scholarly journals Effect of Germinated Pigmented Rice “Superjami” on the Glucose Level, Antioxidant Defense System, and Bone Metabolism in Menopausal Rat Model

Nutrients ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 2184
Author(s):  
Soo Im Chung ◽  
Tae-ho Ham ◽  
Mi Young Kang
Keyword(s):  
Body Weight ◽  
Bone Metabolism ◽  
Glucose Level ◽  
Rat Model ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Rice Cultivar ◽  
The Body ◽  
Defense System ◽  
Pigmented Rice

Women experience physical, mental, and social changes during menopause. It is important to maintain a healthy diet for effective menopause management. The effect of germinated Superjami, a deep violet colored rice cultivar, on the body weight, glucose level, antioxidant defense system, and bone metabolism in a menopausal rat model was investigated. The animals were randomly divided into three groups and fed with a normal diet (ND), a control diet supplemented with 20% (w/w) non-germinated Superjami flour (NGSF), or germinated Superjami flour (GSF) for eight weeks. The NGSF and GSF groups exhibited significantly lower body weight and fat, glucose and insulin contents, adipokine concentrations, and bone resorption biomarker levels, and higher antioxidant enzyme activities and 17-β-estradiol content than the ND group (p < 0.05). The GSF group showed greater glucose homeostasis, antioxidative, and bone metabolism-improving effects compared with the NGSF group. These findings demonstrate that germination could further improve the health-promoting properties of Superjami and that this germinated pigmented rice cultivar could be useful in the treatment and management of menopause-induced hyperglycemia, oxidative stress, and bone turnover imbalance.

Manganese and Zinc Overdose - Risk of Oxidative Stress Appearance

2008 ◽  
Vol 59 (9) ◽  
Author(s):  
Mirela Ahmadi ◽  
Mihaela Pup ◽  
Lucia Olariu ◽  
Horia Vermesan ◽  
Radu Prejbeanu
Keyword(s):  
Oxidative Stress ◽  
Trace Metals ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
The Body ◽  
Hepatic Iron ◽  
Defense System ◽  
Amplification Effect ◽  
Enzymes Activity ◽  
Manganese Level

The study presents the effect of Zn and Mn overdoses in relation with other trace metals in rat�s liver. All studied metals are involved in some enzymes activity, which are implicated in antioxidant defense system of the body. But, in some conditions � our case in overdoses � an imbalance can appear, and antioxidant character of a metal can become prooxidant. In this case, antagonistic or synergic behaviour in relation with other trace metals is obvious. The observed modifications are the increasing of zinc and manganese level in liver after solutions administration, and the amplification effect over the hepatic iron, with the possibility of oxidative stress appearance.

scholarly journals NONINVASIVE METHODS FOR STUDYING THE INFLUENCE OF NONFERROUS METALLURGY EMISSIONS ON HUMAN HEALTH

2019 ◽  
Vol 96 (12) ◽  
pp. 1192-1195 ◽  
Author(s):  
V. N. Rakitskii ◽  
T. V. Yudina ◽  
Marina V. Egorova ◽  
N. S. Kutakova
Keyword(s):  
Heavy Metals ◽  
Trace Elements ◽  
Health Risk ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Oxidative Status ◽  
Nonferrous Metallurgy ◽  
The Body ◽  
Defense System ◽  
Methodological Approaches

There are presented methodological approaches to assess the risk of negative factors associated with characteristic areas of metallurgical enterprises in pollution of the environment with heavy metals to human health. The use of noninvasive biotesting, based on established correlations of indices of trace elements and oxidative status in the body is suggested. As a non-invasive biological material, alveolar moisture condensate from the person (expirate) is used. The basis for the health risk assessment is information on the balance of two components of the oxidative process responsible for the adaptive function of the body - free radical oxidation and antioxidant defense system. An imbalance of these processes contributes to the development of oxidative stress, the complication of the course of many diseases. The deviation in quantitative indices of a number of elements, primarily of metals of variable valency, which participate in reactions of lipid peroxidation, showing pro - or antioxidant properties can also serve as the indicator of the effects of complex heavy metals. The identified relationship of trace elements and oxidative status allows providing the approach to the assessment of processes of peroxidation in the assessment of microelement exchange indirectly. To study the intensity of peroxidation and capacity of the antioxidant defense system in expirate a number of methodological solutions was proposed - methods of chemiluminescence and spectrophotometry, as well as biotest, allowing detect deviations in the oxidative status and based on the level of bioluminescence of lyophilized bacteria by oxidative potential of investigated expirate of alveolar moisture. The complex of methods for assessment of the degree of the health risk associated with pollution by heavy metals can be used for screening observations in selected contingents with the purpose of formation of risk groups such as individually for the choice the optimal ways of correction of disorders and the assessment of the efficiency of preventive or corrective measures. Examples of methodological approaches in one of the regions of accommodation of the enterprises of nonferrous metallurgy, and in the estimation of risk development of pathological conditions and the efficiency of the treatment for a particular worker of the metallurgical plant, as the patient in the clinic of occupational diseases.

scholarly journals Activity of free radical oxidation processes in rats with paracetamol hepatitis and correction with shiitake mushrooms thick extract

2021 ◽  
pp. 81-90
Author(s):  
І. І. Герасимець ◽  
Л. С. Фіра ◽  
І. І. Медвідь
Keyword(s):  
Superoxide Dismutase ◽  
Body Weight ◽  
Free Radical ◽  
Acute Hepatitis ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Oxidative Modification ◽  
Male Rats ◽  
Defense System ◽  
Shiitake Mushrooms

The antioxidant defense system controls and inhibits all stages of free radical formation reactions, which start from their initiation and end with the formation of hydroperoxides and malonic dialdehyde. Disorders of the body's antioxidant defense system can be caused by both endogenous and exogenous factors and can lead to the oxidative stress development. For the purpose of the negative impact minimizing and correction of the existing disorders, both natural and synthetic origin antioxidants are used. Preference is given to antioxidants of natural origin, due to their mild action and minimal side effects. Shiitake mushrooms are known in China and Japan as a product that has nutritional and medicinal value, for thousands of years. They contain an extremely rich complex of various biologically active substances that can have antioxidant, hepatoprotective effect and influence on the liver diseases pathogenesis. The aim of the work was to investigate the effect of shiitake mushrooms thick extract on the activity of free radical processes and indicators of the antioxidant system under the conditions of paracetamol hepatitis in rats. The experimental work was being conducted on the white male rats, which were divided into 10 groups of 6 animals each. Acute hepatitis was simulated by intragastric administration of paracetamol in a dose of 1 250 mg/kg 1 time per day (for 2 days) as a suspension in 2% starch gel solution. We investigated the effect of shiitake mushrooms thick extract on the induced pathology, which was administered intragastrically 2 hours before the paracetamol introduction and daily after the lesion in a dose of 150 mg/kg of the rat’s body weight. Silibor was selected as the comparison drug, which was administered according to the same scheme like the investigated extract in a dose of 20 mg/kg of the animal’s body weight. Euthanasia was conducted on the 3rd, 7th and 10th day after the onset of the lesion. Liver homogenate and animal serum were examined. The effectiveness of shiitake mushrooms thick extract was evaluated under the conditions of the caused pathology by superoxide dismutase and catalase activity, as well as by the content of TBA-active and proteins oxidative modification products. An increasing of the TBA-active products content and the products of proteins oxidative modification in the serum and liver of animals after the toxic paracetamol exposure testifies to the liver damage and the development of acute hepatitis in rats. Decreased activity of antioxidant enzymes such as catalase and superoxide dismutase was also noted. An investigation, which was conducted by us, allowed us to reveal a positive effect of shiitake mushrooms thick extract on the activity of lipoperoxidation and oxidative modification of proteins under the conditions of white rats paracetamol lesion.

Baccharis trimera Improves the Antioxidant Defense System and Inhibits iNOS and NADPH Oxidase Expression in a Rat Model of Inflammation

2014 ◽  
Vol 14 (11) ◽  
pp. 975-984 ◽  
Author(s):  
Bruno Cruz Padua ◽  
Joamyr Rossoni Junior ◽  
Cíntia de Brito Magalhaes ◽  
Janaina Seiberf ◽  
Carolina Araujo ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Rat Model ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Baccharis Trimera

scholarly journals System of antioxidant protection of the body of piglets under the action of feed additive “Butaselmevit-plus”

2021 ◽  
Vol 4 (2) ◽  
pp. 38-43
Author(s):  
T. V. Martyshuk ◽  
B. V. Gutyj ◽  
V. I. Khalak
Keyword(s):  
Body Weight ◽  
Antioxidant Defense ◽  
Vena Cava ◽  
Antioxidant Defense System ◽  
The Body ◽  
Feed Additive ◽  
Before And After ◽  
Experimental Group ◽  
Technological Stress ◽  
Protective Systems

The aim of the study was to investigate the effect of the feed additive “Butaselmevit-plus” on the state of the antioxidant defense system of piglets during weaning. The experiments were conducted on the basis of the LTD “KOSHET” Mukachevo district of Zakarpattia region. Two groups of piglets were formed – control and experimental, in the amount of 10 individuals in each group, selected on the principle of analogs – age, breed and body weight. Animals were fed according to the norms for this age of pigs. On the 28th day of life, the piglets were weaned from the sow and regrouped from different nests for further maintenance during fattening and rearing with a change in the structure of the diet, which served as technological stress for the animal's body. Piglets of the experimental group, from 21 to 40 days of age, were additionally fed the feed additive “Butaselmevit-plus” at a dose of 100 mg/kg body weight per day. The material for the study was blood, which was taken in the morning before feeding the animals by puncture of the cranial vena cava for 20 days of life (the period before weaning), for 25 days of life (the period before weaning), for 30 days of life (2 days after weaning), for 35 days of life (7 days after weaning), 40 days of life (12 days after weaning). It is proved that feeding piglets feed additive “Butaselmevit-plus” enhanced the antioxidant status of piglets after weaning. It was found that under conditions of technological stress, the use of feed additive “Butaselmevit-plus” in the amount of 100 mg/kg body weight per day improves the antioxidant defense of the body, as evidenced by an increase in blood 35-day-old piglets superoxide dismutase activity by 26.7 % (P < 0.001), catalase – by 39.5 % (P < 0.001), glutathione peroxidase – by 51.7 % (P < 0.05), glutathione reductase – by 52.9 % (P < 0.05), the content of reduced glutathione – at 58.3 % (P < 0.001). These results confirm the effectiveness of the use of milk thistle, methionine, selenium, and vitamins A, E, and D3 in the feed additive “Butaselmevit-plus” to piglets in the period before and after weaning to activate the protective systems of their body.

scholarly journals State of antioxidant protection system in carp hepatopancrease affected by associative Aeromonas Hydrophila infection with Saprolegnia Sp.

2020 ◽  
pp. 85-89
Author(s):  
Kh. Ya. Solopova ◽  
◽  
O. I. Vishchur ◽  
M. Z. Korylyak ◽  
I. Ye. Solovodzinska ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Aeromonas Hydrophila ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
The Body ◽  
Control Group ◽  
Research Station ◽  
Defense System ◽  
Peroxidation Processes ◽  
Experimental Group

Bacterial diseases are responsible for heavy mortality in both wild and cultured fish. Co-infections are very common in nature and occur when hosts are infected by two or more different pathogens either by simultaneous or secondary infections so that two or more infectious agents are active together in the same host. Associative infections pose a significant threat to fish farming, so it is necessary to develop effective means of prevention and treatment of these infections; it is advisable to study in detail their impact on various fish. The aim of the study was to determine the intensity of lipid peroxidation processes and the activity of the antioxidant defense system in the body of carp suffering from aeromonosis and the associated infection of aeromonosis with saprolegniosis. The research was conducted at the Lviv Research Station of the Institute of Fisheries of NAAS. There were three groups of fish, 5 in each. The control group consisted of clinically healthy fish, the first experimental group (D1) – carps affected by Aeromonas hydrophila infection, the second experimental group (D2), which consisted of carps affected by associative Aeromonas hydrophila infection with Saprolegnia Sp. The results of studies have shown that carp with associative infection ‒ of Aeromonas hydrophila infection with Saprolegnia Sp. leads to the activation of lipid peroxidation processes, especially the primary product of LPO. Thus, in the hepatopancreas of fish there was an increase of 1.4 (p <0.05) in the reasonable content of diene conjugates and there was a tendency to increase the content of TBA-active products. At the same time, the inhibitory effect of pathogenic factors of this disease on the activity of the enzymatic link of the antioxidant defense system was stated. In particular, carp patients with associative infection ‒ of Aeromonas hydrophila infection with Saprolegnia Sp. had higher catalase and superoxide dismutase activity, respectively, 1.3 (p <0.05) and 2.3 (p <0.01) times. The results of experimental studies made it possible to expand and deepen the mechanisms of the pathogenesis of the co-infection of Aeromonas hydrophila infection with Saprolegnia Sp. and to use the data to develop effective treatments.

scholarly journals Effect of the Supplement of Humic Origin on the Free Radical Processes and Histological Changes in the Tissues of Rats Affected by Chromium (VI)

2020 ◽  
Vol 11 (3) ◽  
pp. 10996-11008
Keyword(s):  
Body Weight ◽  
Free Radical ◽  
Antioxidant Defense ◽  
Reductase Activity ◽  
Antioxidant Defense System ◽  
Rat Tissues ◽  
Defense System ◽  
Free Radical Processes ◽  
Chromium Vi ◽  
Radical Processes

The goal of our study was to examine the effect of feed supplement Humilid on the antioxidant defense system, free radical processes, and histomorphological changes in the tissues of rats affected by Chromium (VI). Rats were divided into 4 groups. Animals of groups D2 and D3 received Humilid in a dose of 2 ml per kg of body weight for 28 days. From the 14th day of the experiment, rats of groups D1 and D2 were injected by K2Cr2O7 in a dose of 2 mg Cr per kg of body weight daily for 14 days. Control animals were injected with saline solution for 14 days. Results showed that Cr (VI) causes oxidative stress in rat tissues, accompanied by an increase in superoxide dismutase activity, lipid peroxidation products, and a decrease in glutathione peroxidase and glutathione reductase activity. Chromium also caused histopathological changes in the liver and kidney of rats. Watering of rats affected by Cr (VI) with Humilid leads to decreased oxidative processes, activation of the antioxidant defense system, and reduced pathological effects on animal tissues. Our results indicate the positive influence of Humilid on the rat’s organism, inhibiting free radical processes and exhibiting antioxidant, hepatoprotective, and adaptive properties.

MORPHOLOGICAL INDICATORS OF BLOOD, RESISTANCE OF THE ORGANISM AND MEAT PRODUCTIVITY OF PIGLET AT APPLICATION OF 20-HYDROXYEXDISONE

2020 ◽  
Vol 1 (3) ◽  
pp. 371-377
Author(s):  
A.G. Solovieva ◽  
◽  
K.T. Erimbetov ◽  
O.V. Obvintseva ◽  
◽  
...  
Keyword(s):  
Blood Plasma ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
The Body ◽  
Natural Resistance ◽  
Defense System ◽  
Growing Period ◽  
Meat Productivity ◽  
Experimental Group ◽  
In Blood Plasma

The effectiveness of using 20-hydroxyecdysone in the diet of piglets during the growing period and its effect on the formation of natural resistance, the antioxidant defense system of the body and their meat productivity were studied. The introduction of 20-hydroxyecdysone into the diet at the rate of 30 mg per kg of feed positively influenced on the bactericidal and lysozyme activity of blood serum, which in the experimental group were higher by 12,9 and 14,0%, respectively, compared with the control. The activity of superoxide dismutase and catalase enzymes in blood plasma in piglets of the experimental group was statistically significantly higher compared to the control. On the contrary, the content of malondialdehyde in blood plasma was statistically significantly lower in piglets of the experimental group compared with the control (17,1%, P≤0,01). In piglets of the experimental group, in comparison with the control, the output of muscle tissue (by 5,3%, P≤0,01), the area of the “muscle eye” (34,5%, P≤0,05) was statistically significantly higher, with less adipose tissue outlet.

scholarly journals Metabolic processes in the organism of animals under the action of plant extract

2019 ◽  
Vol 10 (2) ◽  
pp. 149-158 ◽  
Author(s):  
O. Buchko ◽  
V. Havryliak ◽  
O. Yaremkevych ◽  
R. Konechna ◽  
N. Ohorodnyk
Keyword(s):  
Body Weight ◽  
Protein Metabolism ◽  
Antioxidant Defense ◽  
Ethanolic Extract ◽  
Hemoglobin Concentration ◽  
Antioxidant Defense System ◽  
Biologically Active ◽  
Defense System ◽  
Radical Processes ◽  
Active Substances

The possibility of increasing metabolism and resistance in an organism of animals under the influence of biologically active substances of natural origin is considered in the paper. In the current study, we investigated the effect of 40% ethanolic extract of common nettle (Urtica dioica L.) on hematologic indexes (hemoglobin concentration, erythrocytes and leukocytes content), protein metabolism (total protein, aspartate aminotransferase (AST), alanine aminotransferase activities (ALT)), glutathione metabolism (peroxidase (GP), glutathione reductase (GR) and reduced glutathione content (GSH)), antioxidant defense systems (superoxide dismutase (SOD), catalase (CAT)), and indicators of free radical processes (lipid hydroperoxidation products (LHP), TBA-active products and carbonyl groups of protein (CP)) in the blood and tissues of normal and adrenalin-stressed rats. White Wistar rats (male) with body weight 180–200 g were used in the experiments. The animals were divided into 4 groups (control and 3 experimental), each containing 7 animals. The animals of experimental groups D2 and D3 received 40% ethanolic extract of common nettle in a dose of 5 mL/kg of body weight during 4 weeks. After 29 days of the experiment, the animals of D1 and D3 groups were intramuscularly administered 0.1% solution of adrenaline hydrochloride at a dose of 1 mg/kg of body weight. One day after the administration of adrenaline, the animals were decapitated under ether anesthesia. The objects of the study were blood and homogenates of liver, heart, and kidneys of the rats. Our results have shown that adrenaline stress causes a decrease in the concentration of hemoglobin, LHP, SOD activity and increase in activities of AST, GP, GR, and GSH content in the blood of rats. Stress induced by adrenaline was accompanied by decrease in CP content, activities of SOD, CAT, and increase in the glutathione chain of antioxidant defense system in the liver of the animals while in the heart and kidneys, there was an increase in oxidative stress parameters and decrease in antioxidant enzyme activities (SOD, CAT, GP, GR). The administration of ethanolic extract of nettle caused an increase within the physiological range in the erythrocytes and leucocytes content, hemoglobin concentration, and normalization of protein metabolism (decrease in the activity of AST and increase in protein concentration) in the blood of the stressed rats. The activation of the antioxidant defense system (increase in activities of SOD, GP, CAT, GR) and inhibition of free radical processes (decrease in concentration of LHP, TBA-active products and CP) was observed in the tissues of animals of D2 and D3 groups. Our results suggested that using a complex of biologically active substances of the common nettle ethanolic extract prevents metabolic disorders and stresses of different etiology through the activation of the antioxidant defense system, the normalization of protein metabolism and stimulation of hematopoiesis both in normal and stressed rats.

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