scholarly journals Multilocus Genotyping of ‘Candidatus Phytoplasma solani’ Associated with Rubbery Taproot Disease of Sugar Beet in the Pannonian Plain

2021 ◽  
Vol 9 (9) ◽  
pp. 1950
Author(s):  
Živko Ćurčić ◽  
Andrea Kosovac ◽  
Jelena Stepanović ◽  
Emil Rekanović ◽  
Michael Kube ◽  
...  

Rubbery taproot disease of sugar beet (RTD), associated with ‘Candidatus Phytoplasma solani’, appeared in 2020 on an epidemic scale in northern Serbia and southern Slovakia, situated at opposite edges of the Pannonian Plain. In the affected locations where the disease was assessed, symptomatic sugar beets were analysed for phytoplasma infection. Additionally, multilocus sequence analyses of ‘Ca. P. solani’ strains on epidemiologically informative marker genes (tuf, stamp and vmp1) were performed. Symptomatic sugar beets from other countries of the Pannonian Plain (Croatia, Hungary and Austria), one sample from Germany, and red beets from Serbia were included in the analyses. ‘Ca. P. solani’ was detected in sugar beet in all assessed countries, as well as in red beet. Molecular analyses revealed the high genetic variability of ‘Ca. P. solani’ with the presence of all four tuf-types (a, b1, b2 and d), 14 stamp genotypes (seven new) and five vmp1 profiles (one new). The most common multilocus genotype in Serbia, Slovakia, Croatia, and Hungary was dSTOLg (tuf-d/STOL/V2-TA). It was dominant on sites with epidemic RTD outbreaks in the Pannonian Plain and in several sugar beet fields with non-epidemic RTD occurrence suggesting the prevalence of a particular epidemiological pathway during the epidemic’s phases.

2016 ◽  
pp. 517-524 ◽  
Author(s):  
Martin Wegener ◽  
Natalie Balgheim ◽  
Maik Klie ◽  
Carsten Stibbe ◽  
Bernd Holtschulte

KWS SAAT SE and Bayer CropScience AG are jointly developing and commercializing an innovative system of weed control in sugar beet for the global market under the name of CONVISO SMART. The technology is based on the breeding of sugar beet cultivars that are tolerant to herbicides of the ALS-inhibitor-class with a broad-spectrum weed control. This will give farmers a new opportunity to make sugar beet cultivation easier, more flexible in its timing and more efficient. The use of CONVISO, as new herbicide in sugar beet, will make it possible to control major weeds with low dose rates of product and reduced number of applications in the future. The tolerance is based on a change in the enzyme acetholactate synthase, which is involved in the biosynthesis of essential amino acids. This variation can occur spontaneously during cell division. During the development, sugar beets with this spontaneously changed enzyme were specifically selected and used for further breeding of CONVISO SMART cultivars. As such, these varieties are not a product of genetic modification. Field studies with CONVISO SMART hybrids showed complete crop selectivity and a broad and reliable efficacy against a large range of major weeds. The bio-dossier for an EU-wide registration of CONVISO was submitted in April in 2015. The variety inscription process is in preparation in different countries. The system CONVISO SMART is scheduled to be available to farmers in 2018 at the earliest.


2014 ◽  
pp. 228-231 ◽  
Author(s):  
Maciej Wojtczak ◽  
Aneta Antczak-Chrobot ◽  
Edyta Chmal-Fudali ◽  
Agnieszka Papiewska

The aim of the study is to evaluate the kinetics of the synthesis of dextran and other bacterial metabolites as markers of microbiological contamination of sugar beet.


2020 ◽  
Vol 69 (1-2) ◽  
pp. 1-4
Author(s):  
Milijanka Balandžić ◽  
Vera Stojšin ◽  
Mila Grahovac ◽  
Ferenc Bagi ◽  
Mladen Petreš ◽  
...  

SummarySugar beet leaf spot, caused by the air-borne fungus Cercospora beticola Sacc., leads to a decrease in sugar beet leaf mass and the consequent regrowth of leaves based on exploiting the sugar reserves stored in the plant’s roots, thus ultimately resulting in lower yields and sugar contents of sugar beets. Azoxystrobin belongs to the group of QoI fungicides, which inhibit mitochondrial respiration by blocking cytochrome c reductase. The QoI fungicides are characterized by a very high risk of resistance interfering with their biological activity. For the purpose of testing the azoxystrobin sensitivity of the Cercospora beticola population found at the site of Rimski Šančevi, a collection of 84 isolates was assembled and tested for sensitivity to azoxystrobin by measuring the mycelial growth on fungicide-amended media with the addition of SHAM. The results obtained indicate that none of the isolates tested exhibited complete sensitivity to azoxystrobin, 4% were found to have reduced sensitivity, 26% were moderately resistant and 70% were highly resistant. A higher proportion of resistant isolates recorded is associated with the loss of azoxystrobin biological efficacy at the experimental site.


2013 ◽  
Vol 54 (1) ◽  
pp. 163-173
Author(s):  
Marian Wesołowski ◽  
Cezary Kwiatkowski

The effect of the number of mechanical operations in sugar beets plantation on the amount and species composition of weed seeds in the 0-5 cm deep layer of the loessial soil was studied. It has been proved that reduction in the number of weed seeds depends upon both the frequency of weeding-out operations and the level of agrotechnic. The highest decrease in the number of fruit and weed seeds was caused by eightfold weed removal which took place during the period from emergence phase to the joining of sugar beet rows. Application of increased mineral fertilization, microelements, fungicides, and insecticides caused the number of weed seeds to be reduced by 5,9%, in comparison to extensive agrotechnical level.


2021 ◽  
Vol 36 ◽  
pp. 03017
Author(s):  
A.S. Avilov ◽  
N.Ya. Shmyreva ◽  
A.A. Zavalin ◽  
O.A. Sokolov

The studies were carried out under the conditions of a microfield experiment on typical chernozem (Belgorod region) with two soils (pH 5.0 and 6.5) with the addition of urea (enriched in 15N, 17.1 at. %) And effluents from a pig-breeding complex (PSC) at doses of 6 and 12 g N/m2 (against the background of phosphate and potash fertilizers, P6K6). The largest amount of nitrogen was consumed by sugar beets with the combined introduction of CCA and urea (25.8 g N/m2). When the soil was acidified to pHsalt 5.0, the consumption of nitrogen in mineral fertilizers decreased by 15-18%, soil ni-trogen – by 21-52%, and waste nitrogen – by 16%. At the same time, the immobilization of nitrogen in the fertilizer decreased by 13-18%, and the loss of gaseous nitrogen compounds increased by 47-108%. The effluent from the pig-breeding complex increased the immobilization of nitrogen in mineral fertilizers (by 38-46%) and reduced gaseous nitrogen losses (by 22-44%). The highest stability and productivity (root crop yield 1654 g/m2, tops yield 239 g/m2) was exhibited by agrophytocenosis on soil with pH 6.5 with the combined application of CCA and urea. When the soil solution was acid-ified to pH 5.0, the yield of beet root crops decreased by 30% and the yield of tops – by 24%. On soil with pH 5.0, fertilizers increased the sugar content in root crops by 0.2-1.3%, on soil with pH 6.5, they decreased by 1.3-2.0%.


Author(s):  
Lubov Belyaeva ◽  
Michail Pruzhin ◽  
Alla Ostapenko ◽  
Valentina Gurova

Introduction. Technological aids play a special role in sugar production technology, but their complex effectiveness requires a comprehensive and in-depth study. The research objective was to establish the patterns of change in the technological indicators of semi-finished products obtained from bacterially infected sugar beets with the combined use of an enzyme preparation, antimicrobial agent, and defoamer. Study objects and methods. The study involved such semi-finished products as juice (diffusion, pre-defecated, first and second saturation) and syrup, the quality of which was determined according to standard methods. The laboratory experiment was carried out on the basis of the second-order D-optimal Box-Behnken plan for three factors at three levels. Results and discussion. The research revealed positive dynamics of the following technological indicators: sucrose content, deposition rate, turbidity, chromaticity, and general purification effect. The sugar beet had the second degree of infection with mucous bacteriosis. Purified juice underwent lime-carbon dioxide purification and thickening under the combination of enzyme preparation Dextrasept 2, antimicrobial agent Betasept, and antifoam agent Voltes FSS 93. The greatest increase in sucrose at the level of 1.1% by DM weight was confirmed by a higher overall effect of purification of diffusion juice (2.2 %). The values of turbidity of the purified juice and syrup were below the threshold values. The low values resulted from the increase in the sedimentation rate of the pre-defective juice and the juice of the first saturation by an average of 4.1 and 3.2 times, respectively, due to the effective removal of high molecular weight compounds. The share of the enzyme preparation was 40–71%, antimicrobial agent – 19–49%, defoamer – 1.6–6.5%. The values of the multicriteria optimization parameter corresponded with technological indicators. The optimal combination (per 1000 tons of beets) included 6–8 kg of Dextrasept 2, 1.5–2.0 kg of Betasept, and 15–20 kg of Voltes FSS 93. As a result, the yield of white sugar increased by 0.25%. Conclusion. The regression dependencies can be recommended for predicting the main technological indicators of semi-finished products. The resulting data makes it possible to determine the effectiveness of the combined use of an enzyme preparation, antimicrobial agent, and defoamer in sugar production. Further research will identify the patterns of multifactorial interaction of these preparations.


2012 ◽  
Vol 58 (No. 1) ◽  
pp. 41-48 ◽  
Author(s):  
J. Pulkrábek ◽  
M. Kavka ◽  
V. Rataj ◽  
J. Humpál ◽  
L. Nozdrovický ◽  
...  

The risk rate of sugar beet growing was analyzed by using the algorithm for modelling and generating the random numbers based on the conditions determined in advance and the statistical distribution of sugar beets yield, the total costs, earnings and gross profit. On the bases of results of the analysis of economic risks of sugar beet production in years 1995–2009, it follows that the sugar beet growing in all regions is highly risky in the Czech Republic. The subsidy SAPS and TOP-UP per 1 hectare of sugar beet have a positive impact on its economics though not sufficient enough. In practice, it means that it is highly probable that the break-even point will not be achieved and thereby it is highly probable that the fixed costs are not covered and the update of machines is significantly limited. This happens mainly in the marginal regions. If the agricultural companies count on the separate sugar payment, the sugar beet growing is in all regions of the Czech Republic with acceptable risk.  


1995 ◽  
Vol 129 (4) ◽  
pp. 1081-1092 ◽  
Author(s):  
A J Otsuka ◽  
R Franco ◽  
B Yang ◽  
K H Shim ◽  
L Z Tang ◽  
...  

Caenorhabditis elegans unc-44 mutations result in aberrant axon guidance and fasciculation with inappropriate partners. The unc-44 gene was cloned by transposon tagging, and verified by genetic and molecular analyses of six transposon-induced alleles and their revertants. Nucleotide sequence analyses demonstrated that unc-44 encodes a series of putative ankyrin-related proteins, including AO49 ankyrin (1815 aa, 198.8 kD), AO66 ankyrin (1867 aa, 204 kD), and AO13 ankyrin (< or = 4700 aa, < or = 517 kD). In addition to the major set of approximately 6 kb alternatively spliced transcripts, minor transcripts were observed at approximately 3, 5, 7, and 14 kb. Evidence is provided that mutations in the approximately 14-kb AO13 ankyrin transcript are responsible for the neuronal defects. These molecular studies provide the first evidence that ankyrin-related molecules are required for axonal guidance.


2018 ◽  
Vol 44 ◽  
pp. 00114 ◽  
Author(s):  
Natalia Mioduszewska ◽  
Mariusz Adamski ◽  
Anna Smurzyńska ◽  
Jacek Przybył ◽  
Krzysztof Pilarski

The aim of the study was to evaluate the usefulness of sugar beet for biogas production, taking into account the duration time of storage and sugar content in the roots. The research has included analysis of methane and biogas yield of sugar beet. The relations between the sugar content in the roots and the length of storage period and the course of the methane fermentation process were determined. Sugar beets with sugar content of 17.6% and 19.6% were used for this experiment. In order to analyse the fermentation process, the fresh beets and the beets stored in flexible, hermetic tanks in the period of 43 and 89 days were used. Based on the analysis of the obtained results, it was found that the sugar content and the storage time of sugar beet roots can differentiate the production of biomethane and that it influences the methane fermentation process and the quality of the produced biogas.


Processes ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 1402
Author(s):  
Andrzej Baryga ◽  
Bożenna Połeć ◽  
Andrzej Klasa

Management of digestate from production of biogas has a great environmental importance. One of feedstock for biogas generation is beet pulp, a side product of sugar beet processing plant. In the paper a closed loop of beet pulp utilization at sugar beet plantation is presented. Effects of soil application of digestate obtained from digestion of sugar beet pulp were compared with standard mineral fertilizers. The field experiment was performed in three successive growing seasons. The studies were concentrated on quality of sugar beets grown under effects of two fertilization treatments—soil application of digestate cv. standard mineral fertilizers. It was found that some important quality indices (weight of single sugar beet root, content of sucrose in root tissues) were higher for beet harvested from digestate treatment compared to standard mineral fertilization (control). The concentration of harmful component (amide nitrogen) in sugar beets grown under conditions of digestate soil application was lower than in the control. It can be concluded that soil application of digestate from processing of sugar beet pulp can be treated as environmentally sound and effective method of its management.


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