scholarly journals Diversity of Pod Shape in Pisum

Diversity ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 203
Author(s):  
Thomas Henry Noel Ellis ◽  
Julie M. I. Hofer ◽  
Eleni Vikeli ◽  
Michael J. Ambrose ◽  
Paola Higuera-Poveda ◽  
...  
Keyword(s):  
Allelic Variation ◽  
Size Variation ◽  
Width Ratio ◽  
Negative Regulators ◽  
Wide Range ◽  
Yield Determination ◽  
Organ Specific ◽  
Length Width ◽  
End Use ◽  
Pod Length

The seed-containing pod is the defining structure of plants in the legume family, yet pods exhibit a wide range of morphological variation. Within a species pod characters are likely to be correlated with reproductive strategy, and within cultivated forms will correspond to aspects of yield determination and/or end use. Here variation in pod size, described as pod length: pod width ratio, has been analyzed in pea germplasm represented by 597 accessions. This pod size variation is discussed with respect to population structure and to known classical pod morphology mutants. Variability of the pod length: width ratio can be explained by allelic variation at two genetic loci that may correspond to organ-specific negative regulators of growth.

scholarly journals Standing genetic variation in a tissue-specific enhancer underlies selfing-syndrome evolution inCapsella

2016 ◽  
Vol 113 (48) ◽  
pp. 13911-13916 ◽  
Author(s):  
Adrien Sicard ◽  
Christian Kappel ◽  
Young Wha Lee ◽  
Natalia Joanna Woźniak ◽  
Cindy Marona ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Mating System ◽  
Allelic Variation ◽  
Size Variation ◽  
Regulatory Elements ◽  
Flower Size ◽  
Evolutionary Transitions ◽  
Tissue Specific ◽  
Organ Specific ◽  
Petal Size

Mating system shifts recurrently drive specific changes in organ dimensions. The shift in mating system from out-breeding to selfing is one of the most frequent evolutionary transitions in flowering plants and is often associated with an organ-specific reduction in flower size. However, the evolutionary paths along which polygenic traits, such as size, evolve are poorly understood. In particular, it is unclear how natural selection can specifically modulate the size of one organ despite the pleiotropic action of most known growth regulators. Here, we demonstrate that allelic variation in the intron of a general growth regulator contributed to the specific reduction of petal size after the transition to selfing in the genusCapsella. Variation within this intron affects an organ-specific enhancer that regulates the level of STERILE APETALA (SAP) protein in the developing petals. The resulting decrease inSAPactivity leads to a shortening of the cell proliferation period and reduced number of petal cells. The absence of private polymorphisms at the causal region in the selfing species suggests that the small-petal allele was captured from standing genetic variation in the ancestral out-crossing population. Petal-size variation in the current out-crossing population indicates that several small-effect mutations have contributed to reduce petal-size. These data demonstrate how tissue-specific regulatory elements in pleiotropic genes contribute to organ-specific evolution. In addition, they provide a plausible evolutionary explanation for the rapid evolution of flower size after the out-breeding-to-selfing transition based on additive effects of segregating alleles.

scholarly journals Image-based phenotyping for identification of QTL determining fruit shape and size in American cranberry (Vaccinium macrocarponL.)

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5461 ◽  
Author(s):  
Luis Diaz-Garcia ◽  
Giovanny Covarrubias-Pazaran ◽  
Brandon Schlautman ◽  
Edward Grygleski ◽  
Juan Zalapa
Keyword(s):  
Persistent Homology ◽  
Fruit Size ◽  
Fruit Shape ◽  
Quality Parameters ◽  
Width Ratio ◽  
Size And Shape ◽  
American Cranberry ◽  
Length Width ◽  
Fruit Size And Shape ◽  
End Use

Image-based phenotyping methodologies are powerful tools to determine quality parameters for fruit breeders and processors. The fruit size and shape of American cranberry (Vaccinium macrocarponL.) are particularly important characteristics that determine the harvests’ processing value and potential end-use products (e.g., juice vs. sweetened dried cranberries). However, cranberry fruit size and shape attributes can be difficult and time consuming for breeders and processors to measure, especially when relying on manual measurements and visual ratings. Therefore, in this study, we implemented image-based phenotyping techniques for gathering data regarding basic cranberry fruit parameters such as length, width, length-to-width ratio, and eccentricity. Additionally, we applied a persistent homology algorithm to better characterize complex shape parameters. Using this high-throughput artificial vision approach, we characterized fruit from 351 progeny from a full-sib cranberry population over three field seasons. Using a covariate analysis to maximize the identification of well-supported quantitative trait loci (QTL), we found 252 single QTL in a 3-year period for cranberry fruit size and shape descriptors from which 20% were consistently found in all years. The present study highlights the potential for the identified QTL and the image-based methods to serve as a basis for future explorations of the genetic architecture of fruit size and shape in cranberry and other fruit crops.

Strain concentrations at the corners of stretched rubber sheets

1971 ◽  
Vol 6 (4) ◽  
pp. 279-285 ◽  
Author(s):  
P B Lindley
Keyword(s):  
Experimental Investigation ◽  
Relative Size ◽  
Width Ratio ◽  
Cube Root ◽  
Strain Concentration ◽  
Wide Range ◽  
Nominal Strain ◽  
Length Width ◽  
Concentration Factors ◽  
Better Than

A numerical and experimental investigation has been made of the strain-concentration factors K at the corners of stretched rectangular rubber sheets having a wide range of length/width ratio, for corner curves of various shapes and sizes. It was found that K is approximately inversely proportional to the cube root of the relative size of the curve, and that elliptic corner curves are better than sine curves. Undercutting is deleterious, but corner wedges with angles of less than 45° have zero strain at the corner. K generally decreases with increasing nominal strain.

scholarly journals Microsatellite and Chromosome Evolution of Parthenogenetic Sitobion Aphids in Australia

Genetics ◽  
1996 ◽  
Vol 144 (2) ◽  
pp. 747-756 ◽  
Author(s):  
Paul Sunnucks ◽  
Phillip R England ◽  
Andrea C Taylor ◽  
Dinah F Hales
Keyword(s):  
Sexual Reproduction ◽  
Chromosome Evolution ◽  
Genetic Recombination ◽  
Allelic Variation ◽  
Repeat Unit ◽  
Chromosomal Evolution ◽  
Microsatellite Variation ◽  
Wide Range ◽  
Sexual Recombination ◽  
Minimum Numbers

Abstract Single-locus microsatellite variation correlated perfectly with chromosome number in Sitobion miscanthi aphids. The microsatellites were highly heterozygous, with up to 10 alleles per locus in this species. Despite this considerable allelic variation, only seven different S. miscanthi genotypes were discovered in 555 individuals collected from a wide range of locations, hosts and sampling periods. Relatedness between genotypes suggests only two successful colonizations of Australia. There was no evidence for genetic recombination in 555 S. miscanthi so the occurrence of recent sexual reproduction must be near zero. Thus diversification is by mutation and chromosomal rearrangement alone. Since the aphids showed no sexual recombination, microsatellites can mutate without meiosis. Five of seven microsatellite differences were a single repeat unit, and one larger jump is likely. The minimum numbers of changes between karyotypes corresponded roughly one-to-one with microsatellite allele changes, which suggests very rapid chromosomal evolution. A chromosomal fission occurred in a cultured line, and a previously unknown chromosomal race was detected. All 121 diverse S. near fragariae were heterozygous but revealed only one genotype. This species too must have a low rate of sexual reproduction and few colonizations of Australia.

scholarly journals Synonymous single nucleotide polymorphism in arsenic (+3) methyltransferase of the Western mosquitofish (Gambusia affinis) and its gene expression among field populations

Ecotoxicology ◽  
2021 ◽  
Author(s):  
Daesik Park ◽  
Catherine R. Propper ◽  
Guangning Wang ◽  
Matthew C. Salanga
Keyword(s):  
Gene Expression ◽  
Sequence Variation ◽  
Allelic Variation ◽  
Arsenic Concentration ◽  
Gambusia Affinis ◽  
Contamination Level ◽  
Water Medium ◽  
Single Nucleotide ◽  
Wide Range ◽  
High Arsenic

AbstractNaturally occurring arsenic is toxic at extremely low concentrations, yet some species persist even in high arsenic environments. We wanted to test if these species show evidence of evolution associated with arsenic exposure. To do this, we compared allelic variation across 872 coding nucleotides of arsenic (+3) methyltransferase (as3mt) and whole fish as3mt gene expression from three field populations of Gambusia affinis, from water sources containing low (1.9 ppb), medium-low (3.3 ppb), and high (15.7 ppb) levels of arsenic. The high arsenic site exceeds the US EPA’s Maximum Contamination Level for drinking water. Medium-low and high populations exhibited homozygosity, and no sequence variation across all animals sampled. Eleven of 24 fish examined (45.8%) in the low arsenic population harbored synonymous single nucleotide polymorphisms (SNPs) in exons 4 and/or 10. SNP presence in the low arsenic population was not associated with differences in as3mt transcript levels compared to fish from the medium-low site, where SNPs were noted; however, as3mt expression in fish from the high arsenic concentration site was significantly lower than the other two sites. Low sequence variation in fish populations from sites with medium-low and high arsenic concentrations suggests greater selective pressure on this allele, while higher variation in the low population suggests a relaxed selection. Our results suggest gene regulation associated with arsenic detoxification may play a more crucial role in influencing responses to arsenic than polymorphic gene sequence. Understanding microevolutionary processes to various contaminants require the evaluation of multiple populations across a wide range of pollution exposures.

scholarly journals Sequential Oxidation on Wood and Its Application in Pb2+ Removal from Contaminated Water

2021 ◽  
Vol 2 (2) ◽  
pp. 245-256
Author(s):  
Priyanka R. Sharma ◽  
Sunil K. Sharma ◽  
Marc Nolan ◽  
Wenqi Li ◽  
Lakshta Kundal ◽  
...  
Keyword(s):  
Adsorption Capacity ◽  
Average Length ◽  
Sodium Chlorite ◽  
Contaminated Water ◽  
Maximum Adsorption Capacity ◽  
Fiber Morphology ◽  
Carboxylate Groups ◽  
Carboxylate Content ◽  
Wide Range ◽  
Length Width

Raw wood was subjected to sequential oxidation to produce 2,3,6-tricarboxycellulose (TCC) nanofibers with a high surficial charge of 1.14 mmol/g in the form of carboxylate groups. Three oxidation steps, including nitro-oxidation, periodate, and sodium chlorite oxidation, were successfully applied to generate TCC nanofibers from raw wood. The morphology of extracted TCC nanofibers measured using TEM and AFM indicated the average length, width, and thickness were in the range of 750 ± 110, 4.5 ± 1.8, and 1.23 nm, respectively. Due to high negative surficial charges on TCC, it was studied for its absorption capabilities against Pb2+ ions. The remediation results indicated that a low concentration of TCC nanofibers (0.02 wt%) was able to remove a wide range of Pb2+ ion impurities from 5–250 ppm with an efficiency between 709–99%, whereby the maximum adsorption capacity (Qm) was 1569 mg/g with R2 0.69531 calculated from Langmuir fitting. It was observed that the high adsorption capacity of TCC nanofibers was due to the collective effect of adsorption and precipitation confirmed by the FTIR and SEM/EDS analysis. The high carboxylate content and fiber morphology of TCC has enabled it as an excellent substrate to remove Pb2+ ions impurities.

scholarly journals Evaluating milling and baking quality associated with a Fusarium head blight resistance-enhancing genome deletion in wheat

2021 ◽  
Author(s):  
David F. Garvin ◽  
Linda Dykes
Keyword(s):  
Fusarium Head Blight ◽  
Spring Wheat ◽  
Fusarium Head Blight Resistance ◽  
Seed Storage ◽  
Isogenic Line ◽  
Head Blight ◽  
Hard Red Spring Wheat ◽  
Baking Quality ◽  
Wide Range ◽  
End Use

AbstractWheat (Triticum aestivum L.) breeding involves improvement of a wide range of traits. However, selection for these traits is only acceptable if the end use quality of the wheat is not compromised. In hard red spring wheat, the predominant end use of flour is bread. In this study, milling and baking quality characteristics were compared in the hard red spring wheat ‘Apogee’ and a near-isogenic line of Apogee (‘A30’) that contains a spontaneous segmental deletion of the long arm of chromosome arm 3DL that is associated with enhanced resistance to Fusarium head blight caused by the fungal pathogen Fusarium graminearum (Schwabe). Apogee and A30 were grown together in replicated greenhouse experiments, and the resultant grain was used to compare a diverse spectrum of grain characteristics and milling and baking properties of the grain in the two wheat genotypes. The major difference detected was a significant increase in protein content in A30, which had nearly 21% more flour protein than Apogee. This difference did not affect any of the flour properties or baking characteristics evaluated, suggesting that the increased protein concentrations in A30 are not associated with the principal seed storage properties associated with baking quality. These results indicate that despite the size of the deletion in A30, no key genes associated with end use quality are located on that chromosome segment. The deletion may therefore find use in efforts to enhance Fusarium head blight in hard red spring wheat.

scholarly journals A Novel GIS-Based Approach for Automated Detection of Nearshore Sandbar Morphological Characteristics in Optical Satellite Imagery

2021 ◽  
Vol 13 (11) ◽  
pp. 2233
Author(s):  
Rasa Janušaitė ◽  
Laurynas Jukna ◽  
Darius Jarmalavičius ◽  
Donatas Pupienis ◽  
Gintautas Žilinskas
Keyword(s):  
Satellite Imagery ◽  
Morphological Characteristics ◽  
Unsupervised Classification ◽  
Landsat 8 ◽  
Multi Scale ◽  
Medium Resolution ◽  
Wide Range ◽  
Position Index ◽  
Local Relief ◽  
Length Width

Satellite remote sensing is a valuable tool for coastal management, enabling the possibility to repeatedly observe nearshore sandbars. However, a lack of methodological approaches for sandbar detection prevents the wider use of satellite data in sandbar studies. In this paper, a novel fully automated approach to extract nearshore sandbars in high–medium-resolution satellite imagery using a GIS-based algorithm is proposed. The method is composed of a multi-step workflow providing a wide range of data with morphological nearshore characteristics, which include nearshore local relief, extracted sandbars, their crests and shoreline. The proposed processing chain involves a combination of spectral indices, ISODATA unsupervised classification, multi-scale Relative Bathymetric Position Index (RBPI), criteria-based selection operations, spatial statistics and filtering. The algorithm has been tested with 145 dates of PlanetScope and RapidEye imagery using a case study of the complex multiple sandbar system on the Curonian Spit coast, Baltic Sea. The comparison of results against 4 years of in situ bathymetric surveys shows a strong agreement between measured and derived sandbar crest positions (R2 = 0.999 and 0.997) with an average RMSE of 5.8 and 7 m for PlanetScope and RapidEye sensors, respectively. The accuracy of the proposed approach implies its feasibility to study inter-annual and seasonal sandbar behaviour and short-term changes related to high-impact events. Algorithm-provided outputs enable the possibility to evaluate a range of sandbar characteristics such as distance from shoreline, length, width, count or shape at a relevant spatiotemporal scale. The design of the method determines its compatibility with most sandbar morphologies and suitability to other sandy nearshores. Tests of the described technique with Sentinel-2 MSI and Landsat-8 OLI data show that it can be applied to publicly available medium resolution satellite imagery of other sensors.

Meshing Method of High Precision FEM in Large Complex Structural Simulations

2012 ◽  
Vol 195-196 ◽  
pp. 701-704
Author(s):  
Yan Hua Xue ◽  
Zhi Guang Wang ◽  
Xiao Hong Li ◽  
Xin Jiang
Keyword(s):  
Finite Element ◽  
Complex Structure ◽  
Width Ratio ◽  
Two Dimensional ◽  
Element Analysis ◽  
Large Complex ◽  
Mesh Density ◽  
Length Width ◽  
Complex Structural ◽  
Dimensional Element

Shing is playing an important role in the large complex structural FEM simulations; it has a direct effect on calculating precision of structural simulations. For increasing the calculation accuracy and analysis accuracy of complex structure, the finite element meshing problems is proposed on the finite element analysis of large complicated structures. The effects caused by element type, mesh density and intergradations on calculating precision are studied and discussed. A research argues that with length-width ratio of 1~2 and length-thickness ration of 1.5~4.5 of two-dimensional rectangular element, the quality of meshing method of two-dimensional element is above normal. As the height of one-dimensional element is equal to the sum of reinforcing rib height of outer panel and half the thickness of panel, more accurate results can be obtained.

scholarly journals A Transcriptomic Approach to the Metabolism of Tetrapyrrolic Photosensitizers in a Marine Annelid

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3924
Author(s):  
Maria Leonor Santos ◽  
Mariaelena D’Ambrosio ◽  
Ana P. Rodrigo ◽  
A. Jorge Parola ◽  
Pedro M. Costa
Keyword(s):  
Metabolic Pathways ◽  
Molecular Networks ◽  
Bile Pigments ◽  
Rna Seq ◽  
The Past ◽  
Wide Range ◽  
Genes Encoding ◽  
Organ Specific ◽  
Bright Green ◽  
Green Coloration

The past decade has seen growing interest in marine natural pigments for biotechnological applications. One of the most abundant classes of biological pigments is the tetrapyrroles, which are prized targets due their photodynamic properties; porphyrins are the best known examples of this group. Many animal porphyrinoids and other tetrapyrroles are produced through heme metabolic pathways, the best known of which are the bile pigments biliverdin and bilirubin. Eulalia is a marine Polychaeta characterized by its bright green coloration resulting from a remarkably wide range of greenish and yellowish tetrapyrroles, some of which have promising photodynamic properties. The present study combined metabolomics based on HPLC-DAD with RNA-seq transcriptomics to investigate the molecular pathways of porphyrinoid metabolism by comparing the worm’s proboscis and epidermis, which display distinct pigmentation patterns. The results showed that pigments are endogenous and seemingly heme-derived. The worm possesses homologs in both organs for genes encoding enzymes involved in heme metabolism such as ALAD, FECH, UROS, and PPOX. However, the findings also indicate that variants of the canonical enzymes of the heme biosynthesis pathway can be species- and organ-specific. These differences between molecular networks contribute to explain not only the differential pigmentation patterns between organs, but also the worm’s variety of novel endogenous tetrapyrrolic compounds.

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