UV Curable Coatings Based on Urethane Acrylates Containing Eugenol and Evaluation of Their Antimicrobial Activity

This work presents studies on the obtaining of UV-curable coatings with antimicrobial activity. Urethane acrylates (UAs) have excellent physicochemical properties including high reactivity in systems with radical photoinitiators in the presence of UV radiation and good balance between hardness and flexibility in the formed coatings. At the same time, eugenol is well known as the compound hindering the growth of various microorganisms. Hence, the materials obtained by the modification of UA resins with eugenol can be used to protect various surfaces, especially against microorganisms. This study aimed to examine the influence of the amount of eugenol on the chemical, physical, thermal, and mechanical properties of the obtained UA coatings and find the conditions at which the optimal properties for industrial applications such coatings can be achieved. These materials were successfully obtained. Taking into account that eugenol is a very cheap reactant, and it can be obtained from natural sources by the simple distillation method, the proposed method combined the good points of obtaining protective coatings by UV curing with the utilization of vegetable, renewable reactants (biomass), such as components giving special properties to these materials, in this case, antimicrobial properties. In this study, photoreactive coatings with antimicrobial properties for the following microorganisms: fungi (C. albicans), Gram-positive bacteria (S. epidermidis) as well as Gram-negative bacteria (E. coli), were obtained. The obtained coatings were cured over a short time. They were colorless and characterized by a wide range of properties and applications.

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Photoactivated Self-Sanitizing Chlorophyllin-Containing Coatings to Prevent Microbial Contamination in Packaged Food

Coatings ◽

10.3390/coatings8090328 ◽

2018 ◽

Vol 8 (9) ◽

pp. 328 ◽

Cited By ~ 2

Author(s):

Gracia López-Carballo ◽

Pilar Hernández-Muñoz ◽

Rafael Gavara

Keyword(s):

Antimicrobial Activity ◽

High Efficiency ◽

Antimicrobial Properties ◽

Methyl Cellulose ◽

Log Reduction ◽

Gram Positive Bacteria ◽

Sodium Magnesium ◽

Pet Film ◽

Packaged Food ◽

Sodium Copper Chlorophyllin

Chlorophyllins are semi-synthetic porphyrins obtained from chlorophyll that—when exposed to visible light—generate radical oxygen substances with antimicrobial activity. In this work, chlorophyllins incorporated with polyethylene (PE), polyvinyl alcohol (PVOH), (hydroxypropyl)methyl cellulose (HPMC), and gelatin (G) were formulated for application as coatings in packages providing antimicrobial activity after photoactivation. First, the antimicrobial properties of two porphyrins (sodium magnesium chlorophyllin, E-140, and sodium copper chlorophyllin, E-141) were analyzed against L. monocytogenes and Escherichia coli. The results indicated that E-140 was more active than E-141 and that chlorophyllins were more effective against Gram-positive bacteria. In addition, both chlorophyllins were more efficient when irradiated with halogen lamps than with LEDs, and they were inactive in dark conditions. Then, coatings on polyethylene terephthalate (PET) film were prepared, and their effect against the test bacteria was similar to that shown previously with pure chlorophyllins, i.e., greater activity in films containing E-140. Among the coating matrices, those based on PE presented the least effect (1 log reduction), whereas PVOH, HPMC, and G were lethal (7 log reduction). The self-sanitizing effect of these coatings was also analyzed by contaminating the surface of the coatings and irradiating them through the PET surface, which showed high efficiency, although the activity of the coatings was limited to L. monocytogenes. Finally, coated films were applied as separators of bologna slices. After irradiation, all the films showed count reductions of L. monocytogenes and the usual microbial load; the gelatin coating was the most effective, with an average of 3 log reduction.

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Chitosan based antimicrobial films for food packaging applications

e-Polymers ◽

10.1515/epoly.2008.8.1.1082 ◽

2008 ◽

Vol 8 (1) ◽

Cited By ~ 28

Author(s):

Shipra Tripathi ◽

G. K. Mehrotra ◽

P. K. Dutta

Keyword(s):

Antimicrobial Activity ◽

Food Packaging ◽

Antimicrobial Properties ◽

Food Preservation ◽

Pathogenic Microorganisms ◽

Packaging System ◽

Antimicrobial Packaging ◽

Wide Range ◽

Chitosan Films ◽

Antimicrobial Films

AbstractAntimicrobial packaging is one of the most promising active packaging systems. Antimicrobial packaging is the packaging system that is able to kill or inhibit spoilage and pathogenic microorganisms that are contaminating foods. A tremendous effort has been made over the last decade to develop and test films with antimicrobial properties to improve food safety and shelf life. For food preservation, chitosan films are very effective. Chitosan has widely been used in antimicrobial films, to provide edible protective coating, dipping and spraying for the food products due to its antimicrobial properties. Chitosan can be formed into fibers, films, gels, sponges, beads or nanoparticles. Chitosan films have been used as a packaging material for the quality preservation of a variety of food. Chitosan has great potential for a wide range of applications due to its biodegradability, biocompatibility, antimicrobial activity, non-toxicity and versatile chemical and physical properties. The present review outlines the preparation and antimicrobial activity of chitosan based films.

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Antimicrobial Properties of Composites of Chitosan-Silver Doped Zeolites

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2020.18574 ◽

2020 ◽

Vol 20 (10) ◽

pp. 6295-6304

Author(s):

Fabio A. P. Scacchetti ◽

Filipa Fernandes ◽

Artur Ribeiro ◽

Artur Cavaco-Paulo ◽

Joaquim O. Carneiro ◽

...

Keyword(s):

Antimicrobial Activity ◽

Sodium Tripolyphosphate ◽

Antimicrobial Properties ◽

X Ray Diffraction ◽

Gram Negative ◽

X Ray ◽

Gram Positive Bacteria ◽

Ph Conditions ◽

Average Size ◽

Properties Of Composites

The antimicrobial functionality of composites constituted by chitosan with silver-doped zeolites was developed and characterized. A composite with chitosan particles and silver-doped zeolites was synthesized using an ionic gelation process with sodium tripolyphosphate. The chitosan silver-doped zeolites composite obtained presented sizes up to 5 μm, while the silver-doped zeolites had an average size between 0.5 μm and 3.3 μm. The synthesized chitosan silver-doped zeolites composites, as well as the silver-doped zeolites and the chitosan dissolution, were characterized through X-ray diffraction, Fourier Transform Infrared spectroscopy and scanning electron microscopy. The electro kinetic behaviour of chitosan, silver-doped zeolites and chitosan silver-doped zeolites composite was evaluated under different pH conditions. The antimicrobial activity of the composites was evaluated in terms of minimum inhibitory concentrations and minimum lethal concentrations and the results suggest that the chitosan silver-doped zeolites composites show antimicrobial activity against gram-negative and gram-positive bacteria, Escherichia coli and Staphylococcus aureus, respectively and against Candida albicans. The results here presented support the potential application of the composite of chitosan with silver-doped zeolites in the functionalization of textiles with antimicrobial properties.

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ANTIMICROBIAL ACTIVITY AND MINIMUM EFFECTIVE CONCENTRATIONS OF CHEMICAL COMPOUNDS FOUND IN DISINFECTANTS

Hygiene and Sanitation ◽

10.18821/0016-9900-2019-98-10-1031-1036 ◽

2019 ◽

Vol 98 (10) ◽

pp. 1031-1036

Author(s):

Nikolay V. Shestopalov ◽

L. S. Fedorova ◽

A. Yu. Skopin

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Properties ◽

Chemical Compounds ◽

Effective Concentration ◽

Human Welfare ◽

Minimum Effective Concentration ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Rights Protection ◽

Consumer Rights

The antimicrobial properties of various chemical compounds are significantly different. These differences are determined as the properties of the chemical compound and the properties of applied microorganisms. To determine the antimicrobial activity and establish the minimum effective concentrations of the most common chemical compounds, the Research Institute for Disinfectology of the Federal Service on Consumer Rights Protection and Human Welfare Supervision Rospotrebnadzor conducted extensive research. The antimicrobial properties of chemical compounds were studied in relation to the main test microorganisms - gram-negative and gram-positive bacteria, fungi, mold, viruses. The results obtained allowed establishing the minimum effective concentration for 9 chemical compounds in relation to 10 test microorganisms.

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Brevinin-1GHd: a novel Hylarana guentheri skin secretion-derived Brevinin-1 type peptide with antimicrobial and anticancer therapeutic potential

Bioscience Reports ◽

10.1042/bsr20200019 ◽

2020 ◽

Vol 40 (5) ◽

Cited By ~ 2

Author(s):

Yangyang Jiang ◽

Yue Wu ◽

Tao Wang ◽

Xiaoling Chen ◽

Mei Zhou ◽

...

Keyword(s):

Therapeutic Potential ◽

Human Cancer ◽

Wide Spectrum ◽

Antimicrobial Properties ◽

Skin Secretion ◽

Anticancer Activities ◽

Peptide Analog ◽

Wide Range ◽

Low Cytotoxicity ◽

Short Time

Abstract Host-defense antimicrobial peptides (AMPs) from amphibians are usually considered as one of the most promising next-generation antibiotics because of their excellent antimicrobial properties and low cytotoxicity. In the present study, one novel Brevinin-1 type peptide, Brevinin-1GHd, was isolated and characterized from the skin secretion of the frog, Hylarana guentheri. Brevinin-1GHd was found to possess a wide range of antimicrobial activity through penetrating the bacterial membrane within a short time while showing low hemolysis at bactericidal concentrations, even against the resistant strains. It also inhibited and eradicated biofilms that are thought to be closely related to the rise in resistance. Meanwhile, Brevinin-1GHd exhibited wide-spectrum anti-proliferation activity toward human cancer lines. Taken together, these results indicate that Brevinin-1GHd with its excellent antimicrobial and anticancer activities is a promising candidate for a novel antibiotic agent, and study of its structure–activity relationships also provided a rational template for further research and peptide analog design.

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Antibiogram and Phytochemical Analysis of Cinnamon, Clove, and Sichuan Pepper Extracts

Nepal Journal of Biotechnology ◽

10.3126/njb.v9i1.38644 ◽

2021 ◽

Vol 9 (1) ◽

pp. 1-7

Author(s):

Bibek Adhikari ◽

Pradeep Kumar Shah ◽

Roman Karki

Keyword(s):

Escherichia Coli ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Proteus Mirabilis ◽

Antimicrobial Properties ◽

Antimicrobial Activities ◽

Soxhlet Apparatus ◽

Medicinal Plant Extracts ◽

Wide Range

A wide range of medicinal plant extracts has phytochemicals that possess antimicrobial properties and these plants are used to treat several infections. The study aimed to assess the antimicrobial activities of some spices extracts and to evaluate the phytochemicals present in them. The extracts of spices were prepared using Soxhlet apparatus refluxing with methanol and ethanol. The well diffusion technique was implemented for the evaluation of antimicrobial activities of the extracts and the zone of inhibitions was recorded in millimeters. The antimicrobial test was done against five bacterial isolates: Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enterica serotype Typhi, and Staphylococcus aureus and a fungal isolate: Candida albicans. The extracts were concentrated by Rotary Vacuum Evaporator and a stock solution of 200 mg/mL was prepared by dissolving in 10 % DMSO. Concentrations of 40, 60, 80 and 100 mg/mL extracts were used for antimicrobial activity. The result of this study showed that clove extracts had the highest antimicrobial property against all the test microorganisms. Methanolic extract of clove had the highest inhibitory effect against Proteus mirabilis (24.21±0.15 mm), Pseudomonas aeruginosa (19.78±0.23 mm), and Candida albicans (20.07±0.08 mm) whereas ethanolic extract was effective against Escherichia coli (20.44±0.16 mm), Salmonella Typhi (21.66±0.31 mm) and Candida albicans (21.11±0.09 mm). Cinnamon and pepper extracts, leaving some exceptions, also had antimicrobial properties. The presence of phytochemicals: polyphenols, flavonoids, and tannins are the major components responsible for antimicrobial activity. Thereby, this study successfully demonstrated the possibilities of using spices extracts in the treatment of microbial infections.

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Chemical Composition and Antimicrobial Activity of Teucrium Capitatum L. Subsp. Lusitanicum (Schreb.) T. Navarro & Rosua Essential Oil

Journal of Chromatographic Science ◽

10.1093/chromsci/bmaa086 ◽

2020 ◽

Author(s):

Haïfa Debbabi ◽

Ridha El Mokni ◽

Ibrahim Jlassi ◽

Rajesh K Joshi ◽

Saoussen Hammami

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Antimicrobial Properties ◽

Significant Activity ◽

Bacterial Strains ◽

Gram Positive Bacteria ◽

Sesquiterpene Hydrocarbons ◽

Germacrene D

Abstract Chemical composition and antimicrobial activity of Teucrium capitatum L. subsp. lusitanicum essential oil was investigated for the first time in the present study. Qualitative and quantitative analyses of the chemical composition by gas chromatography and mass spectrometry (GC–FID and GC–MS) revealed the presence of 60 compounds representing 97.6% of the whole constituents. The main compounds were germacrene D (47.1%), spathulenol (5.8%), α-selinene (5.3%), germacrene A (2.9%), δ-cadinene (2.8%) and cubenol (2.7%). In vitro, the antimicrobial activity was investigated against five bacterial strains along with the yeast Candida albicans using broth microdilution assay. T. capitatum subsp. lusitanicum essential oil showed significant activity against the gram-positive bacteria Staphylococcus aureus (MIC = MBC = 78 μg mL−1), Bacillus subtilis (MIC = MBC = 156 μg mL−1) and the yeast C. albicans (MIC = MFC = 156 μg mL−1). The great potential of antimicrobial effects is most likely due to the very high percentage of sesquiterpene hydrocarbons particularly to germacrene D, for which the antimicrobial properties have been previously reported.

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Studies on the Antimicrobial Activity of Juglans regia

The American Journal of Chinese Medicine ◽

10.1142/s0192415x97000202 ◽

1997 ◽

Vol 25 (02) ◽

pp. 175-180 ◽

Cited By ~ 31

Author(s):

Abdulaziz M. Alkhawajah

Keyword(s):

Antimicrobial Activity ◽

Juglans Regia ◽

Dependent Manner ◽

Pathogenic Yeast ◽

Gram Positive Bacteria ◽

Additive Action ◽

Esherichia Coli ◽

Wide Range ◽

Micro Organisms ◽

Dose Dependent

Juglans regia L. bark is used in some countries as a toothbrush and as a dye for coloring the lips for cosmetic purposes. Its extract showed a broad spectrum antimicrobial activity in a dose dependent manner. It inhibited the growth of several species of pathogenic micro-organisms representing Gram-positive bacteria (Staphylococcus aureus and Streptococcus mutans), Gram-negative bacteria (Esherichia coli and Pseudomonas aeruginosa) and a pathogenic yeast (Candida albicans). The extract has either synergistic or additive action when tested with a wide range of antibacterial drugs. It also increased the pH of saliva. Thus, brushing the teeth with this bark may improve oral hygiene, prevent plaque and caries formation, and reduce the incidence of gingival and periodontal infections.

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FORMULATION, CHARACTERIZATION AND DETERMINATION OF ANTIBACTERIAL ACTIVITY OF POMEGRANATE (Punicagranatum) GEL.

Journal of Applied Pharmacy ◽

10.21065/19204159.7.13 ◽

2015 ◽

Vol 7 ◽

pp. 13

Author(s):

Afendi Dahlan ◽

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Antibacterial Activity ◽

Antimicrobial Properties ◽

Pathogenic Microorganisms ◽

Wide Range ◽

Common Skin

revious studies have shown that Punicagranatum (P. granatum) extract exhibited antimicrobial activity against a wide range of pathogenic microorganisms. Thus, the aim of study was to formulate and determine the effectiveness of antimicrobial properties of gel containing P. granatummethanol extract against selected common skin pathogens including Staphylococcus aureus, Staphylococcusepidermidis, Pseudomonas aeruginosaand Candidaalbicans.

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Antimicrobial Properties of Water-Soluble Polysaccharides and Alcoholic Extracts of Laetiporus Sulphureus (Bull.) Murrill Mycelium and Development of Biotechnology for Its Production in Immobilized Culture on Bacterial Cellulose

Antibiotics and Chemotherapy ◽

10.37489/0235-2990-2020-65-1-2-10-14 ◽

2020 ◽

Vol 65 (1-2) ◽

pp. 10-14

Author(s):

I. A. Gavryushina ◽

T. I. Gromovykh ◽

N. B. Feldman ◽

S. V. Lutsenko ◽

V. I. Ponomarenko ◽

...

Keyword(s):

Antimicrobial Activity ◽

Bacterial Cellulose ◽

Synthetic Medium ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Water Soluble ◽

Biologically Active ◽

Aqueous Extracts ◽

Gram Positive Bacteria ◽

Immobilized Culture

The article discusses the development of a new method of producing Laetiporus sulphureus (Bull.) Murrill basidiomycete mycelium immobilized on a bacterial cellulose matrix. Mycelium contains biologically active compounds with antimicrobial activity against Gram-positive bacteria, including resistant staphylococcus. The aim of the work was to obtain immobilized mycelium by co-cultivation of L.sulphureus with the producer of bacterial cellulose Gluconacetobacter hansenii. The authors found that when co-culturing the basidial L.sulphureus strain with the bacterial cellulose producing G.hansenii strain, productivity increases by 3.2 times on H5/1 synthetic medium and by 1.9 times on natural Maltax-10 medium (concentration 5%). The resulting immobilized L.sulphureus mycelium has antibacterial properties; its aqueous extracts contain glucans.

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