scholarly journals Impacts of Enriching Growing Rabbit Diets with Chlorella vulgaris Microalgae on Growth, Blood Variables, Carcass Traits, Immunological and Antioxidant Indices

Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 788 ◽  
Author(s):  
Abdelnour ◽  
Sheiha ◽  
Taha ◽  
Swelum ◽  
Alarifi ◽  
...  
Keyword(s):  
Body Weight ◽  
Chlorella Vulgaris ◽  
Antioxidant Status ◽  
Carcass Traits ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Treatment Groups

This work aimed to explore the effects of dietary supplementation of Chlorella vulgaris (CLV) on the growth performance, carcass traits, hematobiochemical variables, immunity responses, and the antioxidant status of growing rabbits. A total number of 100 rabbits were randomly distributed into four treatment groups, each of five replicates (25 rabbits/group). The experimental groups were as follows; control: a basal diet without supplementation, CLV0.5: basal diet + 0.5 g chlorella powder/kg diet; CLV1.0: basal diet + 1.0 g chlorella powder/kg diet, CLV1.5: basal diet + 1.5 g chlorella powder/kg diet. Live body weight (LBW), cumulative body weight gain (CBWG), feed intake (FI), and feed conversion ratio (FCR) were not affected by dietary CLV supplementation. Platelet count (PLT), hematocrit (HCT), means corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) values were significantly increased in the CLV0.5 group compared with the other treatment groups. Dietary supplementation of CLV (1.5 g/kg diet) significantly reduced the alanine aminotransferase (ALT) activity. The concentrations of serum triglycerides and very low-density lipoprotein (VLDL) were lower (p < 0.05) in the CLV-treated groups than those of the control. Supplemental CLV at all experimental levels gave the best values of immunoglobulins (IgG and IgM) and glutathione activities. Malondialdehyde (MDA) levels were lower in the animals that received CLV in their diet than those of the control group. Dietary supplementation of 1.0 g CLV/kg had the potential to enhance immune responses and antioxidant status, as well as reduce blood lipid accumulation. Therefore, it could be concluded that CLV supplementation to growing rabbit diets can improve the health status.

scholarly journals 14. Effect of Dietary Supplementation of Garlic (Allium Sativum) and Turmeric (Curcuma Longa) on Growth Performance, Carcass Traits, Blood Profile and Oxidative Status in Growing Rabbits

2016 ◽  
Vol 16 (2) ◽  
pp. 489-505 ◽  
Author(s):  
Mahmoud Alagawany ◽  
Elwy Ali Ashour ◽  
Fayez Mohamed Reda
Keyword(s):  
Growth Performance ◽  
Control Diet ◽  
Carcass Traits ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Rabbit Serum ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Growth Promoter ◽  
Blood Profile

Abstract This study was performed to investigate the effects of dietary supplementation with garlic and turmeric powder as growth promoter agents on performance, carcass traits, serum biochemistry, and antioxidant enzyme activities of growing rabbits. A total of 112 New Zealand White rabbits (NZW) at 5 weeks of age were randomly assigned to seven treatments with four replicates. The dietary treatments consisted of 7 groups as follows; the basal diet as control, phytogenic additives groups were supplemented with 2, 4, and 6 g/kg garlic or turmeric powder added to the basal diet. There were no linear and quadratic differences (P<0.05) in growth performance after garlic or turmeric supplementation at all studied ages. Compared with the control group, supplementation of diets with garlic or turmeric linearly and quadratically elevated immunity biomarkers such as total protein (TP), albumin (AL) and immunoglobulin (IgG) levels and decreased (linearly and quadratically, P<0.05) aspartate aminotransferase (AST), alanine aminotransferase (ALT ), triglyceride (TG) and total cholesterol (TC) levels in rabbit serum. However, TP, AL, globulin (GL), IgG and IgM levels were linearly and quadratically enhanced with increasing turmeric levels versus the control diet. Hepatic superoxide dismutase (SOD), catalase (CAT ) and glutathione peroxidase GSH-Px activities as well as reduced glutathione (GSH) concentrations were linearly and quadratically (P<0.05) improved in garlic or turmeric additives fed groups. While MDA concentration was statistically (linearly, P= 0.022) reduced in comparison with the control group. It could be concluded that garlic or turmeric supplementation (2, 4 and 6 g/kg) did not linearly and quadratically affect growth performance but improved the immunity responses and lowered the lipid profile in blood and lipid peroxidation in liver and increased hepatic antioxidant activity in treated rabbits.

scholarly journals Administration of garlic and neem in broiler diet for safe meat production

2020 ◽  
Vol 48 (2) ◽  
pp. 116-126
Author(s):  
R Islam ◽  
MM Hossain ◽  
F Nargis ◽  
ME Hossain
Keyword(s):  
Body Weight ◽  
Basal Diet ◽  
Feed Additives ◽  
The Body ◽  
The Other ◽  
Control Group ◽  
Meat Production ◽  
Broiler Chicks ◽  
Treatment Groups ◽  
Broiler Production

This study was conducted to observe the effect of different administration method of garlic and neem on growth performances, feed efficiency and serum biochemical parameters for safe broiler production. The experiment was done for a period of 32 days with 384 one-day-old straight run broiler chicks. The broiler chicks were divided into six groups each of 64 birds and replicated to four subgroups each of 16 birds. The dietary groups were; control (basal diet; no additives), antibiotic (basal diet + antibiotic), garlic in feed (basal diet + 0.25% garlic powder), garlic in water (basal diet + garlic extracts), neem in feed (basal diet + 0.25% neem powder) and neem in water (basal diet + neem extracts). Results showed that the body weight and body weight gain increased significantly (P<0.05) in both the garlic and neem groups compared to the control group. Feed intake was not different (P>0.05) among the treatment groups. Garlic in feed and water and neem in feed groups showed better FCR (P<0.05) compared to the other treatment groups. There were no significant (P>0.05) difference in meat yield, bone development and dressing parameters except head and gizzard in different dietary groups. Supplementation of garlic with feed and water significantly (P<0.05) decrease cholesterol, triglyceride and LDL compared to the other groups. However, both the garlic and neem groups showed numerically higher HDL compare to the control group. There were no 0significant (P>0.05) differences in serum glucose and GPT among different treatment groups. GOT was significantly (P<0.05) lower in neem groups than that of garlic and antibiotic groups. Additive groups showed higher profitability than control group. Based on the results of the study, it may be suggested that the garlic and neem could be used both in feed and water as potential feed additives for safe broiler production. Bang. J. Anim. Sci. 2019. 48 (2): 116-126

scholarly journals Dietary Cold Pressed Watercress and Coconut Oil Mixture Enhances Growth Performance, Intestinal Microbiota, Antioxidant Status, and Immunity of Growing Rabbits

Animals ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 212 ◽  
Author(s):  
Mahmoud Alagawany ◽  
Mohamed Abd El-Hack ◽  
Adham Al-Sagheer ◽  
Mohammed Naiel ◽  
Islam Saadeldin ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Antioxidant Status ◽  
Pathogenic Bacteria ◽  
Coconut Oil ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Control Group ◽  
Feed Conversion ◽  
Live Body Weight ◽  
Cold Pressed

The present study assessed the effect of dietary supplementation with coconut oil (CNO), watercress oil (WCO), and their mixture as promoters of growth, antioxidant status, immunity, and intestinal microbiota in growing rabbits. A total of 120 rabbits were distributed into six groups (20 rabbits/group) receiving a basal diet without supplementation (G1) or diet supplemented with 2 g CNO (G2), 2 g WCO (G3), 0.5 g CNO plus 1.5 g WCO (G4), 1 g CNO plus 1 g WCO (G5), or 1.5 g CNO plus 0.5 g WCO/kg (G6). Live body weight and feed conversion ratio were significantly higher in the G4 and G5 groups than in the other groups. Superoxide dismutase activity and reduced glutathione concentration were significantly improved in the CNO or WCO diets. Supplemental CNO plus WCO at all tested levels produced the best lysozyme and complement 3 activities. Cecal lactobacilli, coliform, Enterobacteriaceae, and Clostridium spp. populations were lower in the group who received the 1 g CNO + 1 g WCO/kg diet than that in the control group. Dietary supplementation of 1 g CNO + 1 g WCO or 0.5 g CNO + 1.5 g WCO/kg had the potential to improve growth, feed utilization, antioxidant status, and immunity, and reduce cecal pathogenic bacteria in rabbits.

scholarly journals Comparative Clinical and Haematological Investigations in Lactating Cows with Subclinical and Clinical Ketosis

2015 ◽  
Vol 38 (2) ◽  
pp. 159-166 ◽  
Author(s):  
Vania Marutsova ◽  
Rumen Binev ◽  
Plamen Marutsov
Keyword(s):  
Weight Loss ◽  
Body Weight ◽  
Blood Cell ◽  
Body Weight Loss ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Distribution Width ◽  
Subclinical Ketosis ◽  
Lactating Cows

Abstract Ketosis of lactating cows is among the most common metabolic diseases in modern dairy farms. The economic importance of the disease is caused by the reduced milk yield and body weight loss, poor feed conversion, lower conception rates, culling and increased mortality of affected animals. In the present study, a total of 47 high-yielding dairy cows up to 45 days in milk (DIM) are included. All animals were submitted to physical examination wich included checking the rectal body temperature, heart rate, respiratory and rumen contraction rates, and inspection of visible mucous coats. The body condition was scored, and blood β-hydroxybutyrate (BHBA) concentrations were assayed. The cows were divided into 3 groups: first group (control) (n=24) with blood β-hydroxybutyrate level <1.2 mmol/l, second group (n=15) with blood β-hydroxybutyrate between 1.2-2.6 mmol/l (subclinical ketosis) and third group (n=8) with blood β-hydroxybutyrate >2.6 mmol/l (clinical ketosis). Whole blood samples were obtained and analyzed for Red Blood Cell (RBC, 1012/l), Hemoglobin (HGB, g/l), Hematocrit (HCT, %), Mean Corpuscular Volume (MCV, fl), Mean Corpuscular Hemoglobin (MCH, pg), Mean Corpuscular Hemoglobin Concentration (MCHC, g/l), White Blood Cell (WBC, 109/l), Lymphocytes (LYM, 109/l), Monocytes (MON, 109/l), Granulocytes (GRA, 109/l), Red Blood Distribution Width (RDW, %), Red Blood Cell Distribution Width Absolute (RDWa, fl), Platelets (PLT, 109/l) and Mean Platelet Volume (MPV, fl). In this study, deviations in the clinical parameters in the control group and in those with subclinical ketosis were not identified. The cows from the third group (clinical ketosis) exhibited hypotonia, anorexia and body weight loss vs. control group. Hematological analysis showed leukocytosis and lymphocytosis in cows with subclinical ketosis vs. control group. In cows with clinical ketosis WBC counts decreased (leukopenia), while hemoglobin content and hematocrit values are higher vs. control group. Blood BHBA values are higher in both groups of ketotic cows vs. the control group. The other analyzed parameters (RBC, MCH, MCHC, MCV, RDW, RDWa, MON, GRA, PLT and MPV) were close to control values.

scholarly journals Use of Chemical Nano-Selenium as an Antibacterial and Antifungal Agent in Quail Diets and Its Effect on Growth, Carcasses, Antioxidant, Immunity and Caecal Microbes

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3027
Author(s):  
Mahmoud Alagawany ◽  
Shaza Y. A. Qattan ◽  
Youssef A. Attia ◽  
Mohamed T. El-Saadony ◽  
Shaaban S. Elnesr ◽  
...  
Keyword(s):  
Body Weight ◽  
Lipid Profile ◽  
Growth Performance ◽  
Carcass Traits ◽  
Dietary Supplementation ◽  
Control Group ◽  
Total Bacterial Count ◽  
Japanese Quails ◽  
Intestinal Pathogens ◽  
The Impact

Nano-minerals are used to enhance mineral bioavailability, which helps improve animal growth and health. The use of chemical nano-selenium (Che-SeNPs) has lately attracted great scientific interest, mainly due to its potential benefits for poultry. The current study was conducted to investigate the impact of the dietary supplementation of Che-SeNPs on the growth performance, carcass traits, blood constituents, antioxidant status, immunity, and gut microbiota of Japanese quails. A total of one week-old 180 Japanese quails were randomly distributed into four equal groups, and each group consisted of 45 unsexed birds with five replications (nine birds each). The first group was fed a basal diet without supplementation (0 g/kg Che-SeNPs), and the second, third, and fourth groups were fed diets containing 0.2, 0.4, and 0.6 g/kg Che-SeNPs, respectively. The results showed that the dietary supplementation of Che-SeNPs significantly (p < 0.0001) increased body weight, body weight gain, and feed conversion ratio, but decreased feed intake (p < 0.0001) compared to the control group. The highest values of growth performance were recorded in the group fed 0.4 g Che-SeNPs g/kg feed. Che-SeNPs levels did not affect the carcass traits, relative organs (except liver), or blood hematology (except platelet count and hemoglobin level) of quails. Plasma total protein, albumin, aspartate amino transferase (AST), and urea values were not affected by dietary Che-SeNPs, but alanine aminotransferase and lactate dehydrogenase values declined. Globulin and creatinine values were linearly increased with the inclusion of Che-SeNPs (0.4 and 0.6 g/kg) in quail diets compared to the control. The supplementation of Che-SeNPs in quail diets significantly improved (p < 0.05) the plasma lipid profile and activities of antioxidant enzymes compared to the control group. Immunoglobulin G values of Che-SeNPs (0.4 and 0.6 g/kg) were higher (p < 0.05) than those in the control group. The groups fed diets supplemented with Che-SeNPs showed lower (p < 0.0001) total bacterial count, total yeast and molds count, Coliform, Escherichia coli, Enterococcus spp., and Salmonella spp. colonization, and higher (p = 0.0003 and 0.0048) lactic acid bacteria counts than those in the control group. In conclusion, Che-SeNPs supplemented up to 0.4 g/kg can improve the performance, lipid profile, antioxidant indices, and immunity, as well as decrease intestinal pathogens in quails during the fattening period (1–5 weeks of age).

scholarly journals Use of Chemical Nano Selenium as Antibacterial and Antifungal Agent in Quail Diets and its Effect on Growth, Carcasses, Antioxidant, Immunity and Caecal Microbe

2021 ◽  
Author(s):  
Mahmoud Alagawany ◽  
M. T. El-Saadony ◽  
S. S. Elnesr ◽  
M. Farahat ◽  
G. Attia ◽  
...  
Keyword(s):  
Body Weight ◽  
Lipid Profile ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Carcass Traits ◽  
Dietary Supplementation ◽  
Control Group ◽  
Total Bacterial Count ◽  
Intestinal Pathogens ◽  
The Impact

Abstract Nano-minerals are used to enhance mineral bioavailability, which helps improve animal growth and health. The use of chemical nano-selenium (Che-SeNP) has lately attracted great scientific interest, mainly due to its potential benefits for poultry. The current study was conducted to investigate the impact of the dietary supplementation of Che-SeNPs on growth performance, carcass traits, blood constituents, antioxidant status, immunity, and gut microbiota of quails. A total of one week-old 180 Japanese quails were randomlydistributed into four equal groups, and each group consisted of 45 unsexed birds with five replications (nine birds each). The first group was fed a basal diet without supplementation (0 g/kg Che-SeNP), and the second, third, and fourth groups were fed diets containing 0.2, 0.4, and 0.6 g/kg Che-SeNP, respectively. The results showed that the dietary supplementation of Che-SeNP significantly (P < 0.05) increased body weight, body weight gain, and feed conversion ratio but decreased feed intake (P < 0.001) compared to the control group. The highest values of growth performance were recorded in the group fed 0.4 g Che-SeNP g/kg feed. Che-SeNP levels did not affect carcass traits, relative organs (except liver), or blood hematology (except platelet count and hemoglobin level) of quails. Plasma total protein, albumin, aspartate aminotransferase (AST) and urea values were not affected by dietary Che-SeNP, but alanine aminotransferase and lactate dehydrogenase values declined. Globulin and creatinine values were linearly increased with the inclusion of Che-SeNP (0.4 and 0.6 g/kg) in quail diets compared to the control. The supplementation of Che-SeNP in quail diets significantly improved (P < 0.05) the plasma lipid profile and activities of antioxidant enzymes compared to the control group. Immunoglobulin G values of Che-SeNPs (0.4 and 0.6 g/kg) were higher (P < 0.05) than those in the control group. The groups fed diets supplemented with Che-SeNP showed lower (P < 0.0001) total bacterial count, total yeast and molds count, Coliform, Escherichia coli, Enterobacter, and Salmonella colonization, and higher (P = 0.0003 and 0.0048) lactic acid bacteria counts than those in the control group. In conclusion, dietary supplementation with Che-SeNP can improve the performance, lipid profile, antioxidant indices, and immunity, as well as decrease intestinal pathogens in quails.

scholarly journals Effect of Dietary Zinc Supplementation on the Productive Performances, Carcass Traits and Blood Profile of Broiler

2021 ◽  
Vol 9 (10) ◽  
pp. 1938-1944
Author(s):  
Firojul Islam ◽  
Md Ruknuzzaman ◽  
Abdul Gaffar Miah ◽  
Ummay Salma
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Dietary Treatment ◽  
Basal Diet ◽  
Control Group ◽  
Dietary Zinc ◽  
Blood Profile ◽  
Carcass Yield ◽  
Treatment Groups ◽  
Net Profit

This study was conducted to determine the effect of zinc on the productive performances, carcass yield and blood profiles of broilers. Experiment was done for a period of 35 days with a number of 60, day old broiler chicks. Birds were divided into four dietary treatment groups with 3 replications each having 5 birds per replication. The dietary treatment groups were; T0 (basal diet; no zinc), T1 (basal diet + 50 mg zinc/kg feed), T2 (basal diet + 100 mg zinc/kg feed), T3 (basal diet + 150 mg zinc/kg feed). Results revealed that body weight and body weight gain were significantly increased in the zinc supplemented group compared to the control group. Higher feed intake and better FE were also observed in the zinc supplemented group than the control group and the best performance was observed in the group fed 150mg zinc/kg feed (T3). Carcass yield was significantly higher in the groups fed dietary zinc. Net profit was slightly higher in the T3 group but not significantly (P.0.05) differed with the control group. However, T1 and T2 showed less profit even than the control group. Blood profile of the experimental birds including Hb, PCV, and ESR did not significantly differ among the groups. The results indicate that the addition of zinc in the broiler diet improves productive performances, carcass yield and increases net profit without affecting the health status of broiler. So, zinc can be used as an effective and useful micronutrient to improve the performances of broiler.

scholarly journals Effects of dietary supplementation with breadfruit leaf powder on growth performance, meat quality, and antioxidative activity in Japanese quail

2021 ◽  
pp. 1946-1953
Author(s):  
Muhammad Thohawi Elziyad Purnama ◽  
Eric Putra Ernanda ◽  
Faisal Fikri ◽  
Agus Purnomo ◽  
Shafia Khairani ◽  
...  
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Meat Quality ◽  
Japanese Quail ◽  
Antioxidative Activity ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Drip Loss ◽  
Treatment Groups ◽  
Leaf Powder

Background and Aim: In an era of increasing concerns about food availability globally, poultry meat is being increasingly consumed rather than red meat given its quality in terms of pH, color, and tenderness, conferring consumer satisfaction. The choice of feed is a crucial factor in poultry production. This study investigated the effect of dietary supplementation with breadfruit leaf powder on growth performance, meat quality, and antioxidative activity in Japanese quail. Materials and Methods: A total of 120 day-old quail were used in this study and assigned equally into four treatment groups: Group C fed a basal diet and three treatment groups fed a basal diet supplemented with 2.5% (T1), 5% (T2), or 10% (T3) breadfruit leaf powder. The concentrations of breadfruit leaf powder were 2.5, 5, and 10 g/kg in the basal diet. Quail body weight and feed intake (FI) were evaluated at 1, 21, and 35 days of age at 7 a.m. Pectoral muscle was collected to determine pH, meat color, drip loss, cooking loss, water-holding capacity (WHC), tenderness, and antioxidant levels. All variables were analyzed statistically using ANOVA followed by Duncan's post hoc test (significance set at p<0.05). Results: T3 showed increased body weight gain of quails at 1-21 and 21-35 days (p<0.05). Feeding in the T3 group improved the feed conversion ratio compared with those in the C and T1 groups at the starter phase (p<0.05). Dietary treatment did not affect FI (p>0.05). In the present study, meat redness and WHC were improved in the T3 group (p<0.05). Meanwhile, drip loss, cooking loss, and meat tenderness were improved in the T2 group (p<0.05). The pH45 min, pH24 h, lightness, and yellowness were not influenced by the treatments (p>0.05). The antioxidative activities of superoxide dismutase and malondialdehyde decreased in the T3 group (p<0.05), while no significant difference in glutathione peroxidase level (p>0.05) was identified. Conclusion: Ten grams/kilogram of breadfruit leaf powder, as administered in the T3 group, can be applied as a dietary supplement for Japanese quail to improve growth performance, meat quality, and antioxidative activity during the starter and grower periods.

scholarly journals Pengaruh Aflatoksin 9,58 ppb dalam Pakan Terhadap Biometrik Saluran Cerna dan Berat Badan Ayam Petelur Periode Awal Pertumbuhan

2021 ◽  
Vol 9 (2) ◽  
pp. 69
Author(s):  
Dio Putra Pratama ◽  
Benjamin Chr. Tehupuring ◽  
Emmanuel Djoko Poetranto ◽  
Hana Eliyani ◽  
Soeharsono Soeharsono ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Treatment Group ◽  
Digestive Tract ◽  
Growth Period ◽  
Basal Diet ◽  
Early Growth ◽  
Control Group ◽  
Treatment Groups ◽  
First Feeding

The purpose of this study was to determine the effect of aflatoxin content of 9.58 ppb in feed on gastrointestinal biometrics and weight gain of laying hens in the early growth period. Thirty-six day old chicks (DOC) were used in this study and were divided into two groups P0 and P1. P0 (control group) was fed with 100% basal diet and P1 (treatment group) was fed a diet with a composition of 80% basal feed + 20% aflatoxin-containing feed. After the DOC was adapted, the first feeding containing aflatoxin was given from the 20th day to the 60th day. Data collection was carried out three times, namely the 20th, 40th, and 60th days. At the end of each period, the chickens would be euthanized to collect data on the weight and length of the digestive tract and body weight. Weight gain with time increased, but had no significant effect (p>0.05) between the control and treatment groups. Body weight continues to increase because the body's homeresis function is still running well. Observations on gastrointestinal biometrics showed significant differences (p<0.05) in oesophagus weight, proventriculus weight, ventriculus weight, jejunum weight, ileum weight and length, and cecum weight because aflatoxins have a direct effect on the digestive tract they pass. The conclusion of this study was that the provision of 20% aflatoxin-containing feed did not affect weight gain but had shown an effect on the biometrics of several gastrointestinal tracts.

Pro-oxidant effects of a high α-tocopherol dose on kidney antioxidant biomarkers and histopathological aspects

2017 ◽  
Vol 87 (3-4) ◽  
pp. 179-190
Author(s):  
Amel Kanane ◽  
Fayrouz Rouaki ◽  
Mohamed Brahim Errahmani ◽  
Abdenour Laraba ◽  
Hayet Mesbah ◽  
...  
Keyword(s):  
Sunflower Oil ◽  
Antioxidant Status ◽  
Basal Diet ◽  
Inflammatory Cells ◽  
Significant Rise ◽  
Control Group ◽  
Histopathological Changes ◽  
Group 5 ◽  
Oxidant Effect ◽  
Higher Doses

Abstract. The aim of this study is to evaluate the effect of α-tocopherol supplementation at two doses (600 and 1200 mg × kg–1) on kidney antioxidant status and the histopathological changes in Wistar rats after 12 weeks of exposure at different diets. Forty rats has been divided into 4 groups of 10 rats each, the control group received basal diet with 5 % fresh sunflower oil (FSO), the second group: 5 % oxidized sunflower oil (OSO), the third group: 5 % OSO supplemented with 600 mg × kg–1 α-tocopherol and the fourth group: 5 % OSO supplemented with 1200 mg × kg–1 α-tocopherol. In OSO groups, the results showed highly significant increases of LPO (from 31.3 ± 0.9 to 53.8 ± 1.2 nmol of MDA formed/min/mg protein, p < 0.0001) with a significant decrease (p < = 0.001) of the antioxidant enzymatic activities (CAT, SOD, GPX, GR and G6PDH), body weight (339 ± 9 to 290 ± 3 g) and α-tocopherol levels (13.6 ± 0.6 to 6.5 ± 0.4 μg/mg protein). In OSO groups with 600 mg × kg–1 α-tocopherol, an antioxidant effect was found, reflected by a return of the parameters to values similar to those of the control group. However, higher doses of α-tocopherol (1200 mg × kg–1) induced a depletion of antioxidant status, α-tocopherol levels (6.0 ± 0.3 μg/mg protein, p < 0.001) and a very highly significant rise (p < 0.0001) of LPO content (54.86 ± 0.01 nmol of MDA formed/min/mg protein). The kidney tissues also showed changes in glomerular, severe inflammatory cells infiltration, and formation of novel vessels. So, we can conclude that the oxidative stress is attenuated by a moderate administration of 600 mg × kg–1 α-tocopherol, while a pro-oxidant effect occurs at 1200 mg × kg–1 α-tocopherol.

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