scholarly journals Influence of Temperature Conditions during Growth on Bioactive Compounds and Antioxidant Potential of Wheat and Barley Grasses

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2742
Author(s):  
Mohammad-Zahirul Islam ◽  
Buem-Jun Park ◽  
Young-Tack Lee
Keyword(s):  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Growth Parameters ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Growth Conditions ◽  
Antioxidant Potential ◽  
Guaiacol Peroxidase ◽  
Dark Light ◽  
Growing Medium

Wheat and barley grasses are freshly sprouted leaves of wheat and barley seeds, and are rich sources of phytochemicals. This study was conducted to investigate the effects of day and night temperatures on the growth, bioactive compounds, and antioxidant potential of wheat and barley grasses. Briefly, each grass was cropped in an organic growing medium at 10/5 °C, 20/15 °C, and 30/25 °C (day/night temperature) in a growth chamber by maintaining specific light (12/12 h light/dark; light intensity 150 µmol photons m−2 s−1) and humidity (60%) conditions for 8 days. The highest growth parameters (height, weight, and yield) were observed at the 20/15 °C growth conditions in both types of grass. Conversely, the lowest growth parameters were observed at 10/5 °C. However, the low growth temperature of 10/5 °C resulted in increased levels of bioactive compounds (total phenol, total flavonoid, and total vitamin C), antioxidant activities (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid and 2,2-Diphenyl-1-picrylhydrazyl radical-scavenging activity)), and antioxidant enzymes (guaiacol peroxidase activity, catalase activity, and glutathione reductase) in both types of grass. Therefore, proper temperature growth conditions of wheat and barley grasses may be a convenient and efficient method to increase bioactive compounds and antioxidant potential in our diet to exploit the related health benefits.

Cationic Clitoria ternatea Seed Peptide as a Potential Novel Bioactive Molecule

2021 ◽  
Vol 28 ◽  
Author(s):  
Sreekala S. ◽  
Usha Devi Muraleedharan
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Physicochemical Characterization ◽  
Antioxidant Potential ◽  
Cationic Peptides ◽  
Cationic Peptide ◽  
Protein Database ◽  
Clitoria Ternatea

Background: While several biologics have been reported from different parts of Clitoria ternatea, a herbaceous climber of the family Fabaceae, specific production of cationic peptides other than cyclotides (<3.7 kDa) has barely been investigated or their bioactive potential looked into. Objective: To uncover potential bioactivities and characteristics of novel cationic peptides from C. ternatea seeds. Methods: C. ternatea seed cationic peptide purified by simple and cost-effective procedures was analyzed by electrophoresis and mass spectrometry. Antimicrobial efficacy was evaluated against bacterial and fungal pathogens. Antioxidant potential was quantified by in vitro antioxidant assays. Physicochemical characterization and Tandem mass spectrometry were performed. Results: An 8.5 kDa cationic peptide purified from C. ternatea seeds was active against Candida albicans, Staphylococcus aureus, Aeromonas hydrophila and Escherichia coli at a minimum inhibitory concentration in the range of 8-32 μg/ml. This activity was totally uncompromised at pH 5-8 or after 1 h of heat treatment at 70-80 ºC, but was sensitive to protease treatment. Concentration-dependent free-radical scavenging activity and ferric-reducing capacity demonstrated the antioxidant potential of the peptide. Tandem MS analysis of trypsin-digested peptide based on shotgun proteomics detected matching peptide sequences with one or two cysteine residues but had low sequence coverage (≤17%) to known sequences in the C. ternatea protein database. Taken together, the distinct characteristics of this novel 8.5 kDa peptide clearly distinguishes it from known cyclotides of C. ternatea. Conclusions: Insights have been obtained into the functional characteristics of what appears to be a novel cationic peptide from C. ternatea seeds, exhibiting significant antimicrobial and antioxidant activities.

scholarly journals PEG 6000-Stimulated Drought Stress Improves the Attributes of In Vitro Growth, Steviol Glycosides Production, and Antioxidant Activities in Stevia rebaudiana Bertoni

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1552
Author(s):  
Muhammad Arslan Ahmad ◽  
Rabia Javed ◽  
Muhammad Adeel ◽  
Muhammad Rizwan ◽  
Yuesuo Yang
Keyword(s):  
Drought Stress ◽  
Antioxidant Activities ◽  
Growth Parameters ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Stevia Rebaudiana ◽  
Total Phenolic ◽  
Steviol Glycosides ◽  
Peg 6000

This study addresses the favourable effects of drought stress imposed by polyethylene glycol (PEG) 6000 on the micropropagated shoots of Stevia rebaudiana. Various concentrations, i.e., 0, 0.5, 1, 2, and 4% of PEG 6000 were applied to the nodal shoot explants for four weeks, and the influence produced on shoots growth parameters, bioactive steviol glycosides (rebaudioside A and stevioside), and nonenzymatic antioxidant activities (total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (TAC), total reducing power (TRP) and 1,1-diphenyl-2-picrylhydrazyl(DPPH)-free radical scavenging activity (FRSA)) was elucidated. The significantly highest yield (92.4% direct shoot organogenesis) and secondary metabolites (2.94% Reb A, 2.52% ST, 95.3% DPPH-FRSA, 15.0% TPC, 13.0 µg/mg TFC, 22.3 µg/mg TAC, and 19.8 µg/mg TRP) production in response to abiotic stress elicitors was obtained in Murashige and Skoog (MS) medium treatment provided with 4% of PEG 6000. The overall trend was significant enhancement of growth dynamics and pharmaceutical compounds from control to 4% of PEG 6000 concentration as a defensive response against reactive oxygen species (ROS) produced in excess by water deficit. This is a very promising study to be employed in bioreactors to get markedly enhanced content of compounds of medicinal importance in the pharmaceutical market.

scholarly journals Isolation, characterization and evaluation of antioxidant activities of secondary metabolites producing actinomycetes of terrestrial origin

2018 ◽  
Vol 6 (3) ◽  
pp. 1017 ◽  
Author(s):  
Jessy Padma B. ◽  
Saraswathi K. ◽  
Arumugam P. ◽  
Anna Shiny R.
Keyword(s):  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Biochemical Characterization ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Streptomyces Species ◽  
Marine Origin ◽  
Terrestrial Origin

Background: Microbial secondary metabolites are important sources of natural compounds when compared to others with potential, beneficial therapeutic applications. There are chances of discovery of new Streptomyces species and new compounds from the respective genus. Due to ever and over increasing resistance of pathogenic bacteria to our current arsenal of antibiotics, a great need exists for the isolation and discovery of new antibiotics and other drug agents. Based on the above concept actinomycetes are mainly targeted for secondary metabolites production and evaluation of compounds therapeutically.Methods: Totally 15 pure isolates were obtained from two different soil samples by spread plate and quadrant streak techniques. Their colony and surface morphology were studied by morphological and biochemical characterization. Secondary metabolites were extracted by solvent extraction and the presence of bioactive compounds was detected by thin layer chromatography. The antioxidant potential was determined by Dot plot, DPPH and Phosphomolybdenum assay. The nature and the number of active compounds were identified by GC-MS analysis.Results: Among the 15 isolates, 10 isolates were found to have characteristic features of actinomycetes and 8 isolates were found to be fast growing actinomycetes. Among these 8 isolates, isolates of terrestrial origin were found to possess more bioactive compounds than those of marine origin and the four terrestrial isolates selected for evaluation of bioactive compounds and determination of antioxidant potential showed that the isolate TS 1010 had better and efficient secondary metabolite activity.Conclusions: Thus, from the above study it is seen that not only actinomycetes of marine origin, but terrestrial origin are also fast growers and possess better antioxidant and radical scavenging activity.

scholarly journals The Effect of Ascophyllum nodosum Extract on the Nutraceutical Antioxidant Potential of Vigna radiata Sprout under Salt Stress

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1216
Author(s):  
Sangeeta Kumari ◽  
Deepak Phogat ◽  
Krishnan D. Sehrawat ◽  
Ravish Choudhary ◽  
Vishnu D. Rajput ◽  
...  
Keyword(s):  
Vigna Radiata ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ascophyllum Nodosum ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Low Level ◽  
Low Concentrations

Mung bean (Vigna radiata L.) sprout is a popular fresh vegetable, tasty and high in antioxidants. To increase yield and quality after the occurrence of both abiotic and biotic stresses, the application of seaweed extracts is of great importance. Hence, this study was conducted to determine the effect of Ascophyllum nodosum extract (ANE) in the presence of salt on the antioxidant potential of V. radiata sprouts. Different concentrations of ANE viz. 0.00, 0.01, 0.05, 0.10, and 0.50% and NaCl 0, 25, 50, 75, and 100 mM alone and in combinations were tested for researching the antioxidant potential of V. radiata sprouts at 0, 24, and 36 h of sprouting. The DPPH free-radical-scavenging activity of sprouts of V. radiata was found to increase with time and peaked at 24 h of treatment. The A. nodosum extract (0.01%) could reverse the ill effect of the low level of salinity posed by up to 25 mM NaCl. The increasing salinity deteriorated the antioxidant activity using ABTS method of sprouts down to 20.45% of the control at 100 mM NaCl. The total phenolic content (TPC), total flavonoid content (TFC), and reducing power of V. radiata sprouts was found to increase till 36 h of sprouting. A slight increase in TPC, TFC and reducing power was observed when seeds were treated with low concentrations of ANE. The elevation in TPC, TFC and reducing power upon treatment with low concentrations of ANE was also noticed in sprouts in saline combinations. Alpha amylase inhibition activity was found to reach a (67.16% ± 0.9) maximum at 24 h of sprouting at a 0.01% concentration of ANE. Tyrosinase inhibition and alpha glucosidase inhibition was 88.0% ± 2.11 and 84.92% ± 1.2 at 36 h of sprouting, respectively, at 0.01% concentration of ANE. A. nodosum extract is natural, environmentally friendly, and safe, and could be used as one of the strategies to decline stress at a low level and enhance the antioxidant activities in V. radiata sprouts, thus increasing its potential to be developed as an antioxidant-based functional food.

Antioxidant Potential and Bioactive Compounds in Extract and Fractions from the Fruit of Smilax china: an Underutilised Edible Berry

2013 ◽  
Vol 709 ◽  
pp. 883-886
Author(s):  
Hai Lan Huang ◽  
Hai Yuan Wang ◽  
Shu Mei Qiu ◽  
Zong Hua Wang
Keyword(s):  
Bioactive Compounds ◽  
Soluble Fraction ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Total Flavonoids Content ◽  
Dpph Radical Scavenging

The fruit of Smilax china is an edible spherical berry. The content of some bioactive compounds, antioxidant activities in three different assays, and the relationships between these bioactive compounds and antioxidant activity of the extracts derived from the peel of the fruit of were investigated. The results showed that the ethyl acetate-soluble fraction (EAF) was the richest fraction in total phenolic, total flavonoids content. The n-butanol-soluble fraction (BF) had the highest concentration of anthocyanins. EAF and BF showed good DPPH radical-scavenging activity (DRSA) and reducing power (RP), while the crude extract (CE) possessed better hydroxyl radical-scavenging activity (HRSA) than other fractions. There were statistically significant correlations between some antioxidants and antioxidant activities.

scholarly journals Production, Characterization and Antioxidant Potential of Protease fromStreptomycessp. MAB18 Using Poultry Wastes

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Panchanathan Manivasagan ◽  
Jayachandran Venkatesan ◽  
Kannan Sivakumar ◽  
Se-Kwon Kim
Keyword(s):  
Animal Feed ◽  
Specific Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Value Added ◽  
Antioxidant Potential ◽  
Scavenging Activity ◽  
Poultry Waste

Poultry waste is an abundant renewable source for the recovery of several value-added metabolites with potential industrial applications. This study describes the production of protease on poultry waste, with the subsequent use of the same poultry waste for the extraction of antioxidants. An extracellular protease-producing strain was isolated from Cuddalore coast, India, and identified asStreptomycessp. MAB18. Its protease was purified 17.13-fold with 21.62% yield with a specific activity of 2398.36 U/mg and the molecular weight was estimated as 43 kDa. The enzyme was optimally active at pH 8–10 and temperature 50–60°C and it was most stable up to pH 12 and 6–12% of NaCl concentration. The enzyme activity was reduced when treated with Hg2+, Pb2+, and SDS and stimulated by Fe2+, Mg2+, Triton X-100, DMSO (dimethyl sulfoxide), sodium sulphite, andβ-mercaptoethanol. Furthermore, the antioxidant activities of protease were evaluated usingin vitroantioxidant assays, such as DPPH radical-scavenging activity, O2scavenging activity, NO scavenging activity, Fe2+chelating activity, and reducing power. The enzyme showed important antioxidant potential with an IC50value of78±0.28 mg/mL. Results of the present study indicate that the poultry waste-derived protease may be useful as supplementary protein and antioxidant in the animal feed formulations.

Elachi lemon (Citrus limon) peel and pulp: Antioxidant, antimicrobial, anticoagulant activities, bioactive compounds, minerals, and heavy metals

2020 ◽  
Vol 16 ◽  
Author(s):  
Shahad Saif Khandker ◽  
Alamgir Kabir ◽  
Md. Jahid Hasan ◽  
Md. Shohael Ahmed ◽  
Siew Hua Gan ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Anticoagulant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Diffusion Method ◽  
Citrus Limon ◽  
Antioxidant Power

Background: According to a number of previous studies, citrus fruits are rich in phytochemicals, minerals and bioactive compounds. Objective: The aim of this study was to investigate the antioxidant, antimicrobial and anticoagulant activities, phytonutrients, bioactive compounds, mineral and heavy metals in peel and pulp of Elachi lemon (Citrus limon). Methods: Phytochemical screening was performed to estimate the total polyphenol, flavonoid, tannin, reducing sugar, total protein and vitamin C contents. High performance liquid chromatography was performed to determine the concentrations of phenolics and ascorbic acid. Antioxidant activities were evaluated by measuring 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, reducing power assay and ferric reducing antioxidant power (FRAP) assay. Antibacterial activity was evaluated by disc diffusion method and minimum inhibitory concentration (MIC). Besides estimating heavy metals and minerals, activated partial thromboplastin time (aPTT) and prothrombin time (PT) were observed for detecting anticoagulant activity. Results: In case of antioxidant and antimicrobial activities, pulp was found to be richer. Both the peel and pulp showed similar anticoagulant activities. Among the phytonutrients, protein content was richer in peel that in pulp. Catechin, rutin, tannic, gallic and vanilic acids were detected in peel whereas in pulp quercetin, tannic and gallic acids were found. Pb, Cr, Cd were present in a negligible amount whereas, Co, Cu and Fe content were high in both the peel and pulp. Conclusion: This study exhibits that both the peel and the pulp Citrus limon is a rich source of antioxidant, antimicrobial, anticoagulant, minerals and various bioactive compounds with nutritional values.

scholarly journals Antioxidant Activities of Eleven Australian Essential Oils

2008 ◽  
Vol 3 (5) ◽  
pp. 1934578X0800300
Author(s):  
Qian Zhao ◽  
Esther Joy Bowles ◽  
Hong-Yu Zhang
Keyword(s):  
Essential Oils ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Tea Tree Oil ◽  
Tea Tree ◽  
Eucalyptus Oil ◽  
Diammonium Salt

Essential oils are gaining increasing interest due to their multiple biological activities and great potential for therapeutic use. The antioxidant effect of essential oils is of special interest in diseases with inflammatory aspects. In this paper, the antioxidant activities of eleven essential oils extracted from Australian native plants were examined by the 2,2-diphenyl-1-picryhydrazyl (DPPH) and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays. In the DPPH assay, all of the essential oils showed substantial antioxidant potential, with a radical-scavenging activity ranging from 12.9% ± 0.3% to 86.9% ± 0.2% at the concentration of 1.6 × 10−2 mL/mL. In the ABTS assay, lemon-scented tea-tree oil ( Leptospermum petersonii Bailey) (80.6% ± 0.7%), Australian blue cypress oil ( Callitris intratropica R. T. Baker & H. G. Smith) (78.6% ± 1.3%), lemon-scented eucalyptus oil ( Eucalyptus citriodora Hook.) (56.7% ± 0.9%) and lemon-scented ironbark oil ( Eucalyptus staigeriana F. Muell. ex Bailey) (58.9% ± 0.8%) exhibited relatively high radical-scavenging activities at the concentration of 1.6 × 10−2 mL/mL. Taken together, in both DPPH and ABTS assays, lemon-scented tea-tree oil (with IC50 of 1.5 × 10−3 mL/mL and 1.5 × 10−3 mL/mL, respectively), Australian blue cypress oil (with IC50 of 9.5 × 10−3 mL/mL and 3.0 × 10−3 mL/mL, respectively), lemon-scented eucalyptus oil (with IC50 of 4.8 × 10−3 mL/mL and 8.9 × 10−3 mL/mL, respectively) and lemon-scented ironbark oil (with IC50 of 6.4 × 10−3 mL/mL and 7.0 × 10−3 mL/mL, respectively) showed the highest antioxidant potential of the essential oils tested. By comparison, the antioxidant capacity of vitamin E had IC50 values of 5.3 × 10−5 mL/mL and 4.3 × 10−6 mL/mL in the DPPH and ABTS tests respectively.

scholarly journals Evolution of antioxidant properties of orange beverage during storage: Effects of time and temperature

2021 ◽  
Vol 25 (2) ◽  
pp. 293-300
Author(s):  
Ouahiba Soufi ◽  
Mostapha Bachir-Bey ◽  
Saida Kessi ◽  
Djamila Gani ◽  
Nourredine Touati
Keyword(s):  
Bioactive Compounds ◽  
Storage Temperature ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Duration Of Storage ◽  
Antioxidant Parameters ◽  
Storage Effects

Abstract Fruit beverages are the most consumed refreshing products worldwide. The quality of these drinks is not only influenced by the initial composition of the product but also by conditions and duration of storage. The purpose of the present investigation was to evaluate the incidence of two conservation parameters (temperature and time) on some bioactive compounds (phenolic, flavonoid, and carotenoid contents) and antioxidant capacity, measured by free radical scavenging activity (RSA) and ferric reducing power (FRP), on orange beverage during storage. The industrial drinking product freshly procured were subjected to storage under variable temperatures (5, 25, 35, and 45 °C) then analyses of bioactive compounds contents and antioxidant activity were performed periodically (0, 10, 20, and 30 days). The results showed that the initial orange beverage was endowed with interesting phenolic compound contents and considerable antioxidant potential. In the exception of 5 °C, which provided steady antioxidant parameters, all other temperatures induced decreases of analyzed phytochemicals and antioxidant activities proportionally with raising of storage temperature. The duration of storage for the temperatures 25, 35, and 45 °C also affected negatively antioxidant parameters in a proportional manner to time. The orange beverage was an interesting natural source of bioactive compounds but the duration of storage in relation to temperature must be chosen properly.

Antioxidant capacity of hydroalcoholic extracts of Grewia serrulata DC and Grewia nervosa (Lour.) Panigrahi

2018 ◽  
Vol 9 (1) ◽  
pp. 121
Author(s):  
Mentham Ramesh ◽  
Chandu Babu Rao
Keyword(s):  
Total Phenolic Content ◽  
Superoxide Radical ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content

The current investigation is intended to evaluate the content of phytochemical constituents and antioxidant potential of hydroalcoholic extracts of stem and root of Grewia serrulata DC (HAESGS & HAERGS) and leaf and bark of Grewia Nervosa (Lour.) panigrahi (HAELGN & HAEBGN). Initially, all the extracts at different concentrations were estimated for their total phenolic content and total flavonoid content. The study was further extended for their antioxidant potential evaluation using various in vitro methods such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical scavenging assays. The total phenolic content (mg gallic acid equivalent per gram of extract) was high in HAELGN (170.82±0.19) and HAERGS (123.00±0.48) than HAESGS (111.2±0.26) and HAEBGN (119.60±0.23). The total flavonoid content (mg quercetin equivalent per gram) is greater in HAERGS (71.24±0.50) and HAESGS (65.68±0.27) than HAELGN (55.82±0.35) and HAEBGN (62.38±0.45). The IC50 values (µg/ml) of different plant extracts inferred that DPPH radical scavenging activity is greater in HAELGN (42.91±0.88) and HAEBGN (53.87±0.35) than HAESGS (126.73±1.20) and HAERGS (88.87±1.25). However, hydroxyl and superoxide radical scavenging activity is more in HAERGS (135.41±1.19 & 88.00±1.42) and HAELGN (172.28±1.91 & 108.163±1.09) than HAESGS (237.3±1.65 & 110.074±0.87) and HAEBGN (204.7±1.04 & 125.54±1.07). The results of present comprehensive analysis demonstrated that both the plants Grewia serrulata DC and Grewia Nervosa (Lour.) panigrahi possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a valid source of natural antioxidants and might be utilized for pharmacological screening of various therapeutic activities. Keywords: Grewia serrulata; Grewia Nervosa; Total Phenolic content; Total flavonoid content; Antioxidant potential

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