scholarly journals Gallic Acid-Chitosan Conjugate Inhibits the Formation of Calcium Oxalate Crystals

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2074 ◽  
Author(s):  
Moacir Fernandes Queiroz ◽  
Karoline Rachel Teodosio Melo ◽  
Diego Araujo Sabry ◽  
Guilherme Lanzi Sassaki ◽  
Hugo Alexandre Oliveira Rocha ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Gallic Acid ◽  
Reducing Power ◽  
Renal Calculus ◽  
Control Group ◽  
Copper Chelation ◽  
Total Antioxidant ◽  
Calculus Formation ◽  
Caox Crystal

It has recently been shown that chitosan (Chit) induces the formation of calcium oxalate (CaOx) crystals, which are mainly responsible for the appearance of kidney stones, and this might limit the use of Chit in vivo. Here, Chit was conjugated with gallic acid (Chit-Gal) to decrease the formation of CaOx crystal. This conjugation was confirmed by FTIR and NMR analyses. Chit-Gal contains 10.2 ± 1.5 mg GA per g of Chit. Compared to the control group, Chit increased the number of crystals by six-fold, mainly in the number of monohydrated CaOx crystals, which are the most harmful CaOx crystals. In addition, Chit increased the zeta potential (ζ) of CaOx crystals by three-fold, indicating that Chit was associated with the crystals. These alterations were abolished when Chit-gal was used in these tests. As oxidative stress is related to renal calculus formation, Chit and Chit-Gal were also evaluated as antioxidants using total antioxidant Capacity (TAC), reducing power, ferrous chelation, and copper chelation tests. Chit-gal was more efficient antioxidant agent in TAC (2 times), in ferrous chelation (90 times), and in reducing Power (5 times) than Chit. Overall, Chit-gal has higher antioxidant activity than Chit, does not induce the formation of CaOx crystals. Thus, Chit-Gal has potential to be used as a chit substitute.

scholarly journals In vitro Antioxidant Activity of Artemisia argyi Powder and the Effect on Hepatic and Intestinal Antioxidant Indices in Broiler Chickens

2020 ◽  
Vol 20 (3) ◽  
pp. 1085-1099
Author(s):  
Pengfei Zhang ◽  
Hongyan Chen ◽  
Binlin Shi ◽  
Fei Zhao ◽  
Xiaoyu Guo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Broiler Chickens ◽  
Reducing Power ◽  
Antioxidant Defenses ◽  
Control Group ◽  
Sod Activity ◽  
Artemisia Argyi ◽  
Total Antioxidant

AbstractThis study was conducted to investigate the in vitro and in vivo antioxidant effect of Artemisia argyi powder (AAP). 240 mixed-sex one-day-old Arbor Acres broilers were randomly divided into five treatment groups, each consisting of six replicates (one replicate per cage) with eight broilers per replicate. Broilers were fed basal diets supplemented with 0, 2.5, 5, 10 and 20 g AAP per kg feed, respectively. The hepatic and intestinal samples were collected on d 21 and 42 for analysis of antioxidant indices and antioxidative enzyme gene expression. The in vitro results showed that the scavenging activity of Artemisia argyi against •OH and DPPH were 34.99±1.11% and 74.12±0.50%, respectively; the ferric reducing power was 2.58±0.03%. The in vivo results showed that dietary 20 g/kg of AAP significantly enhanced the hepatic total antioxidant capacity (T-AOC), catalase (CAT) activity, and glutathione peroxidase (GSH-Px) activity, also decreased the malondialdehyde (MDA) content; dietary10 g/kg of AAP significantly increased the gene expression of superoxide dismutase (SOD) and CAT on d 42. For the duodenum, 10 g/kg of AAP increased SOD activity (P<0.05), and reduced MDA level (P<0.05) on d 21; the gene expression of CAT and SOD were increased in the 20 g/kg of AAP treatment compared with the control group on d 42. For the jejunum, on d 21, the T-AOC level was increased by inclusion of 10 g/kg of AAP, and CAT activity was enhanced significantly at 5, 10, and 20 g/kg of AAP group; dietary AAP significantly decreased MDA level at the concentration of 2.5, 5, 10 and 20 g/kg in contrast with control group on d 42; 5 and 20 g/kg of AAP increased the gene expression of SOD on d 21, and the gene expression of GSH-Px was increased (P<0.05) in 10 g/kg of AAP group on d 42. For the ileum, compared to the control group, 2.5 and 20 g/kg of AAP increased SOD activity (P<0.05); and dietary 10 and 20 g/kg of AAP significantly reduced MDA level; dietary 10 g/kg of AAP increased the gene expression of SOD, CAT and GSH-Px in broilers on d 42. In conclusion, dietary AAP could improve the antioxidant defenses of liver and small intestine, and the best concentration of the AAP improving hepatic and small intestinal antioxidant status was 20 g/kg and 10 g/kg, respectively.

scholarly journals Gallic Acid-Laminarin Conjugate Is a Better Antioxidant than Sulfated or Carboxylated Laminarin

Antioxidants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1192
Author(s):  
Marília Medeiros Fernandes-Negreiros ◽  
Lucas Alighieri Neves Costa Batista ◽  
Rony Lucas Silva Viana ◽  
Diego Araujo Sabry ◽  
Almino Afonso Oliveira Paiva ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Antioxidant Activities ◽  
Mdck Cells ◽  
Brown Seaweed ◽  
Reducing Power ◽  
Lobophora Variegata ◽  
Copper Chelation ◽  
Total Antioxidant

A 12.4 kDa laminarin (LM) composed of β(1→3)-glucan with β(1→6)-branches was extracted from brown seaweed Lobophora variegata and modified via carboxylation using dielectric barrier discharge (LMC), conjugation with gallic acid (LMG), and sulfation (LMS). Analyses of the chemical composition of LMC, LMG, and LMS yielded 11.7% carboxyl groups, 1.5% gallic acid, and 1.4% sulfate content, respectively. Antioxidant activities of native and modified laminarins were assessed using six different in vitro methods. Sulfation stopped the antioxidant activities of LM. On the other hand, carboxylation improved cooper chelation (1.2 times). LMG was found to be a more efficient antioxidant agent than LM in terms of copper chelation (1.3 times), reducing power (1.3 times), and total antioxidant capacity (80 times). Gallic acid conjugation was further confirmed using Fourier transform infrared spectroscopy (FT-IR) and one- and two-dimensional NMR spectroscopy analyses. LMG also did not induce cell death or affect the cell cycle of Madin–Darby canine kidney (MDCK) cells. On the contrary, LMG protected MDCK cells from H2O2-induced oxidative damage. Taken together, these results show that LMG has the potent antioxidant capacity, and, therefore, potential applications in pharmacological and functional food products.

scholarly journals The Protective Roles of Estrogen Receptor β in Renal Calcium Oxalate Crystal Formation via Reducing the Liver Oxalate Biosynthesis and Renal Oxidative Stress-Mediated Cell Injury

2019 ◽  
Vol 2019 ◽  
pp. 1-17 ◽  
Author(s):  
Wei Zhu ◽  
Zhijian Zhao ◽  
Fu-Ju Chou ◽  
Li Zuo ◽  
Tongzu Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Estrogen Receptor ◽  
Nadph Oxidase ◽  
Calcium Oxalate ◽  
Cell Lines ◽  
Ros Production ◽  
Crystal Deposition ◽  
Caox Crystal

Females develop kidney stones less frequently than males do. However, it is unclear if this gender difference is related to altered estrogen/estrogen receptor (ER) signaling. Here, we found that ER beta (ERβ) signals could suppress hepatic oxalate biosynthesis via transcriptional upregulation of the glyoxylate aminotransferase (AGT1) expression. Results from multiple in vitro renal cell lines also found that ERβ could function via suppressing the oxalate-induced injury through increasing the reactive oxygen species (ROS) production that led to a decrease of the renal calcium oxalate (CaOx) crystal deposition. Mechanism study results showed that ERβ suppressed oxalate-induced oxidative stress via transcriptional suppression of the NADPH oxidase subunit 2 (NOX2) through direct binding to the estrogen response elements (EREs) on the NOX2 5′ promoter. We further applied two in vivo mouse models with glyoxylate-induced renal CaOx crystal deposition and one rat model with 5% hydroxyl-L-proline-induced renal CaOx crystal deposition. Our data demonstrated that mice lacking ERβ (ERβKO) as well as mice or rats treated with ERβ antagonist PHTPP had increased renal CaOx crystal deposition with increased urinary oxalate excretion and renal ROS production. Importantly, targeting ERβ-regulated NOX2 with the NADPH oxidase inhibitor, apocynin, can suppress the renal CaOx crystal deposition in the in vivo mouse model. Together, results from multiple in vitro cell lines and in vivo mouse/rat models all demonstrate that ERβ may protect against renal CaOx crystal deposition via inhibiting the hepatic oxalate biosynthesis and oxidative stress-induced renal injury.

scholarly journals Ajuga Bracteosa Transgenic Regenerants Display Better Pharmacological Potential

2021 ◽  
Author(s):  
Samina Rubnawaz ◽  
Waqas Khan Kayani ◽  
Nosheen Akhtar ◽  
Rashid Mahmood ◽  
Furrukh Mehmood ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Abnormal Behavior ◽  
Mice Model ◽  
Hydroxyl Ion ◽  
Total Antioxidant ◽  
Endangered Medicinal Herb

Abstract Ajuga bracteosa Wall. ex Benth is an endangered medicinal herb used against different ailments in folklore medicines. Here, we aimed to create a new insight to the fundamental mechanisms of genetic transformation in the ethnomedicinal usage of this plant. We transformed the plant with rol genes of Agrobacterium rhizogenes and raised the regenerants from the hairy roots. The transgenic regenerants were screened for in vitro antioxidant activities, a range of in vivo assays, and linked the activities with elemental analysis, polyphenol content and different phytochemicals found through HPLC. Among 18 polyphenolic standards, kaempferol was found most abundant in all transgenic lines (up to 101.26 ± 6 µg/mg). Furthermore, among all tested plant extracts, transgenic line 3 (ABRL3) showed maximum phenolics (13.39 ± 2µg GAE/mg) and flavonoids content (4.75 ± 0.16 µg QE/mg). ABRL3 also demonstrated potent total antioxidant capacity (8.16 ± 1 µg AAE/mg), total reducing power, (6.60 ± 1.17 µg AAE/mg), DPPH activity (IC50 = 59.5 ± 0.8µg/mL), hydroxyl ion scavenging (IC50 = 122.5 ± 0.90 µg/mL), and iron chelating power (IC50 = 154.8 ± 2 µg/mL) among all plants. Transformed plant extracts also produced significant analgesic, anti-inflammatory, anticoagulant, and antidepressant properties in in vivo mice model as compared to control untransformed plant material. Additionally, no abnormal behavior or lethality was observed in any animal tested. In conclusion, transgenic regenerants of A. bracteosa pose better pharmacological properties under the effect of rol genes as compared to wild type plants.

Metformin protects against diabetes–induced heart injury and dunning prostate cancer model

2020 ◽  
pp. 096032712094745
Author(s):  
BB Bayrak ◽  
P Koroglu ◽  
O Karabulut Bulan ◽  
R Yanardag
Keyword(s):  
Prostate Cancer ◽  
Tissue Damage ◽  
Total Antioxidant Status ◽  
Experimental Period ◽  
Heart Tissue ◽  
Diabetic Rats ◽  
Control Group ◽  
Cancer Group ◽  
Total Antioxidant

In this study, both diabetes and Dunning prostate cancer were induced for the first time in Copenhagen rats in vivo. Thus, the effects of metformin against heart tissue damage of these rats were investigated by biochemical methods. Dunning prostate cancer was induced in Copenhagen rats using high metastatic MAT-LyLu cells. The rats were divided as follows: Control group: only injected with 0.9% NaCl for 14 days; Diabetic group: only injected single dose of streptozotocin (STZ) (65 mg/kg); Cancer group: subcutaneously (s.c) inoculated with 2 x 104 MAT-LyLu cells only; Diabetic + cancer (DC) group: inoculated with 2 x 104 MAT-LyLu cells and STZ injection, Cancer + metformin (CM) group: injected with metformin for 14 days after Mat-LyLu cells application; Diabetic + cancer + metformin (DCM) group: metformin administered for 14 days together with STZ and Mat-LyLu cells. At the end of the experimental period, heart tissues were taken. Reduced glutathione and total antioxidant status levels in heart tissues were decreased, whereas lipid peroxidation, advanced oxidized protein products, nitric oxide, homocysteine, and reactive oxygen species levels, total oxidant status and catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and xanthine oxidase activities increased in the diabetic, cancer and DC groups. Treatment with metformin reversed these effects. In conclusion, the present study shows that metformin has a protective effect against heart tissue damage in STZ-induced diabetic rats with Dunning prostate cancer.

scholarly journals Lactiplantibacillus plantarum Reduced Renal Calcium Oxalate Stones by Regulating Arginine Metabolism in Gut Microbiota

2021 ◽  
Vol 12 ◽  
Author(s):  
Yu Liu ◽  
Xi Jin ◽  
Lei Tian ◽  
Zhongyu Jian ◽  
Yucheng Ma ◽  
...  
Keyword(s):  
Drinking Water ◽  
Gut Microbiota ◽  
Calcium Oxalate ◽  
Control Group ◽  
Arginine Metabolism ◽  
Model Group ◽  
Crystal Deposition ◽  
Caox Crystal ◽  
Relative Abundances

Renal calcium oxalate (CaOx) stones are a common kidney disease. There are few methods for reducing the formation of these stones. However, the potential of probiotics for reducing renal stones has received increasing interest. We previously isolated a strain of Lactiplantibacillus plantarum N-1 from traditional cheese in China. This study aimed to investigate the effects of N-1 on renal CaOx crystal deposition. Thirty rats were randomly allocated to three groups: control group (ddH2O by gavage), model group [ddH2O by gavage and 1% ethylene glycol (EG) in drinking water], and Lactiplantibacillus group (N-1 by gavage and 1% EG in drinking water). After 4 weeks, compared with the model group, the group treated with N-1 exhibited significantly reduced renal crystals (P &lt; 0.05). In the ileum and caecum, the relative abundances of Lactobacillus and Eubacterium ventriosum were higher in the control group, and those of Ruminococcaceae UCG 007 and Rikenellaceae RC9 were higher in the N-1-supplemented group. In contrast, the relative abundances of Staphylococcus, Corynebacterium 1, Jeotgalicoccus, Psychrobacter, and Aerococcus were higher in the model group. We also predicted that the arginase level would be higher in the ileal microbiota of the model group than in the N-1-supplemented group with PICRUSt2. The arginase activity was higher, while the level of arginine was lower in the ileal contents of the model group than in the N-1-supplemented group. The arginine level in the blood was also higher in the N-1-supplemented group than in the model group. In vitro studies showed that exposure to arginine could reduce CaOx crystal adhesion to renal epithelial HK-2 cells. Our findings highlighted the important role of N-1 in reducing renal CaOx crystals by regulating arginine metabolism in the gut microbiota. Probiotics containing L. plantarum N-1 may be potential therapies for preventing renal CaOx stones.

scholarly journals Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4874
Author(s):  
Samina Rubnawaz ◽  
Nosheen Akhtar ◽  
Rashid Mahmood ◽  
Asif Khan ◽  
Mohammad K. Okla ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Reducing Power ◽  
Rol Genes ◽  
Hydroxyl Ion ◽  
Total Antioxidant ◽  
Endangered Medicinal Herb

Ajuga bracteosa Wall. ex Benth. is an endangered medicinal herb traditionally used against different ailments. The present study aimed to create new insight into the fundamental mechanisms of genetic transformation and the biological activities of this plant. We transformed the A. bracteosa plant with rol genes of Agrobacterium rhizogenes and raised the regenerants from the hairy roots. These transgenic regenerants were screened for in vitro antioxidant activities, a range of in vivo assays, elemental analysis, polyphenol content, and different phytochemicals found through HPLC. Among 18 polyphenolic standards, kaempferol was most abundant in all transgenic lines. Furthermore, transgenic line 3 (ABRL3) showed maximum phenolics and flavonoids content among all tested plant extracts. ABRL3 also demonstrated the highest total antioxidant capacity (8.16 ± 1 μg AAE/mg), total reducing power, (6.60 ± 1.17 μg AAE/mg), DPPH activity (IC50 = 59.5 ± 0.8 μg/mL), hydroxyl ion scavenging (IC50 = 122.5 ± 0.90 μg/mL), and iron-chelating power (IC50 = 154.8 ± 2 μg/mL). Moreover, transformed plant extracts produced significant analgesic, anti-inflammatory, anticoagulant, and antidepressant activities in BALB/c mice models. In conclusion, transgenic regenerants of A. bracteosa pose better antioxidant and pharmacological properties under the effect of rol genes as compared to wild-type plants.

scholarly journals The increase of blood creatinine levels and the gastric histopathology of rat after feeding of porang (Amorphophallus oncophyllus) flour treated with strobilantehes crispa

2019 ◽  
Vol 6 (3) ◽  
pp. 113
Author(s):  
Ernawati Ernawati ◽  
Veriani Aprilia ◽  
Retno Pangastuti
Keyword(s):  
Acute Toxicity ◽  
Calcium Oxalate ◽  
Rattus Norvegicus ◽  
Toxicity Test ◽  
Food Additives ◽  
Test Group ◽  
Control Group ◽  
Acute Toxicity Test ◽  
Blood Creatinine

<p align="center"><strong>ABSTRAK</strong></p><p>Latar belakang: Umbi Porang (Amorphophallus oncophyllus) meru[akan umbi asli Indonesia yang mengandung glucomannan. Glukomanan digunakan sebagai bahan tambahan makanan dan suplemen makanan bagi penderita diabetes, tekanan darah tinggi, konstipasi dan penurunan berat badan. Namun, umbi porang mengandung kalsium oksalat yang menyebabkan gatal jika dikonsumsi oleh karena itu kesiapan persiapan yang tepat. Perendaman dari S. crispa in vivo  elah terbukti menurunkan kadar kalsium oksalat. Namun, kelebihan konsumsi kalsium oksalat dapat menyebabkan gangguan fungsi ginjal, terutama pada laju flomerasi glomerulus (GFR) dan mempengaruhi kerja ginjal, yaitu penyerapan dan kreatinin fltrasi.</p><p>Tujuan: Untuk mengetahui pengaruh porang orang dengan S. crispa (keji beling) terhadap kreatinin darah dan histopatologi lambung tikus (Rattus norvegicus) Wistar pada uji toksisitas akut.<em><br /> </em>Metode: Penelitian ini menggunakan experimental with one test group, without control group. Subjek 20 tikus Wistar betina putih (Rattus norvegicus) dengan berat 110-180 gram, usia 8 - 12 minggu, sehat, dan normal. Kelompok tikus dibagi menjadi Tepung Porang Murni (TPM) dan Tepung Porang dengan ekstrak etanol S. crispa (TPK). Setiap kelompok menggunakan dosis 2000 mg / kg berat badan (BW) dan 5000 mg / kgBB. Data kadar kreatinin dikumpulkan pada 24 dan 72 jam setelah makan juga dikonfirmasikan oleh tikus lambung. Analisis data menggunakan One-Way analysis of ariance (ANOVA).</p><p>Hasil: Jenis larutan dan frekuensi perendaman tidak berpengaruh pada kadar kalsium oksalat padatepung porang, sedangkan lama perendaman tidak memberi efek. Kadar kreatinin darah tikus meningkat setelah pemberian pakan Tepung Porang  urni (TPM) dan Tepung Porang  dengan Ekstraksi Keji Beling (TPK) dengan dosis 5000 mg/kgBB.</p><p>Kesimpulan: Pemberian tepung porang dengan perlakuan ekstrak S. crispa aman sampai dosis 5000 mg/kgBW. Ini terbukti dengan normalitas waktu di tingkat kadar kreatinin.</p><p><strong><em><br /> </em>KATA KUNCI:</strong> porang umbi, kalsium oksalat, rongga, kreatinin, uji toksisitas akut.<strong></strong></p><p align="center"><strong> </strong></p><p align="center"><strong>ABSTRACT</strong></p><p><strong><em>Background: </em></strong><em>Porang (Amorphophallus oncophyllus) tuber is the original Indonesian tuber containing glucomannan. Glucomannan is utilized as food additives and food supplements for people who have problems with diabetes, high blood pressure, constipation and weight loss. However, it contains calcium oxalate which causes itchy if it is consumed therefore itness appropriate preparation. Soaking of S. crispa in vivo has proven lowering the levels of calcium oxalate. However, the excess consumption of oxalate calcium can cause renal function disorders, especially at the </em><em>glomerular filtration rate (GFR)</em><em> </em><em>and affects the kidneys work, ie absorption and filtration creatinine.</em></p><p><strong><em>Objectives:</em></strong><em> The objective of this study is to know the influence of the porang flour with S. crispa (keji beling) on the level of blood creatinine and gastric histopathology of rats (Rattus norvegicus) Wistar on acute toxicity test.</em></p><p><strong><em>Methods:</em></strong><em> This research used experimental with one test group, without control group design. The subjects were 20 white female Wistar rats (Rattus norvegicus) with the weight of 110-180 grams, ages of 8 - 12 weeks, healthy, and normal. Rats were divided into. Native porang flour (TPM) and porang flour treated with ethanol extracts of S. crispa (TPK) groups. Each of groups used dosage  of 2000 mg / kg body weight (BW) and 5000 mg/kgBW. Data of creatininee levels were collected at 24<sup>th</sup> and 72<sup>nd</sup> hours after feeding was also confirmed by gastric rats. The data analysis was </em><em>used One-Way analysis  of variance (ANOVA) .</em><em></em></p><p><strong><em>Result</em></strong><strong><em>s:</em></strong><em> The type of solution and the frequency of soaking had no effect on calcium oxalate levels in the Porang, whereas the length of soaking is not giving an effect. Blood levels of creatinine rats enhanced after feeding of TPM and TPK. At the dosage of 5000 mg/kgBW.</em></p><p><strong><em>Conclusions: </em></strong>The feeding of porang flour treated with <em>S. crispa  </em>was safe until the dosage of 5000 mg/kgBW. It was proven by time normality in creatinine levels.</p><p> </p><p><strong>KEYWORDS:</strong><em> porang tuber, calcium oxalate, cavity, creatinine, acute toxicity test.</em></p>

scholarly journals Outcomes of Gallic Acid on Alternariol Induced Cyto-Morphic and Genotoxic In Vivo Changes in Parotid Gland: 4-HNE Incorporated

Biomedicines ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 84
Author(s):  
Mai A. Samak ◽  
Ahmed Elshatory ◽  
Eman M. Mohamed
Keyword(s):  
Dna Damage ◽  
Parotid Gland ◽  
Gallic Acid ◽  
Healthy Adult ◽  
Control Group ◽  
Immunohistochemical Expression ◽  
Healthy Adult Male ◽  
Tissue Homogenates ◽  
Acid Administration

Alternaria toxins are emerging mycotoxins that gained considerable interest with increasing evidence of their existence and toxicological properties. There is limited research and insufficient data about their in vivo hazardous effects. We designed this study to evaluate histopathological and genotoxic in vivo impacts of alternariol (AOH) on the parotid gland as well as to assess the competency of gallic acid (GA) in reversing these effects. Forty healthy adult male Wister rats were utilized and assigned equally on control, GA, alternariol and AOH+ gallic treated groups. Parotid gland samples from experimental groups were collected and then examined for histopathological, ultrastructural and immunohistochemical examination for 4-hydroxynonenal “4-HNE as lipid peroxidation marker” as well as Comet assay for DNA damage. Additionally, parotid tissue homogenates were tested for catalase “CAT”, superoxide dismutase “SOD” and malondialdehyde “MDA” levels. Our data proved that alternariol produced various histopathological and ultrastructural alterations of parotid acini as well as significant DNA damage, significant reduction of CAT and SOD enzymatic activity and significant boosting of 4-HNE immunohistochemical expression and MDA levels as compared to control group. On the other hand, gallic acid administration almost restored histological and ultrastructural parotid architecture, 4-HNE immune-expression and biochemical levels. Ultimately, we demonstrated alternariol-induced histopathological and genotoxic alterations on parotid gland as well as the competency of gallic acid in reversing these effects.

scholarly journals In vitro antioxidant and antinociceptive potentialities of methanolic extract of Litsea glutinosa

2013 ◽  
Vol 47 (4) ◽  
pp. 401-406 ◽  
Author(s):  
NN Rumzhum ◽  
MM Rahman ◽  
AA Sharukh ◽  
SA Chowdhury ◽  
MN Pervin
Keyword(s):  
Methanolic Extract ◽  
Antinociceptive Effect ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Scavenging Activity ◽  
Total Antioxidant ◽  
Litsea Glutinosa

The methanolic extract of Litsea glutinosa (Lauraceae) was evaluated for in vitro antioxidant activity by determination of hydrogen peroxide scavenging activity, total antioxidant capacity, assay of nitric oxide scavenging activity and reducing power test and in vivo antinociceptive effect in acetic acid induced writhing model in swiss albino mice. The results revealed the presence of pronounced antioxidant property as compared with ascorbic acid used as standard and a dose-dependent (250 and 500 mg/kg) analgesic effect. The antioxidant and antinociceptive properties observed seem to be in good accordance with the traditional uses of Litsea glutinosa. Bangladesh J. Sci. Ind. Res. 47(4), 401-406, 2012 DOI: http://dx.doi.org/10.3329/bjsir.v47i4.14069

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