Silver Nanoparticles from Annona muricata Peel and Leaf Extracts as a Potential Potent, Biocompatible and Low Cost Antitumor Tool

Cancer is one of the most prevalent diseases in the world and requires new therapies for its treatment. In this context, the biosynthesis of silver nanoparticles (AgNPs) has been developed to treat different types of tumors. The Annona muricata plant is known for having anticancer activity. Its main compounds present in the leaves, stems and skin, allowing for its use as reducing agents. In this manuscript, AgNPs with leaf extract (AgNPs-LE) and fruit peel extract (AgNPs-PE) of A. muricata were biosynthesized obtaining an average nanoparticle diameter sizes smaller than 50 nm, being 19.63 ± 3.7 nm and 16.56 ± 4.1 nm, and with a surface plasmonic resonance (SPR) at 447 and 448 nm, respectively. The lactone functional group present in the LE and PE extracts was identified by the FTIR technique. The behavior and antiproliferation activity of AgNPs-LE and AgNPs-PE were evaluated in breast, colon and melanoma cancer cell lines. Our results showed that Annona muricata fruit peel, which is a waste product, has an antitumor effect more potent than leaf extract. This difference is maintained with AgNPs where the destruction of cancer cells was, for the first time, achieved using concentrations that do not exceed 3 μg/mL with a better therapeutic index in the different tumor strains. In conclusion, we present a low-cost one-step experimental setup to generate AgNPs-PE whose in-vitro biocompatibility and powerful therapeutic effect make it a very attractive tool worth exploiting.

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In–Vitro Antibacterial and Antifungal Efficacy of Greenly Fabricated Senna alata Leaf Extract Silver Nanoparticles and Silver Nanoparticle-Cream Blend

Periodica Polytechnica Chemical Engineering ◽

10.3311/ppch.18271 ◽

2022 ◽

Author(s):

Bukola Christianah Adebayo-Tayo ◽

Samuel Oluwadara Borode ◽

Solomon Omoniyi Alao

Keyword(s):

Silver Nanoparticles ◽

Silver Nanoparticle ◽

Pathogenic Bacteria ◽

Leaf Extract ◽

Aryl Ether ◽

Leaf Extracts ◽

Bacteria And Fungi ◽

Antibacterial And Antifungal ◽

Senna Alata

Nanoparticles biosynthesis has been extensively studied for its biomedical applications. In this study, the in-vitro antibacterial and antifungal activity of greenly fabricated silver nanoparticles (NPs) from Senna alata leaf extract (SaAgNPs) and silver nanoparticle cream blend (SaAgNPs-cream blend) were investigated. The SaAgNPs were characterized using UV-visible spectrophotometry, FTIR, SEM, TGA, DLS, EDX, and XRD. The presence of surface plasmon band around 500 nm indicates AgNPs formation and functional groups such as alkenes, carboxylic acids, and alkyl aryl ether responsible for capping and stabilization of the nanoparticles. The SaAgNPs were spherical and 1.00 µm in size; TGA shows the formation of stable SaAgNPs, DLS shows 1.8 % intensity with 1905 nm average diameter and a polydispersity index of 0.595. EDX analysis confirmed the formation of pure silver nanoparticles. SaAgNPs supported the production of cosmetically acceptable SaAgNPs-cream blend with appropriate pH and viscosity. SaAgNPs and the SaAgNPs cream-blends had antibacterial activity against all and some of the test bacterial and fungal isolates. SaAgNPs had the highest activity against Pseudomonas aeruginosa 27853, Rhizopus sp. and Candida tropicalis with a zone of 16 mm and 30 mm. The cream-blends had activity against 68.75 % and 75 % of the test bacteria and fungi with the highest activity against Streptococcus epidermidis B (7.0 mm) and Candida albicans B (8.0 mm). In conclusion, the crude Senna alata leaf extracts, the bio-fabrication SaAgNPs and SaAgNPs-cream blend have antibacterial antifungal potentials which can be effectively utilized for the control of pathogenic bacteria and fungi.

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Size-controlled fabrication of silver nanoparticles using the Hedyotis puberula leaf extract: toxicity on mosquito vectors and impact on biological control agents

RSC Advances ◽

10.1039/c6ra23208f ◽

2016 ◽

Vol 6 (99) ◽

pp. 96573-96583 ◽

Cited By ~ 7

Author(s):

Raja Mohamed Sait Thameem Azarudeen ◽

Marimuthu Govindarajan ◽

Abubucker Amsath ◽

Shine Kadaikunnan ◽

Naiyf S. Alharbi ◽

...

Keyword(s):

Biological Control ◽

Silver Nanoparticles ◽

Leaf Extract ◽

Low Cost ◽

Aquatic Organisms ◽

Capping Agent ◽

Mosquito Vectors ◽

Biological Control Agents ◽

Size Controlled ◽

Control Tool

As a low-cost and eco-friendly control tool, Ag nanoparticles were fabricated usingHedyotis puberulaaqueous extract as a reducing and capping agent and showed potent activity against malaria and arbovirus vectors with low biotoxicity against non-target aquatic organisms.

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Optimization of Green Synthesis of Silver Nanoparticles from Leaf Extracts ofPimenta dioica(Allspice)

The Scientific World JOURNAL ◽

10.1155/2013/362890 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 5

Author(s):

Akshay Rajeev Geetha ◽

Elizabeth George ◽

Akshay Srinivasan ◽

Jameel Shaik

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Leaf Extract ◽

Synthesis Method ◽

Leaf Extracts ◽

Synthesis Of Nanoparticles ◽

Hot Air ◽

Vis Spectroscopy ◽

Pimenta Dioica ◽

First Time

Production of silver nanoparticles from the leaf extracts ofPimenta dioicais reported for the first time in this paper. Three different sets of leaves were utilized for the synthesis of nanoparticles—fresh, hot-air oven dried, and sun-dried. These nanoparticles were characterized using UV-Vis spectroscopy and AFM. The results were diverse in that different sizes were seen for different leaf conditions. Nanoparticles synthesized using sun-dried leaves (produced using a particular ratio (1 : 0.5) of the leaf extract sample and silver nitrate (1 mM), resp.) possessed the smallest sizes. We believe that further optimization of the current green-synthesis method would help in the production of monodispersed silver nanoparticles having great potential in treating several diseases.

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A Bottom-Up Synthesis Approach to Silver Nanoparticles Induces Anti-Proliferative and Apoptotic Activities Against MCF-7, MCF-7/TAMR-1 and MCF-10A Human Breast Cell Lines

Molecules ◽

10.3390/molecules25184332 ◽

2020 ◽

Vol 25 (18) ◽

pp. 4332

Author(s):

Nurul Izzati Zulkifli ◽

Musthahimah Muhamad ◽

Nur Nadhirah Mohamad Zain ◽

Wen-Nee Tan ◽

Noorfatimah Yahaya ◽

...

Keyword(s):

Breast Cancer ◽

Silver Nanoparticles ◽

Cell Lines ◽

Leaf Extract ◽

Breast Cancer Cell Lines ◽

Face Centered Cubic ◽

Human Breast ◽

Bottom Up ◽

Mcf 7

A bottom-up approach for synthesizing silver nanoparticles (AgNPs-GA) phytomediated by Garcinia atroviridis leaf extract is described. Under optimized conditions, the AgNPs-GA were synthesized at a concentration of 0.1 M silver salt and 10% (w/v) leaf extract, 1:4 mixing ratio of reactants, pH 3, temperature 32 °C and 72 h reaction time. The AgNPs-GA were characterized by various analytical techniques and their size was determined to be 5–30 nm. FTIR spectroscopy indicates the role of phenolic functional groups in the reduction of silver ions into AgNPs-GA and in supporting their subsequent stability. The UV-Visible spectrum showed an absorption peak at 450 nm which reflects the surface plasmon resonance (SPR) of AgNPs-GA and further supports the stability of these biosynthesized nanoparticles. SEM, TEM and XRD diffractogram analyses indicate that AgNPs-GA were spherical and face-centered-cubic in shape. This study also describes the efficacy of biosynthesized AgNPs-GA as anti-proliferative agent against human breast cancer cell lines, MCF-7 and MCF-7/TAMR-1. Our findings indicate that AgNPs-GA possess significant anti-proliferative effects against both the MCF-7 and MCF-7/TAMR-1 cell lines, with inhibitory concentration at 50% (IC50 values) of 2.0 and 34.0 µg/mL, respectively, after 72 h of treatment. An induction of apoptosis was evidenced by flow cytometry using Annexin V-FITC and propidium iodide staining. Therefore, AgNPs-GA exhibited its anti-proliferative activity via apoptosis on MCF-7 and MCF-7/TAMR-1 breast cancer cells in vitro. Taken together, the leaf extract from Garcinia atroviridis was found to be highly capable of producing AgNPs-GA with favourable physicochemical and biological properties.

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The PHYTOCHEMICALS, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Senna alata AND Senna tora LEAF EXTRACTS AGAINST BACTERIAL STRAINS CAUSING SKIN INFECTIONS

Bacterial Empire ◽

10.36547/be.2019.2.1.19-25 ◽

2019 ◽

Vol 2 (1) ◽

pp. 19

Author(s):

Murni Halim

Keyword(s):

Leaf Extract ◽

Antimicrobial Properties ◽

Inhibition Zone ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Bacterial Strains ◽

Leaf Extracts ◽

Natural Drugs ◽

Senna Alata

A study was carried out to screen for phytochemical constituents and assess the antioxidant and antimicrobial activities of Senna alata and Senna tora leaf extracts. The leaves were first dried at room temperature and 50°C in an oven prior to solvent extraction using ethanol and methanol. The in-vitro qualitative assays showed that both S. alata and S. tora leaf extracts contained bioactive and secondary metabolites components such as tannins, steroids, saponin, terpenoids, glycosides, flavonoids and phenols. The antioxidant activity and capacity test were carried out by conducting free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Ferric reduction antioxidant plasma (FRAP) assays. Both assays showed S. tora leaf extract has higher antioxidant capacity than S. alata leaf extract. The efficacy of these leaf extracts were tested against skin pathogens through agar well diffusion method. S. alata extract showed an inhibition zone (1.15 – 1.59 mm) against Pseudomonas aeruginosa while S. tora extracts exhibited a strong antimicrobial activity against S. epidermidis (inhibition zone of 12 – 16.94 mm) followed by P. aeruginosa (inhibition zone of 1 – 1.59 mm). Nonetheless, no inhibition zone was observed for S. aureus by both leaf extracts. The phytochemicals and antioxidant constituents as well as inhibitory potential on skin pathogens possessed by S. alata and S. tora leave highlighted their potential utilization in the development of natural drugs or cosmetics to treat skin related diseases or infections.

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In Vitro Antimicrobial Activity of Zodia (Evodia suaveolens) Leaf Extract on Pathogenic Agents Dragon Fruit Plant

Jurnal Biota ◽

10.19109/10.19109/biota.v6i2.6236 ◽

2020 ◽

Vol 6 (2) ◽

pp. 78-85

Author(s):

Trio Ageng Prayitno ◽

Nuril Hidayati

Keyword(s):

Antimicrobial Activity ◽

Leaf Extract ◽

Plant Pathogens ◽

Inhibition Zone ◽

Diffusion Method ◽

Leaf Extracts ◽

Dragon Fruit ◽

Fruit Plants ◽

Pathogenic Agents

The use of antimicrobials from plant extracts has not been used optimally to control pathogenic agents in dragon fruit plants. The purpose of this research was to determine the antimicrobial activity of zodia (Evodia suaveolens) leaf extracts on pathogenic agents of dragon fruit plants in vitro. The research method is laboratory research with Completely Randomized Design (CRD). The antimicrobial concentrations of zodia (E. suaveolens) leaf extract used six types including 50%, 60%, 70%, 80%, 90%, and 100% with four replications. The research sample was the leaf of zodia (E. suaveolens), Pseudomonas aeruginosa and Fusarium oxysporum strain Malang. Test the antimicrobial activity of zodia (E. suaveolens) leaf extracts on the growth of P. aeruginosa and F. oxysporum using the disc-diffusion method and wells method. The research instrument was used the observation sheet of the diameter of inhibition zone indicated by the clear zone. The diameter of inhibition zone data were analyzed using the One Way ANOVA test. The results showed that the antimicrobial activity of zodia (E. suaveolens) leaf extract significantly inhibited the growth of P. aeruginosa and F. oxysporum (P <0.05). These results recommend zodia (E. suaveolens) leaf extract as an antimicrobial agent for dragon fruit plant pathogens.

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Phytochemical evaluation and in vitro antidiabetic efficiency of isopropanolic leaf extract of pimenta racemosa

Kongunadu Research Journal ◽

10.26524/krj.2020.21 ◽

2020 ◽

Vol 7 (2) ◽

pp. 50-55

Author(s):

Anitha T A ◽

Pakutharivu T ◽

Nirubama K ◽

Akshaya V

Keyword(s):

Diabetes Mellitus ◽

Glucose Uptake ◽

Inhibitory Activity ◽

Leaf Extract ◽

Yeast Cells ◽

Antidiabetic Activity ◽

Leaf Extracts ◽

Pimenta Racemosa

The traditional herbal medicines are mainly obtained from plants are used in the management of Diabetes mellitus. The main objective of this work was to assess the presence of phytochemical compounds and to evaluate the in vitro antidiabetic activity of isopropanolic extracts of Pimenta racemosa leaves by studying their α-amylase inhibitory activity and glucose transport across yeast cells. Screening of phytochemicals showed positive results for alkaloids, steroids, cardiac glycosides, terpenoids, reducing sugars, anthraquinones, and results of in vitro α-amylase inhibitory studies demonstrated there was a dose-dependent increase in percentage inhibitory activity by the isopropanolic leaf extracts of Pimenta racemosa. At a concentration of 1 mg/ml, the extract showed a percentage inhibition 33.6 and for 5 mg/ml it was 91.2. The glucose uptake study was also studied through yeast cells by analyzing theamount of glucose remaining in the medium after a specific time intervals. It serves as an indicator for the capability of isopropanolic leaf extracts of Pimenta racemosa to transport the glucose into yeast cells. As a result, we found that the isopropanolic leaf extract of Pimenta racemosa have inhibitory activity against αamylase and also, which is efficient in glucose uptake. This therapeutic potentiality of Pimenta racemosa could be exploited in the treatment of Type 2 Diabetes mellitus. Further studies are also required to elucidate whether the plant have antidiabetic potential by in vivo for corroborating the traditional claim of the plant.

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