scholarly journals Use of an Animal Model to Evaluate Anxiolytic Effects of Dietary Supplementation with Tilia tomentosa Moench Bud Extracts

Nutrients ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3328
Author(s):  
Federica Turrini ◽  
Giulia Vallarino ◽  
Francesca Cisani ◽  
Dario Donno ◽  
Gabriele Loris Beccaro ◽  
...  
Keyword(s):  
Motor Coordination ◽  
Body Weight Gain ◽  
Daily Intake ◽  
Principal Component ◽  
The Body ◽  
Stress Disorders ◽  
And Gender ◽  
Spectrum Of Patients ◽  
Tilia Tomentosa

Anxiety disorders are common and complex psychiatric syndromes affecting a broad spectrum of patients. On top of that, we know that aging produces an increase in anxiety vulnerability and sedative consumption. Moreover, stress disorders frequently show a clear gender susceptibility. Currently, the approved pharmacological strategies have severe side effects such as hallucinations, addiction, suicide, insomnia, and loss of motor coordination. Dietary integration with supplements represents an intriguing strategy for improving the efficacy and the safety of synthetic anxiolytics. Accordingly, a recent article demonstrated that glyceric bud extracts from Tilia tomentosa Moench (TTBEs) exert effects that are consistent with anxiolytic activity. However, the effects of these compounds in vivo are unknown. To examine this question, we conducted behavioral analysis in mice. A total of 21 days of oral supplements (vehicle and TTBEs) were assessed by Light Dark and Hole Board tests in male and female mice (young, 3 months; old, 24 months). Interestingly, the principal component analysis revealed gender and age-specific behavioral modulations. Moreover, the diet integration with the botanicals did not modify the body weight gain and the daily intake of water. Our results support the use of TTBEs as dietary supplements for anxiolytic purposes and unveil age and gender-dependent responses.

scholarly journals Effect of Graded Levels of Spirulina (Arthropsira platensis) on Feed Intake and in vivo Digestibility of Trypsacum laxum in Guinea Pig (Cavia Porcellus L)

2019 ◽  
Vol 1 (1) ◽  
pp. 20-31
Author(s):  
Généviève Nguedia ◽  
Emile Miégoué ◽  
Fernand Tendonkeng ◽  
Mouchili Mama ◽  
Et Etienne Tedonkeng Pamo ◽  
...  
Keyword(s):  
Feed Intake ◽  
Guinea Pigs ◽  
Dry Matter ◽  
Body Weight Gain ◽  
Control Diet ◽  
Daily Intake ◽  
The Body ◽  
Average Weight ◽  
Apparent Digestibility

The intake and in vivo digestibility of Trypsacumlaxum in guinea pigs according to the graded level of spirulina was evaluated in Cameroon. 20 animals aged of 6 months with an average weight of 450 ± 50 g were randomly divided into 4 equivalent groups. Each group received T. laxum and 40g of compound feed containing 0% (TS0), 2% (TS2), 4% (TS4), and 6% (TS6) of spirulina. Feed intake was the different between the left over and the quantity served. The sample of T. laxum and those of each diet as well as feces were collected and analyzed for the apparent digestibility of each nutrient. Animals were weighed at the beginning and at the end of each period of the test to determine the body weight gain. This study showed that the average daily intake of dry matter (DM) for TS0, TS2, TS4 and TS6 was 74.39 ± 2.98, 78.66 ± 3.14, 83.89 ± 4.28 and 77.76 ± 4.40 g/head/day. The highest apparent digestibility coefficients of different nutrients were obtained with animals fed TS6 group while the lowest were observed in TS0.The apparent digestibility coefficient of dry matter (DM), organic matter (OM) and crude protein (CP) of the supplemented group were statistically higher than those of the control diet. Animals’ weight performances were statistically comparable between treatments. Thus, the combination of T. laxum with concentrated feed containing spirulina can be recommended for guinea-pigs, but the level of incorporation may not exceed 4% of its daily ration.

scholarly journals Anti-obesity effect of Tamarindus indicus seed extract against a high-fat diet-induced obese model in rats

2020 ◽  
Vol 11 (2) ◽  
pp. 2083-2089
Author(s):  
Nabeel K ◽  
Asra Fathima ◽  
Farhath Khanum ◽  
Manjula S N ◽  
Mruthunjaya K ◽  
...  
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Body Weight Gain ◽  
Seed Extract ◽  
The Body ◽  
High Fat ◽  
Cell Viability Assay ◽  
Serum Triglycerides

The present study was aimed to evaluate the anti-obesity property of Tamarindus indica seed extract (TSE) on high fat-fed obese rats. TSE was prepared by cold maceration method and qualitative phytochemical studies had been carried out. In vitro cell viability assay (MTT assay) was and oil red staining for evaluating the lipid accumulation in cells was carried out using 3T3-L1 cells, and leptin levels was evaluated by ELISA. In-vivo Obesity was induced in experimental rats by administration of a high-fat diet for 04 weeks. The anti-obesity effect was screened by oral administration of TSE at two different dose levels i.e., 250 and 500mg/kg b. Wt. Along with a high-fat diet for a period of 04 weeks. The anti-obesity activity is estimated in terms of body weight gain, serum triglycerides (TG), Total cholesterol (TC). In -vitro studies revealed that the TSE has no cytotoxic effect, Administration of a high-fat diet for 04 weeks significantly increased the body weight, serum triglycerides, cholesterol. Upon treatment with TSE, a significant dose-dependent alteration in body weight, triglycerides, cholesterol levels were observed, inferring the anti-obesity property of Tamarindus seed extract.

Adverse effects of lactational exposure to chlorpyrifos in suckling rats

2009 ◽  
Vol 29 (2) ◽  
pp. 77-92 ◽  
Author(s):  
SA Mansour ◽  
AH Mossa
Keyword(s):  
Body Weight ◽  
Adverse Effects ◽  
Body Weight Gain ◽  
Lethal Dose ◽  
Daily Intake ◽  
Oral Route ◽  
The Body ◽  
Lactational Exposure ◽  
Histopathological Alterations ◽  
Liver And Kidney

The present study was undertaken to evaluate the oxidative damage, biochemical and histopathological alterations in sucking rats whose mothers were exposed to the insecticide chlorpyrifos (CPF). Dams were administered CPF, via oral route. Doses equalled 0.01 mg kg—1 body weight (b.wt.; acceptable daily intake, ADI), 1.00 mg kg—1 b.wt. (no observed adverse effects level, NOAEL) and 1.35 mg kg—1 b.wt. (1/100 lethal dose [LD50]) from postnatal day 1 until day 20 after delivery. At two high doses of CPF, the body weight gain and relative liver and kidney weight of suckling pups were significantly decreased. Exposure of the mothers to CPF caused increase in lipid peroxidation (LPO) and decrease in superoxide dismutase (SOD) and glutathione-s-transferase (GST) in lactating pups. CPF altered the level of the marker parameters related to the liver and kidneys. Consistent histological changes were found in the liver and kidneys of the subjected pups, especially at the higher doses. The results suggested that the transfer of CPF intoxication through the mother’s milk has resulted in oxidative stress and biochemical and histopathological alterations in the suckling pups. The data of this study may be considered as a contribution to the problem of lactational transfer of the relatively less persistent OP pesticides, such as CPF.

scholarly journals The 3Rs: Reduction and refinement through a multivariate statistical analysis approach in a behavioural study to unveil anxiolytic effects of natural extracts of Tilia tomentosa

2020 ◽  
Author(s):  
Guendalina Olivero ◽  
Federica Turrini ◽  
Matteo Vergassola ◽  
Raffaella Boggia ◽  
Paola Zunin ◽  
...  
Keyword(s):  
Statistical Approach ◽  
Principal Component ◽  
Experimental Protocol ◽  
Multivariate Statistical ◽  
Data Set ◽  
Natural Extracts ◽  
Multivariate Statistical Approach ◽  
Behavioural Tests ◽  
Tilia Tomentosa

We propose a multivariate statistical approach based on Principal Component Analysis (PCA) as an useful instrument to improve the Rules of Refinement and Reduction in in vivo animal experimentation. We analysed with PCA the preliminary data from a study on the effects of the oral administration of Tilia tomentosa bud extracts (TTBEs) on the behavioural skills of adult and aged male and female mice. PCA allows to rationalize the data set information and to dissect the results, showing connections among variables under study (behavioural parameters) and different trends in the experimental groups (control and TTBEs-administered animals). Our results show that PCA can give some important information that can be useful for the refinement of the experimental protocol, in order to reduce the number of the animals used in the experiments and/or the behavioural tests to get reliable information.

scholarly journals Performance of Kampung Super chicken and Bangkok chicken first generation (G1) until the age of 12 weeks

2021 ◽  
Vol 19 (3) ◽  
pp. 326
Author(s):  
Fetty Febriana Rahayu ◽  
Depison Depison ◽  
Gushairiyanto Gushairiyanto
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
First Generation ◽  
Body Weight Gain ◽  
Principal Component ◽  
Selection Response ◽  
The Body ◽  
Body Measurements ◽  
Average Value ◽  
Research Material

<p class="MDPI17abstract"><strong>Objective: </strong>The purpose of this research was to determine the performance of the first generation (G1) Kampung Super and Bangkok chicken aged 12 weeks<span lang="EN-GB">.</span></p><p class="MDPI17abstract"><strong>Methods: </strong>The research material were G1 Kampung Super and Bangkok chicken with 174 samples for each strain. This research was using the experimental method. The data collected included body weight, body weight gain, body measurements, and selected responses. The body weight, body weight gain, and body measurements data were analyzed using t-test independent, while the average value vector of body measured using <em><span>T<sup>2</sup>-Hotelling test.</span></em> Principal Component Analysis ware used to identify the characteristics of body shape and size.<strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong>The results of this study showed that body weight, body weight gain of DOC-4 weeks, 4-8 weeks, 8-12 weeks, and body measurements of G1 Kampung Super chickens were real different higher (P&lt;0.05) than G1 Bangkok. The identifier of the size of the G1 Kampung Super and Bangkok chicken is the chest circumference. The characteristics of the shape G1 Kampung Super chicken is the length of the wings and the G1 Bangkok chicken is the lower body lenght. The selection response of G1 Kampung Super is higher than G1 Bangkok<span lang="EN-GB">.</span><strong></strong></p><p><strong><span>Conclusions: </span></strong>The<span> performance of G1 Kampung Super chicken was higher than G1 Bangkok chicken but the selection response value of G1 Bangkok chicken was higher than G1 Kampung Super chicken. The highest body weight gain of G1 Kampung Super and Bangkok chickens was achieved at the age of 8-12 weks</span><span lang="EN-GB">.</span></p>

scholarly journals IN VIVO ENRICHMENT OF THE LAMB OF ESSENTIAL TRACE ELEMENTS FOR ITS USE IN THE TECHNOLOGY OF FUNCTIONAL FOODS

2018 ◽  
Vol 3 (3) ◽  
pp. 74-88 ◽  
Author(s):  
Tatiana M. Giro ◽  
Ivan F. Gorlov ◽  
Marina I. Slozhenkina ◽  
Sergey V. Kozlov ◽  
Nogman V. Tasmuchanov
Keyword(s):  
Raw Materials ◽  
Daily Intake ◽  
Test Group ◽  
Zinc Content ◽  
Physiological Effect ◽  
Thyroid Stimulating Hormone ◽  
The Body ◽  
Laboratory Animals ◽  
Experimental Animals

To provide preventive measures for solving the problem of micronutrient deficiency, a new effective biologically safe method of enrichment lamb through the intervention of the cattle rations with feed supplements containing iodine and selenium in organic form has been developed. An increase in the iodine, selenium and zinc content in meat of the test animals fed enriched diets was noted, with more significant localization of elements to be registered in the meat from the ram lamb fed the «Yoddar-Zn» feed supplement together with organic selen preparation «DAFS-25» (Zn - 980 mg/100g; Se — 53.9 mg/100 g; I — 77.6 mg/100 g). So, 100 g of m. longissimus dorsi contained 8.2; 77.0 and 51.7 % of the recommended daily intake of these elements, respectively, that proved the feasibility of further output of the functional products with balanced essential nutrients. Based on the data on greater localization of microelements in meat from the ram lamb fed «Yoddar-Zn» with «DAFS-25», we made jerked snacks from the raw materials obtained from the animals of this test group. In processing, the zinc content in the product decreased by 21.3 %, iodine — 26.5 %, selenium — 16.3 % and made 771 mg/100 g, 39.72 mg/100 g, and 57.01 mg/100 g, respectively, that met the daily norm for 6.4; 56.7 and 38.0 % (for 100 g of product). In the vacuum-packed snacks, the loss of zinc was 11.3 %, iodine — 14.3 %, selenium — 12.6 % from the original content. After 3 months of storage, the zinc content in 100 g of the finished products was 5.7; selenium — 49.6; iodine — 32.6 % of the daily requirement. Investigations of physiological effect of the jerked snacks on the body of the laboratory animals were performed. Stimulating effect of selenium and iodine on the hematopoietic function of the experimental animals was revealed. The study of the functional activity of the thyroid gland found that the experimental animals’ content of thyroid stimulating hormone in blood serum was significantly higher than that of the control rats.

scholarly journals N-acetylcysteine+nimesulide: An association strategy aiming to prevent nimesulide-induced hepatotoxicity

2021 ◽  
Vol 19 (2) ◽  
pp. 91-99
Author(s):  
Amanda G. Elias ◽  
Julia S. da Silva ◽  
Rafaela L. Klein ◽  
Francieli U. I. Amaral ◽  
Marcelo D. Arbo ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Cell Model ◽  
Body Weight Gain ◽  
The Body ◽  
Relative Mass ◽  
Male Wistar Rats ◽  
Control Body

Introduction: Nimesulide is a potent anti-inflammatorywith rapid and long-lasting effects, but also with a high riskof hepatotoxicity. Objective: This work aimed to preventnimesulide-induced hepatotoxicity through the associationof nimesulide with a hepatoprotective agent. Materials andMethods: First, we tested three hepatoprotective agents:N-acetylcysteine, L-carnitine, and Gingko biloba extract inan in vitro hepatic cell model. Both N-acetylcysteine and G.biloba showed promisor results. We selected N-acetylcysteineto continue the studies in an animal model. In vivo study wasperformed using male Wistar rats divided in 4 groups: control,nimesulide (100mg/kg/day), nimesulide (100mg/kg/day) +N-acetylcysteine (100mg/kg/day) and N-acetylcysteine alone(100mg/kg/day). Treatments were given by gavage, daily, for15 days. Results: Animals receiving nimesulide alone showedlower body weight gain compared to control. Body weightgain in the nimesulide + N-acetylcysteine group was higherthan nimesulide alone, evidencing lower toxicity. However,the body weight gain of the nimesulide + N-acetylcysteinegroup was still lower than the control animals. Animals treatedwith nimesulide alone presented an increased relative mass ofheart, liver, and spleen and significant hepatic damage seen inmicroscopy when compared to other groups. N-acetylcysteineco-administered with nimesulide prevented the increasedheart mass, but the same was not true with liver and spleen.Conclusions: This work evidence partial protection elicitedby the association of N-acetylcysteine and nimesulide againstnimesulide-induced hepatotoxicity.

Cadmium: exposure markers as predictors of nephrotoxic effects

1994 ◽  
Vol 40 (7) ◽  
pp. 1391-1394 ◽  
Author(s):  
R R Lauwerys ◽  
A M Bernard ◽  
H A Roels ◽  
J P Buchet
Keyword(s):  
Daily Intake ◽  
Epidemiologic Studies ◽  
The Body ◽  
Biologically Active ◽  
Cross Sectional ◽  
Limit Value ◽  
The Us ◽  
Biological Limit Value ◽  
Low Exposure

Abstract Cadmium (Cd) is a cumulative element with a biological half-life of &gt; 10 years in humans. The total amount of Cd accumulated in the liver and in the kidney can be measured in vivo by neutron activation (or x-ray fluorescence), but this technique does not necessarily measure the fraction that is biologically active. At low exposure (i.e., general environmental exposure or moderate occupational exposure), blood Cd is mainly influenced by the last 2 to 3 months of exposure. Under such conditions, the Cd concentration in urine mainly reflects the amount of Cd stored in the body, particularly in the kidney. In Europe and the US, the Cd reference values are usually &lt; 2 nmol/mmol creatinine. Because most of the Cd in urine is probably bound to metallothionein, the changes in the urinary metallothionein concentration parallel those of Cd. The determination of Cd concentration in hair is of limited value because in humans it is difficult to distinguish between externally deposited and endogenous Cd. Fecal Cd is a good indicator of the oral daily intake. The results of several cross-sectional epidemiologic studies of the relation between the prevalence of renal dysfunction and Cd concentration in urine led us to propose a biological limit value for Cd of 5 and 2 nmol/mmol creatine for adult male workers and the general population, respectively.

The Role of Polyphenols in the Modulation of Plasma Non-Enzymatic Antioxidant Capacity (NEAC)

2012 ◽  
Vol 82 (3) ◽  
pp. 228-232 ◽  
Author(s):  
Mauro Serafini ◽  
Giuseppa Morabito
Keyword(s):  
Antioxidant Capacity ◽  
Dietary Intervention ◽  
Ex Vivo ◽  
The Body ◽  
Dietary Polyphenols ◽  
Enzymatic Antioxidant ◽  
Endogenous Antioxidant

Dietary polyphenols have been shown to scavenge free radicals, modulating cellular redox transcription factors in different in vitro and ex vivo models. Dietary intervention studies have shown that consumption of plant foods modulates plasma Non-Enzymatic Antioxidant Capacity (NEAC), a biomarker of the endogenous antioxidant network, in human subjects. However, the identification of the molecules responsible for this effect are yet to be obtained and evidences of an antioxidant in vivo action of polyphenols are conflicting. There is a clear discrepancy between polyphenols (PP) concentration in body fluids and the extent of increase of plasma NEAC. The low degree of absorption and the extensive metabolism of PP within the body have raised questions about their contribution to the endogenous antioxidant network. This work will discuss the role of polyphenols from galenic preparation, food extracts, and selected dietary sources as modulators of plasma NEAC in humans.

L-Ascorbic Acid Addition to Chitosan Reduces Body Weight in Overweight Women

2014 ◽  
Vol 84 (1-2) ◽  
pp. 5-11 ◽  
Author(s):  
Eun Y. Jung ◽  
Sung C. Jun ◽  
Un J. Chang ◽  
Hyung J. Suh
Keyword(s):  
Ascorbic Acid ◽  
Body Weight ◽  
Fat Body ◽  
Body Weight Gain ◽  
Skinfold Thickness ◽  
The Body ◽  
Control Group ◽  
Percentage Body Fat ◽  
Overweight Women ◽  
Body Weights

Previously, we have found that the addition of L-ascorbic acid to chitosan enhanced the reduction in body weight gain in guinea pigs fed a high-fat diet. We hypothesized that the addition of L-ascorbic acid to chitosan would accelerate the reduction of body weight in humans, similar to the animal model. Overweight subjects administered chitosan with or without L-ascorbic acid for 8 weeks, were assigned to three groups: Control group (N = 26, placebo, vehicle only), Chito group (N = 27, 3 g/day chitosan), and Chito-vita group (N = 27, 3 g/day chitosan plus 2 g/day L-ascorbic acid). The body weights and body mass index (BMI) of the Chito and Chito-vita groups decreased significantly (p < 0.05) compared to the Control group. The BMI of the Chito-vita group decreased significantly compared to the Chito group (Chito: -1.0 kg/m2 vs. Chito-vita: -1.6 kg/m2, p < 0.05). The results showed that the chitosan enhanced reduction of body weight and BMI was accentuated by the addition of L-ascorbic acid. The fat mass, percentage body fat, body circumference, and skinfold thickness in the Chito and Chito-vita groups decreased more than the Control group; however, these parameters were not significantly different between the three groups. Chitosan combined with L-ascorbic acid may be useful for controlling body weight.

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