scholarly journals Effects of Substrate-Coating Materials on the Wound-Healing Process

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2775 ◽  
Author(s):  
Jin-Young Lin ◽  
Kai-Yin Lo ◽  
Yung-Shin Sun
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Petri Dish ◽  
Wound Healing Process ◽  
Collective Cell Migration ◽  
Dependent Manner ◽  
Wound Healing Assay ◽  
Coating Materials ◽  
Coated Surfaces ◽  
Physical And Chemical

The wound-healing assay is commonly and widely used for investigating collective cell migration under various physical and chemical stimuli. Substrate-coating materials are shown to affect the wound-healing process in a cell-type dependent manner. However, experiment-to-experiment variations make it difficult to compare results from different assays. In this paper, a modified barrier wound-healing assay was reported for studying the wound-healing process on different substrates in one single petri dish. In short, half of a dish was covered with the tape, and coating materials, poly-l-lysine and gelatin, were applied to the surface. After peeling off the tape, half of the surface was coated with the desired material. Then a customized barrier was placed inside the dish to create the wound. The results indicated that surface coating did not affect cell proliferation/viability, and the wound-healing rate increased in coated surfaces compared to uncoated ones. The present study provides a platform for further understanding the mechanisms of substrate coating-dependent wound-healing processes.

scholarly journals A microfluidics-based wound-healing assay for studying the effects of shear stresses, wound widths, and chemicals on the wound-healing process

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jin-Young Lin ◽  
Kai-Yin Lo ◽  
Yung-Shin Sun
Keyword(s):  
Wound Healing ◽  
Shear Stress ◽  
Healing Process ◽  
Wound Healing Process ◽  
Shear Stresses ◽  
Collective Cell Migration ◽  
Wound Healing Assay ◽  
Normal Medium ◽  
Accelerate Wound Healing ◽  
Percent Area

AbstractCollective cell migration plays important roles in various physiological processes. To investigate this collective cellular movement, various wound-healing assays have been developed. In these assays, a “wound” is created mechanically, chemically, optically, or electrically out of a cellular monolayer. Most of these assays are subject to drawbacks of run-to-run variations in wound size/shape and damages to cells/substrate. Moreover, in all these assays, cells are cultured in open, static (non-circulating) environments. In this study, we reported a microfluidics-based wound-healing assay by using the trypsin flow-focusing technique. Fibroblasts were first cultured inside this chip to a cellular monolayer. Then three parallel fluidic flows (containing normal medium and trypsin solution) were introduced into the channels, and cells exposed to protease trypsin were enzymatically detached from the surface. Wounds of three different widths were generated, and subsequent wound-healing processes were observed. This assay is capable of creating three or more wounds of different widths for investigating the effects of various physical and chemical stimuli on wound-healing speeds. The effects of shear stresses, wound widths, and β-lapachone (a wound healing-promoting chemical) on wound-healing speeds were studied. It was found that the wound-healing speed (total area healed per unit time) increased with increasing shear stress and wound width, but under a shear stress of 0.174 mPa the linear healing speed (percent area healed per unit time) was independent of the wound width. Also, the addition of β-lapachone up to 0.5 μM did not accelerate wound healing. This microfluidics-based assay can definitely help in understanding the mechanisms of the wound-healing process and developing new wound-healing therapies.

scholarly journals A Wound-Healing Assay Based on Ultraviolet Light Ablation

2016 ◽  
Vol 22 (1) ◽  
pp. 36-43 ◽  
Author(s):  
Shang-Ying Wu ◽  
Yung-Shin Sun ◽  
Kuan-Chen Cheng ◽  
Kai-Yin Lo
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Healing Process ◽  
Cell Monolayer ◽  
Wound Healing Process ◽  
Collective Cell Migration ◽  
Wound Healing Assay ◽  
Physiological Processes ◽  
Free Region ◽  
A Cell

Collective cell migration plays important roles in many physiological processes such as embryonic development, tissue repair, and angiogenesis. A “wound” occurs when epithelial cells are lost and/or damaged due to some external factors, and collective cell migration takes place in the following wound-healing process. To study this cellular behavior, various kinds of wound-healing assays are developed. In these assays, a “wound,” or a “cell-free region,” is created in a cell monolayer mechanically, chemically, optically, or electrically. These assays are useful tools in studying the effects of certain physical or chemical stimuli on the wound-healing process. Most of these methods have disadvantages such as creating wounds of different sizes or shapes, yielding batch-to-batch variation, and damaging the coating of the cell culture surface. In this study, we used ultraviolet (UV) lights to selectively kill cells and create a wound out of a cell monolayer. A comparison between the current assay and the traditional scratch assay was made, indicating that these two methods resulted in similar wound-healing rates. The advantages of this UV-created wound-healing assay include fast and easy procedure, high throughput, and no direct contact to cells.

Glucocorticoid-induced contractility and F-actin content of human lung fibroblasts in three-dimensional culture

2000 ◽  
Vol 278 (1) ◽  
pp. L13-L18 ◽  
Author(s):  
Hiroyuki Miki ◽  
Tadashi Mio ◽  
Sonoko Nagai ◽  
Yuma Hoshino ◽  
Takeo Tsutsumi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Lung ◽  
Healing Process ◽  
Lung Fibroblast ◽  
Lung Fibroblasts ◽  
Wound Healing Process ◽  
Architectural Distortion ◽  
Type I ◽  
Dependent Manner ◽  
Human Lung Fibroblast

Fibroblast contractility plays a useful role in the wound healing process but contributes to architectural distortion in the lungs. Glucocorticoids (GCs) have been reported to reduce dermal fibroblast contractility, which may result in delaying wound healing, but the effects on lung fibroblasts are unknown. In this study, we examined how human lung fibroblast contractility is altered in the presence of GCs. Lung fibroblast cell lines ( n = 5) were established from normal parts of surgically resected lung tissue. The effects of GCs on contractility were investigated with a type I collagen gel contraction assay. Filamentous actin (F-actin) content was detected by confocal microscopy and measured with a fluorescent phalloidin binding assay. GCs augmented fibroblast contraction in a concentration-dependent manner, with an approximate EC50 of 1.8 × 10−8 M, whereas other steroid derivatives had no effects. GC contractility needed de novo protein synthesis. The GC-induced increase in contractility was found to be consistent with an increase in F-actin content. In conclusion, lung fibroblast contractility was enhanced with GCs through an upregulation of lung fibroblast F-actin.

scholarly journals Efektivitas Sediaan Salep Limbah Kulit Singkong Sebagai Penyembuh Luka

2019 ◽  
Vol 5 (2) ◽  
pp. 133
Author(s):  
Widia Nila Risnadewi ◽  
Nadya Arianita Turisia ◽  
Alsifah Nurhidayati ◽  
Candra Dwipayana Hamdin
Keyword(s):  
Wound Healing ◽  
Wound Care ◽  
Healing Process ◽  
Ethanol Extract ◽  
Good Effect ◽  
Wound Healing Process ◽  
Tissue Integrity ◽  
Herbal Plants ◽  
Physical And Chemical

The skin is an important organ that functions to receive stimuli such as touch, pain and other influences from the outside. Some problems that occur on the skin can becaused because the skin is very vulnerable to trauma and injury. Wound is a condition of the breakdown of tissue integrity caused by violence both mechanical, physical and chemical in nature. In general, wound care in the community is done with a dressing accompanied by compresses betadine and normal saline because these materials are easily available. However, long-term use of the dressing can cause slow wound healing and various infections can appear. This causes people to look for other alternative treatments, one of them with herbal plants, namely cassava plants have the potential to be used as a wound healing and is able to inhibit bacteria because they are suspected to contain phenolic compounds. This study was a true experimental study, the group of test animals was divided into 6 groups, after that the making of wounds and smeared with bacteria were given ointments with 3 concentrations namely 5%, 10%, and 15%. Ointment extract of cassava skin waste gives effect to the wound healing process seen from the length of the wound. The ethanol extract ointment which has a good effect on wound healing is an ointment with a concentration of 10% and 15%.

scholarly journals Polydopamine Antioxidant Hydrogels for Wound Healing Applications

Gels ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 39
Author(s):  
Naphtali A. O’Connor ◽  
Abdulhaq Syed ◽  
Madeline Wong ◽  
Josiah Hicks ◽  
Greisly Nunez ◽  
...  
Keyword(s):  
Wound Healing ◽  
Therapeutic Strategy ◽  
Chronic Wounds ◽  
Ex Vivo ◽  
Healing Process ◽  
Antioxidant Properties ◽  
Feed Ratio ◽  
Wound Healing Process ◽  
Wound Healing Assay ◽  
Hydrogel Synthesis

Antioxidants are known to improve the wound healing process and are researched as a therapeutic strategy to treat chronic wounds. Dopamine is a known neurotransmitter with antioxidant properties that can be polymerized to form polydopamine (PDA). Herein, polydopamine is demonstrated as an antioxidant biomaterial. In prior work, we developed methodology to prepare hydrogels by crosslinking polysaccharides with polyamines via epichlorohydrin and NaOH. Using this previously developed methodology, dextran hydrogels crosslinked with polydopamine were prepared. Darkening of the gels indicated the increasing incorporation of polydopamine within the hydrogels. In addition to basic pH, polydopamine can be formed by reaction with polyethylene imine (PEI), which results in PEI-PDA copolymer. Dextran was similarly crosslinked with the PEI-PDA copolymer and resulted in sturdier, darker gels, which had more polydopamine incorporated. Hydrogel morphology and strength were dependent on the feed ratios of dopamine. Antioxidant activity of polydopamine containing hydrogel was confirmed and shown to be dependent on the amount of dopamine used in hydrogel synthesis. Hydrogels with 0.5 dopamine to dextran feed ratio scavenged 78.8% of radicals in a 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) antioxidant assay while gels with no dopamine scavenged only 1.4% of radicals. An ex vivo wound healing assay showed considerable cell migration with the PEI-PDA containing hydrogel.

scholarly journals Correction: Lin. et al. Effects of Substrate-Coating Materials on the Wound-Healing Process. Materials 2019, 12, 2775

Materials ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 248
Author(s):  
Jin-Young Lin ◽  
Kai-Yin Lo ◽  
Yung-Shin Sun
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Wound Healing Process ◽  
Coating Materials

The authors wish to make the following correction to this paper [...]

scholarly journals Von Hippel-Lindau (VHL) Protein Antagonist VH298 Improves Wound Healing in Streptozotocin-Induced Hyperglycaemic Rats by Activating Hypoxia-Inducible Factor- (HIF-) 1 Signalling

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Shuo Qiu ◽  
Yachao Jia ◽  
Yunchu Sun ◽  
Pei Han ◽  
Jia Xu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Rat Model ◽  
Healing Process ◽  
Wound Healing Process ◽  
Type I ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Vascular Endothelial ◽  
Vhl Protein

Aims. The purpose of the present research is to investigate the effects of the VHL protein antagonist, VH298, on functional activities of fibroblasts and vascular endothelial cells and the effects on the wound healing process in a streptozotocin-induced hyperglycaemic rat model. Methods. HIF-1α and hydroxy-HIF-1α protein levels in VH298-treated rat fibroblasts (rFb) were measured by immunoblotting, rFb proliferation was detected by the CCK-8 assay, and mRNA levels of related genes were measured by quantitative RT-PCR. In vitro wound healing was simulated by the scratch test; angiogenesis was measured by the human umbilical vein endothelial cell (hUVEC) tube formation assay. VH298 or PBS was locally injected into wounds in rat models with streptozotocin- (STZ-) induced hyperglycaemia, the wound tissues were harvested, and haematoxylin-eosin (HE) and Masson trichrome staining and immunohistochemical processes were conducted. Results. HIF-1α and hydroxy-HIF-1α levels increased in VH298-treated rFb, in a time- and dose-dependent manner. Thirty micromolar VH298 could significantly increase cell proliferation, angiogenesis, and gene expression of type I collagen-α1 (Col1-α1), vascular endothelial growth factor A (VEGF-A), and insulin-like growth factor 1 (IGF-1). The VH298-treated wound had a better healing pattern, activation of HIF-1 signalling, and vascularization. Conclusions. Taken together, VH298 activated the HIF-1 signalling pathway by stabilizing both HIF-1α and hydroxy-HIF-1α. VH298 enhanced rFb functions, promoted hUVEC angiogenesis, and accelerated wound healing in the rat model mimicking diabetes mellitus.

scholarly journals Immuno-modulatory effect of local rhEGF treatment during tissue repair in diabetic ulcers

2018 ◽  
Vol 7 (4) ◽  
pp. 584-594 ◽  
Author(s):  
Natalio García-Honduvilla ◽  
Alberto Cifuentes ◽  
Miguel A Ortega ◽  
Marta Pastor ◽  
Garazi Gainza ◽  
...  
Keyword(s):  
Wound Healing ◽  
T Lymphocytes ◽  
Healing Process ◽  
Local Treatment ◽  
Diabetic Rats ◽  
Wound Healing Process ◽  
Dependent Manner ◽  
Best Response ◽  
Complex Process ◽  
Healing Tissue

Wound healing is a complex process that can be severely impaired due to pathological situations such as diabetes mellitus. Diabetic foot ulcers are a common complication of this pathology and are characterized by an excessive inflammatory response. In this work, the effects of local treatment with recombinant human epidermal growth factor (rhEGF) were studied using a full-thickness wound healing model in streptozotocin-induced diabetic rats. Wound healing process was assessed with different concentrations of rhEGF (0.1, 0.5, 2.0 and 8.0 µg/mL), placebo and both diabetic and non-diabetic controls (n = 53). The macroscopic healing observed in treated diabetic rats was affected by rhEGF concentration. Histologically, we also observed an improvement in the epithelialization, granulation tissue formation and maturation in treated groups, finding again the best response at doses of 0.5 and 2.0 µg/mL. Afterwards, the tissue immune response over time was assessed in diabetic rats using the most effective concentrations of rhEGF (0.5 and 2.0 µg/mL), compared to controls. The presence of macrophages, CD4+ T lymphocytes and CD8+ T lymphocytes, in the reparative tissue was quantified, and cytokine expression was measured by quantitative real-time PCR. rhEGF treatment caused a reduction in the number of infiltrating macrophages in the healing tissue of diabetic, as well as diminished activation of these leukocytes. These findings show that local administration of rhEGF improves the healing process of excisional wounds and the quality of the neoformed tissue in a dose-dependent manner. Besides, this treatment reduces the local inflammation associated with diabetic healing, indicating immuno-modulatory properties.

scholarly journals In Vitro Wound Healing Potential of Stem Extract of Alternanthera sessilis

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Katyakyini Muniandy ◽  
Sivapragasam Gothai ◽  
Woan Sean Tan ◽  
S. Suresh Kumar ◽  
Norhaizan Mohd Esa ◽  
...  
Keyword(s):  
Wound Healing ◽  
Dermal Fibroblast ◽  
Healing Process ◽  
Human Dermal Fibroblast ◽  
Wound Healing Process ◽  
Diabetic Patients ◽  
Dependent Manner ◽  
Impaired Wound Healing ◽  
Alternanthera Sessilis ◽  
Stem Extract

Impaired wound healing is one of the serious problems among the diabetic patients. Currently, available treatments are limited due to side effects and cost effectiveness. In line with that, we attempted to use a natural source to study its potential towards the wound healing process. Therefore, Alternanthera sessilis (A. sessilis), an edible and medicinal plant, was chosen as the target sample for the study. During this investigation, the wound closure properties using stem extract of A. sessilis were analyzed. Accordingly, we analyzed the extract on free radical scavenging capacity and the cell migration of two most prominent cell types on the skin, human dermal fibroblast (NHDF), keratinocytes (HaCaT), and diabetic human dermal fibroblast (HDF-D) to mimic the wound healing in diabetic patients. The bioactive compounds were identified using gas chromatography-mass spectrometry (GC-MS). We discovered that the analysis exhibited a remarkable antioxidant, proliferative, and migratory rate in NHDF, HaCaT, and HDF-D in dose-dependent manner, which supports wound healing process, due to the presence of wound healing associated phytocompounds such as Hexadecanoic acid. This study suggested that the stem extract of A. sessilis might be a potential therapeutic agent for skin wound healing, supporting its traditional medicinal uses.

HUBUNGAN MOBILISASI DINI TERHADAP PENYEMBUHAN LUKA POST OPERASI SECTIO CESARIA

2019 ◽  
Vol 2 (1) ◽  
pp. 14-17
Author(s):  
Nurul Aini Siagian ◽  
Syafira Nusaibah ◽  
Andayani Boang Manalu
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Early Mobilization ◽  
Sectio Caesarea ◽  
Wound Healing Process ◽  
P Value ◽  
Cross Sectional ◽  
Care Services ◽  
Healing Wound ◽  
Rapid Healing

Early mobilization includes factors that can affect the process of wound healing after surgery. Immediate mobilization in stages is very useful for the process of healing wounds and preventing infection and venous thrombosis. The purpose of this study was to determine whether there is a relationship between early mobilization and the process of healing wound post operative sectio caesarea at Sinar Husni General Hospital Medan Helvetia. The research design used was analytic survey with cross sectional approach. The sample in this study used the Consecutive Sampling method of data collection using a checklist sheet conducted on a sample of 19 respondents. The results of the study in this study are the majority of respondents who did early mobilization and who experienced rapid wound healing process as many as 4 people (21%) while the minority of respondents who did early mobilization and who experienced slow wound healing process were 1 person (5.3%). The majority of respondents who did not mobilize early and who experienced a slow wound healing process were 11 people (57.9%) and a minority who did not mobilize early and who experienced rapid healing as many as 4 people (21.1%). Statistical test results obtained p value = 0.046 <0.005. The conclusions of the results of this study indicate there is a relationship between early mobilization and the process of healing post operative sectio of caesarea. Suggestions The results of this study can be applied as a reference to improve nursing care services, especially in providing counseling and assistance to patients.

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