scholarly journals PlasmiR: A Manual Collection of Circulating microRNAs of Prognostic and Diagnostic Value

Cancers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 3680
Author(s):  
Spyros Tastsoglou ◽  
Marios Miliotis ◽  
Ioannis Kavakiotis ◽  
Athanasios Alexiou ◽  
Eleni C. Gkotsi ◽  
...  
Keyword(s):  
Circulatory System ◽  
Transcriptional Regulators ◽  
Diagnostic Value ◽  
Plasma Samples ◽  
Online Database ◽  
Exclusion Criteria ◽  
Circulating Micrornas ◽  
Circulating Mirna ◽  
Meta Information ◽  
Non Coding Rnas

Only recently, microRNAs (miRNAs) were found to exist in traceable and distinctive amounts in the human circulatory system, bringing forth the intriguing possibility of using them as minimally invasive biomarkers. miRNAs are short non-coding RNAs that act as potent post-transcriptional regulators of gene expression. Extensive studies in cancer and other disease landscapes investigate the protective/pathogenic functions of dysregulated miRNAs, as well as their biomarker potential. A specialized resource amassing experimentally verified, circulating miRNA biomarkers does not exist. We queried the existing literature to identify articles assessing diagnostic/prognostic roles of miRNAs in blood, serum, or plasma samples. Articles were scrutinized in order to exclude instances lacking sufficient experimental documentation or employing no biomarker assessment methods. We incorporated information from more than 200 biomedical articles, annotating crucial meta-information including cohort sizes, inclusion-exclusion criteria, disease/healthy confirmation methods and quantification details. miRNAs and diseases were systematically characterized using reference resources. Our circulating miRNA biomarker collection is provided as an online database, plasmiR. It consists of 1021 entries regarding 251 miRNAs and 112 diseases. More than half of plasmiR’s entries refer to cancerous and neoplastic conditions, 183 of them (32%) describing prognostic associations. plasmiR facilitates smart queries, emphasizing visualization and exploratory modes for all researchers.

103 CIRCULATING microRNAs FOR EARLY DIAGNOSIS OF BOVINE PREGNANCY

2015 ◽  
Vol 27 (1) ◽  
pp. 144
Author(s):  
J. Ioannidis ◽  
C. Ashworth ◽  
R. Raue ◽  
X. Donadeu
Keyword(s):  
Early Diagnosis ◽  
Deep Sequencing ◽  
Mirna Expression ◽  
Plasma Samples ◽  
Circulating Micrornas ◽  
Circulating Mirna ◽  
Time Points ◽  
Bovine Plasma ◽  
Mirna Sequencing ◽  
Pooled Samples

Early diagnosis of pregnancy can shorten calving intervals, improve annual milk production and increase overall profits from modern dairy herds. At present, accurate diagnosis is only possible after the third week of pregnancy. Circulating microRNAs (miRNAs) have been proposed as diagnostic biomarkers for numerous human conditions such as cancer and diabetes. Moreover, distinct circulating miRNA profiles have been associated with different stages of human pregnancy. The objective of this study was to determine whether differential miRNA profiles occur in circulation during early pregnancy (Day 24 or earlier) in cattle that could be used for diagnostic purposes. Holstein-cross heifers were oestrous-synchronised and artificially inseminated (AI, n = 11) or sham-inseminated (control, n = 8) at first detected oestrus. Plasma samples were collected on Days 0, 8, 16 and 24 after insemination. Circulating miRNA levels were independently determined in pooled plasma samples (n = 3 pools for each of pregnant Day 24 and nonpregnant Days 0, 8, and 16) using Qiagen qPCR arrays (Qiagen, Valencia, CA, USA) and in individual samples (n = 11 samples for each pregnant Days 16 and 24, and 8 samples for each of nonpregnant Days 0, 8, and 16, respectively) using Illumina miRNA sequencing. The qPCR array data were analysed using the ΔΔCq method. The miRNA sequencing data were normalised using EdgeR. Differential expression between pregnant and nonpregnant groups was determined using 2-sample t-tests with false discovery rate (FDR) adjustment. Differences in miRNA expression were validated by RT-qPCR. Out of a total of 191 miRNAs analysed in pooled samples using qPCR arrays, 8 were differentially expressed (<3-fold, FDR <0.1) in Day 24 pregnant heifers relative to nonpregnant heifers (Days 0, 8, and 16 combined). No miRNAs were differentially expressed (FDR >0.1) between nonpregnant time-points. Changes in levels of 11 miRNAs were validated by RT-qPCR in individual plasma samples; although expression trends for these miRNAs were the same as in pooled samples, none of the changes in individual samples were significant after FDR adjustment (P > 0.1). Deep sequencing (96 million miRNA reads) identified 231 miRNAs in bovine plasma. There were no significant differences (FDR >0.1) in the expression of any miRNAs between pregnant heifers (Days 16 or 24) and nonpregnant (Days 0, 8, and 16 individually or combined). In addition, no significant differences were identified among nonpregnant time-points. In summary, we successfully performed miRNA profiling of bovine plasma using both deep sequencing and qPCR; however, we did not detect differences in miRNA expression between early pregnant (Day 16 or 24) and nonpregnant heifers. Changes in circulating miRNA levels may involve low abundance miRNAs that cannot be accurately quantified using current technology. Alternatively, changes in circulating miRNA levels may only occur later during pregnancy in cattle.

scholarly journals Integrated miRNA/cytokine/chemokine profiling reveals immunopathological step changes associated with COVID-19 severity

2021 ◽  
Author(s):  
Julie C. WIlson ◽  
David Kealy ◽  
Sally R. James ◽  
Katherine Newling ◽  
Christopher Jagger ◽  
...  
Keyword(s):  
Cell Death ◽  
Correlation Analysis ◽  
Partial Least Squares Regression ◽  
Clinical Parameters ◽  
Plasma Samples ◽  
Least Squares Regression ◽  
Circulating Micrornas ◽  
Circulating Mirna ◽  
Novel Approach ◽  
Insight Into

Circulating microRNAs (miRNAs) are exceptional mechanism-based correlates of disease, yet their potential remains largely untapped in COVID-19. Here, we determined circulating miRNA and cytokine and chemokine (CC) profiles in 171 blood plasma samples from 58 hospitalised COVID-19 patients. Thirty-two miRNAs were differentially expressed in severe cases when compared to moderate and mild cases. These miRNAs and their predicted targets reflected key COVID-19 features including cell death and hypoxia. Compared to mild cases, moderate and severe cases were characterised by a global decrease in circulating miRNA levels. Partial least squares regression using miRNA and CC measurements allowed for discrimination of severe cases with greater accuracy (87%) than using miRNA or CC levels alone. Correlation analysis revealed severity group-specific associations between CC and miRNA levels. Importantly, the miRNAs that correlated with IL6 and CXCL10, two cardinal COVID-19-associated cytokines, were distinct between severity groups, providing a novel qualitative way to stratify patients with similar levels of proinflammatory cytokines but different disease severity. Integration of miRNA and CC levels with clinical parameters revealed severity-specific signatures associated with clinical hallmarks of COVID-19. Our study highlights the existence of severity-specific circulating CC/miRNA networks, providing insight into COVID-19 pathogenesis and a novel approach for monitoring COVID-19 progression.

scholarly journals Protocol to analyze circulating small non-coding RNAs by high-throughput RNA sequencing from human plasma samples

2021 ◽  
Vol 2 (3) ◽  
pp. 100606
Author(s):  
Giuseppina E. Grieco ◽  
Guido Sebastiani ◽  
Daniela Fignani ◽  
Noemi Brusco ◽  
Laura Nigi ◽  
...  
Keyword(s):  
Human Plasma ◽  
Rna Sequencing ◽  
High Throughput ◽  
Plasma Samples ◽  
Human Plasma Samples ◽  
Non Coding Rnas

Circulating miR-126 as a Potential Non-invasive Biomarker for Intracranial Aneurysmal Rupture: A Pilot Study

2021 ◽  
Vol 19 ◽  
Author(s):  
Jianing Wu ◽  
Ilgiz Gareev ◽  
Ozal Beylerli ◽  
Albert Mukhamedzyanov ◽  
Valentin Pavlov ◽  
...  
Keyword(s):  
Aneurysmal Subarachnoid Hemorrhage ◽  
Diagnostic Value ◽  
Plasma Samples ◽  
Expression Levels ◽  
Time Points ◽  
Non Invasive ◽  
Risk Of Rupture ◽  
Life Threatening ◽  
Healthy Control ◽  
New Biomarkers

Aim: Intracranial aneurysms (IAs) are characterized by abnormal dilation and thinning of the cerebral vessels wall, leading to rupture and life-threatening aneurysmal subarachnoid hemorrhage (aSAH) condition. This dictates the need to find new biomarkers that predict the presence of IAs and the risk of their rupture. The aim of this study was to measure circulating miR-126 at various time points post-aSAH to identify the timing of peak levels. Methods: Plasma samples from 62 patients with unruptured IAs (UIAs), 80 patients with aSAH at various time points (1, 3, 7, and 14 days post-event), and 47 healthy control were collected and subjected to qRT-PCR analyses for the expression levels of circulating miR-126. ROC curve and AUC were used to evaluate the diagnostic value of circulating miR-126. Results: The expression levels of circulating miR-126 were increased in patients with UIAs than in the healthy control. Furthermore, the expression levels of circulating miR-126 rose substantially from day 1 to day 7, but with a moderate decrease from day 7 to day 14 in plasma of patients with aSAH. The peak was observed on day 7. The AUC for miR-126 was 0.75, 0.75, 0.82, 0.87, and 0.79, respectively, and demonstrated that circulating miR-126 displayed considerable accuracy in discriminating plasma of patients with UIAs and patients after aSAH at various time points from a healthy control. Conclusion: Our results indicated that circulating miR-126 in plasma samples could be served as a potential non-invasive biomarker in IAs detection and prevention IAs with a high risk of rupture.

HUBUNGAN INTENSITAS KEBISINGAN DENGAN TEKANAN DARAH PADA PEKERJA AREA WORKSHOP

2020 ◽  
Vol 9 (1) ◽  
pp. 18-26
Author(s):  
Mariman Tjendera ◽  
Isramilda Isramilda
Keyword(s):  
Blood Pressure ◽  
Noise Intensity ◽  
Circulatory System ◽  
Sampling Technique ◽  
Strong Relationship ◽  
Normal Blood ◽  
Normal Blood Pressure ◽  
Exclusion Criteria ◽  
Cross Sectional ◽  
Stage 1

Noise in the workplace is often a separate problem for the workforce so that it can cause mental-emotional disturbance as well as the heart and circulatory system. According to the 2013 Basic Health Research, the prevalence of high blood pressure in Indonesia at the age of ≥18 years is 25.8%. So the researchers wanted to find a relationship between noise intensity and blood pressure. This research method was observational analytic with a cross-sectional approach conducted at PT. Bintang Intipersada Shipyard, Batam City. The sampling technique was a total sampling with a population of 100 workers in 2018 and obtained results of 61 workers determined by inclusion and exclusion criteria. The results of the study were analyzed with a frequency distribution tabulated and tested with the Pearson Product Moment Test. The results of this study worker who were exposed to noise intensity ≤85 dBA had a normal blood pressure of 8 (36.5%) people, in workers who were exposed to noise intensity ≤85 dBA had blood pressure with Pre-hypertension as many as 12 (54.5% ) people, for workers exposed to noise intensity ≤85 dBA have 1 (4.5%) blood pressure with Stage 1 Hypertension, workers who are exposed to noise intensity ≤85 dBA have blood pressure with Stage 2 Hypertension as much as 1 (4.5 %) person. While workers who are exposed to noise intensity> 85 dBA have normal blood pressure of 0 (0%) people, workers who are exposed to noise intensity> 85 dBA have blood pressure with Pre-hypertension as much as 2 (5.1%) people, workers are exposed to noise intensity> 85 dBA had blood pressure with Stage 1 Hypertension as many as 14 (35.9%) people, then workers exposed to noise intensity> 85 dBA had blood pressure with Stage 2 Hypertension as many as 23 (59%) workers. The results of the Pearson Product Moment analysis value of p = 0,000 <α 0.05, there is a relationship between noise intensity and blood pressure with the magnitude of the correlation coefficient (r) which is 0.795 meaning, there is a strong relationship. Based on this study it can be concluded that there is a significant relationship between noise intensity and blood pressure.

Cerebrospinal fluid microRNAs as diagnostic biomarkers in brain tumors

2018 ◽  
Vol 56 (6) ◽  
pp. 869-879 ◽  
Author(s):  
Alena Kopkova ◽  
Jiri Sana ◽  
Pavel Fadrus ◽  
Ondrej Slaby
Keyword(s):  
Cerebrospinal Fluid ◽  
Brain Tumors ◽  
Communication Channel ◽  
Predictive Biomarkers ◽  
Waste Products ◽  
Diagnostic Biomarkers ◽  
Circulating Micrornas ◽  
The Central Nervous System ◽  
Non Coding Rnas ◽  
Extracellular Environment

Abstract Cerebrospinal fluid (CSF) is a body fluid that has many important functions and is in direct contact with the extracellular environment of the central nervous system (CNS). CSF serves as both the communication channel allowing the distribution of various substances among the CNS cells and the storage facility for the waste products these cells release. For these reasons, CSF is a potential source of diagnostic biomarkers of many CNS diseases, including brain tumors. Recent studies have revealed that CSF also contains circulating microRNAs (miRNAs), short non-coding RNAs that have been described as biomarkers in many cancers. However, CSF miRNAs are difficult to detect, which is why researchers face major challenges, including technological difficulties in its detection and its lack of standardization. Therefore, this review aims (i) to highlight the potential of CSF miRNAs as diagnostic, prognostic and predictive biomarkers in brain tumors, and (ii) to summarize technological approaches for detection of CSF miRNAs.

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