scholarly journals Exploration of the Potential for Efficient Fiber Degradation by Intestinal Microorganisms in Diqing Tibetan Pigs

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 275
Author(s):  
Lijie Yang ◽  
Gang Wang ◽  
Junyan Zhou ◽  
Yuting Yang ◽  
Hongbin Pan ◽  
...  
Keyword(s):  
Dietary Fiber ◽  
Degradation Efficiency ◽  
Neutral Detergent Fiber ◽  
Crude Fiber ◽  
Microbial Composition ◽  
Intestinal Microorganisms ◽  
Tibetan Pig ◽  
Fiber Degradation ◽  
Tibetan Pigs ◽  
Significant Difference

In order to study the potential for efficient fiber degradation by intestinal microorganisms in Diqing Tibetan pigs, we first investigated the dietary structure of Diqing Tibetan pigs in their original habitat, then 60 healthy adult Diqing Tibetan pigs were randomly divided into 2 groups with 6 replicates each and 5 pigs in each replicate. The content of neutral detergent fiber in treatment 1 and 2 were adjusted to 20% and 40%, respectively. The total tract digestibility of nutrients and the degradation efficiency of fecal microorganisms to different types of fiber were determined. Results showed that the composition and nutritional level of Diqing Tibetan pig original diet differed greatly in different seasons. The content of crude fiber in the original diet was as high as 12.3% and the neutral detergent fiber was 32.5% in April, while the content of crude fiber was 4.9% and the neutral detergent fiber was 13.3% in October. With the increase of dietary fiber level, the total tract apparent digestibility of dry matter, crude fiber, crude protein, acid detergent fiber, neutral detergent fiber, ether extract, and organic matter decreased significantly (p < 0.05), and the contents of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, and isovaleric acid in the feces were also significantly (p < 0.05) reduced. The ability of Diqing Tibetan pig fecal microorganisms to degrade neutral detergent fiber was significantly higher (p < 0.05) than “Duroc × Landrace × Yorkshire” pig. In addition, there was no significant difference (p > 0.05) in the degradation efficiency of the same type of fiber between NDF-20 and NDF-40 groups. Our results strongly suggested that Diqing Tibetan pigs have the potential to efficiently utilize fiber, and their unique intestinal microbial composition is the main reason for their efficient utilization of dietary fiber.

scholarly journals Effects of active dry yeast on growth performance, rumen fermentation characteristics and slaughter performance in beef cattle

2019 ◽  
Author(s):  
Siqiang Liu ◽  
Mei Yuan ◽  
Kun Kang ◽  
Zhisheng Wang ◽  
Lizhi Wang ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Relative Abundance ◽  
Average Daily Gain ◽  
Neutral Detergent Fiber ◽  
Carcass Weight ◽  
Control Group ◽  
Microbial Composition ◽  
Significant Difference ◽  
Slaughter Performance ◽  
Active Dry Yeast

AbstractThis study aimed to investigate the effects of active dry yeast (ADY) on rumen microbial composition and slaughter performance of beef cattle. Thirty-two finishing beef cattle (simmental crossbred cattle ♂ × cattle-yaks ♀), with an average body weight of 110 ± 12.85 kg, were randomly assigned to one of four treatments: the low plane of nutrition group (Control), low plane of nutrition group + ADY 2 g/head/d (ADY2), low plane of nutrition group + ADY 4 g/head/d (ADY4) and high plane of nutrition group (HPN). ADY supplementation increased average daily gain (P<0.001), and the carcass weight of ADY4 group had no significant difference with HPN group (P>0.05). The serum glutamic-pyruvic transaminase activity in control and ADY4 group was higher than HPN group (P=0.001). The neutral detergent fiber (P=0.022) and acid detergent fiber (P=0.043) digestibility in HPN group was greater than control, but no difference was obtained among ADY2, ADY4 and HPN group (P>0.05). The rumen ammonium nitrogen content in control was greater than ADY2 and ADY4 group (P=0.003), and no difference was obtained ADY2, ADY4 and HPN group (P>0.05). The propionic acid content in the rumen in ADY2, ADY4, and HPN group were greater than control group (P<0.001). The simpson (P=0.014) and shannon (P=0.045) indexes in control and HPN group were greater than ADY4 group. At the phylum level, the relative abundance of Firmicutes in the HPN group was higher than ADY4 group (P=0.015). At the genus level, HPN and ADY4 were clustered together, and the relative abundance of Ruminococcaceae UCG-002 in ADY4 group was higher than control and HPN group (P=0.004). In conclusion, supplementation ADY 4 g/head/d shift the rumen microbial composition of beef cattle fed low plane of nutrition to a more similar level with cattle fed with HPN diet, produced comparable carcass weight with HPN diet.

scholarly journals Effects of Eubiotic Lignocellulose on the Gut Microbiota and Metabolism of Chickens

2020 ◽  
Author(s):  
Baosheng Sun ◽  
Linyue Hou ◽  
Yu Yang
Keyword(s):  
Blood Glucose ◽  
Gut Microbiota ◽  
Growth Performance ◽  
Dietary Fiber ◽  
Liver Glycogen ◽  
Bacteroides Thetaiotaomicron ◽  
Relative Expression ◽  
Fiber Degradation ◽  
Significant Difference ◽  
Performance Development

Abstract Background: Dietary fiber is an important factor shaping the gut microbiota. Eubiotic lignocellulose is a useful source of dietary fiber for chickens; however, research on its effects on the gut microbiota and metabolism of chickens is limited. Moreover, the gut microbiota regulates the host metabolism, which generally depend on microbiotal metabolites—short-chain fatty acids (SCFAs) and the SCFA receptors; however, related research reports on the SCFA receptors of chickens are lacking. Given this, we added three different levels of eubiotic lignocellulose (0%, 1%, and 2%) to the feed of ISA brown hens (IBH) for 0–8 weeks, with the aim of observing the effects of added eubiotic lignocellulose on the gut microbiota, SCFAs and its receptors and metabolism of chickens. Results: The results showed that the addition of eubiotic lignocellulose showed no significant difference among groups in terms of the growth performance, development of the cecum and microbial diversity of IBH (P > 0.05), but significantly (P < 0.05) increased the relative abundance of the excellent fiber-degradation bacterium Bacteroides thetaiotaomicron, the acetate-producing bacterium Sutterella and fiber digestibility. The addition of 1% eubiotic lignocellulose significantly (P < 0.05) increased the lactate-producing bacterium Lactobacillus panis, the butyrate-producing bacterium Oscillospira and the concentration of SCFAs in the cecum chyme. The addition of eubiotic lignocellulose had no significant effect on the relative expression of the SCFA receptor–G protein-coupled receptor 43 (GPR43) mRNA in the liver and cecum (P > 0.05), resulting in there being no significant difference (P > 0.05) among groups in the metabolism of chickens, including gastrointestinal hormones glucagon-like peptide-1 (GLP-1), peptide tyrosine–tyrosine (PYY), blood glucose and liver glycogen. Conclusions: The addition of eubiotic lignocellulose can increase the abundance of the fiber-degradation bacterium Bacteroides thetaiotaomicron and the fiber digestion of chickens at 8 weeks. The addition of 1% eubiotic lignocellulose is more conducive to the fiber-degradation bacteria and the SCFA-producing bacteria, resulting in increasing of the production of SCFAs. However, because the difference of the concentration of SCFAs was not great among groups, there was no significant change in the growth performance of chickens, the development of the cecum and the relative expression of GPR43 mRNA, resulting in slight effects on the metabolism, including GLP-1 and PYY and the blood glucose and liver glycogen of chickens. Increasing our understanding of this would be beneficial to providing a theoretical basis for the application of eubiotic lignocellulose and improve the growth performance and health of chickens.

scholarly journals Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white Pieris canidia (Lepidoptera: Pieridae)

Life ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 254
Author(s):  
Ying Wang ◽  
Jianqing Zhu ◽  
Jie Fang ◽  
Li Shen ◽  
Shuojia Ma ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
16S Rrna Gene Sequencing ◽  
Adult Survival ◽  
Rrna Gene ◽  
Life Stages ◽  
Microbial Composition ◽  
Significant Difference ◽  
Functional Inference ◽  
Rrna Gene Sequencing ◽  
Insect Fitness

We characterized the gut microbial composition and relative abundance of gut bacteria in the larvae and adults of Pieris canidia by 16S rRNA gene sequencing. The gut microbiota structure was similar across the life stages and sexes. The comparative functional analysis on P. canidia bacterial communities with PICRUSt showed the enrichment of several pathways including those for energy metabolism, immune system, digestive system, xenobiotics biodegradation, transport, cell growth and death. The parameters often used as a proxy of insect fitness (development time, pupation rate, emergence rate, adult survival rate and weight of 5th instars larvae) showed a significant difference between treatment group and untreated group and point to potential fitness advantages with the gut microbiomes in P. canidia. These data provide an overall view of the bacterial community across the life stages and sexes in P. canidia.

scholarly journals Production and nutritional value of the wheat silage managed with different cutting systems

2017 ◽  
Vol 38 (1) ◽  
pp. 335 ◽  
Author(s):  
Rodolfo Carletto ◽  
Mikael Neumann ◽  
Danúbia Nogueira Figueira ◽  
Guilherme Fernando Mattos Leão ◽  
Egon Henrique Horst ◽  
...  
Keyword(s):  
Mass Production ◽  
Block Design ◽  
Neutral Detergent Fiber ◽  
Control Treatment ◽  
Lower Amount ◽  
Mineral Matter ◽  
Winter Cereal ◽  
Winter Cereals ◽  
Cereal Species ◽  
Significant Difference

Winter cereals are mainly used for human and animal nutrition and several studies are now exploring their potential as conserved forage (hay and silage). Among the winter cereal species available for cultivation in the south of Brazil, which is major winter cereals producer in the country, the wheat cv. BRS Umbu has attracted special attention. However, few studies have investigated the potential of this cultivar for silage production. The aim of this study was to evaluate the production and quality of silage from the dual-purpose wheat, BRS Umbu, subjected to different cut managements treatments: T1 - without cut (control), T2 - one cut and T3 - two cuts. Each plot represented an experimental unit in a randomized block design, with 5 replicates per treatment. Two representative samples were collected from each plot to determine morphological segmentation (stem, leaf and ear) and dry matter (DM) content of the whole plant and its morphological components. At the time of opening of the silos, food chemical analysis and pH determination were performed. Forage mass production decreased by 26.88% and 67.82%, respectively, with one and two cuts, compared to control. The DM content of the ensiled plant was 49.9 g kg-1 for the control, 54.7 g kg-1 with one cut and 63.2 g kg-1 with two cuts, at the time of ensiling. Management cuts changed the morphological components of the plants, with a lower proportion of stem (28%) in plants subjected to two cuts. The control treatment showed fewer leaves in ensiled plant (9.6%) and intermediate amount of stalk (52.8%), and was significantly different (p < 0.05) to treatment of a cut. Regarding feed chemistry evaluations, no significant difference (p > 0.05) was observed for mineral matter (MM), crude protein (CP) and hemicellulose (HEM) between the different cutting regimens. However, neutral detergent fiber (NDF) and acid detergent fiber (ADF) decreased (p < 0.05) as the number of cuts increased. The control treatment showed higher NDF and ADF content (563.2 and 357.9 g kg-1 DM, respectively) and lower amount of total digestible nutrients (TDN) and net energy of lactation (NEL). However, the estimated milk production (EMP) was superior for this treatment (22,447 l ha-1), demonstrating the high impact of reduced forage mass production with increased number of cuts.

scholarly journals Diet components as internal indicators in the determination of the apparent digestibility coefficients for Nile tilapia

2017 ◽  
Vol 52 (6) ◽  
pp. 393-400
Author(s):  
Rafael Lopes da Silva ◽  
Eric Portilho de Araújo ◽  
Mariucha Karina Honório Ribeiro Rocha ◽  
Flavia Mota Damasceno ◽  
Jakeline Marcela Azambuja de Freitas ◽  
...  
Keyword(s):  
Nile Tilapia ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
Crude Fiber ◽  
Animal Origin ◽  
Apparent Digestibility ◽  
Plant Origin ◽  
Acid Detergent Fiber ◽  
Dunnett's Test

Abstract: The objective of this work was to evaluate the diet components - crude fiber, neutral detergent fiber, acid detergent fiber, hemicellulose, cellulose, and lignin - as internal indicators in the determination of the apparent digestibility coefficients of dry matter, gross energy, and of the nutrients - crude protein, phosphorus, and amino acids - in Nile tilapia (Oreochromis niloticus). Groups of ten juveniles (80.3±1 g) were randomly distributed in six tanks of 250 L and fed two practical diets, either of a plant-origin diet or of a plant- and animal-origin diet. Both diets were supplemented with 0.1% chromium (III) oxide (Cr2O3). Faeces were collected by the modified Guelph system. The apparent digestibility coefficients were determined by the content difference of the internal indicators, present in the diets and faeces, and compared by Dunnett’s test to those obtained by the use of Cr2O3. Cellulose was effective in the determination of the apparent digestibility of dry matter, energy, and nutrients of both experimental diets; and the acid detergent fiber was effective only for the diet composed exclusively of plant-origin ingredients. The use of crude fiber, neutral detergent fiber, hemicellulose, and lignin as digestibility indicators was inefficient for the analyzed nutrients of both diets. Therefore, cellulose is the most suitable indicator for digestibility evaluation in Nile tilapia.

scholarly journals Ruminal parameters and ruminal degradability of feedlot sheep fed safflower grains

2018 ◽  
Vol 19 (3) ◽  
pp. 324-335
Author(s):  
Jefferson Leonardo Rocha Alves ◽  
Rafael Henrique de Tonissi e Buschinelli de Goes ◽  
Antônio Campanha Martinez ◽  
Aguinaldo Yoshio Nakamura ◽  
Jefferson Rodrigues Gandra ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Dry Matter ◽  
Ammonia Nitrogen ◽  
Neutral Detergent Fiber ◽  
Asymptotic Model ◽  
Ruminal Fermentation ◽  
Mean Values ◽  
Ruminal Ph ◽  
Significant Difference ◽  
The Times

SUMMARY This study aimed to evaluate the rumen degradability of dry matter (DM), disappearance of neutral detergent fiber (NDF) of Tifton 85 hay, and fermentation parameters (pH and ruminal ammonia nitrogen) of sheep fed diets with inclusion of safflower grains (0, 7.50 and 15%). Three mixed breed lambs with average body weight of 35.50± 1.50 kg were used. The rumen degradability of dry matter and NDF of the Tifton 85 haywas analyzedby the disappearance of DM and NDF, and the kinetic parameters adjusted according to the first order asymptotic model. The samples were incubated in the rumen in descending order (96, 48, 24, 12, 6, 3, 0 hours). The determination of ruminal pH and ruminal ammonia nitrogen (RAN) was performed before feeding and 2, 4, 6 and 8 hours after feeding. Data of ruminal fermentation were tested by analysis of variance with repeated measures in time, at 5% probability. Ruminal pH values were not statistically different. When evaluating the RAN, there was a significant difference between the times and treatments for the diet with 15% safflower. The diets presented low effective degradability of DM and NDF with mean values of 18.02% and 47.40%, respectively. The safflower grain can be used up to 7.50% in the diets without altering the degradability and ruminal fermentation.

scholarly journals PENGARUH MULTI NUTRIENT SAUCE (MNS) DENGAN DOSIS YANG BERBEDA DALAM RANSUM TERHADAP KECERNAAN PROTEIN KASAR DAN SERAT KASAR PADA DOMBA

2020 ◽  
Vol 4 (1) ◽  
pp. 14-20
Author(s):  
M Alvin Rifqi Fadilla, Erwanto, Muhtarudin, dan Agung Kusuma Wijaya
Keyword(s):  
Crude Protein ◽  
Block Design ◽  
Animal Husbandry ◽  
Protein Digestibility ◽  
Crude Fiber ◽  
Significance Level ◽  
Randomized Block Design ◽  
Significant Difference ◽  
Department Faculty ◽  
Difference Test

The purpose of this research was to determine the effect of addition of Multinutrient Sauce (MNS) in the ration on digestibility of crude protein and crude fiber in sheep.  This research was conducted in May—July 2019 at the People's Farm of Kebagusan Village, Gedong Tataan District, Pesawaran Regency.  The digestibility analysis of crude protein and crude fiber was carried out at the Laboratory of Animal Nutrition and Feed, Animal Husbandry Department, Faculty of Agriculture, University of Lampung.  This study used a randomized block design (RBD) based on sheep body weight with six groups and three treatments, namely R0 (ration without MNS); R1 (R0 + MNS 5%); and R2 (R0 + MNS 10%).  The data obtained were analyzed using ANOVA at a 5% significance level or 1% significance, and continued with further test using Least Significant Difference Test (LSD) if there is significant results.  The results showed that the addition of 10% MNS in the ration had a significant effect (P<0.05) on increasing the digestibility of crude protein and crude fiber in sheep.   Keywords: multi nutrient sauce (MNS), digestibility of crude protein, digestibility of crude fiber, sheep.

scholarly journals Performance and blood parameters of Holstein/Zebu crossbred heifers fed with two tannins sources

2020 ◽  
Vol 9 (2) ◽  
pp. e128922150
Author(s):  
Carla Giselly de Souza ◽  
Severino Gonzaga Neto ◽  
Lara Toledo Henriques ◽  
Gherman Garcia Leal Araújo ◽  
Luciana Thie Seki Dias ◽  
...  
Keyword(s):  
Milk Production ◽  
Tannic Acid ◽  
Condensed Tannin ◽  
Blood Parameters ◽  
Corn Silage ◽  
Neutral Detergent Fiber ◽  
The Other ◽  
Total Carbohydrate ◽  
Lactating Cows ◽  
Significant Difference

The present research objective was to evaluate the performance and blood parameters of lactating cows fed with a diet of two tannin sources, based on sorghum (condensed tannin)  with increasing concentrations of tannic acid (hydrolyzable tannin’s). Increasing levels of tannic acid in a sorghum-based diet for five Holstein/Zebu crossbred lactating cows were subject to a 5 × 5 Latin square experimental design. To assess the effect on cow intake, digestibility, milk production, and blood parameters. All cows received 9.87 kg/DM of corn silage as roughage and 6.38 kg of concentrate consisting of 2.58 kg/DM of ground sorghum 0.87 kg/DM of cornmeal 1.32 kg/DM of soybean bran 0.44 kg/DM of wheat bran 0.2 kg/ DM of urea and 0.18 kg/ DM of mineral mixture. Diet 1 (control) contained low-tannin sorghum and the other diets contained high-tannin sorghum. The levels of tannic acid added to the diets were established based on the quantity of condensed tannin in high-tannin sorghum. Thus, diets 2, 3, 4 and 5 were supplemented with 1.5g (13.%DM), 79.5g (2.6%DM), 157.5g (3.9%DM) and 235.5g (5.2%DM) of tannic acid, totalling 0.078, 0.156, 0.234 and 0.321 total tannin kg/day respectively, to assess the effect on microbial protein synthesis. The diets contained 35 kg of corn silage (roughage) and 6.40 kg of concentrate. The natural and dry matter intake did not significantly differ between groups, except for ether extract (EE) intake that significantly differed. The DM apparent digestibility, crude protein, EE, neutral detergent fiber, total carbohydrate, and non-fibrous carbohydrate did not differ (P > 0.05) with the increase in dietary tannin supplementation. The GOT levels increased linearly. The blood glucose, triglyceride, and cholesterol (total, LDL and HDL) levels did not significantly differ. Hemoglobin showed a significant difference. No significant difference in urea, creatinine and uric acid occurred. Diets using two tannins sources supplementation caused no decrease in the dietary intake or digestibility in the animals. The GOT level changed significantly, showing linear behavior, however below the toxicity level, without any change in the other blood parameters. Milk production decreased with the increase in dietary tannin supplementation. 

scholarly journals Deciphering Gut Microbiota Dysbiosis and Corresponding Genetic and Metabolic Dysregulation in Psoriasis Patients Using Metagenomics Sequencing

2021 ◽  
Vol 11 ◽  
Author(s):  
Shiju Xiao ◽  
Guangzhong Zhang ◽  
Chunyan Jiang ◽  
Xin Liu ◽  
Xiaoxu Wang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Target Genes ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Phosphotransferase System ◽  
Microbial Composition ◽  
Kegg Pathways ◽  
Significant Difference ◽  
Rrna Gene Sequencing ◽  
Endothelial Growth Factor

BackgroundIncreasing evidence has shown that alterations in the intestinal microbiota play an important role in the pathogenesis of psoriasis. The existing relevant studies focus on 16S rRNA gene sequencing, but in-depth research on gene functions and comprehensive identification of microbiota is lacking.ObjectivesTo comprehensively identify characteristic gut microbial compositions, genetic functions and relative metabolites of patients with psoriasis and to reveal the potential pathogenesis of psoriasis.MethodsDNA was extracted from the faecal microbiota of 30 psoriatic patients and 15 healthy subjects, and metagenomics sequencing and bioinformatic analyses were performed. The Kyoto Encyclopedia of Genes and Genomes (KEGG) database, cluster of orthologous groups (COG) annotations, and metabolic analyses were used to indicate relative target genes and pathways to reveal the pathogenesis of psoriasis.ResultsCompared with healthy individuals, the gut microbiota of psoriasis patients displayed an alteration in microbial taxa distribution, but no significant difference in microbial diversity. A distinct gut microbial composition in patients with psoriasis was observed, with an increased abundance of the phyla Firmicutes, Actinobacteria and Verrucomicrobia and genera Faecalibacterium, Bacteroides, Bifidobacterium, Megamonas and Roseburia and a decreased abundance of the phyla Bacteroidetes, Euryarchaeota and Proteobacteria and genera Prevotella, Alistipes, and Eubacterium. A total of 134 COGs were predicted with functional analysis, and 15 KEGG pathways, including lipopolysaccharide (LPS) biosynthesis, WNT signaling, apoptosis, bacterial secretion system, and phosphotransferase system, were significantly enriched in psoriasis patients. Five metabolites, hydrogen sulfide (H2S), isovalerate, isobutyrate, hyaluronan and hemicellulose, were significantly dysregulated in the psoriatic cohort. The dysbiosis of gut microbiota, enriched pathways and dysregulated metabolites are relevant to immune and inflammatory response, apoptosis, the vascular endothelial growth factor (VEGF) signaling pathway, gut-brain axis and brain-skin axis that play important roles in the pathogenesis of psoriasis.ConclusionsA clear dysbiosis was displayed in the gut microbiota profile, genetic functions and relative metabolites of psoriasis patients. This study is beneficial for further understanding the inflammatory pathogenesis of psoriasis and could be used to develop microbiome-based predictions and therapeutic approaches.

Sign in / Sign up

Export Citation Format

Share Document