Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality, and Risk Factors for Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Goat Herds

The objectives of this work were to study the prevalence and the patterns of antibiotic resistance of staphylococcal isolates from bulk-tank milk of goat herds across Greece, to assess possible associations of the presence of antibiotic resistance with the quality of milk in these herds and to evaluate herd-related factors potentially associated with the presence of antibiotic resistance among these staphylococcal isolates. A cross-sectional study was performed on 119 goat herds in Greece. Bulk-tank milk samples were collected for bacteriological examination; staphylococcal isolates were evaluated for resistance to 20 antibiotics. Oxacillin-resistant, resistant to at least one antibiotic, and multi-resistant staphylococcal isolates were recovered from 5.0%, 30.3%, and 16.0% of herds, respectively. Of 80 isolates, 7.5% were resistant to oxacillin, 50.0% were resistant to at least one antibiotic and 27.5% were multi-resistant. Resistance was seen more frequently among coagulase-negative staphylococci (59.3%) than among Staphylococcus aureus (23.8%). Resistance was more frequent against penicillin and ampicillin (41.3% of isolates) and fosfomycin (27.5%). No association was found with biofilm formation by staphylococci. For recovery of oxacillin-resistant isolates, the presence of working staff in the herds emerged as a significant factor; respective factors for the isolation of staphylococci resistant to at least one antibiotic were part-time farming and high (>10) number of systemic disinfections in the farm annually. The same three factors concurrently were also identified to be significant for the recovery of multi-resistant isolates.

Download Full-text

Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Sheep Flocks: Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality, and Risk Factors

Biology ◽

10.3390/biology10101016 ◽

2021 ◽

Vol 10 (10) ◽

pp. 1016

Author(s):

Daphne T. Lianou ◽

Efthymia Petinaki ◽

Peter J. Cripps ◽

Dimitris A. Gougoulis ◽

Charalambia K. Michael ◽

...

Keyword(s):

Antibiotic Resistance ◽

Biofilm Formation ◽

Early Stage ◽

Cross Sectional Study ◽

Lactation Period ◽

Related Factors ◽

Cross Sectional ◽

Bulk Tank Milk ◽

Bulk Tank ◽

Quality Of Milk

The objectives of this work were to study prevalence and characteristics of resistance to antibiotics of staphylococcal isolates from the bulk-tank milk of sheep flocks across Greece, to assess possible associations of the presence of antibiotic resistance with the quality of milk in these flocks and to evaluate flock-related factors potentially associated with antibiotic resistance among these isolates. A cross-sectional study was performed in 325 sheep flocks in Greece. Bulk-tank milk samples were collected for bacteriological examination; staphylococcal isolates were evaluated for resistance to 20 antibiotics. Oxacillin-resistant staphylococcal isolates, isolates resistant to any antibiotic, and multi-resistant isolates were recovered from 8.0%, 30.5%, and 12.0% of flocks, respectively. Of 232 isolates, 11.6% were resistant to oxacillin, 46.1% were resistant to at least one antibiotic, and 16.4% were multi-resistant. Resistance was seen more frequently among coagulase-negative (50.6%) than among Staphylococcus aureus (31.5%) isolates. Resistance was more frequent against penicillin and ampicillin (34.1% of isolates), clindamycin (17.7%), and fosfomycin (14.2%). An association was found between biofilm formation by staphylococci and resistance to fosfomycin. For recovery of oxacillin-resistant isolates, the lack of experience by farmers emerged as a significant factor; respective factors for the isolation of staphylococci resistant to any antibiotic or multi-resistant isolates were the early stage of the lactation period (0th–1st month) and the intensive management system applied in the flocks, respectively.

Download Full-text

Genetic Determinants of Biofilm Formation and Antibiotic Resistance of Helicobacter Pylori using Whole Genome Sequencing

10.21203/rs.3.rs-800655/v1 ◽

2021 ◽

Author(s):

Kartika Afrida Fauzia ◽

Hafeza Aftab ◽

Muhammad Miftahussurur ◽

Langgeng Agung Waskito ◽

Vo Phuoc Tuan ◽

...

Keyword(s):

Helicobacter Pylori ◽

Antibiotic Resistance ◽

Biofilm Formation ◽

Cross Sectional Study ◽

Whole Genome ◽

Nucleotide Polymorphisms ◽

Genetic Determinants ◽

Genome Sequences ◽

Cross Sectional ◽

H Pylori

Abstract The nucleotide polymorphisms (SNPs) associated with the biofilm formation phenotype of Helicobacter pylori were investigated. Fifty-six H. pylori isolates from Bangladeshi patients were included in this cross-sectional study. Crystal violet was used to classify the phenotypes into high- and low-biofilm formers. Whole genome sequences were analyzed using the “Antimicrobial Resistance Identification By Assembly” (ARIBA) pipeline. The results indicated 19.6% high- and 81.4% low-biofilm formers. These phenotypes were not related to specific clades in the phylogenetic analysis. Biofilm formation was significantly associated with SNPs of alpA, alpB, cagE, cgt, csd4, csd5, futB, gluP, homD, and murF (P < 0.05). Among the SNPs reported in alpB, strains encoding the N156K, G160S, and A223V mutations were high-biofilm formers. Mutations associated with antibiotic resistance can be detected. This study revealed the potential role of SNPs to biofilm formation, and propose a method to detect mutation in antibiotic resistance and biofilm from whole genome sequences.

Download Full-text

BIOFILM FORMATION AND ANTIBIOTIC SUSCEPTIBILITY OF STROKE PATIENT’S URINARY CATHETER ISOLATES AT BETHESDA HOSPITAL, YOGYAKARTA

Berkala Ilmiah Kedokteran Duta Wacana ◽

10.21460/bikdw.v4i2.146 ◽

2019 ◽

Vol 4 (2) ◽

pp. 58

Author(s):

Christofer Sathya Wijaya Budi Sarwono

Keyword(s):

Antibiotic Resistance ◽

Urinary Tract ◽

Antibiotic Susceptibility ◽

Biofilm Formation ◽

Urinary Catheter ◽

Cross Sectional Study ◽

Stroke Patients ◽

Plate Method ◽

Cross Sectional ◽

E Coli

Background: Antibiotic resistance currently challenges infectious disease management, specifically in combating biofilm formation. Biofilm might be developed on urinary catheter, in which stroke patients with urinary tract problem have higher risk of catheter associated urinary tract infection and problems related to antibiotic resistance. Aim: This study describes the microbe types from catheter isolates, measuring the antibiotic susceptibility and biofilm formation, especially in stroke patients at Bethesda Hospital, Yogyakarta. Method: This is a cross-sectional study, describing findings of specimens from stroke patients at Bethesda Hospital, since December 2018 to January 2019. The isolates were identified with standard method, the antibiotic susceptibility were tested with antibiotic disc on MHA medium, and the biofilm formation were assessed using tissue culture plate method. All procedure were done in Microbiology Laboratorium, FK UKDW. Result: Thirty three microbes were isolated from 30 specimens of urinary catheter (63,3% male, 36,3% female). There is 76% isolates incapable of developing biofilm, while 15% is moderate biofilm producer and 9% is strong biofilm producer. The susceptibility test showed 100% resistancy of ampicillin-sulbactam, tetracyclin, ketoconazole, itraconazole, and terbinafine. Around 50% of E. coli and 57% of K. pneumonias isolates is MDR, and 75% of A. baumanii is XDR. Conclusion: Isolated microbes from urinary catheter of stroke patients at Bethesda Hospital is dominated by E. coli, K. pneumoniae, and A. baumanii. Some of the microbes could form strong biofilm, and some of the antibiotics could not be used in the future due to their 100% resistancy on all specimens.

Download Full-text

Cross-sectional study on the prevalence of contagious pathogens in bulk tank milk and their effects on somatic cell counts and milk yield

Italian Journal of Animal Science ◽

10.1080/1828051x.2019.1693282 ◽

2019 ◽

Vol 19 (1) ◽

pp. 66-74

Author(s):

Alfonso Zecconi ◽

Francesca dell’Orco ◽

Nicoletta Rizzi ◽

Diego Vairani ◽

Micaela Cipolla ◽

...

Keyword(s):

Somatic Cell ◽

Milk Yield ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Bulk Tank Milk ◽

Somatic Cell Counts ◽

Cell Counts ◽

Bulk Tank

Download Full-text

Isolation, Identification and Antimicrobial Resistance Profiles of Salmonella from Dairy Farms in Adama and Modjo Towns, Central Ethiopia

European Journal of Medical and Health Sciences ◽

10.34104/ejmhs.02001011 ◽

2020 ◽

pp. 1-11 ◽

Cited By ~ 2

Keyword(s):

Susceptibility Testing ◽

Dairy Farms ◽

Cross Sectional Study ◽

Multi Drug Resistance ◽

Sectional Study ◽

Cross Sectional ◽

Bulk Tank Milk ◽

Bulk Tank ◽

Cattle Farms ◽

Foodborne Infections

A cross-sectional study was carried out from February 2019 to May 2019 in Adama and Modjo aiming at isolating Salmonella from dairy cattle farms and determining the antimicrobial susceptibility testing of the isolates. A total of 117 samples from dairy farms: faces, bulk tank milk, personnel hand swab, and contaminated floor samples were collected and screened for the presence of Salmonella. Ten (8.5%) of the samples tested were found to be positive for Salmonella. Of 89 faces, 10 bulk tank milk, 9 personnel hand swab, and 9 contaminated floor samples, no positive was found in the milker’s hand swab samples from both Adama and Modjo areas and the isolation frequencies of Salmonella were 8.98%, 10%, and 11.1% in faces, bulk tank milk, and floor sample, respectively. The antibiogram testing revealed differential multi-drug resistance among Salmonella isolates in lactating cow and cows environment samples. Most of the isolates were resistant to methicillin, streptomycin, and nalidixic acid whereas sensitivity was recorded for gentamicin. In conclusion, the relatively high resistance among the bacteria present in dairy farms could pose public health and therapeutic problems to consumers as potential vehicles of resistant Salmonella foodborne infections.

Download Full-text

Studying and More: Part-Time Employment as an Educational Challenge?

Journal of Medical Psychology ◽

10.3233/jmp-200024 ◽

2020 ◽

Vol 1 (1) ◽

pp. 57-63

Author(s):

Silke Heuse ◽

Cathrin Dietze ◽

Daniel Fodor ◽

Edgar Voltmer

Keyword(s):

Health Care ◽

Perceived Stress ◽

Health Care Professionals ◽

Cross Sectional Study ◽

Study Data ◽

Future Health ◽

Cross Sectional ◽

Professional Students ◽

Educational Challenge ◽

Part Time

Background: Future health-care professionals face stress both during education and in later professional life. Next to educational trainings, many students are forced to assume part-time employment. Objective: Applying the Job Demands-Resources Model to the educational context, we investigate which role part-time employment plays next to health-care professional students’ education-specific demands and resources in the prediction of perceived stress. Method: In this cross-sectional study, data from N = 161 health-care students were analysed, testing moderation models. Results: Education-specific demands were associated with higher and education-specific resources with lower amounts of perceived stress. Part-time employment functioned as moderator, i.e. demands were less associated with stress experiences in students who were employed part-time. Conclusion: Identifying part-time employment as a resource rather than a demand illustrates the need to understand students’ individual influences on stress. Both educators and students will benefit from reflecting these resources to support students’ stress management.

Download Full-text

FOOD CONSUMPTION OF CHILDREN AGED 1 TO 5 IN PHUOC VINH WARD, HUE CITY BY 24 HOURS FOOD RECORDING

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2015.4_5.21 ◽

2015 ◽

pp. 153-161

Author(s):

Thi Bach Yen Hoang ◽

Thi Hai Pham ◽

Dinh Tuyen Hoang ◽

Thi Huong Le ◽

Van Thang Vo

Keyword(s):

Total Energy ◽

Food Consumption ◽

Economic Status ◽

Cross Sectional Study ◽

Food Groups ◽

Food Record ◽

Related Factors ◽

Cross Sectional ◽

Food Consumption Survey ◽

Food Consumed

Food consumption survey is an essential parts of nutrition surveys. It helps to determine the type and quantity of food consumed, assessing the balance of the diet, the relationship between nutrient intake and health, diseases, and economic status, culture society... There are many methods to investigate food consumption. 24-hour food record is a method that record all food consumed by the subject during previous 24 hours. Using this method in chidren helps to assess the their diet to see if it responses the demand in order to have proper nutrition. Objectives: 1. Calculating the number of each food groups consumed within 24 hours of children 1 to 5 years in Phuoc Vinh ward, Hue City; 2. Assessing the quality of their diet and some related factors. Methodology: A cross-sectional study was implemented on 200 pairs of children aged 1 to 5 and parents or caregivers living in Phuoc Vinh ward, Hue city and some related factors. Results: 82% of the children’s diets covered 4 food groups. Prevalence of glucide, protein, lipide out of the total energy intake were 44.1%, 19.5%, 36.3% respectively within group of 12-<48 months and 50%, 19.5%, 30.6% respectively within group of 48-<72 months. Total energy and protein intake were higher than demanded (p <0.05) while glucide and lipide were lower than demanded (p <0.05). Economical status of family was significant associated with variety of food (all 4 food groups) in the diet of children (p <0.05) and total energy consumed (p <0.05). Conclusion: The children did not have proper nutrition so further research need to be implemented to have suitable interventions. Key words: 24 hours food records, children aged 1 to 5, Hue city.

Download Full-text

HIGH PREVALENCE OF MALNUTRITON AND WORM INFECTION AMONG VAN KIEU AND PAKOH CHILDREN AGED 12-36 MONTHS IN DAKRONG DISTRICT, QUANG TRI PROVINCE

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2012.5.16 ◽

2012 ◽

pp. 129-134

Author(s):

Thi Lan Tran ◽

Thi Huong Le ◽

Xuan Ninh Nguyen

Keyword(s):

Cross Sectional Study ◽

Age Group ◽

Infection Status ◽

Sectional Study ◽

Group Recommendation ◽

Related Factors ◽

Normal Children ◽

Cross Sectional ◽

High Prevalence ◽

Very High

Objectives: Assess the nutritional status, worm infection status and some related factors among children aged 12-36 months of Dakrong district, Quang Tri province. Subject and method: A cross sectional study was carried out in 2010, in 680 children aged 12-36 months in 4 communes of Dakrong district, Quang Tri province. Results: The malnutrition rate was 55.0% for underweight, 66.5% for stunting and 16.2% for wasting. The prevalence of malnutrition increases by age group. The prevalence of worm infection was 31.6%, the highest prevalence was belong to Ascaris infection (24.6%), followed by Hookworm and Trichuris (6.5% and 6.2%, respectively). The prevalence of worm infection among children under two is very high (27.0%). The prevalence of worm infection was distributed quite equally between the malnutrition children group and normal children group. Recommendation: Early deworming forchildren from 12 months should be considered as important strategy against the malnutrition of children in Dakrong district, Quang Tri province

Download Full-text

Association between procrastination, white-collar work and obesity in Japanese male workers: a cross-sectional study

BMJ Open ◽

10.1136/bmjopen-2019-029931 ◽

2019 ◽

Vol 9 (11) ◽

pp. e029931 ◽

Cited By ~ 1

Author(s):

Akihiko Narisada ◽

Kohta Suzuki

Keyword(s):

Weight Gain ◽

White Collar ◽

Cross Sectional Study ◽

Blue Collar ◽

Sectional Study ◽

Related Factors ◽

Cross Sectional ◽

Adult Weight ◽

Japanese Male ◽

Male Workers

ObjectiveTo investigate the associations among procrastination (time inconsistency), work environment and obesity-related factors in Japanese male workers.DesignCross-sectional study.SettingData were collected at two work sites of Japanese electronics manufacturing company in 2015.Participants795 full-time male workers in a Japanese electric company, aged 35–64 years, who underwent health checkups in 2015.Main outcome measuresBody mass index (BMI), adult weight change, obesity (BMI ≥25 kg/m2), adult weight gain over 10 kg (AWG10) and metabolic syndrome (MetS). Multivariable linear and logistic regression analyses were performed to assess the associations of procrastination assessed by using a one-item questionnaire and white-collar and blue-collar work with obesity-related factors.ResultsWhite-collar workers with high procrastination levels showed positive associations with BMI (B: 0.75, 95% CI 0.06 to 1.44) and adult weight change (B: 1.77, 95% CI 0.26 to 3.29), and had increased odds of AWG10 (OR: 1.85, 95% CI 1.04 to 3.29) and MetS (OR: 2.29 95% CI 1.18 to 4.44) after adjustment for age, education, work-related factors and lifestyle factors. However, such positive associations were not observed among blue-collar workers.ConclusionsProcrastination and white-collar work might have a joint effect on weight gain during adulthood and consequential obesity.

Download Full-text