Chemometric Profiling and Bioactivity of Verbena (Aloysia citrodora) Methanolic Extract from Four Localities in Tunisia

This research aimed to study the chemical composition of Aloysia citrodora methanolic extract and its biological activities as an antioxidant, and its antibacterial, antifungal and anti-inflammatory activities based on four bioclimatic collection stages. The contents of total phenols, total flavonoids and total tannins were determined. Nine phenolic compounds were identified by LC-DAD–ESI-MS/MS. The major compound was acteoside, a phenylpropanoid which represented about 80% of the methanolic fraction in the various regions. The antioxidant activities of different locations were measured by different analytical assays, such as DPPH, ABTS and iron reducing power. The results showed that phenolic compounds and antioxidant activities varied with climatic and environmental factors. Moreover, there was a significant dependency between regions and biological activities. The use of a principal component analysis showed that there was a close relationship among phenylpropanoids, phenolic compounds and the studied biological activities.

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Antioxidant and antityrosinase activities of milky white mushroom

Bangladesh Journal of Botany ◽

10.3329/bjb.v48i4.49054 ◽

2019 ◽

Vol 48 (4) ◽

pp. 1065-1073

Author(s):

Nuhu Alam ◽

Md Maniruzzaman Sikder ◽

Md Abdul Karim ◽

Sheikh Md Ruhul Amin

Keyword(s):

Gallic Acid ◽

Methanolic Extract ◽

Antioxidant Activities ◽

Biological Activities ◽

Reducing Power ◽

Positive Control ◽

Hot Water ◽

Biochanin A ◽

Calocybe Indica ◽

Β Carotene

It was intended to investigate the biological activities of different solvent extracts of the fruiting bodies of Calocybe indica (P & C). The bioactive compounds were extracted using acetone, methanol, and hot water. The antioxidant activities of three different extracts at 0.5-20.0 mg/ml of C. indica on β-carotene-linoleic acid ranged from 65.83 - 92.56, 62.79 - 93.06 and 61.42 - 92.12%, respectively. The highest (2.825) reducing power inhibition was recorded in methanolic extract, while the lowest (2.332) was in hot water at the concentration of 8 mg/ml. The free radicle scavenging activity was more pronounced in methanolic extract. Chelating effect of C. indica was significantly strong as compared to positive control. The xanthine oxidase and tyrosinase inhibitory activity of the acetone, methanol, and hot water extracts of C. indica increased with increasing concentration. Thirteen phenolic compounds i.e. biochanin-A, caffeic acid, chlorogenic acid, ferulic acid, formononetin, gallic acid, hesperetin, homogentisic acid, naringenin, naringin, protocatechuic acid, resveratrol and vanillin were detected from acetonitrile-hydrochloric acid extract. The result indicated that the maximum and minimum concentrations were recorded for gallic acid (29 μg/g) and formononetin (10 μg/g). Thus, it could be suggested that C. indica has the potential to be used as antioxidant and tyrosinase protection system of the human body against oxidative damage and other complications.

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Comparison of Phenolic Compounds and the Antioxidant Activities of Fifteen Chrysanthemum morifolium Ramat cv. ‘Hangbaiju’ in China

Antioxidants ◽

10.3390/antiox8080325 ◽

2019 ◽

Vol 8 (8) ◽

pp. 325 ◽

Cited By ~ 5

Author(s):

Jinyan Gong ◽

Bingquan Chu ◽

Lingxiao Gong ◽

Zhongxiang Fang ◽

Xiaoxu Zhang ◽

...

Keyword(s):

Principal Component Analysis ◽

High Performance Liquid Chromatography ◽

Phenolic Compounds ◽

High Performance ◽

Antioxidant Activities ◽

Principal Component ◽

Chrysanthemum Morifolium ◽

Caffeoylquinic Acids ◽

Caffeoylquinic Acid ◽

Chrysanthemum Morifolium Ramat

This study investigated the phenolic compounds of 15 Chrysanthemum morifolium Ramat cv. ‘Hangbaiju’, including 6 ‘Duoju’ and 9 ‘Taiju’, using high performance liquid chromatography (HPLC). The antioxidant activities of these ‘Hangbaiju’ were estimated by DPPH, ABTS and FRAP assays. Results show that a total of 14 phenolic compounds were detected in these flowers, including 3 mono-caffeoylquinic acids, 3 di-caffeoylquinic acids, 1 phenolic acid and 7 flavonoids. ‘Duoju’ and ‘Taiju’ possess different concentrations of phenolic compounds, and ‘Taiju’ exhibits higher caffeoylquinic acids and stronger antioxidant activities than ‘Duoju’. Caffeoylquinic acids show a strong correlation with the antioxidant activities of the samples. Principal component analysis (PCA) reveals an obvious separation between ‘Duoju’ and ‘Taiju’, using phenolic compounds as variables. Apigenin-7-O-glucoside, 3,5-di-O-caffeoylquinic acid, luteolin and acacetin were found to be the key phenolic compounds to differentiate ‘Duoju’ from ‘Taiju’.

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Comparative analysis of antioxidant activity and phenolic compounds in the fruits of Aronia spp.

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1360 ◽

2020 ◽

Vol 14 ◽

pp. 393-401

Author(s):

Yulia Vinogradova ◽

Olena Vergun ◽

Olga Grygorieva ◽

Eva Ivanišová ◽

Ján Brindza

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Phenolic Acids ◽

Antioxidant Activities ◽

Biological Activities ◽

Reducing Power ◽

Biologically Active ◽

Polyphenol Compounds ◽

Dpph Method ◽

Ethanol Extracts

Chokeberry (Aronia Medik.) is a non-traditional fruit plant known as a rich source of biologically active compounds and inhibits the numerous biological activities. We compared the antioxidant activity and phenolic compounds of fruits between widely cultivated Aronia mitschurinii (AM-TCH, from Tchekhov district; AM-D, from Dmitrov district; AM-OZ, from Orekhovo-Zuevsky district of Moscow region, Russia) and introduced North American Aronia species (Aronia arbutifolia (AA-M), A. melanocarpa (AML-M), A. × prunifolia (AP-M), which have not been planted yet in the arboretum of Main Botanical Garden of the Russian Academy of Sciences (Moscow). Studying samples were collected in their secondary distribution range. Ethanolic extracts were determined for antioxidant capacity (antioxidant activity by DPPH and phosphomolybdenum methods, the total content of polyphenols, flavonoids, phenolic acids) and measured spectrophotometrically. As standards were used Trolox (TE) for antioxidant activities, gallic acid (GAE) for polyphenol content, quercetin (QE) for flavonoid content, caffeic acid (CAE) for phenolic acid content. The antioxidant activity by DPPH method in ethanol extracts of investigated plants was from 6.96 (AM-D) to 8.89 (AM-OZ) mg TE.g-1 DW. Reducing the power of investigated extracts exhibited activity from 151.47 (AM-OZ) to 297.8 (AA-M) mg TE.g-1 DW. The content of polyphenol compounds determined from 25.98 (AM-TCH) to 54.39 (AA-M) mg GAE.g-1 DW, phenolic acids content was from 7.76 (AP-M) to 11.87 (AM-D) mg CAE.g-1 DW and the content of flavonoids detected from 8.12 (AM-OZ) to 16.62 (AM-D) mg QE.-1 DW. Obtained data showed a strong correlation between the content of polyphenol compounds and reducing the power of extracts (r = 0.700), between flavonoids and phenolic acids (r = 0.771) and also between phenolic acids and reducing power (r = 0.753) in Aronia ethanol extracts. Fruits of investigated species of Aronia can be propagated as a source of polyphenol compounds with antioxidant activity and obtained results may use for farther pharmacological study.

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Chemical Analysis, Phenolic Content, and Antioxidant Activities of Aqueous and Organic Moroccan Juglans regia L. Bark Extracts

Current Bioactive Compounds ◽

10.2174/1573407216666200203103354 ◽

2020 ◽

Vol 16 (9) ◽

pp. 1328-1339

Author(s):

Mohamed Boulfia ◽

Fatima Lamchouri ◽

Hamid Toufik

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Antioxidant Activities ◽

Juglans Regia ◽

Reducing Power ◽

Principal Component ◽

Antioxidant Compounds ◽

Phytochemical Analysis ◽

Strongly Correlated

Background: The present study was conducted on the Moroccan Juglans regia L. bark, in comparison with other Juglans regia collected in other countries, to study the effects of the use of different extraction solvents (water, ethanol, acetone, and diethyl ether), aqueous and organic extraction method, hot and cold extraction by maceration modalities on the presence and content of the different phytochemicals, and on the antioxidant activity. Methods: Various solvents of increasing polarity were used for the preparation of aqueous and organic extracts. Afterwards, a phytochemical screening, an estimation of phenolic compounds, flavonoids, and tannins contents was conducted. The in vitro antioxidant activity was carried out by five tests: H2O2, ABTS, FRAP, DPPH, and reducing power. Subsequently, Principal Component Analysis (PCA) was used to establish the correlation between phenol content and antioxidant activity. Results: Phytochemical analysis showed that the 9 extracts prepared are characterized by the presence of flavonoids, catechic tannins, saponins, anthraquinones, and quinones, and that the macerated acetone extract contains the highest content of phenolic compounds (327.972±0.06 μgEAG/mgE), flavonoids (1267.981±2.911 μgEQ/mgE), and tannins (38.056±1.886 μgEC/gE). The best antioxidant activity was obtained with the macerated acetone which was found to be the most active via the five different tests, with a percentage of scavenging of H2O2 equal at 24.13±1.81, a higher value of ABTS equal to 602.29±0.34 μgET/mgE. PCA allowed us to deduce that phenolic compounds, flavonoids, and tannins are strongly correlated with antioxidant activity. Conclusion: Moroccan Juglans regia may be one of the potential sources of antioxidant compounds.

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Comparison of Phenolic Compounds and Antioxidant Activities of Fifteen Chrysanthemum morifolium Ramat cv. ‘Hangbaiju’ in China

10.20944/preprints201906.0308.v1 ◽

2019 ◽

Author(s):

Jinyan Gong ◽

Bingquan Chu ◽

Lingxiao Gong ◽

Zhongxiang Fang ◽

Xiaoxu Zhang ◽

...

Keyword(s):

Principal Component Analysis ◽

High Performance Liquid Chromatography ◽

Phenolic Compounds ◽

High Performance ◽

Antioxidant Activities ◽

Principal Component ◽

Chrysanthemum Morifolium ◽

Caffeoylquinic Acids ◽

Caffeoylquinic Acid ◽

Chrysanthemum Morifolium Ramat

This study investigated the phenolic compounds of 15 Chrysanthemum morifolium Ramat cv. ‘Hangbaiju’, including 6 ‘Duoju’ and 9 ‘Taiju’ using high performance liquid chromatography. The antioxidant activities of these ‘Hangbaiju’ were estimated by DPPH, ABTS and FPAR assays. Results showed that a total of 14 phenolic compounds were detected in these flowers, including 3 mono-caffeoylquinic acids, 3 di-caffeoylquinic acids, 1 phenolic acid and 7 flavonoids. ‘Duoju’ and ‘Taiju’ possessed different concentration of phenolic compounds, and ‘Taiju’ exhibited higher caffeoylquinic acids and stronger antioxidant activities than ‘Duoju’. Caffeoylquinic acids showed a strong correlation with the antioxidant activities of the samples. Principal component analysis revealed an obvious separation between ‘Duoju’ and ‘Taiju’ using phenolic compounds as variables. Apigenin-7-O-glucoside, 3,5-di-O-caffeoylquinic acid, luteolin and acacetin were found to be the key phenolic compounds to differentiate ‘Duoju’ from ‘Taiju’.

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Chemical profiles and biological properties of methanol extracts of Allium pallens L. from different localities in Turkey

Archives of Biological Sciences ◽

10.2298/abs200226013e ◽

2020 ◽

Vol 72 (2) ◽

pp. 193-201

Author(s):

Ahmet Emir ◽

Ceren Emir

Keyword(s):

Phenolic Compounds ◽

Antioxidant Activities ◽

Biological Activities ◽

Chemical Constituents ◽

Principal Component ◽

Biological Properties ◽

Total Phenolic ◽

Underlying Structure ◽

Allium Species ◽

Phenolic Contents

Many species of the Allium genus, principally the cultivated forms, are widely used as vegetables, spices and natural therapeutics due to their beneficial health properties. This study aimed to identify the phenolic composition and biological activities of the bulb, stem and flower parts of Allium pallens L., collected from two different localities. A total of 28 phenolic compounds were investigated by LC-ESI-MS/MS, and gallic acid, 4-hydroxybenzoic acid, and benzoic acid were found to be the major phenolic compounds in the plants from both locations. Total phenolic- and flavonoid-content analyses of samples were carried out using spectrophotometry, and the stem extracts were found to be rich in phenolics. DPPH, ABTS, FRAP and CUPRAC assays were used to determine the antioxidant capacities of the extracts. A linear relation was observed between the phenolic contents of the extracts and their antioxidant activities, and the stem extracts of plants from both locations were found to have potent antioxidant capacity. The inhibitory activities of the extracts against acetylcholinesterase, butyrylcholinesterase and tyrosinase were determined using a 96-well microplate reader. The antibutyrylcholinesterase activity of the extracts was found to be the highest. The outcomes of these investigations were further explored, and the underlying structure of multivariate data was revealed using principal component analysis. This study presents the distribution of chemical constituents and biological activities of the different parts of A. pallens, and also contributes to further investigations of Allium species.

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Influence of processing on the phenolic compounds of feijoa pulp (Feijoa sellowiana)

Journal of Food Bioactives ◽

10.31665/jfb.2020.11238 ◽

2020 ◽

Vol 11 ◽

Author(s):

Ana Carolina Leme Castelucci ◽

Nataly Maria Viva De Toledo ◽

Fernanda Francetto Juliano ◽

Paula Porrelli Moreira Da Silva ◽

Marta Helena Fillet Spoto

Keyword(s):

Principal Component Analysis ◽

Antioxidant Activity ◽

Phenolic Compounds ◽

Total Phenolic Content ◽

Phenolic Content ◽

Principal Component ◽

Total Phenolic ◽

Total Flavonoids ◽

Phenolic Composition ◽

And Control

The effect of processing on potential changes of antioxidant activity (DPPH and ABTS), total phenolic content (TPC) and total flavonoids (FLAV) of feijoa pulp during storage was investigated. In addition, possible modifications in the phenolic composition of the pulp. The pulp was subjected to refrigeration (control, 5 °C), pasteurization (85 °C/5 min) and irradiation (2.0 kGy). TPC, FLAV, DPPH and ABTS were measured during 28 days, whereas the identification and quantification of phenolic compounds were conducted after processing. The results were submitted to principal component analysis (SAS 9.4). Pasteurization preserved TPC, DPPH and ABTS for 21 days, while in the irradiated samples and control, the values were decreased and FLAV were maintained at high levels. The profile of phenolic compounds was different for each sample with six compounds being identified.

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Chemical Constituents Isolated from Rhododendron ungernii with Antioxidant Profile

The Natural Products Journal ◽

10.2174/2210315508666181024114812 ◽

2019 ◽

Vol 9 (3) ◽

pp. 238-243 ◽

Cited By ~ 2

Author(s):

Emine Dede ◽

Nusret Genc ◽

Mahfuz Elmastas ◽

Huseyin Aksit ◽

Ramazan Erenler

Keyword(s):

Antioxidant Activities ◽

Biological Activities ◽

Chemical Constituents ◽

Reducing Power ◽

Scavenging Activity ◽

Isolation And Identification ◽

Reduced Pressure ◽

Scavenging Effect ◽

Pharmaceutical Industries ◽

Dpph Scavenging

Background: Plant in Rhododendron genus that contains phenolic compounds has been used in traditional medicine and revealed considerable biological activities. Objective: Isolation and identification of antioxidant natural products from Rhododendron ungernii. Methods: Rhododendron ungernii Trautv. flowers were collected and dried in shade. The dried flowers were extracted with methanol for 3 days. The solvent was removed by reduced pressure to yield the extract which was subjected to column chromatography (Sephadex LH-20, C18 reversed phase column) to isolate catechin-7-O-glucoside (1), quercetin-3-O-β-galactoside (2), quercetin-3-O- β-xyloside (3), farrerol (4), myricetin (5), and quercetin (6). The structures of isolated compounds were elucidated by spectroscopic methods such as 1D-NMR, 2D-NMR, and LC-TOF/MS. DPPH scavenging effect, ABTS+ scavenging activity, and reducing power (FRAP) were performed for antioxidant assays of isolated natural compounds. Results: Isolated flavonoids displayed the outstanding antioxidant activities. Catechin-7-O-glucoside (1) and quercetin-3-O-β-galactoside (2) (IC50, 3.66 µg/mL) had the most DPPH• scavenging effect among the compounds. The highest ABTS•+ scavenging activity (IC50, 1.41 µg/mL) and reducing power effect (6.05 mmol TE/g comp) were observed for myricetin (5). Conclusion: R. ungernii extract and isolated compounds could be a promising antioxidant for food and pharmaceutical industries.

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Relationships Between the Content of Phenolic Compounds and the Antioxidant Activity of Polish Honey Varieties as a Tool for Botanical Discrimination

Molecules ◽

10.3390/molecules26061810 ◽

2021 ◽

Vol 26 (6) ◽

pp. 1810

Author(s):

Monika Kędzierska-Matysek ◽

Małgorzata Stryjecka ◽

Anna Teter ◽

Piotr Skałecki ◽

Piotr Domaradzki ◽

...

Keyword(s):

Principal Component Analysis ◽

Antioxidant Activity ◽

Phenolic Compounds ◽

Caffeic Acid ◽

Principal Component ◽

Total Content ◽

Component Analysis ◽

Total Phenolic ◽

Hydroxybenzoic Acid ◽

Coumaric Acid

The study compared the content of eight phenolic acids and four flavonoids and the antioxidant activity of six Polish varietal honeys. An attempt was also made to determine the correlations between the antioxidant parameters of the honeys and their polyphenol profile using principal component analysis. Total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (ABTS) and reduction capacity (FRAP) were determined spectrophotometrically, and the phenolic compounds were determined using high-performance liquid chromatography (HPLC). The buckwheat honeys showed the strongest antioxidant activity, most likely because they had the highest concentrations of total phenols, total flavonoids, p-hydroxybenzoic acid, caffeic acid, p-coumaric acid, vanillic acid and chrysin. The principal component analysis (PCA) of the data showed significant relationships between the botanic origin of the honey, the total content of phenolic compounds and flavonoids and the antioxidant activity of the six Polish varietal honeys. The strongest, significant correlations were shown for parameters of antioxidant activity and TPC, TFC, p-hydroxybenzoic acid, caffeic acid and p-coumaric acid. Analysis of four principal components (explaining 86.9% of the total variance), as a classification tool, confirmed the distinctiveness of the Polish honeys in terms of their antioxidant activity and content of phenolic compounds.

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Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthiumL.) Essential Oils and Phenolics

Journal of Chemistry ◽

10.1155/2015/804658 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 35

Author(s):

Kamel Msaada ◽

Nidhal Salem ◽

Olfa Bachrouch ◽

Slim Bousselmi ◽

Sonia Tammar ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oil ◽

Essential Oils ◽

Methanolic Extract ◽

Biological Activities ◽

Reducing Power ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Oil Composition ◽

Antioxidant And Antimicrobial Activities

The aim of this study was to determine the chemical variability of wormwood extracts as affected by the growing region. Antioxidant and antimicrobial activities were also investigated. The essential oil composition variability ofA. absinthiumL. aerial parts collected from four different Tunisian regions was assessed by gas chromatography (GC/FID) and by gas chromatography mass spectrometry (GC/MS). In addition, total polyphenols, flavonoids, and condensed tannins as well as antioxidant, antibacterial, and antifungal activities of methanolic extract and essential oils were undertaken. Chromatographic analysis of wormwood essential oils showed the predominance of monoterpene hydrocarbons represented mainly by chamazulene. RP-HPLC analysis of wormwood methanolic extract revealed the predominance of phenolic acids. Antiradical activity was region-dependant and the methanolic extract of Bou Salem region has the strongest activity (CI50=9.38±0.82 µg/mL). Concerning the reducing power, the methanolic extract of Bou Salem, Jérissa, and Boukornine regions was more active than the positive control. Obtained results of antimicrobial activities showed that wormwood essential oil is endowed with important antibacterial activity which was strongly related to the organoleptic quality of oil which appeared strongly region-dependant.A. absinthiumL. EOs investigated are quite interesting from a pharmaceutical standpoint because of their biological activities.

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