scholarly journals Antibacterial and In Vivo Studies of a Green, One-Pot Preparation of Copper/Zinc Oxide Nanoparticle-Coated Bandages

Membranes ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 462
Author(s):  
Archana R. Deokar ◽  
Ilana Perelshtein ◽  
Melissa Saibene ◽  
Nina Perkas ◽  
Paride Mantecca ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Metal Oxide ◽  
Ultrasound Irradiation ◽  
Face Mask ◽  
In Vivo Studies ◽  
One Pot ◽  
The Face ◽  
Bulk Scale ◽  
Particle Size And Shape

Simultaneous water and ethanol-based synthesis and coating of copper and zinc oxide (CuO/ZnO) nanoparticles (NPs) on bandages was carried out by ultrasound irradiation. High resolution-transmission electron microscopy demonstrated the effects of the solvent on the particle size and shape of metal oxide NPs. An antibacterial activity study of metal-oxide-coated bandages was carried out against Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative). CuO NP-coated bandages made from both water and ethanol demonstrated complete killing of S. aureus and E. coli bacteria within 30 min., whereas ZnO NP-coated bandages demonstrated five-log reductions in viability for both kinds of bacteria after 60 min of interaction. Further, the antibacterial mechanism of CuO/ZnO NP-coated bandages is proposed here based on electron spin resonance studies. Nanotoxicology investigations were conducted via in vivo examinations of the effect of the metal-oxide bandages on frog embryos (teratogenesis assay—Xenopus). The results show that water-based coatings resulted in lesser impacts on embryo development than the ethanol-based ones. These bandages should therefore be considered safer than the ethanol-based ones. The comparison between the toxicity of the metal oxide NPs prepared in water and ethanol is of great importance, because water will replace ethanol for bulk scale synthesis of metal oxide NPs in commercial companies to avoid further ignition problems. The novelty and importance of this manuscript is avoiding the ethanol in the typical water:ethanol mixture as the solvent for the preparation of metal oxide NPs. Ethanol is ignitable, and commercial companies are trying the evade its use. This is especially important these days, as the face mask produced by sonochemistry (SONOMASK) is being sold all over the world by SONOVIA, and it is coated with ZnO.

scholarly journals Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth

2017 ◽  
Vol 28 (5) ◽  
pp. 578-586 ◽  
Author(s):  
Katerine Jahnecke Pilownic ◽  
Ana Paula Neutzling Gomes ◽  
Zhe Jun Wang ◽  
Luiza Helena Silva Almeida ◽  
Ana Regina Romano ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Cell Viability ◽  
Primary Teeth ◽  
Antimicrobial Effect ◽  
Biological Evaluation ◽  
In Vivo Studies ◽  
Filling Materials ◽  
Histological Data ◽  
Biocompatibility Test

Abstract This study assessed the pH, radiopacity, antimicrobial effect, cytotoxicity and biocompatibility of endodontic filling materials for primary teeth. Zinc oxide eugenol (ZOE), Vitapex and Calen paste thickened with zinc oxide (ZO) were evaluated in comparison to an experimental MTA-based material. Radiopacity was tested using a graduated aluminum stepwedge with a digital sensor (n=5). The materials pH was recorded at 1, 4, 12 h; 1, 3, 7, 15 and 30 days (n=5). Direct contact test was used to assess the antimicrobial efficacy against Enterococcus faecalis after 1, 4, 12, 24 h (n=5). Cytotoxicity assay used MTT test for cell viability after incubation for 1, 3 and 7 days (n=5). For biocompatibility test, Wistar rats had received implants containing each material (n=5). The biopsied tissues were histologically analyzed after 15, 30 and 60 days. The results of radiopacity, pH, antimicrobial capacity and cytotoxicity were analyzed using ANOVA and Tukey tests. The histological data were submitted to Kruskal-Wallis test. The experimental material presented the lowest radiopacity (3.28 mm Al) and had a pH>12.0 throughout the test period. The experimental material showed the highest antibacterial effect, killing over 99.97% bacteria in 4 h. Vitapex presented the highest cell viability. Initially, biocompatibility test showed moderate to severe inflammation in all groups. After 60 days, Calen+ZO group showed moderate inflammation, while the others showed predominantly mild inflammatory reaction. The present results demonstrated that the experimental MTA-based material exhibited satisfactory behavior regarding the studied properties. Additional in vivo studies are necessary for a better evaluation of the material.

scholarly journals Nanoformulation of a Novel Pyrano[2,3-c] Pyrazole Heterocyclic Compound AMDPC Exhibits Anti-Cancer Activity via Blocking the Cell Cycle through a P53-Independent Pathway

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 624 ◽  
Author(s):  
Xuanrong Sun ◽  
Longchao Zhang ◽  
Mengshi Gao ◽  
Xiangjie Que ◽  
Chenfeng Zhou ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cancer Cells ◽  
Biological Activities ◽  
Polymeric Micelle ◽  
In Vivo Studies ◽  
One Pot ◽  
Anti Cancer ◽  
G2 Phase ◽  
Anti Tumor Activity

Pyrano[2,3-c]pyrazole derivatives have been reported as exerting various biological activities. One compound with potential anti-tumor activity was screened out by MTT assay from series of dihydropyrazopyrazole derivatives we had synthesized before using a one-pot, four-component reaction, and was named as 6-amino-4-(2-hydroxyphenyl)-3-methyl-1,4-dihydropyrano[2,3-c]pyrazole-5-carbonitrile (hereinafter abbreviated as AMDPC). The IC50 of AMDPC against Bcap-37 breast cancer cells was 46.52 μg/mL. Then the hydrophobic AMDPC was encapsulated in PEG-PLGA block copolymers, and then self-assembled as polymeric micelle (mPEG-PLGA/AMDPC) to improve both physiochemical and release profiles. The effect of mPEG-PLGA/AMDPC on BCAP-37 cancer cells showed similar anti-tumor effects as AMDPC. Furthermore, the anti-tumor mechanism of mPEG-PLGA/AMDPC was investigated, which can probably be attributed to stimulating the expression of P21 gene and therefore protein production on BCAP-37 cells, and then blocked the cell cycle through the P53-independent pathway both in S phase and G2 phase. Thus, mPEG-PLGA/AMDPC is a promising therapeutic agent for cancer treatment, and further in vivo studies will be developed.

In vitro and in vivo studies of novel fabricated bioactive dressings based on collagen and zinc oxide 3D scaffolds

2019 ◽  
Vol 557 ◽  
pp. 199-207 ◽  
Author(s):  
Paul Cătălin Balaure ◽  
Alina Maria Holban ◽  
Alexandru Mihai Grumezescu ◽  
George Dan Mogoşanu ◽  
Tudor Adrian Bălşeanu ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
In Vivo Studies ◽  
3D Scaffolds

Leak measurements in spontaneously breathing premature newborns by using the flow-through technique

1998 ◽  
Vol 85 (3) ◽  
pp. 1187-1193 ◽  
Author(s):  
B. Foitzik ◽  
M. Schmidt ◽  
D. Windstetter ◽  
R. R. Wauer ◽  
G. Schmalisch
Keyword(s):  
Measurement Errors ◽  
Face Mask ◽  
Maximum Error ◽  
Air Leaks ◽  
The Face ◽  
Flow Through ◽  
Spontaneously Breathing ◽  
Suction Flow

A new method for measuring and correcting air leaks during lung-function testing in infants has been validated in vitro and in vivo by using a flow-through system that measured the inflow and outflow of a face mask. An adjustable leak was quantified by using suction flow to validate the accuracy of leak measurements. To validate the leak correction, the volume of a pump was measured with different air leaks (0–30%). The method developed was tested in 67 infants breathing spontaneously. There was good agreement between measured and simulated leaks ( r = 0.998, P < 0.001; 95% limits of agreement were −0.3 and 0.1%, respectively). The volume was generally underestimated because of leaks, and the volume error was up to 94% compared with the maximum error of 5% after leak correction. With continuous leak measurements in vivo, there were <4% actual leaks (median 2.6%), and we did not observe any leaks in >7% of cases. The leak correction improved the accuracy of ventilatory measurements. The monitoring of leaks is helpful for airtight placement of the face mask and for prevention of serious measurement errors caused by leaks.

scholarly journals New Bioconjugated Technetium and Rhenium Folates Synthesized by Transmetallation Reaction with Zinc Derivatives

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2373
Author(s):  
Jordi Borràs ◽  
Julie Foster ◽  
Roxana Kashani ◽  
Laura Meléndez-Alafort ◽  
Jane Sosabowski ◽  
...  
Keyword(s):  
Folic Acid ◽  
Specific Binding ◽  
Zinc Complexes ◽  
In Vivo Studies ◽  
Reaction Yield ◽  
Water Medium ◽  
One Pot ◽  
Molar Activity ◽  
High Yields

The zinc dithiocarbamates functionalized with folic acid 2Zn and 3Zn were synthesized with a simple straightforward method, using an appropriated folic acid derivative and a functionalized zinc dithiocarbamate (1Zn). Zinc complexes 2Zn and 3Zn show very low solubilities in water, making them useful for preparing Tc-99m radiopharmaceuticals with a potentially high molar activity. Thus, the transmetallation reaction in water medium between the zinc complexes 2Zn or 3Zn and the cation fac-[99mTc(H2O)3(CO)3]+, in the presence of the monodentate ligand TPPTS, leads to the formation of the 2 + 1 complexes fac-[99mTc(CO)3(SS)(P)] bioconjugated to folic acid (2Tc and 3Tc). In spite of the low solubility of 2Zn and 3Zn in water, the reaction yield is higher than 95%, and the excess zinc reagent is easily removed by centrifugation. The Tc-99m complexes were characterized by comparing their HPLC with those of the homologous rhenium complexes (2Re and 3Re) previously synthesized and characterized by standard methods. Preliminary in vivo studies with 2Tc and 3Tc indicate low specific binding to folate receptors. In summary, Tc-99m folates 2Tc and 3Tc were prepared in high yields, using a one-pot transmetallation reaction with low soluble zinc dithiocarbamates (>1 ppm), at moderate temperature, without needing a subsequent purification step.

Role of Zinc Oxide Nanoparticles Synthesized by Fenugreek Seeds Extract as Anticancer Agent: In Vitro and In Vivo Studies

2021 ◽  
Vol 21 ◽  
Author(s):  
Aliaa M. Radwan ◽  
Eman F. Aboelfetoh ◽  
Tetsunari Kimura ◽  
Tarek M. Mohamed ◽  
Mai M. El-Keiy
Keyword(s):  
Zinc Oxide ◽  
Electron Microscope ◽  
Zinc Oxide Nanoparticles ◽  
In Vivo Studies ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Fenugreek Seeds ◽  
X Ray

Background: Zinc oxide nanoparticles (ZnO NPs) are one of the metal oxide nanoparticles, which have attracted the interest of the researchers due to their biocompatibility, easily surface functionalization, and cancer targeting. Objective: This study was designated to investigate the potential antitumor activity of the biologically synthesized ZnO NPs alone or in combination with doxorubicin using Ehrlich ascites carcinoma (EAC) model. Methods: In this study, ZnO NPs were prepared by green approach using fenugreek seeds extract as reducing and capping agent then characterized by scanning electron microscope (SEM), energy dispersive x-ray (EDX), X-ray diffraction (XRD), UV-V spectroscopy, and transmission electron microscope (TEM). The prepared nanoparticles were tested for in vitro and in vivo studies using different parameters. Results: Zinc oxide nanoparticles were determined to have cytotoxicity against different cancer cell lines with lower toxicity against normal one. Moreover, the in vivo study, demonstrated that the intraperitoneal injection of ZnO NPs alone or combined with doxorubicin in EAC mice inhibited the proliferation and growth of EAC by decreasing the ascetic volume and viable tumor cell count. This anti-proliferative efficiency of ZnO NPs was due to cell cycle arrest at G0/G1 phase and induction of apoptosis via upregulating the expression of caspase-3 and Bax and downregulating the expression of Bcl-2. Conclusion: Our findings indicated that the biologically synthesized ZnO NPs may be a promising nanomedicine therapy for cancer treatment in the future.

scholarly journals Design, synthesis and cytotoxic evaluation of 2-amino-4- aryl-6-substituted pyridine-3,5-dicarbonitrile derivatives

2021 ◽  
Vol 20 (10) ◽  
pp. 2127-2133
Author(s):  
Amr S. Abu Lila ◽  
Marwa H. Abdallah ◽  
El-Sayed Khafagy ◽  
Tamer M. Shehata ◽  
Mahmoud S. Soliman ◽  
...  
Keyword(s):  
Cell Lines ◽  
Condensation Reaction ◽  
In Vitro Cytotoxicity ◽  
In Vivo Studies ◽  
Primary Amines ◽  
Binding Modes ◽  
One Pot ◽  
Multicomponent Condensation

Purpose: To synthesize novel pyridine derivatives and evaluate their efficiency as potent inhibitors of cyclin dependent kinase 2 (CDK2) enzyme for cancer therapy.Methods: Pyridine scaffold were synthesized using one-pot multicomponent condensation reaction of arylidine with different primary amines. The cytotoxic potential of the new compounds was assessed using various cell lines. Furthermore, molecular docking studies based on the crystal structure of CDK2 was carried out to determine the possible binding modes that influence the anticancer activities.Results: The results indicate that one-pot multicomponent reaction generated a series of functionalized pyridines with good yield. In vitro cytotoxicity study revealed superior cytotoxicity of the designed compounds against prostate and cervical cancer cell lines compared to 5-fluorouracil (standard anticancer compound) with half-maximal inhibitory concentration (IC50) values of 0.1 – 0.85 and 1.2 –74.1 μM, respectively. Finally, molecular modeling simulation of the newly synthesized compounds showed that they fit well and are stabilized into CDK2 active site via hydrogen bonding and hydrophobic interactions.Conclusion: The results indicate that the newly synthesized pyridine can exert potent anticancer activity presumably via inhibition of CDK2. However, this will need to be confirmed in in vivo studies.

Design, Synthesis, Molecular Modeling and Anti-HIV Assay of Novel Quinazolinone Incorporated Coumarin Derivatives

2020 ◽  
Vol 18 (1) ◽  
pp. 41-51 ◽  
Author(s):  
Mahdieh Safakish ◽  
Zahra Hajimahdi ◽  
Mohammad R. Aghasadeghi ◽  
Rouhollah Vahabpour ◽  
Afshin Zarghi
Keyword(s):  
Therapeutic Index ◽  
In Vivo Studies ◽  
One Pot ◽  
Design Synthesis ◽  
Phenyl Derivative ◽  
Hiv Therapy ◽  
Anti Hiv Agents ◽  
Anti Hiv

Background: The emergence of drug-resistant viral strains has created the need for the development of novel anti-HIV agents with a diverse structure that targets key enzymes in the HIV lifecycle. Objective: Considering the pharmacophore of integrase inhibitors, one of the validated targets for anti-HIV therapy, we designed a quinazolinone incorporated coumarin scaffold to affect HIV. Method: Coumarin is a beta enol ester and also a well-known drug scaffold. Designed structures were prepared using a one-pot three-compo Results: In vitro anti-HIV and cytotoxicity assay indicated that more than half of the compounds had EC50 values lower than 50 µM. Unsubstituted phenyl derivative showed the highest activity and selectivity with an EC50 value of 5 µM and a therapeutic index of 7. Compounds were docked into the integrase active site to investigate the probable mechanism of action. Accordingly, the hydroxyl moiety of coumarin along with the carbonyl of the quinazolinone ring could function as the metal chelating group. Quinazolinone and phenyl groups interact with side chains of IN residues, as well. Conclusion: Here, a novel anti-HIV scaffold is represented for further modification and in-vivo studies.

scholarly journals Novel Small-molecule Antibacterials against Gram-positive Pathogens of Staphylococcus and Enterococcus Species

Antibiotics ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 210 ◽  
Author(s):  
Marius Seethaler ◽  
Tobias Hertlein ◽  
Björn Wecklein ◽  
Alba Ymeraj ◽  
Knut Ohlsen ◽  
...  
Keyword(s):  
Small Molecule ◽  
Pathogenic Bacteria ◽  
In Vivo Studies ◽  
Enterococcus Species ◽  
Gram Positive ◽  
One Pot ◽  
Gram Positive Bacteria ◽  
Hybrid Molecules

Defeat of the antibiotic resistance of pathogenic bacteria is one great challenge today and for the future. In the last century many classes of effective antibacterials have been developed, so that upcoming resistances could be met with novel drugs of various compound classes. Meanwhile, there is a certain lack of research of the pharmaceutical companies, and thus there are missing developments of novel antibiotics. Gram-positive bacteria are the most important cause of clinical infections. The number of novel antibacterials in clinical trials is strongly restricted. There is an urgent need to find novel antibacterials. We used synthetic chemistry to build completely novel hybrid molecules of substituted indoles and benzothiophene. In a simple one-pot reaction, two novel types of thienocarbazoles were yielded. Both indole substituted compound classes have been evaluated as completely novel antibacterials against the Staphylococcus and Enterococcus species. The evaluated partly promising activities depend on the indole substituent type. First lead compounds have been evaluated within in vivo studies. They confirmed the in vitro results for the new classes of small-molecule antibacterials.

scholarly journals Amelioration of Autoimmunity and Inflammation by Zinc Oxide Nanoparticles in Experimental Rheumatoid Arthritis

2021 ◽  
Author(s):  
Sameh Shaaban ◽  
Ahmed M. Fayez ◽  
Mahmoud Abdelaziz ◽  
Doaa Abou El-ezz
Keyword(s):  
Rheumatoid Arthritis ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Metal Oxide Nanoparticles ◽  
Severe Disease ◽  
In Vivo Studies ◽  
Total Leukocyte Count ◽  
Oxide Nanoparticles ◽  
Zno Nps

Abstract Rheumatoid arthritis (RA) is a chronic autoimmune disease that affects the lining of the synovial joints and approximately affects 0.5-1% of the total population imposing a socioeconomic burden. Currently, there is no cure for RA, but receiving proper medical care at early stages of the disease is of high importance, to prevent the progressive disability and premature death. Using rat animal model injected with Complete Freund’s adjuvant proved to be successful in induction of a state highly resembling RA in human. Zinc oxide nanoparticles (ZnO NPs) are considered as one of the most important metal oxide nanoparticles due to their exclusive properties, and they are currently merged in several biological applications due to their biocompatibility, low cost, and high safety profile. In this study, we demonstrated the novel possible beneficial effects of using zinc oxide nanoparticles, on such devastating severe disease. Zinc oxide nanoparticles (ZnO NPs) proved to reduce the adjuvant-induced increased productions of IL-1β, TNF-α, IL-10, total leukocyte count, rheumatoid factor, anti-CCP levels in rats, suggesting an interesting option to be available either alone or in combinations to better control RA.In conclusion we recommend the expansion of more in vivo studies to highlight the benefits which could be obtained of nanoparticles either alone or in combination with the known anti-arthritic and/or anti-inflammatory agents; giving rise to new protocols to maximize the control of RA.

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