scholarly journals Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (Silurus triostegus, H. 1843) Sperm

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3029
Author(s):  
Zafer Doğu ◽  
Erdinç Şahinöz ◽  
Faruk Aral ◽  
İsmail Koyuncu ◽  
Özgür Yüksekdağ
Keyword(s):  
Dna Damage ◽  
Semen Quality ◽  
Spermatozoa Motility ◽  
Positive Effects ◽  
Total Antioxidant ◽  
High Doses ◽  
Oxidant Status ◽  
Motility Rate

This study was based on the determination of post-thaw semen quality, oxidant–antioxidant status and DNA-damage status of frozen Mesopotamian catfish semen. To this end, the sperm was frozen in diluent containing different inositol concentrations (5, 10, 20 and 40 mg). Increasing levels of inositol linearly increased the spermatozoa motility rate and duration significantly (p < 0.05). High doses of inositol increased antioxidants and decreased oxidants. In addition, lower intracellular DNA damage and percentage of apoptotic spermatozoa occurred in high doses of inositol (p < 0.05).Abstract: In this study, the effects of supplemented inositol on sperm extenders were examined on the spermatozoa motility rate and duration, total antioxidant and oxidant status, apoptotic spermatozoa and DNA damage, during the sperm post-thaw process of Mesopotamian Catfish (Silurus triostegus, H. 1843). The semen was frozen in diluents containing different inositol concentrations (5, 10, 20 and 40 mg). Increasing levels of inositol linearly improved the spermatozoa motility rate and duration significantly (p < 0.05). MDA and TOS were linearly decreased, however, TAS and GSH linearly increased (p < 0.05). The increasing inositol levels resulted in a linear and quadratic decrease in DNA damage in the comet assay, 8-hydroxydeoxyguanosine and the determined percentage of apoptotic spermatozoa (p < 0.05). These results suggest that there are many positive effects of the use of supplemental inositol on enhancing sperm cryopreservation efficiency in Silurus triostegus.

scholarly journals Effects of chlorpyrifos on primary gill cell culture of Lake Van fish (Alburnus tarichi Güldenstaadt 1814)

2020 ◽  
Vol 9 (6) ◽  
pp. 741-745
Author(s):  
Ahmet Regaib Oğuz ◽  
Elif Kaval Oğuz ◽  
Necati Özok
Keyword(s):  
Cell Culture ◽  
Dna Damage ◽  
Total Antioxidant Status ◽  
Toxic Substances ◽  
Lake Van ◽  
Gill Cell ◽  
Total Antioxidant ◽  
High Doses ◽  
Oxidant Status ◽  
Different Doses

Abstract Lake Van fish (Alburnus tarichi Guldenstadt 1814) is the only fish that can adapt to the extreme conditions (pH 9.8 salinity 0.2% and alkalinity 151.2 meq/L) of Lake Van. In this study, it was aimed to determine the cytotoxic and genotoxic effects of chlorpyrifos (CPF) on Lake Van fish primary gill cell culture. Gill epithelium from Lake Van fish was isolated enzymatically and grown in primary culture on Leibovitz’s L-15 medium. After different doses (0.01, 0.1, 1, and 10 μM) of CPF were applied to the gill cells, the total oxidant status (TOS), total antioxidant status (TAS), malondialdehyde (MDA), and DNA damage levels (8-hydroxyguanine (8-OHdG)) were examined at the end of 24 and 48 h. It was determined that the TOS, MDA, and 8-OHdG levels increased in the cells exposed to high doses (1 and 10 μM) of CPF and the TAS was decreased (P &lt; 0.05). It was revealed from this study that CPF administered at a dose higher than 1 μM can cause oxidative stress and DNA damage in the primary gill cell culture of Lake Van fish. In addition, the findings showed that Lake Van fish primary gill cell culture was useful in determining the effects of toxic substances likely to be the contaminants of a lake.

scholarly journals Once-a-day Tadalafil administration improves the spermogram parameters in fertile patients

2015 ◽  
Vol 87 (3) ◽  
pp. 210 ◽  
Author(s):  
Antonio Corvasce ◽  
Giuseppe Albino ◽  
Teresa Leonetti ◽  
Agnese Filomena Buonomo ◽  
Ettore Cirillo Marucco
Keyword(s):  
Seminal Fluid ◽  
Semen Quality ◽  
Type A ◽  
Daily Treatment ◽  
Sperm Cells ◽  
Average Increase ◽  
Once Daily ◽  
Positive Effects ◽  
Semen Volume

Objectives: We explored the safety of Tadalafil once-daily treatment for 12 week and its effects on semen quality in a clinical subpopulation of men with psychogenic erectile dysfunction (ED). Patients and Methods: Twenty-seven men, between 19 and 35 years, unaware of their fertility status, suffering from psychogenic ED were enrolled. The examination of the seminal fluid was performed twice before administration of Tadalafil and twice after three months of continuous daily administration of Tadalafil 5 mg. The volume of the seminal fluid, the concentration of sperm, the concentration of sperm with type “a+b” motility, the concentration of sperm with type “a” motility, the concentration of normal sperm were taken into consideration. The results before treatment with Tadalafil (T0) and after 3 months of treatment (T3m) were compared. Results: The administration of once-daily Tadalafil 5mg, brings to an average increase of the total number of sperm cells, both total and fast motility (type a) and the percentage of nemasperms, and to an average increase of semen volume of only 0.41 ml. These quantitative and qualitative improvements of the seminal fluid resulted statistically significant as regard motility, nemasperm percentage and seminal fluid volume. No unespected safety findings were observed. Conclusions: Tadalafil administration improves the quality of sperm cells and seminal fluid: in particular motility, percentage of nemasperms and volume of seminal fluid. We emphasize the safety of the once-daily treatment with tadalafil 5mg and the positive effects on spermatogenesis.

scholarly journals KUALITAS SEMEN SAPI PEJANTAN SIMMENTAL DAN LIMOUSIN YANG DIPELIHARA DALAM TIPE KANDANG YANG BERBEDA DI BALAI INSEMINASI BUATAN LEMBANG

ZOOTEC ◽  
2020 ◽  
Vol 40 (2) ◽  
pp. 439
Author(s):  
Bastiana Elvira Lagu ◽  
Endang Pudjihastuti ◽  
Umar Paputungan ◽  
Sri Adiani
Keyword(s):  
High Speed ◽  
Semen Quality ◽  
Mass Movement ◽  
Secondary Data ◽  
Test Method ◽  
Spermatozoa Motility ◽  
Different Types ◽  
Animal House ◽  
Better Than

SEMEN QUALITY OF LIMOUSIN AND SIMMENTAL BULLS MAINTAINED IN DIFFERENT TYPES OF PENS AT THE INSTITUTION OF LEMBANG ARTIFICIAL. The intensive maintenance of bulls included animal house and pens protecting animals against sunlight, cool effect, rain, high speed of win and other predators. Objective of this study was to evaluate effects of single bull in a pen (SB) and double bulls in a pen (DB) on semen quality of Limousin and Simmental bulls at the Institution of Lembang Artificial Insemination in West Java. Primer and secondary data of both bulls reproduction were used in this study and analyzed by t test method. Results showed that average volume of semen in both bulls of Limousin and Simmental under SB (9.54 ± 2.19 mL/ejaculation) was significantly higher of 7.67 percents than those of bulls under DB (8.86 ± 1.79 mL/ejaculation). The average of percentage of spermatozoa motility in both bulls of Limousin and Simmental under DB (71.00 ± 2.67) was not significantly differ with those of bulls under SB (70.10 ± 2.37). The average of spermatozoa mass movement in both bulls of Limousin and Simmental under SB (1.84 ± 0.37) was significantly more aggressive of 5.43 percents than those of bulls under DB (1.94 ± 0.24). Finally, the average of pH of semen in both bulls of Limousin and Simmental under SB (6.66 ± 0.11) was not significantly differ with those of bulls under DB (6.64 ± 0.10). Correlation between reproduction variables of both Limousinand Simmental bulls in SB and DB was not correlated between one and another variables indicated by correlation values ranging from -0.29 to 0.18. Based of the result of study can be concluded that semen quality of Limousin and Simental in single pen was better than that in double pens. The correlations among reproductive veriables of Limousin and Simmental in both sigle and double pens had no correlation between one and another variable. Keyword: Type of Pen, Semen Quality, Simmental Bull, Limousin Bull.

scholarly journals The Influence of Breed and Type of Extender on the Quality of Bull Semen

2020 ◽  
Vol 21 (2) ◽  
pp. 64
Author(s):  
Iman Sukirman ◽  
Eros Sukmawati ◽  
Siti Darojah Rasad ◽  
Nurcholidah Solihati
Keyword(s):  
Plasma Membrane ◽  
Semen Quality ◽  
Block Design ◽  
Egg Yolk ◽  
Spermatozoa Motility ◽  
Bull Semen ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Two Factors

This study aimed to determine the influence of breed and type of extenders on frozen semen quality of cows at BIB Lembang. The experimental study was conducted in a Factorial Randomized Block Design (RBD) with two factors. The first factor was four cow breeds, i.e. Ongole Cross (PO), Brahman (BR), Simmental (SM) and Limousin (LM), and the second factor was two types of extender, i.e. Skim-Egg Yolk (SKT) and AndroMed® (AND), all repeated four times. The observed variables were percentage of spermatozoa motility and intact plasma membrane (IPM). All data obtained were analyzed using a general linear model (IBM SPSS ver. 23). The results demonstrated an interaction between breed and the type of diluent to motility. Breeds showed significantly different motility but non-significantly different intact plasma membrane (MPU) of semen. The type of diluent did not significantly affect motility and intact plasma membrane (MPU) of the frozen semen. The effect of the breed on BR motility was lower and significantly different from PO, LM and SM. The types of diluent did not significantly affect motility, MPU. The results showed that SKT was lower than AND, it was indicative effect of breed on intact plasma membrane (MPU) PO was lower than BR, LM and SM and the effect of the type of diluent on whole plasma membrane (MPU) AND is lower than SKT. It can be concluded that breed influences the motility of semen. The lowest motility reduction in frozen semen is Brahman cattle by using skim-egg yolk extender.

scholarly journals Hubungan Lingkar Skrotum dengan Kualitas Semen Sapi Kuantan Riau

2019 ◽  
Vol 3 (1) ◽  
pp. 19-24
Author(s):  
Jiyanto Jiyanto ◽  
Pajri Anwar
Keyword(s):  
Regression Analysis ◽  
Semen Quality ◽  
Regression Equation ◽  
Moderate Correlation ◽  
Spermatozoa Motility ◽  
Scrotal Circumference ◽  
Low Correlation ◽  
The Relationship

The aim of this study was to determine the correlation between scrotal circumference and semen quality of the Riau Kuantan cattle. The study was conducted in Benai District, Kuantan Singingi Regency. The material used was 15 Kuantan bulls. The parameters observed were scrotal circumference, semen quality and the correlation between scrotal circumference and semen quality. The results of this study indicated that the size of Kuantan cattle scrotum circumference was 23.06 cm; viability 76.67%; and motility 58%. The results of regression analysis which conducted on scrotal circumference for the viability and motility showed not significant (P0.05). The correlation between scrotal circumference with spermatozoa viability of Kuantan cattle showed a moderate correlation of 0.40. The relationship between scrotal circumference with spermatozoa motility in Kuantan cattle showed a low correlation of 0.22. The regression equation obtained to determine the relationship between scrotal circumference with viability and motility of spermatozoa were Y = 45.44 + 1.35X and Y = 44.77 + 0.57X, respectively. The results of this study indicated that scrotal circumference cannot be made as determinant of spermatozoa quality of in Riau Kuantan bulls.

scholarly journals KUALITAS SPERMATOZOA AYAM PERANAKAN SENTUL DALAM PENGENCER RINGER LAKTAT KUNING TELUR DENGAN BERBAGAI MONOSAKARIDA (Quality of Sentul Crossbreed Chicken Spermatozoa in Ringer Lactate-Egg Yolk Diluents Supplemented with Various Monosaccharide)

2016 ◽  
Vol 10 (2) ◽  
pp. 166-169
Author(s):  
Khaeruddin Khaeruddin ◽  
Raden Iis Arifiantini ◽  
Cece Sumantri ◽  
Sri Darwati
Keyword(s):  
Energy Source ◽  
Semen Quality ◽  
Egg Yolk ◽  
Quality Parameters ◽  
Ringer Lactate ◽  
Spermatozoa Motility ◽  
Significant Difference

The aim of this study was to examine the preservation of sentul crossbreed chicken semen in ringer lactate egg yolk diluent supplemented with various monosaccharide. Semen was collected from three roosters using massage method. Immediately after collection, the semen was evaluated macroscopically and microscopically. Semen with more than 70% motility was divided into four tubes. Each of them diluted with ringer lactate egg yolk glucose (RLEYG), ringer lactate egg yolk fructose (RLEYF), ringer lactate egg yolk xylose (RLEYX) and ringer lactate egg yolk mannose (RLEYM). Semen was stored in refrigerator (5o C) for sixty hours and evaluated every twelve hours for spermatozoa motility and viability. Results showed that no significant difference (P>0.05) among diluents used on spermatozoa quality parameters after dilution and during preservation. Semen quality decrease during storage and at sixty hours of storage, the motility and viability of spermatozoa ranging from 48.33±2.56 to 55.42±2.26% and 58.59±2.87 to 64.83±2.42%, respectively. This research conclude that glucose, fructose, xylose and mannose can be used as energy source for roosters semen during preservation.

scholarly journals The Effect of the Separation of Spermatozoa X and Y Using Swim up Method on the Quality of Etawah Crossbreed Goats Spermatozoa

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Eskayanti Pasaribu ◽  
Dasrul Dasrul ◽  
Ginta Riady
Keyword(s):  
Semen Quality ◽  
Control Group ◽  
Spermatozoa Motility ◽  
Treatment Groups ◽  
Multiple Test ◽  
Group 3 ◽  
The Mean ◽  
Group 2 ◽  
Method Group

This research aimed to determine the effect of spermatozoa X and Y separation using swim-up method on the quality of etawah crossbreed goat’s spermatozoa. This research used 6 male etawah crossbreed goats aged between 18-24 months, which were collected the semen once a week using electroejaculator. Immediately after semen collection, the quality of semen was examined, and then grouped into three treatment groups. Group 1 was control group (P0) refers to the semen was not separated by swim-up method. Group 2 (P1) was the semen separated by the swim-up method for 5 minutes. Group 3 (P2) was the semen separated by swim-up method for 10 minutes. Each treatment was repeated six times. Parameters for semen quality examined were the percentage of spermatozoa motility, live spermatozoa, and abnormal spermatozoa. Data were analyzed by analysis of variance (ANOVA) one way pattern, followed by Duncan's multiple test. The results showed that the mean ± SD percentage of sperm motility on each treatment groups (P0; P1; P2) was 81.33±3.44, 89.67±3.21, and 90.00±3.10 %, respectively. The percentage of live spermatozoa on each group was 86.50 ± 2.07, 92.33 ± 2.08, and 91.83 ± 1.72%, respectively. The percentage of abnormal spermatozoa on each group was 13.00±2.53, 7.33±2.52, and 7.67±2.16%, respectively. Results of this research showed that the percentages of motility, live spermatozoa, and abnormal spermatozoa after separation by swim-up method were significantly different (P0.05) compared to without separation. It can be concluded that the separation of spermatozoa using swim-up method can improve significantly (P0.05) the percentage of live and motility of spermatozoa and decrease the percentage of abnormal spermatozoa of etawah crossbreed goats.Key words: Etawah crossbreed, spermatozoa quality, swim-up method

scholarly journals The Effect of Centrifugation on Semen Quality of Peranakan Ettawah Goat's Post Thawing

2010 ◽  
Vol 4 (2) ◽  
Author(s):  
Indiah Indiah ◽  
Sri Wahjuningsih
Keyword(s):  
Semen Quality ◽  
Lower Layer ◽  
Vitro Fertilization ◽  
Spermatozoa Motility ◽  
Tukey Test ◽  
Abnormal Morphology ◽  
Frozen Semen ◽  
Experiment Method

The aim of the research was to determine the optimum of centrifugation on the quality of Peranakan Ettawah goat's spermatozoa prepared for in vitro fertilization preparation.The research of material was frozen semen ofPeranakan Ettawah goat produced by Artificial Insemination Center in Singosari with minimum post thawing motility in 40%. The method of the research was an experiment method with the four treatment, i.e: 1000 (P1), 1500 (P2), 2000 rpm (P3) of different centrifugation. The variable observes were spermatozoa motility, spermatozoa viability, and abnormal morphology of spermatozoa. Data obtain was analyzed statistically using Completely Randomize Design and continued with Tukey test. The result showed that on the upper layer of each treatment (P1, P2, and P3) obtained 55,72±3,55; 69,55±3,35; and 55,19±2,72% for spermatozoa motility, 62,99±5,87; 73,99±4,36; and 57,39±9,22% for spermatozoa viability, and 7,19±3,64; 8,84±2,65; and 6,40±3,00% for abnormal morphology of spermatozoa, descriptively. While on the lower layer the result showed 55,59±3,99; 68,63±3,88; and57,61±2,20% for spermatozoa motility, 70,05±7,47; 77,44±1,08; and 69,93±11,98% for spermatozoa viability, and 10,36±6,20; 9,55±3,27; and 8,09±2,80% for abnormal morphology of spermatozoa, desciptively. It was concluded that centrifugation in 1500 rpm showed the highest motility and viability on the upper layer and lower layer.

scholarly journals Quality of semen in the reproductive cycle of Cachara (Pseudoplatystoma fasciatum) raised in captivity

2019 ◽  
Vol 41 ◽  
pp. e46517
Author(s):  
Rodrigo Diana Navarro ◽  
Juliana Ventorim Lemos ◽  
Marcela Tosta Ribeiro
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Reproductive Period ◽  
Significant Difference ◽  
In Captivity ◽  
Intermediate Part ◽  
Mean Concentration ◽  
Evident Relationship ◽  
Motility Rate

In this study, we evaluated the semen quality of Cachara (Pseudoplatystoma fasciatum) raised in captivity during its reproductive period. For the evaluation of qualitative parameters, we analyzed: sperm morphology, sperm motility, and sperm vigor; while for the quantitative parameter, we analyzed sperm concentration. In general, the main significant difference was found in March, with a motility rate of 40% and a duration of 39 seconds. The highest mean concentration (1.2x10⁸) was observed in February, as well as the highest percentage of defects in the intermediate part of the spermatozoa (59%). The morphological analysis of spermatozoa showed an average of 24.2% abnormalities in the head and 22.7% in the intermediate part of spermatozoa of P. fasciatum for the four months studied. We did not find any evident relationship between the climate changes evaluated and the qualitative and quantitative parameters. The greatest difference between the percentage of normal spermatozoa and the sharp increase of defects in the intermediate piece, observed in February, can be explained by the sudden climate change, with a significant decrease in temperature and relative humidity, and increase in precipitation. These findings demonstrate the importance of maintaining an optimum climate interval during the breeding season for P. fasciatum.

scholarly journals Thermo-Ultrasound-Based Sterilization Approach for the Quality Improvement of Wheat Plantlets Juice

Processes ◽  
2019 ◽  
Vol 7 (8) ◽  
pp. 518 ◽  
Author(s):  
Zahoor Ahmed ◽  
Muhammad Faisal Manzoor ◽  
Nabila Begum ◽  
Abbas Khan ◽  
Imranullah Shah ◽  
...  
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Titratable Acidity ◽  
Viable Option ◽  
Untreated Sample ◽  
Total Antioxidant ◽  
The Impact

The impact of thermo–ultrasound (TU) on the quality of fresh wheat plantlets juice is described in this study. Fresh wheat plantlets juice was treated with TU using ultrasound (US) bath cleaner with different treatment variables, including power (70%, 420 W), frequency (40 kHz), processing time (20 and 40 min) and temperature (30, 45 and 60 °C) for the determination of free amino acids, minerals, microbial loads and bioactive compounds. The treatments have non-significant effects in ºBrix, pH, and titratable acidity while a significant increase in non-enzymatic browning, viscosity, and cloud value. The TU treatment at 30 °C for 20 and 40 min has achieved the highest value of total phenolics, flavonoids, total antioxidant capacity, 2, 2-diphenyl-1-picrylhydrazyl (DPPH), carotenoids, anthocyanin contents, chlorophyll (a + b), minerals and free amino acids than other treatments as well as untreated sample. A lightly visible variation in the color was observed among all treatments. TU treatments also showed a significant impact on the reduction of microbial loads at 60 °C for 40 min. The verdicts revealed that TU at low temperature a viable option to improve the quality of wheat plantlets juice at an industrial scale as compared to alone.

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