scholarly journals Phytochemical and In-Vivo Anti-Arthritic Significance of Aloe thraskii Baker in Combined Therapy with Methotrexate in Adjuvant-Induced Arthritis in Rats

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3660
Author(s):  
Rania M. Kamal ◽  
Manal M. Sabry ◽  
Zeinab Y. Aly ◽  
Mohamed S. Hifnawy
Keyword(s):  
Rheumatoid Arthritis ◽  
Liver Function ◽  
Liver Toxicity ◽  
Inflammatory Marker ◽  
Combined Therapy ◽  
Ethanolic Extract ◽  
Serum Levels ◽  
Chemical Characters ◽  
Stress Mediators

Unlike other widely known Aloe species used for treatment of rheumatoid arthritis, this species suffers from a lack of sufficient studies on its biological and chemical characters. This is what drove us to perform this work to evaluate the in vivo anti-arthritic potential of its leaf ethanolic extract. The in vivo anti-arthritic activity of the leaf ethanolic extract at 100 and 200 mg/kg/day b.wt. was evaluated alone and in combination with methotrexate (MTX) using complete Freund’s adjuvant. Serum levels of rheumatoid factor, anti-cyclic citrullinated peptide (anti-CCP), cytokines pro-inflammatory marker, inflammatory mediator serum levels, and oxidative stress mediators were analyzed, in addition to liver function. Orientin, isoorientin, β-sitosterol, its palmitate and its glucoside were isolated. The combined therapy of MTX and the leaf ethanolic extract (especially at 200 mg/kg b.wt.) group showed better activity compared to MTX alone. Moreover, the combined therapy provided additional benefits in lowering the liver toxicity by comparison to MTX alone. We concluded that a synergetic combination of the leaf ethanolic extract and MTX is beneficial in the management of rheumatoid arthritis with fewer side effects on liver function, as well as the possibility of the leaf extract to stand alone as an effective natural anti-arthritic agent.

scholarly journals Hepatoprotective Actions of Ascorbic Acid, Alpha Lipoic Acid and Silymarin or Their Combination Against Acetaminophen-Induced Hepatotoxicity in Rats

Medicina ◽  
2019 ◽  
Vol 55 (5) ◽  
pp. 181 ◽  
Author(s):  
Anmar M. Abdulrazzaq ◽  
Mujtaba Badr ◽  
Omar Gammoh ◽  
Asad A. Abu Khalil ◽  
Bayan Y. Ghanim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Liver Function ◽  
Lipoic Acid ◽  
Rat Hepatocytes ◽  
Serum Levels ◽  
Liver Function Tests ◽  
Alpha Lipoic Acid

Background and objectives: Ascorbic acid, alpha lipoic acid (ALA) and silymarin are well-known antioxidants that have hepatoprotective effects. This study aims to investigate the effects of these three compounds combined with attenuating drug-induced oxidative stress and cellular damage, taking acetaminophen (APAP)-induced toxicity in rats as a model both in vivo and in vitro. Materials and Methods: Freshly cultured primary rat hepatocytes were treated with ascorbic acid, ALA, silymarin and their combination, both with and without the addition of APAP to evaluate their in vitro impact on cell proliferation and mitochondrial activity. In vivo study was performed on rats supplemented with the test compounds or their combination for one week followed by two toxic doses of APAP. Results: Selected liver function tests and oxidative stress markers including superoxide dismutase (SOD), malondialdehyde (MDA) and oxidized glutathione (GSSG) were detected. The in vivo results showed that all three pretreatment compounds and their combination prevented elevation of SOD and GSSG serum levels indicating a diminished burden of oxidative stress. Moreover, ascorbic acid, ALA and silymarin in combination reduced serum levels of liver enzymes; however, silymarin markedly maintained levels of all parameters to normal ranges. Silymarin either alone or combined with ascorbic acid and ALA protected cultured rat hepatocytes and increased cellular metabolic activity. The subjected agents were capable of significantly inhibiting the presence of oxidative stress induced by APAP toxicity and the best result for protection was seen with the use of silymarin. Conclusions: The measured liver function tests may suggest an augmented hepatoprotection of the combination preparation than when compared individually.

Evaluation of Hepatoprotective activity of Ethanolic Extract of Garuga pinnata Roxburgh leaves against Carbon tetrachloride induced Hepatotoxicity in rats.

2021 ◽  
pp. 2375-2380
Author(s):  
Sandeep Chavan ◽  
Remeth Dias ◽  
Chandrakant Magdum
Keyword(s):  
Carbon Tetrachloride ◽  
Biochemical Parameters ◽  
Histopathological Examination ◽  
Liquid Paraffin ◽  
Ethanolic Extract ◽  
Serum Levels ◽  
Hepatoprotective Activity ◽  
Antioxidant Defense Enzymes ◽  
Serum Glutamate

In this study we investigated the in vivo Hepatoprotective activity of ethanolic extract of Garuga pinnata (EEGP) leaves in Carbon tetrachloride (CCl4) induced hepatotoxicity using wistar rats of either sex as model. Hepatotoxicity was induced by the administration of CCl4 intraperitoneally (0.125ml CCl4 in liquid paraffin (1:1) per 100g body weight). Garuga pinnata leaves extract at different dose levels (200 and 400mg/kg, p.o.) showed the dose dependant hepatoprotective effect and was compared with well known standard hepatoprotective Silymarain (100mg/kg). When groups were treated with CCl4, significant increase in serum biochemical parameters such as Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Glutamate Pyruvate Transaminase (SGPT), Alkaline phosphate (ALP), Acid Phosphate (ACP), Creatinine and alteration of tissue biochemical parameters such as reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (LPO), and the total proteins were observed. The histopathological examination of the CCl4 treated groups showed sinusoidal congestion, centrilobular necrosis, marked vacuolations and congestion. However, pretreatment with extract of leaves of Garuga pinnata significantly reduced the increased serum levels of biochemical parameters and restored antioxidant defense enzymes level to its normal. Moreover, histopathology of leaves extract treated groups showed normal architecture with minimal sinusoidal congestion. Taken together, our study concludes that EEGP to be a more potential agent for caring liver from CCl4 induced damage.

Antioxidant potentials of Terminalia catappa leaf extractin Streptozotocin induced diabetes in rats

2016 ◽  
Author(s):  
Natarajan Divya ◽  
Arumugam Vijaya Anand
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Ethanolic Extract ◽  
Serum Levels ◽  
Albino Rats ◽  
Low Density ◽  
Subsequent Increase ◽  
Terminalia Catappa ◽  
Percolation Method

The objective of this study to evaluate the antioxidant capacity of the ethanolic extract of Terminalia catappa in vivo in Wistar albino rats. Streptozotocin (STZ) used as toxin it induces diabetes, damages the cell membrane and causes oxidative stress. The leaves (T. catappa) were extracted using 95 % ethanol by hot continuous percolation method. The extracts were concentrated by rotaevaporador. The residues extract were administrated orally to the STZ induced diabetes animals. After 45th day the animals were sacrificed and blood, liver tissues were collected and various antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), were reduced in the STZ alone treated animals with subsequent increase in LPO. The serum levels of total cholesterol (TC), triglycerides (TG), free fatty acids (FFA), phospholipids, low density lipoprotein (LDL), very low density lipoprotein (VLDL), and high density lipoprotein (HDL) was found out. Treatment with ethanolic extract of T. catappa at a dose of 300, 500 mg/kg once in a day has altered the levels of biochemical markers and brings back to near normal levels. Among which the dose 500 mg/kg having scavenging action to eradicate free radicals and maintained antioxidant status. The statistical data of P Values less than 0.001 were considered as level of significance.

scholarly journals Isoniazid induced hepatotoxicity and its amelioration with ethanolic extract of stem bark of Berberis lycium Royale in mice

2017 ◽  
Vol 6 (8) ◽  
pp. 1865 ◽  
Author(s):  
Saima Rafiq ◽  
Khalida Ajmal ◽  
Ayesha Afzal
Keyword(s):  
Elevated Serum ◽  
Ethanolic Extract ◽  
Serum Levels ◽  
Stem Bark ◽  
Hepatoprotective Activity ◽  
Normal Diet ◽  
High Dose ◽  
Animal House ◽  
Berberis Lycium

Background: To study the hepatoprotective effect of Ethanolic extract of Stem Bark of Berberis lycium Royale in isoniazid (INH) induced hepatotoxicity in mice model.Methods: The study design was lab based randomized controlled in-vivo study in mice conducted from 9th April 2014 till 9th May 2014 at animal house of National Institute of Health, Islamabad. Group A was on normal diet and water and hepatotoxicity was produced by giving isoniazid (50mg/kg BW) in mice of Group B. Group C and D were given isoniazid (INH) plus low dose and high dose of Ethanolic extract of stem bark of Berberis Lycium Royle respectively.Results: INH induced hepatotoxicity was depicted by elevated serum LFT’s, hepatocytic ballooning, severe steatosis and inflammation. Mice getting concurrent treatment of INH, low and high dose of Ethanolic extract of Berberis Lycium Royle showed decreased serum levels of biomarkers and their liver sections manifested improved histological picture but more significant reduction in toxic effects were observed in animals receiving high dose.Conclusions: High dose of Ethanolic extract of stem bark of Berberis lycium Royale showed more marked hepatoprotective activity as compare to low doses. The hepatotoxicity of INH can be reduced by concurrent use of INH with ethanolic extracts of Berberis Lycium Royle.

scholarly journals Evaluation of in vivo Hepatoprotective activity of Mimosa rubicaulis (LAM.) against CCL4 Induced Liver Toxicity

2021 ◽  
Vol 69 (1) ◽  
Author(s):  
Ashpak M. Tamboli ◽  
Kiran A. Wadkar
Keyword(s):  
Antioxidant Enzymes ◽  
Wistar Rats ◽  
Liver Toxicity ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Treated Animal ◽  
Ccl4 Administration ◽  
Female Wistar Rats

The current study was conducted to assess the hepatoprotective activity of different extracts of M. rubicaulis (Lam.) at (200 and 400 mg/kg) doses b.w. against CCl4 induced liver intoxication in Albino Wistar rats. Male or female Wistar rats about 150 and 200 gm body weight were selected for present study. The animals were divided into thirteen groups, six rats in each group. The extracts and Silymarin-treated animal groups significantly reduced the activities of various biochemical markers such as SGPT, SGOT, ALP, and TB which were elevated by carbon tetrachloride (CCl4) intoxication. The extracts of M. rubicaulis (Lam.) showed a dose dependent hepatoprotection activity. Among all the extracts, ethanolic extract produced maximum hepatoprotection (SGPT-83.15 %, SGOT76.82%, ALP-79.33%, TB-80.00%) at a 400mg/kg dose. After CCl4 administration, the levels of hepatic-antioxidant enzymes such as Glutathione (GSH) and Catalase (CAT) were reduced, whereas the level of hepatic lipid peroxidation (-LPO) increased. These hepatic antioxidant enzymes were also restored to normal levels by extracts and Silymarin treatment. The results of the present investigation indicate that all the extracts of M. rubicaulis (Lam.) possess hepatoprotective activity which may be due to the presence of various chemical constituents.

scholarly journals Upregulation of PDGF Mediates Robust Liver Regeneration after Nanosecond Pulsed Electric Field Ablation by Promoting the HGF/c-Met Pathway

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Junjie Qian ◽  
Jianpeng Liu ◽  
Liangjie Hong ◽  
Haohao Lu ◽  
Danjing Guo ◽  
...  
Keyword(s):  
Liver Function ◽  
Electric Field ◽  
Liver Regeneration ◽  
Molecular Mechanisms ◽  
Pulsed Electric Field ◽  
Serum Levels ◽  
Nanosecond Pulsed Electric Field ◽  
The Impact ◽  
Proliferation Of Hepatocytes

Nanosecond pulsed electric field (nsPEF) has emerged as a promising tool for hepatocellular carcinoma ablation recently. However, little is known about how nsPEF affects liver regeneration while being applied to eliminate liver lesions. Besides, the impact of nsPEF ablation on liver function should also be taken into consideration in the process. In this paper, we study the impact of nsPEF ablation on liver function by the measurement of serum levels of AST and ALT as well as liver regeneration and relevant molecular mechanisms in vivo. We found that mouse liver function exhibited a temporary injury without weight loss after ablation. In addition, local hepatic nsPEF ablation promoted significant proliferation of hepatocytes of the whole liver with an increase in HGF level. Moreover, the proliferation of hepatocytes was dramatically inhibited by the inhibitor of c-Met. Of interest, the periablational area is characterized by high level of PDGF and a large amount of activated hepatic stellate cells. Furthermore, neutralizing PDGF was able to significantly inhibit liver regeneration, the increased HGF level, and the accumulation of activated HSCs. Our findings demonstrated that nsPEF not only was a safe ablation approach but also could stimulate the regeneration of the whole liver through the activation of the HGF/c-Met pathway by upregulation of PDGF within the periablational zone.

scholarly journals A Herbal Mixture from Propolis, Pomegranate, and Grape Pomace Endowed with Anti-Inflammatory Activity in an In Vivo Rheumatoid Arthritis Model

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2255 ◽  
Author(s):  
Valentina Parisi ◽  
Antonio Vassallo ◽  
Claudio Pisano ◽  
Giacomo Signorino ◽  
Francesco Cardile ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Clinical Symptoms ◽  
Serum Levels ◽  
Grape Pomace ◽  
Inflammatory Factors ◽  
Campania Region ◽  
Anti Inflammatory ◽  
Inflammatory Autoimmune Disease ◽  
The Impact

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease characterized by the production of inflammatory factors. In order to overcome the side effects of currently used anti-inflammatory drugs, several attempts have been made to identify natural products capable of relieving RA symptoms. In this work, a herbal preparation consisting of propolis, pomegranate peel, and Aglianico grape pomace (PPP) extracts (4:1:1) was designed and evaluated for its effect on a murine collagen-induced arthritis (CIA) model. Firstly, the chemical contents of four different Italian propolis collected in the Campania region (Italy) were here reported for the first time. LC-MS analyses showed the presence of 38 constituents, identified in all propolis extracts, belonging to flavonoids and phenolic acids classes. The Pietradefusi extract was the richest one and thus was selected to design the PPP preparation for the in vivo assay. Our results highlight the impact of PPP on RA onset and progression. By using in vivo CIA models, the treatment with PPP resulted in a delayed onset of the disease and alleviated the severity of the clinical symptoms. Furthermore, we demonstrated that early PPP treatment was associated with a reduction in serum levels of IL-17, IL-1b, and IL-17–triggering cytokines.

scholarly journals Water Extract of Acori Graminei Rhizoma Attenuates Features of Rheumatoid Arthritis in DBA/1 Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Jong-Hyun Nho ◽  
A-Hyeon Kim ◽  
Ho-Kyung Jung ◽  
Mu-Jin Lee ◽  
Ji-Hun Jang ◽  
...  
Keyword(s):  
Liver Toxicity ◽  
Hematological Parameters ◽  
Water Extract ◽  
Inflammatory Cells ◽  
Type Ii Collagen ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type Ii

The dry rhizome of Acorus gramineus Solander, known as Acori Graminei Rhizoma, is used to treat dementia, stroke, eczema, and indigestion in traditional Chinese medicine, traditional Korean medicine, and traditional Japanese Kampo medicine. Previous studies have reported that Acori Graminei Rhizoma extract ameliorated cognitive impairment in Aβ1-42 injected mice. However, the effect of Acori Graminei Rhizoma on type II collagen induced arthritis (CIA) has not been elucidated. Thus, we evaluated the water extract of Acori Graminei Rhizoma (WAG) in CIA mice models. Male DBA/1 mice were separated into five groups (NOR; n=10, CON; n=10, CIA + methotrexate (MTX); n=10, CIA + 100 mg/kg WAG; n=10, CIA + 500 mg/kg WAG; n=10). CIA was induced by injecting the mice with bovine type II collagen, after which the mice were treated with WAG and/or MTX. Hematological parameters and liver and kidney serum toxicity markers were analyzed. Further, serum levels of interleukin (IL)-6, TNF-α, and type II collagen IgG were analyzed via enzyme-linked immunosorbent assay (ELISA). Treatment with 500 mg/kg WAG decreased serum levels of IL-6, TNF-α, and collagen IgG in a CIA model. Moreover, WAG treatment decreased CIA-induced swelling of mouse hind legs, infiltration of inflammatory cells into the synovial membrane, and blood neutrophil levels. WAG administration did not influence hematological parameters or kidneys and liver toxicity markers. WAG may be used to treat arthritis by reducing the inflammation indicators. However, further experiments are required to determine how WAG affects inflammation mechanisms in vitro and in vivo.

scholarly journals Folate-Equipped Cationic Liposomes Deliver Anti-MDR1-siRNA to the Tumor and Increase the Efficiency of Chemotherapy

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1252
Author(s):  
Daniil V. Gladkikh ◽  
Aleksandra V. Sen′kova ◽  
Ivan V. Chernikov ◽  
Tatyana O. Kabilova ◽  
Nelly A. Popova ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Chronic Toxicity ◽  
Liver Toxicity ◽  
Combined Therapy ◽  
Scid Mice ◽  
Tumor Xenograft ◽  
Folate Receptors ◽  
Immunodeficient Mice ◽  
Acute And Chronic Toxicity

In this study, we examined the in vivo toxicity of the liposomes F consisting of 1,26-bis(cholest-5-en-3-yloxycarbonylamino)-7,11,16,20-tetraazahexacosan tetrahydrochloride, lipid-helper 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine and folate lipoconjugate (O-{2-[rac-2,3-di(tetradecyloxy)prop-1-yloxycarbonyl]aminoethyl}-O’-[2-(pteroyl-L-glutam-5-yl)aminoethyl]octadecaethyleneglycol) and investigated the antitumor effect of combined antitumor therapy consisting of MDR1-targeted siMDR/F complexes and conventional polychemotherapy using tumor xenograft initiated in immunodeficient mice. Detailed analysis of acute and chronic toxicity of this liposomal formulation in healthy C57BL/6J mice demonstrated that formulation F and parent formulation L (without folate lipoconjugate) have no acute and chronic toxicity in mice. The study of the biodistribution of siMDR/F lipoplexes in SCID mice with xenograft tumors formed by tumor cells differing in the expression level of folate receptors showed that the accumulation in various types of tumors strongly depends on the abandons of folate receptors in tumor cells and effective accumulation occurs only in tumors formed by cells with the highest FR levels. Investigating the effects of combined therapy including anti-MDR1 siRNA/F complexes and polychemotherapy on a multidrug-resistant KB-8-5 tumor xenograft in SCID mice demonstrated that siMDR/F increases the efficiency of polychemotherapy: the treatment leads to pronounced inhibition of tumor growth, reduced necrosis and inflammation, and stimulates apoptosis in KB-8-5 tumor tissue. At the same time, it does not induce liver toxicity in tumor-bearing mice. These data confirm that folate-containing liposome F mediated the extremely efficient delivery of siRNA in FR-expressing tumors in vivo and ensured the safety and effectiveness of its action.

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