scholarly journals Biocomposite Hydrogels for the Treatment of Bacterial Infections: Physicochemical Characterization and In Vitro Assessment

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2079
Author(s):  
Delia Mihaela Rata ◽  
Anca Niculina Cadinoiu ◽  
Marcel Popa ◽  
Leonard Ionut Atanase ◽  
Oana Maria Daraba ◽  
...  
Keyword(s):  
Zno Nanoparticles ◽  
Bacterial Infections ◽  
Physicochemical Characterization ◽  
Antimicrobial Properties ◽  
In Vitro Cytotoxicity ◽  
Inorganic Nanoparticles ◽  
Poly Vinyl Alcohol ◽  
Swelling Degree ◽  
Covalent Crosslinking

Hydrogels based on natural and synthetic polymers and inorganic nanoparticles proved to be a viable strategy in the fight against some Gram-positive and Gram-negative bacteria. Additionally, numerous studies have demonstrated the advantages of using ZnO nanoparticles in medicine due to their high antibacterial efficacy and relatively low cost. Consequently, the purpose of our study was to incorporate ZnO nanoparticles into chitosan/poly (vinyl alcohol)-based hydrogels in order to obtain a biocomposite with antimicrobial properties. These biocomposite hydrogels, prepared by a double crosslinking (covalent and ionic) were characterized from a structural, morphological, swelling degree, and mechanical point of view. FTIR spectroscopy demonstrated both the apparition of new imine and acetal bonds due to covalent crosslinking and the presence of the sulfate group following ionic crosslinking. The morphology, swelling degree, and mechanical properties of the obtained hydrogels were influenced by both the degree of covalent crosslinking and the amount of ZnO nanoparticles incorporated. In vitro cytotoxicity assessment showed that hydrogels without ZnONPs are non-cytotoxic while the biocomposite hydrogels are weak (with 3% ZnONPs) or moderately (with 4 and 5% ZnONPs) cytotoxic. Compared to nanoparticle-free hydrogels, the biocomposite hydrogels show significant antimicrobial activity against S. aureus, E. coli, and K. pneumonia.

scholarly journals Development and physicochemical characterization of novel porous phosphate glass bone graft substitute and in vitro comparison with xenograft

2021 ◽  
Vol 32 (6) ◽  
Author(s):  
Niketa Chauhan ◽  
Nilay Lakhkar ◽  
Amol Chaudhari
Keyword(s):  
Cell Proliferation ◽  
Physicochemical Properties ◽  
Bone Graft ◽  
Phosphate Glass ◽  
Physicochemical Characterization ◽  
Bone Graft Substitute ◽  
Organic Content ◽  
In Vitro Cytotoxicity ◽  
Porous Phosphate

AbstractThe process of bone regeneration in bone grafting procedures is greatly influenced by the physicochemical properties of the bone graft substitute. In this study, porous phosphate glass (PPG) morsels were developed and their physicochemical properties such as degradation, crystallinity, organic content, surface topography, particle size and porosity were evaluated using various analytical methods. The in vitro cytotoxicity of the PPG morsels was assessed and the interaction of the PPG morsels with Dental Pulp Stem Cells (DPSCs) was studied by measuring cell proliferation and cell penetration depth. The cell-material interactions between PPG morsels and a commercially available xenograft (XG) were compared. The PPG morsels were observed to be amorphous, biocompatible and highly porous (porosity = 58.45%). From in vitro experiments, PPG morsels were observed to be non-cytotoxic and showed better cell proliferation. The internal surface of PPG was easily accessible to the cells compared to XG.

Novel Pluronic F‐127‐coated ZnO nanoparticles: Synthesis, characterization, and their in‐vitro cytotoxicity evaluation

2021 ◽  
Author(s):  
Abdulsalam Abuelsamen ◽  
Shahrom Mahmud ◽  
Noor Haida Mohd Kaus ◽  
Omar F. Farhat ◽  
Sabah M. Mohammad ◽  
...  
Keyword(s):  
Zno Nanoparticles ◽  
In Vitro Cytotoxicity ◽  
Nanoparticles Synthesis

scholarly journals Multifunctional Coatings for Robotic Implanted Device

2019 ◽  
Vol 20 (20) ◽  
pp. 5126 ◽  
Author(s):  
Caterina Cristallini ◽  
Serena Danti ◽  
Bahareh Azimi ◽  
Veronika Tempesti ◽  
Claudio Ricci ◽  
...  
Keyword(s):  
Acrylic Acid ◽  
Vinyl Alcohol ◽  
Soft Tissues ◽  
Functional Characterization ◽  
Titanium Substrate ◽  
Repair Process ◽  
Chemical Imaging ◽  
In Vitro Cytotoxicity ◽  
Poly Vinyl Alcohol

The objective of this study was the preparation and physico-chemical, mechanical, biological, and functional characterization of a multifunctional coating for an innovative, fully implantable device. The multifunctional coating was designed to have three fundamental properties: adhesion to device, close mechanical resemblance to human soft tissues, and control of the inflammatory response and tissue repair process. This aim was fulfilled by preparing a multilayered coating based on three components: a hydrophilic primer to allow device adhesion, a poly(vinyl alcohol) hydrogel layer to provide good mechanical compliance with the human tissue, and a layer of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) fibers. The use of biopolymer fibers offered the potential for a long-term interface able to modulate the release of an anti-inflammatory drug (dexamethasone), thus contrasting acute and chronic inflammation response following device implantation. Two copolymers, poly(vinyl acetate-acrylic acid) and poly(vinyl alcohol-acrylic acid), were synthetized and characterized using thermal analysis (DSC, TGA), Fourier transform infrared spectroscopy (FT-IR chemical imaging), in vitro cell viability, and an adhesion test. The resulting hydrogels were biocompatible, biostable, mechanically compatible with soft tissues, and able to incorporate and release the drug. Finally, the multifunctional coating showed a good adhesion to titanium substrate, no in vitro cytotoxicity, and a prolonged and controlled drug release.

scholarly journals Synthesis and Antimicrobial Properties of Highly Cross-Linked pH-Sensitive Hydrogels through Gamma Radiation

Polymers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 2223
Author(s):  
Moises Bustamante-Torres ◽  
Victor H. Pino-Ramos ◽  
David Romero-Fierro ◽  
Sandra P. Hidalgo-Bonilla ◽  
Héctor Magaña ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Film Formation ◽  
Antimicrobial Properties ◽  
Ph Sensitive ◽  
Scanning Calorimetry ◽  
Polymeric Systems ◽  
E Coli ◽  
High Prevalence

The design of new polymeric systems for antimicrobial drug release focused on medical/surgical procedures is of great interest in the biomedical area due to the high prevalence of bacterial infections in patients with wounds or burns. For this reason, in this work, we present a new design of pH-sensitive hydrogels copolymerized by a graft polymerization method (gamma rays), intended for localized prophylactic release of ciprofloxacin and silver nanoparticles (AgNPs) for potential topical bacterial infections. The synthesized hydrogels were copolymerized from acrylic acid (AAc) and agar. Cross-linked hydrogel film formation depended on monomer concentrations and the degree of radiation used (Cobalt-60). The obtained hydrogel films were characterized by attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and mechanical testing. The swelling of the hydrogels was evidenced by the influence of their pH-sensitiveness. The hydrogel was loaded with antimicrobial agents (AgNPs or ciprofloxacin), and their related activity was evaluated. Finally, the antimicrobial activity of biocidal-loaded hydrogel was tested against Escherichia coli (E. coli) and methicillin-resistant Staphylococcus aureus (MRSA) on in vitro conditions.

scholarly journals Essential oil of the leaves of Ricinus communis L.: In vitro cytotoxicity and antimicrobial properties

2012 ◽  
Vol 11 (1) ◽  
pp. 102 ◽  
Author(s):  
Zied Zarai ◽  
Ines Chobba ◽  
Riadh Mansour ◽  
Ahmed Békir ◽  
Néji Gharsallah ◽  
...  
Keyword(s):  
Essential Oil ◽  
Ricinus Communis ◽  
Antimicrobial Properties ◽  
In Vitro Cytotoxicity ◽  
Ricinus Communis L

scholarly journals Perillaldehyde 1,2-epoxide Loaded SLN-Tailored mAb: Production, Physicochemical Characterization and In Vitro Cytotoxicity Profile in MCF-7 Cell Lines

Pharmaceutics ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 161 ◽  
Author(s):  
Eliana B. Souto ◽  
Selma B. Souto ◽  
Aleksandra Zielinska ◽  
Alessandra Durazzo ◽  
Massimo Lucarini ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cetyltrimethylammonium Bromide ◽  
Physicochemical Characterization ◽  
Thiobarbituric Acid ◽  
In Vitro Cytotoxicity ◽  
Solid Lipid Nanoparticle ◽  
Mab Production ◽  
Epithelial Growth ◽  
Mcf 7

We have developed a new cationic solid lipid nanoparticle (SLN) formulation, composed of Compritol ATO 888, poloxamer 188 and cetyltrimethylammonium bromide (CTAB), to load perillaldehyde 1,2-epoxide, and surface-tailored with a monoclonal antibody for site-specific targeting of human epithelial growth receptor 2 (HER2). Perillaldehyde 1,2-epoxide-loaded cationic SLN (cPa-SLN), with a mean particle size (z-Ave) of 275.31 ± 4.78 nm and polydispersity index (PI) of 0.303 ± 0.081, were produced by high shear homogenization. An encapsulation efficiency of cPa-SLN above 80% was achieved. The release of perillaldehyde 1,2-epoxide from cationic SLN followed the Korsemeyer–Peppas kinetic model, which is typically seen in nanoparticle formulations. The lipid peroxidation of cPa-SLN was assessed by the capacity to produce thiobarbituric acid-reactive substances, while the antioxidant activity was determined by the capacity to scavenge the stable radical DPPH. The surface functionalization of cPa-SLN with the antibody was done via streptavidin-biotin interaction, monitoring z-Ave, PI and ZP of the obtained assembly (cPa-SLN-SAb), as well as its stability in phosphate buffer. The effect of plain cationic SLN (c-SLN, monoterpene free), cPa-SLN and cPa-SLN-SAb onto the MCF-7 cell lines was evaluated in a concentration range from 0.01 to 0.1 mg/mL, confirming that streptavidin adsorption onto cPa-SLN-SAb improved the cell viability in comparison to the cationic cPa-SLN.

scholarly journals Encapsulation of Babchi Oil in Cyclodextrin-Based Nanosponges: Physicochemical Characterization, Photodegradation, and In Vitro Cytotoxicity Studies

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 169 ◽  
Author(s):  
Sunil Kumar ◽  
Pooja ◽  
Francesco Trotta ◽  
Rekha Rao
Keyword(s):  
Essential Oil ◽  
Physicochemical Characterization ◽  
In Vitro Cytotoxicity ◽  
Traditional Medicines ◽  
Molar Ratios ◽  
Cyclodextrin Nanosponges ◽  
Cytotoxicity Studies ◽  
The Fourier Transform ◽  
Photo Stability

Babchi (Psoralea corylifolia) oil is an important essential oil used in several traditional medicines to cure various disorders. This phytotherapeutic agent possesses a number of pharmacological activities including antibacterial, antifungal, antioxidant, anti-inflammatory, immunomodulatory, and antitumor factors. However, volatile nature, poor stability, and solubility of babchi oil (BO) restrict its pharmaceutical applications. Therefore, the aim of the present work was to encapsulate this oil in β-cyclodextrin nanosponges (NS) in order to overcome the above limitations. To fabricate nanosponges, β-cyclodextrin was cross-linked with diphenyl carbonate in different molar ratios viz. 1:2, 1:4, 1:6, 1:8, and 1:10. The blank nanosponges were loaded with BO using the freeze-drying method. The particle size of the BO loaded nanosponges was found to lie between 200 and 500 nm with low polydispersity index. Furthermore, the zeta potential, the Fourier transform infrared spectroscopy, X-ray diffraction, thermal analysis, and electron microscopy were carried out for characterization of BO nanosponges. Results obtained from spectral analysis ascertained the formation of inclusion complexes. Additionally, solubilisation efficiency of BO was checked in distilled water and found enhanced by 4.95 times with optimized β-cyclodextrin nanosponges. The cytotoxicity study was carried out by the MTT assay using HaCaT cell lines. A significant improvement in photo-stability of essential oil was also observed by inclusion innanosponges. Lastly, the optimized formulation was tested for antibacterial activity using Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. Therefore, encapsulation of BO in nanosponges resulted in efficacious carrier system in terms of solubility, photo-stability, and safety of this oil along with handling benefits.

scholarly journals Modifications of Hydroxyapatite by Gallium and Silver Ions—Physicochemical Characterization, Cytotoxicity and Antibacterial Evaluation

2020 ◽  
Vol 21 (14) ◽  
pp. 5006
Author(s):  
Kamil Pajor ◽  
Łukasz Pajchel ◽  
Anna Zgadzaj ◽  
Urszula Piotrowska ◽  
Joanna Kolmas
Keyword(s):  
Solid State ◽  
Physicochemical Characterization ◽  
Silver Ions ◽  
In Vitro Cytotoxicity ◽  
Emission Spectrometry ◽  
Resonance Spectroscopy ◽  
Hydroxyl Groups ◽  
Solid State Method ◽  
State Method

Hydroxyapatite (HA) powders enriched with silver or gallium ions or both were synthesized by two different routes: standard precipitation and the solid-state method. The powders were characterized by using several methods: inductively coupled plasma optical emission spectrometry (ICP-OES), powder X-ray diffractometry (PXRD), transmission electron microscopy (TEM), infrared spectroscopy (FT-IR) and solid-state nuclear magnetic resonance spectroscopy (ssNMR). The effects of enrichment of the HAs in Ag+ or Ga3+ or both on in vitro cytotoxicity and microbiological activity were discussed. PXRD experiments showed that the samples obtained by the wet method consisted of single-phase nanocrystalline HA, while the samples prepared via the solid-state method are microcrystalline with a small amount of calcium oxide. The introduction of higher amounts of silver ions was found to be more effective than enriching HA with small amounts of Ag+. Gallium and silver ions were found not to affect the lattice parameters. Ga3+ affected the crystallinity of the samples as well as the content of structural hydroxyl groups. Among samples synthesized by the wet method, only one (5Ag-HAw) was cytotoxic, whereas all Ga-containing samples obtained by the dry method showed cytotoxicity. In the preliminary antimicrobial test all the materials containing “foreign” ions showed high antibacterial activity.

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