scholarly journals Computational Assessment of Botrytis cinerea Lipase for Biofuel Production

Catalysts ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1319
Author(s):  
Tehsin Fatma ◽  
Zeeshan Zafar ◽  
Sidra Fatima ◽  
Rehan Zafar Paracha ◽  
Fazal Adnan ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Botrytis Cinerea ◽  
Fossil Fuel ◽  
Hydrophobic Interactions ◽  
Phylogenetic Analyses ◽  
Trienoic Acid ◽  
Biofuel Production ◽  
Binding Affinities ◽  
Lipase Catalysis

The demand for ecofriendly green catalysts for biofuel synthesis is greatly increasing with the effects of fossil fuel depletion. Fungal lipases are abundantly used as biocatalysts for the synthesis of biofuel. The use of Botrytis cinerea lipase is an excellent approach for the conversion of agroindustrial residues into biofuel. In this study, phylogenetic analyses were carried out and the physicochemical properties of B. cinerea lipase were assessed. Furthermore, the protein structure of B. cinerea lipase was predicted and refined. Putative energy-rich phytolipid compounds were explored as a substrate for the synthesis of biofuel, owing to B. cinerea lipase catalysis. Approximately 161 plant-based fatty acids were docked with B. cinerea lipase in order to evaluate their binding affinities and interactions. Among the docked fatty acids, the top ten triglycerides having the lowest number of binding affinities with B. cinerea lipase were selected, and their interactions were assessed. The top three triglycerides having the greatest number of hydrogen bonds and hydrophobic interactions were selected for simulations of 20 ns. The docking and simulations revealed that docosahexaenoic acid, dicranin, and hexadeca-7,10,13-trienoic acid had stable bonding with the B. cinerea lipase. Therefore, B. cinerea lipase has the potential to be used for the transesterification of fatty acids into biofuels, whereas docosahexaenoic acid, dicranin, and hexadeca-7,10,13-trienoic acid can be used as substrates of B. cinerea lipase for biofuel synthesis.

Thraustochytrids from the Red Sea mangroves in Saudi Arabia and their abilities to produce docosahexaenoic acid

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Mohamed A. Abdel-Wahab ◽  
Abd El-Rahim M.A. El-Samawaty ◽  
Abdallah M. Elgorban ◽  
Ali H. Bahkali
Keyword(s):  
Fatty Acids ◽  
Saudi Arabia ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Docosahexaenoic Acid ◽  
Red Sea ◽  
Large Scale ◽  
Phylogenetic Analyses ◽  
Dry Weight ◽  
Rdna Sequences

Abstract This is the first study of thraustochytrids from the Red Sea coast in Saudi Arabia. One hundred and thirty-four isolates of thraustochytids were cultured from Al-Leith, Jeddah and Yanbu mangroves from this area, and were categorized into 38 morphotypes. Among the isolated thraustochytrids, Aurantiochytrium was the most speciose genus, represented by 36 morphological types. Seventeen strains formed a distinct clade within the genus Aurantiochytrium based on phylogenetic analyses of 18S rDNA sequences. The Aurantiochytrium clade from the Middle East is characterized by the production of high levels of oleic and linoleic acids and may represent undescribed taxa. Four Aurantiochytrium strains were grown on large scale to study their ability to produce docosahexaenoic acid (DHA). These strains produced biomass ranging from 37.7 to 66 g L−1 and the percentages of oil ranged from 40 to 57.2% of the dry weight. Twenty-one fatty acids were recorded from the four strains which included: eight saturated four monounsaturated and nine polyunsaturated. Dominant fatty acids included C16:0 palmitic acid (24.14–37.02% total fatty acid (TFA)), C18:1ω9 oleic acid (3.01–25.07% TFA), C18:2ω6 linoleic acid (1.85–20.85% TFA) and C22:6ω3 DHA (4.87–16.5% TFA). DHA was the predominant polyunsaturated fatty acid in three strains, while linoleic acid was the predominant PUFAs in one strain.

scholarly journals A Modelling Framework for the Conceptual Design of Low-Emission Eco-Industrial Parks in the Circular Economy: A Case for Algae-Centered Business Consortia

Water ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 69
Author(s):  
Aldric S. Tumilar ◽  
Dia Milani ◽  
Zachary Cohn ◽  
Nick Florin ◽  
Ali Abbas
Keyword(s):  
Circular Economy ◽  
Carbon Capture ◽  
Fossil Fuel ◽  
Production Systems ◽  
Flow Analysis ◽  
Biofuel Production ◽  
Industrial Symbiosis ◽  
Modelling Framework ◽  
Low Emission ◽  
Energy Exchanges

This article describes a unique industrial symbiosis employing an algae cultivation unit (ACU) at the core of a novel eco-industrial park (EIP) integrating fossil-fuel fired power generation, carbon capture, biofuel production, aquaculture, and wastewater treatment. A new modelling framework capable of designing and evaluating materials and energy exchanges within an industrial eco-system is introduced. In this scalable model, an algorithm was developed to balance the material and energy exchanges and determine the optimal inputs and outputs based on the industrial symbiosis objectives and participating industries. Optimizing the functionality of the ACU not only achieved a substantial emission reduction, but also boosted aquaculture, biofuel, and other chemical productions. In a power-boosting scenario (PBS), by matching a 660 MW fossil fuel-fired power plant with an equivalent solar field in the presence of ACU, fish-producing aquaculture and biofuel industries, the net CO2 emissions were cut by 60% with the added benefit of producing 39 m3 biodiesel, 6.7 m3 bioethanol, 0.14 m3 methanol, and 19.55 tons of fish products annually. Significantly, this article shows the potential of this new flexible modelling framework for integrated materials and energy flow analysis. This integration is an important pathway for evaluating energy technology transitions towards future low-emission production systems, as required for a circular economy.

DHA-enriched phospholipids from large yellow croaker roe regulate lipid metabolic disorders and gut microbiota imbalance on SD rats with a high-fat diet

2021 ◽  
Author(s):  
Xiaodan Lu ◽  
Rongbin Zhong ◽  
Ling Hu ◽  
Luyao Huang ◽  
Lijiao Chen ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Gut Microbiota ◽  
Polyunsaturated Fatty Acids ◽  
High Fat Diet ◽  
Nutritional Value ◽  
Metabolic Disorders ◽  
Large Yellow Croaker ◽  
High Fat ◽  
Sd Rats

Abstract Large yellow croaker roe phospholipids (LYCRPLs) has great nutritional value because of containing rich docosahexaenoic acid (DHA), which is a kind of n-3 polyunsaturated fatty acids (n-3 PUFAs). In...

scholarly journals Microalgae n-3 PUFAs Production and Use in Food and Feed Industries

Marine Drugs ◽  
2021 ◽  
Vol 19 (2) ◽  
pp. 113
Author(s):  
Marine Remize ◽  
Yves Brunel ◽  
Joana L. Silva ◽  
Jean-Yves Berthon ◽  
Edith Filaire
Keyword(s):  
Fatty Acids ◽  
Global Warming ◽  
Docosahexaenoic Acid ◽  
Polyunsaturated Fatty Acids ◽  
Eicosapentaenoic Acid ◽  
Human Health ◽  
Current Knowledge ◽  
Epa And Dha ◽  
Anti Cancer ◽  
Food And Feed

N-3 polyunsaturated fatty acids (n-3 PUFAs), and especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are essential compounds for human health. They have been proven to act positively on a panel of diseases and have interesting anti-oxidative, anti-inflammatory or anti-cancer properties. For these reasons, they are receiving more and more attention in recent years, especially future food or feed development. EPA and DHA come mainly from marine sources like fish or seaweed. Unfortunately, due to global warming, these compounds are becoming scarce for humans because of overfishing and stock reduction. Although increasing in recent years, aquaculture appears insufficient to meet the increasing requirements of these healthy molecules for humans. One alternative resides in the cultivation of microalgae, the initial producers of EPA and DHA. They are also rich in biochemicals with interesting properties. After defining macro and microalgae, this review synthesizes the current knowledge on n-3 PUFAs regarding health benefits and the challenges surrounding their supply within the environmental context. Microalgae n-3 PUFA production is examined and its synthesis pathways are discussed. Finally, the use of EPA and DHA in food and feed is investigated. This work aims to define better the issues surrounding n-3 PUFA production and supply and the potential of microalgae as a sustainable source of compounds to enhance the food and feed of the future.

Influence of dietary docosahexaenoic acid supplementation on the overall rumen microbiota of dairy cows and linkages with production parameters

2014 ◽  
Vol 60 (5) ◽  
pp. 267-275 ◽  
Author(s):  
Valeria A. Torok ◽  
Nigel J. Percy ◽  
Peter J. Moate ◽  
Kathy Ophel-Keller
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Docosahexaenoic Acid ◽  
Dairy Cows ◽  
Bacterial Communities ◽  
Ciliate Protozoan ◽  
Rumen Microbiota ◽  
Production Parameters ◽  
Enteric Methane ◽  
Archaeal Communities

The rumen microbiota contributes to greenhouse gas emissions and has an impact on feed efficiency and ruminant product fatty acid composition. Dietary fat supplements have shown promise in reducing enteric methane production and in altering the fatty acid profiles of ruminant-derived products, yet in vivo studies on how these impact the rumen microbiota are limited. In this study, we investigated the rumen bacterial, archaeal, fungal, and ciliate protozoan communities of dairy cows fed diets supplemented with 4 levels of docosahexaenoic acid (DHA) (0, 25, 50, and 75 g·cow−1·day−1) and established linkages between microbial communities and production parameters. Supplementation with DHA significantly (P < 0.05) altered rumen bacterial and archaeal, including methanogenic archaeal, communities but had no significant (P > 0.05) effects on rumen fungal or ciliate protozoan communities. Rumen bacterial communities of cows receiving no DHA were correlated with increased saturated fatty acids (C18:0 and C11:0) in their milk. Furthermore, rumen bacterial communities of cows receiving a diet supplemented with 50 g DHA·cow−1·day−1 were correlated with increases in monounsaturated fatty acids (C20:1n-9) and polyunsaturated fatty acids (C22:5n-3; C22:6n-3; C18:2 cis-9, trans-11; C22:3n-6; and C18:2n-6 trans) in their milk. The significant diet-associated changes in rumen archaeal communities observed did not result in altered enteric methane outputs in these cows.

Lipase-catalyzed esterification of glycerol with n-3 polyunsaturated fatty acids from winterized fish oil Esterificación catalítica de glicerol con ácidos grasos 3-n poliinsaturados de aceite de pescado

1998 ◽  
Vol 4 (6) ◽  
pp. 401-405 ◽  
Author(s):  
V.J. Robles ◽  
H.S. García ◽  
J.A. Monroy ◽  
O. Angulo
Keyword(s):  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Polyunsaturated Fatty Acids ◽  
Eicosapentaenoic Acid ◽  
Phosphate Buffer ◽  
Esterification Reaction ◽  
Pseudomonas Sp ◽  
Menhaden Oil ◽  
Glyceride Synthesis ◽  
Esterified Fatty Acids

Menhaden oil was hydrolyzed using a lipase from Pseudomonas sp. The hydrolysate was cold frac tionated at-72°C. Glyceride synthesis was performed using the same lipase under different reaction environments. The best conditions for the esterification reaction were 39 °C for 18 h in a reaction mixture containing anhydrous glycerol, n-3 polyunsaturated fatty acids (PUFA) enriched solution (2% lipids in hexane), hexane, and phosphate buffer-lipase solution (1% w/v). Product composition was 81.33% triacylglycerides and 18.67% of free fatty acids (w/w). Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) accounted for 36.18% of the esterified fatty acids, of which 58% was EPA and 42% was DHA. This method offers an alternative to produce glycerides rich in n-3 PUFA.

scholarly journals The Use of Urea and Kelp Waste Extract is A Promising Strategy for Maximizing the Biomass Productivity and Lipid Content in Chlorella sorokiniana

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 463 ◽  
Author(s):  
Ali Nawaz Kumbhar ◽  
Meilin He ◽  
Abdul Razzaque Rajper ◽  
Khalil Ahmed Memon ◽  
Muhammad Rizwan ◽  
...  
Keyword(s):  
Lipid Content ◽  
Fossil Fuels ◽  
Fossil Fuel ◽  
Lipid Production ◽  
Total Lipid Content ◽  
Biomass Productivity ◽  
Chlorella Sorokiniana ◽  
Biofuel Production ◽  
Promising Strategy ◽  
Waste Extract

The decline in fossil fuel reserves has forced researchers to seek out alternatives to fossil fuels. Microalgae are considered to be a promising feedstock for sustainable biofuel production. Previous studies have shown that urea is an important nitrogen source for cell growth and the lipid production of microalgae. The present study investigated the effect of different concentrations of urea combined with kelp waste extract on the biomass and lipid content of Chlorella sorokiniana. The results revealed that the highest cell density, 20.36 × 107 cells−1, and maximal dry biomass, 1.70 g/L, were achieved in the presence of 0.5 g/L of urea combined with 8% kelp waste extract. Similarly, the maximum chlorophyll a, b and beta carotenoid were 10.36 mg/L, 7.05, and 3.01 mg/L, respectively. The highest quantity of carbohydrate content, 290.51 µg/mL, was achieved in the presence of 0.2 g/L of urea and 8% kelp waste extract. The highest fluorescence intensity, 40.05 × 107 cells−1, and maximum total lipid content (30%) were achieved in the presence of 0.1 g/L of urea and 8% kelp waste extract. The current study suggests that the combination of urea and kelp waste extract is the best strategy to enhance the biomass and lipid content in Chlorella sorokiniana.

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