scholarly journals Silencing of HvGSK1.1—A GSK3/SHAGGY-Like Kinase–Enhances Barley (Hordeum vulgare L.) Growth in Normal and in Salt Stress Conditions

2020 ◽  
Vol 21 (18) ◽  
pp. 6616
Author(s):  
Yuliya Kloc ◽  
Marta Dmochowska-Boguta ◽  
Andrzej Zielezinski ◽  
Anna Nadolska-Orczyk ◽  
Wojciech M. Karlowski ◽  
...  
Keyword(s):  
Salt Stress ◽  
Glycogen Synthase ◽  
Transcript Level ◽  
Kernel Weight ◽  
Stress Conditions ◽  
Promoter Regions ◽  
Hordeum Vulgare L ◽  
Strong Negative Correlation ◽  
Developmental Processes ◽  
Wide Range

Glycogen synthase kinase 3 (GSK3) is a highly conserved kinase present in all eukaryotes and functions as a key regulator of a wide range of physiological and developmental processes. The kinase, known in land plants as GSK3/SHAGGY-like kinase (GSK), is a key player in the brassinosteroid (BR) signaling pathway. The GSK genes, through the BRs, affect diverse developmental processes and modulate responses to environmental factors. In this work, we describe functional analysis of HvGSK1.1, which is one of the GSK3/SHAGGY-like orthologs in barley. The RNAi-mediated silencing of the target HvGSK1.1 gene was associated with modified expression of its paralogs HvGSK1.2, HvGSK2.1, HvGSK3.1, and HvGSK4.1 in plants grown in normal and in salt stress conditions. Low nucleotide similarity between the silencing fragment and barley GSK genes and the presence of BR-dependent transcription factors’ binding sites in promoter regions of barley and rice GSK genes imply an innate mechanism responsible for co-regulation of the genes. The results of the leaf inclination assay indicated that silencing of HvGSK1.1 and the changes of GSK paralogs enhanced the BR-dependent signaling in the plants. The strongest phenotype of transgenic lines with downregulated HvGSK1.1 and GSK paralogs had greater biomass of the seedlings grown in normal conditions and salt stress as well as elevated kernel weight of plants grown in normal conditions. Both traits showed a strong negative correlation with the transcript level of the target gene and the paralogs. The characteristics of barley lines with silenced expression of HvGSK1.1 are compatible with the expected phenotypes of plants with enhanced BR signaling. The results show that manipulation of the GSK-encoding genes provides data to explore their biological functions and confirm it as a feasible strategy to generate plants with improved agricultural traits.

scholarly journals Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization inIris. lacteavar.chinensisRoots under Cadmium, Lead, and Salt Stress Conditions

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Chun-Sun Gu ◽  
Liang-qin Liu ◽  
Chen Xu ◽  
Yan-hai Zhao ◽  
Xu-dong Zhu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Salt Stress ◽  
Real Time ◽  
Reference Genes ◽  
Elongation Factor ◽  
Stress Conditions ◽  
Translation Initiation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Wide Range

Quantitative real time PCR (RT-qPCR) has emerged as an accurate and sensitive method to measure the gene expression. However, obtaining reliable result depends on the selection of reference genes which normalize differences among samples. In this study, we assessed the expression stability of seven reference genes, namely, ubiquitin-protein ligase UBC9 (UBC), tubulin alpha-5 (TUBLIN), eukaryotic translation initiation factor (EIF-5A), translation elongation factor EF1A (EF1α), translation elongation factor EF1B (EF1b), actin11 (ACTIN), and histone H3 (HIS), inIris. lacteavar.chinensis(I. lacteavar.chinensis) root when the plants were subjected to cadmium (Cd), lead (Pb), and salt stress conditions. All seven reference genes showed a relatively wide range of threshold cycles (Ct) values in different samples. GeNorm and NormFinder algorithms were used to assess the suitable reference genes. The results from the two software units showed thatEIF-5AandUBCwere the most stable reference genes across all of the tested samples, whileTUBLINwas unsuitable as internal controls.I. lacteavar.chinensisis tolerant to Cd, Pb, and salt. Our results will benefit future research on gene expression in response to the three abiotic stresses.

Mapping QTLs for Wheat Seedling Traits in RILs Population of Yanda 1817×Beinong 6 under Normal and Salt-Stress Conditions

2016 ◽  
Vol 42 (12) ◽  
pp. 1764 ◽  
Author(s):  
Sheng-Hui ZHOU ◽  
Qiu-Hong WU ◽  
Jing-Zhong XIE ◽  
Jiao-Jiao CHEN ◽  
Yong-Xing CHEN ◽  
...  
Keyword(s):  
Salt Stress ◽  
Wheat Seedling ◽  
Stress Conditions ◽  
Seedling Traits

Potential for plant growth promotion of Kocuria arsenatis Strain ST19 on tomato under salt stress conditions

2021 ◽  
Vol 138 ◽  
pp. 94-104
Author(s):  
Guendouz Dif ◽  
Hadj Ahmed Belaouni ◽  
Yacine Goudjal ◽  
Amine Yekkour ◽  
Nadjette Djemouai ◽  
...  
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Stress Conditions

scholarly journals Thiourea and hydrogen peroxide priming improved K+ retention and source-sink relationship for mitigating salt stress in rice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Manish Pandey ◽  
Radha Krishna Paladi ◽  
Ashish Kumar Srivastava ◽  
Penna Suprasanna
Keyword(s):  
Hydrogen Peroxide ◽  
Salt Stress ◽  
Rice Variety ◽  
Nacl Stress ◽  
Stress Conditions ◽  
Field Conditions ◽  
Growth And Yield ◽  
Yield Attributes ◽  
Redox Environment ◽  
Source Sink

AbstractPlant bioregulators (PBRs) represent low-cost chemicals for boosting plant defense, especially under stress conditions. In the present study, redox based PBRs such as thiourea (TU; a non-physiological thiol-based ROS scavenger) and hydrogen peroxide (H2O2; a prevalent biological ROS) were assessed for their ability to mitigate NaCl stress in rice variety IR 64. Despite their contrasting redox chemistry, TU or H2O2 supplementation under NaCl [NaCl + TU (NT) or NaCl + H2O2 (NH)] generated a reducing redox environment in planta, which improved the plant growth compared with those of NaCl alone treatment. This was concomitant with better K+ retention and upregulated expression of NaCl defense related genes including HAK21, LEA1, TSPO and EN20 in both NT and NH treated seedlings. Under field conditions, foliar applications of TU and H2O2, at vegetative growth, pre-flowering and grain filling stages, increased growth and yield attributes under both control and NaCl stress conditions. Principal component analysis revealed glutathione reductase dependent reduced ROS accumulation in source (flag leaves) and sucrose synthase mediated sucrose catabolism in sink (developing inflorescence), as the key variables associated with NT and NH mediated effects, respectively. In addition, photosystem-II efficiency, K+ retention and source-sink relationship were also improved in TU and H2O2 treated plants. Taken together, our study highlights that reducing redox environment acts as a central regulator of plant’s tolerance responses to salt stress. In addition, TU and H2O2 are proposed as potential redox-based PBRs for boosting rice productivity under the realistic field conditions.

scholarly journals Developmental perspectives on personality: implications for ecological and evolutionary studies of individual differences

2010 ◽  
Vol 365 (1560) ◽  
pp. 4029-4041 ◽  
Author(s):  
Judy A. Stamps ◽  
Ton G. G. Groothuis
Keyword(s):  
Developmental Plasticity ◽  
Niche Construction ◽  
Prior Experience ◽  
Life Stage ◽  
Animal Personality ◽  
Temporal Consistency ◽  
Developmental Processes ◽  
Wide Range ◽  
Evolutionary Studies ◽  
Research Programmes

Developmental processes can have major impacts on the correlations in behaviour across contexts (contextual generality) and across time (temporal consistency) that are the hallmarks of animal personality. Personality can and does change: at any given age or life stage it is contingent upon a wide range of experiential factors that occurred earlier in life, from prior to conception through adulthood. We show how developmental reaction norms that describe the effects of prior experience on a given behaviour can be used to determine whether the effects of a given experience at a given age will affect contextual generality at a later age, and to illustrate how variation within individuals in developmental plasticity leads to variation in contextual generality across individuals as a function of experience. We also show why niche-picking and niche-construction, behavioural processes which allow individuals to affect their own developmental environment, can affect the contextual generality and the temporal consistency of personality. We conclude by discussing how an appreciation of developmental processes can alert behavioural ecologists studying animal personality to critical, untested assumptions that underlie their own research programmes, and outline situations in which a developmental perspective can improve studies of the functional significance and evolution of animal personality.

scholarly journals MES7 Modulates Seed Germination via Regulating Salicylic Acid Content in Arabidopsis

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 903
Author(s):  
Wenrui Gao ◽  
Yan Liu ◽  
Juan Huang ◽  
Yaqiu Chen ◽  
Chen Chen ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Seed Germination ◽  
Environmental Conditions ◽  
Acid Content ◽  
Hydrolytic Enzyme ◽  
Stress Conditions ◽  
Transitional Period ◽  
Highly Sensitive

Seed germination is an important phase transitional period of angiosperm plants during which seeds are highly sensitive to different environmental conditions. Although seed germination is under the regulation of salicylic acid (SA) and other hormones, the molecular mechanism underlying these regulations remains mysterious. In this study, we determined the expression of SA methyl esterase (MES) family genes during seed germination. We found that MES7 expression decreases significantly in imbibed seeds, and the dysfunction of MES7 decreases SA content. Furthermore, MES7 reduces and promotes seed germination under normal and salt stress conditions, respectively. The application of SA restores the seed germination deficiencies of mes7 mutants under different conditions. Taking together, our observations uncover a MeSA hydrolytic enzyme, MES7, regulates seed germination via altering SA titer under normal and abiotic stress conditions.

Post-anthesis sink size in a high-yielding dwarf wheat: yield response to grain number

1977 ◽  
Vol 28 (2) ◽  
pp. 165 ◽  
Author(s):  
RA Fischer ◽  
I Aguilar ◽  
DR Laing
Keyword(s):  
Grain Yield ◽  
Wheat Yield ◽  
Kernel Weight ◽  
Yield Response ◽  
High Fertility ◽  
Grain Number ◽  
Wide Range ◽  
Carbon Dioxide Fertilization ◽  
Sink Size ◽  
The One

Experiments to study the effect of grain number per sq metre on kernel weight and grain yield in a high-yielding dwarf spring wheat (Triticum aestivum cv. Yecora 70) were conducted in three seasons (1971–1973) under high-fertility irrigated conditions in north-western Mexico. Crop thinning, shading and carbon dioxide fertilization (reported elsewhere), and crowding treatments, all carried out at or before anthesis, led to a wide range in grain numbers (4000 to 34,000/m2). Results indicated the response of grain yield to changing sink size (grains per sq metre), with the post-anthesis environment identical for all crops each year, and with all but the thinner crops intercepting most of the post-anthesis solar radiation. Kernel weight fell linearly with increase in grain number over the whole range of grain numbers studied, but the rate of fall varied with the season. Grain yield, however, increased, reaching a maximum at grain numbers well above those of crops grown with optimal agronomic management but without manipulation. It was concluded that the grain yield in normal crops was limited by both sink and post-anthesis source. There was some doubt, however, as to the interpretation of results from crowded crops, because of likely artificial increases in crop respiration on the one hand, and on the other, in labile carbohydrate reserves in the crops at anthesis. Also deterioration in grain plumpness (hectolitre weight) complicates the simple inference that further gains in yield can come from increased grain numbers alone.

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