scholarly journals 1H-NMR Metabolic Profiling and Antioxidant Activity of Saffron (Crocus sativus) Cultivated in Lebanon

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4906
Author(s):  
Hala Samaha ◽  
Nathalie Chahine ◽  
Anatoly Petrovich Sobolev ◽  
Luigi Menghini ◽  
Hassane Makhlouf
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Crocus Sativus ◽  
Quantitative Composition ◽  
Good Discrimination ◽  
Adenocarcinoma Cells ◽  
Human Lung Adenocarcinoma Cells ◽  
High Antioxidant Activity ◽  
Saffron Extract ◽  
First Time

Despite the beneficial health properties shown by Lebanese saffron, its qualitative and quantitative composition has never been investigated before. In the present study, NMR spectroscopy, together with antioxidant activity assays, were applied to evaluate the chemical composition of saffron samples of different geographical origins (Lebanon, Italy, Iran, and India) and to categorize the Lebanese saffron for the first time. The distinction between Lebanese saffron and that produced in other countries was attributed to its higher linolenic and linoleic fatty acids, glucose and picrocrocin contents. Moreover, spices produced in three different regions of the Lebanese territory have been clearly differentiated. Saffron cultivated in the Qaa region displayed a high glucose, fatty acids and polyphenols content, whereas Hermel saffron exhibited the largest rate of picrocrocin and glycosylated carotenoids. Finally, samples from Baalbeck showed lower rates for the majority of metabolites. Moreover, Lebanese saffron showed a high antioxidant activity in ABTS and DPPH assays. A low dose of saffron extract (10 µg/mL) inhibited the growth of human lung adenocarcinoma cells, probably due to the high polyphenolic content. This study highlights the quality and peculiarity of Lebanese saffron cultivated in Northern Beqaa district and allows for a good discrimination between spices produced in relatively close territory.

scholarly journals Wild Mushrooms in Nepal: Some Potential Candidates as Antioxidant and ACE-Inhibition Sources

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Tran Hai Bang ◽  
Hiroto Suhara ◽  
Katsumi Doi ◽  
Hiroya Ishikawa ◽  
Katsuya Fukami ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Ace Inhibition ◽  
Promising Candidate ◽  
Phenolic Contents ◽  
High Antioxidant Activity ◽  
First Time ◽  
Ganoderma Lingzhi ◽  
Very High

Twenty-nine mushrooms collected in the mountainous areas of Nepal were analyzed for antioxidant activity by different methods, including Folin-Ciocalteu, ORAC, ABTS, and DPPH assays. Intracellular H2O2-scavenging activity was also performed on HaCaT cells. The results showed that phenolic compounds are the main antioxidant of the mushrooms. Among studied samples,Inonotus andersonii, andPhellinus gilvusexhibited very high antioxidant activity with the phenolic contents up to 310.8 and 258.7 mg GAE/g extracts, respectively. The H2O2-scavenging assay on cells also revealed the potential of these mushrooms in the prevention of oxidative stress. In term of ACE-inhibition, results showed thatPhlebia tremellosawould be a novel and promising candidate for antihypertensive studies. This mushroom exhibited even higherin vitroACE-inhibition activity thanGanoderma lingzhi, with the IC50values of the two mushrooms being 32 μg/mL and 2 μg/mL, respectively. This is the first time biological activities of mushrooms collected in Nepal were reported. Information from this study should be a valuable reference for future studies on antioxidant and ACE-inhibitory activities of mushrooms.

scholarly journals Antioxidant and Antiglycation Properties of Seventeen Fruit Teas Obtained from One Manufacturer

2020 ◽  
Vol 10 (15) ◽  
pp. 5195 ◽  
Author(s):  
Izabela Zieniewska ◽  
Anna Zalewska ◽  
Małgorzata Żendzian-Piotrowska ◽  
Jerzy Robert Ładny ◽  
Mateusz Maciejczyk
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Aromatic Amino Acids ◽  
Raw Material ◽  
Quantitative Composition ◽  
Production Location ◽  
Dried Fruit ◽  
Health Promoting ◽  
High Antioxidant Activity

The antioxidant activity of teas depends on the type and quality of the ingredients used in the process of tea production, location of the crops, and manner of the raw material processing. Our study is the first to compare the antioxidant and antiglycation properties of seventeen fruit teas obtained from one manufacturer. We evaluated three different brewing times (3, 5, and 10 min) and two brewing temperatures (70 and 100 °C). We demonstrated that infusions with the longest brewing time reveal the highest antiradical activity, while increased brewing temperature does not significantly affect the assessed parameters. The best antioxidant properties were obtained for the teas made from lemon balm with pear, forest fruits, cranberry with pomegranate, raspberry, and raspberry with linden. Fruit teas owe their high antioxidant activity to the presence of polyphenolic compounds in infusions. Extracts from fruit teas also diminish the oxidation and glycation of albumin in vitro, observed as a decrease in the fluorescence of aromatic amino acids and advanced glycation (AGE) and oxidation (AOPP) protein products levels. In conclusion, in order to prepare fruit teas with the best antioxidant properties, a longer extraction time is needed. The health-promoting properties of dried fruit infusions can be modified by changing the qualitative and quantitative composition of the ingredients.

scholarly journals Chia (Salvia hispanica): Nutraceutical Properties and Therapeutic Applications

Proceedings ◽  
2020 ◽  
Vol 53 (1) ◽  
pp. 17
Author(s):  
Talía Hernández-Pérez ◽  
María Elena Valverde ◽  
Domancar Orona-Tamayo ◽  
Octavio Paredes-Lopez
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Total Phenolics ◽  
Human Diet ◽  
Salvia Hispanica ◽  
Nutraceutical Compounds ◽  
Fatty Acids Profile ◽  
High Antioxidant Activity ◽  
Salvia Hispanica L ◽  
Nutraceutical Properties

Chia seeds (Salvia hispanica L.) have high amounts of nutraceutical compounds and a great commercial potential. The aim of this work was to identify proximate composition, fatty acids profile, total phenolics and antioxidant capacity of chia, as well the protein fractions and determine their antihypertensive potential. The seeds exhibited high content of protein, fiber, and lipids, mainly polyunsaturated fatty acids. Important amounts of phenols and a high antioxidant activity (DPPH and ABTS) were found. Globulins fraction showed the most abundant concentration followed by albumins. Peptides from albumins and globulins exhibited the strongest potential against the angiotensin-converting enzyme (ACE) activity. In brief, this study demonstrates that chia can be considered a seed with high nutritional content, antioxidant activity and as a novel antihypertensive agent; important factors for the frequent incorporation of chia in the human diet.

scholarly journals Physical-chemical, nutritional and antioxidant properties of tucumã (Astrocaryum huaimi Mart.) fruits

2018 ◽  
Vol 39 (4) ◽  
pp. 1517
Author(s):  
Mirelly Marques Romeiro Santos ◽  
Dayane Stéphanie Fernandes ◽  
Camila Jordão Cândido ◽  
Leandro Fontoura Cavalheiro ◽  
Anderson Fernandes da Silva ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Unsaturated Fatty Acids ◽  
Antioxidant Properties ◽  
Nutritional Deficiencies ◽  
Total Dietary Fiber ◽  
Physical Chemical ◽  
High Antioxidant Activity

The Brazilian Cerrado contains a large number of fruit species whose nutritional and physical-chemical properties have not been fully characterized to date. The fruit of tucumã (Astrocaryum huaimi Mart) is ellipsoid with fibrous and gelatinous pulp, unique odor, and has significant economic importance in the region. The objective of this study is to analyze the physical-chemical, nutritional, and antioxidant properties of the fruit of tucumã grown in Minas Gerais state, Brazil. Water content, ashes, proteins, lipids, carbohydrates, total dietary fiber, pH, titratable acidity, soluble solids, vitamin C, minerals, and fatty acids were analyzed. Total phenols, tannins, and antioxidant activity were analyzed using the Folin-Ciocalteau method, Folin-Denis method, and photocolorimetric method of the stable free radical DPPH, respectively, in aqueous, acetone, and ethanol extracts. The most relevant components in the fruit were lipids, fibers, vitamin C, unsaturated fatty acids, iron, potassium, manganese, and bioactive compounds. The analysis of the bioactive compounds revealed that the fruit has high antioxidant activity. The acetone extract presented the highest antioxidant capacity followed by the ethanol and aqueous extracts. The results indicated that the tucumã fruit has high nutritional value as a source of lipids, fibers, calories, vitamin C, minerals, and unsaturated fatty acids, and high antioxidant activity. Therefore, consuming the tucumã fruit pulp may help prevent nutritional deficiencies and chronic non-communicable diseases.

scholarly journals LIPIDS, FATTY ACIDS AND PIGMENTS OF LEAVES DICTAMNUS CAUCASICUS FISCH. EX GROSSH. (RU-TACEAE)

2020 ◽  
pp. 233-238
Author(s):  
Elena Sergeyevna Bogdanova ◽  
Viktor Nikolayevich Nesterov ◽  
Stepan Aleksandrovich Senator ◽  
Vladimir Mikhaylovich Vasjukov ◽  
Ol'ga Anatol'yevna Rozentsvet
Keyword(s):  
Fatty Acids ◽  
High Performance ◽  
Neutral Lipids ◽  
Chemical Compounds ◽  
Quantitative Composition ◽  
Gas Liquid Chromatography ◽  
Molybdenum Blue ◽  
Qualitative And Quantitative ◽  
Anthrone Reagent ◽  
First Time

Plants of the genus Dictamnus are a source of chemical compounds with different biological activity – alkaloids, bitterness, furocoumarins, saponins, essential oils. In the present work, the qualitative and quantitative composition of components lipids, fatty acids, and pigments isolated from the leaves of Dictamnus caucasicus Fisch. ex Grossh. – is presented for the first time, also of interest for medicine and pharmacology. Plants were selected in the Samara region, on the territory of which D. caucasicus is currently under threat of extinction. Plant extracts were analyzed by high performance thin-layer and gas-liquid chromatography. Identification of polar lipids was carried out using specific reagents – anthrone reagent, molybdenum blue, Dragendorff’s reagent, 0.2% ninhydrin solution. Neutral lipids were identified using an mixture of standards – triacylglycerol (TAG), oleic acid (FFA), cholesterol (ST), diacyglycerol (DAG). It was shown that the main group of lipids contains carbohydrate fragments – glycolipids as structural components. Among phospholipids, a high content of phosphatidylglycerol was found, and in neutral lipids – free and esterified forms of sterols. Polyunsaturated linolenic acid (C18: 3n3) dominates in the composition of fatty acids, and chlorophyll a dominates in the pigment pool.

scholarly journals Determination of fatty acid composition and antioxidant activity in vegetable oils

Chemija ◽  
2021 ◽  
Vol 32 (1) ◽  
Author(s):  
Daiva Kazlauskienė ◽  
Giedrė Kasparavičienė ◽  
Palma Nenortienė ◽  
Mindaugas Marksa ◽  
Julija Jukilaitytė ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Vegetable Oils ◽  
Sesame Seed ◽  
Omega 3 ◽  
Hemp Seed ◽  
Spectrophotometric Methods ◽  
Study Results ◽  
Omega 6 ◽  
High Antioxidant Activity

Studies of the composition of vegetable oils are expedient in order to achieve quality product modelling. The goal of this research was to determine the composition of fatty acids using gas chromatography (GC) and antioxidant activity using DPPH and ABTS spectrophotometric methods. Analysed oils were castor seed, sunflower seed, sesame seed, fibrous hemp seed, black radish seed and olive oils. Different classes of fatty acids were determined: polyunsaturated, monounsaturated and saturated; omega-3, omega-6 and omega-9 acids and individual constituents. The study results showed that the dominant acids in all oils were oleic and linoleic fatty acids. The best ratio of omega-6 to omega-3 acids was found in fibrous hemp seed oil – 3:1. All analysed vegetable oils demonstrated a high antioxidant activity.

UJI IMUNOSTIMULATOR DARI KOMBINASI EKSTRAK ETANOL HERBA BINARA (ARTEMISIA VULGARIS LINN) DAN EKSTRAK ETANOL DAUN PUCUK MERAH (SYZYGIUM OLEANA) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT PADA TIKUS JANTAN

2019 ◽  
Vol 1 (2) ◽  
pp. 22-26
Author(s):  
Romauli Anna Teresia Marbun ◽  
Aminah Syarifuddin ◽  
Montysory Silalahi ◽  
Radika Bella Fista Ginting
Keyword(s):  
Antioxidant Activity ◽  
Immune System ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Positive Control ◽  
Artemisia Vulgaris ◽  
Chemical Screening ◽  
Chemical Content ◽  
High Antioxidant Activity ◽  
New Treatments

Diseases mediated by the immune system are difficult problems to treat such as human immunodeficiency virus (HIV) and other lethal viruses. Infections that occur in normal people are generally brief and rarely leave permanent damage. Treatment of this disease requires an aggressive and innovative approach to the development of new treatments so that it requires the role of immunomodulators to improve the immune system. A substance that acts as an enhancer or immune enhancer can be obtained by using herbs that are efficacious as immunostimulants. One of the herbs used is herbal binara (Artemisia vulgaris L) which has been studied as a potential immunomodulator with high antioxidant activity. Previous research also stated that red shoots (Syzygium oleana) were studied as potential immunomodulators with high antioxidant activity. Several other species such as Syzygium samarangense have 16 flavonoida compounds which show pharmacological immunological activity. The purpose of this study was to determine the content of secondary metabolites of ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) and to determine the best dose of extract from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) can reduce the volume of swelling of mouse feet. Examination of the chemical content of secondary metabolites from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) is carried out by chemical screening and characterization of simplicia and extract. The method used is the slow type hypersensitivity method. In this test the independent variable is the secondary metabolite of ethanol extract of herb binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) with four concentrations (50, 100, 200 and 400 mg / kgBB). The positive control used by Stimuno dose is 32.5 mg / kgBB

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