scholarly journals Synthesis, Characterization and Assessment of the Antioxidant Activity of Cu(II), Zn(II) and Cd(II) Complexes Derived from Scorpionate Ligands

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5298
Author(s):  
Aurel Tăbăcaru ◽  
Rais Ahmad Khan ◽  
Giulio Lupidi ◽  
Claudio Pettinari
Keyword(s):  
Antioxidant Activity ◽  
Metal Complexes ◽  
Specific Activity ◽  
Antioxidant Properties ◽  
Proton Nuclear Magnetic Resonance ◽  
Partial Hydrolysis ◽  
Enzymatic Assays ◽  
New Family ◽  
Ic50 Values ◽  
Scorpionate Ligands

Seeking to enrich the yet less explored field of scorpionate complexes bearing antioxidant properties, we, here, report on the synthesis, characterization and assessment of the antioxidant activity of new complexes derived from three scorpionate ligands. The interaction between the scorpionate ligands thallium(I) hydrotris(5-methyl-indazolyl)borate (TlTp4Bo,5Me), thallium(I) hydrotris(4,5-dihydro-2H-benzo[g]indazolyl)borate (TlTpa) and potassium hydrotris(3-tert-butyl- pyrazolyl)borate (KTptBu), and metal(II) chlorides, in dichloromethane at room temperature, produced a new family of complexes having the stoichiometric formula [M(Tp4Bo,5Me)2] (M = Cu, 1; Zn, 4; Cd, 7), [M(Tpa)2] (M = Cu, 2; Zn, 5; Cd, 8), [Cu(HpztBu)3Cl2] (3), [Zn(TptBu)Cl] (6) and [Cd(BptBu)(HpztBu)Cl] (9). The obtained metal complexes were characterized by Fourier transform infrared spectroscopy, proton nuclear magnetic resonance and elemental analysis, highlighting the total and partial hydrolysis of the scorpionate ligand TptBu during the synthesis of the Cu(II) complex 3 and the Cd(II) complex 9, respectively. An assessment of the antioxidant activity of the obtained metal complexes was performed through both enzymatic and non-enzymatic assays against 1,1-diphenyl-2-picryl- hydrazyl (DPPH·), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+·), hydroxyl (HO·), nitric oxide (NO·), superoxide (O2−) and peroxide (OOH·) radicals. In particular, the complex [Cu(Tpa)2]⋅0.5H2O (2) exhibited significant antioxidant activity, as good and specific activity against superoxide (O2−·), (IC50 values equal to 5.6 ± 0.2 μM) and might be identified as auspicious SOD-mimics (SOD = superoxide dismutase).

scholarly journals AMINO ACID COMPOSITION AND ANTIOXIDANT PROPERTIES OF FIVE EDIBLE MUSHROOM SPECIES FROM KASTAMONU, TURKEY

2018 ◽  
Vol 15 (2) ◽  
pp. 80
Author(s):  
Temel kan Bakır ◽  
Mansor Boufars ◽  
Mertcan Karadeniz ◽  
Sabri Ünal
Keyword(s):  
Antioxidant Activity ◽  
Amino Acid ◽  
Free Amino ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Edible Mushroom ◽  
Total Phenolic ◽  
Mushroom Species ◽  
Boletus Edulis ◽  
Ic50 Values

Background: Hydnum repandum, Cantharellus cibarius, Ramaria fennica, Boletus edulis, and Craterellus cornucopioides are all wild edible mushroom species from the Kastamonu province. The aim of this study was to investigate antioxidant properties and amino acid contents of these mushrooms. Materials and Methods: The mushrooms were analyzed for their free amino acid compositions using a high performance Amino Acid analyzer (Biochrom 30). Also, antioxidant activity and total phenolic concentrations of five different mushroom species were studied by using spectrophotometric methods. Results: The mushrooms contained 17 amino acids (g/100 g): Glutamic acid (2.56–1.11), Alanine (1.49–0.54), Arginine (1.62–6.77), Aspartic acid (1.45–0.81), Leucine (1.08–0.64), Methionine (1.05–0.06), Valine (1.05–0.66), Lysine (1.01–0.57), Serine (0.68–0.38), Cysteine (0.88–0.11), Isoleucine (0.61–0.39), Glycine (0.82–0.35), Threonine (0.82–0.44), Phenylalanine (0.66–0.42), Proline (0.60–0.47), Tyrosine (0.58–0.38), and Histidine (0.48–0.22). The total free amino acid (TAA) contents ranged from 169.2 mg/g in Boletus edulis to 89.1 mg/g in Hydnum repandum. These five different mushroom species contain eight essential amino acid species except tryptophan. The antioxidant activity of mushroom extracts was expressed as the percentage of DPPH radical inhibition and IC50 values (mg/mL). The percentage of inhibition ranged from 2.38% to 88.05% and IC50 values ranged from 0.03 to 13.98 mg/mL. The total phenolic content ranged from 0.66 to 7.74 mg/g of dry mushroom extract, expressed as gallic acid equivalents. Conclusion: Methanolic extract of Boletus edulis showed the highest phenolic content and strong antioxidant activity. As a result, the significant linear correlation between the values for the total phenolic content and antioxidant activity of mushroom extracts was corroborated in all of the investigated mushroom species, with the exception of Craterellus cornucopioides.

scholarly journals Bioprospection of Enggano Macroscopic Fungi as Antibacterial and Antioxidant Agents

2021 ◽  
Vol 20 (2) ◽  
pp. 201-210
Author(s):  
Evana Evana ◽  
Kartika Dyah Palupi ◽  
Listiana Oktavia ◽  
Ahmad Fathoni
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Properties ◽  
Antioxidant Compounds ◽  
Activity Assay ◽  
Tropical Country ◽  
Antioxidant Agents ◽  
Ic50 Values ◽  
Weak Activity ◽  
Antibacterial And Antioxidant Activity

Macrofungi in Indonesia have not been widely studied for their pharmacological activity, especially as a source of antibacterial and antioxidant properties, even though Indonesia as a tropical country has quite a high diversity of macrofungi. This study aims to reveal the potential of macrofungi from the Enggano forest as a source of antibacterial and antioxidant compounds. Four types of macrofungi were collected and their metabolites were extracted using four types of organic solvents. Antibacterial and antioxidant activity assay of the extract was carried out using the TLC Bioautography method. From the sixteen macrofungal extracts, there is one extract that has the strongest antibacterial activity compared to the others, namely n-hexane Coriolopsis polyzona. It showed moderate antibacterial activity against Staphylococcus aureus and Escherichia coli with MIC values of 256–128 μg/mL, respectively. Meanwhile, the antioxidant activity of the macrofungal extracts showed weak activity with IC50 values of 3080–7370 μg/mL (AAI values of 0.033–0.079).

scholarly journals Antioxidant Activity and Cytotoxicity against Cancer Cell Lines of the Extracts from Novel Xylaria Species Associated with Termite Nests and LC-MS Analysis

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1557
Author(s):  
Niwana Wangsawat ◽  
Lutfun Nahar ◽  
Satyajit D. Sarker ◽  
Cherdchai Phosri ◽  
Andrew R. Evans ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Specific Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Bioactive Metabolites ◽  
Total Phenolic ◽  
Ic50 Values

Xylaria species associated with termite nests or soil have been considered rare species in nature and the few which have been reported upon have been found to act as a rich source of bioactive metabolites. This study evaluated 10 ethyl acetate extracts of five new Xylaria species associated with termite nests or soil for their antioxidant activity, and cytotoxicity against different cancer and normal cell lines. DPPH and ABTS radical scavenging activities of the extracts demonstrated strong capacity with low IC50 values. The highest observed activities belonged to X. vinacea SWUF18-2.3 having IC50 values of 0.194 ± 0.031 mg/mL for DPPH assay and 0.020 ± 0.004 mg/mL for ABTS assay. Total phenolic content ranged from 0.826 ± 0.123 to 3.629 ± 0.381 g GAE/g crude extract which correlated with antioxidant activities. The high total phenolic content could contribute to the high antioxidant activities. Cytotoxicity was recorded against A549, HepG2, HeLa and PNT2 and resulted in broad spectrum to specific activity depending on the cell lines. The highest activities were observed with X. subintraflava SWUF16-11.1 which resulted in 11.15 ± 0.32 to 13.17 ± 2.37% cell viability at a concentration of 100 µg/mL. Moreover, LC-MS fingerprints indicated over 61 peaks from all isolates. There were 18 identified and 43 unidentified compounds compared to mass databases. The identified compounds were from various groups of diterpenoids, diterpenes, cytochalasin, flavones, flavonoids, polyphenols, steroids and derivatives, triterpenoids and tropones. These results indicate that Xylaria spp. has abundant secondary metabolites that could be further explored for their therapeutic properties.

scholarly journals Habitat Affects the Chemical Profile, Allelopathy, and Antioxidant Properties of Essential Oils and Phenolic Enriched Extracts of the Invasive Plant Heliotropium Curassavicum

Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 482 ◽  
Author(s):  
Ahmed M. Abd-ElGawad ◽  
Abdelsamed I. Elshamy ◽  
Saud L. Al-Rowaily ◽  
Yasser A. El-Amier
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Essential Oils ◽  
Invasive Plant ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Bioactive Constituents ◽  
Chenopodium Murale ◽  
Two Samples ◽  
Ic50 Values

The variation in habitat has a direct effect on the plants and as a consequence, changes their content of the bioactive constituents and biological activities. The present study aimed to explore the variation in the essential oils (EOs) and phenolics of Heliotropium curassavicum collected from the coastal and inland habitats. Additionally, we determined their antioxidant and allelopathic activity against the weed, Chenopodium murale. Fifty-six compounds were identified as overall from EOs, from which 25 components were identified from the coastal sample, and 52 from the inland one. Sesquiterpenes were the main class in both samples (81.67% and 79.28%), while mono (3.99% and 7.21%) and diterpenes (2.9% and 1.77%) represented minors, respectively. Hexahydrofarnesyl acetone, (-)-caryophyllene oxide, farnesyl acetone, humulene oxide, farnesyl acetone C, and nerolidol epoxy acetate were identified as major compounds. The HPLC analysis of MeOH extracts of the two samples showed that chlorogenic acid, rutin, and propyl gallate are major compounds in the coastal sample, while vanilin, quercetin, and 4′,7-dihydroxyisoflavone are majors in the inland one. The EOs showed considerable phytotoxicity against C. murale with IC50 value of 2.66, 0.59, and 0.70 mg mL−1 for germination, root, and shoot growth, respectively from the inland sample. While the coastal sample attained the IC50 values of 1.58, 0.45, and 0.66 mg mL−1. MeOH extracts revealed stronger antioxidant activity compared to the EOs. Based on IC50 values, the ascorbic acid revealed 3-fold of the antioxidant compared to the EO of the coastal sample and 4-fold regarding the inland sample. However, the ascorbic acid showed 3-fold of the antioxidant activity of the MeOH extracts of coastal and inland samples. Although H. curassavicum is considered as a noxious, invasive plant, the present study revealed that EO and MeOH extracts of the H. curassavicum could be considered as promising, eco-friendly, natural resources for antioxidants as well as weed control, particularly against the weed, C. murale.

scholarly journals Total Phenolic Contents and Antioxidant Properties of Algerian Arbutus unedo L. Extracts

2020 ◽  
Vol 10 (3-s) ◽  
pp. 159-168
Author(s):  
Saliha Laouicha ◽  
Abderrahmane Senator ◽  
Abdallah Kherbache ◽  
Hamama Bouriche
Keyword(s):  
Antioxidant Activity ◽  
Folk Medicine ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Arbutus Unedo ◽  
Phenolic Contents ◽  
Antioxidant Agents ◽  
Ic50 Values

Arbutus unedo L. is a plant widely distributed in the Mediterranean basin and North Africa, frequently used in traditional folk medicine to treat diabetes and arterial hypertension. This study aims to evaluate the phenolic composition and antioxidant activity of ethyl acetate (EA.E) and n-butanolic (But.E) extracts prepared from leaves of Arbutus unedo. Total phenolic and flavonoids contents were determined spectrophotometrically. The antioxidant activity was evaluated using DPPH•, OH•, H2O2, O•-2, ß-carotene bleaching and AAPH-induced erythrocyte oxidative hemolysis assays. The phytochemical analysis showed the presence of polyphenols and flavonoids in both extracts. The high amount was observed in EA.E which exerted the stronger antioxidant effect, with IC50 values of 3.43 μg/mL, 323.45 µg/mL, 38.40 µg/mL and 11.56 µg/mL, in DPPH•, OH•, O•-2 and H2O2 tests, respectively. Both extracts inhibited β-carotene bleaching, but EA.E is always more potent (92%) than But.E (85%). Furthermore, the EA.E showed the highest protective effect on erythrocyte hemolysis induced by AAPH, with half time hemolysis (HT50) of 122.02 min at 40 µg/mL. Taken together, this study showed that Arbutus unedo leaf extracts possess strong antioxidant potential, which may be attributed to the presence of a high amount of polyphenolic constituents. So, this plant might be exploited as a potential source of natural antioxidant agents for pharmaceutical and food applications. Keywords: oxidative stress, antioxidant, phenolic compounds, Arbutus unedo.

scholarly journals Phytochemical Profile and Antioxidant Activity of Nigella sativa L Growing in Morocco

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Salima Tiji ◽  
Ouijdane Benayad ◽  
Mohamed Berrabah ◽  
Ibrahim El Mounsi ◽  
Mostafa Mimouni
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Nigella Sativa ◽  
Antioxidant Properties ◽  
Acetone Extract ◽  
Radical Scavenger ◽  
Negative Effect ◽  
Ic50 Values ◽  
Positive Effect ◽  
Β Carotene

Background. Nigella sativa L (NS) is a powerful antioxidant and medicinal plant with many therapeutic applications particularly in traditional medicine for respiratory, gastrointestinal, rheumatic, and inflammatory disorders, as well as cancer. Objective. The aim of this study is to extract the active ingredients from the Moroccan Nigella sativa L and determine its antioxidant properties. We hypothesize that the separation of the compounds from Nigella sativa L has either a positive or negative effect on antioxidants. To study this, we explored different methods to simultaneously extract and separate compounds from Nigella sativa L and performed antioxidant tests (β-carotene and DPPH) for all collected fractions. Methods. Nigella sativa L was hot-extracted by Soxhlet and mother extracts and was separated using silica column chromatography with adequate eluents. Qualitative phytochemical tests to determine the chemical families in Nigella sativa L seeds were performed on the fractions. They were also identified and characterized by GC-MS and HPLC-DAD. Then, antioxidant activity was examined by β-carotene bleaching and DPPH radical scavenger tests. Results and Conclusion. The mother extract hexane FH generated eight different fractions (SH1-8) and the acetone extract FA generated 11 fractions (SA1-11). The FH fractions had a high percentage of fatty acids, and the FA fractions had some interesting polyphenols derivative compounds. Phytochemical screening revealed secondary metabolites such as polyphenols flavonoids, alkaloids, steroids, terpenes coumarins, tannins, and saponins. We found that only two solvents (hexane, acetone) of different polarities could easily extract and simultaneously separate the components of Nigella sativa L. The antioxidant fractions that we collected had close activity to reference compounds but were more active than the corresponding mother extracts. Moreover, several IC50 values of fractions from acetone extract were better than those from hexane. Therefore, the antioxidant activity of Nigella sativa L is more attributed to flavonoids and polyphenols than fatty acids. In summary, the separation of hexane extract presents a more pronounced positive effect for antioxidant tests than acetone extract.

scholarly journals In vitro antioxidant properties of 2-(4-(2-hydroxybenzylideneamino)benzylideneamino)benzoic acid

2021 ◽  
Vol 5 (3) ◽  
pp. 7-12
Author(s):  
Zehra Kübra YILMAZ ◽  
Belma ASIM ◽  
Ozlem OZDEMIR
Keyword(s):  
Antioxidant Activity ◽  
Schiff Base ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ferrous Ion ◽  
Synthetic Antioxidants ◽  
Scavenging Capacity ◽  
Total Antioxidant ◽  
Ic50 Values

In recent years, it has attracted great attention to determine and appraise synthetic antioxidants with high free radical scavenging capacity associated with various disorders. Because synthetic antioxidants are widely used in place of native antioxidants as they are influential and less expensive. In this study, the antioxidant activity of the newly synthesized asymmetric diimine Schiff base was determined by using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging, ferrous ion chelation (FIC) activity and total antioxidant activity (Phosphomolybdenum assay) methods. The effective concentration (IC50) values were calculated for the Schiff base and standards. According to the results of DPPH radical scavenging, ferrous ion chelation and total antioxidant methods, Schiff base showed a well antioxidant activity. It was determined that Schiff base is reactive towards DPPH radicals (IC50, 180.0 μM) and especially Fe+2 ions (IC50, 76.3 μM). It was also found that it actively reduces the Mo(VI) ion to the Mo(V) form (IC50, 121.5 μM). The our results indicate Schiff base, may be a fine candidate as a source of synthetic antioxidants and possibly strong drug.

scholarly journals New Nanomaterials with Intrinsic Antioxidant Activity by Surface Functionalization of Niosomes with Natural Phenolic Acids

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 766
Author(s):  
Elisabetta Mazzotta ◽  
Carla Orlando ◽  
Rita Muzzalupo
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Surface Functionalization ◽  
Antioxidant Properties ◽  
Tween 80 ◽  
Smart Devices ◽  
New Family ◽  
Improved Stability ◽  
Evaporation Technique

Nanoantioxidants have emerged as smart devices able to provide improved stability and biocompatibility and sustained and targeted release of conventional antioxidants. In the current research, a new family of nanoantioxidants has been developed by covalently grafting gallic (GA), caffeic (CF) and ferulic (FR) acid on the surfaces of Tween 80 niosomes. First, empty and curcumin (CUR)-loaded vesicles were prepared using a thin-layer evaporation technique and then functionalized with phenolic acids using carbodiimide chemistry. Nanoantioxidants obtained were characterized in terms of size, polydispersity index, zeta potential, and loading efficiency. Their antioxidant activity was studied by ABTS and DPPH assays. Surface functionalization of empty and CUR-loaded vesicles provided stable vesicles with intrinsic antioxidant properties. In vitro antioxidant assays highlighted that vesicles functionalized with FR or GA exhibited better antioxidant activity compared to CF-grafted niosomes. Furthermore, vesicles loaded with CUR and functionalized with GA and CF showed an enhanced scavenging ability of ABTS and DPPH radicals, compared to the single antioxidant-loaded formulations, highlighting an important synergic effect of CUR when used in combination with GA ad CF.

Antioxidant activity of Gryllus assimilis meal hydrolysed by Aspergillus oryzae proteases

2021 ◽  
pp. 1-4
Author(s):  
J. Machado ◽  
J.C. Porfírio ◽  
M.A. Koike ◽  
L.F. Fleuri ◽  
M.P. Bagagli
Keyword(s):  
Antioxidant Activity ◽  
Aspergillus Oryzae ◽  
Nutritional Value ◽  
Animal Feed ◽  
Antioxidant Properties ◽  
Partial Hydrolysis ◽  
Enzymatic Extract ◽  
Alternative Protein ◽  
Hydrolysis Of ◽  
Initial Results

The consumption of insects is an alternative protein source of a high nutritional value, in comparison to other traditional animal and vegetable proteins, and is suitable for food and as animal feed. This work aimed to study the antioxidant activity of cricket meal (Gryllus assimilis) partially hydrolysed by proteases from a Aspergillus oryzae, which was cultivated in the cricket meal. The cricket meal showed great potential for obtaining protease from A. oryzae, with an average enzymatic activity of 112±8 U/g of dry substrate after 96 h of fermentation. The enzymatic extract applied to the cricket meal increased its antioxidant properties, increasing the reduction of the 2,2-diphenyl-1-picrylhydrazyl radical by 2 times, compared to non-hydrolysed cricket meal. These initial results are promising, demonstrating the benefits of the partial hydrolysis of cricket meal.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN BUAS-BUAS (Premna serratifolia L.) ASAL KABUPATEN MELAWI PROVINSI KALIMANTAN BARAT DENGAN METODE DPPH

2020 ◽  
Vol 3 (2) ◽  
pp. 405-412
Author(s):  
Weni Puspita ◽  
◽  
Dina Yuspita Sari ◽  
Ika Ristia Rahman ◽  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Cell Damage ◽  
Antioxidant Properties ◽  
Free Radical Oxidation ◽  
The Body ◽  
Radical Oxidation ◽  
Ic50 Values ◽  
Ethanol Extracts

Buas (Premna serratifolia L.) contains secondary metabolites of flavonoids, saponins, tannins and triterpenoids/steroids, where flavonoids are known to have antioxidant properties and counteract free radicals. Antioxidants are compounds that can fight toxic hazards and reduce cell damage to the body caused by free radical oxidation. The purpose of this study was to determine the antioxidant activity of ethanol extracts of buas-buas leaves was carried out by DPPH (2,2-diphenyl-1-picrylhydrazyl), using UV-Vis spectrophotometry at wavelength 518.40 nm with various concentrations of 10 ppm, 20 ppm, 30 ppm, 40 ppm and 50 ppm. The results showed that Buas Buas leaves (Premna serratifolia L.) had an antioxidant activity with IC50 values ??of 20.66 ug / mL.

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