scholarly journals Heterosis and Combining Ability for Fruit Yield, Sweetness, β-Carotene, Ascorbic Acid, Firmness and Fusarium Wilt Resistance in Muskmelon (Cucumis melo L.) Involving Genetic Male Sterile Lines

Horticulturae ◽  
2022 ◽  
Vol 8 (1) ◽  
pp. 82
Author(s):  
Simranpreet Kaur ◽  
Sat Pal Sharma ◽  
Navraj Kaur Sarao ◽  
Jaideep Kaur Deol ◽  
Rupeet Gill ◽  
...  

Ten genetically diverse inbred lines, including two genic male sterile lines, of muskmelon (Cucumis melo L.) were crossed in a half-diallel to generate 45 F1 hybrids. These hybrids, along with the parental lines and commercial check, were evaluated for their fruit yield, level of phytochemicals and Fusarium wilt resistance. Both additive and non-additive genetic variances were important in governing the expression of all of the traits; however, the additive gene action for the fruit weight (g), flesh thickness (cm), rind thickness (mm), firmness (lb inch−2), β-carotene content (mg/100 g), non-additive variance for fruit yield (t ha−1), fruit number, total soluble solids (TSS, °Brix), ascorbic acid (mg/100 g) and reaction to Fusarium wilt were comparatively more important. The parental line MM-625 was the best general combiner for fruit yield, rind thickness and β-carotene content (mg/100 g). The exotic line Riogold was the best combiner for flesh thickness and firmness. The netted inbred line MM-610 was the best general combiner for fruit weight, ascorbic acid and reaction to Fusarium wilt. The inbred lines KP4HM-15 and MM-916 were the best general combiners for the number of fruits per vine and TSS. The best cross-combinations for fruit yield ha−1 and TSS were MS-1×M-610 and Kajri×MM-904, respectively. The hybrids KP4HM-15×MM Sel-103 and KP4HM-15×MM-1831 recorded the highest standard heterosis for fruit yield and TSS. The landrace-derived inbred lines Kajri, MM Sel-103 and KP4HM-15 produced moderate-to-highly FW-resistant hybrids. Out of the 121 SSR markers applied, 70 exhibited parental polymorphism. The markers DM0561, CMAAAGN14, TJ147, CMMS35_3, CMAGN45 and DE1337 identified specific/unique alleles in certain parental genotypes. Thus, the findings of this study revealed that the novel inbred lines can effectively be combined to generate heterotic F1 hybrids for yield and other traits, such as rind and flesh thickness, TSS, β-carotene content and firmness. Furthermore, SSR markers can potentially be utilized to confirm the genetic diversity among the parental lines, and for the DNA fingerprinting of F1 hybrids.

2012 ◽  
Vol 12 (51) ◽  
pp. 6186-6199
Author(s):  
TA Jahan ◽  
◽  
AKMA Islam ◽  
MG Rasul ◽  
MAK Mian ◽  
...  

The heterotic effects and genetic components of variation for qualitative and quantitative characters were estimated in sweet gourd. The phenotypic coefficients of variation were higher than genotypic coefficient of variation for all the characters indicating that environment played a considerable role on the expression of these characters. High heritability coupled with high genetic advance found in parents and hybrids for number of fruits per plant, individual fruit weight and fruit yield suggested that improvement would be effective through phenotypic selection. Both positive and negative heterosis was observed for different qualitative and quantitative characters in F1 hybrids of sweet gourd. None of the hybrids exhibited maximum heterosis for all the traits, but significant and desirable level of heterosis over mid parent and better parent was obtained in several hybrids for the different traits. For node number of first female flower, two hybrids, OP 10×OP 24 and OP 10×OP 20 recorded the highest significant positive mid and better parent heterosis, respectively. The highest significant (p<0.05) positive mid and better parent heterosis for female flowers per plant was exhibited by the hybrid OP 20×OP 02. Significant (p<0.05) and desirable level of mid and better parent heterosis was exhibited by six and four hybrids, respectively, for fruits per plant and fruit yield per plant. The hybrids OP 10×OP 20 and OP 20×OP 02 showed the highest significant (p<0.05) positive mid parent and better parent heterosis for % reducing sugar, respectively. None of the hybrids showed significant (p>0.05) heterosis over mid and better parent for brix content. The hybrids OP 10×OP 02 and OP 04×OP 02 showed significant mid parent heterosis for carotene content. Four hybrids, OP 10×OP 20, OP 20×OP 02, OP 10×OP 02 and OP 04×OP 02 exhibited significant (p<0.05) positive heterosis for majority of the characters studied and were identified as promising for commercial cultivation. We concluded that performance of these hybrids needs to be further evaluated in multi location or on farm trial prior to commercial use.


Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 692 ◽  
Author(s):  
Aleck Kondwakwenda ◽  
Julia Sibiya ◽  
Rebecca Zengeni ◽  
Cousin Musvosvi ◽  
Samson Tesfay

Provitamin A maize (Zea mays L.) biofortification is an ideal complementary means of combating vitamin A deficiency (VAD) in sub-Saharan Africa where maize consumption is high coupled by high VAD incidences. However, drought remains a major abiotic constraint to maize productivity in this region. Comprehensive drought screening of initial breeding materials before advancing them is important to achieve genetic gain. In this study, 46 provitamin-A inbred lines were screened for drought tolerance in the greenhouse and field under drought and optimum conditions using β-carotene content (BCC), grain yield (GY), and selected morphophysiological and biochemical traits. The results revealed that BCC, morphophysiological and biochemical traits were effective in discriminating among genotypes. Number of ears per plant (EPP), stomatal conductance (Gs), delayed leaf senescence (SEN), leaf rolling (RL), chlorophyll content (CC) and free proline content (PC) proved to be ideal traits to use when indirectly selecting for GY by virtue of having relative efficiency of indirect selection values that are greater than unity and considerable genetic variances under either or both conditions. The findings of this study form the basis of initial germplasm selection when improving provitamin A maize for drought tolerance.


Author(s):  
Anuradha Sinha ◽  
Paramveer Singh ◽  
Ajay Bhardwaj ◽  
R. B. Verma

Yield and yield contributing attributes are the mostly targeted in tomato improvement programme so the existence of variability has prime importance in plant breeding. In this study 14 genotypes were assessed with principal component analyses (PCA) based on morphological and biochemical traits to select genotypes and traits for future breeding program. Based on the PCA with 21 traits, 14 components were formed however, 6 PCs had more than 1 Eigen value with the variability of 87.60%. So, these six PC were used for further explanation. The first principal component explained maximum variability of the total variation presented. PC1 correlated with days to first flowering, days to 50% flowering and days to first fruiting etc. while PC2 dominated by β- carotene, lycopene, total soluble solid and ascorbic acid. PC 3 correlated with the traits like plant height at final harvest, number of fruits per plant and days to 50% flowering. PC 4 reflected positive factor loading by polar diameter, days taken to first picking and plant height at final harvest. PC 5 correlated with polar diameter, node to first flowering and ascorbic acid with positive factor loading and PC 6 correlated with ascorbic acid, days to 50% flowering, number of fruits per truss, equatorial diameter, pericarp thickness, titratable acidity, β-carotene, average fruit weight, fruit yield per plant. Therefore, the important traits coming jointly from diverse PCs and contributing towards elucidation variability may be kept into consideration during utilization of these traits in breeding programme of tomato.


Author(s):  
W.S.D. Yamika ◽  
G.P. Simbolon ◽  
B. Waluyo ◽  
N. Aini

Background: Cutleaf groundcherry (Physalis angulata L.) has vitamins that are good for health. There were several genotypes of cutleaf groundcherry that have the potential to be widely cultivation, but information of doses of NPK fertilizer for those genotypes was still limited. The objective of this experiment was to determine the response of cutleaf groundcherry to different rates of NPK fertilizer. Methods: The experiment was conducted May - September 2019 in dry land field. The experiment used a factorial randomized block design with three replications. The first factor was genotypes cutleaf groundcherry (PA 01, PA 02 and PA 03) and the second factor was doses of NPK fertilizer (75, 150 and 225 kg ha-1 NPK). Conclusion: The increased in doses of NPK fertilizer to 225 kg ha-1 NPK increased nutrient uptake, plant growth and fresh fruit weight for each genotype of cutleaf groundcherry. Meanwhile ascorbic acid content increased with a dose of 150 kg ha-1 NPK, but decreased with increased NPK fertilizer rates. The beta-carotene content with 75 kg ha-1 NPK fertilizer was higher than with 150 and 225 kg ha-1 doses. The increase of NPK fertilizer doses otherwise decreases the beta-carotene content for each genotype. Shoot dry weight, fresh fruits weight and ascorbic acid in PA 03 genotype higher than PA 01 and PA 02 genotypes.


2020 ◽  
pp. 141-152
Author(s):  
Desalegn Negasa Soresa ◽  
Gomathi Nayagam ◽  
Netsanet Bacha ◽  
Zerihun Jaleta

Estimates of heterosis for F1 hybrids over mid and better parent were computed for traits that showed significant differences between genotypes on analysis of variance. Heterosis for yield components and yield was studied using 8x8 half diallel cross in tomato (Solanum lycopersicum L.). The heterosis for yield was generally accompanied by heterosis for yield components. Heterosis for marketable fruit yield per plant ranged from (-63.4%) (P3xP8) to (33.8%) (P6xP8) and (-62.5%) (P3xP8) to (52.6%) (P5xP7), for mid parent and better parent respectively. Significant heterosis over better and mid-parent was observed for all the traits.  Best parent and Mid-parent heterosis (MPH) was highest  and in desirable direction for number of marketable fruit per plant  (29.3%; 29.2%)  in crosses ( P3xP6 for both ) and pericarp thickness  (46.3%; 57.6%) in crosses (P2xP6 and P4xP8), number of fruit cluster per plant (32.8%; 35.9%) in cross (P3xP6 for both), individual fruit weight (36.1%; 41.2%) in cross (P2xP8, P3xP5) and fruit diameter (28.4%; 28.3%) in cross (P3xP5; P2xP6), fruit length (23.07%; 20.4%)  in cross (P2xP6 for both). Out of 28 F1 crosses, positive and desirable heterosis by 10 crosses over better parent and 17 crosses over mid-parent were observed for total fruit density in tomato. An important heterosis both in heterobeltiosis and mid-parent was recorded in marketable fruit yield in ton per hectare. From all the crosses, seven crosses revealed positive from which three crosses are the most important P2xP7 (31%),  P3xP5 (20%) and P3xP6 (54%) in better parent heterosis. Similarly for mid-parent heterosis, only ten crosses out of 28 reveled positive while the rest 18 crosses showed the undesirable direction for marketable fruit yield indicating majority of the hybrids exhibited unfavorable heterotic response and only a few hybrids could be considered for selection.


2021 ◽  
Vol 17 (2) ◽  
pp. 446-454
Author(s):  
A. Rajani ◽  
L. Naram Naidu ◽  
R.V.S.K. Reddy ◽  
D. Ratna Babu ◽  
K. Umakrishna

The present investigation was conducted on heterosis for fruit yield quality attributing characters in okra. Fifteen F1 hybrids were generated by half diallel (excluding reciprocals) mating design. These F1 hybrids along with six parents were evaluated in Randomized Block Design with three replications during late Rabi season of 2018 at three locations HRS, Lam; KVK, V.R.Gudem and KVK, Vonipenta, Andhra Pradesh. Observations were recorded for five randomly selected and tagged plants from each treatment for fruit yield and quality attributing characters viz., number of fruits per plant, fruit length (cm), fruit girth (cm), fruit weight (g), number of seeds per fruit, test weight (g/100), fruit yield per plant (g), fruit yield per hectare (t), fibre content (%), ascorbic acid content (mg/100g) and shelf life (days). The values of F1 hybrids averaged over three replications were used for estimating heterosis and pooled data was obtained. The top five heterotic cross combinations viz., 440-10-1 x HRB-9-2, VRO-6 x HRB-9-2, TCR-1674 x HRB-9-2, VRO-6 x JPM-20-16-39 and VRO-3 x HRB-9-2 were identified as stable with desirable heterosis for fruit yield and other important traits.


2020 ◽  
pp. 37-40
Author(s):  
А.В. Корнев ◽  
Л.М. Соколова ◽  
А.Н. Ховрин ◽  
В.И. Леунов ◽  
М.А. Косенко

Селекцией моркови столовой в России занимаются ФГБНУ ФНЦО, Агрохолдинг «Поиск», ООО «Селекционная станция имени Н.Н. Тимофеева» и другие. Цель работы – подбор исходного материала и создание новых линий-опылителей моркови столовой с одновременной доработкой имеющихся в генетической коллекции ВНИИО-филиала ФГБНУ ФНЦО. Исследования проводили в 2011-2019 годах в условиях Московской области на экспериментальной базе и в селекционном центре ВНИИО-филиала ФГБНУ ФНЦО. Материалом для работы служили сорта и гибриды отечественной и иностранной селекции, селекционный материал, полученный от межлинейных и межсортовых скрещиваний. Селекционную работу проводили методом многократного инбридинга (до I3-4). По мере отработанности селекционного материала на устойчивость к альтернариозу и фузариозу, морфологическую выровненность, переходили на сибсовые скрещивания. Селекционный процесс по созданию новых линий-опылителей осуществлялся в питомнике исходного материала и селекционном питомнике. В результате селекционной работы было получено 14 новых линий-опылителей моркови столовой и доработаны 4 линии, имеющиеся в генетической коллекции ВНИИО-филиала ФГБНУ ФНЦО. Большая часть линий-опылителей (5 шт.) принадлежит сортотипу Курода, четыре - Берликум/Нантская, по три – Берликум, Флакке, две – Шантенэ, одна – Нантская. Общая урожайность линий варьирует от 70,9 до 110,0 т/га. Наибольшую урожайность формировали линии 93-2, 98, 56-2. Значительные пределы содержания β-каротина (11,1 – 16,8 мг%) связаны с происхождением линий. Отмечено, что инбредные линии, полученные из исходного материала иностранной селекции содержат меньше β-каротина. Шесть линий относятся к позднеспелым, десять – к среднеспелым, две – к раннеспелым. В результате оценки инбредных линий по устойчивости к грибным болезням установлено, что 16 линий принадлежат к слабовосприимчивым на инфекционных и естественном фонах, две линии – средневосприимчивые: 805 – на инфекционном фоне Alternaria, REW – по двум инфекционным фонам. На естественном фоне все линии относятся к слабовосприимчивым. The selection of carrots in Russia is carried out by FSBSI FSVC, Agroholding «Poisk», LLC «Breeding station named after N.N. Timofeeva» and others. The purpose of the work is to select the initial material and create new pollinating lines for carrots with the simultaneous refinement of those in the genetic collection of the ARRIVG- a branch of the FSBSI FSVC. The studies were carried out in 2011-2019 in the conditions of the Moscow region on the experimental base and in the selection center of the ARRIVG - a branch of the FSBSI FSVC. The material for the work was varieties and hybrids of domestic and foreign selection, breeding material obtained from interline and intervarietal crosses. The breeding work was carried out by the method of multiple inbreeding (up to I3-4). As the breeding material worked out for resistance to Alternariaand Fusarium, morphological uniformity, they switched to sib crosses. The breeding process for the creation of new pollinator lines was carried out in the nursery of the source material and the breeding nursery. As a result of breeding work, 14 new lines-pollinators of carrots were obtained and 4 lines, which are available in the genetic collection of the ARRIVG- a branch of the FSBSI FSVC, were modified. Most of the pollinator lines (5 pcs.) belong to the Kuroda variety type, four - Berlikum/Nantes, three each - Berlikum, Flakke, two - Chantenay, one - Nantes. The total yield of the lines varies from 70.9 to 110.0 t/ha. The highest yield was formed by lines 93-2, 98, 56-2. Significant limits of β-carotene content (11.1 - 16.8 mg%) are associated with the origin of the lines. It was noted that inbred lines obtained from the source material of foreign selection contain less β-carotene. Six lines are late maturing, ten are mid-maturing, two are early maturing. As a result of the assessment of inbred lines for resistance to fungal diseases, it was found that 16 lines belong to weakly susceptible on infectious and natural backgrounds, two lines are moderately susceptible: 805 - according to the infectious background Alternaria, REW - according to two infectious backgrounds. On a natural background, all lines are weakly receptive.


1964 ◽  
Vol 63 (2) ◽  
pp. 235-243 ◽  
Author(s):  
D. A. Bond ◽  
J. L. Fyfe ◽  
Gillian Toynbee-Clarke

1. F1 hybrids between inbred lines of winter beans were obtained by roguing male-fertile plants from segregating progenies of a genetic male-sterile line which had been interplanted with a pollinator.2. In a series of trials the mean yields of many hybrids were significantly higher than those of open-pollinated varieties. Over the 4 years, 1959–62, with trials at three centres in each year, the mean advantages of all hybrids over the two control varieties was 6·4 cwt./acre over Gartons S.Q., 4·7 cwt./acre over Throws M.S.


Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1943
Author(s):  
Cristina Patanè ◽  
Sarah Siah ◽  
Alessandra Pellegrino ◽  
Salvatore L. Cosentino ◽  
Laura Siracusa

The Mediterranean long shelf-life tomato (or long storage tomato) is a plant traditionally cultivated under no irrigation in the Southern regions of Italy, whose fruits have typical high sensory and nutritional quality. However, yield levels are kept low under current cultivation conditions. In this study, the effects of repeated cycles of drying and rehydration on crop productivity and nutritional quality of fruits in terms of polyphenols and carotenoids content were assessed in long shelf-life tomatoes cultivated in a typical semi-arid area of Southern Italy. The three local Sicilian landraces ‘Custonaci’, ‘Salina’ and ‘Vulcano’, and the commercial tomato hybrid ‘Faino’ (control) were submitted to three irrigation treatments: DRY (no irrigation); IRR (long-season full irrigation); REW (drought/rewatering cycles). Total 450, 4710, and 1849 m3 ha−1, were distributed in DRY, IRR, and REW, respectively. At harvest, fruit yield, polyphenols (as total, flavonoids, and hydroxycinnamoyl quinic acids-HCQA), and carotenoids contents (lycopene and β-carotene) were measured. All cultivars benefitted from very limited irrigation in REW, raising their productivity (up to +147% in ‘Vulcano’) with respect to that of plants overstressed in DRY. Irrigation water use efficiency in REW was higher than that in IRR. Water shortage in REW led to a polyphenols content of fruits that was slightly lower (171.1 μg g−1) than that in DRY, but higher than that in IRR (116.8 μg g−1). All local landraces had greater contents (>160 μg g−1) than control (113.0 μg g−1). Under REW and DRY, the two landraces ‘Salina’ and ‘Vulcano’ produced fruits with the same polyphenols and flavonoids contents. Overall, the two water stressed treatments (DRY and REW) did not differ for HCQA content (>66 μg g−1), which was significantly higher than that in the irrigated plots (<50.55 μg g−1). Lycopene content in DRY and REW exceeded 100 μg g−1; among local types, ‘Vulcano’ exhibited the highest content (106.7 μg g−1). The β-carotene content did not change in ‘Custonaci’ and ‘Salina’, whereas in ‘Vulcano’ it was greater in DRY and REW. Only in ‘Faino’ water limitation in REW exerted a clear promoting effect of on β-carotene content of fruits. The results of the present study reveal how rewatering after long-lasting drought periods is beneficial to long shelf-life tomatoes in terms of productivity. Appropriate drought cycles in REW may also help the fruits to retain high nutritional levels.


1998 ◽  
Vol 21 (1) ◽  
pp. 99-103
Author(s):  
João Alencar de Sousa ◽  
Wilson Roberto Maluf

This study was carried out to obtain estimates of heterosis in crosses between seven eggplant cultivars (Embu = E; Santa Genebra = SG; Viserba = V; Aubergine de Barbentane = AB; Florida Market 10 = FM; Black Beauty = BB, and Melitino = M) and two breeding lines (B-14-07 = B1 and B-31-06 = B2). The F1 hybrids used were: E x FM; E x BB; E x M; E x B1; E x B2; SG x FM; SG x BB; SG x M; SG x B1; SG x B2; V x FM; V x B1; V x B2; AB x FM; AB x M; AB x B1; AB x B2 and M x FM. Cultivars, lines and hybrids were evaluated at the ESAL experimental field in Lavras, MG, from February to October 1992. A randomized complete block design with three replications was used. Significant heterosis relative to the parental means was detected for all traits studied. Their values ranged from +41.23% to +113.31% for total fruit yield, from -11.45% to +26.17% for average fruit weight, and from +27.98% to +141.81% for early production. Heterosis relative to the superior parent ranged from +13.89% to +92.51% for total fruit yield. Hybrid pairs: SG x FM and AB x B1, V x FM and AB x FM, E x M and AB x B1 were the most heterotic relative to the parental mean for total fruit production, mean fruit weight and early production, respectively. The hybrids displaying highest heterosis relative to the superior parent for total yield were AB x B1 and SG x FM.


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