scholarly journals Critical Analysis of Non-Thermal Plasma-Driven Modulation of Immune Cells from Clinical Perspective

2020 ◽  
Vol 21 (17) ◽  
pp. 6226 ◽  
Author(s):  
Barbora Smolková ◽  
Adam Frtús ◽  
Mariia Uzhytchak ◽  
Mariia Lunova ◽  
Šárka Kubinová ◽  
...  
Keyword(s):  
Immune Cells ◽  
Thermal Plasma ◽  
Critical Analysis ◽  
Cell Function ◽  
Immune Cell ◽  
Real Life ◽  
Research Strategy ◽  
Cellular Mechanisms ◽  
Non Thermal Plasma

The emerged field of non-thermal plasma (NTP) shows great potential in the alteration of cell redox status, which can be utilized as a promising therapeutic implication. In recent years, the NTP field considerably progresses in the modulation of immune cell function leading to promising in vivo results. In fact, understanding the underlying cellular mechanisms triggered by NTP remains incomplete. In order to boost the field closer to real-life clinical applications, there is a need for a critical overview of the current state-of-the-art. In this review, we conduct a critical analysis of the NTP-triggered modulation of immune cells. Importantly, we analyze pitfalls in the field and identify persisting challenges. We show that the identification of misconceptions opens a door to the development of a research strategy to overcome these limitations. Finally, we propose the idea that solving problems highlighted in this review will accelerate the clinical translation of NTP-based treatments.

scholarly journals Multi-Compartment 3D-Cultured Organ-on-a-Chip: Towards a Biomimetic Lymph Node for Drug Development

Pharmaceutics ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 464 ◽  
Author(s):  
Aya Shanti ◽  
Bisan Samara ◽  
Amal Abdullah ◽  
Nicholas Hallfors ◽  
Dino Accoto ◽  
...  
Keyword(s):  
Lymph Node ◽  
Drug Development ◽  
Immune Cells ◽  
Cell Function ◽  
Immune Cell ◽  
Building Blocks ◽  
Cell Types ◽  
Matrix Composition ◽  
Encapsulated Cells

The interaction of immune cells with drugs and/or with other cell types should be mechanistically investigated in order to reduce attrition of new drug development. However, they are currently only limited technologies that address this need. In our work, we developed initial but significant building blocks that enable such immune-drug studies. We developed a novel microfluidic platform replicating the Lymph Node (LN) microenvironment called LN-on-a-chip, starting from design all the way to microfabrication, characterization and validation in terms of architectural features, fluidics, cytocompatibility, and usability. To prove the biomimetics of this microenvironment, we inserted different immune cell types in a microfluidic device, which showed an in-vivo-like spatial distribution. We demonstrated that the developed LN-on-a-chip incorporates key features of the native human LN, namely, (i) similarity in extracellular matrix composition, morphology, porosity, stiffness, and permeability, (ii) compartmentalization of immune cells within distinct structural domains, (iii) replication of the lymphatic fluid flow pattern, (iv) viability of encapsulated cells in collagen over the typical timeframe of immunotoxicity experiments, and (v) interaction among different cell types across chamber boundaries. Further studies with this platform may assess the immune cell function as a step forward to disclose the effects of pharmaceutics to downstream immunology in more physiologically relevant microenvironments.

scholarly journals Immune Cell Modulation of the Extracellular Matrix Contributes to the Pathogenesis of Pancreatic Cancer

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 901
Author(s):  
Ramiz S. Ahmad ◽  
Timothy D. Eubank ◽  
Slawomir Lukomski ◽  
Brian A. Boone
Keyword(s):  
Pancreatic Cancer ◽  
Extracellular Matrix ◽  
Immune Cells ◽  
Immune Cell ◽  
Treatment Strategies ◽  
Stellate Cells ◽  
Pancreatic Stellate Cells ◽  
Ductal Adenocarcinoma ◽  
Cellular Mechanisms ◽  
Novel Treatment Strategies

Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal malignancy with a five-year survival rate of only 9%. PDAC is characterized by a dense, fibrotic stroma composed of extracellular matrix (ECM) proteins. This desmoplastic stroma is a hallmark of PDAC, representing a significant physical barrier that is immunosuppressive and obstructs penetration of cytotoxic chemotherapy agents into the tumor microenvironment (TME). Additionally, dense ECM promotes hypoxia, making tumor cells refractive to radiation therapy and alters their metabolism, thereby supporting proliferation and survival. In this review, we outline the significant contribution of fibrosis to the pathogenesis of pancreatic cancer, with a focus on the cross talk between immune cells and pancreatic stellate cells that contribute to ECM deposition. We emphasize the cellular mechanisms by which neutrophils and macrophages, specifically, modulate the ECM in favor of PDAC-progression. Furthermore, we investigate how activated stellate cells and ECM influence immune cells and promote immunosuppression in PDAC. Finally, we summarize therapeutic strategies that target the stroma and hinder immune cell promotion of fibrogenesis, which have unfortunately led to mixed results. An enhanced understanding of the complex interactions between the pancreatic tumor ECM and immune cells may uncover novel treatment strategies that are desperately needed for this devastating disease.

scholarly journals Non-thermal plasma-treated solution demonstrates antitumor activity against pancreatic cancer cells in vitro and in vivo

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Kim Rouven Liedtke ◽  
Sander Bekeschus ◽  
André Kaeding ◽  
Christine Hackbarth ◽  
Jens-Peter Kuehn ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Antitumor Activity ◽  
Cancer Cells ◽  
Thermal Plasma ◽  
Pancreatic Cancer Cells ◽  
Non Thermal Plasma

scholarly journals Erratum: Bisphosphonate-induced differential modulation of immune cell function in gingiva and bone marrow in vivo: Role in osteoclast-mediated NK cell activation

Oncotarget ◽  
2015 ◽  
Vol 6 (38) ◽  
pp. 41398-41398 ◽  
Author(s):  
Han-Ching Tseng ◽  
Keiichi Kanayama ◽  
Kawaljit Kaur ◽  
So-Hyun Park ◽  
Sil Park ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Activation ◽  
Cell Function ◽  
Immune Cell ◽  
Nk Cell ◽  
Differential Modulation ◽  
Immune Cell Function

scholarly journals Updates on Immunotherapy and Immune Landscape in Renal Clear Cell Carcinoma

Cancers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 5856
Author(s):  
Myung-Chul Kim ◽  
Zeng Jin ◽  
Ryan Kolb ◽  
Nicholas Borcherding ◽  
Jonathan Alexander Chatzkel ◽  
...  
Keyword(s):  
T Cell ◽  
Immune Cells ◽  
Immune Cell ◽  
Single Cell Analysis ◽  
Clear Cell ◽  
Cd8 T Cell ◽  
Cell Infiltration ◽  
Cellular Mechanisms ◽  
T Cell Infiltration ◽  
Immunological Mechanisms

Several clinicopathological features of clear cell renal cell carcinomas (ccRCC) contribute to make an “atypical” cancer, including resistance to chemotherapy, sensitivity to anti-angiogenesis therapy and ICIs despite a low mutational burden, and CD8+ T cell infiltration being the predictor for poor prognosis–normally CD8+ T cell infiltration is a good prognostic factor in cancer patients. These “atypical” features have brought researchers to investigate the molecular and immunological mechanisms that lead to the increased T cell infiltrates despite relatively low molecular burdens, as well as to decipher the immune landscape that leads to better response to ICIs. In the present study, we summarize the past and ongoing pivotal clinical trials of immunotherapies for ccRCC, emphasizing the potential molecular and cellular mechanisms that lead to the success or failure of ICI therapy. Single-cell analysis of ccRCC has provided a more thorough and detailed understanding of the tumor immune microenvironment and has facilitated the discovery of molecular biomarkers from the tumor-infiltrating immune cells. We herein will focus on the discussion of some major immune cells, including T cells and tumor-associated macrophages (TAM) in ccRCC. We will further provide some perspectives of using molecular and cellular biomarkers derived from these immune cell types to potentially improve the response rate to ICIs in ccRCC patients.

scholarly journals Drosophila immune cells extravasate from vessels to wounds using Tre1 GPCR and Rho signaling

2018 ◽  
Vol 217 (9) ◽  
pp. 3045-3056 ◽  
Author(s):  
Leila Thuma ◽  
Deborah Carter ◽  
Helen Weavers ◽  
Paul Martin
Keyword(s):  
Immune Cells ◽  
Immune Cell ◽  
Innate Immune ◽  
Murine Models ◽  
Pupal Development ◽  
Epithelial Migration ◽  
Drosophila Model ◽  
Rate Limiting ◽  
Insight Into

Inflammation is pivotal to fight infection, clear debris, and orchestrate repair of injured tissues. Although Drosophila melanogaster have proven invaluable for studying extravascular recruitment of innate immune cells (hemocytes) to wounds, they have been somewhat neglected as viable models to investigate a key rate-limiting component of inflammation—that of immune cell extravasation across vessel walls—due to their open circulation. We have now identified a period during pupal development when wing hearts pulse hemolymph, including circulating hemocytes, through developing wing veins. Wounding near these vessels triggers local immune cell extravasation, enabling live imaging and correlative light-electron microscopy of these events in vivo. We show that RNAi knockdown of immune cell integrin blocks diapedesis, just as in vertebrates, and we uncover a novel role for Rho-like signaling through the GPCR Tre1, a gene previously implicated in the trans-epithelial migration of germ cells. We believe this new Drosophila model complements current murine models and provides new mechanistic insight into immune cell extravasation.

scholarly journals In vivo study of non-invasive effects of non-thermal plasma in pressure ulcer treatment

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Maedeh Chatraie ◽  
Giti Torkaman ◽  
Mohammadreza Khani ◽  
Hossein Salehi ◽  
Babak Shokri
Keyword(s):  
Pressure Ulcer ◽  
Thermal Plasma ◽  
In Vivo Study ◽  
Non Invasive ◽  
Non Thermal Plasma ◽  
Ulcer Treatment

scholarly journals Administration of cardiac mesenchymal cells modulates innate immunity in the acute phase of myocardial infarction in mice

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yi Kang ◽  
Marjan Nasr ◽  
Yiru Guo ◽  
Shizuka Uchida ◽  
Tyler Weirick ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Innate Immunity ◽  
Mechanism Of Action ◽  
Cell Activation ◽  
Cell Function ◽  
Immune Cell ◽  
Mesenchymal Cell ◽  
Cell Types

Abstract Although cardiac mesenchymal cell (CMC) therapy mitigates post-infarct cardiac dysfunction, the underlying mechanisms remain unidentified. It is acknowledged that donor cells are neither appreciably retained nor meaningfully contribute to tissue regeneration—suggesting a paracrine-mediated mechanism of action. As the immune system is inextricably linked to wound healing/remodeling in the ischemically injured heart, the reparative actions of CMCs may be attributed to their immunoregulatory properties. The current study evaluated the consequences of CMC administration on post myocardial infarction (MI) immune responses in vivo and paracrine-mediated immune cell function in vitro. CMC administration preferentially elicited the recruitment of cell types associated with innate immunity (e.g., monocytes/macrophages and neutrophils). CMC paracrine signaling assays revealed enhancement in innate immune cell chemoattraction, survival, and phagocytosis, and diminished pro-inflammatory immune cell activation; data that identifies and catalogues fundamental immunomodulatory properties of CMCs, which have broad implications regarding the mechanism of action of CMCs in cardiac repair.

scholarly journals 58 Functional nutrition to modulate the immune response

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 66-66
Keyword(s):  
Immune Cells ◽  
Cell Function ◽  
Immune Cell ◽  
Animal Feed ◽  
Consumer Preference ◽  
Companion Animal ◽  
Feed Additives ◽  
Cellular Migration ◽  
Lipid Mediator ◽  
Omega 3

Abstract Growing concerns about antibiotic resistance coupled with recent legislation limiting in-feed antibiotic use in livestock feed has led to a resurgence of interest in plant-based extracts, prebiotics/probiotics, and other nutraceutical compounds with immune-modulating properties. Consumer preference for functional ingredients in their own food as well as feed for their pets or livestock has created a niche for development of feed additives with bioactive properties with the potential to improve health through their ability to regulate inflammation and modulate microbial communities. For example, dietary omega-3 fatty acids have been documented to reduce inflammation through altering the fatty acid composition of immune cells, shifting immune cell function, and reducing inflammatory lipid mediator and cytokine production. Dietary probiotic supplementation allows an intimate interaction between the gut mucosa and the largest concentrated population of the host’s immune cells, the mucosal immune system. Probiotics have been shown to modulate the inflammatory response through interaction with intestinal epithelial cells, M-cells in Peyer’s patches, innate immune cells such as dendritic and other antigen-presenting cells, and increasing barrier function through increased mucus and antibody production. Other feedstuffs such as green tea components (epigallocatechin-3-gallate, EGCG) interact with neutrophils and macrophages to limit cellular migration and inflammatory molecule production to reduce inflammation. A wealth of information detailing mechanistic outcomes exists in mouse literature and human cell lines, while livestock and companion animal literature is an untapped area for future work. An emphasis on research aimed at a more molecular, mechanistic understanding of currently available feed additives will lead to the improvement of existing additives and development of new additives for use in livestock and companion animal feed.

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