method ◽  
Rainy Period ◽  
Sustainable Solutions ◽  
Before And After ◽  
Set Up

AbstractA large number of landslides occur in North-Eastern Italy during every rainy period due to the particular hydrogeological conditions of this area. Even if there are no casualties, the economic losses are often significant, and municipalities frequently do not have sufficient financial resources to repair the damage and stabilize all the unstable slopes. In this regard, the research for more economically sustainable solutions is a crucial challenge. Floating composite anchors are an innovative and low-cost technique set up for slope stabilization: it consists in the use of passive sub-horizontal reinforcements, obtained by coupling a traditional self-drilling bar with some tendons cemented inside it. This work concerns the application of this technique according to the observational method described within the Italian and European technical codes and mainly recommended for the design of geotechnical works, especially when performed in highly uncertain site conditions. The observational method prescribes designing an intervention and, at the same time, using a monitoring system in order to correct and adapt the project during realization of the works on the basis of new data acquired while on site. The case study is the landslide of Cischele, a medium landslide which occurred in 2010 after an exceptional heavy rainy period. In 2015, some floating composite anchors were installed to slow down the movement, even if, due to a limited budget, they were not enough to ensure the complete stabilization of the slope. Thanks to a monitoring system installed in the meantime, it is now possible to have a comparison between the site conditions before and after the intervention. This allows the evaluation of benefits achieved with the reinforcements and, at the same time, the assessment of additional improvements. Two stabilization scenarios are studied through an FE model: the first includes the stabilization system built in 2015, while the second evaluates a new solution proposed to further increase the slope stability.

PSVIII-5 Genetic parameters of thrombin as a proxy for horn fly abundance in beef cattle

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 239-239
Author(s):  
Ashley S Ling ◽  
Taylor Krause ◽  
Amanda Warner ◽  
Jason Duggin ◽  
Bradley Heins ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Fixed Effects ◽  
Large Scale ◽  
Genetic Basis ◽  
Threshold Model ◽  
Economic Losses ◽  
Small Data ◽  
Horn Fly ◽  
Improvement Programs ◽  
Highest Posterior Density

Abstract Horn flies (Haematobia irritans) are a major nuisance to cattle, especially in warm, humid regions, and are estimated to cause economic losses in excess of $1 billion annually to the U.S. beef cattle industry. Variation in horn fly tolerance has been reported within and across breeds, and heritability estimates ranging between 10 and 80% show a clear genetic basis. However, collecting fly abundance phenotypes is costly and logistically demanding, which precludes large-scale implementation. Consequently, finding correlated phenotypes and endo-phenotypes that are heritable and relatively easy to measure would facilitate implementation of horn fly tolerance genetic improvement programs. Thrombin (TH), a blood coagulation precursor, has a reported association with horn fly count variation within and across cattle breeds. In this study, the genetic basis of thrombin in beef cattle was investigated. Blood samples and horn fly count were collected on 360 cows and heifers twice during the summer of 2019 (June and August). Due to uncertainty associated with assessment of horn fly abundance and thrombin and the fact that economic losses occur only when fly abundance exceeds a certain threshold, thrombin was categorized into 4 classes (1=TH > 500 ng/ml; 2=250< TH< 500 ng/ml; 3=100< TH< 250 ng/ml; and 4=TH< 100 ng/ml). The trait was analyzed using linear (continuous) and threshold (discrete) mixed models. Both models included farm, pregnancy status, and cow age as fixed effects and additive and permanent environment random effects. The pedigree included 642 animals. Estimates of heritability were 0.24 and 0.29 using linear and threshold models, respectively. Estimates of repeatability were slightly higher using the threshold model (0.21 vs 0.19). Despite the small data size, all estimates were non-zero based on their respective highest posterior density intervals. These results indicate reasonable genetic variation for thrombin that could be harnessed for improvement of horn fly tolerance in cattle.

scholarly journals Utility of acute phase proteins as biomarkers of transport stress in ewes and beef cattle

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Francesco Fazio ◽  
Vincenzo Ferrantelli ◽  
Antonello Cicero ◽  
Stefania Casella ◽  
Giuseppe Piccione
Keyword(s):  
Beef Cattle ◽  
Repeated Measures ◽  
Acute Phase Proteins ◽  
Animal Health ◽  
White Blood Cells ◽  
Amyloid A ◽  
Repeated Measures Analysis ◽  
Before And After ◽  
Rest Time ◽  
Transport Stress

The effect of transport on serum amyloid A (SAA), haptoglobin (Hp), Fibrinogen and white blood cells (WBC) was evaluated in 10 ewes and 10 beef cattle. All animals were transported by road for 6 h over a distance of about 490 km with an average speed of 80 km/h. Blood samples, collected via jugular venepuncture, were obtained before and after transport as well as after 12, 24 and 48 h rest time. One-way repeated measures analysis of variance showed a statistically significant effect of sampling time on SAA, Hp, and WBC in ewes and beef cattle. Based on these results, Hp and SAA levels, together with WBC, may be useful indicators of animal health and welfare and in predicting the risk assessment in meat inspection.

scholarly journals Identifikasi Keragaman Gen FTO Pada Bangsa Sapi Potong Indonesia

2019 ◽  
Vol 6 (2) ◽  
pp. 232
Author(s):  
Sutikno Sutikno ◽  
Rudy Priyanto ◽  
Cece Sumantri ◽  
Jakaria Jakaria
Keyword(s):  
Beef Cattle ◽  
Genetic Markers ◽  
Direct Sequencing ◽  
Nucleotide Position ◽  
Fto Gene ◽  
Blood Samples ◽  
Pcr Rflp ◽  
Gene Functions ◽  
Hardy Weinberg Equilibrium ◽  
Cattle Blood

ABSTRAK Gen FTO berfungsi sebagai regulasi homeostasis, deposisi lemak dan pengaturan obesitas. Penelitian ini bertujuan untuk mengidentifikasi polimorfisme SNP g.125550A>T di ekson 3 gen FTO pada bangsa sapi potong Indonesia. Sampel darah diperoleh dari 209 ekor sapi, terdiri atas sapi bali (44), madura (20), Pesisir (20), katingan (20), Peranakan ongole (PO) (22), Pasundan (20), Sumba Ongole (SO) (11), brahman (20), simental (15), dan limousin (18). Polimorfisme gen FTO dianalisis menggunakan metode PCR-RFLP (HpyCH4III) dan direct sequencing. Hasil genotiping SNP g.125550A>T adalah polimorfik (genotipe AA, AT, dan TT) pada sapi madura, pesisir, katingan, PO, pasundan, SO, brahman, simental, dan limousin. Frekuensi alel A dan T masing-masing adalah 0,70, 0,68, 0,84, 0,89, 0,70, 0,86, 0,90, 0,73, 0,69 dan 0,30, 0,33, 0,16, 0,11, 0,30, 0,14, 0,10, 0,27, 0,31. Nilai Ho dan He masing-masing adalah 0,60-0,14 dan 0,44-0,18 serta dalam keseimbangan Hardy-Weinberg (P>0.05). Sementara pada sapi bali bersifat monomorfik hanya bergenotipe AA. Hasil sekuensing SNP g.125550A>T ditemukan mutasi tranvesi A menjadi T pada posisi nukleotida  g.125550. Berdasarkan hasil penelitian ini, dapat disimpulkan bahwa SNP 125550A>T gen FTO beragam dan berpotensi dijadikan marka genetik untuk kualitas daging pada bangsa sapi potong Indonesia.Kata Kunci: gen FTO, PCR-RFLP, Sapi, SNP g.125550A>TABSTRACTThe FTO gene functions as regulation of homeostasis, fat deposition and regulation of obesity. This study aimed to identify the polymorphism of SNP g.125550A>T in exon 3 of FTO gene in Indonesian beef cattle. Blood samples were collected from 209 cattle, including bali (44), madura (20), pesisir (20), katingan (20), PO (22), pasundan (20), SO (11), brahman (20), simental (15), and limousin (18). Polymorphism of the FTO gene was analyzed using PCR-RFLP (HpyCH4III) and direct sequencing methods. The results of genotyping SNP g.125550A>T was polymorphic (AA, AT and TT genotypes) in madura, pesisir, katingan, PO, pasundan, SO, brahman, simental, and limousin cattle. The frequency of A and T alleles were 0,70, 0,68, 0,84, 0,89, 0,70, 0,86, 0,90, 0,73, 0,69 and 0,30, 0,33, 0,16, 0,11, 0,30, 0,14, 0,10, 0,27, 0,31 respectively. The values of Ho and He were 0,60-0,14 and 0,44-0,18 respectively and in Hardy-Weinberg equilibrium (P>0,05). While in Bali cattle was monomorphic (AA genotype). Results of sequencing SNP g.125550A>T of the FTO gene found a transverse mutation A to T at the nucleotide position g.125550. As a result of this study, it can be concluded that SNP 125550A>T of the FTO gene was diverse and potentially used as genetic markers for meat quality in Indonesian beef cattle.Keywords: cattle, FTO gene, PCR-RFLP, SNP g.125550A>T.

scholarly journals Energy drink - the prevalence of consumption and awareness regarding its associated potential health problems among commercial bus drivers in Ho, Ghana

2020 ◽  
Author(s):  
Emmanuella Yayra Saku ◽  
Peter Nuro-Ameyaw ◽  
Priscilla Cecilia Amenya ◽  
Fidelis Mawunyo Kpodo ◽  
Paul Esua Amoafo ◽  
...  
Keyword(s):  
Side Effects ◽  
Public Health Problem ◽  
Energy Drinks ◽  
Energy Drink ◽  
Health Problems ◽  
Bus Drivers ◽  
Long Distance ◽  
Potential Health ◽  
Poor Knowledge ◽  
Energy Drink Consumption

Abstract Background Consumption of energy drinks has become an escalating global public health problem. The work schedule and irregular sleeping habits of commercial bus drivers make them highly susceptible to getting fatigued, hence most of them consume energy drinks as a fatigue management strategy. However, consumption of energy drinks produces numerous psychomotor side effects that if consumed among drivers puts the traveling public in danger of road accidents. This study sought to assess the prevalence of energy drink consumption and awareness of associated potential health problems among commercial long-distance bus drivers operating from the Ho municipality. Methods This was a cross-sectional study involving 132 participants who completed a structured questionnaire on the participants' socio-demographic characteristics, frequency of consumption and reasons for consumption. It also included questions to assess the knowledge of the ingredients and side effects of energy drinks. Results A majority (62.1%) of the drivers had more than 10 years of commercial driving experience. A 75% energy drink consumption prevalence was recorded with driving performance enhancement (78.8%) as the predominant reason for consumption. 7 - 10 bottles per week were consumed by most (32.2%) of the drivers with the most consumed brand being Rush energy drink (54.5%). Also, 72.0% had poor knowledge of the side effects linked with energy drink intake likewise the ingredients in them. Conclusion Energy drinks were consumed by the majority of the drivers at the Ho main bus terminal of which most of the drivers had poor knowledge of the potential health problems linked with the consumption of these drinks. The consumption of energy drinks was observed to be higher among the drivers with lower education levels, higher monthly income and those who worked long hours in a day. The Ghana National Road Safety Commission (GNRC) in collaboration with other private road transport unions in Ghana should organize regular seminars for commercial bus drivers on the potential dangers and effects associated with energy drink consumption.

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