scholarly journals Stress Effect of Food Matrices on Viability of Probiotic Cells during Model Digestion

2021 ◽  
Vol 9 (8) ◽  
pp. 1625
Author(s):  
Petra Matouskova ◽  
Julie Hoova ◽  
Petr Rysavka ◽  
Ivana Marova
Keyword(s):  
Stress Effect ◽  
Bacterial Strains ◽  
Fast Foods ◽  
Commercial Mixture ◽  
Artificial Digestion ◽  
Complex Protein ◽  
Effect Of Food ◽  
Food Matrices ◽  
Chocolate Spread ◽  
Positive Effect

The aim of this study was to evaluate the influence of model (alcohol, sugar, salt, protein and acid) and real foods and beverages on the viability of probiotics during incubation and artificial digestion. Viability of monocultures Lactobacillus acidophilus CCM4833 and Bifidobacterium breve CCM7825T, and a commercial mixture of 9 probiotic bacterial strains, was tested by cultivation assay and flow cytometry. In model foods, the best viability was determined in the presence of 0.2 g/L glucose, 10% albumin and 10% ethanol. As the most suitable real food for probiotic survival, complex protein and carbohydrate substrates were found, such as beef broth, potato salad with pork, chicken with rice, chocolate spread, porridge and yoghurt. The best liquid was milk and meat broth, followed by Coca-Cola, beer and coffee. Viability of probiotics was higher when consumed with meals than with beverages only. Addition of prebiotics increased the viability of probiotics, especially in presence of instant and fast foods. Generally, the highest viability of probiotics during artificial digestion was observed in mixed culture in the presence of protein, sugar and fat, or their combination. The increase of cell viability observed in such foods during model digestion may further contribute to the positive effect of probiotics on human health.

scholarly journals Stress Effect of Food Matrices on Viability of Probiotic Cells During Model Digestion

2021 ◽  
Author(s):  
Petra Matoušková ◽  
Julie Hoova ◽  
Petr Rysavka ◽  
Ivana Marova
Keyword(s):  
Stress Effect ◽  
Bacterial Strains ◽  
Fast Foods ◽  
Commercial Mixture ◽  
Artificial Digestion ◽  
Complex Protein ◽  
Effect Of Food ◽  
Food Matrices ◽  
Chocolate Spread ◽  
Positive Effect

The aim was to evaluate the influence of model (alcohol, sugar, salt, protein and acid) and real foods and beverages on the viability of probiotics during incubation and artificial digestion. Viability of monocultures Lactobacillus acidophilus CCM4833 and Bifidobacterium breve CCM7825T and commercial mixture of 9 probiotic bacterial strains were tested by cultivation assay and flow cytometry. In model foods, the best viability was determined in the presence of 0.2 g/L glucose, 10% albumin and 10% ethanol. As the most suitable real food for probiotic survival complex protein and carbohydrate substrates were found, such as beef broth, potato salad with pork, chicken with rice, chocolate spread, porridge and yoghurt. The best liquid was milk and meat broth, followed by coca-cola, beer and coffee. Viability of probiotics was higher when consumed with meals than with beverages only. Addition of prebiotics increased the viability of probiotics especially in presence of instant and fast foods. Generally, the highest viability of probiotics during artificial digestion was observed in mixed culture in presence of protein, sugar and fat or their combination. The increase of cell viability observed in such foods during model digestion may further contribute to the positive effect of probiotics on human health.

scholarly journals Pathogenetic Substantiation of the Therapeutic Impact on Microbiota in Irritable Bowel Syndrome

2019 ◽  
Vol 29 (4) ◽  
pp. 7-14
Author(s):  
Yu. O. Shulpekova ◽  
G. H. Babaeva ◽  
V. Yu. Rusyaev
Keyword(s):  
Irritable Bowel Syndrome ◽  
Intestinal Microbiota ◽  
Intestinal Bacteria ◽  
Host Cells ◽  
Intestinal Microbiome ◽  
Bacterial Strains ◽  
Blastocystis Hominis ◽  
Therapeutic Impact ◽  
Irritable Bowel ◽  
Positive Effect

Aim. This review aims to describe the nature of changes in the intestinal microbiota in irritable bowel syndrome (IBS) and provide a pathogenetic justification of the feasibility of a therapeutic impact on microbiota. General findings. An important aspect of the interaction of intestinal bacteria with the “host” cells is their contact with pattern recognition receptors of enterocytes, dendritic cell receptors, as well as a transcellular transport of antigens in the region of Peyer’s patches. The area of interaction of intestinal bacteria and the human body is not limited to the intestines. Intestinal bacteria demonstrate a significant humoral effect due to signalling molecules, some of which exhibit neurotransmitter properties. The study of the bacterial cross-feeding for various species, i.e. mutual use of nutrient substrates produced by bacteria of various species, is of a great interest. The development of a lowactivity inflammation in IBS can partly be explained by the increased interaction of flagellin with the corresponding receptor, as well as the influx of excess bacteria from the small intestine. The majority of studies on IBS have demonstrated the predominance of intestinal bacteria with pro-inflammatory potential (Enterobacteriaceae) and the lack of bacteria with a pronounced anti-inflammatory, antimicrobial and enzymatic action (Lactobacillus and Bifidobacterium), as well as increased mucus degradation. Similar changes are observed in inflammatory bowel diseases. Reduced microbial diversity increases susceptibility to intestinal infections and parasitoses, including those caused by protozoa conditionally pathogenic for adults, such as Blastocystis hominis hominis, Dientamoeba fragilis, Giardia lamblia. With the help of nutrition correction, the use of probiotics and functional foods containing certain probiotic strains, plant fibres (primarily psyllium) and, in some cases, nonabsorbable antibiotics, a positive effect can be achieved in a significant number of IBS patients. Recent works have shown that clinical improvement is accompanied by a change in the composition of the intestinal microbiota.Conclusion. For the pathogenetic treatment of irritable bowel syndrome, the use of non-drug treatment is justified, such as diet optimization and prescription of plant fibres and probiotic bacterial strains. The positive effect of such an approach is largely determined by modification of the intestinal microbiota composition. This opens up prospects for a further, more targeted impact on the intestinal microbiome.

scholarly journals Influence of Microbiologically Enriched Mineral Fertilizers on Selected Groups of Microorganisms in the Rhizosphere of Strawberry Plants

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Lidia Sas-Paszt ◽  
Urszula Smolińska ◽  
Beata Kowalska ◽  
Magdalena Szczech ◽  
Anna Lisek ◽  
...  
Keyword(s):  
Adverse Effect ◽  
Bacillus Amyloliquefaciens ◽  
Soil Microorganisms ◽  
Paenibacillus Polymyxa ◽  
Mineral Fertilizers ◽  
Bacterial Strains ◽  
Beneficial Effects ◽  
Purpureocillium Lilacinum ◽  
Bacteria And Fungi ◽  
Positive Effect

Abstract In recent years, the use of bio-fertilizers enriched with specially selected microorganisms has been used more and more often. The beneficial effects of bio-fertilizers enriched with consortia of microorganisms on strawberry plants have been reported previously. The purpose of the research was to determine the effect of bio-fertilizers containing selected fungal and bacterial strains on the microorganisms living in the rhizosphere of strawberry plants. In the experiments described in this paper, synthetic mineral fertilizers were enriched with selected microorganisms. The fertilizer urea was enriched with the fungi Aspergillus niger and Purpureocillium lilacinum, while the fertilizers Polifoska 6 and Super Fos Dar 40 with strains of the bacteria Bacillus sp., Bacillus amyloliquefaciens, and Paenibacillus polymyxa. Bacteria and fungi belonging to these species can exert a positive effect on the growth of many plants. The results obtained in this study showed that the application of fertilizers enriched with microorganisms had different effects on the analyzed populations of soil microorganisms in the rhizosphere of strawberry plants. There were evidences of both, an adverse effect of the applied fertilizer and/or microorganisms, but more often, the beneficial effect was found on the abundance of the microorganisms in the rhizosphere of the strawberry. The most effective for the population of Pseudomonas bacteria was application of urea and fungi and Polifoska and bacteria. The highest number of phosphorus utilizing bacteria B was scored in the treatments containing NPK, NPK + fungi and urea 60% + fungi. The application of NPK + fungi and urea 100% + fungi as well as Super Fos Dar with bacteria was most beneficial for population of actinomycetes.

Effect of food matrices on the in vitro bioavailability and oxidative damage in PC12 cells of lead

2018 ◽  
Vol 266 ◽  
pp. 397-404 ◽  
Author(s):  
Ji Xia ◽  
Yong Fang ◽  
Yi Shi ◽  
Xinchun Shen ◽  
Jian Wu ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Pc12 Cells ◽  
Effect Of Food ◽  
Food Matrices

The protective effect of food matrices on Listeria lytic bacteriophage P100 application towards high pressure processing

2018 ◽  
Vol 76 ◽  
pp. 416-425 ◽  
Author(s):  
Norton Komora ◽  
Carolina Bruschi ◽  
Vânia Ferreira ◽  
Cláudia Maciel ◽  
Teresa R.S. Brandão ◽  
...  
Keyword(s):  
High Pressure ◽  
Protective Effect ◽  
High Pressure Processing ◽  
Lytic Bacteriophage ◽  
Effect Of Food ◽  
Food Matrices

scholarly journals Effect of food shortage and metal contamination on the mealworm Tenebrio molitor (Coleoptera: Tenebrionidae)

2019 ◽  
Vol 116 ◽  
pp. 00100
Author(s):  
Magdalena Wróbel ◽  
Justyna Rybak
Keyword(s):  
Lipid Content ◽  
Metal Contamination ◽  
Food Shortage ◽  
Metabolic Reaction ◽  
Great Decrease ◽  
Additional Source ◽  
Cu Contamination ◽  
Effect Of Food ◽  
Positive Effect ◽  
Coleoptera Tenebrionidae

In this study we assessed the metabolic reaction of mealworms to toxic metals Cu and Pb alone or delivered with carrots as an additional source of water and minerals. We compared our results with metabolic reaction of mealworms to food shortage. We recorded the highest morality and mass loss in culture contaminated with Pb (in relation to Cu contamination) proving the greatest impact of this metal on metabolism of mealworms. The great decrease in protein, carbohydrates and lipid content was observed in this variant (mealworms fed with food contaminated with Pb) as well. The slight positive effect of carrot addition was observed for larvae fed with both metals. Starvation had similar but slighter effect as Pb on metabolism as starved larvae were characterised by high, but not the highest decrease of protein, carbohydrates and lipid content. The studies proved that metal contamination could have great impact on metabolism of mealworms which is a key issue when we consider these insects as a source of proteins in place of vertebrates.

scholarly journals Microbes Mediated Mitigation of Abiotic Stresses in Agriculture

2021 ◽  
Author(s):  
S.S. Bobade ◽  
S.P. Dhekane ◽  
P.A. Salunke ◽  
S.G. Mane ◽  
S.S. Dhawan ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Abiotic Stresses ◽  
Heavy Metal Contamination ◽  
Crop Yields ◽  
Wheat Plant ◽  
Soil Samples ◽  
Pot Experiment ◽  
Bacterial Strains ◽  
Randomized Design ◽  
Positive Effect

Background: Crop yields are limited by major biotic and abiotic stresses. Various studies had been suggested that abiotic stresses like drought, flood and salinity play a major role in limiting crop yield. Heavy metal contamination is also a major problem in the agriculture sector.Methods: A pot experiment was conducted to elucidate the effect of inoculating bacterial strains on the wheat plant under various stress conditions. The bacteria were isolated and screened from drought, flood and heavy metal stressed soil samples. The selected strains were identified by morphological, biochemical and molecular methods. The ability of Acinetobacter junni S1, Acinetobacter junni S2, Leclercia adecarboxylata and Klebsiella variicola to stimulate the growth of plants were determined by pot experiment using a completely randomized design. The positive effect of isolates on seed germination percent, shoot and root length of the treated wheat plant were recorded. Analysis of soil samples from pots was carried out for evaluation of the presence of macro and micronutrients. Result: The pots inoculated with selected isolates showed a significant increase in pH 7.77, EC 2.11, carbon 0.78, nitrogen 30.83 kg/ha, phosphorus 2.95 kg/ha, potassium 535.32 kg/ha, zinc 0.15 ppm, manganese 0.376 ppm, iron 0.53 ppm and copper 0.15 ppm as compared to control. The chlorophyll content estimation was carried out by using Arnon’s method. The chlorophyll a, b and total chlorophyll was found to be 14.39, 39.74 and 38.75 respectively.

scholarly journals Protein Food Matrix–ZnO Nanoparticle Interactions Affect Protein Conformation, but May not Be Biological Responses

2018 ◽  
Vol 19 (12) ◽  
pp. 3926 ◽  
Author(s):  
Song-Hwa Bae ◽  
Jin Yu ◽  
Tae Lee ◽  
Soo-Jin Choi
Keyword(s):  
Cellular Uptake ◽  
Structural Deformation ◽  
Food Matrix ◽  
Digestive Efficiency ◽  
Protein Matrix ◽  
Zno Nps ◽  
Protein Food ◽  
Biological Responses ◽  
Complex Protein ◽  
Food Matrices

Because of their nutritional value, zinc oxide (ZnO) nanoparticles (NPs) are applied as a dietary source of zinc, by direct addition to complex, multiple-component food matrices. The thereby occurring interactions of NPs with food matrices may have biological or toxic effects. In particular, NP interactions with food protein can lead to structural deformation of the latter, potentially changing its digestive efficiency and gastrointestinal absorption. In this study, interactions between ZnO NPs and a representative complex protein food matrix, skim milk, were compared with those between NPs and individual components of this food matrix (i.e., protein, saccharide, and mineral). The effects of the interactions on biological responses were investigated in terms of cytotoxicity, cellular uptake, intestinal transport, structural deformation for proteins, and digestive efficiency. The results demonstrated that the physicochemical properties of ZnO NPs were strongly influenced by the protein matrix type, leading to an increased dispersion stability in the complex protein matrix. However, these interactions did not affect cell proliferation, membrane damage, cellular uptake, intestinal transportation, or protein digestive efficiency, although a slight conformational change of proteins was observed in the presence of ZnO NPs. In conclusion, no toxic effects were observed, suggesting the safety of NPs when added to complex food matrices.

scholarly journals Effect of Food Consumption Values of Vietnamese Consumers on the Reliability and Purchase Intention of Korean Home Meal Replacements

2022 ◽  
Vol 14 (2) ◽  
pp. 736
Author(s):  
Seunggyun Choi ◽  
Timothy J. Lee ◽  
Wansoo Hong
Keyword(s):  
Factor Analysis ◽  
Food Consumption ◽  
Purchase Intention ◽  
Time Saving ◽  
Intention To Purchase ◽  
Consumption Values ◽  
Confirmatory Factor ◽  
Effect Of Food ◽  
Positive Effect ◽  
Meal Replacements

The Vietnamese home meal replacement (HMR) market is expected to face intensified competition due to economic development and urbanization. This study analyzes how the food consumption values of Vietnamese consumers affect the perceived reliability and intention to purchase Korean food in the form of HMRs and provides basic data to establish strategies for Korean HMRs to secure an advantage in an intensely competitive market. A survey was conducted with Vietnamese consumers who had used Korean HMRs before and are constantly using HMRs on a regular basis. To analyze the relationships between food consumption values, the reliability of Korean HMR, and the intention to purchase Korean HMRs, exploratory factor analysis, reliability analysis, confirmatory factor analysis, and covariance structural models were used. Among the factors of food consumption value, health, safety, time saving, and convenience have a positive effect on the reliability of Korean HMRs, while family and cost-effectiveness do not. Moreover, this reliability has a positive effect on the purchase intention of Korean HMRs. This study is significant in that while investigating Korean HMRs, it is pioneering research on Vietnamese consumers regarding HMRs. Therefore, the results of this study can be used to secure the competitiveness of Korean HMRs in the Vietnamese market.

Neurochemical study of the mechanism of action of thymus peptides in emotional stress

2020 ◽  
Vol 26 (4) ◽  
Author(s):  
Anna V. Novoseletskaya ◽  
Nina M. Kiseleva
Keyword(s):  
High Performance Liquid Chromatography ◽  
Frontal Cortex ◽  
Emotional Stress ◽  
High Performance ◽  
Wistar Rats ◽  
Avoidance Reaction ◽  
Stress Effect ◽  
The Brain ◽  
Positive Effect ◽  
Monoamines And Their Metabolites

A comparative study of the effect of the thymus hormone thymulin and thymus peptides (thymosin fraction 5) on the content of monoamines and their metabolites in the frontal cortex, striatum, adjacent nucleus, hypothalamus, hippocampus of the brain of Wistar rats, by high performance liquid chromatography, was performed. The hormone and peptides of the thymus were found to reduce emotional stress during functional impairment ofthe avoidance reaction and improved adaptation under stressful conditions in rats, which indicates the anti-stress effect of thymus hormones. The positive effect of the hormone and peptides of the thymus were manifested in a change in the balance of serotonin and norepinephrine in favor of the former in the hypothalamus and frontal cortex.

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