Preliminary Phytochemical Analysis and Evaluation of the Biological Activity of Leonotis nepetifolia (L.) R. Br Transformed Roots Extracts Obtained through Rhizobium rhizogenes-Mediated Transformation

According to the present knowledge, this is the first report on establishing transformed root cultures of Leonotis nepetifolia after Rhizobium rhizogenes-mediated transformation. The preliminary phytochemical analysis showed differences in the content of phenols and flavonoids in transformed and nontransformed roots. The dominant compounds in the analyzed extracts were (+)-catechin (5464 and 6808 µg/g DW), p-coumaric acid (2549 and 4907 µg/g DW), m-coumaric acid (1508 and 2048 µg/g DW) and rosmarinic acid (1844 and 2643 µg/g DW) for nontransformed (LNNR) and transformed (LNTR4) roots, respectively. Initial biological studies carried out on LNNR, and LNTR4 extracts showed a cytotoxic effect on the A549 lung, HCC1937 breast and leukemia NALM-6 cell lines, antioxidants, as well as repair and protection against DNA damage induced by H2O2 in HUVEC cells. Due to the stronger effect of the LNTR4 root extract, which can be a relatively efficient and cheap source of bioactive secondary metabolites, further biological analyses are needed to discover in detail their potentially valuable biological properties.

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Phytochemical screening and antibacterial effect of root extract of Boerhaavia diffusa L. (Family Nyctaginaceae)

Journal of Applied and Natural Science ◽

10.31018/jans.v5i1.310 ◽

2013 ◽

Vol 5 (1) ◽

pp. 221-225 ◽

Cited By ~ 2

Author(s):

Deepti Malhotra ◽

Amir Khan ◽

Fouzia Ishaq

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Effect ◽

Phytochemical Analysis ◽

Root Extract ◽

Bacterial Strains ◽

Zone Of Inhibition ◽

Analysis And Evaluation ◽

Root Extracts ◽

The Family ◽

Boerhaavia Diffusa

Boerhaavia diffusa belonging to the family of the Nyctaginaceae is mainly a diffused perennial herbaceous creeping weed of India. The present study deals with the investigation of phytochemical analysis and evaluation of the antimicrobial activity of the aqueous and methanolic extract of the roots of Boerhaavia diffusa. The result revealed the presence of alkaloid, glycoside, saponins, flavonoids, polysaccharides, steroid and tannin in both the root extracts. B. diffusa root extract possesses antimicrobial activity as the zone of inhibition was observed for both gram positive as well as gram negative bacterial strains.

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Phytochemical Analysis and Antimicrobial Activity of Origanum dictamnus Traditional Herbal Tea (decoction)

Natural Product Communications ◽

10.1177/1934578x1701201139 ◽

2017 ◽

Vol 12 (11) ◽

pp. 1934578X1701201

Author(s):

Myrto Varsani ◽

Konstantia Graikou ◽

Aristea Velegraki ◽

Ioanna Chinou

Keyword(s):

Antimicrobial Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Biological Properties ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Coumaric Acid ◽

Herbal Tea ◽

Three Samples ◽

First Time

In the framework of the re-evaluation of chemistry and biological properties of Greek herbal teas (decoctions), the objective of this study was the estimation of the total phenolic content and the antimicrobial activity as well as the chemical analysis of the water extracts (decoction) of three samples of Cretan dittany ( Origanum dictamnus L) prepared in the traditional way to be used (drunk). Ten secondary metabolites were isolated and elucidated on the basis of spectral evidence for the first time from O. dictamnus drinkable decoction: carvacrol (1), β-sitosterol (2), uvaol (3), ursolic acid (4), rosmarinic acid (5), lithospermic acid (6), ferulic acid (7), cafeic acid (8), p-coumaric acid (9) and apigenin-7- O-glucoside (10). The samples showed a high total phenolic content (185.5-139.7 mg CAE/g) and an interesting antibacterial profile against Gram positive and negative human pathogenic microorganisms. Furthermore, the bioactivity against a panel of Malassezia strains was estimated.

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Tamarix articulata (T. articulata) - An Important Halophytic Medicinal Plant with Potential Pharmacological Properties

Current Pharmaceutical Biotechnology ◽

10.2174/1389201020666190318120103 ◽

2019 ◽

Vol 20 (4) ◽

pp. 285-292 ◽

Cited By ~ 5

Author(s):

Abdullah M. Alnuqaydan ◽

Bilal Rah

Keyword(s):

Medicinal Plant ◽

Biological Activities ◽

Wide Spectrum ◽

Biological Properties ◽

In Vivo Model ◽

Drug Candidate ◽

Phytochemical Analysis ◽

Pharmacological Properties

Background:Tamarix Articulata (T. articulata), commonly known as Tamarisk or Athal in Arabic region, belongs to the Tamaricaece species. It is an important halophytic medicinal plant and a good source of polyphenolic phytochemical(s). In traditional medicines, T. articulata extract is commonly used, either singly or in combination with other plant extracts against different ailments since ancient times.Methods:Electronic database survey via Pubmed, Google Scholar, Researchgate, Scopus and Science Direct were used to review the scientific inputs until October 2018, by searching appropriate keywords. Literature related to pharmacological activities of T. articulata, Tamarix species, phytochemical analysis of T. articulata, biological activities of T. articulata extracts. All of these terms were used to search the scientific literature associated with T. articulata; the dosage of extract, route of administration, extract type, and in-vitro and in-vivo model.Results:Numerous reports revealed that T. articulata contains a wide spectrum of phytochemical(s), which enables it to have a wide window of biological properties. Owing to the presence of high content of phytochemical compounds like polyphenolics and flavonoids, T. articulata is a potential source of antioxidant, anti-inflammatory and antiproliferative properties. In view of these pharmacological properties, T. articulata could be a potential drug candidate to treat various clinical conditions including cancer in the near future.Conclusion:In this review, the spectrum of phytochemical(s) has been summarized for their pharmacological properties and the mechanisms of action, and the possible potential therapeutic applications of this plant against various diseases discussed.

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Gynura procumbens Root Extract Ameliorates Ischemia-Induced Neuronal Damage in the Hippocampal CA1 Region by Reducing Neuroinflammation

Nutrients ◽

10.3390/nu13010181 ◽

2021 ◽

Vol 13 (1) ◽

pp. 181

Author(s):

Woosuk Kim ◽

Hyo Young Jung ◽

Dae Young Yoo ◽

Hyun Jung Kwon ◽

Kyu Ri Hahn ◽

...

Keyword(s):

Inflammatory Responses ◽

Neuronal Damage ◽

Ischemia Reperfusion ◽

Treated Group ◽

Biological Properties ◽

Root Extract ◽

Ischemic Damage ◽

Neuroprotective Effects ◽

Hippocampal Ca1 ◽

Vehicle Treated Group

Gynura procumbens has been used in Southeast Asia for the treatment of hypertension, hyperglycemia, and skin problems induced by ultraviolet irradiation. Although considerable studies have reported the biological properties of Gynura procumbens root extract (GPE-R), there are no studies on the effects of GPE-R in brain damages, for example following brain ischemia. In the present study, we screened the neuroprotective effects of GPE-R against ischemic damage and neuroinflammation in the hippocampus based on behavioral, morphological, and biological approaches. Gerbils received oral administration of GPE-R (30 and 300 mg/kg) every day for three weeks and 2 h after the last administration, ischemic surgery was done by occlusion of both common carotid arteries for 5 min. Administration of 300 mg/kg GPE-R significantly reduced ischemia-induced locomotor hyperactivity 1 day after ischemia. Significantly more NeuN-positive neurons were observed in the hippocampal CA1 regions of 300 mg/kg GPE-R-treated animals compared to those in the vehicle-treated group 4 days after ischemia. Administration of GPE-R significantly reduced levels of pro-inflammatory cytokines such as interleukin-1β, -6, and tumor necrosis factor-α 6 h after ischemia/reperfusion. In addition, activated microglia were significantly decreased in the 300 mg/kg GPE-R-treated group four days after ischemia/reperfusion compared to the vehicle-treated group. These results suggest that GPE-R may be one of the possible agents to protect neurons from ischemic damage by reducing inflammatory responses.

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Extracts from Pulsatilla patens target cancer-related signaling pathways in HeLa cells

Scientific Reports ◽

10.1038/s41598-021-90136-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Grażyna Łaska ◽

Magdalena Maciejewska-Turska ◽

Elwira Sieniawska ◽

Łukasz Świątek ◽

David S. Pasco ◽

...

Keyword(s):

Signaling Pathways ◽

Hela Cells ◽

Luciferase Reporter ◽

Cervical Cancer Cell Line ◽

Phytochemical Analysis ◽

Root Extract ◽

Triterpenoid Saponins ◽

Cancer Signaling ◽

Methanolic Extracts ◽

Pulsatilla Patens

AbstractThe purpose of this study was to determine if a methanolic extract of the Pulsatilla patens (L.) Mill. can inhibit the progression of cancer through the modulation of cancer-related metabolic signaling pathways. We analyzed a panel of 13 inducible luciferase reporter gene vectors which expression is driven by enhancer elements that bind to specific transcription factors for the evaluation of the activity of cancer signaling pathways. The root extract of P. patens exhibited strong inhibition of several signaling pathways in HeLa cells, a cervical cancer cell line, and was found to be the most potent in inhibiting the activation of Stat3, Smad, AP-1, NF-κB, MYC, Ets, Wnt and Hdghog, at a concentration of 40 µg/mL. The methanolic extracts of P. patens enhanced apoptotic death, deregulated cellular proliferation, differentiation, and progression towards the neoplastic phenotype by altering key signaling molecules required for cell cycle progression. This is the first study to report the influence of Pulsatilla species on cancer signaling pathways. Further, our detailed phytochemical analysis of the methanolic extracts of the P. patens allowed to deduce that compounds, which strongly suppressed the growth and proliferation of HeLa cancer cells were mainly triterpenoid saponins accompanied by phenolic acids.

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Update on Phytochemical and Biological Studies on Rocaglate Derivatives from Aglaia Species

Planta Medica ◽

10.1055/a-1401-9562 ◽

2021 ◽

Author(s):

Garima Agarwal ◽

Long-Sheng Chang ◽

Djaja Doel Soejarto ◽

A. Douglas Kinghorn

Keyword(s):

Biological Activities ◽

Chemical Constituents ◽

Protein Translation ◽

Biological Properties ◽

Translation Initiation Factor ◽

Initiation Factor ◽

Eukaryotic Translation ◽

Ability To Act ◽

Biological Studies ◽

Phytochemical Constituents

AbstractWith about 120 species, Aglaia is one of the largest genera of the plant family Meliaceae (the mahogany plants). It is native to the tropical rainforests of the Indo-Australian region, ranging from India and Sri Lanka eastward to Polynesia and Micronesia. Various Aglaia species have been investigated since the 1960s for their phytochemical constituents and biological properties, with the cyclopenta[b]benzofurans (rocaglates or flavaglines) being of particular interest. Phytochemists, medicinal chemists, and biologists have conducted extensive research in establishing these secondary metabolites as potential lead compounds with antineoplastic and antiviral effects, among others. The varied biological properties of rocaglates can be attributed to their unusual structures and their ability to act as inhibitors of the eukaryotic translation initiation factor 4A (eIF4A), affecting protein translation. The present review provides an update on the recently reported phytochemical constituents of Aglaia species, focusing on rocaglate derivatives. Furthermore, laboratory work performed on investigating the biological activities of these chemical constituents is also covered.

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Strobilanthes heyneanus root extract as a potential source for antioxidant and antimicrobial activity

Future Journal of Pharmaceutical Sciences ◽

10.1186/s43094-021-00242-2 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Vijayakumar Sundaram ◽

Selvaraju Sadhasivam ◽

Sivaraj Chandrasekaran ◽

Raaman Nanjian ◽

Arjun Pandian

Keyword(s):

Dermal Fibroblast ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Fibroblast Cell ◽

Phytochemical Analysis ◽

Root Extract ◽

Scavenging Activity ◽

Medicinal Uses

Abstract Background Strobilanthes heyneanus (Nilgirianthus heyneanus) belongs to the family Acanthaceae that contains many species with potential for diverse medicinal uses. It is also called ‘Karun kurinji’ and is commonly found in the South-West regions of India. The species are commonly used in rheumatic complaints, sprain of the ankle, and hernia. The objectives of the study were to evaluate the antioxidant activity, phytochemical analysis, and antibacterial activities of the root extract of S. heyneanus. Results The radical scavenging and reduction assays such as DPPH● radical and OH● radical scavenging assays, as well as phosphomolybdenum reduction and Fe3+ reducing power assays, were determined for the root extract. The highest DPPH● radical scavenging activity was 88.23 ± 1.32 at 120 μg/mL concentration, and the calculated IC50 was 38.52 μg/mL concentration. The highest OH● radical scavenging activity was 51.28 ± 1.06 at 120 μg/mL concentration, and the calculated IC50 was 51.28 μg/mL concentration. The highest ABTS●+ radical scavenging activity was 91.28 ± 1.12 at 30 μg/mL concentration, and the calculated IC50 was 33.92 μg/mL concentration. The highest phosphomolybdenum reduction was 87.43 ± 0.90 at 120 μg/mL concentration, and the calculated RC50 was 24.74 μg/mL concentration. The highest Fe3+ reduction was 89.38 ± 0.98 at 120 μg/mL concentration, and the calculated RC50 was 31.06 μg/mL concentration. The antibacterial activity of S. heyneanus showed the highest zone of inhibition of 24 mm for Salmonella typhi with 500 μg/mL concentration. The cytotoxicity limits of the root extracts were tested by MTT assay using human dermal fibroblast cell lines, reflecting > 90% cell viability at a concentration of 500 μg/mL. Conclusions The current study showed that the root extract of S. heyneanus has better antioxidant properties and potential anti-bacterial compounds. The phytochemical analysis of the root extract showed the presence of alkaloids, steroids, terpenoids, phenols, flavonoids, tannins, saponins, glycosides, and carbohydrates, which are responsible for the antibacterial root extract of S. heyneanus synergistically.

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Development of Spanish Broom and Flax Dressings with Glycyrrhetinic Acid-Loaded Films for Wound Healing: Characterization and Evaluation of Biological Properties

Pharmaceutics ◽

10.3390/pharmaceutics13081192 ◽

2021 ◽

Vol 13 (8) ◽

pp. 1192

Author(s):

Angela Abruzzo ◽

Concettina Cappadone ◽

Valentina Sallustio ◽

Giovanna Picone ◽

Martina Rossi ◽

...

Keyword(s):

Wound Healing ◽

Drug Loading ◽

Healing Process ◽

In Vitro Release ◽

Sodium Hyaluronate ◽

Biological Properties ◽

Glycyrrhetinic Acid ◽

Wound Dressings ◽

Biological Studies

The selection of an appropriate dressing for each type of wound is a very important procedure for a faster and more accurate healing process. So, the aim of this study was to develop innovative Spanish Broom and flax wound dressings, as alternatives to cotton used as control, with polymeric films containing glycyrrhetinic acid (GA) to promote wound-exudate absorption and the healing process. The different wound dressings were prepared by a solvent casting method, and characterized in terms of drug loading, water uptake, and in vitro release. Moreover, biological studies were performed to evaluate their biocompatibility and wound-healing efficacy. Comparing the developed wound dressings, Spanish Broom dressings with GA-loaded sodium hyaluronate film had the best functional properties, in terms of hydration ability and GA release. Moreover, they showed a good biocompatibility, determining a moderate induction of cell proliferation and no cytotoxicity. In addition, the wound-healing test revealed that the Spanish Broom dressings promoted cell migration, further facilitating the closure of the wound.

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Antihepatotoxic Activity of Phyllanthus atropurpureus Cultivated in Egypt

Zeitschrift für Naturforschung C ◽

10.1515/znc-2011-9-1002 ◽

2011 ◽

Vol 66 (9-10) ◽

pp. 447-452 ◽

Cited By ~ 1

Author(s):

Taha Sarg ◽

Afaf Abdel Ghani ◽

Rawia Zayed ◽

May El-Sayed

Keyword(s):

Liver Injury ◽

Active Ingredient ◽

Ornamental Plants ◽

Hepatoprotective Activity ◽

Maximum Activity ◽

Phytochemical Analysis ◽

Root Extract ◽

Aerial Parts ◽

Serum Aspartate Aminotransferase ◽

Antihepatotoxic Activity

The genus Phyllanthus (family Euphorbiaceae) is considered one of the important medicinal and ornamental plants. A phytochemical analysis of the extracts was performed to search for the active ingredient. Results of the investigation of the hepatoprotective activity of Phyllanthus atropurpureus Boj. Hort. Maurit. revealed that the activities of alcoholic extracts of its aerial parts and roots were quite similar to those of silymarin. Both of them improve the parameters of CCl4-induced liver injury including serum aspartate aminotransferase and alanine aminotransferase. Among the extracts tested, the root extract showed maximum activity compared to the aerial parts extract and to silymarin.

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Origanum vulgare ssp. vulgare: Chemical Composition and Biological Studies

Molecules ◽

10.3390/molecules23082077 ◽

2018 ◽

Vol 23 (8) ◽

pp. 2077 ◽

Cited By ~ 17

Author(s):

Ilioara Oniga ◽

Cristina Pușcaș ◽

Radu Silaghi-Dumitrescu ◽

Neli-Kinga Olah ◽

Bogdan Sevastre ◽

...

Keyword(s):

Antimicrobial Activity ◽

Salmonella Enteritidis ◽

Biological Properties ◽

Gamma Glutamyl Transferase ◽

Reducing Ability ◽

Biological Studies ◽

Antioxidant And Antimicrobial Activity ◽

Agar Well Diffusion Assay ◽

New Research ◽

Glutamyl Transferase

The biological properties and main phenolic compounds of the O. vulgare L. ssp. vulgare extract are described in the present paper. The polyphenolic compounds were analyzed by chromatographic and spectrophotometric techniques. The antioxidant potential was evaluated using several methods: CUPRAC (cupric ion reducing antioxidant capacity), FRAP (ferric reducing ability of plasma), inhibition of lipid peroxidation catalyzed by cytochrome c, and superoxide (SO) scavenging assays. The antimicrobial activity of the oregano extract was evaluated by means of agar-well diffusion assay. The hepatoprotective effect of the O. vulgare extract on CCl4-induced hepatotoxicity was evaluated in rats. Liver injury was estimated by determination of alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), gamma-glutamyl transferase GGT, total protein and albumin concentrations, glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MDA). These values were improved by the administration of oregano extract. A specific phenolic profile was evidenced by these data, with large amounts of rosmarinic and chlorogenic acids. The oregano extract showed very strong antioxidant activity in good agreement with the phenolic content. Antimicrobial activity was good, especially against Salmonella enteritidis and Aspergillus niger strains. The high hepatoprotective, antioxidant and antimicrobial activity, along with polyphenol-rich content, can support the use of O. vulgare in therapy. We also expect our results to open new research directions for designing important new drug products, using indigenous plant material.

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