scholarly journals Identifying a Role of Red and White Wine Extracts in Counteracting Skin Aging: Effects of Antioxidants on Fibroblast Behavior

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 227
Author(s):  
Sara Cruciani ◽  
Margherita Trenta ◽  
Giovanna Rassu ◽  
Giuseppe Garroni ◽  
Giacomo Luigi Petretto ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Raw Materials ◽  
Colorimetric Assay ◽  
White Wine ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Stressful Event ◽  
Aging Effects ◽  
Skin Function ◽  
Cellular Components

Dermal fibroblasts are the main actor in many proteins’ secretion, including collagen, preserving skin function. Free radicals are involved in skin aging and damages involving different cellular components. The imbalance between reactive oxygen species (ROS) amount and natural antioxidant enzymes negatively affects skin homeostasis. Natural compounds have recently emerged as a potential anti-aging tool in tissue regeneration. In the present paper we evaluated the antioxidant activity of white and red wines, considering their probable use, as raw materials, for the formulation of cosmetic products with anti-aging properties. We studied a method that would allow the removal of the alcoholic fraction of wines and determined their composition by LC-MS analysis. We then tested the possible cytotoxic effects of red and white wines on fibroblasts by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay, and their antioxidant activity by the catalase activity test in stressing conditions. Finally, we evaluated their anti-aging potential through the β-galactosidase colorimetric assay. Our results showed that wine extracts exhibit a remarkable antioxidant and anti-aging activity, especially on cells exposed to a marked stressful event. These properties could suggest their possible application as cosmetical products for skin regeneration.

scholarly journals Synthesis of Kisspeptin-Mimicking Fragments and Investigation of their Skin Anti-Aging Effects

2020 ◽  
Vol 21 (22) ◽  
pp. 8439
Author(s):  
Kyung-Eun Lee ◽  
Sugyeong Jeong ◽  
Seok Kyun Yun ◽  
Seoyeon Kyung ◽  
Abadie Sophie ◽  
...  
Keyword(s):  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Biologically Active ◽  
Type I ◽  
Aging Effects ◽  
Type I Procollagen ◽  
Reproductive Axis ◽  
Stress Related Genes ◽  
Active Fragments ◽  
Aging Models

In recent years, a number of active materials have been developed to provide anti-aging benefits for skin and, among them, peptides have been considered the most promising candidate due to their remarkable and long-lasting anti-wrinkle activity. Recent studies have begun to elucidate the relationship between the secretion of emotion-related hormones and skin aging. Kisspeptin, a neuropeptide encoded by the KISS1 gene, has gained attention in reproductive endocrinology since it stimulates the reproductive axis in the hypothalamus; however, the effects of Kisspeptin on skin have not been studied yet. In this study, we synthesized Kisspeptin-10 and Kisspeptin-E, which are biologically active fragments, to mimic the action of Kisspeptin. Next, we demonstrated the anti-aging effects of the Kisspeptin-mimicking fragments using UV-induced skin aging models, such as UV-induced human dermal fibroblasts (Hs68) and human skin explants. Kisspeptin-E suppressed UV-induced 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) stimulation leading to a regulation of skin aging related genes, including type I procollagen, matrix metalloproteinases-1 (MMP-1), interleukin-6 (IL-6), and IL-8, and rescued the skin integrity. Taken together, these results suggest that Kisspeptin-E could be useful to improve UV-induced skin aging by modulating expression of stress related genes, such as 11β-HSD1.

scholarly journals Propolis Suppresses UV-Induced Photoaging in Human Skin through Directly Targeting Phosphoinositide 3-Kinase

Nutrients ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3790
Author(s):  
Da Hyun Kim ◽  
Joong-Hyuck Auh ◽  
Jeongyeon Oh ◽  
Seungpyo Hong ◽  
Sungbin Choi ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Human Skin ◽  
Therapeutic Potential ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Kinase Assay ◽  
Aging Effects ◽  
Propolis Extract ◽  
Pi3k Activity ◽  
Phosphoinositide 3 Kinase

Propolis is a resinous substance generated by bees using materials from various plant sources. It has been known to exhibit diverse bioactivities including anti-oxidative, anti-microbial, anti-inflammatory, and anti-cancer effects. However, the direct molecular target of propolis and its therapeutic potential against skin aging in humans is not fully understood. Herein, we investigated the effect of propolis on ultraviolet (UV)-mediated skin aging and its underlying molecular mechanism. Propolis suppressed UV-induced matrix metalloproteinase (MMP)-1 production in human dermal fibroblasts. More importantly, propolis treatment reduced UV-induced MMP-1 expression and blocked collagen degradation in human skin tissues, suggesting that the anti-skin-aging activity of propolis can be recapitulated in clinically relevant conditions. While propolis treatment did not display any noticeable effects against extracellular signal-regulated kinase (ERK), p38, and c-jun N-terminal kinase (JNK) pathways, propolis exerted significant inhibitory activity specifically against phosphorylations of phosphoinositide-dependent protein kinase-1 (PDK1) and protein kinase B (Akt). Kinase assay results demonstrated that propolis can directly suppress phosphoinositide 3-kinase (PI3K) activity, with preferential selectivity towards PI3K with p110α and p110δ catalytic subunits over other kinases. The content of active compounds was quantified, and among the compounds identified from the propolis extract, caffeic acid phenethyl ester, quercetin, and apigenin were shown to attenuate PI3K activity. These results demonstrate that propolis shows anti-skin-aging effects through direct inhibition of PI3K activity.

scholarly journals Rapamycin Protects Skin Fibroblasts From UVA-Induced Photoaging by Inhibition of p53 and Phosphorylated HSP27

2021 ◽  
Vol 9 ◽  
Author(s):  
Gen-Long Bai ◽  
Ping Wang ◽  
Xin Huang ◽  
Zi-Yue Wang ◽  
Di Cao ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Type I ◽  
Aging Effects ◽  
Smad Signaling Pathway ◽  
Skin Photoaging ◽  
Dermal Collagen ◽  
Induced Apoptosis

Skin aging caused by UV radiation is called photoaging is characterized by skin roughness and dryness accompanied by a significant reduction of dermal collagen. Rapamycin is a macrolide immunosuppressant which has been shown to exhibit “anti-aging” effects in cells and organisms, however, its roles in the skin photoaging remains unclear. Here, we investigate the role of rapamycin and HSP27, which we have previously identified as an inhibitor of UV-induced apoptosis and senescence in HaCat cells, in a UVA-induced photoaging model of primary human dermal fibroblasts (HDFs). Results from senescence-associated beta-galactosidase (SA-β-gal) staining revealed that rapamycin significantly reduced senescence in UVA-treated HDFs. In addition, treatment with rapamycin significantly increased cell autophagy levels, decreased the expression of p53 and phosphorylated HSP27, and reduced genotoxic and oxidative cellular stress levels in UVA-induced HDFs. Knockdown of HSP27 resulted in a significant increase of MMP-1 and MMP-3 as well as a decrease in type I collagen expression. Rapamycin mitigated these effects by activation of the classical TGF-β/Smad signaling pathway and increasing the transcriptional activity of MAPK/AP-1. Taken together, these results suggest that rapamycin may potentially serve as a preventive and therapeutic agent for UVA-induced photoaging of the skin.

scholarly journals Anti-oxidant and Anti-skin-aging Effects of Abalone Viscera Extracts in Human Dermal Fibroblasts

2012 ◽  
Vol 19 (4) ◽  
pp. 463-469 ◽  
Author(s):  
Jinglei Li ◽  
Tao Tong ◽  
Du-Ock Ko ◽  
Dong-Ok Chung ◽  
Won-Chul Jeong ◽  
...  
Keyword(s):  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Aging Effects ◽  
Anti Oxidant

scholarly journals Aqueous Extracts of Mushrooms Enriched with Ergothionein with Antioxidant Activity and Its Effect on Human Dermal Fibroblasts UV irradiated

2021 ◽  
Author(s):  
Anabell Del Rocío Urbina-Salazar ◽  
Alberto Renato Inca-Torres ◽  
Bryan Anthony Urbina-Salazar ◽  
Valeria Fernanda Inca-Torres ◽  
Juan Bautista
Keyword(s):  
Antioxidant Activity ◽  
Agaricus Bisporus ◽  
Cellular Response ◽  
Enzymatic Digestion ◽  
Dermal Fibroblasts ◽  
Aqueous Extracts ◽  
Aging Effects ◽  
Matrix Metalloproteinase 1 ◽  
Tnf Α ◽  
Potential Applications

Ergotioneine (ERG) has potential applications as food additive and as an antioxidant for the treatment and/or prevention of diseases related to oxidative stress. In this work, we study the nature of antioxidant activity and investigate the effect of aqueous extracts of Agaricus bisporus enriched with ergotioneine (EAHE-ERG), a natural product, obtained through enzymatic digestion and membrane technology, in cellular response induced by UV, in crops fibroblasts. EAHE-ERG suppressed by the increase of TNF-α by UV-B radiation. Furthermore, in fibroblasts exposed to UV-A, EAHE-ERG suppressed the expression of matrix metalloproteinase-1 protein (MMP-1) in almost 50% and reduced the RNAm expression from MMP-1. From these results, we conclude that EAHE-ERG can reduce anti-aging effects of the skin from UV radiation by removing O2 and 1O2, and the reduction protease signs and inflammatory activity. Keywords: Ergotioneine, Agaricus bisporus, Antioxidant, Nutraceutical, Anti-inflammatory. Resumen La ergotioneina (ERG) tiene aplicaciones potenciales como un aditivo alimentario y como un antioxidante para el tratamiento y/o prevención de enfermedades relacionadas con el estrés oxidativo. En este trabajo estudiamos la naturaleza de la actividad antioxidante e investigamos el efecto de los extractos acuosos de Agaricus bisporus enriquecidos con ergotioneina (EAHEERG), un producto natural, obtenido mediante digestión enzimática y tecnología de membrana, en respuesta celular inducida por UV, en fibroblastos cultivados. EAHE-ERG suprimió la regulación por incremento de TNF-α mediante radiación UV-B. Además, en fibroblastos expuestos a UV-A, EAHE-ERG suprimió la expresión de proteína de metaloproteinasa-1 de matriz (MMP-1) en casi 50% y redujo la expresión de ARNm de MMP-1. A partir de estos resultados, concluimos que EAHE-ERG puede reducir los efectos antienvejecimiento de la piel después de la irradiación UV mediante la eliminación de O2 y 1O2, y la reducción de las señales de proteasa y actividad inflamatoria. Palabras Clave: Ergotioneina, Agaricus bisporus, Antioxidante, Nutracéutico, Antiinflamatorio.

scholarly journals The Role of microRNAs in Organismal and Skin Aging

2020 ◽  
Vol 21 (15) ◽  
pp. 5281
Author(s):  
Marta Gerasymchuk ◽  
Viktoriia Cherkasova ◽  
Olga Kovalchuk ◽  
Igor Kovalchuk
Keyword(s):  
Replicative Senescence ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Telomere Maintenance ◽  
Sirtuin 1 ◽  
Aging Effects ◽  
Inhibition Of Proliferation ◽  
Biomarkers Of Aging ◽  
Age Related

The aging process starts directly after birth and lasts for the entire lifespan; it manifests itself with a decline in an organism’s ability to adapt and is linked to the development of age-related diseases that eventually lead to premature death. This review aims to explore how microRNAs (miRNAs) are involved in skin functioning and aging. Recent evidence has suggested that miRNAs regulate all aspects of cutaneous biogenesis, functionality, and aging. It has been noted that some miRNAs were down-regulated in long-lived individuals, such as let-7, miR-17, and miR-34 (known as longevity-related miRNAs). They are conserved in humans and presumably promote lifespan prolongation; conversely, they are up-regulated in age-related diseases, like cancers. The analysis of the age-associated cutaneous miRNAs revealed the increased expression of miR-130, miR-138, and miR-181a/b in keratinocytes during replicative senescence. These miRNAs affected cell proliferation pathways via targeting the p63 and Sirtuin 1 mRNAs. Notably, miR-181a was also implicated in skin immunosenescence, represented by the Langerhans cells. Dermal fibroblasts also expressed increased the levels of the biomarkers of aging that affect telomere maintenance and all phases of the cellular life cycle, such as let-7, miR-23a-3p, 34a-5p, miR-125a, miR-181a-5p, and miR-221/222-3p. Among them, the miR-34 family, stimulated by ultraviolet B irradiation, deteriorates collagen in the extracellular matrix due to the activation of the matrix metalloproteinases and thereby potentiates wrinkle formation. In addition to the pro-aging effects of miRNAs, the plausible antiaging activity of miR-146a that antagonized the UVA-induced inhibition of proliferation and suppressed aging-related genes (e.g., p21WAF-1, p16, and p53) through targeting Smad4 has also been noticed. Nevertheless, the role of miRNAs in skin aging is still not fully elucidated and needs to be further discovered and explained.

scholarly journals Enzyme-Treated Asparagus Extract Attenuates Hydrogen Peroxide-Induced Matrix Metalloproteinase-9 Expression in Murine Skin Fibroblast L929 Cells

2016 ◽  
Vol 11 (5) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Ken Shirato ◽  
Jun Takanari ◽  
Junetsu Ogasawara ◽  
Takuya Sakurai ◽  
Kazuhiko Imaizumi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Matrix Metalloproteinase ◽  
Stress Responses ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Activator Protein 1 ◽  
Aging Effects ◽  
Simultaneous Treatment ◽  
Oxidative Stress Responses

Enzyme-treated asparagus extract (ETAS) exerts a wide variety of beneficial biological actions including facilitating anti-cortisol stress and neurological anti-aging responses. However, the anti-skin aging effects of ETAS remain to be elucidated. Reactive oxygen species (ROS) play pivotal roles in skin aging. Increased ROS levels in fibroblasts in response to ultraviolet irradiation activate c-Jun N-terminal kinase (JNK) and its downstream transcription factor activator protein-1 (AP-1), and the resultant gene expression of matrix metalloproteinase (MMP) isoforms accelerates collagen breakdown in the dermis. Therefore, we explored whether ETAS has anti-skin aging effects by attenuating the oxidative stress responses in fibroblasts. Simultaneous treatment of murine skin L929 fibroblasts with hydrogen peroxide (H2O2) and either ETAS or dextrin showed that ETAS significantly suppressed H2O2-induced expression of MMP-9 mRNA as measured by real-time polymerase chain reaction. ETAS also clearly suppressed H2O2-stimulated phosphorylation of c-Jun (AP-1 subunit) and JNK as determined by Western blot. However, ETAS did not affect the increased amounts of carbonyl proteins in response to H2O2, also as determined by Western blotting. These results suggest that ETAS diminishes cellular responsiveness to ROS but does not scavenge ROS. Thus, ETAS has the potential to prevent skin aging through attenuating the oxidative stress responses in dermal fibroblasts.

scholarly journals Autophagy in Human Skin Fibroblasts: Impact of Age

2018 ◽  
Vol 19 (8) ◽  
pp. 2254 ◽  
Author(s):  
Hei Kim ◽  
Seo-Yeon Park ◽  
Seok Moon ◽  
Jeong Lee ◽  
Sungjoo Kim
Keyword(s):  
Human Skin ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Skin Fibroblasts ◽  
Autophagic Flux ◽  
Human Skin Fibroblasts ◽  
Waste Production ◽  
Lysosomal Proteolysis ◽  
Autophagic Activity ◽  
Cellular Components

Autophagy is an intracellular stress response that is enhanced under starvation conditions, and also when the cellular components are damaged. Aging accompanies an increase in intracellular stress and has significant impact on the skin. Since dermal fibroblasts are a powerful indicator of skin aging, we compared the autophagic activity of human skin fibroblasts between the young and old. According to TEM analyses, the number of autophagosomes per 1 μm2 cytoplasmic area was similar between young and aged fibroblasts. The amount of LC3 (microtubule-associated protein 1 light chain 3)-II, a form associated with autophagic vacuolar membranes, was also similar between the groups from Western blot analysis. Although residual bodies were more common in aged dermal fibroblasts, LC3 turnover and p62 assay showed little difference in the rate of lysosomal proteolysis between the young and old. RNA-seq analysis revealed that the major autophagy-modulating genes (BECN1, MAP1LC3B, ATG5, ATG7, ULK1, PIK3C3, mTOR) were not differentially expressed with age. Our results suggest that the basal autophagic flux in aged dermal fibroblasts is largely comparable to that of young fibroblasts. However, with a higher speed and amount of waste production in aged cells, we postulate that such autophagic flux may not be sufficient in keeping the old cells “clean”, resulting in skin aging. Aging is a complex process and, as such, the relationship between autophagy and aging is not straightforward. That is to say, autophagy does not simply decline with age. Regardless of the controversies on autophagic activity with age, autophagy plays a crucial role in counteracting aging, and strategies aimed at its modulation should hold promise for the prevention of skin aging.

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