A Novel Combination of γ-Tocopherol-Rich Mixture of Tocopherols and Ascorbic Acid Restores Fertility in Cases of Tyrosine Nitration-Associated Male Infertility in Mice

Infertility is an important health problem that affects up to 16% of couples worldwide. Male infertility is responsible for 50% of the cases. Currently, a physical examination, hormone profiling and the evaluation of two consecutive semen samples (to determine the sperm concentration, motility, morphology and, in very few cases, sperm DNA integrity) are the sole tools that physicians have to evaluate infertility in men. Antioxidant therapy is often used to improve sperm quality and function in infertile men. However, there are controversial results regarding the efficacy of these treatments. Prdx6−/− male mice are subfertile, displaying significant oxidative damage in the lipids, proteins and DNA of their spermatozoa. Here, we used Prdx6−/− male mice to test whether a novel combination of tocopherols that contained 60% γ-tocopherol and ascorbic acid could restore their fertility. These mice were fed with the supplemented (Vit. Mix) or control diets. To assess sperm quality, we determined the motility, levels of lipid peroxidation, DNA oxidation and tyrosine nitration in the spermatozoa. The number of pups sired by the Prdx6−/− mice fed with the Vit. Mix diet was higher than that sired by the males fed with the control diet, and the pups’ mortality was lower. The sperm quality was improved in the males fed with the supplemented diet. We concluded that treatment with a supplement composed of tocopherols and rich in γ-tocopherol and ascorbic acid is effective in restoring fertility in cases where oxidative stress and high levels of tyrosine nitration are associated with male infertility.

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The Impact of Radiofrequency Waves on Male Infertility: A Systematic Review

Shiraz E-Medical Journal ◽

10.5812/semj.101741 ◽

2020 ◽

Vol In Press (In Press) ◽

Author(s):

Leili Darvish ◽

Azadeh Amraee ◽

Marjan Akhavan Amjadi ◽

Zahra Atarodi Kashani ◽

Masoumeh Ghazanfarpour ◽

...

Keyword(s):

Systematic Review ◽

Male Infertility ◽

Sperm Quality ◽

Sperm Count ◽

Sperm Concentration ◽

Membrane Integrity ◽

Semen Parameters ◽

Cochrane Library ◽

Negative Effects ◽

The Impact

Context: As the use of cellphones and other electronic devices increases, concerns about the possible effect of radiofrequency waves on health are growing. Long-term use of the cellphone may have negative effects on sperm quality. Objectives: The purpose of this research was to examine men's infertility due to the effect of radiofrequency waves. Methods: In this systematic review, language restrictions were not considered in searching the databases. Cochrane Library, Google Scholar, PubMed, EMBASE, ProQuest, CINAHL, Science Direct, MEDLINE, and Scopus were used to obtain the data from them. All data were scanned from the year 2000 until 2019. Papers selected for retrieval were evaluated by the Newcastle-Ottawa and CONSORT scales. Results: A total of 14 articles that met the inclusion criteria were ultimately assessed. Motile sperm, sperm vitality and membrane integrity, morphology, volume, total sperm count, sperm concentration, and sperm fertility were found to be influenced by radiofrequency waves. Conclusions: The results showed that RF has detrimental effects on semen parameters and due to an increase in RF wave use currently and its role in male infertility, giving information to men about adverse complications of RF is necessary. Further studies are needed to design the less harmful devices.

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Spermatic multinucleation in infertile agriculture workers exposed to carbofuranyl pesticide

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100166348 ◽

1996 ◽

Vol 54 ◽

pp. 776-777

Author(s):

G. Gallegos de L. ◽

M.M. Arizpe S. ◽

L.E. Alvarado C. ◽

E. Ramirez B.

Keyword(s):

Male Infertility ◽

Causal Relationship ◽

Sperm Motility ◽

Detrimental Effect ◽

Sperm Quality ◽

Semen Analysis ◽

Chemical Agents ◽

And Function ◽

Infertile Patients

It is known that the exposure to gonadotoxic agents cause male infertility in agricutural workers. Pesticides provoke a detrimental effect on the spermatogenesis characterized by low count of sprematozoa, as well as sperm motility and morphology alterations. Simultaneosly an increase in FSH seric levels is present.The semen analysis was proposed in 1975 to detect sperm quality and function alteration induced by chemical agents. There are many morphologic anomalies related to the noxious action of some gonadotoxic agents, ie. head deformities, microcephalia, tail defects, etc.We detected in our laboratory a pair of cases of infertile patients, that presented high levels of teratozoospermia with a high percentage of multinucleated spermatozoa and spermatids, and referred exposure to Carbofuranyl (Furadan 300). Since this agent has not been classified as gonadotoxic, we tried to show it's posible causal relationship with spermatic multinucleation phenomenon observed and the fertility of these patients.

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Review on the role of antioxidant supplementation against oxidative stress: a human and animal approach to male fertility

Research Society and Development ◽

10.33448/rsd-v11i1.25191 ◽

2022 ◽

Vol 11 (1) ◽

pp. e43211125191

Author(s):

Luana Nayara Gallego Adami ◽

Valter Luiz Maciel Junior ◽

João Diego Losano

Keyword(s):

Oxidative Stress ◽

Male Infertility ◽

Male Fertility ◽

Sperm Quality ◽

Human Sperm ◽

Original Data ◽

Experimental Models ◽

Antioxidant Supplementation ◽

And Function

Male infertility is one important factor among the multifactorial causes of couple infertility, being oxidative stress one of the main related sources. Sperm is a specialized cell extremely susceptible to stress. To understand and mitigate this event, many studies have used different antioxidants, orally or in vitro supplementation, trying to improve sperm quality and function. Considering the extensive available literature regarding approaches and attempts to solve male fertility issues, the aim of this review is evaluating the effects of antioxidant supplementation on sperm, in both humans and experimental models with animals. This review selected original data from PubMed. The keywords used were: antioxidant, sperm, male fertility, antioxidant supplementation, male infertility; and the term "rodents" was added to the descriptors “antioxidant” and “male fertility”. Only studies published in indexed journals, in English, between 2015 and 2019 were included. This review involves i) human sperm and ii) rodent sperm. For the human approach, the search retrieved 496 articles and 80 were included, among which 28 studies were of in vitro antioxidant supplementation, 19 involved oral antioxidant supplementation and the remaining 33 concerned quantification of oxidants and antioxidants already present in the seminal samples. For the rodent approach, 152 articles were retrieved and 52 were included: 3 of varicocele, 11 of diabetes, 10 of therapeutic drugs, 3 of physical exercise, 10 of environmental exposure and 3 of heat stress. The remaining studies involved oxidative stress status in experimental models. Antioxidants use for reproductive purposes is increasing in an attempt to achieve better gametes and embryos. Vitamins C, B and E, selenium and zinc are the most commonly used antioxidants, with remarkable evidences in improving pathophysiological seminal conditions.

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Effect of bacterial infection on sperm quality and DNA fragmentation in subfertile men with Leukocytospermia

BMC Molecular and Cell Biology ◽

10.1186/s12860-021-00380-8 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Fatemeh Eini ◽

Maryam Azizi Kutenaei ◽

Fayegheh Zareei ◽

Zeinolabedin Sharifian Dastjerdi ◽

Maryam Hosseinzadeh Shirzeyli ◽

...

Keyword(s):

Bacterial Infection ◽

Dna Fragmentation ◽

Bacterial Infections ◽

Pathogenic Bacteria ◽

Seminal Fluid ◽

Sperm Quality ◽

Sperm Concentration ◽

Dna Integrity ◽

Sperm Dna Fragmentation ◽

Sperm Dna

Abstract Background Although bacterial infections have been recognized as a possible cause of male infertility, the effect of bacterial infections on sperm quality and sperm DNA fragmentation remains controversial. The current study aimed to investigate the prevalence rate of bacterial infection in subfertile men and its effect on semen quality. Seminal fluid was collected from 172 male members of infertile couples attending the andrology infertility center and a group of 35 fertile subjects as a control. Sperm parameters and DNA fragmentation were evaluated based on the type of bacteria in all ejaculates. Results From the 172 patients investigated for infertility, 60 (34.88%) patients had a positive culture for pathogenic bacteria of different species. Leukocytospermia was significantly higher in infected samples in comparison with non-infected samples (p < 0.05). Sperm concentration and motility and morphology were significantly lower in infected than non-infected samples. Moreover, sperm DNA fragmentation was significantly higher in infected than non-infected samples. Besides, our results showed that sperm DNA fragmentation was correlated significantly with leukocytospermia (R: 0.22, p < 0.01). Conclusion The present study suggested that bacterial infection significantly correlated with leukocytospermia could impair male fertility potential through decreasing sperm motility, morphology, and DNA integrity.

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Impact of maternal hyperlipidic hypercholesterolaemic diet on male reproductive organs and testosterone concentration in rabbits

Journal of Developmental Origins of Health and Disease ◽

10.1017/s2040174414000087 ◽

2014 ◽

Vol 5 (3) ◽

pp. 183-188 ◽

Cited By ~ 6

Author(s):

C. Dupont ◽

D. Ralliard-Rousseau ◽

A. Tarrade ◽

C. Faure ◽

M. Dahirel ◽

...

Keyword(s):

Metabolic Diseases ◽

Control Diet ◽

Sperm Concentration ◽

Free Testosterone ◽

Reproductive Organs ◽

Dna Integrity ◽

Membrane Composition ◽

Foetal Development ◽

Male Reproductive Organs ◽

The Impact

The concept of Developmental Origins of Health and Disease initially stemmed from the developmental programming of metabolic diseases. Reproductive functions and fertility in adulthood may also be programmed during foetal development. We studied the impact of dietary-induced maternal hyperlipidaemia and hypercholesterolaemia (HH), administered at 10 weeks of age and throughout the gestation and lactation, on male reproductive functions of rabbit offspring. Male rabbits born to HH dams and fed a control diet had significantly lighter testes and epididymes compared with rabbits born to control dams at adulthood. No significant changes in sperm concentration, sperm DNA integrity and sperm membrane composition were observed, but serum-free testosterone concentrations were decreased in HH males. This study confirms the importance of maternal metabolic status for the development of male reproductive organs.

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Impact of Cigarette Smoking on Sperm Parameters of Infertile Men in Center of Algiers (Capital of Algeria)

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i4-s.4331 ◽

2020 ◽

Vol 10 (4-s) ◽

pp. 193-196

Author(s):

Amina Itatahine ◽

Abbassia Demmouche ◽

H Maï ◽

Z.C. Khalloua ◽

D. Ferrag ◽

...

Keyword(s):

Cigarette Smoking ◽

Male Infertility ◽

Sperm Count ◽

Sperm Concentration ◽

Sperm Parameters ◽

Semen Parameters ◽

Negative Effects ◽

Negative Effect ◽

Pass Through ◽

And Function

Background : Cigarette smoking has negative effects on male fertility. Toxins from tobacco smoking can potentially affect sperm development and function, with a negative effect on semen parameters. In fact, the components of cigarette smoke pass through the blood-testis barrier and thus induce an alteration of sperm parameters and nucleus quality of the spermatozoa. The aim is to determine a diffence between tobbaco and no tobbaco patient and semen parameters. This article aimed to assess the relationship between smoking and male infertility. Subject and Methods : this is retrospective study focused on the effects of smoking on semen analyses. 377 men (165 non-smokers, 212 smokers) with infertility for at least 1 year were evaluated between Mars and November 2018, in center of algiers (capital of Algeria). Sperm characteristics (concentration, motility, morphology, and volume) were detemined. Results: Sperm concentration, percentage motility and morphology were significantly lower in an infertile Smokers group than the infertile Nonsmokers group. We also observed that the infertility duration were significantly increased (p<0.05) in accordance with the age in an infertile Smokers group. Our study shows that cigarette smoking is associated with reduced sperm count and motility. Conclusions: Our results suggest that cigarette smoking has an overall negative effect on semen parameters. Our study suggests that men should be advised to abstain from smoking in order to improve reproductive outcomes. Keywords: smoking, semen, male infertility, sperm, humans

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Spermatogenic Activity and Sperm Traits in Post-Pubertal and Adult Tomcats (Felis catus): Implication of Intra-Male Variation in Sperm Size

Cells ◽

10.3390/cells10030624 ◽

2021 ◽

Vol 10 (3) ◽

pp. 624

Author(s):

Eliana Pintus ◽

Martin Kadlec ◽

Barbora Karlasová ◽

Marek Popelka ◽

José Luis Ros-Santaella

Keyword(s):

Cell Function ◽

Sperm Quality ◽

Sperm Concentration ◽

Sperm Head ◽

Mitochondrial Activity ◽

Head Width ◽

Dna Integrity ◽

One Year ◽

Sperm Size ◽

Sperm Traits

Tomcats are considered to be adults at 1 year of age, although many reach sexual maturity at an earlier age. Nevertheless, we still know little about whether the spermatogenic activity and sperm quality of mature under one-year-old tomcats differ from those of tomcats that are over one-year-old. This study aims to evaluate the spermatogenic activity, sperm traits, and their relationships in mature tomcats at two different ages. Sixteen tomcats showing complete spermatogenesis and spermatozoa in their epididymal caudae were used and classified according to their age as post-pubertal (<1 year old) or adult (˃1 year old). Our results show that adult cats have higher epididymal sperm concentration and lower coefficient of variation in sperm head width and ellipticity than post-pubertal cats. However, they do not differ in their testicular and epididymal mass, spermatogenesis, and sperm traits such as motility, mitochondrial activity, morphology, morphometry, as well as plasma membrane, acrosome, and DNA integrity. Reduced intra-male variation of sperm head ellipticity is associated with higher testis mass, epididymis mass, and sperm concentration. Interestingly, low intra-male variation in sperm head size is associated with increased Sertoli cell function and reduced post-meiotic germ cell loss. These findings increase our knowledge about feline reproductive physiology and provide new insights into the functional significance of low intra-male variation in sperm size and shape in tomcats.

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Effect of Trehalose and Sucrose in Post-thaw Quality of Crassostrea angulata Sperm

Frontiers in Physiology ◽

10.3389/fphys.2021.749735 ◽

2021 ◽

Vol 12 ◽

Author(s):

Catarina Anjos ◽

Ana Luísa Santos ◽

Daniel Duarte ◽

Domitília Matias ◽

Elsa Cabrita

Keyword(s):

Plasma Membrane ◽

Sperm Quality ◽

Artificial Seawater ◽

Live Cells ◽

Dna Integrity ◽

Computer Assisted ◽

Sperm Cryopreservation ◽

Sperm Analysis ◽

Crassostrea Angulata ◽

And Function

Sperm cryopreservation can be a helpful tool in reproductive management and preservation of biodiversity. However, the freezing methodologies lead to some damage in structure and function of cells that may compromise post-thaw sperm activity. Cryoprotectant supplementation with sugars proved to be a successful strategy to reduce cryodamage in sperm of several species, once allowing to stabilize the plasma membrane constituents. Therefore, this study intends to understand the effects of sugars in the plasma membrane, DNA integrity, and oxidative response during Portuguese oyster sperm cryopreservation. Three cryoprotectants solutions with an initial concentration of 20% dimethyl sulfoxide (DMSO) and 20% DMSO complemented with 0.9 M trehalose or sucrose in artificial seawater were employed. Sperm samples of mature males were individually collected and diluted 1:10 (v/v) in artificial seawater followed by addition of cryoprotectants [1:1 (v/v)]. Thereafter, sperm was loaded into 0.5 ml straws, maintained at 4°C for 10 min, frozen in a programmable biofreezer at −6°C/min from 0 to −70°C, and stored in liquid nitrogen. Samples were thawed in a 37°C bath for 10 s. Several techniques were performed to evaluate post-thaw quality. Sperm motility and DNA integrity were analyzed by using computer-assisted sperm analysis (CASA) software and comet assay. Flow cytometry was employed to determine membrane and acrosome integrity and to detect intracellular reactive oxygen species (ROS) and apoptosis activity. Lipid peroxidation was determined by malondialdehyde (MDA) detection by using spectrophotometry. Sperm antioxidant capacity was evaluated through glutathione peroxidase, glutathione reductase, and superoxide dismutase. Motility was not affected by the extenders containing sugars; these compounds did not reduce the DNA damage. However, both the trehalose and sucrose protected plasma membrane of cells by increasing cell viability and significantly reducing MDA content. The same finding was observed for the ROS, where live cells registered significantly lower levels of ROS in samples cryopreserved with sugars. The activity of antioxidant enzymes was higher in treatments supplemented with sugars, although not significant. In conclusion, the addition of sugars seems to play an important role in protecting the Crassostrea angulata sperm membrane during cryopreservation, showing potential to improve the post-thaw sperm quality and protect the cells from cryoinjuries.

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Treatment of Poor Sperm Quality and Erectile Dysfunction With Oral Pentoxifylline: A Systematic Review

Frontiers in Pharmacology ◽

10.3389/fphar.2021.789787 ◽

2022 ◽

Vol 12 ◽

Author(s):

Yi Lu ◽

Hao Su ◽

Jianzhong Zhang ◽

Yutao Wang ◽

Hongjun Li

Keyword(s):

Systematic Review ◽

Erectile Dysfunction ◽

Male Infertility ◽

Sperm Quality ◽

Sperm Concentration ◽

Treatment Compliance ◽

Infertility Treatment ◽

Cochrane Library ◽

Infertile Men

Background: Pentoxifylline (PTX) is a member of methylxanthine chemicals and a type of non-selective phosphodiesterase-5 inhibitors, which has been used in male infertility treatment to improve sperm quality and erectile dysfunction (ED) treatment. Mutually tight associations existed between ED and male infertility. Using PTX might kill two birds with one stone by improving sperm quality and erectile function in infertile men with ED.Methods: PubMed, Cochrane Library, EMBASE, and Web of Science were searched by October 2021. Based on available evidence from observational studies and randomized-controlled trials (RCTs), we conducted a systematic review to summarize the efficacy and safety of PTX in treating ED and male infertility. The protocol of the article was registered and updated in PROSPERO (CRD42021291396).Results: From 202 records, eight studies (7 RCTs) evaluating the role of PTX in ED and three studies (2 RCTs) assessing the efficacy of PTX in male infertility were included in the systematic review. Three studies (100.00%) and two studies (100.00%) reported the beneficial role of PTX in improving sperm progressive motility and normal sperm morphology rate, respectively. In contrast, only one study (33.33%) indicated the favorable role of PTX in enhancing sperm concentration. As for ED, three (60.00%) studies supported the treatment role of PTX alone in ED, and two studies (66.67%) favored the combination use of PTX and selective PDE5Is compared with selective PDE5Is alone. Safety analysis showed that PTX was a well-tolerated drug in ED and male infertility treatment.Conclusion: Given the association between ED and male infertility and satisfying findings from available evidence, PTX administration for the simultaneous treatment of poor sperm quality and mild ED in infertile men will highly enhance the treatment compliance. However, the finding should be treated carefully until validated by further studies.

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