scholarly journals Made in Germany: A Quality Indicator Not Only in the Automobile Industry But Also When It Comes to Skin Replacement: How an Automobile Textile Research Institute Developed a New Skin Substitute

Medicina ◽  
2021 ◽  
Vol 57 (2) ◽  
pp. 143
Author(s):  
Herbert Leopold Haller ◽  
Matthias Rapp ◽  
Daniel Popp ◽  
Sebastian Philipp Nischwitz ◽  
Lars Peter Kamolz
Keyword(s):  
Wound Healing ◽  
Automobile Industry ◽  
Chronic Wounds ◽  
Water Vapor Permeability ◽  
Healing Time ◽  
Development Cooperation ◽  
Skin Substitute ◽  
Vapor Permeability ◽  
Clinical Use ◽  
Research Institute

Successful research and development cooperation between a textile research institute, the German Federal Ministry of Education and Research via the Center for Biomaterials and Organ Substitutes, the University of Tübingen, and the Burn Center of Marienhospital, Stuttgart, Germany, led to the development of a fully synthetic resorbable temporary epidermal skin substitute for the treatment of burns, burn-like syndromes, donor areas, and chronic wounds. This article describes the demands of the product and the steps that were taken to meet these requirements. The material choice was based on the degradation and full resorption of polylactides to lactic acid and its salts. The structure and morphology of the physical, biological, and degradation properties were selected to increase the angiogenetic abilities, fibroblasts, and extracellular matrix generation. Water vapor permeability and plasticity were adapted for clinical use. The available scientific literature was screened for the use of this product. A clinical application demonstrated pain relief paired with a reduced workload, fast wound healing with a low infection rate, and good cosmetic results. A better understanding of the product’s degradation process explained the reduction in systemic oxidative stress shown in clinical investigations compared to other dressings, positively affecting wound healing time and reducing the total area requiring skin grafts. Today, the product is in clinical use in 37 countries. This article describes its development, the indications for product growth over time, and the scientific foundation of treatments.

scholarly journals Scaffolds for Wound Healing Applications

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2010 ◽  
Author(s):  
Irina Negut ◽  
Gabriela Dorcioman ◽  
Valentina Grumezescu
Keyword(s):  
Wound Healing ◽  
Wound Care ◽  
Water Vapor Permeability ◽  
Cellular Migration ◽  
Wound Dressings ◽  
Vapor Permeability ◽  
Therapeutic Effects ◽  
Microbial Invasion ◽  
Polymeric Biomaterials ◽  
Hybrid Biomaterials

In order to overcome the shortcomings related to unspecific and partially efficient conventional wound dressings, impressive efforts are oriented in the development and evaluation of new and effective platforms for wound healing applications. In situ formed wound dressings provide several advantages, including proper adaptability for wound bed microstructure and architecture, facile application, patient compliance and enhanced therapeutic effects. Natural or synthetic, composite or hybrid biomaterials represent suitable candidates for accelerated wound healing, by providing proper air and water vapor permeability, structure for macro- and microcirculation, support for cellular migration and proliferation, protection against microbial invasion and external contamination. Besides being the most promising choice for wound care applications, polymeric biomaterials (either from natural or synthetic sources) may exhibit intrinsic wound healing properties. Several nanotechnology-derived biomaterials proved great potential for wound healing applications, including micro- and nanoparticulate systems, fibrous scaffolds, and hydrogels. The present paper comprises the most recent data on modern and performant strategies for effective wound healing.

scholarly journals From Grafts to Human Bioengineered Vascularized Skin Substitutes

2020 ◽  
Vol 21 (21) ◽  
pp. 8197
Author(s):  
Wasima Oualla-Bachiri ◽  
Ana Fernández-González ◽  
María I. Quiñones-Vico ◽  
Salvador Arias-Santiago
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Chronic Wounds ◽  
Thermal Regulation ◽  
Skin Substitute ◽  
Skin Substitutes ◽  
Normal Wound Healing ◽  
Physiological Homeostasis ◽  
Promising Solution ◽  
Native Skin

The skin plays an important role in the maintenance of the human’s body physiological homeostasis. It acts as a coverage that protects against infective microorganism or biomechanical impacts. Skin is also implied in thermal regulation and fluid balance. However, skin can suffer several damages that impede normal wound-healing responses and lead to chronic wounds. Since the use of autografts, allografts, and xenografts present source limitations and intense rejection associated problems, bioengineered artificial skin substitutes (BASS) have emerged as a promising solution to address these problems. Despite this, currently available skin substitutes have many drawbacks, and an ideal skin substitute has not been developed yet. The advances that have been produced on tissue engineering techniques have enabled improving and developing new arising skin substitutes. The aim of this review is to outline these advances, including commercially available skin substitutes, to finally focus on future tissue engineering perspectives leading to the creation of autologous prevascularized skin equivalents with a hypodermal-like layer to achieve an exemplary skin substitute that fulfills all the biological characteristics of native skin and contributes to wound healing.

Comparison of Efficacy of Unna’s boot and Ozone therapy on Chronic Venous Leg Ulcers: a series of case

2020 ◽  
Vol 9 (9) ◽  
pp. e44996967
Author(s):  
Denise Viviane Ferreira Del Castilo ◽  
Marcello Magri Amaral ◽  
Carla Roberta Tim ◽  
Cintia Cristina Santi Martignago ◽  
Daniela Bezerra Macedo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Chronic Wounds ◽  
Healing Time ◽  
Ozone Treatment ◽  
Leg Ulcers ◽  
Ozone Therapy ◽  
Venous Leg Ulcers ◽  
Wound Healing Time ◽  
Tissue Reduction

The aim of this study was to evaluate and compare the effectiveness of Unna’s boot and ozone therapy on chronic venous leg ulcers. Seven patients with chronic venous leg ulcers were taken into the study and were randomly divided into two groups: Unna’s boot treatment group (BU); Ozone treatment group (OZ). The therapies were performed weekly until wound closure. The morphological descriptive results demonstrated that during the treatments, it was possible to observe the presence of semi-planar borders, granulation tissue, reduction of edema, fibrin and exudate and absence of odor. These morphological modifications were more pronounced in the OZ when compared to the BU. Furthermore, both treatments promoted the same wound healing time, never the less the ozone therapy produced a higher percentage of weekly wound reduction compared to Unna's boot. Unna's boot and ozone therapy treatments appeared to positively impact the course of wound healing in chronic wounds, however the ozone therapy may improve the healing of chronic venous leg ulcers in a higher weekly percentage.

scholarly journals Stem Cells for Cutaneous Wound Healing

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Giles T. S. Kirby ◽  
Stuart J. Mills ◽  
Allison J. Cowin ◽  
Louise E. Smith
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Chronic Wounds ◽  
Healing Process ◽  
Clinical Use ◽  
Cutaneous Wound Healing ◽  
Cell Therapies ◽  
Cutaneous Wound ◽  
Matrix Deposition ◽  
Obesity And Diabetes

Optimum healing of a cutaneous wound involves a well-orchestrated cascade of biological and molecular processes involving cell migration, proliferation, extracellular matrix deposition, and remodelling. When the normal biological process fails for any reason, this healing process can stall resulting in chronic wounds. Wounds are a growing clinical burden on healthcare systems and with an aging population as well as increasing incidences of obesity and diabetes, this problem is set to increase. Cell therapies may be the solution. A range of cell based approaches have begun to cross the rift from bench to bedside and the supporting data suggests that the appropriate administration of stem cells can accelerate wound healing. This review examines the main cell types explored for cutaneous wound healing with a focus on clinical use. The literature overwhelmingly suggests that cell therapies can help to heal cutaneous wounds when used appropriately but we are at risk of clinical use outpacing the evidence. There is a need, now more than ever, for standardised methods of cell characterisation and delivery, as well as randomised clinical trials.

Comparison of Autologous Full-Thickness Gingiva and Skin Substitutes for Wound Healing

2008 ◽  
Vol 17 (10-11) ◽  
pp. 1199-1209 ◽  
Author(s):  
Abraham P. Vriens ◽  
Taco Waaijman ◽  
Henk M. Van Den Hoogenband ◽  
Edith M. De Boer ◽  
Rik J. Scheper ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Chronic Wounds ◽  
Clinical Applications ◽  
Skin Substitute ◽  
Full Thickness ◽  
Skin Substitutes ◽  
Differentiation Markers ◽  
Full Thickness Skin ◽  
Thickness Skin

Ideally tissue-engineered products should maintain the characteristics of the original tissue. For example, skin represents orthokeratinized epithelium and oral gingiva represents parakeratinized epithelium. The aim of this study was to develop an autologous full-thickness gingiva substitute suitable for clinical applications and to compare it with our autologous full-thickness skin substitute that is routinely used for healing chronic wounds. Autologous full-thickness skin and gingiva substitutes were constructed under identical culture conditions from 3-mm punch biopsies isolated from the upper leg or gingiva tissue, respectively. Both consisted of reconstructed epithelia on acellular dermis repopulated with fibroblasts. To compare the characteristics of the original and reconstructed tissue, differential morphological observations and expression of differentiation markers (keratins 6, 10, and 17 and stratum corneum precursors involucrin, loricrin, and SKALP) were determined. Skin and gingiva substitutes were transplanted onto therapy-resistant leg ulcers or tooth extraction sites in order to determine their effects on wound healing. The tissue-engineered constructs maintained many of the differential histological and immunohistochemical characteristics of the original tissues from which they were derived. The skin substitute was orthokeratinized, and the gingiva substitute was parakeratinized. Transplantation of skin (n = 19) and gingiva substitutes (n = 3) resulted in accelerated wound healing with no adverse effects. As identical culture systems were used to generate both the skin and gingiva substitutes, the differences observed in tissue (immuno)histology can be attributed to intrinsic properties of the tissues rather than to environmental factors (e.g., air or saliva). This study emphasizes the importance of closely matching donor sites with the area to be transplanted. Our results represent a large step forward in the area of clinical applications in oral tissue engineering, which have until now greatly lagged behind skin tissue engineering.

scholarly journals Development and Characterization of Biointeractive Gelatin Wound Dressing Based on Extract of Punica granatum Linn

Pharmaceutics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1204
Author(s):  
Marismar F. do Nascimento ◽  
Juliana C. Cardoso ◽  
Tarsizio S. Santos ◽  
Lívia A. Tavares ◽  
Tatiana N. Pashirova ◽  
...  
Keyword(s):  
Wound Healing ◽  
Water Vapor Permeability ◽  
Punica Granatum ◽  
Treated Group ◽  
Control Group ◽  
Vapor Permeability ◽  
Histological Changes ◽  
High Antioxidant Activity ◽  
Gel Membranes

Punica granatum Linn (pomegranate) extracts have been proposed for wound healing due to their antimicrobial, antioxidant, and anti-inflammatory properties. In this work, we designed biointeractive membranes that contain standard extracts of P. granatum for the purpose of wound healing. The used standard extract contained 32.24 mg/g of gallic acid and 41.67 mg/g of ellagic acid, and it showed high antioxidant activity (the concentration of the extract that produces 50% scavenging (IC50) 1.715 µg/mL). Compared to the gelatin-based membranes (GEL), membranes containing P. granatum extracts (GELPG) presented a higher maximal tension (p = 0.021) and swelling index (p = 0.033) and lower water vapor permeability (p = 0.003). However, no difference was observed in the elongation and elastic modulus of the two types of membranes (p > 0.05). Our wound-healing assay showed that a GELPG-treated group experienced a significant increase compared to that of the control group in their wound contraction rates on days 3 (p < 0.01), 7 (p < 0.001), and on day 14 (p < 0.001). The GELPG membranes promoted major histological changes in the dynamics of wound healing, such as improvements in the formation of granular tissue, better collagen deposition and arrangement, and earlier development of cutaneous appendages. Our results suggest that a biointeractive gelatin-based membrane containing P. granatum extracts has a promising potential application for dressings that are used to treat wounds.

On skin substitutes for wound healing: Current products, limitations, and future perspectives

TECHNOLOGY ◽  
2021 ◽  
pp. 1-7
Author(s):  
Sarah Susan Kelangi ◽  
Georgios Theocharidis ◽  
Aristidis Veves ◽  
William G. Austen ◽  
Robert Sheridan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Cost Effective ◽  
Cell Types ◽  
Skin Substitute ◽  
Future Research ◽  
Skin Substitutes ◽  
High Quality ◽  
Skin Cell ◽  
Skin Replacement

Cutaneous wound healing is a highly coordinated process involving numerous molecular pathways that regulate the function of specific skin cell types. One of the key decisions in acute and/or chronic wound healing management is to choose the skin substitute that, based on its composition and/or properties, may act as permanent skin replacement or temporary wound cover. The current products however are limited in their action, especially in the context of large chronic wounds or extensive second-/third-degree burns, due to the risk of developing infection, the limited vascularization, the inability to integrate to host tissue, and the lack in the healed area of skin apparatus responsible for temperature control, pigmentation, immune regulation, and nerve supply. In addition, the high cost of skin substitutes precludes their use in small- and moderate-size burns because they are economically impractical and protract the time to definitive wound closure. Thus, the need to develop cost-effective substitutes of high quality is imperative. The purpose of this review is to discuss current available products, their limitations and to provide some perspectives on future research toward generation of cost-effective, high-quality substitutes.

Regenerative properties of recombinant human erythropoetin in wound healing

2021 ◽  
Vol 9-10 (219-220) ◽  
pp. 52-58
Author(s):  
Mariya Dmitriyeva ◽  
◽  
Medet Toleubayev ◽  
Saken Kozhakhmetov ◽  
Nurbek Igissinov ◽  
...  
Keyword(s):  
Wound Healing ◽  
Tissue Regeneration ◽  
Search Strategy ◽  
Chronic Wounds ◽  
Skin Wound ◽  
Clinical Use ◽  
Wound Treatment ◽  
Skin Wound Healing ◽  
Positive Effects ◽  
Single Mechanism

Erythropoietin is the main stimulator of erythropoiesis, but erythropoietin also has non-hematopoietic effects. The recent data show the positive effects of erythropoietin on tissue regeneration. This review highlights the pathophysiological mechanisms of erythropoietin at different stages of tissue regeneration, and possible clinical applications in wound healing. Aim. Study of the world experience of using erythropoietin for the treatment of experimental wounds and the experience of clinical use for the treatment of patients with chronic wounds. Material and methods. The literature was searched in the databases: Pubmed, EMBASE and Google Scholar using the keywords: "Erythropoietin", "EPO", "wounds", "wound treatment", "VEGF", "re-epithelialization", "skin". The search depth was 20 years. When compiling a search strategy, the use of the title of articles, abstracts, as well as conference materials in English, Russian, Kazakh languages was used. Experimental original articles, narrative reviews were included in this review. Results and discussion. To date, there is a sufficient amount of experimental data confirming the effectiveness of EPO on the pathomorphological processes of skin wound healing, but it is still impossible to determine a single mechanism or cellular function that could be responsible for the ability of tissue regeneration mediated by EPO. Conclusion. Research into the ability of erythropoietin to improve tissue regeneration may lead to new insights into this growth hormone for its regular clinical use in patients. Key words: EPO, wound healing, VEGF, reepithelization, skin.

scholarly journals Effect of Chitosan and Aloe Vera Extract Concentrations on the Physicochemical Properties of Chitosan Biofilms

Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1187
Author(s):  
Cristiana M. P. Yoshida ◽  
Murilo S. Pacheco ◽  
Mariana A. de Moraes ◽  
Patrícia S. Lopes ◽  
Patrícia Severino ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Chronic Wounds ◽  
Healing Process ◽  
Aloe Vera ◽  
Water Vapor Permeability ◽  
Vapor Permeability ◽  
Aloe Barbadensis ◽  
Casting Technique ◽  
Color Parameters ◽  
Chitosan Films

Chitosan films have been extensively studied as dressings in formulations for the treatment of chronic wounds. The incorporation of aloe vera (Aloe barbadensis Miller) into chitosan dressings could potentialize the healing process since aloe vera shows several pharmacological activities. This work aimed to evaluate the effect of aloe vera and chitosan concentrations on the physicochemical properties of the developed films. The films were obtained by casting technique and characterized with respect to their color parameters, morphology, barrier and mechanical properties, and thermal analysis. Results showed that the presence of aloe vera modified the films′ color parameters, changed barrier properties, increased fluid handling capacity (FHC), and decreased water-vapor permeability (WVP). The reduced elongation at break resulted in more rigid films. Aloe vera concentration did not significantly change film properties, but the presence of this gel increased the films′ stability at temperatures below 200 °C, showing similar behavior as chitosan films above 400 °C. The results suggest a crosslinking/complexation between chitosan and aloe vera, which combine appropriate physicochemical properties for application as wound dressing materials.

scholarly journals Evaluation of Efficacy of Collagen-Calcium Alginate Protectants on Chronic Wound Healing in Horses

2021 ◽  
Vol 36 (2) ◽  
pp. 116-122
Author(s):  
Khaled. M. A. Hussin ◽  
Asma Saleh W ◽  
Rehab Hamad ◽  
Marwan Saleh
Keyword(s):  
Wound Healing ◽  
Calcium Alginate ◽  
Clinical Case ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Skin Substitute ◽  
Study Results ◽  
Healing Wounds ◽  
The University ◽  
Veterinary Hospital

This study aimed to evaluate the efficacy of collagen-calcium alginate as an accelerator of wound healing in a clinical case of ten horses attended at the University Veterinary Hospital (UVH) at Omar Al-Mukhtar University, having non-healing chronic wounds. The pattern of wound healing was evaluated both clinically and by planimetry. All cases showed healing progress after the application of the collagen-calcium alginate film. The study results suggested that collagen-calcium alginate film can be a better wound healing biomaterial in horses. It can be used as a less expensive skin substitute in order to stimulate and promote wound healing in animals especially in the case of chronic non-healing wounds.

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