scholarly journals Impact of Trichoderma asperellum on Chilling and Drought Stress in Tomato (Solanum lycopersicum)

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 385
Author(s):  
Karen Cornejo-Ríos ◽  
María del Pilar Osorno-Suárez ◽  
Sergio Hernández-León ◽  
Ma Isabel Reyes-Santamaría ◽  
Javier Andrés Juárez-Díaz ◽  
...  

The acceleration of climate change is necessitating the adoption of shifts in farming practices and technology to ensure the sustainability of agricultural production and food security. Because abiotic stresses such as drought and chilling represent major constraints on agricultural productivity worldwide, in this study, the mitigation of such stresses by the fungus Trichoderma asperellum HK703 was evaluated. The fungus was grown on whole grain oats, kaolin and vermiculite for 5 days and then the formulation was mixed with the potting soil to colonize the roots of the plants. The effect of the bioinoculant on tomato under drought or chilling was analyzed in tomato (Solanum lycopersicum) plants. Leaf, stem and root succulence, electrolyte leakage, the relative growth rate of plant height, stem thickness and leaf area, as well new leaf emergence and chlorophyll content were determined. The results showed that drought or chilling increased electrolyte leakage and reduced plant growth and development traits and chlorophyll (a,b) content. However, inoculation with T. asperellum eliminated or reduced most of the negative impacts of drought compared to the non-stressed plants, with the exception of chlorophyll b content. Furthermore, inoculation with T. asperellum improved some of the evaluated features in chilling stressed plants but had no effect on plant height or chlorophyll (a,b) content. The results of this study indicate that T. asperellum was more effective in alleviating drought than chilling stress in tomato plants.

2016 ◽  
Vol 24 (1) ◽  
pp. 42-47 ◽  
Author(s):  
Matúš Peško ◽  
Marianna Molnárová ◽  
Agáta Fargašová

AbstractPresented study evaluates effects of various Sb(III) concentrations on tomato plants (Solanum lycopersicum) cultivated hydroponically. Visual symptoms of antimony toxicity were observed only at two highest applied concentrations (50 and 100 mg/L). Dry weight of aboveground parts decreased significantly in variants treated with 25, 50 and 100 mg/L Sb(III), by ~12, 35 and 65 %, respectively, in comparison to the control. Statistically significant decrease of chlorophyll a and b was observed only after application of two highest studied concentrations 50 and 100 mg/L Sb(III). On the other hand concentration of total carotenoids in leaves rose with increasing external Sb(III) concentration. High concentrations (50 and 100 mg/L) of Sb(III) in nutrient solution caused that protein content in leaves dropped by ~20 and 39% relative to control. Accumulation of antimony in roots was about 5- (10 mg/L) to 27-times (25 mg/L) greater than that in shoots. The highest BAF factor value determined for shoots was ~55 at 10 mg/L Sb(III) and for roots it was ~821 at 50 mg/L Sb(III). Translocation factor values were in whole studied concentration range 5 – 100 mg/L Sb(III) < 1. The most effective translocation of antimony from roots to shoots was observes for variants treated with 10 mg/L of Sb(III).


Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 510
Author(s):  
Khaled Abdelaal ◽  
Kotb A. Attia ◽  
Gniewko Niedbała ◽  
Tomasz Wojciechowski ◽  
Yaser Hafez ◽  
...  

Garlic is an important vegetable in terms of its economic value and also as a medicinal plant. In this study, chitosan (300 mM) and yeast extract (8 g/L) were used individually or in combination to improve the yields of garlic plants under drought conditions (i.e., 75% and 50% of the water they would normally receive from irrigation) for two seasons. Significant decreases in numbers of leaves per plant and plant height, plant dry weight, relative water content, and chlorophyll a and b concentrations were found in stressed garlic plants in both seasons. The greatest reductions in these characters were recorded in plants that received only 50% of the normal irrigation in both seasons. Levels of hydrogen peroxide, products of lipid peroxidation such as malondialdehyde, and superoxide, as well as percentages of electrolyte leakage, were elevated considerably and were signals of oxidative damage. The application of the yeast extract (8 g/L) or chitosan (300 mM) individually or in combination led to a remarkable increase in the most studied characters of the stressed garlic plants. The combination of yeast extract (8 g/L) plus chitosan (300 mM) led to increase plant height (44%), ascorbic acid levels (30.2%), and relative water content (36.8%), as well as the chlorophyll a (50.7%) and b concentrations (79%), regulated the proline content and levels of antioxidant enzymes in stressed garlic plants that received 75% of the normal irrigation, and this decreased the signs of oxidative stress (i.e., percentage of electrolyte leakage and levels of malondialdehyde, hydrogen peroxide, and superoxide).


Author(s):  
Agus Suyanto ◽  
Agnes Tutik Purwani Irianti ◽  
Hamdani Hamdani ◽  
Ismail Astar ◽  
Dwi Nurteto

<em>This study aims to determine the interaction effect of the type and dose of Trichoderma sp. and its secondary metabolites on suppression of Fusarium sp. wilt disease and its effect on the growth of tomato plants (Solanum lycopersicum mill.). This study used a Randomized Block Design (RBD) which consisted of two factors. The first factor is the type of application (J) which consists of three levels of treatment, namely: J1 = Trichoderma sp., J2 = Secondary metabolites. J3 = Trichoderma sp. + Secondary metabolites, while the second factor is the application dose (D) which consists of three treatment levels, namely: D1 = 10 ml/plant, D2 = 20 ml/plant, D3 = 30 ml/plant. The treatment was repeated 3 times to obtain 81 tomato plants and added 9 control treatment plants. The parameters observed were the percentage of wilt disease, plant height, stem diameter, number of leaflets, and number of branches. The results showed that in laboratory testing, the mushrooms Trichoderma sp. and secondary metabolites can inhibit the growth of the mushrooms Fusarium sp. In research in the field, treatment with Trichoderma sp., secondary metabolites, and Trichoderma sp. + secondary metabolites, with various spore densities and applied 7 days before planting can reduce the wilting percentage to 0%. The interaction treatment of type and application dose had a very significant effect on the number of leaflets, and had a significant effect on stem diameter but had no significant effect on plant height and number of branches. The highest results on the variable plant height and number of leaflets were found in the J3D3 treatment with a height of 82.67 cm and a number of leaflets of 85.11 strands. The highest results on the stem diameter variable were found in the J1D3 treatment with a diameter of 6.59 mm, while the variable number of branches was found in the J3D1 treatment with 1.35 branches.</em>


2015 ◽  
Vol 25 (4) ◽  
pp. 522-527 ◽  
Author(s):  
Lucia E. Villavicencio ◽  
James A. Bethke ◽  
Lea Corkidi

Two experiments were conducted to evaluate the effect of the plant regulator uniconazole on plant height, flowering, and fruit yield of vegetable transplants. In the first experiment, seedlings of tomato (Solanum lycopersicum ‘Early Girl’), pepper (Capsicum annuum ‘Jalapeno’), and eggplant (Solanum melongena ‘Millionaire’), were sprayed with water (untreated control) or with 2.5, 5, and 10 mg·L−1 of uniconazole. Five weeks after treatment (WAT), application of 2.5 mg·L−1 of uniconazole reduced the height of tomato by 17%, and of 5 and 10 mg·L−1, by 25%. The effect of 10 mg·L−1 of uniconazole on tomato plant height persisted until 13 WAT, but did not affect fruit yield. ‘Early Girl’ tomato plants treated with 10 mg·L−1 of uniconazole were still shorter than the untreated control at this time, but there were no significant differences in the number or weight of the fruit produced by the plants treated with 10 mg·L−1 of uniconazole, and the untreated controls. In contrast, as the rate of uniconazole increased, the height of ‘Jalapeno’ pepper and ‘Millionaire’ eggplant decreased. Application of uniconazole had no effect on the number of fruit produced by ‘Millionaire’ eggplant. However, treatment with 10 mg·L−1 of uniconazole reduced the number of fruit produced by pepper plants by 50%, and reduced the total weight of fruit produced by pepper and eggplant plants by 30% and 50%, respectively, compared with the untreated control. The second experiment analyzed the effects of 5, 8, and 10 mg·L−1 of uniconazole on two cultivars of tomato with different growth habit, Early Girl (determinate) and Sun Sugar (indeterminate). Application of all rates of uniconazole decreased plant height but not the final fruit yield of the two tomato cultivars.


Plant Disease ◽  
2014 ◽  
Vol 98 (5) ◽  
pp. 698-698 ◽  
Author(s):  
Y. Tomitaka ◽  
T. Usugi ◽  
R. Kozuka ◽  
S. Tsuda

In 2009, some commercially grown tomato (Solanum lycopersicum) plants in Chiba Prefecture, Japan, exhibited mosaic symptoms. Ten plants from a total of about 72,000 cultivated plants in the greenhouses showed such symptoms. To identify the causal agent, sap from leaves of the diseased plants was inoculated into Chenopodium quinoa and Nicotiana benthamiana plants. Local necrotic lesions appeared on inoculated leaves of C. quinoa, but no systemic infection was observed. Systemic mosaic symptoms were observed on the N. benthamiana plants inoculated. Single local lesion isolation was performed three times using C. quinoa to obtain a reference isolate for further characterization. N. benthamiana was used for propagation of the isolate. Sap from infected leaves of N. benthamiana was mechanically inoculated into three individual S. lycopersicum cv. Momotaro. Symptoms appearing on inoculated tomatoes were indistinguishable from those of diseased tomato plants found initially in the greenhouse. Flexuous, filamentous particles, ~750 nm long, were observed by electron microscopy in the sap of the tomato plants inoculated with the isolate, indicating that the infecting virus may belong to the family Potyviridae. To determine genomic sequence of the virus, RT-PCR was performed. Total RNA was extracted from the tomato leaves experimentally infected with the isolate using an RNeasy Plant Mini kit (QIAGEN, Hilden, Germany). RT-PCR was performed by using a set of universal, degenerate primers for Potyviruses as previously reported (2). Amplicons (~1,500 bp) generated by RT-PCR were extracted from the gels using the QIAquick Gel Extraction kit (QIAGEN) and cloned into pCR-BluntII TOPO (Invitrogen, San Diego, CA). DNA sequences of three individual clones were determined using a combination of plasmid and virus-specific primers, showing that identity among three clones was 99.8%. A consensus nucleotide sequence of the isolate was deposited in GenBank (AB823816). BLASTn analysis of the nucleotide sequence determined showed 99% identity with a partial sequence in the NIb/coat protein (CP) region of Colombian datura virus (CDV) tobacco isolate (JQ801448). Comparison of the amino acid sequence predicted for the CP with previously reported sequences for CDV (AY621656, AJ237923, EU571230, AM113759, AM113754, and AM113761) showed 97 to 100% identity range. Subsequently, CDV infection in both the original and experimentally inoculated plants was confirmed by RT-PCR using CDV-specific primers (CDVv and CDVvc; [1]), and, hence, the causal agent of the tomato disease observed in greenhouse tomatoes was proved to be CDV. The first case of CDV on tomato was reported in Netherlands (3), indicating that CDV was transmitted by aphids from CDV-infected Brugmansia plants cultivated in the same greenhouse. We carefully investigated whether Brugmansia plants naturally grew around the greenhouses, but we could not find them inside or in proximity to the greenhouses. Therefore, sources of CDV inoculum in Japan are still unclear. This is the first report of a mosaic disease caused by CDV on commercially cultivated S. lycopersicum in Japan. References: (1) D. O. Chellemi et al. Plant Dis. 95:755, 2011. (2) J. Chen et al. Arch. Virol. 146:757, 2001. (3) J. Th. J. Verhoeven et al. Eur. J. Plant. Pathol. 102:895, 1996.


AGRIFOR ◽  
2018 ◽  
Vol 17 (2) ◽  
pp. 231
Author(s):  
Dian Kristina ◽  
Abdul Rahmi

This experiment aims to: (1) to study of the effect of guano walet fertilizer and Ratu Biogen foliar fertilizeras well as their interaction on the growth and yield of tomato plants; and (2) to find proper dosage of guano walet fertilizer and proper concentration of Ratu Biogen foliar fertilizer for better growth and yield of tomato plants.The research carried out from May 2014 to July 2014, in the Village Melak Ulu RT.20 Subdistrict Melak, West Kutai. It applied Completely Randomized Design with factorial experiment 4 x 4 and five replications.  The first factor is the dosage of the guano walet fertilizer (G) consists of 4 levels, namely: no fertilizer application guano walet (g0), 10 Mg ha ̵ ¹, or 100 g of polybag ̵ ¹ (g1), 15 Mg ha ̵ ¹ or 150 g polibag ̵ ¹ (g2), 20 Mg ha ̵ ¹ or 200 g polybag ̵ ¹   (g3). The second factor is the concentration of Ratu Biogen (B) consists of 4 levels: without POC Ratu Biogen (b0), 1 ml 1 ̵ ¹ water (b1), 2 ml 1 ̵ ¹ water (b2), 3 ml 1 ̵ ¹  water (b3).Result of the research revealed that : (1) application of guano walet fertilizer affect very significantly on plant height at 14, 28, 42 days after planting, the number of fruits per plant, and weight of fruit per plant, but the effect is not significant on the days of plant flowered and days of plant harvest.  The best production is attained by the 200 g polybag-1 fertilizer guano walet (g3), namely 282,50 plant-1, In reverse, the least production is attained by without fertilizer guano walet (g0), namely 227,25 g plant ̵ ¹; (2) application of Ratu Biogen foliar fertilizer after significantly to very significantly on the plant height at 14 days after planting  and the number of fruits per plant, but the effect is no significant on the plant height at 28 and 42 days after planting, days of plant flowered, days of plant harvest, and weight of fruit per plant; and (3) interaction between guano walet fertilizer and Ratu Biogen foliar fertilizer no significantly on the plant height at 14, 28, and 42 days after planting, days of plant flowered, days of plant harvest, number of fruit per plant, and fruit weight per plant.


2021 ◽  
Vol 68 (5) ◽  
pp. 923-930
Author(s):  
Z. R. Vershinina ◽  
O. V. Chubukova ◽  
D. R. Maslennikova

Abstract The level of glutathione was investigated in the roots of tomato (Solanum lycopersicum L.) plants transgenic by genes psl and rapA1 in the presence of a microsymbiont of leguminous plants Rhizobium leguminosarum VSy3. The plants transformed with gene psl showed a greater bacterial adhesion than the plants transformed with gene rapA1, which positively correlated with growth parameters of plants. Treatment with rhizobia elevated the content of glutathione in the roots of wild type plants three times, 4.7 times in the roots of plants transformed with gene rapA1, and more than five times in the plants transgenic by gene psl. The obtained results suggest that the level of glutathione in the roots may serve as a marker of efficiency of artificial symbiotic systems produced de novo.


Author(s):  
V. Sievidov ◽  
◽  
I. Sievidov ◽  

One of the main factors in obtaining consistently high tomato yields is to optimize the plant nutrition area. Determination of the optimal plant density, on the one hand, prevents oppression of plants at increased density. On the other hand, to avoid unnecessary expenses from the irrational use of the cultivated area. Vegetables are one of the main suppliers of biologically active substances necessary for a good human nutrition. They give the body a lot of vitamins, fiber, hemicelluloses, pectin substances, organic acids, various carbohydrates, mineral salts and a number of other biochemical compounds. Tomato is one of the main protected ground crops for Ukraine. Compared to other crops, tomatoes give early and stable yields. The issue of planting density of tomatoes is still not fully resolved, these elements of technology are not adapted to the soil and climatic conditions of the eastern part of the Left-Bank Forest-Steppe of Ukraine. The objective of our research was to determine the optimal crop density of hybrid tomato of indeterminate type in order to obtain the highest yield without reducing the quality of the product. The method of research. The research was carried out during 2018-2019. In film greenhouses, spring-summer crop rotation. The experiments were carried out with an indeterminate tomato hybrid: Tobolsk F1. Producers of seeds of indeterminate hybrids recommend different plant densities for growing conditions in film greenhouses 2.5-3.5 pcs/m2. Therefore, our research was planned to determine the optimal plant density of the indeterminate tomato hybrid Tobolsk F1 for film greenhouses. The total number of plants is 312 pcs. Sowing of seeds was carried out in the third decade of February. The seeds were sown into cassettes, and the seedlings were dived into pots (volume - 500 cm3) on time. Seedlings were grown using bottom irrigation and, at the age of 3-5 true leaves, the seedlings were planted on a test plot in a film greenhouse without heating. Research results. An analysis of phenological observations of plants showed that a change in the density of plants had practically no effect on the timing and rate of passage of the stages of organogenesis in plants, that is, in all variants of the experiment, the phases of development in plants began simultaneously. Indicators of plant parameters indicate that the data obtained both in the phase of mass flowering and mass fruiting of tomatoes differ among themselves. The difference in biometric parameters can be traced depending on the density of plants. Comparing the main biometric indicators, it can be noted that in the flowering phase, the height of plants ranged from 111.0 to 134.9 cm, in the fruiting phase - from 257.0 to 275.8 cm, while the plants differed in height by the density of 4.0 pcs/m2. The vegetative mass of a plant in the flowering phase was from 1884 g with a plant density of 2.5 pcs/m2 to 1144 g with a density of 4.0 pcs/m2. In the phase of flowering fruiting, the weight of the plant ranged from 1704 g to 1574 g, also decreasing with increasing density. In the flowering phase, an increase in the value of the leaf area indicator was observed to 5.8% with an increase in plant density, and in the fruiting phase, a slight decrease in the indicator to -1.8% was observed with an increase in plant density. So, according to biometric indicators, plants develop better with a density of 3.5 pcs/m2: tomato plants have the best indicators of vegetative mass and plant height, the leaf surface area varies within insignificant limits. The size of the fruits and the yield of standard tomato production are in direct proportion to the density of plants, that is, the more of them per unit area, the lower these indicators. In general, the increase in the density of tomato plants significantly affected the yield. Conclusions. Two-year researches have established that with an increase in plant density, in terms of leaf area in tomato plants of the Tobolsk F1 hybrid, on average, there was a slight fluctuation in the indicator at the level of 0.9-1.1%. The indicator of the vegetative mass of the plant ranged from -4.1 to +1.8% as compared to the control, also decreasing with increasing density. The indicator of plant height both in the flowering phase and in the fruiting phase, on the contrary, grew with an increase in plant density and ranged from -4.0 to + 7.1% compared to the control, while the plants differed in height by a density of 4.0 pcs/m2. In general, the studies carried out give grounds to conclude that in a spring film greenhouse, according to biometric indicators, on average, plants develop better with a density of 3.5 pcs/m2: tomato plants have the best ratio of vegetative mass, plant height and leaf area. The maximum yield of tomato hybrid Tobolsk F1 at the level of 15.8 kg/m2 in the eastern part of the Left-Bank Forest-Steppe of Ukraine was obtained with a plant density of 3.5 pcs/m2.


2021 ◽  
Vol 35 (2) ◽  
Author(s):  
Amine Khoulati ◽  
E. Saalaoui

An experiment was carried out in a greenhouse to study the effect of aqueous extracts of Crocus sativus L. by-products on tomato plants. Three concentrations of tepals and corms were used by fertigation: 1 g/L, 2 g/L, and 3 g/L. The aqueous extract of the stigmas was used as a foliar application at 0.6 g/L. The experiment was carried out in a completely randomized block with three repetitions for each concentration. The concentration of tepal extract at 3 g/L significantly (p≤0.05) increased the plants' height, the chlorophyll a, b content. The same results were observed for the foliar treatment with stigmas; however, there was no effect of tepal extract on the carotenoid content. On the other hand, the concentration 2 g/L of the corms extract had a positive impact (p≤0.05) in the chlorophyll b content while the concentration of 3 g/L increased the plant's height, the chlorophyll a (p≤0.05). Current results indicate that Crocus sativus by-products could improve certain physiological aspects of the recipient plants and new and natural biostimulants.


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