Fabrication and Characterization of Gel Beads of Whey Isolate Protein–Pectin Complex for Loading Quercetin and Their Digestion Release

In this study, emulsion gel beads for loading quercetin were prepared through an emulsification/gelation process using whey protein isolate (WPI) and pectin. Emulsion gel beads’ properties were investigated by different pectin content. Additionally, the physicochemical properties, morphology and quercetin release properties from beads were explored. Firstly, electrical characteristics and the rheology of bead-forming solutions were measured, revealing that all systems had strong negative charge and exhibited shear-thinning behavior. The textural results demonstrated that the properties of emulsion gel beads were improved with increasing the content of pectin. It was also confirmed that crosslinking was formed between WPI emulsion and pectin by Fourier Transform Infrared (FTIR) analysis and thermogravimetric analysis (TGA). In addition, the shape of the beads was spherical or ellipses with smooth surfaces and they had a tight gel network of internal structures, which was visualized by using electron microscopy (SEM). Finally, the amount of quercetin released in vitro was gradually decreased with increasing pectin content; it was as low as 0.59%. These results revealed that WPI emulsion–pectin gel beads might be an effective delivery system for quercetin as a colon target and are worth exploring further.

Download Full-text

Effect of structuring emulsion gels by whey or soy protein isolate on the structure, mechanical properties, and in-vitro digestion of alginate-based emulsion gel beads

Food Hydrocolloids ◽

10.1016/j.foodhyd.2020.106165 ◽

2021 ◽

Vol 110 ◽

pp. 106165 ◽

Cited By ~ 2

Author(s):

Duanquan Lin ◽

Alan L. Kelly ◽

Valentyn Maidannyk ◽

Song Miao

Keyword(s):

Mechanical Properties ◽

Soy Protein ◽

Soy Protein Isolate ◽

In Vitro Digestion ◽

Protein Isolate ◽

Emulsion Gel ◽

Gel Beads

Download Full-text

Identification and Characterization of Novel Antioxidant Protein Hydrolysates from Kiwicha (Amaranthus caudatus L.)

Antioxidants ◽

10.3390/antiox10050645 ◽

2021 ◽

Vol 10 (5) ◽

pp. 645

Author(s):

Sergio Montserrat-de la Paz ◽

Alicia Martinez-Lopez ◽

Alvaro Villanueva-Lazo ◽

Justo Pedroche ◽

Francisco Millan ◽

...

Keyword(s):

Antioxidant Capacity ◽

Reducing Power ◽

Protein Isolate ◽

Protein Hydrolysates ◽

Antioxidant Peptides ◽

Amaranthus Caudatus ◽

Intact Proteins ◽

Identification And Characterization

Kiwicha (Amaranthus caudatus) is considered one of the few multipurpose pseudocereals for its potential use not only as a source of nutrients and fiber but also for its bioactive compounds. In recent years, antioxidant peptides are commonly used as functional ingredient of food. Herein, a kiwicha protein isolate (KPI), obtained from kiwicha defatted flour (KDF), was hydrolyzed by Bioprotease LA 660, a food-grade endoprotease, under specific conditions. The resulting kiwicha protein hydrolysates (KPHs) were chemically characterized and their digestibility and antioxidant capacity were evaluated by in vitro cell-free experiments owing to their measure of capacity to sequester DPPH free radical and reducing power. KPHs showed higher digestibility and antioxidant capacity than intact proteins into KPI. Therefore, the results shown in this study indicate that KPHs could serve as an adequate source of antioxidant peptides, representing an effective alternative to the generation of functional food.

Download Full-text

The impact of pH on mechanical properties, storage stability and digestion of alginate-based and soy protein isolate-stabilized emulsion gel beads with encapsulated lycopene

Food Chemistry ◽

10.1016/j.foodchem.2021.131262 ◽

2021 ◽

pp. 131262

Author(s):

Duanquan Lin ◽

Alan L. Kelly ◽

Song Miao

Keyword(s):

Mechanical Properties ◽

Soy Protein ◽

Storage Stability ◽

Soy Protein Isolate ◽

Protein Isolate ◽

Emulsion Gel ◽

Gel Beads ◽

The Impact

Download Full-text

Organic selenium derived from chelation of soybean peptide-selenium and its functional properties in vitro and in vivo

Food & Function ◽

10.1039/c9fo00729f ◽

2019 ◽

Vol 10 (8) ◽

pp. 4761-4770 ◽

Cited By ~ 9

Author(s):

Qianwen Ye ◽

Xiaoping Wu ◽

Xinyuan Zhang ◽

Shaoyun Wang

Keyword(s):

Antioxidant Activity ◽

Functional Properties ◽

Soybean Protein ◽

Protein Isolate ◽

Soybean Protein Isolate ◽

Organic Selenium

The preparation and characterization of a soybean protein isolate peptide-Se chelate with remarkably antioxidant activity in vitro and in vivo.

Download Full-text

Extended Pharmacopeial Characterization of Surfactant Aerosols Generated by a Customized eFlow Neos Nebulizer Delivered through Neonatal Nasal Prongs

Pharmaceutics ◽

10.3390/pharmaceutics12040319 ◽

2020 ◽

Vol 12 (4) ◽

pp. 319 ◽

Cited By ~ 2

Author(s):

Federico Bianco ◽

Elena Pasini ◽

Marcello Nutini ◽

Xabier Murgia ◽

Carolin Stoeckl ◽

...

Keyword(s):

Aerodynamic Characteristics ◽

Lung Deposition ◽

Lung Dose ◽

Nasal Prong ◽

Upper Airways ◽

Nominal Dose ◽

Effective Delivery ◽

Preclinical And Clinical Studies

The delivery of nebulized medications to preterm infants during Non-Invasive Ventilation (NIV) remains an unmet clinical need. In this regard, the effective delivery of nebulized surfactant has been particularly investigated in preclinical and clinical studies. In this work, we investigated the feasibility of delivering nebulized surfactant through various commercially available nasal prong types. We first performed a compendial characterization of surfactant aerosols generated by the eFlow Neos nebulizer, customized to be used in neonates, determining the amount of surfactant delivered by the device as well as the aerodynamic characteristics of surfactant aerosols. Additionally, we extended the compendial characterization by testing the effect of different nasal prong types on the estimated lung dose using a realistic Continuous Positive Airway Pressure (CPAP) circuit that included a cast of the upper airways of a preterm neonate. The compendial characterization of surfactant aerosols delivered through different nasal prongs achieved relatively high delivered surfactant doses (in the range 63–74% of the nominal dose), with aerodynamic characteristics displaying mass median aerodynamic diameters ranging between 2.52 and 2.81 µm. Nevertheless, when using a representative in vitro setup mimicking NIV in a clinical setting, significant differences were observed in terms of the estimated lung dose accounting for up to two-fold differences (from 10% to 20% estimated lung deposition of the nominal dose) depending on the chosen nasal prong type. Considering that surfactant lung deposition rates are correlated with therapeutic efficacy, this study points out the relevance of choosing the appropriate NIV interface to maximize the lung dose of nebulized medications.

Download Full-text

Physicochemical and Functional Characterization of Newly Designed Biopolymeric-Based Encapsulates with Probiotic Culture and Charantin

Foods ◽

10.3390/foods10112677 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2677

Author(s):

Awa Fanny Massounga Bora ◽

Xiaodong Li ◽

Lu Liu

Keyword(s):

Inhibitory Activity ◽

Functional Characterization ◽

Surface Hydrophobicity ◽

Protein Isolate ◽

Viable Count ◽

Scanning Calorimetry ◽

Single Carrier ◽

Carrier Materials

The identification of novel sources of synbiotic agents with desirable functionality is an emerging concept. In the present study, novel encapsulates containing probiotic L. acidophilus LA-05® (LA) and Charantin (CT) were produced by freeze-drying technique using pure Whey Protein Isolate (WPI), pure Maltodextrin (MD), and their combination (WPI + MD) in 1:1 core ratio, respectively. The obtained microparticles, namely WPI + LA + CT, MD + LA + CT, and WPI + MD + LA + CT were tested for their physicochemical properties. Among all formulations, combined carriers (WPI + MD) exhibited the highest encapsulation yields for LA (98%) and CT (75%). Microparticles showed a mean d (4, 3) ranging from 50.393 ± 1.26 to 68.412 ± 3.22 μm. The Scanning Electron Microscopy revealed uniformly amorphous and glass-like structures, with a noticeably reduced porosity when materials were combined. In addition, Fourier Transform Infrared spectroscopy highlighted the formation of strong hydrogen bonds supporting the interactions between the carrier materials (WPI and MD) and CT. In addition, the thermal stability of the combined WPI + MD was superior to that of pure WPI and pure MD, as depicted by the Thermogravimetric and Differential Scanning Calorimetry analysis. More interestingly, co-encapsulation with CT enhanced LA viability (8.91 ± 0.3 log CFU/g) and Cells Surface Hydrophobicity (82%) in vitro, in a prebiotic-like manner. Correspondingly, CT content was heightened when co-encapsulated with LA. Besides, WPI + MD + LA + CT microparticles exhibited higher antioxidant activity (79%), α-amylase inhibitory activity (83%), and lipase inhibitory activity (68%) than single carrier ones. Furthermore, LA viable count (7.95 ± 0.1 log CFU/g) and CT content (78%) were the highest in the blended carrier materials after 30 days of storage at 4 °C. Synbiotic microparticle WPI + MD + LA + CT represents an effective and promising approach for the co-delivery of probiotic culture and bioactive compounds in the digestive tract, with enhanced functionality and storage properties.

Download Full-text