Botanical Origin, Pollen Profile, and Physicochemical Properties of Algerian Honey from Different Bioclimatic Areas

The palynological and physicochemical analysis of 62 honey samples produced in different biogeographical areas of Algeria was conducted. Results showed high variety in the botanical origin of samples and their physicochemical profile. Twenty-six samples were polyfloral honey, 30 were unifloral honey from different botanical sources such as Eucalyptus, Citrus, Apiaceae, Punica, Erica, Rosmarinus, Eriobotrya, or Hedysarum, and 6 were characterized as honeydew honey. Pollen analysis allowed the identification of 104 pollen types belonging to 51 botanical families, whereas the physicochemical profile showed important variations between samples. Multivariate techniques were used to compare the characteristics of samples from different biogeographical areas, showing significant differences between humid-area samples, located in the northeast of the country, and samples taken in semiarid, subhumid, and arid zones. Principal-component analysis (PCA) extracted nine components explaining 72% of data variance, being 30%, the sum of Component 1 and Component 2. The plot of both components showed samples grouped upon botanical and geographical origin. The results of this paper highlighted the great variability in honey production of Algeria, evidencing the importance of honey characterization to guarantee authenticity and to valorize local production.

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Melissopalynological analysis of honey produced by two species of stingless bees in Lombok Island, Indonesia

Nusantara Bioscience ◽

10.13057/nusbiosci/n120203 ◽

2020 ◽

Vol 12 (2) ◽

Author(s):

Lalu Zulkan Jayadi ◽

Ratna Susandarini

Keyword(s):

Stingless Bees ◽

Geographical Origin ◽

Botanical Origin ◽

Pollen Type ◽

Pollen Content ◽

Pollen Types ◽

Unifloral Honey ◽

Melissopalynological Analysis ◽

Lombok Island ◽

Number Of Plant Species

Abstract. Jayadi LZ, Susandarini R. 2020. Melissopalynological analysis of honey produced by two species of stingless bees in Lombok Island, Indonesia. Nusantara Bioscience 12: 97-108. Honey is a natural product with a variety of benefits that commonly used as food sweeteners, health supplements, and traditional medicine. There has been no comprehensive publication regarding the diversity of pollen contained in honey produced by stingless bee Tetragonula laeviceps and Heterotrogona itama from Lombok. This study aimed to reveal the diversity of pollen content in honey through melissopalynological analysis of honey samples produced by two species of stingless bees T. laeviceps and H. itama in Lombok Island. This melissopalynological study was performed on honey samples obtained from nine villages from nine sub-districts representing the three districts on Lombok Island. Pollen extraction from honey was carried out using standard methods for melissopalynological analysis. The diversity of pollen recovered from honey varied from 15 to 41 pollen types. Total number of plant species whose pollen was recovered from honey samples was 127 which consisted of 61 families. The occurrence of predominant pollen type in particular unifloral honey samples indicated their botanical origin and presumed geographical origin of honey. Results of this study are useful in confirming botanical origin of honey and generating information on plants potential as food source for sustainable beekeeping in Lombok Island.

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Melissopalynological and physicochemical analysis of honey samples from Prayagraj District, Uttar Pradesh

10.35535/acpa-2020-0004 ◽

2020 ◽

pp. 123-133

Author(s):

Vibhasa Shukla ◽

Ajay Kumar

Keyword(s):

Physicochemical Parameters ◽

Brassica Campestris ◽

Limit Of Detection ◽

Uttar Pradesh ◽

Physicochemical Analysis ◽

Rural And Urban ◽

Pollen Types ◽

Multifloral Honey ◽

Unifloral Honey ◽

Cadmium Lead

In melissopalynological and physicochemical analyses of 14 honey samples collected from different rural and urban localities of Prayagraj District, Uttar Pradesh, 43 pollen types were identified and categorized as predominant (above 45%), secondary (16–45%), important minor (3–15%) and minor (below 3%). Five of the 14 samples were classified as unifloral due to the presence of predominant pollen types (above 45%), and the other 9 as multifloral. Brassica campestris and Coriandrum sativum were the predominant pollen types in unifloral honey; 14 pollen types were documented in the secondary pollen types (16–45%) in multifloral honey. The analyzed physicochemical parameters included pH (mean 3.40 ± 0.15 to 4.74 ± 0.4), electrical conductivity (mean 0.13 ± 0.03 to 1.39 ± 0.17 mS/cm), total dissolved solids (120 ± 1.23 to 1260 ± 1 ppm), moisture content (12.17 ± 1.39 to 24.78 ± 1.54 mg/100 g) and ash content (0.15 ± .04 to 1.68 ± .27 mg/100g). The color of the honey ranged from water-white to dark amber. Among the minerals, magnesium was found to be most abundant (9 ± 0 to 11.8 ± 0 mg/kg), followed by iron (2.0 ± 0 to 4.8 ± 0 mg/kg) and zinc (0.39 ± 0 to 0.63 ± 0 mg/kg). The heavy metals cadmium, lead, copper and arsenic were below the limit of detection (< 0.01 mg/kg) in all honey samples except in samples H6 (Cd 0.01 mg/kg, Pd 0.27 mg/kg), H11 (Cd 0.02 mg/kg, Pd 0.05 mg/kg), H13 (Pd 0.02 mg/kg) and H14 (Cd 0.01 mg/kg, Pd 0.04 mg/kg). The physicochemical parameters varied significantly (p < 0.05) between samples. Findings of the present study indicate that the honey is of good quality and should be used in projects for commercialization of regional honey.

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Sensorial, Melissopalynological and Physico-Chemical Characteristics of Honey from Babors Kabylia’s Region (Algeria)

Foods ◽

10.3390/foods10020225 ◽

2021 ◽

Vol 10 (2) ◽

pp. 225

Author(s):

Asma Ghorab ◽

María Shantal Rodríguez-Flores ◽

Rifka Nakib ◽

Olga Escuredo ◽

Latifa Haderbache ◽

...

Keyword(s):

Sensory Properties ◽

Chemical Components ◽

Chemical Characteristics ◽

Pollen Spectrum ◽

Botanical Origin ◽

Flavonoid Content ◽

Chemical Parameters ◽

High Tendency ◽

Physico Chemical ◽

Pollen Types

This study aimed to characterize the honeys of Babors Kabylia through sensory, melissopalynological and physico-chemical parameters. Thirty samples of honey produced in this region were collected over a period of two years and analyzed. All the samples presented physico-chemical parameters in conformity with legislation on honey quality, with few exceptions, linked mainly to beekeeping management. The pollen spectrum revealed a great diversity with 96 pollen types. The main pollen types were spontaneous species as Fabaceae (Hedysarum, Trifolium, Genisteae plants), Asteraceae plants, Ericaceae (Erica arborea L.) or Myrtus and Pistacia. The sensory properties of samples showed a high tendency to crystallization, the colors were from white to brown, but most of them had gold color. Smell and odor corresponded mainly to vegetal and fruity families and in taste perceptions besides sweetness highlighted sourness and saltiness notes. Seventeen samples were polyfloral, one was from honeydew and twelve were monofloral from heather, genista plants, sulla, blackberry or Asteraceae. Heather and the honeydew samples showed the darkest color, the highest electrical conductivity and phenol and flavonoid content. A statistical analysis based on the most representative pollen types, sensory properties and some physico-chemical components allowed the differentiation of honey samples in terms of botanical origin.

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Relationships Linking the Colour and Elemental Concentrations of Blossom Honeys with Their Antioxidant Activity: A Chemometric Approach

Agriculture ◽

10.3390/agriculture11080702 ◽

2021 ◽

Vol 11 (8) ◽

pp. 702

Author(s):

Monika Kędzierska-Matysek ◽

Anna Teter ◽

Małgorzata Stryjecka ◽

Piotr Skałecki ◽

Piotr Domaradzki ◽

...

Keyword(s):

Antioxidant Activity ◽

Physicochemical Properties ◽

Black Locust ◽

Antioxidant Properties ◽

Principal Component ◽

Botanical Origin ◽

Antioxidant Parameters ◽

Colour Parameters ◽

Hue Angle ◽

Chemometric Approach

The antioxidant activity of honey depends on the botanical origin, which also determines their physicochemical properties. In this study, a multivariate analysis was used to confirm potential relationships between the antioxidant properties and colour parameters, as well as the content of seven elements in five types of artisanal honey (rapeseed, buckwheat, linden, black locust, and multifloral). The type of honey was found to significantly influence most of its physicochemical properties, colour parameters, and the content of potassium, manganese and copper. Antioxidant parameters were shown to be significantly positively correlated with redness and concentrations of copper and manganese, but negatively correlated with the hue angle and lightness. The principal component analysis confirmed that the darkest buckwheat honey had the highest antioxidant activity in combination with its specific colour parameters and content of antioxidant minerals (manganese, copper and zinc). The level of these parameters can be potentially used for the identification of buckwheat honey.

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The Use of Right Angle Fluorescence Spectroscopy to Distinguish the Botanical Origin of Greek Common Honey Varieties

Applied Sciences ◽

10.3390/app11094047 ◽

2021 ◽

Vol 11 (9) ◽

pp. 4047

Author(s):

Marinos Xagoraris ◽

Panagiota-Kyriaki Revelou ◽

Eleftherios Alissandrakis ◽

Petros A. Tarantilis ◽

Christos S. Pappas

Keyword(s):

Fluorescence Spectroscopy ◽

External Validation ◽

Three Dimensional ◽

Excitation Wavelength ◽

Botanical Origin ◽

Linear Discriminant ◽

Classification Score ◽

Hydroxybenzoic Acids ◽

Unifloral Honey ◽

Novel Method

The standardization of the botanical origin of honey reflects the commercial value and quality of honey. Nowadays, most consumers are looking for a unifloral honey. The aim of the present study was to develop a novel method for honey classification using chemometric models based on phenolic compounds analyzed with right angle fluorescence spectroscopy, coupled with stepwise linear discriminant analysis (LDA). The deconstructed spectrum from three-dimensional-emission excitation matrix (3D-EEM) spectra provided a correct classification score of 94.9% calibration and cross-validation at an excitation wavelength (λex) of 330 nm. Subsequently, a score of 81.4% and 79.7%, respectively, at an excitation wavelength (λex) of 360 nm was achieved. Each chemometric model confirmed its power through the external validation with a score of 82.1% for both. Differentiation could be correlated with hydroxycinnamic and hydroxybenzoic acids, which absorb in this region of the spectrum. Fluorescence spectroscopy constitutes a rapid and sensitive technique, which, when combined with the stepwise algorithm and LDA method, can be used as a reliable and predictive authentication tool for honey. This study indicates that the developed methodology is a promising technique for determination of the botanical origin of common Greek honey varieties. Our long-term ambition is to support producers and suppliers to remain in a competitive national and international market.

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Authentication of the botanical origin of unifloral honey by infrared spectroscopy coupled with support vector machine algorithm

Physica Scripta ◽

10.1088/0031-8949/2014/t162/014042 ◽

2014 ◽

Vol T162 ◽

pp. 014042 ◽

Cited By ~ 9

Author(s):

L Lenhardt ◽

I Zeković ◽

T Dramićanin ◽

Ž Tešić ◽

D Milojković-Opsenica ◽

...

Keyword(s):

Support Vector Machine ◽

Infrared Spectroscopy ◽

Support Vector ◽

Support Vector Machine Algorithm ◽

Botanical Origin ◽

Unifloral Honey

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Traceability of the geographical origin of Siraitia grosvenorii based on multielement contents coupled with chemometric techniques

Scientific Reports ◽

10.1038/s41598-021-00664-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Xiao-Ping Huang ◽

Lei Lei ◽

Shun-Xin Lei ◽

Wei-Wei Zhu ◽

Jun Yan

Keyword(s):

Inductively Coupled Plasma ◽

Geographical Origin ◽

Principal Component ◽

Emission Spectrometry ◽

Support Vector ◽

Linear Discriminant ◽

Protected Designation Of Origin ◽

Siraitia Grosvenorii ◽

Discrimination Rate ◽

Chemometric Techniques

AbstractSiraitia grosvenorii (LHG) is widely used as a medicinal and edible material around the world. The objective of this study was to develop an effective method for the authentication of the geographical origin of LHG in its main producing area Guangxi, China, which is identified as Chinese Protected Designation of Origin product, against other producing regions in China. The content of 14 elements (K, Na, Ca, P, Mg, Al, B, Ba, Cu, Fe, Mn, Ni, Zn, and Sr) of 114 LHG samples was determined by inductively coupled plasma optical emission spectrometry. Multivariate analysis was then performed to classify the geographical origin of LHG samples. The contents of multielement display an obvious trend of clustering according to the geographical origin of LHG samples based on radar plot and principal component analysis. Finally, three supervised statistical techniques, including linear discriminant analysis (LDA), k-nearest neighbours (k-NN), and support vector machine (SVM), were applied to develop classification models. Finally, 40 unknown LHG samples were used to evaluate the predictive ability of model and discrimination rate of 100%, 97.5% and 100% were obtained for LDA, k-NN, and SVM, respectively. This study indicated that it is feasible to attribute unknown LHG samples to its geographical origin based on its multielement content coupled with chemometric techniques.

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Quality Assessment of Goldenrod, Milkweed and Multifloral Honeys Based on Botanical Origin, Antioxidant Capacity and Mineral Content

International Journal of Molecular Sciences ◽

10.3390/ijms23020769 ◽

2022 ◽

Vol 23 (2) ◽

pp. 769

Author(s):

Marianna Kocsis ◽

Alexandra Bodó ◽

Tamás Kőszegi ◽

Rita Csepregi ◽

Rita Filep ◽

...

Keyword(s):

Antioxidant Capacity ◽

Mineral Content ◽

Principal Component ◽

Pollen Spectrum ◽

Botanical Origin ◽

Multivariate Statistical ◽

Zn Content ◽

Five Elements ◽

Multifloral Honey ◽

Melissopalynological Analysis

The goal of the study was to evaluate the pollen spectrum, antioxidant capacity and mineral content of four Hungarian honey types, using multivariate statistical analysis. The light colored honeys were represented by milkweed honey and a multifloral (MF) honey with dominant pollen frequency of linden (MF-Tilia); the darker ones were goldenrod honey and a multifloral honey with Lamiaceae pollen majority (MF-Lamiaceae). The pollen spectrum of the samples was established with melissopalynological analysis. The absorbance of the honeys positively correlated with the antioxidant capacity determined with three of the used methods (TRC, TEAC, DPPH), but not with ORAC. The latter method correlated negatively also with other antioxidant methods and with most of the mineral values. MF-Tilia had high ORAC value, K and Na content. The MF-Lamiaceae had the highest K, Mg, P, S, Cu and Zn content, the last five elements showing strict correlation with the TRC method. The darker goldenrod honey had higher SET values and total mineral content, than the milkweed honey. The above character-sets facilitate identification of each honey type and serve as indicators of variety. The antioxidant levels and mineral content of honeys allowed their clear separation by principal component analysis (PCA).

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